草鱼肝脏型脂肪酸结合蛋白基因克隆及原核表达
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摘要
肝脏型脂肪酸结合蛋白(liver-type fatty acid-binding protein,L-FABP)属于脂肪酸结核蛋白家族一员,为一族小分子蛋白质,对长链脂肪酸有很高的亲和力,能把脂肪酸从细胞膜转运到氧化和合成的部位,在长链脂肪酸的代谢中发挥着非常重要的作用。本文运用RACE(rapid amplification of cDNA ends)-PCR方法扩增出草鱼肝脏型脂肪酸结合蛋白基因的全长cDNA,其全长为487 bp,包含5′端非编码区55 bp,3′端非编码区51 bp,开放阅读框381 bp。草鱼脂肪酸结合蛋白的开放阅读框编码126个氨基酸。半定量RT-PCR结果显示草鱼L-FABP mRNA主要在肝脏、脾脏、后肠中表达,在头肾、后肾、心脏、前肠表大量较少,在中肠、肌肉和腮中几乎不表达。
根据草鱼L-FABP的cDNA序列设计含有酶切位点的引物,将草鱼L-FABP完整开放阅读框克隆到原核融合表达载体pET-32a(+)上,测序鉴定后,将重组表达质粒转入大肠杆菌BL21(DE3)中用IPTG进行诱导表达,经SDS-PAGE电泳分析显示重组融合蛋白在分子量约33 kDa左右处有明显表达带,与预期分子量大小一致,光密度分析,目的蛋白约占总表达蛋白的49.5%。这一研究成果为进一步研究脂肪酸结合蛋白生物学特性以及脂肪酸结合蛋白基因对脂肪代谢调控规律奠定了基础。
Liver-type Fatty acid-binding protein(L-FABP) as a member of Fatty acid-binding protein family is a key protein involved in the metabolism of long-chain fatty acids.It has a high affinity with long-chain fatty acids and help long-chain fatty acids transfer to the cell membrane which has sites of fatty acids oxidation and synthesis.In the study,L-FABP cDNA was cloned from grass carp (Ctenopharyngodon idellus) applying RACE(rapid amplification of cDNA ends)-PCR.The full-length cDNA of Liver type fatty acid-binding protein(L-FABP) was cloned from liver of grass carp.It consisted of a sequence of 487 bp comprising a 55 bp 5' untranslated region(UTR),a 51 bp 3'-UTR and a 381 bp open reading frame(ORF) encoding an peptide,consisting of 126 amino acids.Grass carp L-FABP mRNA mainly expressed in the liver,spleen,ileum,less in foregut,head kidney and body kidney.In the mid-intestine,muscle and gill there was nearly no expression by Reverse transcriptase polymerase chain reaction.
The primers coding for the open reading frames of L-FABP were designed, which contain two enzymes sites,the same as vector pET32 contains.The ORF of L-FABP with was cloned into vector pET-32a(+) after digested by enzymes,then constructed a prokaryotic expression vector pET-32a-L-FABP with a T7 promoter. Recombinant plasmid identified by sequencing,and then transformed into E.coli BL21(DE3) for expression,then induced by IPTG.The products of expression was detected by SDS-PAGE.The result showed that a 33 KD fusion protein was obtained, including 49.5%of total protein.The result provided foundation for further to research to the biological function of fatty acid-binding protein on fatty acid metabolism.
根据草鱼L-FABP的cDNA序列设计含有酶切位点的引物,将草鱼L-FABP完整开放阅读框克隆到原核融合表达载体pET-32a(+)上,测序鉴定后,将重组表达质粒转入大肠杆菌BL21(DE3)中用IPTG进行诱导表达,经SDS-PAGE电泳分析显示重组融合蛋白在分子量约33 kDa左右处有明显表达带,与预期分子量大小一致,光密度分析,目的蛋白约占总表达蛋白的49.5%。这一研究成果为进一步研究脂肪酸结合蛋白生物学特性以及脂肪酸结合蛋白基因对脂肪代谢调控规律奠定了基础。
Liver-type Fatty acid-binding protein(L-FABP) as a member of Fatty acid-binding protein family is a key protein involved in the metabolism of long-chain fatty acids.It has a high affinity with long-chain fatty acids and help long-chain fatty acids transfer to the cell membrane which has sites of fatty acids oxidation and synthesis.In the study,L-FABP cDNA was cloned from grass carp (Ctenopharyngodon idellus) applying RACE(rapid amplification of cDNA ends)-PCR.The full-length cDNA of Liver type fatty acid-binding protein(L-FABP) was cloned from liver of grass carp.It consisted of a sequence of 487 bp comprising a 55 bp 5' untranslated region(UTR),a 51 bp 3'-UTR and a 381 bp open reading frame(ORF) encoding an peptide,consisting of 126 amino acids.Grass carp L-FABP mRNA mainly expressed in the liver,spleen,ileum,less in foregut,head kidney and body kidney.In the mid-intestine,muscle and gill there was nearly no expression by Reverse transcriptase polymerase chain reaction.
The primers coding for the open reading frames of L-FABP were designed, which contain two enzymes sites,the same as vector pET32 contains.The ORF of L-FABP with was cloned into vector pET-32a(+) after digested by enzymes,then constructed a prokaryotic expression vector pET-32a-L-FABP with a T7 promoter. Recombinant plasmid identified by sequencing,and then transformed into E.coli BL21(DE3) for expression,then induced by IPTG.The products of expression was detected by SDS-PAGE.The result showed that a 33 KD fusion protein was obtained, including 49.5%of total protein.The result provided foundation for further to research to the biological function of fatty acid-binding protein on fatty acid metabolism.
引文
[1]姜延志,李学伟,杨光希.猪L-FABP基因的克隆、表达特征及遗传多态性研究,遗传学报,2006,11(33):606-611.
[2]Eileen M Denovan Wright,Marianne Pierce,Mukesh K Sharma,et al.cDNA sequence and tissue-specific expression of a basic liver-type fatty acid binding protein in adult zebra fish (Danio rerio)[J].Biochemical & Biophysical Acta,2000,1492:227-232.
[3]庞卫军,白亮,杨公社.西部地区主要猪种H-FABP基因多态性,IMF含量及不同基因型脂肪细胞脂[J].遗传学报,2002,33(6):515-524.
[4]黄治国,熊俐,刘振山,等.绵羊肌肉H-FABP和PPARγ基因表达的发育性变化及其对肌内脂肪含量的影响[J].遗传学报,2002,33(6):507-514.
[5]Santiago M Di Pietro,Jose A Santome.Structural and Biochemical Characterization of the Lungfish(Lepidosiren paradoxa) Liver Basic Fatty Acid Binding Protein[J].Archives of Biochemistry and Biophysics,2001,388:81-90.
[6]庞卫军,杨公社.8个猪种和野猪H-FABP基因分子标记与IMF含量的关系[J].农业生物技术学报,2005,13(6):733-738.
[7]王启贵,李宁,邓学梅,等.鸡细胞外脂肪酸结合蛋白基因单核苷酸多态性与腹脂性状的相关研究[J].中国科学(C辑),2001,31(3):266-270.
[8]Gentili C.Cemetfi S.Tacchei C et al Expression of the extracellular fatty binding protein during muscle fiber formation,Exp Cell Res,1998,24:410-418.
[9]Cancedda F D,Malpet[M],Gentili C,et al The developmentally regulated avian Chil Jpocalin is an extracetlul as fatty acid-binding protein J Biol Chem.1996.377{J0):633-638
[10]Gerhen F Harders F VeeTkamp J Effect of genetic variant of bean fatty acid binding protein gene on intramuscular fat and performance traits in pig[J].Animal Science.1999,77:846-852
[11]王启贵,李宁,邓学梅,等.鸡脂肪酸结合蛋白基因的克隆和测序分析[J].遗传学报,2002,29(2):115-118.
[12]赵春江,李宁,邓学梅.AS-PCR技术检测鸡EX-FABP基因型方法的建立[J].农业生物技术学报,2003,11(3):273-275.
[13]庞卫军,杨公社,龙火生,等.猪心脏脂肪酸结合蛋白基因PCR-RFLP分子标记研究[K].生物技术通讯,2004,15(2):124-127.
[14]Eileen M.Denovan-Wright,Marianne Pierce,Mukesh K.Sharma,et al.cDNA sequence and tissue-specific expression of a basic liver-type fatty acid binding protein in adult zebra fish (Danio rerio)[J].Biochimica & Biophysical Acta,2000,1492:227-232
[15]Ockner RK,Manning JA,and Kane JP.Fatty acid binding protein.Isolation from rat liver, characterization, and immunochemical quantification [J]. J. Biol. Chem., 1982, 257: 7872— 7878.
