补肾中药对卵细胞体外成熟的影响
|
摘要
目的探讨含补肾中药鼠血清对小鼠卵细胞体外成熟的影响及其机制。
方法未成熟卵细胞来自孕马血清促性腺激素(PMSG)刺激后的90只ICR小鼠的卵巢。将收集的小鼠卵丘细胞-卵母细胞复合体(COCs)245个随机分为含补肾中药鼠血清1#、2#、3#组、空白鼠血清组、空白组,分别置入添加了2%含补肾中药鼠血清1#、2#、3#、2%空白鼠血清及未添加鼠血清的M199培养液中,采用微滴法培养24h,体视显微镜和倒置显微镜观察并计算小鼠卵细胞体外第一极体(PB1)排出率即成熟率。将COCs约238个随机分为含药鼠血清1#组、卵泡刺激素(FSH)组、空白鼠血清组、空白组,分别置入添加了2%含药鼠血清1#、100IU/L FSH、2%空白鼠血清及未添加鼠血清的M199培养液中,显微镜观察并计算小鼠卵细胞体外成熟率、受精率及卵裂率,实验重复3次。将COCs随机分为含药鼠血清1#组、卵泡刺激素(FSH)组、空白鼠血清组、空白组,免疫荧光标记法结合反射荧光显微镜观察细胞骨架指标(纺锤体、染色体),及成熟促进因子(MPF)调节亚基周期蛋白B(CyclinB)的表达；荧光检测仪定量分析Cyclin B1的表达强度。将COCs随机分为含药鼠血清1#组、空白鼠血清组,半定量RT-PCR检测卯母细胞MPF调节亚基Cyclin Bl mRNA的表达。采用酶联免疫法测定含药鼠血清1#、2#、3#与空白鼠血清FSH和雌二醇(E2)水平。
结果
1.含药鼠血清1#、2#组均较空白组卵细胞成熟率明显增高(42.31%、36.17% vs 16.67%,分别P<0.01、P<0.05);含药鼠血清1#组较空白鼠血清组卵细胞成熟率明显增高(42.31% vs 22.92%,P<0.05)；含药鼠血清1#组较空白组生发泡破裂和第一极体(GVBD+PBl)出现率明显增高(84.62% vs 66.67%,P<0.05)；含药鼠血清各组之间GVBD+PBl和成熟率比较,无显著性差异。
2.FSH组、含药鼠血清1#组均较空白组卵细胞成熟率明显增高(33.69%、40.98% vs 17.71%,P<0.01)：FSH组、含药鼠血清1#组均较空白鼠血清组卵细胞成熟率明显增高(33.69%、40.98% vs 22.81%,分别P<0.05、P<0.01)；FSH组、含药鼠血清1#组均较空白组GVBD+PBl明显增高(80.75%、83.61% vs 64.00%,P<0.01)：FSH组、含药鼠血清1#组均较空白鼠血清组GVBD+PBl明显增高(80.75%、83.61% vs 71.35%,分别P<0.05、P<0.01);FSH组与含药鼠血清1#组GVBD+PBl和成熟率比较,无显著性差异;各组之间受精率及卵裂率总体上无明显差异。
3次重复实验分次结果显示基本一致。FSH组、含药鼠血清1#组均较空白组卵细胞成熟率明显增高,分别P<0.05、P<0.05或P<0.01；含药鼠血清1#组均较空白血清组卵细胞成熟率明显增高,P<0.05;FSH组、含药鼠血清1#组均较空白组GVBD+PBl明显增高,分别P<0.05、P<0.05或P<0.01；FSH与含药鼠血清1#组GVBD+PBl和成熟率比较,无显著性差异。
3.FSH、含药鼠血清1#组CyclinBl的荧光强度较空白组、空白鼠血清组明显增强(15069.00±3908.88、17936.50±4717.42 vs 5029.50±1656.61、6625.33±1777.41,P<0.01);FSH与含药鼠血清1#组比较,无显著性差异。含药鼠血清1#组Cyclin Bl mRNA的表达(Cyclin Bl/GAPDH=2.78)较空白鼠血清组(Cyclin Bl/GAPDH=2)增强。空白组、空白鼠血清组、FSH组、含药鼠血清1#组卵子骨架各组之间比较,无显著性差异。含药鼠血清1#、2#、3#组血清FSH和E2水平均较空白鼠血清组高,无显著性差异。
结论含补肾中药鼠血.清1#体外可以促进小鼠未成熟卵细胞生长发育,主要是促进卵细胞核成熟,并且不增加成熟卵细胞细胞骨架的异常几率。其机制与增强卵细胞成熟促进因子调节亚基Cvclin Bl的表达有关。
Objective To study the effect of traditional chinese medicine for reinforcing the kidney on mouse oocyte in vitro maturation.
Method Inmature oocytes were retreived from 90 ICR murine ovaries stimulated by pregnant mare serum gonadotropin (PMSG).245 mouse cumulus oocyte complexes (COCs) were collected and randomly divided into five groups, rat serum containing traditional chinese medicine 1#,2#,3# for reinforcing the kidney, blank serum(rat serum without traditional chinese medicine) and blank group, and microdrop matured in M199 media for 24 h with 2% rat serum containing traditional chinese medicine 1#,2#,3# for reinforcing the kidney,2% blank serum and medium without rat serum, separately. Oocyte maturation rate were counted. About 238 mouse COCs were collected and randomly divided into four groups, medicated serum 1#, follicle-stimulating hormone (FSH), blank serum and blank group, and matured in media with 2% medicated serum 1#,100IU/L FSH,2% blank serum and medium without rat serum, separately. Oocyte maturation rate, fertilization rate and cleavage rate were counted. The experiment was repeated 3 times. The mouse COCs were collected and randomly divided into four groups, medicated serum 1#, follicle-stimulating hormone (FSH), blank serum and blank group. The expression of oocyte cytoskeleton (spindle and chromosome) and regulatory subunit Cyclin B of mature promoting factor (MPF) were detected by immunofluorescence and observed by reflection fluorescence microscope. And the expression intensity of Cyclin B1 was analyzed by fluorescence testing instrument. The mouse COCs were collected and randomly divided into two groups, medicated serum 1# and blank serum group. Semi-quantity RT-PCR method was used to observe the expression of Cyclin B1 mRNA in mouse oocytes. FSH and E2 in rat blood were determinated by enzyme immunoassay (EIA).
Results
1. The oocyte maturation rate in the medicated serum 1#,2# groups were higher than that in the blank group (42.31%、36.17% vs 16.67%, P<0.01 or P<0.05). The oocyte maturation rate in the medicated serum 1# group was higher than that in the blank serum group (42.31% vs 22.92%, P<0.05). germinal vesicle breakdown and the first polar body (GVBD+PB1) in the medicated serum 1# group was higher than that in the blank group (84.62% vs 66.67%, P<0.05). There was no significant difference between the medicated serum 1#,2#,3# groups.
2. The oocyte maturation rate in the FSH group and the medicated serum 1# group were higher than that in the blank group (33.69%,40.98% vs 17.71%, P<0.01), The oocyte maturation rate in the FSH group and the medicated serum 1# group were higher than that in the blank serum group (33.69%、40.98% vs 22.81%, P <0.05 or P<0.01). GVBD+PB1 in the FSH group and the medicated serum 1# group were higher than that in the blank group (80.75%、83.61% vs 64.00%, P<0.01). GVBD+PB1 in the FSH group and the medicated serum 1# group were higher than that in the blank serum group (80.75%、83.61% vs 71.35%, P<0.05 or P<0.01) There was no significant difference between the FSH group and the medicated serum 1# group.
The experiment was repeated threel times to get the basically consistent outcome. The oocyte maturation rate in the FSH group and the medicated serum 1# group were higher than that in the blank group, respectively, P<0.05, P<0.05 or P<0.01. The oocyte maturation rate in the FSH group and the medicated serum 1" group were higher than that in the blank serum group, P<0.05. GVBD+PB1 in the FSH group and the medicated serum 1# group were higher than that in the blank group, respectively, P<0.05、P<0.05 or P<0.01. There was no significant difference between the FSH group and the medicated serum 1# group.
3. Fluorescence intensity of Cyclin B1 in the FSH group and the medicated serum 1# group were higher than that in the blank group and the blank serum group (15069.00±3908.88,17936.50±4717.42 vs 5029.50±1656.61、6625.33±1777.41, P<0.01).There was no significant difference between the FSH group and the medicated serum 1" group. the expression of Cyclin B1 mRNA in the medicated serum 1# group (Cyclin B1/GAPDH=2.78) were higher than that in the blank serum group (Cyclin B1/GAPDH=2) There was no significant, difference of oocyte cytoskeleton between medicated serum 1", follicle-stimulating hormone (FSH), blank serum and blank group. The levels of FSH and E2 in the medicated serum 1#,2#,3# groups were higher than that in the blank serum group, but there was no significant difference between the four groups.
Conclusion Rat serum containing traditional chinese medicine for reinforcing the kidney 1# has the effect of improving the mouse oocyte development in vitro. The medicated serum 1# has no adverse effect on the cytoskeleton configuration of the mature oocytes. The effect of medicated serum 1" for improving the mouse oocyte in vitro maturation may be ralated to the effect on increasing the expression of maturation promoting factor regulatory subunit Cyclin B1.
方法未成熟卵细胞来自孕马血清促性腺激素(PMSG)刺激后的90只ICR小鼠的卵巢。将收集的小鼠卵丘细胞-卵母细胞复合体(COCs)245个随机分为含补肾中药鼠血清1#、2#、3#组、空白鼠血清组、空白组,分别置入添加了2%含补肾中药鼠血清1#、2#、3#、2%空白鼠血清及未添加鼠血清的M199培养液中,采用微滴法培养24h,体视显微镜和倒置显微镜观察并计算小鼠卵细胞体外第一极体(PB1)排出率即成熟率。将COCs约238个随机分为含药鼠血清1#组、卵泡刺激素(FSH)组、空白鼠血清组、空白组,分别置入添加了2%含药鼠血清1#、100IU/L FSH、2%空白鼠血清及未添加鼠血清的M199培养液中,显微镜观察并计算小鼠卵细胞体外成熟率、受精率及卵裂率,实验重复3次。将COCs随机分为含药鼠血清1#组、卵泡刺激素(FSH)组、空白鼠血清组、空白组,免疫荧光标记法结合反射荧光显微镜观察细胞骨架指标(纺锤体、染色体),及成熟促进因子(MPF)调节亚基周期蛋白B(CyclinB)的表达；荧光检测仪定量分析Cyclin B1的表达强度。将COCs随机分为含药鼠血清1#组、空白鼠血清组,半定量RT-PCR检测卯母细胞MPF调节亚基Cyclin Bl mRNA的表达。采用酶联免疫法测定含药鼠血清1#、2#、3#与空白鼠血清FSH和雌二醇(E2)水平。
结果
1.含药鼠血清1#、2#组均较空白组卵细胞成熟率明显增高(42.31%、36.17% vs 16.67%,分别P<0.01、P<0.05);含药鼠血清1#组较空白鼠血清组卵细胞成熟率明显增高(42.31% vs 22.92%,P<0.05)；含药鼠血清1#组较空白组生发泡破裂和第一极体(GVBD+PBl)出现率明显增高(84.62% vs 66.67%,P<0.05)；含药鼠血清各组之间GVBD+PBl和成熟率比较,无显著性差异。
2.FSH组、含药鼠血清1#组均较空白组卵细胞成熟率明显增高(33.69%、40.98% vs 17.71%,P<0.01)：FSH组、含药鼠血清1#组均较空白鼠血清组卵细胞成熟率明显增高(33.69%、40.98% vs 22.81%,分别P<0.05、P<0.01)；FSH组、含药鼠血清1#组均较空白组GVBD+PBl明显增高(80.75%、83.61% vs 64.00%,P<0.01)：FSH组、含药鼠血清1#组均较空白鼠血清组GVBD+PBl明显增高(80.75%、83.61% vs 71.35%,分别P<0.05、P<0.01);FSH组与含药鼠血清1#组GVBD+PBl和成熟率比较,无显著性差异;各组之间受精率及卵裂率总体上无明显差异。
3次重复实验分次结果显示基本一致。FSH组、含药鼠血清1#组均较空白组卵细胞成熟率明显增高,分别P<0.05、P<0.05或P<0.01；含药鼠血清1#组均较空白血清组卵细胞成熟率明显增高,P<0.05;FSH组、含药鼠血清1#组均较空白组GVBD+PBl明显增高,分别P<0.05、P<0.05或P<0.01；FSH与含药鼠血清1#组GVBD+PBl和成熟率比较,无显著性差异。
3.FSH、含药鼠血清1#组CyclinBl的荧光强度较空白组、空白鼠血清组明显增强(15069.00±3908.88、17936.50±4717.42 vs 5029.50±1656.61、6625.33±1777.41,P<0.01);FSH与含药鼠血清1#组比较,无显著性差异。含药鼠血清1#组Cyclin Bl mRNA的表达(Cyclin Bl/GAPDH=2.78)较空白鼠血清组(Cyclin Bl/GAPDH=2)增强。空白组、空白鼠血清组、FSH组、含药鼠血清1#组卵子骨架各组之间比较,无显著性差异。含药鼠血清1#、2#、3#组血清FSH和E2水平均较空白鼠血清组高,无显著性差异。
结论含补肾中药鼠血.清1#体外可以促进小鼠未成熟卵细胞生长发育,主要是促进卵细胞核成熟,并且不增加成熟卵细胞细胞骨架的异常几率。其机制与增强卵细胞成熟促进因子调节亚基Cvclin Bl的表达有关。
Objective To study the effect of traditional chinese medicine for reinforcing the kidney on mouse oocyte in vitro maturation.
