西芹根物质与挥发物对黄瓜枯萎病菌的化感作用、机理及化感物质分离纯化的研究
|
摘要
黄瓜枯萎病是世界性根际土传病害,发病率高,严重威胁和阻碍黄瓜生产。而利用化感作用防治病害是当今植物保护的新方法。
本试验利用生长速率法、柱层析法等方法,研究了西芹根物质与挥发物对黄瓜枯萎病菌的化感作用,并深入系统地研究了其对黄瓜枯萎病菌的化感作用机理,且对化感物质进行了分离纯化,并进行GC/MS分析,确定化感物质成分,为利用化感作用防治黄瓜枯萎病及开发植物源抑菌剂提供了理论依据,主要结果如下：
1.西芹鲜根及根际区物各浸提液对黄瓜枯萎病菌的生长具有化感作用,在一定浓度范围内对黄瓜枯萎病菌有明显的化感抑制作用,浓度越高,抑制作用越强,其中西芹鲜根及根际区物丙酮浸提液最高抑制效果分别为33.97%、39.26%,乙醇浸提液最高抑制效果分别为42.31%、94.37%,蒸馏水浸提液最高抑制效果分别为38.07%、21.27%。乙醇浸提液对供试病原菌的化感抑制效果最好。西芹鲜根及根际区物各浸液对黄瓜枯萎病菌的化感作用的强弱与浸提液的浓度和作用时间有关。
2.黄瓜枯萎病菌经西芹鲜根及根际区物浸提液处理后,其菌体内保护酶(SOD、CAT)活性均显著低于对照,分泌的细胞壁降解酶(纤维素酶、果胶酶、β-葡萄糖苷酶)活性均低于对照,菌体内MDA、可溶性蛋白质含量较对照明显升高。
3.西芹鲜根及根际区物各浸提液处理后黄瓜植株活性氧代谢指标(SOD、 POD、CAT).防御酶体系指标(PPO、PAL、几丁质酶活性)均高于对照,细胞膜透性指标(MDA、可溶性蛋白质、H2O2含量)均低于对照。
4.对西芹鲜根及根际区物不同浸提液二次层析后化感效应最佳流分进行分离鉴定,得出：西芹鲜根与根际区物丙酮浸提液二次层析后化感效应最佳流分的主要化感成分为2-丙烯酸十五烷基酯；西芹鲜根与根际区物乙醇浸液二次层析后化感效应最佳流分的主要化感成分均为4-二甲氨基-2-甲基-1-苯基-丁-2-醇、8-十八烯酸甲酯、棕榈酸甲酯、十八烷二烯酸甲酯；西芹鲜根与根际区物蒸馏水浸液二次层析后化感效应最佳流分的主要化感成分为十六烷基二甲基叔胺、14-甲基十五烷酸甲酯、六甲基环三硅氧烷、(E)-9-十八烯酸甲酯、蓖麻油酸甲酯、16-甲基十七烷酸甲酯。
5.西芹挥发物对黄瓜枯萎病菌具有化感作用,且这种作用随着挥发物浓度增加而增强,同时随着时间延长而减弱。各离体材料(西芹种子、叶片、叶柄、鲜根)均表现为重量为200g的处理抑制作用最强,至处理144h后,其化感效果分别为9.27%、10.93%、12.24%、18.74%；西芹鲜根挥发物的化感作用最强,其次为叶柄,再次为叶片,种子最弱。对共培养后接种病菌的黄瓜幼苗发病情况的调查统计表明,西芹挥发物抑制发病的作用随着浓度的增加而增强,时间增加,效果减弱。在整个测定时期,各处理SOD活性、POD活性、CAT活性、几丁质酶活性、PPO活性、PAL活性均较对照升高,并且均呈现先升高后降低的变化趋势,MDA含量均低于对照,且表现为先降低后升高的变化趋势；各处理SOD活性、POD活性、CAT活性、几丁质酶活性、PPO活性、PAL活性均随着活体西芹挥发物浓度的增加而增加,MDA含量随着活体西芹挥发物浓度的增加而减少。
6.本文较全面地对西芹根物质与挥发物对黄瓜枯萎病菌的化感作用、机理及化感物质分离、纯化、鉴定进行了研究,创新点如下：
①研究了西芹根物质浸提液对黄瓜枯萎病菌的化感作用效果,为蔬菜病害生物防治和可持续发展奠定理论基础。
②从黄瓜枯萎病菌和黄瓜植株两个角度研究了化感作用机理,使研究角度更加全面。特别是利用液体培养基研究了黄瓜枯萎病菌菌体本身代谢酶活性及物质含量变化,这种研究方式所见不多。
③分离、纯化、鉴定了西芹鲜根及根际区物浸提液的化感物质成分,为化感物质在生产实践中的应用提供了科学依据。
④研究了西芹挥发物对黄瓜枯萎病菌的化感作用,探索化感作用研究的新领域,为多方面综合防治蔬菜病害提供了新思路。
Fusarium disease is a worldwide rhizosphere soil-borne disease, the disease incidence increased significantly, which threaten and impeded seriously the production of cucumber. Using allelopathy to prevent soil-borne diseases is a method in today's plant protection.
The growth rate method and the column chromatography were used to study the allelopathy of the root、 the rhizosperic soil and the volatile of parsley on Fusarium oxysporium f.sp.cucumeris, and further research the allelopathy mechanism on Fusarium oxysporium f.sp.cucumeris, and preliminary allelochemicals separation with the strongest allelopathic extracts, and then the allelochemicals were analysed by the GC/MS to determine the composition which provided scientic and theoretical foundation for preventing fusarium disease by using allelopathy and developing antibacterial agent of plant source. The main results were as follows:-
1. The extracts of the root and the rhizosperic soil of parsley had allelopathic effect on Fusarium oxysporium f.sp.cucumeris, and which had obviously inhibition in certain range of concentration, The higher the concentration, the stronger the effect of allelopathy. The highest inhibition rates of the acetone extracts of the root and the rhizosperic soil of parsley were33.97%and39.26%, the highest inhibition rates of the ethanol extracts were42.31%and94.37%, the highest inhibition rates of the aqueous extracts were38.07%and21.27%. The ethanol extracts showed the strongest inhibitory effeet on Fusarium oxysporium f.sp.cucumeris.
2. After treat with the extracts of the root and the rhizosperic soil of parsley, the intracellular protective enzyme (SOD, CAT) activity decreased compared with the control, and the cell wall-degrading enzymes (cellulase,pectinase,β-glucosidase) was lower than the control, and the MDA content and soluble protein content increased compared with the control.
3. The intracellular protective enzyme (SOD, POD, CAT), the PPO, PAL and chitinase activity of the cucumber seedlings were higher than the control after treat with the extracts of the root and the rhizosperic soil of parsley, and the MDA content, soluble protein and H2O2content were lower than the control.
4. The main allelochemicals of the best ablelopathy fraction of the acetone extract in the root and the rhizosperic soil of parsley after secondary separation were2-Propenoic acid, pentadecyl ester; the main allelochemicals of the best allelopathy fraction of the ethanol extract in the root and the rhizosperic soil of parsley after secondary separation were4-Dimethylamino-2-methyl-l-phenyl-butan-2-ol8-Octadecenoic acid, methyl ester、Hexadecanoic acid, methyl ester、Octadecadienoic acid, methyl ester; the main allelochemicals of the best allelopathy fraction of the aqueous extract in the root and the rhizosperic soil of parsley after secondary separation were1-Hexadecanamine, N,N-dimethyl-、 Pentadecanoic acid,14-methyl-, methyl ester、 Cyclotrisiloxane, hexamethyl-、9-Octadecenoic acid, methyl ester,(E)-Methyl ricinoleate、Heptadecanoic acid,16-methyl-, methyl ester.
5. The parsley volatile did have allelopathic effect on Fusarium oxysporium f.sp. cucumeris, and the effect increased with increasing concentration and dropped down with extending time. The allelopathy effect respectively were9.27%,10.93%,12.24%,18.74%after treated with the volatile of the seed, leaf, petiole, root of parsley of200g for144h. The parsley root volatile showed the strongest allelopathy, followed by the petiole, and third the leaf, and finally the seed. The incidence of cucumber was studied after inoculated bacteria, and the results showed that the allelopathy effect increased with increasing concentration and dropped down with extending time. The SOD activity, POD activity, CAT activity, Chitinase activity, PPO activity, PAL activity were higher than that of the contrast at the different time, and increased at first and then decreased; the content of MDA was lower than that of the contrast during the measurement period, and decreased at first and then increased. The SOD activity, POD activity, CAT activity, Chitinase activity, PPO activity, PAL activity increased with increasing concentration; the content of MDA dropped down with increasing concentration.
6. The allelopathy, mechanism and allelochemicals identification of the root material and volatile of parsley on Fusarium oxysporium f.sp.cucumeris were studied comprehensively. The innovative points were as follows:
①The allelopathy effects of the extrects of the root material of parsley on Fusarium oxysporium f.sp.cucumeris were studied to provide theoretical foundation for biological control of vegetable diseases and sustainable development.
②The mechanism was studied from two perspectives of Fusarium oxysporium f.sp.cucumeris and cucumber seedlings, which made the research angle more comorehensively. Especially the liquid medium was used to study the changes of enzymes activity and material contents metabolized out from Fusarium oxysporium f.sp.cucumeris, which had not been widely applied.
③The allelochemicals in the root and the rhizosperic soil of parsley were isolated, purificated and identificated to provide scientic basis for applicating allelochemicals in production practice.
④The allelopathy of parsley volatile on Fusarium oxysporium f.sp.cucumeris was studied to explore the new field in the study of allelopathy, which provided a new train of thought for comprehensive prevention and treatment of vegetable diseases in many ways.