[16]Santiago M. Di Pietro, Jose A. Santome. Structural and Biochemical Characterization of the Lungfish (Lepidosiren paradoxa) Liver Basic Fatty Acid Binding Proteinl [J]. Archives of Biochemistry and Biophysics, 2001, 388: 81—90
[17] Maatman RG, van de Westerlo EM, van Kuppevelt TH, et al. Molecular identification of the liver and the heart-type fatty acid-binding proteins in human and rat kidney. Use of the reverse transcriptase polymerase chain reaction [J]. Biochem J, 1992, 288 (1): 285-290.
[18] Guor Mour Her, Yang-Hui Yeh, Jen-Leih Wu. Functional Conserved Elements Media-te Intestinal-Type Fatty Acid Binding Protein (I-FABP) Expression in the Gut Epithel-ia of Zebra fish Larvae[J]. Developmental Dynamics, 2004, 230: 734—742
[19] Gerbens F,De Koning D J,Harders F L, et al.The effect of adiposity and heart fatty acid-binding protein genes on intramuscular fat and back fat content in Meishan crossbed pigs[J].Anima Sci,2000,78 (3): 552—529.
[20] Hertzel AV, Bennaars-Eiden A, Bemlohr DA.Increased lipolysis in transgenic animals over expressing the epithelial fatty acid binding protein in adipose cells [J]. Lipid Res, 2002, 43(12): 2105—2111
[21] Bass, N.M. & Manning, J.A. Tissue expression of three structurally different fatty acid binding proteins from rat heart muscle, liver, and intestine[J]. Biochem. Biophys. Res. Commun. 1986,137, 929—935.
[22] Paulussen, R.J.A., van Moerkerk, H.T.B. & Veerkamp, J.H. Immunochemical quantitation of fatty acid-binding proteins. Tissue distribution of liver and heart FABP types in human and porcine tissues[J]. Int. J. Biochem. 1990, 22, 393—398.
[23] Kurtz, A., Zimmer, A., Schnu tgen, F., Bruning, G., Spener, F . & Muller, T. The expression pattern of a novel gene encoding brain-fatty acid binding protein correlates with neuronal and glial Cell development[J]. Development 1994,120, 2637—2649.
[24] Bain, L.J. cDNA cloning, sequencing, and differential expression of a heart-type fatty acid-binding protein in the mummichog (Fundulus heteroclitus) [J]. Mar. Environ. Res. 2002, 54, 379—383.
[25] Matarese, V., Stone, R.L., Waggoner, D.W. & Bernlohr, D.A. Intracellular fatty acid trafficking and the role of cytosolic lipid binding proteins[J]. Prog. Lipid Res. 1989, 28, 245— 272.
[26] Zhang, J. & Haunerland, N.H. Transcriptional regulation of FABP expression in flight muscle of the desert locust, Schistocerca gregaria[J]. Insect Biochem. Mol. Biol. 1998, 28, 683-691.
[27] Vayda, M.E., Londravelli. R.L., Cashon, R.E., Costello, L. & Sidell, B.D. Two distinct types of fatty acid-binding protein are expressed in heart ventricle of Antarctic teleost fishes[J]. Biochem. J. 1998, 330, 375—382.
[28] Selman, K., Wallace, R.A., Sarka, A. & Qi, X. Stages of ocyte development in the zebra fish, Brachydanio rerio[J]. J. Morph. 1993, 218, 203—224.
[29] Denovan-Wright, E.M., Pierce, M. & Wright, J.M. Nucleotide sequence of cDNA clones coding for a brain-type fatty acid binding protein and its tissue-specific expression in adult zebra fish (Danio rerio) [J]. Biochim. Biophys. Acta. 2000,1492, 221—226.
[30] Pierce, M., Wang, Y., Denovan-Wright, E.M. & Wright, J.M. Nucleotide sequence of a cDNA clone coding for an intestinal-type fatty acid binding protein and its tissue-specific expression in zebra fish (Danio rerio) [J]. Biochim. Biophys. Acta. 2000,1490,175—183.
[31] Denovan Wright, E.M., Pierce, M., Sharma, M.K. & Wright, J.M. cDNA sequence and tissue-specific expression of a basic liver-type fatty acid binding protein in adult zebra fish (Danio rerio) [J]. Biochip. Biophys. Acta 2000, 1492, 227—232.
[32] Frank G S ,et al. Fatty acid-binding protein in the heart [J]. Molecular and Cellular Biochemistry, 1998, 180: 43—51.
[33] Kevin P C , et al. Cloning and tissue distribution of rat heart fatty acid binding protein mRNA: identical forms in heart and skeletal muscle [J]. Biochemistry, 1987, 26 (24):7900— 7904.
[34] Roger Peeter ,et al. Cloning of the cDNA encoding human skeletal2muscle fatty acid binding protein, its peptide sequence and chromosomal localization [J]. Biochem J, 1991, 276:203—207.
[35] Gerbens F, et al. Characterization, chromosomal localization, and genetic variation of the porcine heart fatty acid-binding protein gene [J]. Mammalian genome, 1997, 8: 328—332.
[36] Gerbens F, Verburg F J.- A dimorphic microsatellite in the porcine H-FABF gene at chromosome 6 [J]. Animal Genetics, 1998, 29, 398—413.
[37] Gerbens F , Jansen A. Effect of genetic variants of the heart fatty acid binding protein gene on intramuscular fat and performance traits in pigs [J]. J Animal Sci ,1999 ,77, 846—852.
[38] Gerbens F ,Verburg F J ,Van Moerkerk H T B ,et al. Associations of heart and adiposity fatty acid-binding protein gene expression with intramuscular fat content in pigs. [J]. J Anim Sci ,2002 ,92, 759—804.
[39] Gerbens F, Jansen A, Van Erp A J. The adiposity fatty acid-binding protein locus: characterization and association with intramuscular fat content in pigs [J].Mamma Genome, 1998, 9(12):1022—1026.
[40] FisherR M, Eriksson P, Hoffstedt J, HotamisligilG S, ThorneA, RydenM, HamstenA, ArnerP. Fatty acid binding protein expression in different adipose tissue depots from lean and obese individuals[J]. Diabetologia Oct, 2001, 44(10): 1268—1273.
[41] Prinsen C F, DeBruijnD R, MerkxG F, Veerkamp JH. Assign-ment of the human adiposity fatty acid binding protein gene(FABP4) to chromosome 8q~(21) using somatic cellhybrid and fluorescence in situ hybridization techniques[J].Genomics,1997,40:207-209.
[42]Brock Mann G,Timtchenko D,Das P,Renne U,Freyer G,Kuhla S,Teuscher F,Wolf J,Kuhn C,Schwerin M.Detection of QTL for bodyweight and body fat content in mice using genetic markers[J].Anim Breed Genet,1996,113,373-379.
[43]YEMan-Hong.Clone of gallus fatty acid binding protein gene and their relationship with intramuscular fat[J].Animal Science,2003,118,348-354..
[44]叶满红,鸡脂肪酸结合蛋白基因的克隆及其与肌内脂肪的关系[D].中国农业科学院博士论文,2003.
[45]HertzelAV,Bennaars-EidenA,BernlohrD A.Increased lipolysis in transgenic animals over expressing the epithelial fatty acid binding protein in adipose cells[J].LipidRes,2002,43(12):2105-2111.
[46]Boord JB,MaedaK,MakowskiL,BabaevV R,Fazio S,LintonM F,HotamisligilG S.Adipocyte fatty acid-binding protein,aP2,alters late atherosclerotic lesion formation in severe hypercholesterolemia[J].Arterioscler Thromb Vasc Biol,2002,22(10):1686-1691.
[47]BernlohrD A,AngusCW,LaneM D,BolanowskiM A,KellyT J Jr.Expression of specificmRNAs during adipose differentiation:identification of an mRNA encoding a homologue of myelin P2 protein[J].ProcNatlAcad Sci,1984,81(17):5468-5472.
[48]Spiegel man BM,Frank M,Green H.Molecular cloning of mRNA from 3T3 adiposities.Regulation of mRNA content for glycerophosphate dehydrogenase and other differentiation dependent proteins during adiposity development[J].BiolChem,1983,258(16):10083-10088.
[49]姜延志,李学伟.猪脂肪细胞型脂肪酸结合蛋白基因Bsm Ⅰ位点多态性与肌内脂肪含量的相关研究[J].中国畜牧杂志,2006,42(7):12-15.
[50]S.M.Di Pietro,J.H.Veerkamp,J.A.Santome,Isolation,amino acid sequence determination and binding properties of two fatty acid-binding proteins from axolotl (Ambistoma mexicanum) liver[J].Euro.J.Biochem.1999,259,127-134.