Method Inmature oocytes were retreived from 90 ICR murine ovaries stimulated by pregnant mare serum gonadotropin (PMSG).245 mouse cumulus oocyte complexes (COCs) were collected and randomly divided into five groups, rat serum containing traditional chinese medicine 1#,2#,3# for reinforcing the kidney, blank serum(rat serum without traditional chinese medicine) and blank group, and microdrop matured in M199 media for 24 h with 2% rat serum containing traditional chinese medicine 1#,2#,3# for reinforcing the kidney,2% blank serum and medium without rat serum, separately. Oocyte maturation rate were counted. About 238 mouse COCs were collected and randomly divided into four groups, medicated serum 1#, follicle-stimulating hormone (FSH), blank serum and blank group, and matured in media with 2% medicated serum 1#,100IU/L FSH,2% blank serum and medium without rat serum, separately. Oocyte maturation rate, fertilization rate and cleavage rate were counted. The experiment was repeated 3 times. The mouse COCs were collected and randomly divided into four groups, medicated serum 1#, follicle-stimulating hormone (FSH), blank serum and blank group. The expression of oocyte cytoskeleton (spindle and chromosome) and regulatory subunit Cyclin B of mature promoting factor (MPF) were detected by immunofluorescence and observed by reflection fluorescence microscope. And the expression intensity of Cyclin B1 was analyzed by fluorescence testing instrument. The mouse COCs were collected and randomly divided into two groups, medicated serum 1# and blank serum group. Semi-quantity RT-PCR method was used to observe the expression of Cyclin B1 mRNA in mouse oocytes. FSH and E2 in rat blood were determinated by enzyme immunoassay (EIA).
Results
1. The oocyte maturation rate in the medicated serum 1#,2# groups were higher than that in the blank group (42.31%、36.17% vs 16.67%, P<0.01 or P<0.05). The oocyte maturation rate in the medicated serum 1# group was higher than that in the blank serum group (42.31% vs 22.92%, P<0.05). germinal vesicle breakdown and the first polar body (GVBD+PB1) in the medicated serum 1# group was higher than that in the blank group (84.62% vs 66.67%, P<0.05). There was no significant difference between the medicated serum 1#,2#,3# groups.
2. The oocyte maturation rate in the FSH group and the medicated serum 1# group were higher than that in the blank group (33.69%,40.98% vs 17.71%, P<0.01), The oocyte maturation rate in the FSH group and the medicated serum 1# group were higher than that in the blank serum group (33.69%、40.98% vs 22.81%, P <0.05 or P<0.01). GVBD+PB1 in the FSH group and the medicated serum 1# group were higher than that in the blank group (80.75%、83.61% vs 64.00%, P<0.01). GVBD+PB1 in the FSH group and the medicated serum 1# group were higher than that in the blank serum group (80.75%、83.61% vs 71.35%, P<0.05 or P<0.01) There was no significant difference between the FSH group and the medicated serum 1# group.
The experiment was repeated threel times to get the basically consistent outcome. The oocyte maturation rate in the FSH group and the medicated serum 1# group were higher than that in the blank group, respectively, P<0.05, P<0.05 or P<0.01. The oocyte maturation rate in the FSH group and the medicated serum 1" group were higher than that in the blank serum group, P<0.05. GVBD+PB1 in the FSH group and the medicated serum 1# group were higher than that in the blank group, respectively, P<0.05、P<0.05 or P<0.01. There was no significant difference between the FSH group and the medicated serum 1# group.
3. Fluorescence intensity of Cyclin B1 in the FSH group and the medicated serum 1# group were higher than that in the blank group and the blank serum group (15069.00±3908.88,17936.50±4717.42 vs 5029.50±1656.61、6625.33±1777.41, P<0.01).There was no significant difference between the FSH group and the medicated serum 1" group. the expression of Cyclin B1 mRNA in the medicated serum 1# group (Cyclin B1/GAPDH=2.78) were higher than that in the blank serum group (Cyclin B1/GAPDH=2) There was no significant, difference of oocyte cytoskeleton between medicated serum 1", follicle-stimulating hormone (FSH), blank serum and blank group. The levels of FSH and E2 in the medicated serum 1#,2#,3# groups were higher than that in the blank serum group, but there was no significant difference between the four groups.
Conclusion Rat serum containing traditional chinese medicine for reinforcing the kidney 1# has the effect of improving the mouse oocyte development in vitro. The medicated serum 1# has no adverse effect on the cytoskeleton configuration of the mature oocytes. The effect of medicated serum 1" for improving the mouse oocyte in vitro maturation may be ralated to the effect on increasing the expression of maturation promoting factor regulatory subunit Cyclin B1.
引文
1王维华,孙青原主编.生育革命迎接试管婴儿新时代[M].北京：科学出版社,2007：188
2张树成,沈明,蔡连香,等.补肾调经和养血补肾方药调经、促排卵作用的实验研究[J].中医杂志,1999,40(6)：369-371
3连方,滕依丽,张建伟,等.二至天癸颗粒对体外受精-胚胎移植周期人卵泡液白血病抑制因子和卵细胞质量的影响[J].中国中西医结合杂志,2007,27(11)：976-979
4连方,孙振高,张建伟,等.二至天癸方对小鼠卵细胞质量影响的实验研究[J].中国中西医结合杂志,2004,24(7)：625-6275
5杨桂云,王佩娟,贾晓斌,等.补肾活血汤对小鼠体外受精及其早期胚胎发育的影响[J].中国中西医结合杂志,2001,21(7)：522-524
6丁原全,万绍晖,南春红,等.补肾复方对围绝经期大鼠卵巢VEGF影响的实验研究[J].中医药学刊,2006,24(4)：676-677
7顿文亮,方泰惠,张丽娟,等.补肾助孕方对LPD大鼠卵巢中VEGF蛋白表达的影响[J].中国实用医药,2008,3(25)：54-55
8刘艳巧,党慧敏,刘润侠,等.助孕胶囊对多囊卵巢综合征大鼠卵巢局部细胞因子表达的影响[J].中医杂志,2008,49(5)：453-455,458
9黄琼霞,周安方,曹继刚,等.补肾毓麟汤对肾虚性生殖功能障碍大鼠血清EGF及生殖器官EGFR表达影响的实验研究[J].湖北中医杂志,2007,29(9)：3-4
10连方,孙振高,穆琳,等.二至天癸颗粒提高卵细胞质量与小鼠卵巢内IGF-1R mRNA表达量关系的研究[J].中国中西医结合杂志,2006,26(5)：431-434
11陈怡,孙慧兰.固肾安胎中药对小鼠早期胚胎体外发育的影响[J].中国中药杂 志,2008,33(14)：1767-1768
12张树成,陈秋梅,夏誉薇,等.补肾助孕方药促进人子宫内膜血管生成的实验研究[J].中医药学刊,2004,22(5)：820-822
13吴云霞,黄光英.补肾益气活血方对吲哚美辛致胚泡着床障碍模型小鼠胚泡着床的影响[J].中国医学研究与临床,2005,4(3)：1-4
14王素霞,孙玉英.安胎合剂对GnRHa超排卵小鼠子宫内膜形态学的影响[J].中医药学报,2006,34(5)：45-47
15张明敏,黄玉琴.补肾安胎方改善胚泡着床障碍小鼠局部血管形成及其机制[J].华中科技大学学报(医学版),2006,35(6)：781-783
16周珍辉主编.动物细胞培养技术[M].中国环境科学出版社,2006：250
17 Mao J, Wu G, Smith MF, et al. Effects of culture medium, serum type, and various concentrations of follicle-stimulating hormone on porcine preantral follicular development and antrum formation in vitro[J]. Biol Reprod.2002,67(4):1197-203
18 Schoevers EJ, Kidson A, Verheijden JH, et al. Effect of follicle-stimulating hormone on nuclear and cytoplasmic maturation of sow oocytes in vitro[J]. Theriogenology.2003,59(9):2017-2028
19 Atef A, Francois P, Christian V, et al. The potential role of gap junction communication between cumulus cells and bovine oocytes during in vitro maturation[J]. Mol Reprod Dev.2005,71(3):358-367
20 Coticchio G, Rossi G, Borini A, et al. Mouse oocyte meiotic resumption and polar body extrusion in vitro are differentially influenced by FSH, epidermal growth factor and meiosis-activating sterol[J]. Hum Reprod, 2004,19(12):2913-2918
21 Yang SH, Son WY, Yoon SH, et al. Correlation between in vitro maturation and expression of LH receptor in cumulus cells of the oocytes collected from PCOS patients in HCG-primed IVM cycles[J]. Hum Reprod, 2005,20(8):2097-2103
22 Choi YH, Carnevale EM, Seidel GE, et al. Effects of gonadotropins on bovine oocytes mature in TCM-199[J]. Theriogenology,2001,56(4):661-670
23 Junk SM, Dharmarajan A, Yovich JL. FSH priming improves oocyte maturation, but priming with FSH or hCG has no effect on subsequent embryonic development in an in vitro maturation program[J]. Theriogenology,2003,59(8):1741-1749
24 Javed A, Jamil A, Rezaei-Zarchi S, et al. An in vitro comparative study of follicle stimulating hormone (FSH) and activin A effects on the maturation of preantral follicle-enclosed oocytes from immature Syrian mice[J]. Iran Biomed J,2008,12(2):85-92
25石少权,苏兆娟,丘彦.卵泡刺激素和卵丘细胞对小鼠卵母细胞体外成熟的影响[J].实用诊断与治疗杂志,2005,19(8)：549-551
26陈勇,夏国良,苏友强,等.体外培养条件下促性腺激素对昆明白小鼠卵母细胞成熟及卵丘扩展的影响[J].动物学报,2001,47(2)：203-208
27 Guler A, Poulin N, Mermillod P, et al. Effect of growth factors, EGF and IGF—I, and estradiol on in vitro maturation of sheep ooeytes[J]. Theriogenology,2000,54 (2):209-218
28 Kim MK, FibriantoYH, Oh H J, et al. Effects of estradiol-17beta and porgesterone supplementation on in vitro nuclear maturation of canine oocytes[J]. Theriogenology,2005,63 (5):1342-1353
29 Ali A, Sirard MA. The effect of 17-beta estradiol and protein supplement on the response to purified and recombinant follicle stimulating hormone in bovine oocyte[J]. Zygote,2002,10 (1):65-71
30 Zheng P, Si W, Bavister B, et al.17β-estradiol and progesterone improve in-vitro cytoplasmic maturation of oocytes from unstimulated prepubertal and adult rhesus monkeys[J]. Hum Reprod,2003,18(10):2137-2144
31陈媛,杨菁,李洁,等.促性腺激素及雌二醇对小鼠卵母细胞体外成熟的影响[J].武汉大学学报(医学版),2007,28(5)：568-572,587