本试验利用生长速率法、柱层析法等方法,研究了西芹根物质与挥发物对黄瓜枯萎病菌的化感作用,并深入系统地研究了其对黄瓜枯萎病菌的化感作用机理,且对化感物质进行了分离纯化,并进行GC/MS分析,确定化感物质成分,为利用化感作用防治黄瓜枯萎病及开发植物源抑菌剂提供了理论依据,主要结果如下：
1.西芹鲜根及根际区物各浸提液对黄瓜枯萎病菌的生长具有化感作用,在一定浓度范围内对黄瓜枯萎病菌有明显的化感抑制作用,浓度越高,抑制作用越强,其中西芹鲜根及根际区物丙酮浸提液最高抑制效果分别为33.97%、39.26%,乙醇浸提液最高抑制效果分别为42.31%、94.37%,蒸馏水浸提液最高抑制效果分别为38.07%、21.27%。乙醇浸提液对供试病原菌的化感抑制效果最好。西芹鲜根及根际区物各浸液对黄瓜枯萎病菌的化感作用的强弱与浸提液的浓度和作用时间有关。
2.黄瓜枯萎病菌经西芹鲜根及根际区物浸提液处理后,其菌体内保护酶(SOD、CAT)活性均显著低于对照,分泌的细胞壁降解酶(纤维素酶、果胶酶、β-葡萄糖苷酶)活性均低于对照,菌体内MDA、可溶性蛋白质含量较对照明显升高。
3.西芹鲜根及根际区物各浸提液处理后黄瓜植株活性氧代谢指标(SOD、 POD、CAT).防御酶体系指标(PPO、PAL、几丁质酶活性)均高于对照,细胞膜透性指标(MDA、可溶性蛋白质、H2O2含量)均低于对照。
4.对西芹鲜根及根际区物不同浸提液二次层析后化感效应最佳流分进行分离鉴定,得出：西芹鲜根与根际区物丙酮浸提液二次层析后化感效应最佳流分的主要化感成分为2-丙烯酸十五烷基酯；西芹鲜根与根际区物乙醇浸液二次层析后化感效应最佳流分的主要化感成分均为4-二甲氨基-2-甲基-1-苯基-丁-2-醇、8-十八烯酸甲酯、棕榈酸甲酯、十八烷二烯酸甲酯；西芹鲜根与根际区物蒸馏水浸液二次层析后化感效应最佳流分的主要化感成分为十六烷基二甲基叔胺、14-甲基十五烷酸甲酯、六甲基环三硅氧烷、(E)-9-十八烯酸甲酯、蓖麻油酸甲酯、16-甲基十七烷酸甲酯。
5.西芹挥发物对黄瓜枯萎病菌具有化感作用,且这种作用随着挥发物浓度增加而增强,同时随着时间延长而减弱。各离体材料(西芹种子、叶片、叶柄、鲜根)均表现为重量为200g的处理抑制作用最强,至处理144h后,其化感效果分别为9.27%、10.93%、12.24%、18.74%；西芹鲜根挥发物的化感作用最强,其次为叶柄,再次为叶片,种子最弱。对共培养后接种病菌的黄瓜幼苗发病情况的调查统计表明,西芹挥发物抑制发病的作用随着浓度的增加而增强,时间增加,效果减弱。在整个测定时期,各处理SOD活性、POD活性、CAT活性、几丁质酶活性、PPO活性、PAL活性均较对照升高,并且均呈现先升高后降低的变化趋势,MDA含量均低于对照,且表现为先降低后升高的变化趋势；各处理SOD活性、POD活性、CAT活性、几丁质酶活性、PPO活性、PAL活性均随着活体西芹挥发物浓度的增加而增加,MDA含量随着活体西芹挥发物浓度的增加而减少。
6.本文较全面地对西芹根物质与挥发物对黄瓜枯萎病菌的化感作用、机理及化感物质分离、纯化、鉴定进行了研究,创新点如下：
①研究了西芹根物质浸提液对黄瓜枯萎病菌的化感作用效果,为蔬菜病害生物防治和可持续发展奠定理论基础。
②从黄瓜枯萎病菌和黄瓜植株两个角度研究了化感作用机理,使研究角度更加全面。特别是利用液体培养基研究了黄瓜枯萎病菌菌体本身代谢酶活性及物质含量变化,这种研究方式所见不多。
③分离、纯化、鉴定了西芹鲜根及根际区物浸提液的化感物质成分,为化感物质在生产实践中的应用提供了科学依据。
④研究了西芹挥发物对黄瓜枯萎病菌的化感作用,探索化感作用研究的新领域,为多方面综合防治蔬菜病害提供了新思路。
Fusarium disease is a worldwide rhizosphere soil-borne disease, the disease incidence increased significantly, which threaten and impeded seriously the production of cucumber. Using allelopathy to prevent soil-borne diseases is a method in today's plant protection.
The growth rate method and the column chromatography were used to study the allelopathy of the root、 the rhizosperic soil and the volatile of parsley on Fusarium oxysporium f.sp.cucumeris, and further research the allelopathy mechanism on Fusarium oxysporium f.sp.cucumeris, and preliminary allelochemicals separation with the strongest allelopathic extracts, and then the allelochemicals were analysed by the GC/MS to determine the composition which provided scientic and theoretical foundation for preventing fusarium disease by using allelopathy and developing antibacterial agent of plant source. The main results were as follows:-
1. The extracts of the root and the rhizosperic soil of parsley had allelopathic effect on Fusarium oxysporium f.sp.cucumeris, and which had obviously inhibition in certain range of concentration, The higher the concentration, the stronger the effect of allelopathy. The highest inhibition rates of the acetone extracts of the root and the rhizosperic soil of parsley were33.97%and39.26%, the highest inhibition rates of the ethanol extracts were42.31%and94.37%, the highest inhibition rates of the aqueous extracts were38.07%and21.27%. The ethanol extracts showed the strongest inhibitory effeet on Fusarium oxysporium f.sp.cucumeris.
2. After treat with the extracts of the root and the rhizosperic soil of parsley, the intracellular protective enzyme (SOD, CAT) activity decreased compared with the control, and the cell wall-degrading enzymes (cellulase,pectinase,β-glucosidase) was lower than the control, and the MDA content and soluble protein content increased compared with the control.
3. The intracellular protective enzyme (SOD, POD, CAT), the PPO, PAL and chitinase activity of the cucumber seedlings were higher than the control after treat with the extracts of the root and the rhizosperic soil of parsley, and the MDA content, soluble protein and H2O2content were lower than the control.
4. The main allelochemicals of the best ablelopathy fraction of the acetone extract in the root and the rhizosperic soil of parsley after secondary separation were2-Propenoic acid, pentadecyl ester; the main allelochemicals of the best allelopathy fraction of the ethanol extract in the root and the rhizosperic soil of parsley after secondary separation were4-Dimethylamino-2-methyl-l-phenyl-butan-2-ol8-Octadecenoic acid, methyl ester、Hexadecanoic acid, methyl ester、Octadecadienoic acid, methyl ester; the main allelochemicals of the best allelopathy fraction of the aqueous extract in the root and the rhizosperic soil of parsley after secondary separation were1-Hexadecanamine, N,N-dimethyl-、 Pentadecanoic acid,14-methyl-, methyl ester、 Cyclotrisiloxane, hexamethyl-、9-Octadecenoic acid, methyl ester,(E)-Methyl ricinoleate、Heptadecanoic acid,16-methyl-, methyl ester.
5. The parsley volatile did have allelopathic effect on Fusarium oxysporium f.sp. cucumeris, and the effect increased with increasing concentration and dropped down with extending time. The allelopathy effect respectively were9.27%,10.93%,12.24%,18.74%after treated with the volatile of the seed, leaf, petiole, root of parsley of200g for144h. The parsley root volatile showed the strongest allelopathy, followed by the petiole, and third the leaf, and finally the seed. The incidence of cucumber was studied after inoculated bacteria, and the results showed that the allelopathy effect increased with increasing concentration and dropped down with extending time. The SOD activity, POD activity, CAT activity, Chitinase activity, PPO activity, PAL activity were higher than that of the contrast at the different time, and increased at first and then decreased; the content of MDA was lower than that of the contrast during the measurement period, and decreased at first and then increased. The SOD activity, POD activity, CAT activity, Chitinase activity, PPO activity, PAL activity increased with increasing concentration; the content of MDA dropped down with increasing concentration.
6. The allelopathy, mechanism and allelochemicals identification of the root material and volatile of parsley on Fusarium oxysporium f.sp.cucumeris were studied comprehensively. The innovative points were as follows:
①The allelopathy effects of the extrects of the root material of parsley on Fusarium oxysporium f.sp.cucumeris were studied to provide theoretical foundation for biological control of vegetable diseases and sustainable development.
②The mechanism was studied from two perspectives of Fusarium oxysporium f.sp.cucumeris and cucumber seedlings, which made the research angle more comorehensively. Especially the liquid medium was used to study the changes of enzymes activity and material contents metabolized out from Fusarium oxysporium f.sp.cucumeris, which had not been widely applied.
③The allelochemicals in the root and the rhizosperic soil of parsley were isolated, purificated and identificated to provide scientic basis for applicating allelochemicals in production practice.
④The allelopathy of parsley volatile on Fusarium oxysporium f.sp.cucumeris was studied to explore the new field in the study of allelopathy, which provided a new train of thought for comprehensive prevention and treatment of vegetable diseases in many ways.