[51]R.M.Kaikus,W.K.Chan,R.R.Oritx de Montellano,N.M.Bass,Mechanisms of regulation of liver fatty acid-binding protein[J].Mol.Cell.Biochem.1993,123,93-100.
[52]F.Schroeder,J.R.Jefferson,D.Powell,S.Incerpi,J.K.Woodford,S.M.Colles,S.Myers-Payne,T.Emge,T.Hubbell,D.Moncecchi,D.R.Prows,C.E.Heyliger,Expression of rat L-FABP in mouse fibroblasts:role in fat absorption[J].Mol.Cell.Biochem.1993,123,73-83,
[53]R.G.H.J.Maatman,E.M.A.van de Westerlo,T.H.M.S.M.van Kuppevelt,J.H.Veerkamp,Molecular identification of the liver and the heart-type fatty acid-binding proteins in human and rat kidney[J].Biochem.J.1992,288,285-290.
[54]F.Ceciliani,H.L.Monaco,S.Ronchi,L.Faotto,P.Spadon,The primary structure of a basic(pI 9.0) fatty acid-binding protein from liver of Gallus domestics[J].Comp.Biochem. Physiology.1994,109B,261-271.
[55]C.H.Schleicher,J.A.Santome,Purification,characterization,and partial amino acid sequencing of an amphibian liver fatty acid binding protein[J].Biochem.Cell Biol.1996,74,109-115.
[56]S.M.Di Pietro,E.C.Dell' Angelica,C.H.Schleicher,J.A.Santome,Purification and structural characterization of a fatty acid-binding protein from the liver of the catfish Rhamdia sapo[J].Comp.Biochem.Physiol.B Biochem.Mol.Biol.1996,113,503-509.
[57]S.M.Di Pietro,E.C.Dell' Angelica,J.H.Veerkamp,N.Sterin Spiziale,J.A.Santome",Amino acid sequence,binding properties and evolutionary relationships of the basic liver fatty-acid-binding protein from the catfish Rhamdia sapo[J].Euro.J.Biochem.1997,249,510-517.
[58]Veerkamp JH & Maatman RGHJ.Cytoplasmic fatty acid binding proteins:their structure and genes[J].Progress in Lipid Research,1995,34,17-52
Zanotti G,Scapin G,Spadon P,et al.Three-dimensional structure of recombinant human muscle fatty acid-binding protein[J].J Biol Chem,1992,267(26):18541-18550
[59]Giuseppe Zanotti.Muscle fatty acid-binding protein[J].Biochim.Biophys,1999,1441:94-105
[60]Becket MM,Kalinna BH,Waine GJ,et al.Gene cloning,overproduction and purification of a functionally active cytoplasmic fatty acid-binding protein from the human blood fluke Schistosoma japonicum[J].Gene,1994,148(2):321-325
[61]Guor Mour Her,Yang-Hui Yeh,Jen-Leih Wu.Functional Conserved Elements Mediate Intestinal-Type Fatty Acid Binding Protein(I-FABP) Expression in the Gut Epithelia of Zebra fish Larvae[J].Developmental Dynamics,2004,230,734-742.
[62]Rong-Zong Liu,Eileen M Denovan-Wright,Jonathan M Wright.Structure,linkage mapping and expression of the heart-type fatty acid-binding protein gene(fabp3) from zebra fish(Danio rerio)[J].Journal of Europe Biochemical,2003,270,3223-3234.
[63]Santiago M Di Pietro,Jose A Santome.Structural and Biochemical Characterization of the Lungfish(Lepidosiren paradoxa) Liver Basic Fatty Acid Binding Protein[J].Archives of Biochemistry and Biophysics,2001,388:81-90.
[64]白亮,庞卫军,杨公社.烟酰胺在原代培养的猪前体脂肪细胞增殖分化中的作用[J].动物学报,2007,53(6):1063-1068.
[65]Mukesh K Sharmaa,Eileen M Denovan-Wrightb,Agnes Degravec,et al.Sequence,linkage mapping and early developmental expression of the intestinal-type fatty acid-binding protein gene(fabp2) from zebra fish[J].Comparative Biochemistry and Physiology:Part B,2004,138,391-39.
[66]王启贵,李宁,邓学梅,等.鸡细胞外脂肪酸结合蛋白基因编码区的单核苷酸多态与腹 脂性状的相关研究[J].自然科学进展.2002,12(4):420-422.
[67]Gerben F D,Koning D J,Harders F L,et al.Effect of genetic variants of the hear-t fatty acid binding protein gene on intransmuscular fat and performance traits in pig[J].Journal of Animal Science,1999,77,846-852.
[68]F.Gerbens,叶满红.猪心脏和脂肪型脂肪酸结合蛋白基因表达与肌内脂肪含量的关系[J].国外畜牧科技,2002,29(1):432-437.
[69]敖金霞,李辉,王启贵,等.鹅生长激素基因克隆和组织表达[J].农业生物技术学报,2004,12(5):552-555.
[70]Daniels C,Noy N,and Zakim D.Rates of hydration of fatty acids bound to unilamellar vesicles of phosphatidylcholine or to albumin[J].Biochemistry,1985,24(13):3286-3292
[71]Hertzel AV,Bennaars-Eiden A,Bemlohr DA.Increased lipolysis in transgenic animals over expressing the epithelial fatty acid binding protein in adipose cells[J].Lipid Res,2002,43(12):2105-2111
[72]Sacchettini JC,Gordon JI.Rat intestinal fatty acid binding protein,A model system for annlyzing the forces that can bind fatty acids to proteins[J].Chemistry.1993;268:18399-18402
[73]董解菊.脂肪酸结合蛋白测定及临床应用研究进展[J].国外医学.临床生物化学与检验学分册2001;22,74-76
[74]张桂香,曹红鹤,王立贤,等.9个猪种H-FABP基因5′-上游区和第二内含子的遗传变异[J].畜牧兽医学报,2002,33(4):340-343.
[75]Descalzi C F,Dozin B,and Zerega B.Ex-FABP:a fatty acid binding lipocalin developmentally regulated in chicken endochondral bone formation and myogenesis [J].Biochemical & Biophysical Acta,2000,1482(12):127-135.
[76]Storch J,Thumser AE.The fatty acid transport function of fatty acid-binding proteins[J].molecule Cell Biol Lipid 2000;1486:28-44
[77]Kurian E,Kirk W R,Prendergast FG.Affinity of fatty acid for rat intestinal fatty acid binding protein:Further examination[J].Biochemistry 1996;35:3865-3874
[78]Richieri GV,Low PJ,Ogata RT,Kleinfeld AM.Thermody-namics of fatty acid binding to engineered mutants of theadipocyte and intestinal fatty acid-binding proteins[J].BiolChem 1998,273,7397-7405
[79]Ockner RK,Manning Ja.Fatty acid-binding protein insmallintestine.Identification,isolation,and evidence for its role in cellular fattv acid transport[J].Lipids 1996,31,5223-5227
[80]Kaikaus RM,Chan W K,Ortiz de M ontellano PR,Bass NM.Mechanisms of regulation of liver fatty acid-binding protein[J].M of Cell Biochem 1993,123,93-100
[81]Zimmerman AW,van Moerkerk HT,Veerkamp JH.Ligandspecificitv and conform ational stability of hum an fatty acid-binding proteins[J].Biochem Cell Biol 2001,33,865-876
[82] Stewart JM , Driedzic WR, Berkelaar Ja. Fatty acid-binding protein facilitates the diffusion of oleate in a m odel cytosolsystem[J]. Biochemistry, 1991, 275, 569-573
[83] Wootan MG, Bernlohr DA, Storch T. Mechanism of fluorescent fatty acid trans fer from adiposity fatty acid binding protein to membranes[J]. Biochemistry 1993, 32, 8622—8627
[84] Gerbens F,De Koning D J,Harders F L, et al.The effect of adiposity and heart fatty acid-binding protein genes on intramuscular fat and back fat content in Meishan crossbed pigs[J].Anim Sci,2000,78 (3):552-529.
[85] Gerbens F,Van Erp A J,Harders F L, et al.Effect of genetic variants of the heart fatty acid-binding protein gene on intramuscular fat and performance traits in pigs[J].Anima Sci,1999,77(4):846-852.
[86] Peeters RA, Veerkamp JH, Demel RA. Are fatty acid-binding proteins involved in fatty acid transfer [J] Biochem. Biophys, 1989a, 1002(1):8—13
[87] Ockner RK, Manning JA, and Kane JP. Fatty acid binding protein. Isolation from rat liver, characterization, and immunochemical quantification [J]. J. Biol. Chem., 1982, 257, 7872— 7878.