32 Beker AR, Colenbrander B, Bevers MM. Effect of 17beta-estradiol on the in vitro maturation of bovine oocyte [J]. Theriognology,2002,58:1663-1673
33 Dode MA, Graves CN. Role of estradiol-17beta on nuclear and cytoplasmic maturation of pig oocytes[J]. Anim Reprod Sci,2003,78(1/2):99
34李江源主编.性腺疾病[M].天津科技翻译出版公司,1997：58
35 Gupta PS, Nandi S, Ravindranatha BM, et al. Effect of commercially available PMSG on maturation, fertilization and embryo development of buffalo oocytes in vitro[J]. Reprod Fertil Dev,2001,13(5-6):355-360
36王坤,林涛,赵明辉,等.孕马血清促性腺激素对狗卵母细胞体外核成熟的影响[J].安徽农业科学,2008,36(35)：15503-15504,15509
37 Boiso I, Marti M, Santalo J, et al.A confocal microscopy analysis of the spindle and chromosome configurations of human oocytes cryopreserved at the germinal vesicle and metaphase Ⅱ stage[J]. Hum Reprod.2002 Jul;17(7):1885-1891
38王晓梅,黄天华,谢庆东,等Dynein抑制剂对卵母细胞体外成熟及Cyclin B1 mRNA水平的影响[J].癌变·畸变·突变,2004,16(5)：267-271
39王晓梅,鞠桂芝,傅海青,等.4Gy X射线照射对EL-4细胞Cyclin B1及cdc2 mRNA水平的影响[J].辐射研究与辐射工艺学报,2004,22(1)：43-46
40 杨菁. 体外受精与胚胎移植技术临床进展http://dryang.jing. haodf. com/wenzhang/103348. htm,2009.12.1
41胡建华,姚明,崔淑芳.实验动物学教程[M].上海科学技术出版社,2009：568
42陈大元.受精生物学受精机制与生殖工程[M].科学出版社,2000：146
43许美解,李刚主编.动物繁殖技术[M].化学工业出版社,2009：50
44 Peter R. Brinsden原著.体外受精与辅助生殖Bourn Hall诊所临床实践与实验室操作指南第3版[M].人民卫生出版社,2009：342
45王心如,周作民.生殖医学[M].人民卫生出版社,2004：38-39
46杨利国.动物繁殖学[M].中国农业出版社,2003：157-158
47 Coleman T R, Dunphy WG. Cdc2 regulatury factors[J]. Curr Opin Cell Biol,1994,6 (6):877-882
48 Yoshida N, Mita K, Yamashita M. Comparative study of the moleculer mechanism of oocyte maturation in amphibians[J]. Comp Bioehem Physiol B Biochem Mol Biol,2000,126(2):189-197
49 Shimada M, Zeng W X, Terada T. Inhibition of phosphatidyli-nositol 3-kinase of mitogen-activated protein kinase leads to suppression of p34(cdc2)kinase activity and meiotic progression beyond the meiosis I stage in porcine oocytes surrounded with cumulus cells[J]. Biol Reprod,2001,65(2):442-448
50夏蕾,张岭.卵母细胞成熟过程MPF的调控和MPF的作用底物[J].疾病控制杂志,2007,11(6)：614-617
51詹启敏.分子肿瘤学[M].人民卫生出版社,2005：95
52桑润滋.动物繁殖生物技术[M].中国农业出版社,2002：25
53 Sagata N. Meiotic metaphase arrest in animal oocytes:its mechanisms and biological significance[J]. Trends Cell Biol,1996,6(1):22-28
54 Sun QY, Lai L, Park KW, et al. Dynamic events are differently mediated by microfilaments, microtubules, and mitogen-activated protein kinase during porcine oocyte maturation and fertilization in vitro[J]. Biol Reprod,2001,64(3):879-889
55 Wang Q, Sun QY. Evaluation of oocyte quality:Morphological, cellular and molecular predictors[J]. Reprod Fertil Dev,2007,19(1):1-12
56 Wang WH, Keefe DL. Prediction of chromosome misalignment among in vitro mature human oocytes by spindle imaging with the PolScope [J]. Fertil Steril,2002,78(5):1077-1081
57 Coticchio G, Sereni E, Serrao L, et al. What criteria for the definition of oocyte quality?[J]. Ann N Y Acad Sci,2004,1034:132-144
58 Wang WH, Meng L, Hackett RJ, et al. Limited recovery of meiotic spindles in living human oocytes after cooling-rewarming observed using polarized light microscopy [J]. Hum Reprod,2001,16(11):2374-2378
59崔龙波,周雪莹,崔志峰,等.不同因素对小鼠MⅡ期卵母细胞纺锤体的影响[J].安徽农业科学,2008,36(33)：14555-14557
60崔奎青,石德帧,杨素芳.兔卵母细胞去核方法的研究[J].中国畜牧兽医,2005,32(7)：32-34
61 Combelles CM, Cekleniak NA, Racowsky C, et al. Assessment of nuclear and cytoplasmic maturation in in-vitro mature human oocytes [J]. Hum Reprod,2002,17(4):1006-1016
62朱亮,陈士岭,邢福祺,等.培养环境的温度变化与人MⅡ期卵母细胞纺锤体形态学变化的关系[J].第四军医大学学报,2005,26(15)：1414—1416
63 Aman RR, Parks JE. Effects of cooling and rewarming on the meiotic spindle and chromosomes of in vitro-mature bovine oocytes[J]. Biol Reprod,1994,50(1):103-110
64张文红,孙筱放,陈欣洁,等.不同温度对小鼠卵母细胞纺锤体的影响[J].基础医学与临床,2005,25(8)：721-724
65杨美娟.组织标本固定方法与免疫组织化学染色效果的关系[J].解剖科学进展,2003,9(1)：89-90
66 Li Y, Feng HL, Cao YJ, et al. Confocal microscopic analysis of the spindle and chromosome configurations of human oocytes mature in vitro [J]. Fertil Steril,2006,85(4):827-832
67 Moon JH, Jee BC, Ku SY, et al. Spindle positions and their distributions in in vivo and in vitro mature mouse oocytes[J]. Hum Reprod,2005,20(8):2207-2210
68段恒.资冲颗粒灌胃和直肠给药促卵泡发育的实验研究[D].成都中医药大学,2003
69冯雪,周惠芳.卵泡发育不良综合征的研究进展[J].长春中医药大学学报,2009,25(2)：299-300
70连方,王玉东,张宁.中医药补肾促卵泡发育的研究进展[J].中国中西医结合杂志,2000,20(4)：313-314,317
71马景兰,马建新,王旭初,等.促孕丸在“试管婴儿”中的应用[J].中国中西医结合杂志,2005,25(6)：547-548
72黄舒娥.中药人工周期疗法配合辅助生育技术对体外受精胚胎种植率的影响[J].广州中医药大学学报,2006,23(4)：302-204
73谈勇,石川睦男.补肾调周法在体外授精-胚胎移植前应用的临床观察[J].中国中医药信息杂志,2001,8(12)：56-57
74连方,滕依丽,张建伟,等.二至天癸颗粒联合体外受精-胚胎移植治疗不孕症61例临床研究[J].中医杂志,2006,47(6)：439-441
75李东.补肾养血活血法联合体外受精-胚胎移植对妊娠率的影响[J].中医杂志,2008,49(12)：1084-1086
76许小凤,谈勇,陈秀玲,等.补肾活血中药对卵巢储备功能的影响[J].江苏中医药,2007,39(2)：18-21
77李东,郭佳.补肾调周法改善卵巢储备功能在辅助生殖技术中运用的临床研究[J].北京中医药大学学报,2008,31(2)：131-134
78徐珉,司徒仪,李丽芸,等.中医补肾法对高龄不孕妇女的卵巢储备功能的调控[J].广州中医药大学学报,2004,21(4)：267-271
79单志群,曾勇,胡晓东,等.补肾调冲法在试管婴儿助孕技术中的运用——附96例临床报告[J].中医药学报,2002,30(6)：10-11
80王宁生,雷燕,刘平,等.关于血清药理学的若干思考[J].中国中西医结合杂志,1999,19(5)：263
81李军昌,夏天,黄熙,等.中药复方药理的研究工具——血清药理学[J].中药药理与临床,1998,14(6)：44
82詹红生.含药血清实验方法及其在中药新药研制中的应用进展[J].浙江中医学院学报,2000,24(2)：70-81
83 Iwama H, Amagaya S, Ogihara Y, et al. Effect of shosaikoto,a Japanese and Chinese traditional herbal medical mixture, on the mitogentic activity of lipopolysaccharide:a new pharmacological testing method [J]. J Ethoharmacol,1987,21(1):45-53
84吴健宇,穆静,李仪奎.血清药理实验中异种动物血清对细胞的毒性作用和灭活后的减毒作用[J].中国药理学通报,2000,16(1)：118-119
1张丽珠.临床生殖内分泌与不育症第2版[M].北京：科学出版社,2006：491-576
2 Linda MacDonald Glenn,An Economi st Looks at the Fertility Industry,Oct 01 2008, available at http://stanford. well sphere, com/bioethics-article/an-economist-looks-at-the-fert ility-industry/403947
3王维华,孙青原主编.生育革命迎接试管婴儿新时代[M].北京：科学出版社,2007：188
4张树成,沈明,蔡连香,等.补肾调经和养血补肾方药调经、促排卵作用的实验研究[J].中医杂志,1999,40(6)：369-371
5连方,滕依丽,张建伟,等.二至天癸颗粒对体外受精-胚胎移植周期人卵泡液白血病抑制因子和卵细胞质量的影响[J].中国中西医结合杂志,2007,27(11)：976-979
6连方,孙振高,张建伟,等.二至天癸方对小鼠卵细胞质量影响的实验研究[J].中国中西医结合杂志,2004,24(7)：625-627
7王素霞,孙玉英.安胎合剂对GnRHa超排卵小鼠子宫内膜形态学的影响[J].中医药学报,2006,34(5)：45-47
8黄冬梅,黄光英,陆付耳.补肾益气和血方中药对胚泡着床障碍小鼠子宫内膜表面胞饮突表达的影响[J].中华妇产科杂志,2004,39(4)：230-233
9杨桂云,王佩娟,贾晓斌,等.补肾活血汤对小鼠体外受精及其早期胚胎发育的影响[J].中国中西医结合杂志,2001,21(7)：522-524
10 Sharon LaMothe, Birth of Octoplets Raises Questions About Fertility Industry, May 04 2009, available at http://stanford, wellsphere. com/pregnancy-fertility-article/birth-of-octoplets-r aises-questions-about-fertility-industry/662003
11 Foulot H, Ranoux C, Dubuisson JB, et al. In vitro fertilization without ovarian stimulation:a simplified protocol applied in 80 cycles [J]. Fertil Steril,1989,52 (4):617-621
12 Janssens RMJ, Lambalk CB, Vermeiden JPW, et al. Invitro fertilization in a spontaneous cycle:easy, cheap and realistic[J]. Hum Reprod,2000,15(2):314-318
13 D'Amato G, Caroppo E, Pasquadibisceglie A, et al. A novel protocol of ovulation induction with delayed gonadotropin-releasing hormone antagonist administration combined with high-dose recombinant follicle-stimulating hormone and clomiphene citrate for poor responders and women over 35[J]. Fertil Steril,2004,81(6):1572-1577
14 Williams SC, Gibbons WE, Muasher SJ, et al. Minimal ovarian hyperstimulation for in vitro fertilization using sequential clomiphene citrate and gonadotropin with or without the addition of a gonadotropin-releasing hormone antagonist [J]. Fertil Steril,2002,78(5):1068-1072
15 Weigert M, Krischker U, Pohl M, et al. Comparison of stimulation with clomiphene citrate in combination with recombinant follicle stimulation with a gonadotropin-releasing hormone agonist protocol:a prospective, randomized study [J]. Fertil Steril,2002,78(1):34-39
16 Engel JB, Ludwig M, Felberbaum R, et al. Use of cetrorelix in combination with clomiphene citrate and gonadotropins:a suitable approach to" friendly IVF" [J]. Hum Reprod,2002,17(8):2 022-2026
17 Hwang JL, Huang LW, Hsieh BC, et al. Ovarian stimulation by clomiphene citrate and hMG in combination with centrorelix acetate for ICSI cycles[J]. Hum Reprod,2003,18(1):45-49
18 Palermo G, Joris H, Devroey P, et al. Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte[J].1992,340(8810):17-18
19李蓉,庄广伦,张敏芳.卵母细胞单精子显微注射治疗男性因素及不明原因小育[J].中华妇产科杂志,1997,32(4)：211-213
20 Handyside AH, Scriven PN, Ogilvie CM. The future of preimplantation genetic diagnosis[J]. Hum Reprod,1998,13 Suppl 4:249-255
21广东中山大学附属第一医院庄广伦教授日前指出——我国PGD技术应用要树立中国特色http://www. cnpharm. com/paperpdf/2005/Yy5-0820. pdf,2005.8.20
22 乔杰,张丽珠.中国大陆辅助生殖技术现状、挑战和发展http://byby.39.net/103/1/1167267, html,2010.3.1
23 Cha KY, Koo JJ, Ko JJ, et al. Pregnancy after in vitro fertilization of human follicular oocytes collected from nonstimulated cycles, their culture in vitro and their transfer in a donor oocyte program[J]. Fertil Steril,1991,55(1):109-113