引文
1 Rice E L. Allelopathy(2rk edition). Academic Press.1984,1-50
2 W ittackerR H, Feeny P P. Allelochemics:Chemical interactions between species [J]. Science,1971,171(3937):757-770
3孔垂华,徐涛,胡飞.胜红蓟化感作用研究Ⅱ.主要化感物质的释放途径和活性[J].应用生态学报,1998,9(3)：257-260
4 张艳杰,鲁顺保,高捍东.南方红豆杉种子不同溶剂提取液对白菜种子萌发及幼苗生长的化感效应[J].中国农学通报,2010,26(9)：190-194
5曲波,王承旭,陈旭辉,等.刺萼龙葵种子提取物对白菜种子及幼苗化感作用的研究[J].第三届全国生物入侵大会论文摘要集
6 肖春萍,刘博,杨莉,等.加拿大蓬种子生态适应性及其化感作用[J].吉林农业大学学报,2011,33(5)：5279-531
7 郭晓霞,沈益新,李志华.几种豆科牧草地上部水浸提液对稗草种子和幼苗的化感效应[J].草地学报,2006,14(4)：356-359
8 陈礼玲,庞珂佳,李同臣,等.角茴香根和地上部水浸液对杂草反枝苋的化感作用[J].西北林学院学报,2011,26(1)：138-142
9 刘建新,胡浩斌,王鑫.黄瓜地上部水浸液对番茄的化感抑制效应[J].中国生态农业学报,2009,17(2)：312-317
10张凤丽,周宝利,何雨.嫁接茄子地上部浸提液的化感作用研究[J].安徽农业科技,2010,38(5)：2302-2303
11彭晓邦,程飞,张硕新.核桃叶水浸液对不同产地黄芩的化感效应[J].草地学报,2011,19(5)：839-845
12韩志军,陈静,郑寒,等.花椒叶浸提液对大豆种子萌发和幼苗生长的化感作用[J].应用与环境生物学报,2011,17(4)：585-588
13孙志蓉,翟明普,贺润平,等.甘草柠条水浸物对种子萌发和幼苗生长的相互影响[J].中国中药杂志,2008,33(21)：2459-2463
14张汝民,王玉芝,侯平,等.几种牧草幼苗对冷蒿茎叶水浸提液化感作用的生理响应[J].生态学报,2010,30(8)：2197-2204
15李发林,黄炎和,张汉荣,等.几种水土保持型牧草叶浸提液化感作用效应[J].中国农学通报,2010,26(18)：401-405
16李美娜,李志华.紫花苜蓿茎叶浸提液对波斯婆婆纳化感效应的研究[J].中国草地学报,2010,32(1)：68-74
17王辉,谢永生,杨亚利,等.云雾山铁杆蒿茎叶浸提液对封育草地四种优势植物的化感效应[J].生态学报,2011,31(20)：6013-6021
18张凤娟,徐兴友,陈凤敏,等.黄顶菊茎叶浸提液对白菜和水稻幼苗化感作用的初步研究[J].西北植物学报,2008,28(8)：1669-1674
19陈业兵,王金信,彭学岗,等.银胶菊叶对茼麻和稗的化感作用[J].植物保护学报,2009,36(1)：77-81
20张敏,付冬梅,陈华保,等.紫茎泽兰叶片对小麦、油菜幼苗的化感作用及化感机制的初步研究[J].浙江大学学报(农业与生命科学版)2010,36(5)：547-553
21李志华,沈益新.红三叶茎叶对几种牧草种子及幼苗的化感作用[J].中国草地,2005,27(3)：41-43
22曹兵,宋丽华,张涛.臭椿根浸提液对刺槐幼苗生长的化感效应[J].西北农业学报,2009,18(3)：156-159
23李坤,郭修武,郭印山,等.葡萄根系浸提液的化感作用[J].应用生态学报,2010,21(7)：1779-1784
24李志华,沈益新.不同品种紫花苜蓿根水浸提液化感作用效应的研究[J].中国草地,2005,27(4)：39-46
25 Rice E L, Putnam A R,Tang C S. The Science of Allelopathy[M]. New York, John Wileye Sons,1986
26周凯,郭维明.加拿大一枝黄花根系和根际土壤水浸液对萝卜和白菜种子萌发及幼苗生长的影响[J].西北植物学报,2005,25(1)：174-178
27郭兰萍,黄璐琦,蒋有绪,等.不同化学型苍术根茎及根际土提取物生物活性及化感物质的比较[J].中国药学杂志,2006,41(10)：734-737
28郭兰萍,黄璐琦,蒋有绪,等.苍术根茎及根际土水提物生物活性研究及化感物质的鉴定[J].生态学报,2006,26(2)：528-535
29梁文举,张晓珂,姜勇等.根分泌的化感物质及其对土壤生物产生的影响[J].地球科学进展,2005,20(3).330-335
30 Rovira.A. D. et al.Plant root exudates[J]. Bot Rev.,1969,35-57
31 Vancura, V. Root exudates of plants[J].Plant and soil,1964,21:231-248
32切尔诺布里维卡,C.H.植物分泌物的生物学作用和间作中的种间相互关系[M].北京：科学出版社,1961
33张丽丽,王颖,王辉,等.侧柏根际土壤提取物对4种植物种子萌发和幼苗生长的化感效应[J].河北农业大学学报,2008,31(6)：83-86
34曹兵,宋丽华,张婷婷.臭椿根际土壤化感作用的初步研究[J].西北林学院学报,2009,24(4)：147-150
35董淑琦,马永清,税军峰,等.不同年代冬小麦品种根际土浸提液诱导小列当种子发芽的化感作用研究[J].中国农业大学学报,2009,14(2)：59-63
36张顺捷,马凤鸣,王玉波,等.大豆根系分泌物化感作用的初步研究[J].东北农业大学学报,2008,39(10)：1-5
37张凤丽,周宝利,王茹华,等.嫁接茄子根系分泌物的化感效应[J].应用生态学报,2005,16(4)：750-753
38董小艳,程智慧,张亮.百合根系分泌物对4种观赏植物的化感作用[J].西北农林科技大学学报(自然科学版),2008,36(9)：113-117
39周艳丽,程智慧,孟焕文.大蒜根系分泌物对不同受体蔬菜的化感作用[J].应用生态学报,2007,18(1)：81-86
40由海霞,梁银丽,吕文,等.不同作物根系分泌物对黄瓜的化感作用[J].西北农林科技大学学报(自然科学版),2006,34(6)：101-105
41陈淑芳,曹晓华.南瓜根系及根部土壤水浸液化感作用研究[J].热带作物学报,2011,32(12)：2268-2273
42 Molisch H. Der Einfluss einer Pflanze auf die andere -Allelopathie. Fischer, Jena.1937
43李绍文.生态生物化学[M].北京：北京大学出版社,2001
44 Muller C.H.,Muller W. H. and Haines B.L. Volatile growth inhibitors production by shrubs[J]. Science.1964,143:471-473
45江贵波,陈少雄,黄丹莹,等.夹竹桃挥发物的化感作用研究[J].江西农业学报,2008,20(7)：62-64
46刘小香,王真辉,陈秋波.巨尾桉挥发物化感作用初步研究[J].热带作物学报,2008,29(3)：338-341
47江贵波,曾任森.艾的挥发性物质化感作用研究[J].生态科学,2006,25(2)：106-108
48张晓芳,王金信,谢娜,等.白三叶草挥发物的化感作用及其化学成分分析[J].植物保护学报,2011,38(4)：374-378
49曹子林,王晓丽,杨桂荚.紫茎泽兰气态挥发物对云南松种子萌发及幼苗生长的化感作用[J].江西农业大学学报,2012,34(1)：77-81
50孔垂华,徐涛,胡飞.胜红蓟化感作用研究Ⅱ.主要化感物质的释放途径和活性[J],应用生态报,1998,9(3)：257-260
51黎华寿,黄京华,张修玉,等.香茅天然挥发物的化感作用及其化学成分分析[J].应用生态学报,2005,6(4)：763-767
52马瑞君,王明理,朱学泰,等.黄帚橐吾挥发物的化感作用及其主要成分分析[J].应用生态学报,2005,16(10)：1826-1829
53周志红,骆世明,牟子平.番茄(lycopersicon esculentum)的化感作用研究[J].应用生态学报,1997,8(4)：445-449
54程智慧,金瑞,佟飞.蒜苗挥发物对黄瓜幼苗生长的化感效应研究[J].西北农业学报,2007,16(3)：149-152
55马卫宾,颜霞,谢慧丽.小麦化感作用对非根际土壤微生物区系的影响初探[J].陕西农业科学,2007(4)：35-37
56沈荔花,郭琼霞,林文雄,等.加拿大一枝黄花对土壤微生物区系的影响研究[J].中国农学通报,2007,23(4)：323-327
57 Sturz A V, Christie B R, Matheson B G. Association of bacterial endophyte populations from red clover and potato crops with popential for beneficial allelopathy [J]. Can-J-Microbiol,1998,44(2):162-167
58 FrankA E, Gerald R L. Potentials forexploiting allelopathy to enhance crop product ion[J]. JChem Ecol,1988,14 (10):1829-1844
59李天昊.膜荚黄芪地上部水浸液对植物、昆虫及真菌的影响研究[D].吉林农业大学,2007
60朱文杰.入侵植物加拿大一枝黄花对土传病菌的化感作用[D].浙江大学,2011
61周礼胜.芒萁化感作用及抑菌试验研究[D].南昌大学,2007
62周宝利,陈志霞,杜亮,等.不同抗病性茄子根系分泌物对黄萎病的化感作用[J].生态学报,2011,31(14)：3964-3972
63李云鹏,周宝利,李之璞,等.野生抗病茄植株腐解物对茄子黄萎病菌的化感作用研究[J].沈阳农业大学学报,2007,38(4)：500-503
64 Larkin R P. Effect of successive watermelon plantings on Fusarium oxysporum and other microorganisms in soils suppressive and conductive to Fusarium Wilt of water melon[J]. Phytopathology,1993,83:1097-1104
65 Yang C H, Crowley D, E and Menge J A.165r DNA fingerprinting of rhizosphere baeterial communities assoeiated with healthy and phyto phorainfected avocadotoots[J].FEMS Mierobiol Eeol,2000,35:129-136
66吴凤芝,潘凯,周秀艳.苯丙烯酸对黄瓜幼苗生理特性的影响[J].应用生态学报,2005,16(5)：915-918
67 Li F M,Hu M Y. Isolation and characterization of a novel antialgal allelochemical from phragmites communis[J]. Environmental Microbiology,2005,71 (11):6545-6553
68 Liu DL,Lovett JV. Biologically active secondary metabolites of barley. Ⅱ. phytotoxicity of barley allelochemicals[J]. J Chem Ecol,1993,19(10):2231-2244
69孔垂华,胡飞.植物化感相生相克作用及其应用[J].北京：中国农业出版社.2001
70 Bais.Harsh P.,Vepachedu. Ramarao, Gilroy. Simon, et al. Allelopathy and Exotic Plant Invasion: From Molecules and Genes to Species Interactions[J]. Sicience.2003, 301 (5638):1377-1380
71 Penuelas J,Ribas-Carbo M, Giles L. Effects of allelochemicals on plant respiration and oxygen isotope fractionation by the alternative oxidase[J].J Chem Ecol,1996,22 (4): 801-805
72 Hejl A M, Einhellig F A., Rasmussen J A. Effects of juglone on growth, phyotosynthesis,and respiration[J]. Journal of Chemical Ecology,1993,19 (3):931-939
73 Einhellig F A, Rasmussen J A,Hejl A M, et al. Effects of roots exudates sorgoleone on photosynthesis. [J]. Journal of Chemical Ecology,1993,19:369-372
74 David S S.Chemistry and mechanisms of Allelopathic interactions[J]. Agronomy Journal,1996, 88:876-885