[88] Roth KA, Hertz JM, Gordon JI. Mapping enter endocrine cell populations in transgenic mice reveals an unexpected degree of complexity in cellular differentiation within the gastrointestinal tract [J]. J cell Bile, 1990,100,1791—1801
[89] Clarke SD, Armstrong MK. Cellular lipid binding proteins: expression, function, and nutritional regulation [J]. FASEB J, 1989, 3(13): 2480—2487
[90] 林万华,黄路生,任军,等. 中外十个猪种H-FABP基因遗传变异的研究[J]. 遗传学报, 2002, 29(1): 12-15
[91] Distel R. J. et al. Fatty acid regulation of gene expression. [J]. J Bio Chem. 1992, 267, 5937—5941.
[92] Santiago M. Di Pietro, Jose' A. Santome'. Structural and Biochemical Characterization of the Lungfish (Lepidosiren paradoxa) Liver Basic Fatty Acid Binding Proteinl [J]. Archives of Biochemistry and Biophysics, 2001, 388, 81—90
[93] Mukesh K. Sharmaa, Eileen M. Denovan-Wrightb, Agnes Degravec, et al. Sequence, linkage mapping and early developmental expression of the intestinal-type fatty acid-binding protein gene (fabp2) from zebra fish[J]. Comparative Biochemistry and Physiology, 2004,Part B 138,391-395
[94] Thompson J D, Gibson T J, Plewniak F, et al. The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools [J]. Nucleic Acids Res, 1997, 24, 4876—4882.
[95] Saitou, Nei. The neighbor-joining method: A new method for reconstructing phylogenetic trees [J]. Molecular Biology and Evolution, 1987, 4, 406—425
[96] Felsenstein J. The troubled growth of statistical phylogenetics [J]. Syst Biol, 2001, 50(4) :465 -467.
[97] E.M. Denovan-Wright, M. Pierce, J.M. Wright, Nucleotide sequence of cDNA clones coding for a brain-type fatty acid binding protein and its tissue-specific expression in adult zebra fish (Danio rerio), Biochim. Biophys. Acta 2000, 1492, 223-228.
[98] S.M. Di Pietro, E.C. Dell'Angelica, C.H. Schleicher, J.A. Santome¨, Purification and structural characterization of a fatty acid-binding protein from the liver of the catfish Rhamdia sapo[J]. Comp. Biochem. Physiology. B Biochem. Mol. Biol. 1996,113 ,503-509.
[99] S.M. Di Pietro, E.C. Dell' Angelica, J.H. Veerkamp, N. Sterin-Spiziale, J.A. Santome, Amino acid sequence, binding properties and evolutionary relationships of the basic liver fatty-acid-binding protein from the catfish Rhamdia sapo[J]. Eur. J. Biochem. 1997, 249, 510-517.
[100] Sweetser DA, Hauft SM, Hoppe PC, Birkenmeier EH, Gordon JI. Transgenic mice containing intestinal fatty acid binding protein-human growth hormone fusion genes exhibit corrected regional and cell-specific expression of the reporter gene in their small intestine[J]. Proc. Natl. Acad. Sci. USA.1988, 85(24): 9611—9615
[101] Jones PD, Carne A, Bass NM, Grigor MR. Isolation and characterization of fatty acid binding proteins from mammary tissue of lactating rats[J]. Biochemistry. J.1988, 251(3): 919- 925
[102] Bass, N. M. et al, Tissue expression of three structurally different fatty acid binding proteins from rat heart muscle, liver, and intestine[J]. Biochem. Biol.Res. Commun. 1986, 137, 929-935
[103] Bass NM, Manning JA, Ockner RK, Gordon JI, Seetharam S, Alpers DH. Regulation of the biosynthesis of two distinct fatty acid-binding proteins in rat liver and intestine. Influences of sex difference and of clofibrate[J]. J. Biol.Chem. 1985a, 260(3): 1432—1436
[104] Paulussen RJ, van Moerkerk HT, Veerkamp JH. Immunochemical quantitation of fatty acid-binding proteins. Tissue distribution of liver and heart FABP types in human and porcine tissues[J]. Int. J. Biochem. 1990, 22(4): 393—398
[105] Bass NM, Manning JA, Ockner RK. Turnover and short-term regulation of fatty acid binding protein in fiver[J]. J. Biol.Chem. 1985b, 260(17): 9603—9607
[106] Sheridan M, Wilkinson TC, Wilton DC. Studies on fatty acid-binding proteins. Changes in the concentration of hepatic fatty acid-binding protein during development in the rat[J]. Biochem. J.1987, 42(3): 919—922
[107] Levin MS, Pitt AJ, Schwartz AL, Edwards PA, Gordon JI. Developmental changes in the expression of genes involved in cholesterol biosynthesis and lipid transport in human and rat fetal and neonatal livers[J]. Biochem. Biop s. Acta .1989, 1003(3): 377-382.
[108] Norbert H, Haunerland, et al; Fatty acid-binding proteins-insights from genetic manipulations[J]. Progress in Lipid Research, 2004, 43: 328—349
[109]Marti A,et al,Down-regulation of heart HFABP and UCP2 gene expression in diet-induced(cafeteria) obese rats[J].J.Physiol.Biochem.2002,58(2):69-74
[110]Kang K,et al,CLA inhibits differentiation of adiposities and decreases PPARgamma expression[J].Biochem.Biophys.Res.Commun.2003,303(3):795-799
[111]Londraville RL,Duvall CS.Murine leptin injections increase intracellular fatty acid-binding protein in green sunfish(Lepomis cyanellus)[J].Gen.Comp.Endocrinol.2002,129(1):56-62
[112]Piro S,Anello M,Di Pietro C,Lizzio MN,Patane G,Rabuazzo AM,Vigneri R,Purrello M,Purrello F.Chronic exposure to free fatty acids or high glucose induces apoptosis in rat pancreatic islets:possible role of oxidative stress[J].Metabolism.2002,51(10):1340-1347
[113]Glatz JF,et al,Diurnal variation of cytosofic fatty acid binding protein content and of palinitate oxidation in rat fiver and heart[J].J.Biol.Chem.1984,259(7):4295-4300
[114]Kontani Y,et al,Effects of aging and denervation on the expression of uncoupling proteins in slow and fast-twitch muscles of rats[J].J.Biochemistry.2002,132(2):309-315
[115]Clavel S,Farout L,Briand M,Briand Y,Jouanel P.Effect of endurance training and/or fish oil supplemented diet on cytoplasmic fatty acid binding protein in rat skeletal muscles and heart[J].Euro.J.Appl.Physiology.2002,87(3):193-201
[116]Veerkamp JH,van Moerkerk HT.Fatty acid binding protein and its relation to fatty acid oxidation[J].Mol.Cell Biochem.1993,123(2):101-106
[117]Maeda K,Uysal KT,Makowski L Gorgun CZ,Atsumi G,Parker RA,Bruning J,Hertzel AV,Bernlohr DA,Hotamisligil GS.Role of the fatty acid binding protein mall in obesity and insulin resistance[J].Diabetes;2003,52(2):300-307
[118]韩为东,于力.RACE技术在钓取白血病相关基因LRP16全长cDNA中的应用[J].中国实验血液科学杂志,2001,9(1):18-21
[119]王锋超,高京生.由EST到全长cDNA-RACE及相关技术进展[J].生命的化学,2002,22(3):294-297
[120]张庆华,茅矛.基因组研究中全长cDNA克隆的策略[J].生物工程进展,2000,20(4):3-5.
[121]杜占文,刘立仁.用RACE结合cDNA文库筛选的方法获取新的锌指蛋白基因[J].遗传,2002,24(3):329-331.
[122]邱为民,张思仲.一种新的cDNA末端快速扩增获取全长cDNA的方法[J].遗传,2001,23(5):480-482.
[123]萨姆布鲁克,弗里奇,曼里阿蒂斯.分子克隆实验指南(第二版)[M]。金冬雁,黎阵枫,侯云德.译.北京:科学出版社,1998,16-33.
[124]ZHANG Yong,ZHANG Wei-Min,LI Xin,et al.Construction of cDNA Library from the Ovary of Epinephelus coiodes and Cloning of a Fatty Acid Binding Protein(FABP)[J].Acta Scientiarun Naturalium Univerisitatis Sunyatseni,2003,42(2):66-69.
[125]Santiago M Di Pietro,Jose A Santome.Structural and Biochemical Characterization of the Lungfish(Lepidosiren paradoxa) Liver Basic Fatty Acid Binding Protein[J].Archives of Biochemistry and Biophysics,2001,388,81-90.