24 乔 杰 . 试 管 婴 儿 新 技 术 须 谨 慎 推广http://www. sciencenet. cn/htmlnews/20079189538935189865.html,2007.9.18
25 Geoffrey Planer, World's first'ice baby'-25th birthday,30 March 2009, available at http://www. ivf. net/ivf/world_s_first_ice_baby_25th_birthday-o4078. html
26中国科学技术协会.中国科学技术专家传略医学编临床医学卷3[M].人民卫生出版社,2007：66
27 中国首名冷冻卵子技术培育的试管婴儿已问世 http://www. chinanews. com. cn/n/2004-05-11/26/434782. shtml,2004.5.11
28 国 内 首 例 “ 三 冻 ” 试 管 婴 儿 在 京 诞生http://news. xinhuanet. com/health/2006-02/10/content_4162145. htm,2006.2.10
29 Thomson JA, Itskovitz-Eldor J, Shapiro SS, et al. Embryonic stem cell lines derived from human blastocysts[J]. Science,1998,282(5391):1145-1147
30 Shamblott MJ, Axelman J, Wang S, et al. Derivation of pluripotent stem cells from cultured human primordial germ cell [J]. Proc Natl Acad Sci USA,1998,95(23):13726-13731
31 Chen Y, He ZX, Liu A, et al. Embryonic stem cells generated by nuclear transfer of human somatic nuclei into rabbit oocytes [J]. Cell Res,2003,13(4)-.251-263
32 Hubner K, Fuhrmann G, Christenson LK, et al. Derivation of oocytes from mouse embryonic stem cells [J]. Science,2003,300(5623):1251-1256
33 Vogel G. Embryonic stem cells. Scientists make sperm in a dish[J]. Science,2003,302 (5652):1875
34 Zhang J, Wang CW, Krey L, et al. In vitro maturation of human preovulatory oocytes reconstructed by germinal vesicle transfer[J]. Fertil Steril,1999,71(4):726-731
35 中 国 第 四 代试管婴 儿武汉 出 生 将 是 唯 一 一例http://www. chinanews. com. cn/n/2004-02-25/26/406288. html,2004.2.25
36乔杰.人类辅助生殖技术的新进展[J].中国实用妇科与产科杂志,2008,24(1)：33-34
37王克华,李娟,张琦等.卵子赠送技术的应用及进展[J].国外医学计划生育分朋,2003,22(2)：94-96
38张松英主编.妇产科疾病临床治疗与合理用药[M].科学技术文献出版社,2007：344
39 Movahedin M, Ajeen A, Ghorbanzadeh N, et al. In vitro maturation of fresh and frozen-thawed mouse round spermatids[J]. Andrologia,2004,36(5):269-276
40 Badawy A, Elnashar A, Totongy M. Clomiphene citrate or aromatase inhibitors for superovulation in women with unexplained infertility undergoing intrauterine insemination:a prospective randomized trial[J]. Fertil Steril,2009,92(4):1355-1359
41 Patchava S, Gelbaya TA, Nardo LG. Managing the complications associated with assisted reproductive technologies[J]. Minerva Ginecol,2009,61 (4):347-355
42王芳,孙莹璞,孔慧娟,等.不同辅助生殖技术妊娠分娩的1540例新生儿出生缺陷分析[J].中华妇产科杂志,2009,44(2)：108-111
43 Multiple gestation pregnancy. The ESHRE Capri Workshop Group[J]. Hum Reprod,2000,15(8):1856-1864
44 Schieve LA. Multiple-gestation pregnancies after assisted reproductive technology treatment:population trends and future directions[J]. Women's Health, 2007,3 (3):301-307
45 Schnorr JA, Doviak MJ, Muasher SJ, et al. Impact of a cryop reservation p rogram on the multiple pregnancy rates associated with assisted reproductive technologies[J]. Fertil Steril,2001,75(1):147-151
46钟刚,蔡桂茹.助孕技术对多胎妊娠发生率的影响[J].国外医学.妇产科学分册,1999,26(2)：112
47 Shulman A, Ghetler Y, Beyth Y, et al. The significance of an early (premature) rise of plasma progesterone in in vitro fertilization cycles induced by a "long protocol" of gonadotropin releasing hormone analogue and human menopausal gonadotropins[J]. J Assist Reprod Genet,1996,13(3):207-211
48 Ertzeid G, Storeng R. The impact of ovarian stimulation on implantation and fetal development in mice[J]. Hum Reprod,2001,16(2):221-225
49 Shi W, Haaf T. Aberrant methylation patterns at the two-cell stage as an indicator of early development failure[J]. Mol Reprod Dev,2002,63(3):329-334
50 Chang AS, Moley KH, Wangler M, et al. Association between Beckwith-Wiedemann syndrome and assisted reproductive technology:a case series of 19 patients[J]. Fertil Steril,2005,83(2):349-354
51 Shevell T, Malone FD, Vidaver J, et al. Assisted reproductive technology and pregnancy outcome[J]. Obstet Gynecol,2005,106(5 Pt 1):1039-1045
52 Bohlmann MK, Fritzsching B, Luedders DW, et al. Impact of assisted reproduction on obstetrics and neonatology[J]. Z Geburtshilfe Neonatol,2009,213(6):221-227
53 Allen C, Bowdin S, Harrison RF, et al. Pregnancy and perinatal outcomes after assisted reproduction:a comparative study[J]. Ir J Med Sci,2008,177(3):233-241
54 Wang JX, Norman RJ, Wilcox AJ. Incidence of spontaneous abortion among pregnancies produced by assisted reproductive technology[J]. Hum Reprod,2004,19(2):272-277
55 Wang YA, Sullivan EA, Black D, et al. Preterm birth and low birth weight after assisted reproductive technology-related pregnancy in Australia between 1996 and 2000[J]. Fertil Steril.2005,83(6):1650-1658
56 Jackson RA, Gibson KA, Wu YW, et al. Perinatal outcomes in singletons following in vitro fertilization:a meta-analysis[J], Obstet Gynecol,2004,103(3):551-563
57 Koivurova S, Hartikainen AL, Gissler M, et al. Neonatal outcome and congenital malformations in children born after in-vitro fertilization[J]. Hum Reprod, 2002,17(5):1391-1398
58 Schieve LA, Meikle SF, Ferre C, et al. Low and very low birth weight in infants conceived with use of assisted reproductive technology[J]. N Engl J Med,2002,346(10):731-737
59 Strandell A, Thorburn J, HambergerL. Risk factors for ectop ic p regnancy in assisted rep roduction[J]. Fertil Steril JT Fertility and Sterility,1999,71 (2):282-286
60 Alsunaidi M. Incidence of ectopic pregnancy after assisted reproduction treatment[J].2007,28 (4):590-592
61 Rossing MA, Tang MT, Flagg EW, et al. A case control study of ovarian cancer in relation to infertility and the use of ovulation inducing drugs [J]. Am J Epidemiol,2004,160(11):1070-1078
62 Halliday JL, Ukoumunne OC, Baker HW, et al. Increased risk of blastogenesis birth defects, arising in the first 4 weeks of pregnancy, after assisted reproductive technologies[J]. Hum Reprod,2010,25(1):59-65
63 Reefhuis J, Honein MA, Schieve LA, et al. Assisted reproductive technology and major structural birth defects in the United States[J]. Hum Reprod,2009,24 (2):360-366
64 Kallen B, Finnstrom 0, Nygren KG, et al. In vitro fertilization(IVF) in Sweden:risk for congenital malformations after different IVF methods[J]. Birth Defects Res A Clin Mol Teratol,2005,73(2):162-169
65 Hansen M, Kurinczuk JJ, Bower C, et al. The risk of major birth defects after intracytoplasmic sperm injection and in vitro fertilization[J]. N Engl J Med,2002,346:725-730
66 Kuwata T, Matsubara S, Ohkuchi A, et al. The risk of birth defects in dichorionic twins conceived by assisted rep roductive technology [J]. Twin Res,2004,7 (3):223-227
67连方,明蔚.体外授精2胚胎移植患者降调节后肾虚本质探讨[J].山东中医药大学学报,2008,32(2)：109-110
68 Coyle M, Smith C. A survey comparing TCM diagnosis, health status and medical diagnosis in women undergoing assisted reproduction[J]. Acupunct Med, 2005,23 (2):62-69
69李东.温肾安胎饮联合辅助生殖技术提高胚胎移植失败患者妊娠率的临床研究[J].北京中医药大学学报,2009,32(2)：139-141
70张建伟.体外受精-胚胎移植降调节后中医辨证思路与方法[J].中医药通报,2007,6(3)：36-37
71连方,滕依丽,张建伟,等.二至天癸颗粒联合体外受精-胚胎移植治疗不孕症61例临床研究[J].中医杂志,2006,47(6)：439-441
72连方,滕依丽,张建伟,等.二至天癸颗粒对体外受精-胚胎移植周期人卵泡液白血病抑制因子和卵细胞质量的影响[J].中国中西医结合杂志,2007,27(11)：976-979
73许小凤,谈勇,陈秀玲,等.补肾活血中药对卵巢储备功能的影响[J].江苏中医药,2007,39(2)：18-21
74李东.补肾养血活血法联合体外受精-胚胎移植对妊娠率的影响[J].中医杂志,2008,49(12)：1084-1086
75许娟,冯玉华,汤丽莎,等.补肾活血方对促排卵中子宫内膜发育不良的影响[J].江西中医药,2007,38(5)：42-43
76单志群,曾勇,胡晓东,等.补肾调冲法在试管婴儿助孕技术中的运用——附96例临床报告[J].中医药学报,2002,30(6)：1011
77吴育宁.中医药辅助试管婴儿的治疗经验[J].中国中西医结合杂志,2007,27(3)：270-271
78李东,郭佳.补肾调周法改善卵巢储备功能在辅助生殖技术中运用的临床研究[J].北京中医药大学学报,2008,31(2)：131-134
79黄舒娥.中药人工周期疗法配合辅助生育技术对体外受精胚胎种植率的影响[J].广州中医药大学学报,2006,23(4)：302-204
80谈勇,石川睦男.补肾调周法在体外授精-胚胎移植前应用的临床观察[J].中国中医药信息杂志,2001,8(12)：56-57
81金保方,杨晓玉,张新东,等.中医治疗反复不明原因IVF失败后成功妊娠52例[J].南京中医药大学学报,2009,25(1)：56-58
82徐珉,司徒仪,李丽芸,等.中医补肾法对高龄不孕妇女的卵巢储备功能的调控[J].广州中医药大学学报,2004,21(4)：267-271
83连方,张建伟,张宁,等.中西医结合疗法在试管婴儿技术中的应用[J].山东中医药大学学报,2002,26(3)：182-183
84连方,张宁,张建伟,等.中药配合体外授精及胚胎移植治疗不孕症2例[J].中国中西医结合杂志,2002,22(8)：602
85姜朵生,丁玎.针药治疗持续不排卵性不孕症疗效观察[J].中国针灸,2009,29(1)：21-24
86姜萍,傅萍.傅萍运用养精种玉汤治疗试管婴儿术前后临床经验[J].浙江中医杂志,2009,44(3)：170-171
87张明敏,黄光英,陆付耳,等.补肾益气活血汤对多次助孕技术失败患者结局的影响[J].微循环学,2002,12(1)：10-13
88张明敏,黄光英,陆付耳,等.银杏叶制剂对体外授精治疗中卵巢反应低下患者的作用[J].中国中西医结合杂志,2003,23(3)：171-173
89马景兰,马建新,王旭初,等.促孕丸在“试管婴儿”中的应用[J].中国中西医结合杂志,2005,25(6)：547-548
90朱文杰,李雪梅,陈秀敏,等.滋肾育胎丸对体外受精-胚胎移植患者胚胎种植率的影响[J].中国中西医结合杂志,2002,22(10)：729-731
91徐国男.针灸加中药治疗对提高试管婴儿成功率的疗效观察[J].天津中医药,2006,23(4)：341-342
92陈海燕,夏国守.体外受精和胚胎移植技术中应用中医药对男性不育患者治疗结果影响的研究[J].现代中西医结合杂志,2005,14(9)：1240-1241
93金保方,黄宇烽,杨晓玉,等.养精胶囊治疗弱精子症的临床观察[J].南京中医药大学学报,2006,22(5)：286-289
94杨明,王树声,陈志强,等.中药对肾阳虚不育症患者精子DNA的影响[J].现代中西医结合杂志,2002,11(10)：889
95翟亚春.男子免疫性不育症辨治思路初探[J].江苏中医,2000,21(11)：15
96倪江,张威,边淑玲,等.消抗安胎液对男性血清抗精子抗体及白细胞介素2受体水平的影响[J].中医药学报,1999,4：49
97 Zhang M, Huang G, Lu F, et al. Influence of acupuncture on idiopathic male infertility in assisted reproductive technology [J]. J Huazhong Univ Sci Technolog Med Sci,2002,22(3):228-230
98 Stener-Victorin E, Humaidan P. Use of acupuncture in female infertility and a summary of recent acupuncture studies related to embryo transfer[J]. Acupunct Med,2006,24(4):157-163