75陈冬梅,林文雄.水稻化感作用研究现状与展望[J].福建农业大学学报,2000,(3)：281-285
76陈伟,叶明志,周洁.植物中酚类物质的变化[J].福建农业大学学报,1997,26(4)：502-508
77李绍文.生态生物化学(二)：高等植物之间的生化关系[J].生态学杂志,1989,8(1)：66-70
78孔垂华,黄寿山,胡飞.胜红蓟化感作用研究V.挥发油对真菌、昆虫和植物的生物活性及其化学成份[J].生态学报,2001,21(4)：584-587
79喻景权,松井佳久.豌豆根系分泌物自毒作用的研究[J].园艺学报,1999,26(3)：175-179
80孔垂华,胡飞.植物化感(相生相克)作用及其应用.中国农业出版社,北京.2001
81 Inderjit & Dakshini, K M M. On laboratory bioassays in allelopathy[J]. The botanical review.1995,61(1):28-44
82 Tang C H &Yong C C. Collection and identification of allelopathic compands from the undisturbed root system of Bigalta limpogress (Hemarthria altissima) [J]. Plant Physiol.,1982,69:155-160
83 Whitehead D C, et al. Bound phenolic compounds in wheat extracts of soil, plant roots and leaf litter[J]. Soil Biol. Biochem.,1983,15 (2):133-136
84 Thomas S C Wang, et al. Soil phenolic acids as plant inhibitors[J]. Soil Sci.,1967, 103(4):239-246
85 Vance G F, et al. Phenolic compounds in soils of hydrosequences and developmental sequences of spodosols [J]. Soil Sci. Soc. Am J.,1986,50:992-996
86 Kuwatsuka S&Shindo H. Behavior of phenolic substances in the decaying process of plants (I). Soil Sci. Plant Nutr.,1973,19:219-227
87 Yu J Q &Yoshihisa M. Extraction and identification of phytotoxic substances accumulated in nutrient solution for hydroponic culture of tomato [J].Soil Sci. Plant Nutr.,1993, 39(4):691-700
88 Vance G F,et al.Extraction of phenolic compounds from a spodosol profile:an evaluation of three extractants[J]. Soil Sci.,1985,140 (6):412-420
89阎飞,杨振明,韩丽梅.植物化感作用及其作用物的研究方法[J].生态学报,2000,20(4)：692-696
90曾任森,林象联,等.蟛蜞菊的生化他感作用及生化他感作用物的分离鉴定[J].生态学报,1996,16(1)：20-27
91 Kagan I A,Rimando A M,Dayan F E. Chromatographic separation and in vitro activity of sorgoleone congeners from the roots of Sorghum bicolor[J]. Journal Agricultural and Food Chemistry,2003,51(26):7589-7595
92孔垂华,徐效华.有机物的分离和结构鉴定[M].北京：化学工业出版社,2003
93 Bulen W A, et al. Sepration of organic acids from plant tissues chromatographic technique [J].Anal. Chem.,1952,24:187-190
94 Guenzi W D&McCalla. Phenolic acids in oats,whear, sorghum, and corn residues and their phytotoxicityAgro. J.,1966,58:303-304
95马瑞霞,等.小麦根区微生物分解小麦残体产生的化感物质及其生物活性的研究[J].生态学报,1996,16(6)：632-639
96 Grierson P F.Organic acids in the rhizosphere ofBanksia integrifoliaL. F[J].Plant and Soil,1992,144:259-265
97刘秀芬,等.根际他感化学物质的分离鉴定与生物活性的研究[J].生态学报.1996,16(1)：1-10
98 Iderjit &Dakshini K M M. allelopathic potential of an annual weed Polypogon monspeliensis in crops in India[J].Plant and Soil,1995,173:251-257
99王明道,陈红歌,刘新育,等.地黄对芝麻的化感作用及其化感物质的分离鉴定[J].植物生态学报,2009,33(6)：1191-1198
100张爱加,袁照年,陈冬梅,等.甘蔗根系土壤化感潜力评价及其化感物质分析[J].中国生态农业学报,2010,18(5)：1013-1017
101耿广东,张素勤,程智慧.甘辣椒根系分泌物的化感作用及其化感物质分析[J].园艺学报,2009,36(6)：873-878
148 西芹根物质与挥发物对黄瓜枯萎病菌的化感作用、机理及化感物质分离纯化的研究
102陈业兵,王金信,吴小虎,等.银胶菊的花对稗草的化感作用及其化感物质分离与鉴定[J].植物保护学报,2010,37(1)：73-77
103曾任森.化感作用研究中的生物测定方法综述[J].应用生态学报,1999,10(1)：123-126
104 Leather G R,Einhellig F A.Bioassay in the study of allelopathy [A]. In:Putnam A R and Tang C S(ed).The Science of Allelopathy[C]. New York: John Willy and Sons,1986,133-145
105 Mortley D G,Loretan P A, Hill W A, et al. Biocompatibility of sweetpotato and peanut in a hydroponicsystem. Hortscience.1998,33(7):1147-1149
106 Einhellig, F. A. Mechanisms and modes of action of allelochemicals[M]. In:The Science of Allelopathy. A. R.Putnam and C. S. Tang (ed). John Wiley&Sons. New York.1986, 171-188
107 Politycka B.Phenolics and the acivities of phenylalanine ammonia-lyase, phenol-beta-glucosyl transferase and beta-glucosidase in cucumber roots as affected by phenolic allelochemicals[J]. Acta Physiologiae Plantarum,1998,20(4):405-410
108 Zobel, A. M. and J. M. Lynch. Extrusion of UV—absorbing phenolics in Acer. In response to UV and freezing temperatulre I. U VA—absorbing compounds on the surface of Acer saccharum and Acer platanoides autum leaves [J].Allelopathy Journal,1997,4:269-276
109 Calera, M. R., Anaya,A. L. and R. M. Gavilanes. Effects of phytotoxic resin glycoside on activity of H+-ATPase from plasma membrane[J]. J. Chem. Ecol.,1995,21:289-297
110 Romagni, J. G. and Allen, N. and F. E. Dayan. Allelopathic effects of volatile cineoles two weedy plant species [J]. J. Chem. Ecol.,2000,26:303-313
111 Yu,J. Q. and Y. Matsui. Eeffcts of root exudates of cucumber and allelochemicals on ion uptake by cucumber seedlings[J].JChem. Ecol,1997,23:817-827
112 Politycka, B.. Free and glycosylated phenolics phenol-beta -glucosyltrnasferase activity and permabolity in cucumber roots effected by derivatives of cinnamic and benzic acids[J]. Acat.Physiologoae Plntarum,1997,19:311-317
113 Baziramakenga, R., Simard, R. R. and G. D. Leroux. Effects of benzoic and cinnamic acids on growth, mineral composition and chlorophyll content of soybean. [J]. J. Chem. Ecol, 1994,20:2821-833
114 Barkosky, R.R., Einhellig, F. A. and J. L. Butler. Caffeic acid-induced changes in plant-water Relationships and photosynthesis in leaf spurge Euophorbia esula[J]. JChem. Ecol., 2000,26:2095-2109
115 Boufalis,A. and F. Pellissier. Allelopathic effects of phenolic mixtures on respiration of two spruce mycorrhizal fungi [J]. JChem. Ecol,.1994,20:2283-2289
116 Einhellig, F. A., Rice,E. L., Rosser, P. G., et al. Effects of scopoletin on growth, C02exchange rate, and concentration scopoletin, scopolin, and pigweed[J]. Bull. Torry Bot. Club.1970,97:22-23
¨7宋莉.大蒜鳞茎粗提物对西瓜和黄瓜枯萎病的抑制效应及其机理研究[D].杨凌：西北农林科技大学,2006
118王宏乐.黄瓜不同抗性品种根分泌物及其对枯萎病病菌的影响[D].北京：中国农业科学院,2009
119邹芳斌,司龙亭,李新,等.黄瓜枯萎病抗性与防御系统几种酶活性关系的研究.华北农学报,2008,23(3)：181-184
120张书清.黄瓜枯萎病的识别与防治.农技服务,2007,(1)：59
121张卫艳.黄瓜黄萎病及枯萎病的鉴别与防治.农技服务,2007,24(10)：52
122 Franka, Einhellig, Gerald R Leather. Potentials for Exploiting Allelopathy to Enhance Crop Production. Journal of Chemical Ecology,1988,14(10):1829-1844
123 Putnam A. R. Allelopathic chemicals, Nature's herbicides in action. Chem and Eng News,1983,61(14):34-45
124曹阳.西芹腐根及腐根际区物浸提液对黄瓜枯萎病菌化感作用的研究[D].呼和浩特：内蒙古农业大学,2009
125张波,云兴福.西芹干根提取物对黄瓜枯萎病菌化感作用的研究.内蒙古农业大学学报(自然科学版),2010,31(1)：136-140
126贾俊英,张丽莹,云兴福西芹种子浸提液对黄瓜枯萎病菌的化感作用.生态学杂志,2011,30(7)：1473-1478