[126]黄玉海,汪以真,单体中,等.猪心型脂肪酸结合蛋白基因的克隆及不同生长阶段表达的差异[J].农业生物技术学报,2006,14(5):657-661.
[127]Mukesh K Sharmaa,Eileen M Denovan-Wrightb,Agnes Degravec,et al.Sequence,l-inkage mapping and early developmental expression of the intestinal-type fatty acid-binding protein gene(fabp2) from zebra fish[J].Comparative Biochemistry and Physiology:Part B,2004,138,391-399.
[128]敖金霞.鹅GH和A-FABP基因的克隆及基因多态性与鸡生长和体组成性状的相关研究[D].哈尔滨:东北农业大学,2003.
[129]黄河,帅素容,余先琼,等.藏猪心脏脂肪酸结合蛋白基因(H-FABP)组织表达差异性研究[J].四川农业大学学报,2007,25(4):480-483.
[130]林万华,黄路生,任军,等.H-FABP基因型对二花脸猪相关性状影响的初步分析[J],畜牧兽医学报,2003,34(4):318-324.
[131]李惠侠,杨公社,卢建雄.花生四烯酸对大鼠前体脂肪细胞生长与分化的影响[J].中国生物化学与分子生物学报,2005,06,27-31.
[132]Gerben F D,Koning D J,Harders F L,et al.Effect of genetic variants of the hear-t fatty acid binding protein gene on intransmuscular fat and performance traits in pig[J].Journal of Animal Science,1999,77:846-852.
[133]孟和,王桂华,王启贵,等.鸡PPAR基因单核苷酸多态与脂肪性状的相关研究[J].遗传学报,2002,29(2):119-123.
[134]Rong-Zong Liu,Eileen M Denovan-Wright Jonathan M Wright.Structure,mRNA ex-pression and linkage mapping of the brain-type fatty acid-binding protein gene(fabp7) from zebra fish(Danio rerio)[J].Journal of Europe Biochemical.,2003,270:715-725.
[135]Guor Mour Her,Yang-Hui Yeh,Jen-Leih Wu.Functional Conserved Elements Media-te Intestinal-Type Fatty Acid Binding Protein(I-FABP) Expression in the Gut Epithelia of Zebra fish Larvae[J].Developmental Dynamics,2004,230:734-742.
[136]Rong-Zong Liu,Eileen M Denovan-Wright,Jonathan M Wright.Structure,linkage mapping and expression of the heart-type fatty acid-binding protein gene(fabp3) from zebra fish(Danio rerio)[J].Journal of Europe Biochemical,2003,270,3223-3234.
[137]Eileen M Denovan Wright,Marianne Pierce,Mukesh K Sharma,et al.cDNA sequence and tissue-specific expression of a basic liver-type fatty acid binding protein adult zebra fish (Danio rerio)[J].Biochemical & Biophysical Acta,2000,1492,227-232.
[2]Eileen M Denovan Wright,Marianne Pierce,Mukesh K Sharma,et al.cDNA sequence and tissue-specific expression of a basic liver-type fatty acid binding protein in adult zebra fish (Danio rerio)[J].Biochemical & Biophysical Acta,2000,1492:227-232.
[3]庞卫军,白亮,杨公社.西部地区主要猪种H-FABP基因多态性,IMF含量及不同基因型脂肪细胞脂[J].遗传学报,2002,33(6):515-524.
[4]黄治国,熊俐,刘振山,等.绵羊肌肉H-FABP和PPARγ基因表达的发育性变化及其对肌内脂肪含量的影响[J].遗传学报,2002,33(6):507-514.
[5]Santiago M Di Pietro,Jose A Santome.Structural and Biochemical Characterization of the Lungfish(Lepidosiren paradoxa) Liver Basic Fatty Acid Binding Protein[J].Archives of Biochemistry and Biophysics,2001,388:81-90.
[6]庞卫军,杨公社.8个猪种和野猪H-FABP基因分子标记与IMF含量的关系[J].农业生物技术学报,2005,13(6):733-738.
[7]王启贵,李宁,邓学梅,等.鸡细胞外脂肪酸结合蛋白基因单核苷酸多态性与腹脂性状的相关研究[J].中国科学(C辑),2001,31(3):266-270.
[8]Gentili C.Cemetfi S.Tacchei C et al Expression of the extracellular fatty binding protein during muscle fiber formation,Exp Cell Res,1998,24:410-418.
[9]Cancedda F D,Malpet[M],Gentili C,et al The developmentally regulated avian Chil Jpocalin is an extracetlul as fatty acid-binding protein J Biol Chem.1996.377{J0):633-638
[10]Gerhen F Harders F VeeTkamp J Effect of genetic variant of bean fatty acid binding protein gene on intramuscular fat and performance traits in pig[J].Animal Science.1999,77:846-852
[11]王启贵,李宁,邓学梅,等.鸡脂肪酸结合蛋白基因的克隆和测序分析[J].遗传学报,2002,29(2):115-118.
[12]赵春江,李宁,邓学梅.AS-PCR技术检测鸡EX-FABP基因型方法的建立[J].农业生物技术学报,2003,11(3):273-275.
[13]庞卫军,杨公社,龙火生,等.猪心脏脂肪酸结合蛋白基因PCR-RFLP分子标记研究[K].生物技术通讯,2004,15(2):124-127.
[14]Eileen M.Denovan-Wright,Marianne Pierce,Mukesh K.Sharma,et al.cDNA sequence and tissue-specific expression of a basic liver-type fatty acid binding protein in adult zebra fish (Danio rerio)[J].Biochimica & Biophysical Acta,2000,1492:227-232
[15]Ockner RK,Manning JA,and Kane JP.Fatty acid binding protein.Isolation from rat liver, characterization, and immunochemical quantification [J]. J. Biol. Chem., 1982, 257: 7872— 7878.
[16]Santiago M. Di Pietro, Jose A. Santome. Structural and Biochemical Characterization of the Lungfish (Lepidosiren paradoxa) Liver Basic Fatty Acid Binding Proteinl [J]. Archives of Biochemistry and Biophysics, 2001, 388: 81—90
[17] Maatman RG, van de Westerlo EM, van Kuppevelt TH, et al. Molecular identification of the liver and the heart-type fatty acid-binding proteins in human and rat kidney. Use of the reverse transcriptase polymerase chain reaction [J]. Biochem J, 1992, 288 (1): 285-290.
[18] Guor Mour Her, Yang-Hui Yeh, Jen-Leih Wu. Functional Conserved Elements Media-te Intestinal-Type Fatty Acid Binding Protein (I-FABP) Expression in the Gut Epithel-ia of Zebra fish Larvae[J]. Developmental Dynamics, 2004, 230: 734—742
[19] Gerbens F,De Koning D J,Harders F L, et al.The effect of adiposity and heart fatty acid-binding protein genes on intramuscular fat and back fat content in Meishan crossbed pigs[J].Anima Sci,2000,78 (3): 552—529.
[20] Hertzel AV, Bennaars-Eiden A, Bemlohr DA.Increased lipolysis in transgenic animals over expressing the epithelial fatty acid binding protein in adipose cells [J]. Lipid Res, 2002, 43(12): 2105—2111
[21] Bass, N.M. & Manning, J.A. Tissue expression of three structurally different fatty acid binding proteins from rat heart muscle, liver, and intestine[J]. Biochem. Biophys. Res. Commun. 1986,137, 929—935.
[22] Paulussen, R.J.A., van Moerkerk, H.T.B. & Veerkamp, J.H. Immunochemical quantitation of fatty acid-binding proteins. Tissue distribution of liver and heart FABP types in human and porcine tissues[J]. Int. J. Biochem. 1990, 22, 393—398.
[23] Kurtz, A., Zimmer, A., Schnu tgen, F., Bruning, G., Spener, F . & Muller, T. The expression pattern of a novel gene encoding brain-fatty acid binding protein correlates with neuronal and glial Cell development[J]. Development 1994,120, 2637—2649.
[24] Bain, L.J. cDNA cloning, sequencing, and differential expression of a heart-type fatty acid-binding protein in the mummichog (Fundulus heteroclitus) [J]. Mar. Environ. Res. 2002, 54, 379—383.
[25] Matarese, V., Stone, R.L., Waggoner, D.W. & Bernlohr, D.A. Intracellular fatty acid trafficking and the role of cytosolic lipid binding proteins[J]. Prog. Lipid Res. 1989, 28, 245— 272.
[26] Zhang, J. & Haunerland, N.H. Transcriptional regulation of FABP expression in flight muscle of the desert locust, Schistocerca gregaria[J]. Insect Biochem. Mol. Biol. 1998, 28, 683-691.