99 Cai X. Substitution of acupuncture for human chorionic gonadotropin in ovulation induction[J]. J Tradit Chin Med,1997,17(2):119
100 Stener-Victorin E, Waldenst rom U, Tagnfors U, et al. Effect s of electro-acupuncture on anovulation in women with polycystic ovary syndrome[J]. Acta Obstet Gynecol Scand,2000,79:180
101 Magarelli PC, Cridennda DK, Cohen M. Changes in serum cortisol and prolactin associated with acupuncture during controlled ovarian hyperstimulation in women undergoing in vitro fertilization-embryo transfer treatment[J]. Fertil Steril,2009,92(6):1870-1879
102桑海莉.补肾加针刺治疗多囊卵巢综合征35例临床观察[J].中医杂志,2000,41(7)：412
103俞瑾.多囊卵巢综合征的中西医治疗[J].中国实用妇科与产科杂志,2002,18(11)：651
104杨越红,洪建云,魏达友,等.针刺治疗多囊卵巢综合征所致不孕的临床观察[J].广东医学院学报,2005,23(4)：377
105陈栋,陈恕仁,石晓兰,等.针挑治疗多囊卵巢综合征临床观察[J].中国针灸,2007,27(2)：9-11
106宋丰军,郑士立,马大正.针灸治疗排卵障碍性不孕症临床观察[J].中国针灸,2008,28(1)：21-23
107连方,李海仙,张建伟,等.电针促使LUFS病人排卵的临床研究[J].上海针灸杂志,2006,25(8)：3-4
108董纪翠.电针治疗卵泡发育不良临床观察[J].中国中医药信息杂志,2007,14(9)：68-69
109陈军,刘莉莉,崔薇,等.电针干预对卵巢低反应患者体外受精胚胎移植的影响[J].中国针灸,2009,29(10)：775-779
110 Stener-Victorin E, Waldenstrom U, Andersson SA, et al. Reduction of blood flow impedance in the uterine arteries of infertile women with elect ro-acupuncture[J]. Hum Reprod,1996,11:1314
111 Manheimer E, Zhang G, Udoff L, et al. Effects of acupuncture on rates of pregnancy and live birth among women undergoing in vitro fertilisation:systematic review and meta-analysis[J]. BMJ,2008,336(7643):545-549
112 Cheong YC, Hung Yu Ng E, Ledger WL. Acupuncture and assisted conception[J]. Cochrane Database Syst Rev,2008,8(4):CD006920
113 Paulus WE, Zhang MM, Strehler Erwin, et al. Influence of acupuncture on the pregnancy rate in patients who undergo assisted reproduction therapy[J]. Fertil Steril,2002,77:721-724
114张明敏,黄光英,陆付耳,等.针刺对胚胎移植怀孕率的影响及其机理：随机安慰对照研究 [J].中国针灸,2003,23(1)：3-5
115 Westergaard LG, Mao Q, Krogslund M, et al. Acupuncture on the day of embryo transfer significantly improves the reproductive outcome in infertile women:a prospective, randomized trial[J]. Fertil Steril,2006,85(5):1341-1346
116 Johnson D. Acupuncture prior to and at embryo transfer in an assisted conception unit—a case series [J]. Acupunct Med,2006,24(1):23-28
117 Smith C, Coyle M, Norman RJ. Influence of acupuncture stimulation on pregnancy rates for women undergoing embryo transfer [J]. Fertil Steril,2006,85(5):1352-1358
118 Dieterle S, Ying G, Hatzmann W, et al. Effect of acupuncture on the outcome of in vitro fertilization and intracytoplasmic sperm injection:a randomized, prospective, controlled clinical study[J]. Fertil Steril,2006,85(5):1347-1351
119崔薇,孙伟,刘莉莉,等.电针干预对体外受精-胚胎移植患者的作用研究[J].中国妇幼保健,2007,22(24)：3403-3405
120崔薇,刘莉莉,孙伟,等.电针对不同证型不孕症患者体外受精-胚胎移植作用的研究[J].中国针灸,2008,28(4)：254-256
121谢广妹.菟丝子水提取物治疗卵巢过度刺激综合征实验研究[J].兰州大学学报(医学版),2009,35(3)：4-26,31
122林丽波,姚美玉,吴效科,等.卵巢过度刺激综合征的中医治疗概述[J].世界中西医结合杂志,2009,4(3)：255256
123辛明蔚.连方预防卵巢过度刺激综合征的经验[J].山东中医药大学学报,2007,31(6)：476-477
124刘凤云,张敏,吴先哲.中医药治疗卵巢过度刺激综合征的思路与方法[J].中医杂志,2004,45(2)：143-144
125俞而慨,归绥琪,邹琴娣.中西医结合治疗中重度卵巢过度刺激综合征的临床观察[J].中国中西医结合杂志,2003,23(3)：175-177
126孙永忠,费小阳,沈云华,等.中西医结合治疗卵巢过度刺激综合征临床观察[J].实用中医药杂志,2007,23(10)：660-661
127刘颖,刘云鹏.中西医结合治疗卵巢过度刺激综合征体会[J].中国中医药信息杂志,2004,11(11)：1011-1012
128林敏.中西医结合治疗卵巢过度刺激综合征[J].四川中医,2003,21(4)：58-59
129孙伟,张敏.中西医结合治疗重度卵巢过度刺激综合征[J].现代中西医结合杂志,2009,18(26)：3211,3249
130张晓红,杨正望,尤昭玲.补肾中药对卵巢功能及子宫内膜的影响[J].中医药学刊,2005,23(11)：2054-2055
131 Huang ST, Chen AP. Traditional Chinese medicine and infertility[J]. Curr Opin Obstet Gynecol,2008,20(3):211-215
132 Huang BM, Hsiao KY, Chuang PC, et al. Upregulation of steroidogenic enzymes and ovarian 17beta-estradiol in human granulosa-lutein cells by Cordyceps sinensis mycelium[J]. Biol Reprod,2004,70(5):1358-1364
133连方,孙振高,张建伟,等.二至天癸方对小鼠卵细胞质量影响的实验研究[J].中国中西医结合杂志,2004,24(7)：625-627
134连方,孙振高,穆琳,等.二至天癸颗粒提高卵细胞质量与小鼠卵巢内IGF-1R mRNA表达量关系的研究[J].中国中西医结合杂志,2006,26(5)：431-434
135戴锦成,周小平.宝生汤对生精障碍小鼠睾丸及精子的影响[J].福建中医学院学报,2002,12(4)：34
136江峰,王益鑫,盛新福,等.体外添加黄芪对精子质量的影响[J].男性学杂志1998,12(2)：74-76
137彭守静,王福楠.菟丝子、仙茅、巴戟天对人精子体外运动和膜功能影响的研究[J].中国中西医结合杂志,1997,17(3)：145-147
138曾金雄,戴西湖,刘建华,等.中药还精方对人类精子顶体反应的影响[J].中国中医药信息杂志,2002,9(4)：25-26
139曾金雄,戴西湖,刘建华,等.中药还精方对人精子运动及相关参数的影响[J].中国男科学杂志,2002,16(4)：319-320
140曾金雄,戴西湖,刘建华,等.首乌还精胶囊体外对人精子运动能力和受精能力的影响[J].中华男科学,2003,9(6)：476-479
141曾金雄,戴西湖,刘建华,等.首乌还精胶囊含药血清对人类精子运动能力和受精能力的影响[J].现代中西医结合杂志,2004,13(1)：21-22
142杨桂云,王佩娟,贾晓斌,等.补肾活血汤对小鼠体外受精及其早期胚胎发育的影响[J].中国中西医结合杂志,2001,21(7)：522-524
143陈怡,孙慧兰.固肾安胎中药对小鼠早期胚胎体外发育的影响[J].中国中药杂志,2008,33(14)：1767-1768
144 Racowsky C. High rates of embryonic loss, yet high incidence of multiple births in human ART:is this paradoxical?[J]. Theriogenology,2002,57(1):87-96
145张树成,陈秋梅,夏誉薇,等.补肾助孕方药促进人子宫内膜血管生成的实验研究[J].中 医药学刊,2004,22(5)：820-822
146黄冬梅,黄光英,陆付耳.补肾益气和血方中药对胚泡着床障碍小鼠子宫内膜表面胞饮突表达的影响[J].中华妇产科杂志,2004,39(4)：：230-233
147吴云霞,黄光英.补肾益气活血汤对小鼠血清雌、孕激素浓度及其受体表达的影响[J].现代生物医学进展,2006,6(2)：19-21
148吴云霞,黄光英.补肾益气活血方对吲哚美辛致胚泡着床障碍模型小鼠胚泡着床的影响[J].中国医学研究与临床,2005,4(3)：1-4
149吴云霞,黄光英,龚萍,等.吲哚美辛致小鼠胚泡着床障碍模型的建立[J].中国药理学通报,2005,21(3)：373-375
150王素霞,孙玉英.安胎合剂对GnRHa超排卵小鼠子宫内膜形态学的影响[J].中医药学报,2006,34(5)：45-47
151张明敏,黄玉琴,曾克勤.补肾安胎方调节胚泡着床障碍小鼠雌、孕激素的实验研究[J].中国中西医结合杂志,2006,26(7)：625-628
152张明敏,黄玉琴.补肾安胎方改善胚泡着床障碍小鼠局部血管形成及其机制[J].华中科技大学学报(医学版),2006,35(6)：781-783
153张明敏,黄玉琴,程亮亮,等.补肾安胎方对胚泡着床障碍小鼠子宫内膜HB-EGF及其受体EGFR表达的影响[J].华中科技大学学报(医学版),2008,37(1)：85-88
154张明敏,程亮亮,董莉萍.补肾安胎方对胚泡着床障碍模型小鼠着床局部PGI2及其核受体的影响[J].中国中西医结合杂志,2008,28(3)：229-233
155张明敏,董莉萍,程亮亮.补肾安胎方对胚泡着床障碍小鼠胚胎着床局部COX-2蛋白和究mRNA表达的影响[J].山东中医药大学学报,2008,32(1)：58-61
156张明敏,尹红章.补肾安胎方对胚泡着床障碍小鼠子宫LIF及其受体表达的影响[J].中国妇幼保健,2008,23(24)：3436-3439
157 Chang R, Chung PH, Rosenwaks Z. Role of acupuncture in the treatment of female infertility [J]. Fertil Steril,2002,78(6):1149-1153
158管枫,马淑兰,陈伯英.电针对去卵巢大鼠下丘脑神经内分泌信号传导的影响[J].中西医结合学报,2009,7(6)：563-568
159 Bai YH, Lim SC, Song CH, et al. Electro-acupuncture reverses nerve growth factor abundance in experimental polycystic ovaries in the rat[J]. Gynecol Obstet Invest,2004,57(2):80-85
160 Manni L, Lundeberg T, Holmang A, et al. Effect of electro-acupuncture on ovarian expression of a 1 and β2 adrenoceptors, and P75 neurotrophin receptors in rats with steroid-induced polycystic ovaries[J]. Reprod Biol Endocrinol,2005,3:21
161 Stener-Victorin E, Rie K, Orie W, et al. Effect of electro-acupuncture stimulation of different frequencies and intensities on ovarian blood flow in anaesthetized rats with steroid-induced polycystic ovaries[J]. Reproductive Biology and Endocrinology,2004,2:16
162刘新玉,黄光英,张明敏.针刺对胚泡着床障碍大鼠胚泡着床及发育的影响[J].中国针灸,2007,27(6)：439-442
163 Stener-Victorin E, Wikland M, Waldenstrom U, et al. Alternative treatments in reproductive medicine:much ado about nothing:acupuncture-a method of treatment in reproductive medicine:lack of evidence of an effect does not equal evidence of the lack of an effect[J]. Hum Reprod,2002,17(8):1942-1946
2张树成,沈明,蔡连香,等.补肾调经和养血补肾方药调经、促排卵作用的实验研究[J].中医杂志,1999,40(6)：369-371
3连方,滕依丽,张建伟,等.二至天癸颗粒对体外受精-胚胎移植周期人卵泡液白血病抑制因子和卵细胞质量的影响[J].中国中西医结合杂志,2007,27(11)：976-979
4连方,孙振高,张建伟,等.二至天癸方对小鼠卵细胞质量影响的实验研究[J].中国中西医结合杂志,2004,24(7)：625-6275
5杨桂云,王佩娟,贾晓斌,等.补肾活血汤对小鼠体外受精及其早期胚胎发育的影响[J].中国中西医结合杂志,2001,21(7)：522-524
6丁原全,万绍晖,南春红,等.补肾复方对围绝经期大鼠卵巢VEGF影响的实验研究[J].中医药学刊,2006,24(4)：676-677
7顿文亮,方泰惠,张丽娟,等.补肾助孕方对LPD大鼠卵巢中VEGF蛋白表达的影响[J].中国实用医药,2008,3(25)：54-55
8刘艳巧,党慧敏,刘润侠,等.助孕胶囊对多囊卵巢综合征大鼠卵巢局部细胞因子表达的影响[J].中医杂志,2008,49(5)：453-455,458
9黄琼霞,周安方,曹继刚,等.补肾毓麟汤对肾虚性生殖功能障碍大鼠血清EGF及生殖器官EGFR表达影响的实验研究[J].湖北中医杂志,2007,29(9)：3-4
10连方,孙振高,穆琳,等.二至天癸颗粒提高卵细胞质量与小鼠卵巢内IGF-1R mRNA表达量关系的研究[J].中国中西医结合杂志,2006,26(5)：431-434
11陈怡,孙慧兰.固肾安胎中药对小鼠早期胚胎体外发育的影响[J].中国中药杂 志,2008,33(14)：1767-1768
12张树成,陈秋梅,夏誉薇,等.补肾助孕方药促进人子宫内膜血管生成的实验研究[J].中医药学刊,2004,22(5)：820-822
13吴云霞,黄光英.补肾益气活血方对吲哚美辛致胚泡着床障碍模型小鼠胚泡着床的影响[J].中国医学研究与临床,2005,4(3)：1-4
14王素霞,孙玉英.安胎合剂对GnRHa超排卵小鼠子宫内膜形态学的影响[J].中医药学报,2006,34(5)：45-47
15张明敏,黄玉琴.补肾安胎方改善胚泡着床障碍小鼠局部血管形成及其机制[J].华中科技大学学报(医学版),2006,35(6)：781-783
16周珍辉主编.动物细胞培养技术[M].中国环境科学出版社,2006：250
17 Mao J, Wu G, Smith MF, et al. Effects of culture medium, serum type, and various concentrations of follicle-stimulating hormone on porcine preantral follicular development and antrum formation in vitro[J]. Biol Reprod.2002,67(4):1197-203
18 Schoevers EJ, Kidson A, Verheijden JH, et al. Effect of follicle-stimulating hormone on nuclear and cytoplasmic maturation of sow oocytes in vitro[J]. Theriogenology.2003,59(9):2017-2028
19 Atef A, Francois P, Christian V, et al. The potential role of gap junction communication between cumulus cells and bovine oocytes during in vitro maturation[J]. Mol Reprod Dev.2005,71(3):358-367
20 Coticchio G, Rossi G, Borini A, et al. Mouse oocyte meiotic resumption and polar body extrusion in vitro are differentially influenced by FSH, epidermal growth factor and meiosis-activating sterol[J]. Hum Reprod, 2004,19(12):2913-2918