127贾俊英,马斌,孙鸿举,等.西芹种子浸提液处理后黄瓜叶片内生理指标变化的研究.华北农学报,2010,25(6)：144-149
128马斌,贾俊英,云兴福.西芹种子浸提液对黄瓜枯萎病菌化感作用机理的研究——黄瓜植株体内SOD、POD和CAT活性的变化.内蒙古农业大学学报(自然科学版),2010,31(2)：173-178
129阎飞,杨振明.大豆连作障碍中的生化互作作用[J].大豆科学,1998,17(2)：147-151
130王大力.水稻化感作用研究综述[J].生态学报,1998,18(3)：326-334
131阎飞,杨振明,韩丽梅.植物化感作用及其作用物的研究方法[J].生态学报,2000,20(7)：693-696
132梁文举,张晓珂,姜勇,等.根分泌的化感物质及其对土壤生物产生的影响[J].地球科学进展,2005,20(3)：330-334
133阎飞,杨振明,韩丽梅.论农业持续发展中的化感作用[J].应用生态学报,2001,12(4)：633-635
134韩建华.植物源抑菌活性物质初步研究[D].西北农林科技大学,2006
135宋炜.万寿菊根提取物对西瓜枯萎病菌的抑菌活性成分研究[D].山西农业大学,2005
136 MarcusL,Barash I, Sneh B, etal. Purification and characterization of pectolytic enzymes produced by virulent and hypovirulent isolates of Rhizoctonia solaniKuhn [J]. Physiol and Mol Plant Pathol,1986,29:325-326
137王学奎.植物生理生化实验原理和技术(第二版)[M].高等教育出版社,2006
138张凤芹,不同糖类对鸡腿菇胞外酶活性及多糖分泌的影响[J].食用菌.1999,21(4)：9
139李忠福,徐建国.分光光度法测定果胶酶活性方法的研究[J].黑龙江医药,2002,15：428-431
140石延霞,李宝聚.黄瓜霜霉病菌致病作用与两种细胞壁降解酶关系初探[J].园艺学报,2003,30(4)：465-466
141汪金莲,邱业先,陈宏伟等.茶多酚对几种植物病原真菌的抑制作用及机理研究[J].天然产物研究与开发,2008,20：690-694
142贾俊英.西芹种子浸提液对黄瓜枯萎病菌化感作用及其机理的研究[D].内蒙古农业大学,2011
143王金生.分子植物病理学.北京：中国农业出版社,1998,33-36
144张新虎,何静,沈慧敏.苍耳提取物对番茄灰霉病菌的抑制作用及抑菌机理初探[J].草业学报,2008,17(3)：99-104
145生理学实验指导[M].北京：中国农业出版社,2001
146 Sergiev I.Alexieva V, Karanove E. Effect of spermine, atrazine and combination between them on some endogenous protective systems and stress markers in plant [J]. Compt Rend Acid Bulg Sci,1997,51 (3):121-124
147 Gao J F. Zhi Wu Sheng Li Xue Shi Yan Ji Shu[M]. Xian: World Book Press.2000
148王爱国,罗广华.植物的超氧物自由基与羟氨反应的定量关系[J].植物生理学通讯1990,26(6)：55-57
149 Ingeborg Thoma, Christiane Loeffler, Alok K Sinha. Cycl.opentenone Isopros.tanes induced by reactive oxygen species trigger defense gene activation and phytoalexin accumulation in plants [J]. The plant J.,2003,34(3):363-368
150龚国强,于梁,周山涛.低温对黄瓜果实超氧化物歧化酶(SOD)的影响[J].园艺学报.1996,23(1)：97-98
151 Joseph LM, Tan TK, Wong SM, et a.l Antifungal effects of hydrogen peroxide and peroxidase on spore germination and mycelial grow ofPseudocercospora species [J]. Canadian Journal ofBotany,1998,76(12):2119-2124
152王玲平.黄瓜感染枯萎病菌后生理生化变化及其与抗病性关系的研究[D].山西农业大学.2001
153丁九敏,高洪斌,刘玉石等.黄瓜霜霉病抗性与叶片中生理生化物质含量关系的研究[J].辽宁农业科技.2005(1)：11-13
154潘汝谦,黄旭明,古希昕.活性氧清除酶类在黄瓜感染霜霉病过程中的活性变化[J].植物病理学报.1999,29(3)：287-288
155陈喜文,郝友进,陈德富,等.含氮杂环化合物对黄瓜白粉病抗性的诱导作用及防御酶系统的关系[J].植物病理学报.2003,33(6)：535-540
156徐建华,利容千,王建波.黄瓜不同抗病品种感染镰刀菌枯萎病菌后几种酶活性的变化[J].植物病理学报.1995,25(3)：239-242
157胡景江,刘志龙,文建雷.溃疡病菌低聚糖激发子诱导杨树细胞抗病机制的初步研究[J].西北农林科技大学学报(自然科学版),2003,31(4)：145-148
158周宝利,高艳新,林桂荣等.嫁接茄子抗病性与电导率、脯氨酸含量及苯丙氨酸解氨酶活性的关系[J].园艺学报.1998,25(3)：300-302
159李洪连等.黄瓜对炭疽病诱导抗性的初步研究-诱导抗病机的研究[J].植物病理学报.1993,23(4)：27-32
160李靖,利容千,袁文静黄瓜感染霜霉病菌叶片一些酶活性的变化[J].植物病理学报1991,21(4)：277-283
161 Schlunbaum A, Mauch F, Vogeli U, et al. Plant chitinases are potent inhibitors of fungal growth[J]. Nature,1986,324:365-367
162 Mauch F, Hadwiger L, Boller T. Antifungal hydrolases in peatissue I. Purification and characterization of two chitinases and two β-1,3-glucanases differentially regulated during development and in response to fungal infection[J]. Plant Physiol,1988a,87:325-333
163贾显禄,王振中,王平.水稻与稻瘟病菌非亲和性互作中重要防御酶活性变化规律研究[J].植物病理学报,2002,32(3)：206-213
164杜良成,王钧.稻瘟菌诱导的水稻几丁酶、β-1,3-葡聚糖酶活性及分布[J].植物生理学报,1992,18(1)：29-36
165 Cherif M,Asselin A, and Belanger R R. Defense responses induced by soluble silicon in cucumber roots infected by Pythium spp[J].Phytopathology,1994,84:236-242
166 Liang Y C, Sun W C, Si J,R?mheld V. Effect of foliar-and root-applied silicon on the enhancement of induced resistance in Cucumis sativus to powdery mildew[J]. Plant Pathology,2005,54:678-685
167 Luken R. J. Melting out ofKentucky bluegrass, a low sugar disease [J]. Phytopathology.1970,60: 1276-1278
168郑玉艳,周宝利,张淑红.苦参黄酮对辣椒枯萎病的抑制效应[J].沈阳农业大学学报,2009,39(3)：297-300
169王茹华,周宝利,张启发等.茄子根系分泌物中香草醛和肉桂酸对黄萎菌的化感效应[J].生态学报,2006,26(9)：3152-3155
170赵黎明,郑殿峰.不同植物生长调节剂对大豆叶片保护酶活性及同化物代谢的影响[J].华北农学报,2008,23(3)：106-111
171王建明,郭春绒,张作刚,等.西瓜不同品种苗期感染病菌后的生理生化变化[J].中国农业科学,2002,35(11)：1343-1348
172冯智富,王建明等.西瓜枯萎病菌毒素培养滤液对西瓜幼苗可溶性蛋白的影响[J].安徽农业科学,2008,36(10)：4162-4163
173曾永三,王振虹.豇豆与锈菌互作中的活性氧代谢研究[J].植物病理学报,2004,34(2)：146-152
174余文英.甘薯抗疮痂病的活性氧代谢、碳代谢与超微结构的研究[D].福建：福建农林大学,2003
175郭鸿儒.黄花蒿化感物质的分离鉴定及化感物质作用机理研究[D].兰州：甘肃农业大学,2007
176王树桐,曹克强,张凤巧.中药丁香提取物对番茄灰霉病菌抑制作用及生防效果[J].植物病理学报,2005,35(6)：91-94
177徐雅梅,呼天明.菊苣根提取物的抑菌活性研究[J].西北植物学报,2006,26(3)：615-619
178王冬梅,朱玮.卷叶黄精对植物病原菌的抗菌活性研究[J].西北植物学报,2006,26(7)：1473-1477
179韩丽梅,阎飞,王树起,等.重迎茬大豆根际土壤有机化合物的初步鉴定及对大豆种子萌发的化感作用[J].应用生态学报,2000,11(4)：582-586
180陈秋波,彭黎旭,贺利民,等.刚果12号桉树根及根际土壤中化感物质的成分分析[J].热带农业科学,2002,22(4)：28-34
181邵华,彭少麟,张驰,等.微甘菊的化感作用研究[J].生态学杂志,2003,22(5)：62-65
182郭兰萍,黄璐琦,蒋有绪,等.苍术根茎及根际土水提物生物活性研究及化感物质的鉴定[J].生态学报,2006,26(2)：528-535
183郜峰,洪冲,陈红歌,等.地黄根系分泌物生物活性研究及化感物质的鉴定[J].江西农业学报,2010,22(3)：131-134
184张爱加,袁照年,陈冬梅,等.甘蔗根际土壤化感潜力评价及其化感物质分析[J].中国生态农业学报,2010,18(5)：1013-1017
185何海滨,陈祥旭,林瑞余,等.化感水稻PI312777苗期根系分泌物中化学成分分析[J].应用生态学报,2005,16(12)：2383-2388
186廖张,张健,杨婉身,等.青花椒根际土壤化感物质成分分析[J].四川农业大学学报2006,24(1)：47-50
187 Lin W X, Kim K U, Smin D H.2000. Rice allelopathic potential and its modes of action on barnyardgrass (Echinochloa crus-galli)·Allelopathy,7(2):215-224
188 Gao J F. Zhi Wu Sheng Li Xue Shi Yan Ji Shu[M]. Xian: World Book Press.2000
189杨俊杰,陈利军,杨海霞,等.水杉种子挥发物质的鉴定及其抗菌活性测定[J].中国生态农业学报,2010,18(5)：1018-1021
190蒋继宏,李晓储,陈凤美,等.芳香性植物挥发油抑菌活性的研究[J].江苏林业科技,2004,31(3)：6-12
191崔南,南蓬,林满红,等.园柏和龙柏主要挥发物及其抑菌和杀菌作用[J].环境与健康杂志,2006.23(1)：63-65
192江贵波,陈实,曾任森.入侵物种三裂叶蟛蜞菊挥发物质的鉴定及其抗菌活性[J].中国生态农业学报,2008.16(4)：905-908
193田治国,王飞,刘洋,等.西北地区常见的6种植物挥发性物质抑菌效果研究[J].西北林学院学报,2009.24(6)：124-128
194郭阿君,王志英,邹丽.火炬树挥发性有机物释放动态及其抑菌作用[J].东北林业大学学报,2010,38(7)：103-104
195程智慧,金瑞,佟飞.蒜苗挥发物对黄瓜幼苗生长的化感效应研究[J].西北农业学报,2007,16(3)：149-152
196 Bridges S M. Distribution of ironcontaining superoxide dismutase in vascular plants[J].Plant Physiol,1981,68:275-278
197李美芹.壳聚糖抑制番茄叶霉病菌的活性与诱导抗性及其机理研究[D].北京：中国海洋大学,2007
198张晓鹿,薛泉宏,郭志英,等.混合接种对辣椒疫病生防菌定殖、辣椒生长及诱导抗性的影响[J].西北农林科技大学学报：自然科学版,2008,36(4)：151-158
199曾永三,王振中.苯丙氨酸解氨酶在植物抗病反应中的作用[J].仲恺农业技术学院学报,1999,12(3)：56-65
200王东明,贾媛崔继哲盐胁迫对植物的影响及植物盐适应性研究进展[J].中国农学通报,2009,25(4)：124-128
201陈磊,云兴福.西芹鲜根及根际区物化感物质成分鉴定[J].华北农学报,2012,27(2)：157-164