[27] Vayda, M.E., Londravelli. R.L., Cashon, R.E., Costello, L. & Sidell, B.D. Two distinct types of fatty acid-binding protein are expressed in heart ventricle of Antarctic teleost fishes[J]. Biochem. J. 1998, 330, 375—382.
[28] Selman, K., Wallace, R.A., Sarka, A. & Qi, X. Stages of ocyte development in the zebra fish, Brachydanio rerio[J]. J. Morph. 1993, 218, 203—224.
[29] Denovan-Wright, E.M., Pierce, M. & Wright, J.M. Nucleotide sequence of cDNA clones coding for a brain-type fatty acid binding protein and its tissue-specific expression in adult zebra fish (Danio rerio) [J]. Biochim. Biophys. Acta. 2000,1492, 221—226.
[30] Pierce, M., Wang, Y., Denovan-Wright, E.M. & Wright, J.M. Nucleotide sequence of a cDNA clone coding for an intestinal-type fatty acid binding protein and its tissue-specific expression in zebra fish (Danio rerio) [J]. Biochim. Biophys. Acta. 2000,1490,175—183.
[31] Denovan Wright, E.M., Pierce, M., Sharma, M.K. & Wright, J.M. cDNA sequence and tissue-specific expression of a basic liver-type fatty acid binding protein in adult zebra fish (Danio rerio) [J]. Biochip. Biophys. Acta 2000, 1492, 227—232.
[32] Frank G S ,et al. Fatty acid-binding protein in the heart [J]. Molecular and Cellular Biochemistry, 1998, 180: 43—51.
[33] Kevin P C , et al. Cloning and tissue distribution of rat heart fatty acid binding protein mRNA: identical forms in heart and skeletal muscle [J]. Biochemistry, 1987, 26 (24):7900— 7904.
[34] Roger Peeter ,et al. Cloning of the cDNA encoding human skeletal2muscle fatty acid binding protein, its peptide sequence and chromosomal localization [J]. Biochem J, 1991, 276:203—207.
[35] Gerbens F, et al. Characterization, chromosomal localization, and genetic variation of the porcine heart fatty acid-binding protein gene [J]. Mammalian genome, 1997, 8: 328—332.
[36] Gerbens F, Verburg F J.- A dimorphic microsatellite in the porcine H-FABF gene at chromosome 6 [J]. Animal Genetics, 1998, 29, 398—413.
[37] Gerbens F , Jansen A. Effect of genetic variants of the heart fatty acid binding protein gene on intramuscular fat and performance traits in pigs [J]. J Animal Sci ,1999 ,77, 846—852.
[38] Gerbens F ,Verburg F J ,Van Moerkerk H T B ,et al. Associations of heart and adiposity fatty acid-binding protein gene expression with intramuscular fat content in pigs. [J]. J Anim Sci ,2002 ,92, 759—804.
[39] Gerbens F, Jansen A, Van Erp A J. The adiposity fatty acid-binding protein locus: characterization and association with intramuscular fat content in pigs [J].Mamma Genome, 1998, 9(12):1022—1026.
[40] FisherR M, Eriksson P, Hoffstedt J, HotamisligilG S, ThorneA, RydenM, HamstenA, ArnerP. Fatty acid binding protein expression in different adipose tissue depots from lean and obese individuals[J]. Diabetologia Oct, 2001, 44(10): 1268—1273.
[41] Prinsen C F, DeBruijnD R, MerkxG F, Veerkamp JH. Assign-ment of the human adiposity fatty acid binding protein gene(FABP4) to chromosome 8q~(21) using somatic cellhybrid and fluorescence in situ hybridization techniques[J].Genomics,1997,40:207-209.
[42]Brock Mann G,Timtchenko D,Das P,Renne U,Freyer G,Kuhla S,Teuscher F,Wolf J,Kuhn C,Schwerin M.Detection of QTL for bodyweight and body fat content in mice using genetic markers[J].Anim Breed Genet,1996,113,373-379.
[43]YEMan-Hong.Clone of gallus fatty acid binding protein gene and their relationship with intramuscular fat[J].Animal Science,2003,118,348-354..
[44]叶满红,鸡脂肪酸结合蛋白基因的克隆及其与肌内脂肪的关系[D].中国农业科学院博士论文,2003.
[45]HertzelAV,Bennaars-EidenA,BernlohrD A.Increased lipolysis in transgenic animals over expressing the epithelial fatty acid binding protein in adipose cells[J].LipidRes,2002,43(12):2105-2111.
[46]Boord JB,MaedaK,MakowskiL,BabaevV R,Fazio S,LintonM F,HotamisligilG S.Adipocyte fatty acid-binding protein,aP2,alters late atherosclerotic lesion formation in severe hypercholesterolemia[J].Arterioscler Thromb Vasc Biol,2002,22(10):1686-1691.
[47]BernlohrD A,AngusCW,LaneM D,BolanowskiM A,KellyT J Jr.Expression of specificmRNAs during adipose differentiation:identification of an mRNA encoding a homologue of myelin P2 protein[J].ProcNatlAcad Sci,1984,81(17):5468-5472.
[48]Spiegel man BM,Frank M,Green H.Molecular cloning of mRNA from 3T3 adiposities.Regulation of mRNA content for glycerophosphate dehydrogenase and other differentiation dependent proteins during adiposity development[J].BiolChem,1983,258(16):10083-10088.
[49]姜延志,李学伟.猪脂肪细胞型脂肪酸结合蛋白基因Bsm Ⅰ位点多态性与肌内脂肪含量的相关研究[J].中国畜牧杂志,2006,42(7):12-15.
[50]S.M.Di Pietro,J.H.Veerkamp,J.A.Santome,Isolation,amino acid sequence determination and binding properties of two fatty acid-binding proteins from axolotl (Ambistoma mexicanum) liver[J].Euro.J.Biochem.1999,259,127-134.
[51]R.M.Kaikus,W.K.Chan,R.R.Oritx de Montellano,N.M.Bass,Mechanisms of regulation of liver fatty acid-binding protein[J].Mol.Cell.Biochem.1993,123,93-100.
[52]F.Schroeder,J.R.Jefferson,D.Powell,S.Incerpi,J.K.Woodford,S.M.Colles,S.Myers-Payne,T.Emge,T.Hubbell,D.Moncecchi,D.R.Prows,C.E.Heyliger,Expression of rat L-FABP in mouse fibroblasts:role in fat absorption[J].Mol.Cell.Biochem.1993,123,73-83,
[53]R.G.H.J.Maatman,E.M.A.van de Westerlo,T.H.M.S.M.van Kuppevelt,J.H.Veerkamp,Molecular identification of the liver and the heart-type fatty acid-binding proteins in human and rat kidney[J].Biochem.J.1992,288,285-290.
[54]F.Ceciliani,H.L.Monaco,S.Ronchi,L.Faotto,P.Spadon,The primary structure of a basic(pI 9.0) fatty acid-binding protein from liver of Gallus domestics[J].Comp.Biochem. Physiology.1994,109B,261-271.
[55]C.H.Schleicher,J.A.Santome,Purification,characterization,and partial amino acid sequencing of an amphibian liver fatty acid binding protein[J].Biochem.Cell Biol.1996,74,109-115.
[56]S.M.Di Pietro,E.C.Dell' Angelica,C.H.Schleicher,J.A.Santome,Purification and structural characterization of a fatty acid-binding protein from the liver of the catfish Rhamdia sapo[J].Comp.Biochem.Physiol.B Biochem.Mol.Biol.1996,113,503-509.
[57]S.M.Di Pietro,E.C.Dell' Angelica,J.H.Veerkamp,N.Sterin Spiziale,J.A.Santome",Amino acid sequence,binding properties and evolutionary relationships of the basic liver fatty-acid-binding protein from the catfish Rhamdia sapo[J].Euro.J.Biochem.1997,249,510-517.
[58]Veerkamp JH & Maatman RGHJ.Cytoplasmic fatty acid binding proteins:their structure and genes[J].Progress in Lipid Research,1995,34,17-52
Zanotti G,Scapin G,Spadon P,et al.Three-dimensional structure of recombinant human muscle fatty acid-binding protein[J].J Biol Chem,1992,267(26):18541-18550
[59]Giuseppe Zanotti.Muscle fatty acid-binding protein[J].Biochim.Biophys,1999,1441:94-105
[60]Becket MM,Kalinna BH,Waine GJ,et al.Gene cloning,overproduction and purification of a functionally active cytoplasmic fatty acid-binding protein from the human blood fluke Schistosoma japonicum[J].Gene,1994,148(2):321-325
[61]Guor Mour Her,Yang-Hui Yeh,Jen-Leih Wu.Functional Conserved Elements Mediate Intestinal-Type Fatty Acid Binding Protein(I-FABP) Expression in the Gut Epithelia of Zebra fish Larvae[J].Developmental Dynamics,2004,230,734-742.