21 Yang SH, Son WY, Yoon SH, et al. Correlation between in vitro maturation and expression of LH receptor in cumulus cells of the oocytes collected from PCOS patients in HCG-primed IVM cycles[J]. Hum Reprod, 2005,20(8):2097-2103
22 Choi YH, Carnevale EM, Seidel GE, et al. Effects of gonadotropins on bovine oocytes mature in TCM-199[J]. Theriogenology,2001,56(4):661-670
23 Junk SM, Dharmarajan A, Yovich JL. FSH priming improves oocyte maturation, but priming with FSH or hCG has no effect on subsequent embryonic development in an in vitro maturation program[J]. Theriogenology,2003,59(8):1741-1749
24 Javed A, Jamil A, Rezaei-Zarchi S, et al. An in vitro comparative study of follicle stimulating hormone (FSH) and activin A effects on the maturation of preantral follicle-enclosed oocytes from immature Syrian mice[J]. Iran Biomed J,2008,12(2):85-92
25石少权,苏兆娟,丘彦.卵泡刺激素和卵丘细胞对小鼠卵母细胞体外成熟的影响[J].实用诊断与治疗杂志,2005,19(8)：549-551
26陈勇,夏国良,苏友强,等.体外培养条件下促性腺激素对昆明白小鼠卵母细胞成熟及卵丘扩展的影响[J].动物学报,2001,47(2)：203-208
27 Guler A, Poulin N, Mermillod P, et al. Effect of growth factors, EGF and IGF—I, and estradiol on in vitro maturation of sheep ooeytes[J]. Theriogenology,2000,54 (2):209-218
28 Kim MK, FibriantoYH, Oh H J, et al. Effects of estradiol-17beta and porgesterone supplementation on in vitro nuclear maturation of canine oocytes[J]. Theriogenology,2005,63 (5):1342-1353
29 Ali A, Sirard MA. The effect of 17-beta estradiol and protein supplement on the response to purified and recombinant follicle stimulating hormone in bovine oocyte[J]. Zygote,2002,10 (1):65-71
30 Zheng P, Si W, Bavister B, et al.17β-estradiol and progesterone improve in-vitro cytoplasmic maturation of oocytes from unstimulated prepubertal and adult rhesus monkeys[J]. Hum Reprod,2003,18(10):2137-2144
31陈媛,杨菁,李洁,等.促性腺激素及雌二醇对小鼠卵母细胞体外成熟的影响[J].武汉大学学报(医学版),2007,28(5)：568-572,587
32 Beker AR, Colenbrander B, Bevers MM. Effect of 17beta-estradiol on the in vitro maturation of bovine oocyte [J]. Theriognology,2002,58:1663-1673
33 Dode MA, Graves CN. Role of estradiol-17beta on nuclear and cytoplasmic maturation of pig oocytes[J]. Anim Reprod Sci,2003,78(1/2):99
34李江源主编.性腺疾病[M].天津科技翻译出版公司,1997：58
35 Gupta PS, Nandi S, Ravindranatha BM, et al. Effect of commercially available PMSG on maturation, fertilization and embryo development of buffalo oocytes in vitro[J]. Reprod Fertil Dev,2001,13(5-6):355-360
36王坤,林涛,赵明辉,等.孕马血清促性腺激素对狗卵母细胞体外核成熟的影响[J].安徽农业科学,2008,36(35)：15503-15504,15509
37 Boiso I, Marti M, Santalo J, et al.A confocal microscopy analysis of the spindle and chromosome configurations of human oocytes cryopreserved at the germinal vesicle and metaphase Ⅱ stage[J]. Hum Reprod.2002 Jul;17(7):1885-1891
38王晓梅,黄天华,谢庆东,等Dynein抑制剂对卵母细胞体外成熟及Cyclin B1 mRNA水平的影响[J].癌变·畸变·突变,2004,16(5)：267-271
39王晓梅,鞠桂芝,傅海青,等.4Gy X射线照射对EL-4细胞Cyclin B1及cdc2 mRNA水平的影响[J].辐射研究与辐射工艺学报,2004,22(1)：43-46
40 杨菁. 体外受精与胚胎移植技术临床进展http://dryang.jing. haodf. com/wenzhang/103348. htm,2009.12.1
41胡建华,姚明,崔淑芳.实验动物学教程[M].上海科学技术出版社,2009：568
42陈大元.受精生物学受精机制与生殖工程[M].科学出版社,2000：146
43许美解,李刚主编.动物繁殖技术[M].化学工业出版社,2009：50
44 Peter R. Brinsden原著.体外受精与辅助生殖Bourn Hall诊所临床实践与实验室操作指南第3版[M].人民卫生出版社,2009：342
45王心如,周作民.生殖医学[M].人民卫生出版社,2004：38-39
46杨利国.动物繁殖学[M].中国农业出版社,2003：157-158
47 Coleman T R, Dunphy WG. Cdc2 regulatury factors[J]. Curr Opin Cell Biol,1994,6 (6):877-882
48 Yoshida N, Mita K, Yamashita M. Comparative study of the moleculer mechanism of oocyte maturation in amphibians[J]. Comp Bioehem Physiol B Biochem Mol Biol,2000,126(2):189-197
49 Shimada M, Zeng W X, Terada T. Inhibition of phosphatidyli-nositol 3-kinase of mitogen-activated protein kinase leads to suppression of p34(cdc2)kinase activity and meiotic progression beyond the meiosis I stage in porcine oocytes surrounded with cumulus cells[J]. Biol Reprod,2001,65(2):442-448
50夏蕾,张岭.卵母细胞成熟过程MPF的调控和MPF的作用底物[J].疾病控制杂志,2007,11(6)：614-617
51詹启敏.分子肿瘤学[M].人民卫生出版社,2005：95
52桑润滋.动物繁殖生物技术[M].中国农业出版社,2002：25
53 Sagata N. Meiotic metaphase arrest in animal oocytes:its mechanisms and biological significance[J]. Trends Cell Biol,1996,6(1):22-28
54 Sun QY, Lai L, Park KW, et al. Dynamic events are differently mediated by microfilaments, microtubules, and mitogen-activated protein kinase during porcine oocyte maturation and fertilization in vitro[J]. Biol Reprod,2001,64(3):879-889
55 Wang Q, Sun QY. Evaluation of oocyte quality:Morphological, cellular and molecular predictors[J]. Reprod Fertil Dev,2007,19(1):1-12
56 Wang WH, Keefe DL. Prediction of chromosome misalignment among in vitro mature human oocytes by spindle imaging with the PolScope [J]. Fertil Steril,2002,78(5):1077-1081
57 Coticchio G, Sereni E, Serrao L, et al. What criteria for the definition of oocyte quality?[J]. Ann N Y Acad Sci,2004,1034:132-144
58 Wang WH, Meng L, Hackett RJ, et al. Limited recovery of meiotic spindles in living human oocytes after cooling-rewarming observed using polarized light microscopy [J]. Hum Reprod,2001,16(11):2374-2378
59崔龙波,周雪莹,崔志峰,等.不同因素对小鼠MⅡ期卵母细胞纺锤体的影响[J].安徽农业科学,2008,36(33)：14555-14557
60崔奎青,石德帧,杨素芳.兔卵母细胞去核方法的研究[J].中国畜牧兽医,2005,32(7)：32-34
61 Combelles CM, Cekleniak NA, Racowsky C, et al. Assessment of nuclear and cytoplasmic maturation in in-vitro mature human oocytes [J]. Hum Reprod,2002,17(4):1006-1016
62朱亮,陈士岭,邢福祺,等.培养环境的温度变化与人MⅡ期卵母细胞纺锤体形态学变化的关系[J].第四军医大学学报,2005,26(15)：1414—1416
63 Aman RR, Parks JE. Effects of cooling and rewarming on the meiotic spindle and chromosomes of in vitro-mature bovine oocytes[J]. Biol Reprod,1994,50(1):103-110
64张文红,孙筱放,陈欣洁,等.不同温度对小鼠卵母细胞纺锤体的影响[J].基础医学与临床,2005,25(8)：721-724
65杨美娟.组织标本固定方法与免疫组织化学染色效果的关系[J].解剖科学进展,2003,9(1)：89-90
66 Li Y, Feng HL, Cao YJ, et al. Confocal microscopic analysis of the spindle and chromosome configurations of human oocytes mature in vitro [J]. Fertil Steril,2006,85(4):827-832
67 Moon JH, Jee BC, Ku SY, et al. Spindle positions and their distributions in in vivo and in vitro mature mouse oocytes[J]. Hum Reprod,2005,20(8):2207-2210
68段恒.资冲颗粒灌胃和直肠给药促卵泡发育的实验研究[D].成都中医药大学,2003
69冯雪,周惠芳.卵泡发育不良综合征的研究进展[J].长春中医药大学学报,2009,25(2)：299-300
70连方,王玉东,张宁.中医药补肾促卵泡发育的研究进展[J].中国中西医结合杂志,2000,20(4)：313-314,317
71马景兰,马建新,王旭初,等.促孕丸在“试管婴儿”中的应用[J].中国中西医结合杂志,2005,25(6)：547-548
72黄舒娥.中药人工周期疗法配合辅助生育技术对体外受精胚胎种植率的影响[J].广州中医药大学学报,2006,23(4)：302-204
73谈勇,石川睦男.补肾调周法在体外授精-胚胎移植前应用的临床观察[J].中国中医药信息杂志,2001,8(12)：56-57
74连方,滕依丽,张建伟,等.二至天癸颗粒联合体外受精-胚胎移植治疗不孕症61例临床研究[J].中医杂志,2006,47(6)：439-441
75李东.补肾养血活血法联合体外受精-胚胎移植对妊娠率的影响[J].中医杂志,2008,49(12)：1084-1086
76许小凤,谈勇,陈秀玲,等.补肾活血中药对卵巢储备功能的影响[J].江苏中医药,2007,39(2)：18-21
77李东,郭佳.补肾调周法改善卵巢储备功能在辅助生殖技术中运用的临床研究[J].北京中医药大学学报,2008,31(2)：131-134
78徐珉,司徒仪,李丽芸,等.中医补肾法对高龄不孕妇女的卵巢储备功能的调控[J].广州中医药大学学报,2004,21(4)：267-271
79单志群,曾勇,胡晓东,等.补肾调冲法在试管婴儿助孕技术中的运用——附96例临床报告[J].中医药学报,2002,30(6)：10-11
80王宁生,雷燕,刘平,等.关于血清药理学的若干思考[J].中国中西医结合杂志,1999,19(5)：263
81李军昌,夏天,黄熙,等.中药复方药理的研究工具——血清药理学[J].中药药理与临床,1998,14(6)：44
82詹红生.含药血清实验方法及其在中药新药研制中的应用进展[J].浙江中医学院学报,2000,24(2)：70-81
83 Iwama H, Amagaya S, Ogihara Y, et al. Effect of shosaikoto,a Japanese and Chinese traditional herbal medical mixture, on the mitogentic activity of lipopolysaccharide:a new pharmacological testing method [J]. J Ethoharmacol,1987,21(1):45-53
84吴健宇,穆静,李仪奎.血清药理实验中异种动物血清对细胞的毒性作用和灭活后的减毒作用[J].中国药理学通报,2000,16(1)：118-119
1张丽珠.临床生殖内分泌与不育症第2版[M].北京：科学出版社,2006：491-576
2 Linda MacDonald Glenn,An Economi st Looks at the Fertility Industry,Oct 01 2008, available at http://stanford. well sphere, com/bioethics-article/an-economist-looks-at-the-fert ility-industry/403947
3王维华,孙青原主编.生育革命迎接试管婴儿新时代[M].北京：科学出版社,2007：188
4张树成,沈明,蔡连香,等.补肾调经和养血补肾方药调经、促排卵作用的实验研究[J].中医杂志,1999,40(6)：369-371
5连方,滕依丽,张建伟,等.二至天癸颗粒对体外受精-胚胎移植周期人卵泡液白血病抑制因子和卵细胞质量的影响[J].中国中西医结合杂志,2007,27(11)：976-979
6连方,孙振高,张建伟,等.二至天癸方对小鼠卵细胞质量影响的实验研究[J].中国中西医结合杂志,2004,24(7)：625-627
7王素霞,孙玉英.安胎合剂对GnRHa超排卵小鼠子宫内膜形态学的影响[J].中医药学报,2006,34(5)：45-47
8黄冬梅,黄光英,陆付耳.补肾益气和血方中药对胚泡着床障碍小鼠子宫内膜表面胞饮突表达的影响[J].中华妇产科杂志,2004,39(4)：230-233
9杨桂云,王佩娟,贾晓斌,等.补肾活血汤对小鼠体外受精及其早期胚胎发育的影响[J].中国中西医结合杂志,2001,21(7)：522-524
10 Sharon LaMothe, Birth of Octoplets Raises Questions About Fertility Industry, May 04 2009, available at http://stanford, wellsphere. com/pregnancy-fertility-article/birth-of-octoplets-r aises-questions-about-fertility-industry/662003
11 Foulot H, Ranoux C, Dubuisson JB, et al. In vitro fertilization without ovarian stimulation:a simplified protocol applied in 80 cycles [J]. Fertil Steril,1989,52 (4):617-621
12 Janssens RMJ, Lambalk CB, Vermeiden JPW, et al. Invitro fertilization in a spontaneous cycle:easy, cheap and realistic[J]. Hum Reprod,2000,15(2):314-318