202陈磊,李蕾,项鹏宇,云兴福.西芹挥发物对黄瓜枯萎病菌的化感作用[J].生态学杂志,2012,31(4)：877-881
2 W ittackerR H, Feeny P P. Allelochemics:Chemical interactions between species [J]. Science,1971,171(3937):757-770
3孔垂华,徐涛,胡飞.胜红蓟化感作用研究Ⅱ.主要化感物质的释放途径和活性[J].应用生态学报,1998,9(3)：257-260
4 张艳杰,鲁顺保,高捍东.南方红豆杉种子不同溶剂提取液对白菜种子萌发及幼苗生长的化感效应[J].中国农学通报,2010,26(9)：190-194
5曲波,王承旭,陈旭辉,等.刺萼龙葵种子提取物对白菜种子及幼苗化感作用的研究[J].第三届全国生物入侵大会论文摘要集
6 肖春萍,刘博,杨莉,等.加拿大蓬种子生态适应性及其化感作用[J].吉林农业大学学报,2011,33(5)：5279-531
7 郭晓霞,沈益新,李志华.几种豆科牧草地上部水浸提液对稗草种子和幼苗的化感效应[J].草地学报,2006,14(4)：356-359
8 陈礼玲,庞珂佳,李同臣,等.角茴香根和地上部水浸液对杂草反枝苋的化感作用[J].西北林学院学报,2011,26(1)：138-142
9 刘建新,胡浩斌,王鑫.黄瓜地上部水浸液对番茄的化感抑制效应[J].中国生态农业学报,2009,17(2)：312-317
10张凤丽,周宝利,何雨.嫁接茄子地上部浸提液的化感作用研究[J].安徽农业科技,2010,38(5)：2302-2303
11彭晓邦,程飞,张硕新.核桃叶水浸液对不同产地黄芩的化感效应[J].草地学报,2011,19(5)：839-845
12韩志军,陈静,郑寒,等.花椒叶浸提液对大豆种子萌发和幼苗生长的化感作用[J].应用与环境生物学报,2011,17(4)：585-588
13孙志蓉,翟明普,贺润平,等.甘草柠条水浸物对种子萌发和幼苗生长的相互影响[J].中国中药杂志,2008,33(21)：2459-2463
14张汝民,王玉芝,侯平,等.几种牧草幼苗对冷蒿茎叶水浸提液化感作用的生理响应[J].生态学报,2010,30(8)：2197-2204
15李发林,黄炎和,张汉荣,等.几种水土保持型牧草叶浸提液化感作用效应[J].中国农学通报,2010,26(18)：401-405
16李美娜,李志华.紫花苜蓿茎叶浸提液对波斯婆婆纳化感效应的研究[J].中国草地学报,2010,32(1)：68-74
17王辉,谢永生,杨亚利,等.云雾山铁杆蒿茎叶浸提液对封育草地四种优势植物的化感效应[J].生态学报,2011,31(20)：6013-6021
18张凤娟,徐兴友,陈凤敏,等.黄顶菊茎叶浸提液对白菜和水稻幼苗化感作用的初步研究[J].西北植物学报,2008,28(8)：1669-1674
19陈业兵,王金信,彭学岗,等.银胶菊叶对茼麻和稗的化感作用[J].植物保护学报,2009,36(1)：77-81
20张敏,付冬梅,陈华保,等.紫茎泽兰叶片对小麦、油菜幼苗的化感作用及化感机制的初步研究[J].浙江大学学报(农业与生命科学版)2010,36(5)：547-553
21李志华,沈益新.红三叶茎叶对几种牧草种子及幼苗的化感作用[J].中国草地,2005,27(3)：41-43
22曹兵,宋丽华,张涛.臭椿根浸提液对刺槐幼苗生长的化感效应[J].西北农业学报,2009,18(3)：156-159
23李坤,郭修武,郭印山,等.葡萄根系浸提液的化感作用[J].应用生态学报,2010,21(7)：1779-1784
24李志华,沈益新.不同品种紫花苜蓿根水浸提液化感作用效应的研究[J].中国草地,2005,27(4)：39-46
25 Rice E L, Putnam A R,Tang C S. The Science of Allelopathy[M]. New York, John Wileye Sons,1986
26周凯,郭维明.加拿大一枝黄花根系和根际土壤水浸液对萝卜和白菜种子萌发及幼苗生长的影响[J].西北植物学报,2005,25(1)：174-178
27郭兰萍,黄璐琦,蒋有绪,等.不同化学型苍术根茎及根际土提取物生物活性及化感物质的比较[J].中国药学杂志,2006,41(10)：734-737
28郭兰萍,黄璐琦,蒋有绪,等.苍术根茎及根际土水提物生物活性研究及化感物质的鉴定[J].生态学报,2006,26(2)：528-535
29梁文举,张晓珂,姜勇等.根分泌的化感物质及其对土壤生物产生的影响[J].地球科学进展,2005,20(3).330-335
30 Rovira.A. D. et al.Plant root exudates[J]. Bot Rev.,1969,35-57
31 Vancura, V. Root exudates of plants[J].Plant and soil,1964,21:231-248
32切尔诺布里维卡,C.H.植物分泌物的生物学作用和间作中的种间相互关系[M].北京：科学出版社,1961
33张丽丽,王颖,王辉,等.侧柏根际土壤提取物对4种植物种子萌发和幼苗生长的化感效应[J].河北农业大学学报,2008,31(6)：83-86
34曹兵,宋丽华,张婷婷.臭椿根际土壤化感作用的初步研究[J].西北林学院学报,2009,24(4)：147-150
35董淑琦,马永清,税军峰,等.不同年代冬小麦品种根际土浸提液诱导小列当种子发芽的化感作用研究[J].中国农业大学学报,2009,14(2)：59-63
36张顺捷,马凤鸣,王玉波,等.大豆根系分泌物化感作用的初步研究[J].东北农业大学学报,2008,39(10)：1-5
37张凤丽,周宝利,王茹华,等.嫁接茄子根系分泌物的化感效应[J].应用生态学报,2005,16(4)：750-753
38董小艳,程智慧,张亮.百合根系分泌物对4种观赏植物的化感作用[J].西北农林科技大学学报(自然科学版),2008,36(9)：113-117
39周艳丽,程智慧,孟焕文.大蒜根系分泌物对不同受体蔬菜的化感作用[J].应用生态学报,2007,18(1)：81-86
40由海霞,梁银丽,吕文,等.不同作物根系分泌物对黄瓜的化感作用[J].西北农林科技大学学报(自然科学版),2006,34(6)：101-105
41陈淑芳,曹晓华.南瓜根系及根部土壤水浸液化感作用研究[J].热带作物学报,2011,32(12)：2268-2273
42 Molisch H. Der Einfluss einer Pflanze auf die andere -Allelopathie. Fischer, Jena.1937
43李绍文.生态生物化学[M].北京：北京大学出版社,2001
44 Muller C.H.,Muller W. H. and Haines B.L. Volatile growth inhibitors production by shrubs[J]. Science.1964,143:471-473
45江贵波,陈少雄,黄丹莹,等.夹竹桃挥发物的化感作用研究[J].江西农业学报,2008,20(7)：62-64
46刘小香,王真辉,陈秋波.巨尾桉挥发物化感作用初步研究[J].热带作物学报,2008,29(3)：338-341
47江贵波,曾任森.艾的挥发性物质化感作用研究[J].生态科学,2006,25(2)：106-108
48张晓芳,王金信,谢娜,等.白三叶草挥发物的化感作用及其化学成分分析[J].植物保护学报,2011,38(4)：374-378
49曹子林,王晓丽,杨桂荚.紫茎泽兰气态挥发物对云南松种子萌发及幼苗生长的化感作用[J].江西农业大学学报,2012,34(1)：77-81
50孔垂华,徐涛,胡飞.胜红蓟化感作用研究Ⅱ.主要化感物质的释放途径和活性[J],应用生态报,1998,9(3)：257-260
51黎华寿,黄京华,张修玉,等.香茅天然挥发物的化感作用及其化学成分分析[J].应用生态学报,2005,6(4)：763-767
52马瑞君,王明理,朱学泰,等.黄帚橐吾挥发物的化感作用及其主要成分分析[J].应用生态学报,2005,16(10)：1826-1829
53周志红,骆世明,牟子平.番茄(lycopersicon esculentum)的化感作用研究[J].应用生态学报,1997,8(4)：445-449
54程智慧,金瑞,佟飞.蒜苗挥发物对黄瓜幼苗生长的化感效应研究[J].西北农业学报,2007,16(3)：149-152
55马卫宾,颜霞,谢慧丽.小麦化感作用对非根际土壤微生物区系的影响初探[J].陕西农业科学,2007(4)：35-37
56沈荔花,郭琼霞,林文雄,等.加拿大一枝黄花对土壤微生物区系的影响研究[J].中国农学通报,2007,23(4)：323-327
57 Sturz A V, Christie B R, Matheson B G. Association of bacterial endophyte populations from red clover and potato crops with popential for beneficial allelopathy [J]. Can-J-Microbiol,1998,44(2):162-167
58 FrankA E, Gerald R L. Potentials forexploiting allelopathy to enhance crop product ion[J]. JChem Ecol,1988,14 (10):1829-1844
59李天昊.膜荚黄芪地上部水浸液对植物、昆虫及真菌的影响研究[D].吉林农业大学,2007
60朱文杰.入侵植物加拿大一枝黄花对土传病菌的化感作用[D].浙江大学,2011
61周礼胜.芒萁化感作用及抑菌试验研究[D].南昌大学,2007
62周宝利,陈志霞,杜亮,等.不同抗病性茄子根系分泌物对黄萎病的化感作用[J].生态学报,2011,31(14)：3964-3972
63李云鹏,周宝利,李之璞,等.野生抗病茄植株腐解物对茄子黄萎病菌的化感作用研究[J].沈阳农业大学学报,2007,38(4)：500-503
64 Larkin R P. Effect of successive watermelon plantings on Fusarium oxysporum and other microorganisms in soils suppressive and conductive to Fusarium Wilt of water melon[J]. Phytopathology,1993,83:1097-1104
65 Yang C H, Crowley D, E and Menge J A.165r DNA fingerprinting of rhizosphere baeterial communities assoeiated with healthy and phyto phorainfected avocadotoots[J].FEMS Mierobiol Eeol,2000,35:129-136
66吴凤芝,潘凯,周秀艳.苯丙烯酸对黄瓜幼苗生理特性的影响[J].应用生态学报,2005,16(5)：915-918
67 Li F M,Hu M Y. Isolation and characterization of a novel antialgal allelochemical from phragmites communis[J]. Environmental Microbiology,2005,71 (11):6545-6553
68 Liu DL,Lovett JV. Biologically active secondary metabolites of barley. Ⅱ. phytotoxicity of barley allelochemicals[J]. J Chem Ecol,1993,19(10):2231-2244
69孔垂华,胡飞.植物化感相生相克作用及其应用[J].北京：中国农业出版社.2001
70 Bais.Harsh P.,Vepachedu. Ramarao, Gilroy. Simon, et al. Allelopathy and Exotic Plant Invasion: From Molecules and Genes to Species Interactions[J]. Sicience.2003, 301 (5638):1377-1380
71 Penuelas J,Ribas-Carbo M, Giles L. Effects of allelochemicals on plant respiration and oxygen isotope fractionation by the alternative oxidase[J].J Chem Ecol,1996,22 (4): 801-805
72 Hejl A M, Einhellig F A., Rasmussen J A. Effects of juglone on growth, phyotosynthesis,and respiration[J]. Journal of Chemical Ecology,1993,19 (3):931-939
73 Einhellig F A, Rasmussen J A,Hejl A M, et al. Effects of roots exudates sorgoleone on photosynthesis. [J]. Journal of Chemical Ecology,1993,19:369-372
74 David S S.Chemistry and mechanisms of Allelopathic interactions[J]. Agronomy Journal,1996, 88:876-885
75陈冬梅,林文雄.水稻化感作用研究现状与展望[J].福建农业大学学报,2000,(3)：281-285