[62]Rong-Zong Liu,Eileen M Denovan-Wright,Jonathan M Wright.Structure,linkage mapping and expression of the heart-type fatty acid-binding protein gene(fabp3) from zebra fish(Danio rerio)[J].Journal of Europe Biochemical,2003,270,3223-3234.
[63]Santiago M Di Pietro,Jose A Santome.Structural and Biochemical Characterization of the Lungfish(Lepidosiren paradoxa) Liver Basic Fatty Acid Binding Protein[J].Archives of Biochemistry and Biophysics,2001,388:81-90.
[64]白亮,庞卫军,杨公社.烟酰胺在原代培养的猪前体脂肪细胞增殖分化中的作用[J].动物学报,2007,53(6):1063-1068.
[65]Mukesh K Sharmaa,Eileen M Denovan-Wrightb,Agnes Degravec,et al.Sequence,linkage mapping and early developmental expression of the intestinal-type fatty acid-binding protein gene(fabp2) from zebra fish[J].Comparative Biochemistry and Physiology:Part B,2004,138,391-39.
[66]王启贵,李宁,邓学梅,等.鸡细胞外脂肪酸结合蛋白基因编码区的单核苷酸多态与腹 脂性状的相关研究[J].自然科学进展.2002,12(4):420-422.
[67]Gerben F D,Koning D J,Harders F L,et al.Effect of genetic variants of the hear-t fatty acid binding protein gene on intransmuscular fat and performance traits in pig[J].Journal of Animal Science,1999,77,846-852.
[68]F.Gerbens,叶满红.猪心脏和脂肪型脂肪酸结合蛋白基因表达与肌内脂肪含量的关系[J].国外畜牧科技,2002,29(1):432-437.
[69]敖金霞,李辉,王启贵,等.鹅生长激素基因克隆和组织表达[J].农业生物技术学报,2004,12(5):552-555.
[70]Daniels C,Noy N,and Zakim D.Rates of hydration of fatty acids bound to unilamellar vesicles of phosphatidylcholine or to albumin[J].Biochemistry,1985,24(13):3286-3292
[71]Hertzel AV,Bennaars-Eiden A,Bemlohr DA.Increased lipolysis in transgenic animals over expressing the epithelial fatty acid binding protein in adipose cells[J].Lipid Res,2002,43(12):2105-2111
[72]Sacchettini JC,Gordon JI.Rat intestinal fatty acid binding protein,A model system for annlyzing the forces that can bind fatty acids to proteins[J].Chemistry.1993;268:18399-18402
[73]董解菊.脂肪酸结合蛋白测定及临床应用研究进展[J].国外医学.临床生物化学与检验学分册2001;22,74-76
[74]张桂香,曹红鹤,王立贤,等.9个猪种H-FABP基因5′-上游区和第二内含子的遗传变异[J].畜牧兽医学报,2002,33(4):340-343.
[75]Descalzi C F,Dozin B,and Zerega B.Ex-FABP:a fatty acid binding lipocalin developmentally regulated in chicken endochondral bone formation and myogenesis [J].Biochemical & Biophysical Acta,2000,1482(12):127-135.
[76]Storch J,Thumser AE.The fatty acid transport function of fatty acid-binding proteins[J].molecule Cell Biol Lipid 2000;1486:28-44
[77]Kurian E,Kirk W R,Prendergast FG.Affinity of fatty acid for rat intestinal fatty acid binding protein:Further examination[J].Biochemistry 1996;35:3865-3874
[78]Richieri GV,Low PJ,Ogata RT,Kleinfeld AM.Thermody-namics of fatty acid binding to engineered mutants of theadipocyte and intestinal fatty acid-binding proteins[J].BiolChem 1998,273,7397-7405
[79]Ockner RK,Manning Ja.Fatty acid-binding protein insmallintestine.Identification,isolation,and evidence for its role in cellular fattv acid transport[J].Lipids 1996,31,5223-5227
[80]Kaikaus RM,Chan W K,Ortiz de M ontellano PR,Bass NM.Mechanisms of regulation of liver fatty acid-binding protein[J].M of Cell Biochem 1993,123,93-100
[81]Zimmerman AW,van Moerkerk HT,Veerkamp JH.Ligandspecificitv and conform ational stability of hum an fatty acid-binding proteins[J].Biochem Cell Biol 2001,33,865-876
[82] Stewart JM , Driedzic WR, Berkelaar Ja. Fatty acid-binding protein facilitates the diffusion of oleate in a m odel cytosolsystem[J]. Biochemistry, 1991, 275, 569-573
[83] Wootan MG, Bernlohr DA, Storch T. Mechanism of fluorescent fatty acid trans fer from adiposity fatty acid binding protein to membranes[J]. Biochemistry 1993, 32, 8622—8627
[84] Gerbens F,De Koning D J,Harders F L, et al.The effect of adiposity and heart fatty acid-binding protein genes on intramuscular fat and back fat content in Meishan crossbed pigs[J].Anim Sci,2000,78 (3):552-529.
[85] Gerbens F,Van Erp A J,Harders F L, et al.Effect of genetic variants of the heart fatty acid-binding protein gene on intramuscular fat and performance traits in pigs[J].Anima Sci,1999,77(4):846-852.
[86] Peeters RA, Veerkamp JH, Demel RA. Are fatty acid-binding proteins involved in fatty acid transfer [J] Biochem. Biophys, 1989a, 1002(1):8—13
[87] Ockner RK, Manning JA, and Kane JP. Fatty acid binding protein. Isolation from rat liver, characterization, and immunochemical quantification [J]. J. Biol. Chem., 1982, 257, 7872— 7878.
[88] Roth KA, Hertz JM, Gordon JI. Mapping enter endocrine cell populations in transgenic mice reveals an unexpected degree of complexity in cellular differentiation within the gastrointestinal tract [J]. J cell Bile, 1990,100,1791—1801
[89] Clarke SD, Armstrong MK. Cellular lipid binding proteins: expression, function, and nutritional regulation [J]. FASEB J, 1989, 3(13): 2480—2487
[90] 林万华,黄路生,任军,等. 中外十个猪种H-FABP基因遗传变异的研究[J]. 遗传学报, 2002, 29(1): 12-15
[91] Distel R. J. et al. Fatty acid regulation of gene expression. [J]. J Bio Chem. 1992, 267, 5937—5941.
[92] Santiago M. Di Pietro, Jose' A. Santome'. Structural and Biochemical Characterization of the Lungfish (Lepidosiren paradoxa) Liver Basic Fatty Acid Binding Proteinl [J]. Archives of Biochemistry and Biophysics, 2001, 388, 81—90
[93] Mukesh K. Sharmaa, Eileen M. Denovan-Wrightb, Agnes Degravec, et al. Sequence, linkage mapping and early developmental expression of the intestinal-type fatty acid-binding protein gene (fabp2) from zebra fish[J]. Comparative Biochemistry and Physiology, 2004,Part B 138,391-395
[94] Thompson J D, Gibson T J, Plewniak F, et al. The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools [J]. Nucleic Acids Res, 1997, 24, 4876—4882.
[95] Saitou, Nei. The neighbor-joining method: A new method for reconstructing phylogenetic trees [J]. Molecular Biology and Evolution, 1987, 4, 406—425
[96] Felsenstein J. The troubled growth of statistical phylogenetics [J]. Syst Biol, 2001, 50(4) :465 -467.
[97] E.M. Denovan-Wright, M. Pierce, J.M. Wright, Nucleotide sequence of cDNA clones coding for a brain-type fatty acid binding protein and its tissue-specific expression in adult zebra fish (Danio rerio), Biochim. Biophys. Acta 2000, 1492, 223-228.
[98] S.M. Di Pietro, E.C. Dell'Angelica, C.H. Schleicher, J.A. Santome¨, Purification and structural characterization of a fatty acid-binding protein from the liver of the catfish Rhamdia sapo[J]. Comp. Biochem. Physiology. B Biochem. Mol. Biol. 1996,113 ,503-509.
[99] S.M. Di Pietro, E.C. Dell' Angelica, J.H. Veerkamp, N. Sterin-Spiziale, J.A. Santome, Amino acid sequence, binding properties and evolutionary relationships of the basic liver fatty-acid-binding protein from the catfish Rhamdia sapo[J]. Eur. J. Biochem. 1997, 249, 510-517.