13 D'Amato G, Caroppo E, Pasquadibisceglie A, et al. A novel protocol of ovulation induction with delayed gonadotropin-releasing hormone antagonist administration combined with high-dose recombinant follicle-stimulating hormone and clomiphene citrate for poor responders and women over 35[J]. Fertil Steril,2004,81(6):1572-1577
14 Williams SC, Gibbons WE, Muasher SJ, et al. Minimal ovarian hyperstimulation for in vitro fertilization using sequential clomiphene citrate and gonadotropin with or without the addition of a gonadotropin-releasing hormone antagonist [J]. Fertil Steril,2002,78(5):1068-1072
15 Weigert M, Krischker U, Pohl M, et al. Comparison of stimulation with clomiphene citrate in combination with recombinant follicle stimulation with a gonadotropin-releasing hormone agonist protocol:a prospective, randomized study [J]. Fertil Steril,2002,78(1):34-39
16 Engel JB, Ludwig M, Felberbaum R, et al. Use of cetrorelix in combination with clomiphene citrate and gonadotropins:a suitable approach to" friendly IVF" [J]. Hum Reprod,2002,17(8):2 022-2026
17 Hwang JL, Huang LW, Hsieh BC, et al. Ovarian stimulation by clomiphene citrate and hMG in combination with centrorelix acetate for ICSI cycles[J]. Hum Reprod,2003,18(1):45-49
18 Palermo G, Joris H, Devroey P, et al. Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte[J].1992,340(8810):17-18
19李蓉,庄广伦,张敏芳.卵母细胞单精子显微注射治疗男性因素及不明原因小育[J].中华妇产科杂志,1997,32(4)：211-213
20 Handyside AH, Scriven PN, Ogilvie CM. The future of preimplantation genetic diagnosis[J]. Hum Reprod,1998,13 Suppl 4:249-255
21广东中山大学附属第一医院庄广伦教授日前指出——我国PGD技术应用要树立中国特色http://www. cnpharm. com/paperpdf/2005/Yy5-0820. pdf,2005.8.20
22 乔杰,张丽珠.中国大陆辅助生殖技术现状、挑战和发展http://byby.39.net/103/1/1167267, html,2010.3.1
23 Cha KY, Koo JJ, Ko JJ, et al. Pregnancy after in vitro fertilization of human follicular oocytes collected from nonstimulated cycles, their culture in vitro and their transfer in a donor oocyte program[J]. Fertil Steril,1991,55(1):109-113
24 乔 杰 . 试 管 婴 儿 新 技 术 须 谨 慎 推广http://www. sciencenet. cn/htmlnews/20079189538935189865.html,2007.9.18
25 Geoffrey Planer, World's first'ice baby'-25th birthday,30 March 2009, available at http://www. ivf. net/ivf/world_s_first_ice_baby_25th_birthday-o4078. html
26中国科学技术协会.中国科学技术专家传略医学编临床医学卷3[M].人民卫生出版社,2007：66
27 中国首名冷冻卵子技术培育的试管婴儿已问世 http://www. chinanews. com. cn/n/2004-05-11/26/434782. shtml,2004.5.11
28 国 内 首 例 “ 三 冻 ” 试 管 婴 儿 在 京 诞生http://news. xinhuanet. com/health/2006-02/10/content_4162145. htm,2006.2.10
29 Thomson JA, Itskovitz-Eldor J, Shapiro SS, et al. Embryonic stem cell lines derived from human blastocysts[J]. Science,1998,282(5391):1145-1147
30 Shamblott MJ, Axelman J, Wang S, et al. Derivation of pluripotent stem cells from cultured human primordial germ cell [J]. Proc Natl Acad Sci USA,1998,95(23):13726-13731
31 Chen Y, He ZX, Liu A, et al. Embryonic stem cells generated by nuclear transfer of human somatic nuclei into rabbit oocytes [J]. Cell Res,2003,13(4)-.251-263
32 Hubner K, Fuhrmann G, Christenson LK, et al. Derivation of oocytes from mouse embryonic stem cells [J]. Science,2003,300(5623):1251-1256
33 Vogel G. Embryonic stem cells. Scientists make sperm in a dish[J]. Science,2003,302 (5652):1875
34 Zhang J, Wang CW, Krey L, et al. In vitro maturation of human preovulatory oocytes reconstructed by germinal vesicle transfer[J]. Fertil Steril,1999,71(4):726-731
35 中 国 第 四 代试管婴 儿武汉 出 生 将 是 唯 一 一例http://www. chinanews. com. cn/n/2004-02-25/26/406288. html,2004.2.25
36乔杰.人类辅助生殖技术的新进展[J].中国实用妇科与产科杂志,2008,24(1)：33-34
37王克华,李娟,张琦等.卵子赠送技术的应用及进展[J].国外医学计划生育分朋,2003,22(2)：94-96
38张松英主编.妇产科疾病临床治疗与合理用药[M].科学技术文献出版社,2007：344
39 Movahedin M, Ajeen A, Ghorbanzadeh N, et al. In vitro maturation of fresh and frozen-thawed mouse round spermatids[J]. Andrologia,2004,36(5):269-276
40 Badawy A, Elnashar A, Totongy M. Clomiphene citrate or aromatase inhibitors for superovulation in women with unexplained infertility undergoing intrauterine insemination:a prospective randomized trial[J]. Fertil Steril,2009,92(4):1355-1359
41 Patchava S, Gelbaya TA, Nardo LG. Managing the complications associated with assisted reproductive technologies[J]. Minerva Ginecol,2009,61 (4):347-355
42王芳,孙莹璞,孔慧娟,等.不同辅助生殖技术妊娠分娩的1540例新生儿出生缺陷分析[J].中华妇产科杂志,2009,44(2)：108-111
43 Multiple gestation pregnancy. The ESHRE Capri Workshop Group[J]. Hum Reprod,2000,15(8):1856-1864
44 Schieve LA. Multiple-gestation pregnancies after assisted reproductive technology treatment:population trends and future directions[J]. Women's Health, 2007,3 (3):301-307
45 Schnorr JA, Doviak MJ, Muasher SJ, et al. Impact of a cryop reservation p rogram on the multiple pregnancy rates associated with assisted reproductive technologies[J]. Fertil Steril,2001,75(1):147-151
46钟刚,蔡桂茹.助孕技术对多胎妊娠发生率的影响[J].国外医学.妇产科学分册,1999,26(2)：112
47 Shulman A, Ghetler Y, Beyth Y, et al. The significance of an early (premature) rise of plasma progesterone in in vitro fertilization cycles induced by a "long protocol" of gonadotropin releasing hormone analogue and human menopausal gonadotropins[J]. J Assist Reprod Genet,1996,13(3):207-211
48 Ertzeid G, Storeng R. The impact of ovarian stimulation on implantation and fetal development in mice[J]. Hum Reprod,2001,16(2):221-225
49 Shi W, Haaf T. Aberrant methylation patterns at the two-cell stage as an indicator of early development failure[J]. Mol Reprod Dev,2002,63(3):329-334
50 Chang AS, Moley KH, Wangler M, et al. Association between Beckwith-Wiedemann syndrome and assisted reproductive technology:a case series of 19 patients[J]. Fertil Steril,2005,83(2):349-354
51 Shevell T, Malone FD, Vidaver J, et al. Assisted reproductive technology and pregnancy outcome[J]. Obstet Gynecol,2005,106(5 Pt 1):1039-1045
52 Bohlmann MK, Fritzsching B, Luedders DW, et al. Impact of assisted reproduction on obstetrics and neonatology[J]. Z Geburtshilfe Neonatol,2009,213(6):221-227
53 Allen C, Bowdin S, Harrison RF, et al. Pregnancy and perinatal outcomes after assisted reproduction:a comparative study[J]. Ir J Med Sci,2008,177(3):233-241
54 Wang JX, Norman RJ, Wilcox AJ. Incidence of spontaneous abortion among pregnancies produced by assisted reproductive technology[J]. Hum Reprod,2004,19(2):272-277
55 Wang YA, Sullivan EA, Black D, et al. Preterm birth and low birth weight after assisted reproductive technology-related pregnancy in Australia between 1996 and 2000[J]. Fertil Steril.2005,83(6):1650-1658
56 Jackson RA, Gibson KA, Wu YW, et al. Perinatal outcomes in singletons following in vitro fertilization:a meta-analysis[J], Obstet Gynecol,2004,103(3):551-563
57 Koivurova S, Hartikainen AL, Gissler M, et al. Neonatal outcome and congenital malformations in children born after in-vitro fertilization[J]. Hum Reprod, 2002,17(5):1391-1398
58 Schieve LA, Meikle SF, Ferre C, et al. Low and very low birth weight in infants conceived with use of assisted reproductive technology[J]. N Engl J Med,2002,346(10):731-737
59 Strandell A, Thorburn J, HambergerL. Risk factors for ectop ic p regnancy in assisted rep roduction[J]. Fertil Steril JT Fertility and Sterility,1999,71 (2):282-286
60 Alsunaidi M. Incidence of ectopic pregnancy after assisted reproduction treatment[J].2007,28 (4):590-592
61 Rossing MA, Tang MT, Flagg EW, et al. A case control study of ovarian cancer in relation to infertility and the use of ovulation inducing drugs [J]. Am J Epidemiol,2004,160(11):1070-1078
62 Halliday JL, Ukoumunne OC, Baker HW, et al. Increased risk of blastogenesis birth defects, arising in the first 4 weeks of pregnancy, after assisted reproductive technologies[J]. Hum Reprod,2010,25(1):59-65
63 Reefhuis J, Honein MA, Schieve LA, et al. Assisted reproductive technology and major structural birth defects in the United States[J]. Hum Reprod,2009,24 (2):360-366
64 Kallen B, Finnstrom 0, Nygren KG, et al. In vitro fertilization(IVF) in Sweden:risk for congenital malformations after different IVF methods[J]. Birth Defects Res A Clin Mol Teratol,2005,73(2):162-169
65 Hansen M, Kurinczuk JJ, Bower C, et al. The risk of major birth defects after intracytoplasmic sperm injection and in vitro fertilization[J]. N Engl J Med,2002,346:725-730
66 Kuwata T, Matsubara S, Ohkuchi A, et al. The risk of birth defects in dichorionic twins conceived by assisted rep roductive technology [J]. Twin Res,2004,7 (3):223-227
67连方,明蔚.体外授精2胚胎移植患者降调节后肾虚本质探讨[J].山东中医药大学学报,2008,32(2)：109-110
68 Coyle M, Smith C. A survey comparing TCM diagnosis, health status and medical diagnosis in women undergoing assisted reproduction[J]. Acupunct Med, 2005,23 (2):62-69
69李东.温肾安胎饮联合辅助生殖技术提高胚胎移植失败患者妊娠率的临床研究[J].北京中医药大学学报,2009,32(2)：139-141
70张建伟.体外受精-胚胎移植降调节后中医辨证思路与方法[J].中医药通报,2007,6(3)：36-37
71连方,滕依丽,张建伟,等.二至天癸颗粒联合体外受精-胚胎移植治疗不孕症61例临床研究[J].中医杂志,2006,47(6)：439-441
72连方,滕依丽,张建伟,等.二至天癸颗粒对体外受精-胚胎移植周期人卵泡液白血病抑制因子和卵细胞质量的影响[J].中国中西医结合杂志,2007,27(11)：976-979