76陈伟,叶明志,周洁.植物中酚类物质的变化[J].福建农业大学学报,1997,26(4)：502-508
77李绍文.生态生物化学(二)：高等植物之间的生化关系[J].生态学杂志,1989,8(1)：66-70
78孔垂华,黄寿山,胡飞.胜红蓟化感作用研究V.挥发油对真菌、昆虫和植物的生物活性及其化学成份[J].生态学报,2001,21(4)：584-587
79喻景权,松井佳久.豌豆根系分泌物自毒作用的研究[J].园艺学报,1999,26(3)：175-179
80孔垂华,胡飞.植物化感(相生相克)作用及其应用.中国农业出版社,北京.2001
81 Inderjit & Dakshini, K M M. On laboratory bioassays in allelopathy[J]. The botanical review.1995,61(1):28-44
82 Tang C H &Yong C C. Collection and identification of allelopathic compands from the undisturbed root system of Bigalta limpogress (Hemarthria altissima) [J]. Plant Physiol.,1982,69:155-160
83 Whitehead D C, et al. Bound phenolic compounds in wheat extracts of soil, plant roots and leaf litter[J]. Soil Biol. Biochem.,1983,15 (2):133-136
84 Thomas S C Wang, et al. Soil phenolic acids as plant inhibitors[J]. Soil Sci.,1967, 103(4):239-246
85 Vance G F, et al. Phenolic compounds in soils of hydrosequences and developmental sequences of spodosols [J]. Soil Sci. Soc. Am J.,1986,50:992-996
86 Kuwatsuka S&Shindo H. Behavior of phenolic substances in the decaying process of plants (I). Soil Sci. Plant Nutr.,1973,19:219-227
87 Yu J Q &Yoshihisa M. Extraction and identification of phytotoxic substances accumulated in nutrient solution for hydroponic culture of tomato [J].Soil Sci. Plant Nutr.,1993, 39(4):691-700
88 Vance G F,et al.Extraction of phenolic compounds from a spodosol profile:an evaluation of three extractants[J]. Soil Sci.,1985,140 (6):412-420
89阎飞,杨振明,韩丽梅.植物化感作用及其作用物的研究方法[J].生态学报,2000,20(4)：692-696
90曾任森,林象联,等.蟛蜞菊的生化他感作用及生化他感作用物的分离鉴定[J].生态学报,1996,16(1)：20-27
91 Kagan I A,Rimando A M,Dayan F E. Chromatographic separation and in vitro activity of sorgoleone congeners from the roots of Sorghum bicolor[J]. Journal Agricultural and Food Chemistry,2003,51(26):7589-7595
92孔垂华,徐效华.有机物的分离和结构鉴定[M].北京：化学工业出版社,2003
93 Bulen W A, et al. Sepration of organic acids from plant tissues chromatographic technique [J].Anal. Chem.,1952,24:187-190
94 Guenzi W D&McCalla. Phenolic acids in oats,whear, sorghum, and corn residues and their phytotoxicityAgro. J.,1966,58:303-304
95马瑞霞,等.小麦根区微生物分解小麦残体产生的化感物质及其生物活性的研究[J].生态学报,1996,16(6)：632-639
96 Grierson P F.Organic acids in the rhizosphere ofBanksia integrifoliaL. F[J].Plant and Soil,1992,144:259-265
97刘秀芬,等.根际他感化学物质的分离鉴定与生物活性的研究[J].生态学报.1996,16(1)：1-10
98 Iderjit &Dakshini K M M. allelopathic potential of an annual weed Polypogon monspeliensis in crops in India[J].Plant and Soil,1995,173:251-257
99王明道,陈红歌,刘新育,等.地黄对芝麻的化感作用及其化感物质的分离鉴定[J].植物生态学报,2009,33(6)：1191-1198
100张爱加,袁照年,陈冬梅,等.甘蔗根系土壤化感潜力评价及其化感物质分析[J].中国生态农业学报,2010,18(5)：1013-1017
101耿广东,张素勤,程智慧.甘辣椒根系分泌物的化感作用及其化感物质分析[J].园艺学报,2009,36(6)：873-878
148 西芹根物质与挥发物对黄瓜枯萎病菌的化感作用、机理及化感物质分离纯化的研究
102陈业兵,王金信,吴小虎,等.银胶菊的花对稗草的化感作用及其化感物质分离与鉴定[J].植物保护学报,2010,37(1)：73-77
103曾任森.化感作用研究中的生物测定方法综述[J].应用生态学报,1999,10(1)：123-126
104 Leather G R,Einhellig F A.Bioassay in the study of allelopathy [A]. In:Putnam A R and Tang C S(ed).The Science of Allelopathy[C]. New York: John Willy and Sons,1986,133-145
105 Mortley D G,Loretan P A, Hill W A, et al. Biocompatibility of sweetpotato and peanut in a hydroponicsystem. Hortscience.1998,33(7):1147-1149
106 Einhellig, F. A. Mechanisms and modes of action of allelochemicals[M]. In:The Science of Allelopathy. A. R.Putnam and C. S. Tang (ed). John Wiley&Sons. New York.1986, 171-188
107 Politycka B.Phenolics and the acivities of phenylalanine ammonia-lyase, phenol-beta-glucosyl transferase and beta-glucosidase in cucumber roots as affected by phenolic allelochemicals[J]. Acta Physiologiae Plantarum,1998,20(4):405-410
108 Zobel, A. M. and J. M. Lynch. Extrusion of UV—absorbing phenolics in Acer. In response to UV and freezing temperatulre I. U VA—absorbing compounds on the surface of Acer saccharum and Acer platanoides autum leaves [J].Allelopathy Journal,1997,4:269-276
109 Calera, M. R., Anaya,A. L. and R. M. Gavilanes. Effects of phytotoxic resin glycoside on activity of H+-ATPase from plasma membrane[J]. J. Chem. Ecol.,1995,21:289-297
110 Romagni, J. G. and Allen, N. and F. E. Dayan. Allelopathic effects of volatile cineoles two weedy plant species [J]. J. Chem. Ecol.,2000,26:303-313
111 Yu,J. Q. and Y. Matsui. Eeffcts of root exudates of cucumber and allelochemicals on ion uptake by cucumber seedlings[J].JChem. Ecol,1997,23:817-827
112 Politycka, B.. Free and glycosylated phenolics phenol-beta -glucosyltrnasferase activity and permabolity in cucumber roots effected by derivatives of cinnamic and benzic acids[J]. Acat.Physiologoae Plntarum,1997,19:311-317
113 Baziramakenga, R., Simard, R. R. and G. D. Leroux. Effects of benzoic and cinnamic acids on growth, mineral composition and chlorophyll content of soybean. [J]. J. Chem. Ecol, 1994,20:2821-833
114 Barkosky, R.R., Einhellig, F. A. and J. L. Butler. Caffeic acid-induced changes in plant-water Relationships and photosynthesis in leaf spurge Euophorbia esula[J]. JChem. Ecol., 2000,26:2095-2109
115 Boufalis,A. and F. Pellissier. Allelopathic effects of phenolic mixtures on respiration of two spruce mycorrhizal fungi [J]. JChem. Ecol,.1994,20:2283-2289
116 Einhellig, F. A., Rice,E. L., Rosser, P. G., et al. Effects of scopoletin on growth, C02exchange rate, and concentration scopoletin, scopolin, and pigweed[J]. Bull. Torry Bot. Club.1970,97:22-23
¨7宋莉.大蒜鳞茎粗提物对西瓜和黄瓜枯萎病的抑制效应及其机理研究[D].杨凌：西北农林科技大学,2006
118王宏乐.黄瓜不同抗性品种根分泌物及其对枯萎病病菌的影响[D].北京：中国农业科学院,2009
119邹芳斌,司龙亭,李新,等.黄瓜枯萎病抗性与防御系统几种酶活性关系的研究.华北农学报,2008,23(3)：181-184
120张书清.黄瓜枯萎病的识别与防治.农技服务,2007,(1)：59
121张卫艳.黄瓜黄萎病及枯萎病的鉴别与防治.农技服务,2007,24(10)：52
122 Franka, Einhellig, Gerald R Leather. Potentials for Exploiting Allelopathy to Enhance Crop Production. Journal of Chemical Ecology,1988,14(10):1829-1844
123 Putnam A. R. Allelopathic chemicals, Nature's herbicides in action. Chem and Eng News,1983,61(14):34-45
124曹阳.西芹腐根及腐根际区物浸提液对黄瓜枯萎病菌化感作用的研究[D].呼和浩特：内蒙古农业大学,2009
125张波,云兴福.西芹干根提取物对黄瓜枯萎病菌化感作用的研究.内蒙古农业大学学报(自然科学版),2010,31(1)：136-140
126贾俊英,张丽莹,云兴福西芹种子浸提液对黄瓜枯萎病菌的化感作用.生态学杂志,2011,30(7)：1473-1478
127贾俊英,马斌,孙鸿举,等.西芹种子浸提液处理后黄瓜叶片内生理指标变化的研究.华北农学报,2010,25(6)：144-149