[100] Sweetser DA, Hauft SM, Hoppe PC, Birkenmeier EH, Gordon JI. Transgenic mice containing intestinal fatty acid binding protein-human growth hormone fusion genes exhibit corrected regional and cell-specific expression of the reporter gene in their small intestine[J]. Proc. Natl. Acad. Sci. USA.1988, 85(24): 9611—9615
[101] Jones PD, Carne A, Bass NM, Grigor MR. Isolation and characterization of fatty acid binding proteins from mammary tissue of lactating rats[J]. Biochemistry. J.1988, 251(3): 919- 925
[102] Bass, N. M. et al, Tissue expression of three structurally different fatty acid binding proteins from rat heart muscle, liver, and intestine[J]. Biochem. Biol.Res. Commun. 1986, 137, 929-935
[103] Bass NM, Manning JA, Ockner RK, Gordon JI, Seetharam S, Alpers DH. Regulation of the biosynthesis of two distinct fatty acid-binding proteins in rat liver and intestine. Influences of sex difference and of clofibrate[J]. J. Biol.Chem. 1985a, 260(3): 1432—1436
[104] Paulussen RJ, van Moerkerk HT, Veerkamp JH. Immunochemical quantitation of fatty acid-binding proteins. Tissue distribution of liver and heart FABP types in human and porcine tissues[J]. Int. J. Biochem. 1990, 22(4): 393—398
[105] Bass NM, Manning JA, Ockner RK. Turnover and short-term regulation of fatty acid binding protein in fiver[J]. J. Biol.Chem. 1985b, 260(17): 9603—9607
[106] Sheridan M, Wilkinson TC, Wilton DC. Studies on fatty acid-binding proteins. Changes in the concentration of hepatic fatty acid-binding protein during development in the rat[J]. Biochem. J.1987, 42(3): 919—922
[107] Levin MS, Pitt AJ, Schwartz AL, Edwards PA, Gordon JI. Developmental changes in the expression of genes involved in cholesterol biosynthesis and lipid transport in human and rat fetal and neonatal livers[J]. Biochem. Biop s. Acta .1989, 1003(3): 377-382.
[108] Norbert H, Haunerland, et al; Fatty acid-binding proteins-insights from genetic manipulations[J]. Progress in Lipid Research, 2004, 43: 328—349
[109]Marti A,et al,Down-regulation of heart HFABP and UCP2 gene expression in diet-induced(cafeteria) obese rats[J].J.Physiol.Biochem.2002,58(2):69-74
[110]Kang K,et al,CLA inhibits differentiation of adiposities and decreases PPARgamma expression[J].Biochem.Biophys.Res.Commun.2003,303(3):795-799
[111]Londraville RL,Duvall CS.Murine leptin injections increase intracellular fatty acid-binding protein in green sunfish(Lepomis cyanellus)[J].Gen.Comp.Endocrinol.2002,129(1):56-62
[112]Piro S,Anello M,Di Pietro C,Lizzio MN,Patane G,Rabuazzo AM,Vigneri R,Purrello M,Purrello F.Chronic exposure to free fatty acids or high glucose induces apoptosis in rat pancreatic islets:possible role of oxidative stress[J].Metabolism.2002,51(10):1340-1347
[113]Glatz JF,et al,Diurnal variation of cytosofic fatty acid binding protein content and of palinitate oxidation in rat fiver and heart[J].J.Biol.Chem.1984,259(7):4295-4300
[114]Kontani Y,et al,Effects of aging and denervation on the expression of uncoupling proteins in slow and fast-twitch muscles of rats[J].J.Biochemistry.2002,132(2):309-315
[115]Clavel S,Farout L,Briand M,Briand Y,Jouanel P.Effect of endurance training and/or fish oil supplemented diet on cytoplasmic fatty acid binding protein in rat skeletal muscles and heart[J].Euro.J.Appl.Physiology.2002,87(3):193-201
[116]Veerkamp JH,van Moerkerk HT.Fatty acid binding protein and its relation to fatty acid oxidation[J].Mol.Cell Biochem.1993,123(2):101-106
[117]Maeda K,Uysal KT,Makowski L Gorgun CZ,Atsumi G,Parker RA,Bruning J,Hertzel AV,Bernlohr DA,Hotamisligil GS.Role of the fatty acid binding protein mall in obesity and insulin resistance[J].Diabetes;2003,52(2):300-307
[118]韩为东,于力.RACE技术在钓取白血病相关基因LRP16全长cDNA中的应用[J].中国实验血液科学杂志,2001,9(1):18-21
[119]王锋超,高京生.由EST到全长cDNA-RACE及相关技术进展[J].生命的化学,2002,22(3):294-297
[120]张庆华,茅矛.基因组研究中全长cDNA克隆的策略[J].生物工程进展,2000,20(4):3-5.
[121]杜占文,刘立仁.用RACE结合cDNA文库筛选的方法获取新的锌指蛋白基因[J].遗传,2002,24(3):329-331.
[122]邱为民,张思仲.一种新的cDNA末端快速扩增获取全长cDNA的方法[J].遗传,2001,23(5):480-482.
[123]萨姆布鲁克,弗里奇,曼里阿蒂斯.分子克隆实验指南(第二版)[M]。金冬雁,黎阵枫,侯云德.译.北京:科学出版社,1998,16-33.
[124]ZHANG Yong,ZHANG Wei-Min,LI Xin,et al.Construction of cDNA Library from the Ovary of Epinephelus coiodes and Cloning of a Fatty Acid Binding Protein(FABP)[J].Acta Scientiarun Naturalium Univerisitatis Sunyatseni,2003,42(2):66-69.
[125]Santiago M Di Pietro,Jose A Santome.Structural and Biochemical Characterization of the Lungfish(Lepidosiren paradoxa) Liver Basic Fatty Acid Binding Protein[J].Archives of Biochemistry and Biophysics,2001,388,81-90.
[126]黄玉海,汪以真,单体中,等.猪心型脂肪酸结合蛋白基因的克隆及不同生长阶段表达的差异[J].农业生物技术学报,2006,14(5):657-661.
[127]Mukesh K Sharmaa,Eileen M Denovan-Wrightb,Agnes Degravec,et al.Sequence,l-inkage mapping and early developmental expression of the intestinal-type fatty acid-binding protein gene(fabp2) from zebra fish[J].Comparative Biochemistry and Physiology:Part B,2004,138,391-399.
[128]敖金霞.鹅GH和A-FABP基因的克隆及基因多态性与鸡生长和体组成性状的相关研究[D].哈尔滨:东北农业大学,2003.
[129]黄河,帅素容,余先琼,等.藏猪心脏脂肪酸结合蛋白基因(H-FABP)组织表达差异性研究[J].四川农业大学学报,2007,25(4):480-483.
[130]林万华,黄路生,任军,等.H-FABP基因型对二花脸猪相关性状影响的初步分析[J],畜牧兽医学报,2003,34(4):318-324.
[131]李惠侠,杨公社,卢建雄.花生四烯酸对大鼠前体脂肪细胞生长与分化的影响[J].中国生物化学与分子生物学报,2005,06,27-31.
[132]Gerben F D,Koning D J,Harders F L,et al.Effect of genetic variants of the hear-t fatty acid binding protein gene on intransmuscular fat and performance traits in pig[J].Journal of Animal Science,1999,77:846-852.
[133]孟和,王桂华,王启贵,等.鸡PPAR基因单核苷酸多态与脂肪性状的相关研究[J].遗传学报,2002,29(2):119-123.
[134]Rong-Zong Liu,Eileen M Denovan-Wright Jonathan M Wright.Structure,mRNA ex-pression and linkage mapping of the brain-type fatty acid-binding protein gene(fabp7) from zebra fish(Danio rerio)[J].Journal of Europe Biochemical.,2003,270:715-725.
[135]Guor Mour Her,Yang-Hui Yeh,Jen-Leih Wu.Functional Conserved Elements Media-te Intestinal-Type Fatty Acid Binding Protein(I-FABP) Expression in the Gut Epithelia of Zebra fish Larvae[J].Developmental Dynamics,2004,230:734-742.
[136]Rong-Zong Liu,Eileen M Denovan-Wright,Jonathan M Wright.Structure,linkage mapping and expression of the heart-type fatty acid-binding protein gene(fabp3) from zebra fish(Danio rerio)[J].Journal of Europe Biochemical,2003,270,3223-3234.
[137]Eileen M Denovan Wright,Marianne Pierce,Mukesh K Sharma,et al.cDNA sequence and tissue-specific expression of a basic liver-type fatty acid binding protein adult zebra fish (Danio rerio)[J].Biochemical & Biophysical Acta,2000,1492,227-232.