73许小凤,谈勇,陈秀玲,等.补肾活血中药对卵巢储备功能的影响[J].江苏中医药,2007,39(2)：18-21
74李东.补肾养血活血法联合体外受精-胚胎移植对妊娠率的影响[J].中医杂志,2008,49(12)：1084-1086
75许娟,冯玉华,汤丽莎,等.补肾活血方对促排卵中子宫内膜发育不良的影响[J].江西中医药,2007,38(5)：42-43
76单志群,曾勇,胡晓东,等.补肾调冲法在试管婴儿助孕技术中的运用——附96例临床报告[J].中医药学报,2002,30(6)：1011
77吴育宁.中医药辅助试管婴儿的治疗经验[J].中国中西医结合杂志,2007,27(3)：270-271
78李东,郭佳.补肾调周法改善卵巢储备功能在辅助生殖技术中运用的临床研究[J].北京中医药大学学报,2008,31(2)：131-134
79黄舒娥.中药人工周期疗法配合辅助生育技术对体外受精胚胎种植率的影响[J].广州中医药大学学报,2006,23(4)：302-204
80谈勇,石川睦男.补肾调周法在体外授精-胚胎移植前应用的临床观察[J].中国中医药信息杂志,2001,8(12)：56-57
81金保方,杨晓玉,张新东,等.中医治疗反复不明原因IVF失败后成功妊娠52例[J].南京中医药大学学报,2009,25(1)：56-58
82徐珉,司徒仪,李丽芸,等.中医补肾法对高龄不孕妇女的卵巢储备功能的调控[J].广州中医药大学学报,2004,21(4)：267-271
83连方,张建伟,张宁,等.中西医结合疗法在试管婴儿技术中的应用[J].山东中医药大学学报,2002,26(3)：182-183
84连方,张宁,张建伟,等.中药配合体外授精及胚胎移植治疗不孕症2例[J].中国中西医结合杂志,2002,22(8)：602
85姜朵生,丁玎.针药治疗持续不排卵性不孕症疗效观察[J].中国针灸,2009,29(1)：21-24
86姜萍,傅萍.傅萍运用养精种玉汤治疗试管婴儿术前后临床经验[J].浙江中医杂志,2009,44(3)：170-171
87张明敏,黄光英,陆付耳,等.补肾益气活血汤对多次助孕技术失败患者结局的影响[J].微循环学,2002,12(1)：10-13
88张明敏,黄光英,陆付耳,等.银杏叶制剂对体外授精治疗中卵巢反应低下患者的作用[J].中国中西医结合杂志,2003,23(3)：171-173
89马景兰,马建新,王旭初,等.促孕丸在“试管婴儿”中的应用[J].中国中西医结合杂志,2005,25(6)：547-548
90朱文杰,李雪梅,陈秀敏,等.滋肾育胎丸对体外受精-胚胎移植患者胚胎种植率的影响[J].中国中西医结合杂志,2002,22(10)：729-731
91徐国男.针灸加中药治疗对提高试管婴儿成功率的疗效观察[J].天津中医药,2006,23(4)：341-342
92陈海燕,夏国守.体外受精和胚胎移植技术中应用中医药对男性不育患者治疗结果影响的研究[J].现代中西医结合杂志,2005,14(9)：1240-1241
93金保方,黄宇烽,杨晓玉,等.养精胶囊治疗弱精子症的临床观察[J].南京中医药大学学报,2006,22(5)：286-289
94杨明,王树声,陈志强,等.中药对肾阳虚不育症患者精子DNA的影响[J].现代中西医结合杂志,2002,11(10)：889
95翟亚春.男子免疫性不育症辨治思路初探[J].江苏中医,2000,21(11)：15
96倪江,张威,边淑玲,等.消抗安胎液对男性血清抗精子抗体及白细胞介素2受体水平的影响[J].中医药学报,1999,4：49
97 Zhang M, Huang G, Lu F, et al. Influence of acupuncture on idiopathic male infertility in assisted reproductive technology [J]. J Huazhong Univ Sci Technolog Med Sci,2002,22(3):228-230
98 Stener-Victorin E, Humaidan P. Use of acupuncture in female infertility and a summary of recent acupuncture studies related to embryo transfer[J]. Acupunct Med,2006,24(4):157-163
99 Cai X. Substitution of acupuncture for human chorionic gonadotropin in ovulation induction[J]. J Tradit Chin Med,1997,17(2):119
100 Stener-Victorin E, Waldenst rom U, Tagnfors U, et al. Effect s of electro-acupuncture on anovulation in women with polycystic ovary syndrome[J]. Acta Obstet Gynecol Scand,2000,79:180
101 Magarelli PC, Cridennda DK, Cohen M. Changes in serum cortisol and prolactin associated with acupuncture during controlled ovarian hyperstimulation in women undergoing in vitro fertilization-embryo transfer treatment[J]. Fertil Steril,2009,92(6):1870-1879
102桑海莉.补肾加针刺治疗多囊卵巢综合征35例临床观察[J].中医杂志,2000,41(7)：412
103俞瑾.多囊卵巢综合征的中西医治疗[J].中国实用妇科与产科杂志,2002,18(11)：651
104杨越红,洪建云,魏达友,等.针刺治疗多囊卵巢综合征所致不孕的临床观察[J].广东医学院学报,2005,23(4)：377
105陈栋,陈恕仁,石晓兰,等.针挑治疗多囊卵巢综合征临床观察[J].中国针灸,2007,27(2)：9-11
106宋丰军,郑士立,马大正.针灸治疗排卵障碍性不孕症临床观察[J].中国针灸,2008,28(1)：21-23
107连方,李海仙,张建伟,等.电针促使LUFS病人排卵的临床研究[J].上海针灸杂志,2006,25(8)：3-4
108董纪翠.电针治疗卵泡发育不良临床观察[J].中国中医药信息杂志,2007,14(9)：68-69
109陈军,刘莉莉,崔薇,等.电针干预对卵巢低反应患者体外受精胚胎移植的影响[J].中国针灸,2009,29(10)：775-779
110 Stener-Victorin E, Waldenstrom U, Andersson SA, et al. Reduction of blood flow impedance in the uterine arteries of infertile women with elect ro-acupuncture[J]. Hum Reprod,1996,11:1314
111 Manheimer E, Zhang G, Udoff L, et al. Effects of acupuncture on rates of pregnancy and live birth among women undergoing in vitro fertilisation:systematic review and meta-analysis[J]. BMJ,2008,336(7643):545-549
112 Cheong YC, Hung Yu Ng E, Ledger WL. Acupuncture and assisted conception[J]. Cochrane Database Syst Rev,2008,8(4):CD006920
113 Paulus WE, Zhang MM, Strehler Erwin, et al. Influence of acupuncture on the pregnancy rate in patients who undergo assisted reproduction therapy[J]. Fertil Steril,2002,77:721-724
114张明敏,黄光英,陆付耳,等.针刺对胚胎移植怀孕率的影响及其机理：随机安慰对照研究 [J].中国针灸,2003,23(1)：3-5
115 Westergaard LG, Mao Q, Krogslund M, et al. Acupuncture on the day of embryo transfer significantly improves the reproductive outcome in infertile women:a prospective, randomized trial[J]. Fertil Steril,2006,85(5):1341-1346
116 Johnson D. Acupuncture prior to and at embryo transfer in an assisted conception unit—a case series [J]. Acupunct Med,2006,24(1):23-28
117 Smith C, Coyle M, Norman RJ. Influence of acupuncture stimulation on pregnancy rates for women undergoing embryo transfer [J]. Fertil Steril,2006,85(5):1352-1358
118 Dieterle S, Ying G, Hatzmann W, et al. Effect of acupuncture on the outcome of in vitro fertilization and intracytoplasmic sperm injection:a randomized, prospective, controlled clinical study[J]. Fertil Steril,2006,85(5):1347-1351
119崔薇,孙伟,刘莉莉,等.电针干预对体外受精-胚胎移植患者的作用研究[J].中国妇幼保健,2007,22(24)：3403-3405
120崔薇,刘莉莉,孙伟,等.电针对不同证型不孕症患者体外受精-胚胎移植作用的研究[J].中国针灸,2008,28(4)：254-256
121谢广妹.菟丝子水提取物治疗卵巢过度刺激综合征实验研究[J].兰州大学学报(医学版),2009,35(3)：4-26,31
122林丽波,姚美玉,吴效科,等.卵巢过度刺激综合征的中医治疗概述[J].世界中西医结合杂志,2009,4(3)：255256
123辛明蔚.连方预防卵巢过度刺激综合征的经验[J].山东中医药大学学报,2007,31(6)：476-477
124刘凤云,张敏,吴先哲.中医药治疗卵巢过度刺激综合征的思路与方法[J].中医杂志,2004,45(2)：143-144
125俞而慨,归绥琪,邹琴娣.中西医结合治疗中重度卵巢过度刺激综合征的临床观察[J].中国中西医结合杂志,2003,23(3)：175-177
126孙永忠,费小阳,沈云华,等.中西医结合治疗卵巢过度刺激综合征临床观察[J].实用中医药杂志,2007,23(10)：660-661
127刘颖,刘云鹏.中西医结合治疗卵巢过度刺激综合征体会[J].中国中医药信息杂志,2004,11(11)：1011-1012
128林敏.中西医结合治疗卵巢过度刺激综合征[J].四川中医,2003,21(4)：58-59
129孙伟,张敏.中西医结合治疗重度卵巢过度刺激综合征[J].现代中西医结合杂志,2009,18(26)：3211,3249
130张晓红,杨正望,尤昭玲.补肾中药对卵巢功能及子宫内膜的影响[J].中医药学刊,2005,23(11)：2054-2055
131 Huang ST, Chen AP. Traditional Chinese medicine and infertility[J]. Curr Opin Obstet Gynecol,2008,20(3):211-215
132 Huang BM, Hsiao KY, Chuang PC, et al. Upregulation of steroidogenic enzymes and ovarian 17beta-estradiol in human granulosa-lutein cells by Cordyceps sinensis mycelium[J]. Biol Reprod,2004,70(5):1358-1364
133连方,孙振高,张建伟,等.二至天癸方对小鼠卵细胞质量影响的实验研究[J].中国中西医结合杂志,2004,24(7)：625-627
134连方,孙振高,穆琳,等.二至天癸颗粒提高卵细胞质量与小鼠卵巢内IGF-1R mRNA表达量关系的研究[J].中国中西医结合杂志,2006,26(5)：431-434
135戴锦成,周小平.宝生汤对生精障碍小鼠睾丸及精子的影响[J].福建中医学院学报,2002,12(4)：34
136江峰,王益鑫,盛新福,等.体外添加黄芪对精子质量的影响[J].男性学杂志1998,12(2)：74-76
137彭守静,王福楠.菟丝子、仙茅、巴戟天对人精子体外运动和膜功能影响的研究[J].中国中西医结合杂志,1997,17(3)：145-147
138曾金雄,戴西湖,刘建华,等.中药还精方对人类精子顶体反应的影响[J].中国中医药信息杂志,2002,9(4)：25-26
139曾金雄,戴西湖,刘建华,等.中药还精方对人精子运动及相关参数的影响[J].中国男科学杂志,2002,16(4)：319-320
140曾金雄,戴西湖,刘建华,等.首乌还精胶囊体外对人精子运动能力和受精能力的影响[J].中华男科学,2003,9(6)：476-479
141曾金雄,戴西湖,刘建华,等.首乌还精胶囊含药血清对人类精子运动能力和受精能力的影响[J].现代中西医结合杂志,2004,13(1)：21-22
142杨桂云,王佩娟,贾晓斌,等.补肾活血汤对小鼠体外受精及其早期胚胎发育的影响[J].中国中西医结合杂志,2001,21(7)：522-524
143陈怡,孙慧兰.固肾安胎中药对小鼠早期胚胎体外发育的影响[J].中国中药杂志,2008,33(14)：1767-1768
144 Racowsky C. High rates of embryonic loss, yet high incidence of multiple births in human ART:is this paradoxical?[J]. Theriogenology,2002,57(1):87-96
145张树成,陈秋梅,夏誉薇,等.补肾助孕方药促进人子宫内膜血管生成的实验研究[J].中 医药学刊,2004,22(5)：820-822
146黄冬梅,黄光英,陆付耳.补肾益气和血方中药对胚泡着床障碍小鼠子宫内膜表面胞饮突表达的影响[J].中华妇产科杂志,2004,39(4)：：230-233
147吴云霞,黄光英.补肾益气活血汤对小鼠血清雌、孕激素浓度及其受体表达的影响[J].现代生物医学进展,2006,6(2)：19-21
148吴云霞,黄光英.补肾益气活血方对吲哚美辛致胚泡着床障碍模型小鼠胚泡着床的影响[J].中国医学研究与临床,2005,4(3)：1-4
149吴云霞,黄光英,龚萍,等.吲哚美辛致小鼠胚泡着床障碍模型的建立[J].中国药理学通报,2005,21(3)：373-375
150王素霞,孙玉英.安胎合剂对GnRHa超排卵小鼠子宫内膜形态学的影响[J].中医药学报,2006,34(5)：45-47
151张明敏,黄玉琴,曾克勤.补肾安胎方调节胚泡着床障碍小鼠雌、孕激素的实验研究[J].中国中西医结合杂志,2006,26(7)：625-628
152张明敏,黄玉琴.补肾安胎方改善胚泡着床障碍小鼠局部血管形成及其机制[J].华中科技大学学报(医学版),2006,35(6)：781-783
153张明敏,黄玉琴,程亮亮,等.补肾安胎方对胚泡着床障碍小鼠子宫内膜HB-EGF及其受体EGFR表达的影响[J].华中科技大学学报(医学版),2008,37(1)：85-88
154张明敏,程亮亮,董莉萍.补肾安胎方对胚泡着床障碍模型小鼠着床局部PGI2及其核受体的影响[J].中国中西医结合杂志,2008,28(3)：229-233
155张明敏,董莉萍,程亮亮.补肾安胎方对胚泡着床障碍小鼠胚胎着床局部COX-2蛋白和究mRNA表达的影响[J].山东中医药大学学报,2008,32(1)：58-61
156张明敏,尹红章.补肾安胎方对胚泡着床障碍小鼠子宫LIF及其受体表达的影响[J].中国妇幼保健,2008,23(24)：3436-3439
157 Chang R, Chung PH, Rosenwaks Z. Role of acupuncture in the treatment of female infertility [J]. Fertil Steril,2002,78(6):1149-1153
158管枫,马淑兰,陈伯英.电针对去卵巢大鼠下丘脑神经内分泌信号传导的影响[J].中西医结合学报,2009,7(6)：563-568
159 Bai YH, Lim SC, Song CH, et al. Electro-acupuncture reverses nerve growth factor abundance in experimental polycystic ovaries in the rat[J]. Gynecol Obstet Invest,2004,57(2):80-85
160 Manni L, Lundeberg T, Holmang A, et al. Effect of electro-acupuncture on ovarian expression of a 1 and β2 adrenoceptors, and P75 neurotrophin receptors in rats with steroid-induced polycystic ovaries[J]. Reprod Biol Endocrinol,2005,3:21
161 Stener-Victorin E, Rie K, Orie W, et al. Effect of electro-acupuncture stimulation of different frequencies and intensities on ovarian blood flow in anaesthetized rats with steroid-induced polycystic ovaries[J]. Reproductive Biology and Endocrinology,2004,2:16
162刘新玉,黄光英,张明敏.针刺对胚泡着床障碍大鼠胚泡着床及发育的影响[J].中国针灸,2007,27(6)：439-442
163 Stener-Victorin E, Wikland M, Waldenstrom U, et al. Alternative treatments in reproductive medicine:much ado about nothing:acupuncture-a method of treatment in reproductive medicine:lack of evidence of an effect does not equal evidence of the lack of an effect[J]. Hum Reprod,2002,17(8):1942-1946