128马斌,贾俊英,云兴福.西芹种子浸提液对黄瓜枯萎病菌化感作用机理的研究——黄瓜植株体内SOD、POD和CAT活性的变化.内蒙古农业大学学报(自然科学版),2010,31(2)：173-178
129阎飞,杨振明.大豆连作障碍中的生化互作作用[J].大豆科学,1998,17(2)：147-151
130王大力.水稻化感作用研究综述[J].生态学报,1998,18(3)：326-334
131阎飞,杨振明,韩丽梅.植物化感作用及其作用物的研究方法[J].生态学报,2000,20(7)：693-696
132梁文举,张晓珂,姜勇,等.根分泌的化感物质及其对土壤生物产生的影响[J].地球科学进展,2005,20(3)：330-334
133阎飞,杨振明,韩丽梅.论农业持续发展中的化感作用[J].应用生态学报,2001,12(4)：633-635
134韩建华.植物源抑菌活性物质初步研究[D].西北农林科技大学,2006
135宋炜.万寿菊根提取物对西瓜枯萎病菌的抑菌活性成分研究[D].山西农业大学,2005
136 MarcusL,Barash I, Sneh B, etal. Purification and characterization of pectolytic enzymes produced by virulent and hypovirulent isolates of Rhizoctonia solaniKuhn [J]. Physiol and Mol Plant Pathol,1986,29:325-326
137王学奎.植物生理生化实验原理和技术(第二版)[M].高等教育出版社,2006
138张凤芹,不同糖类对鸡腿菇胞外酶活性及多糖分泌的影响[J].食用菌.1999,21(4)：9
139李忠福,徐建国.分光光度法测定果胶酶活性方法的研究[J].黑龙江医药,2002,15：428-431
140石延霞,李宝聚.黄瓜霜霉病菌致病作用与两种细胞壁降解酶关系初探[J].园艺学报,2003,30(4)：465-466
141汪金莲,邱业先,陈宏伟等.茶多酚对几种植物病原真菌的抑制作用及机理研究[J].天然产物研究与开发,2008,20：690-694
142贾俊英.西芹种子浸提液对黄瓜枯萎病菌化感作用及其机理的研究[D].内蒙古农业大学,2011
143王金生.分子植物病理学.北京：中国农业出版社,1998,33-36
144张新虎,何静,沈慧敏.苍耳提取物对番茄灰霉病菌的抑制作用及抑菌机理初探[J].草业学报,2008,17(3)：99-104
145生理学实验指导[M].北京：中国农业出版社,2001
146 Sergiev I.Alexieva V, Karanove E. Effect of spermine, atrazine and combination between them on some endogenous protective systems and stress markers in plant [J]. Compt Rend Acid Bulg Sci,1997,51 (3):121-124
147 Gao J F. Zhi Wu Sheng Li Xue Shi Yan Ji Shu[M]. Xian: World Book Press.2000
148王爱国,罗广华.植物的超氧物自由基与羟氨反应的定量关系[J].植物生理学通讯1990,26(6)：55-57
149 Ingeborg Thoma, Christiane Loeffler, Alok K Sinha. Cycl.opentenone Isopros.tanes induced by reactive oxygen species trigger defense gene activation and phytoalexin accumulation in plants [J]. The plant J.,2003,34(3):363-368
150龚国强,于梁,周山涛.低温对黄瓜果实超氧化物歧化酶(SOD)的影响[J].园艺学报.1996,23(1)：97-98
151 Joseph LM, Tan TK, Wong SM, et a.l Antifungal effects of hydrogen peroxide and peroxidase on spore germination and mycelial grow ofPseudocercospora species [J]. Canadian Journal ofBotany,1998,76(12):2119-2124
152王玲平.黄瓜感染枯萎病菌后生理生化变化及其与抗病性关系的研究[D].山西农业大学.2001
153丁九敏,高洪斌,刘玉石等.黄瓜霜霉病抗性与叶片中生理生化物质含量关系的研究[J].辽宁农业科技.2005(1)：11-13
154潘汝谦,黄旭明,古希昕.活性氧清除酶类在黄瓜感染霜霉病过程中的活性变化[J].植物病理学报.1999,29(3)：287-288
155陈喜文,郝友进,陈德富,等.含氮杂环化合物对黄瓜白粉病抗性的诱导作用及防御酶系统的关系[J].植物病理学报.2003,33(6)：535-540
156徐建华,利容千,王建波.黄瓜不同抗病品种感染镰刀菌枯萎病菌后几种酶活性的变化[J].植物病理学报.1995,25(3)：239-242
157胡景江,刘志龙,文建雷.溃疡病菌低聚糖激发子诱导杨树细胞抗病机制的初步研究[J].西北农林科技大学学报(自然科学版),2003,31(4)：145-148
158周宝利,高艳新,林桂荣等.嫁接茄子抗病性与电导率、脯氨酸含量及苯丙氨酸解氨酶活性的关系[J].园艺学报.1998,25(3)：300-302
159李洪连等.黄瓜对炭疽病诱导抗性的初步研究-诱导抗病机的研究[J].植物病理学报.1993,23(4)：27-32
160李靖,利容千,袁文静黄瓜感染霜霉病菌叶片一些酶活性的变化[J].植物病理学报1991,21(4)：277-283
161 Schlunbaum A, Mauch F, Vogeli U, et al. Plant chitinases are potent inhibitors of fungal growth[J]. Nature,1986,324:365-367
162 Mauch F, Hadwiger L, Boller T. Antifungal hydrolases in peatissue I. Purification and characterization of two chitinases and two β-1,3-glucanases differentially regulated during development and in response to fungal infection[J]. Plant Physiol,1988a,87:325-333
163贾显禄,王振中,王平.水稻与稻瘟病菌非亲和性互作中重要防御酶活性变化规律研究[J].植物病理学报,2002,32(3)：206-213
164杜良成,王钧.稻瘟菌诱导的水稻几丁酶、β-1,3-葡聚糖酶活性及分布[J].植物生理学报,1992,18(1)：29-36
165 Cherif M,Asselin A, and Belanger R R. Defense responses induced by soluble silicon in cucumber roots infected by Pythium spp[J].Phytopathology,1994,84:236-242
166 Liang Y C, Sun W C, Si J,R?mheld V. Effect of foliar-and root-applied silicon on the enhancement of induced resistance in Cucumis sativus to powdery mildew[J]. Plant Pathology,2005,54:678-685
167 Luken R. J. Melting out ofKentucky bluegrass, a low sugar disease [J]. Phytopathology.1970,60: 1276-1278
168郑玉艳,周宝利,张淑红.苦参黄酮对辣椒枯萎病的抑制效应[J].沈阳农业大学学报,2009,39(3)：297-300
169王茹华,周宝利,张启发等.茄子根系分泌物中香草醛和肉桂酸对黄萎菌的化感效应[J].生态学报,2006,26(9)：3152-3155
170赵黎明,郑殿峰.不同植物生长调节剂对大豆叶片保护酶活性及同化物代谢的影响[J].华北农学报,2008,23(3)：106-111
171王建明,郭春绒,张作刚,等.西瓜不同品种苗期感染病菌后的生理生化变化[J].中国农业科学,2002,35(11)：1343-1348
172冯智富,王建明等.西瓜枯萎病菌毒素培养滤液对西瓜幼苗可溶性蛋白的影响[J].安徽农业科学,2008,36(10)：4162-4163
173曾永三,王振虹.豇豆与锈菌互作中的活性氧代谢研究[J].植物病理学报,2004,34(2)：146-152
174余文英.甘薯抗疮痂病的活性氧代谢、碳代谢与超微结构的研究[D].福建：福建农林大学,2003
175郭鸿儒.黄花蒿化感物质的分离鉴定及化感物质作用机理研究[D].兰州：甘肃农业大学,2007
176王树桐,曹克强,张凤巧.中药丁香提取物对番茄灰霉病菌抑制作用及生防效果[J].植物病理学报,2005,35(6)：91-94
177徐雅梅,呼天明.菊苣根提取物的抑菌活性研究[J].西北植物学报,2006,26(3)：615-619
178王冬梅,朱玮.卷叶黄精对植物病原菌的抗菌活性研究[J].西北植物学报,2006,26(7)：1473-1477
179韩丽梅,阎飞,王树起,等.重迎茬大豆根际土壤有机化合物的初步鉴定及对大豆种子萌发的化感作用[J].应用生态学报,2000,11(4)：582-586
180陈秋波,彭黎旭,贺利民,等.刚果12号桉树根及根际土壤中化感物质的成分分析[J].热带农业科学,2002,22(4)：28-34
181邵华,彭少麟,张驰,等.微甘菊的化感作用研究[J].生态学杂志,2003,22(5)：62-65
182郭兰萍,黄璐琦,蒋有绪,等.苍术根茎及根际土水提物生物活性研究及化感物质的鉴定[J].生态学报,2006,26(2)：528-535
183郜峰,洪冲,陈红歌,等.地黄根系分泌物生物活性研究及化感物质的鉴定[J].江西农业学报,2010,22(3)：131-134
184张爱加,袁照年,陈冬梅,等.甘蔗根际土壤化感潜力评价及其化感物质分析[J].中国生态农业学报,2010,18(5)：1013-1017
185何海滨,陈祥旭,林瑞余,等.化感水稻PI312777苗期根系分泌物中化学成分分析[J].应用生态学报,2005,16(12)：2383-2388
186廖张,张健,杨婉身,等.青花椒根际土壤化感物质成分分析[J].四川农业大学学报2006,24(1)：47-50
187 Lin W X, Kim K U, Smin D H.2000. Rice allelopathic potential and its modes of action on barnyardgrass (Echinochloa crus-galli)·Allelopathy,7(2):215-224
188 Gao J F. Zhi Wu Sheng Li Xue Shi Yan Ji Shu[M]. Xian: World Book Press.2000
189杨俊杰,陈利军,杨海霞,等.水杉种子挥发物质的鉴定及其抗菌活性测定[J].中国生态农业学报,2010,18(5)：1018-1021
190蒋继宏,李晓储,陈凤美,等.芳香性植物挥发油抑菌活性的研究[J].江苏林业科技,2004,31(3)：6-12
191崔南,南蓬,林满红,等.园柏和龙柏主要挥发物及其抑菌和杀菌作用[J].环境与健康杂志,2006.23(1)：63-65
192江贵波,陈实,曾任森.入侵物种三裂叶蟛蜞菊挥发物质的鉴定及其抗菌活性[J].中国生态农业学报,2008.16(4)：905-908
193田治国,王飞,刘洋,等.西北地区常见的6种植物挥发性物质抑菌效果研究[J].西北林学院学报,2009.24(6)：124-128
194郭阿君,王志英,邹丽.火炬树挥发性有机物释放动态及其抑菌作用[J].东北林业大学学报,2010,38(7)：103-104
195程智慧,金瑞,佟飞.蒜苗挥发物对黄瓜幼苗生长的化感效应研究[J].西北农业学报,2007,16(3)：149-152
196 Bridges S M. Distribution of ironcontaining superoxide dismutase in vascular plants[J].Plant Physiol,1981,68:275-278
197李美芹.壳聚糖抑制番茄叶霉病菌的活性与诱导抗性及其机理研究[D].北京：中国海洋大学,2007
198张晓鹿,薛泉宏,郭志英,等.混合接种对辣椒疫病生防菌定殖、辣椒生长及诱导抗性的影响[J].西北农林科技大学学报：自然科学版,2008,36(4)：151-158
199曾永三,王振中.苯丙氨酸解氨酶在植物抗病反应中的作用[J].仲恺农业技术学院学报,1999,12(3)：56-65
200王东明,贾媛崔继哲盐胁迫对植物的影响及植物盐适应性研究进展[J].中国农学通报,2009,25(4)：124-128
201陈磊,云兴福.西芹鲜根及根际区物化感物质成分鉴定[J].华北农学报,2012,27(2)：157-164
202陈磊,李蕾,项鹏宇,云兴福.西芹挥发物对黄瓜枯萎病菌的化感作用[J].生态学杂志,2012,31(4)：877-881