早期运动干预促进缺血性脑损伤神经系统功能恢复的实验与临床对比研究
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摘要
缺血性脑损伤是临床上常见的疾病之一,可引起脑神经组织细胞缺血,水肿和坏死,导致一系列的症状、体征,甚至出现不可逆的脑损害,可留下各种神经系统后遗症。为了探讨早期运动干预对缺血性脑损伤神经运动功能恢复的促进作用,本研究采取基础与临床对比研究的方法,通过观察早期运动训练,对缺血再灌注( Middle Cerebral Artery Occlusion,MCAO)模型脑缺血大鼠恢复期脑组织微管相关蛋白-2含量的影响和大鼠行为的变化,来判断早期运动对脑损伤动物功能恢复的作用;同时,以临床高危新生儿缺血性脑损伤恢复期不同阶段的智力发育指数( Mental Development Index, MDI)和运动发育指数( Psychomotive Development Index,PDI)为指标,来观察早期运动干预对早产低体重儿、缺氧缺血性脑病神经系统功能恢复的促进作用。
微管相关蛋白-2(Microtuble-associated Protein 2,MAP-2)属于结构性相关蛋白家族,是一种热稳定的鳞蛋白,主要存在于神经元胞体和树突,是脑内最丰富的蛋白之一。它与微管连接促进微管的稳定,构成细胞骨架,参与神经元发育、结构稳定,突起形成和突触可塑性调节。
MAP-2与缺血性脑损伤关系密切。大量研究表明,MAP-2是脑缺血的敏感指标,而且进一步证实,MAP-2不仅是神经元损伤的早期标志,也与缺血半暗带受损神经元的重建相联系。脑缺血后MAP-2丢失的机制目前还不完全清楚,可能是脑缺血后引起神经元细胞膜去极化,兴奋性氨基酸(EAA)释放,继之激活EAA受体,Ca2+通过NMDA和非NMDA受体内流从而增加细胞内游离钙水平,破坏钙稳态,Ca2+同钙调节蛋白结合,激活靶点酶,致MAP-2异常鳞酸化,从而抑制微管组装,促进解聚,最终MAP-2丢失。
MAP-2主要表达在神经元胞体、树突和树突棘,与突触后膜骨架上一些信号蛋白和膜受体相连,是许多神经信号传导通路的底物,调节神经元突触可塑性以及细胞骨架动力学,对于神经功能是必须的。神经元-MAP-2的丢失涉及到神经元结构破坏和导致神经功能障碍的神经元死亡,通过检测干预后脑内MAP-2的含量,可间接了解脑神经功能的状态及干预的效果。
新生儿缺血缺氧性脑病(Hypoxic Ischemic Encephalopathy,HIE)是指围产儿因宫内窒息或出生后多种心脑疾病引起的脑严重缺血缺氧性损害,其脑组织以水肿、软化和坏死出血为主要病变,临床上病情重,病死率和致残率高,可发生多种永久性神经功能缺陷如智力低下、痉挛性瘫痪和癫痫等。它不仅可引起围产期新生儿死亡,而且是新生儿期以后致残的主要原因。HIE发病机制复杂,尚未完全明了,包括脑部能量代谢障碍、再灌注损伤、细胞凋亡、钙平衡紊乱、氧自由基产生、兴奋性氨基酸增多等。
本研究旨在通过基础与临床的结合对比研究,探讨早期运动干预对促进脑损伤神经系统功能恢复、降低后遗症的作用效果,为临床治疗缺血性脑疾病提供动物实验与临床依据。
本研究结果显示:
1、在MCAO模型动物实验中,缺血再灌注运动组大鼠行为学测试评分较单纯缺血再灌注组在术后2小时及24小时后略低,7日后有所升高;在14日和21日测试则显著增高,两组间差别具有极显著性统计学意义。
2、MCAO模型制成后,脑缺血再灌注运动组及单纯缺血再灌注组在缺血中心区的MAP-2免疫阳性物质减少或缺失,缺血周边区免疫阳性物质增高。但实验至第二、第三周时,缺血再灌注运动组缺血区MAP-2免疫阳性物质显著高于单纯缺血再灌注对照组,差别有极显著性统计学意义。
3、早期综合干预的早产、低出生体重高危儿组,其MDI比较在6月龄时干预组较常规组高1.6分,但差异无显著性;在12月龄、18月龄和24月龄时,干预组较常规组分别高2.2分、7.6分和7.7分,差别均有极显著性意义(P<0.01)。PDI比较在6、12、18和24月龄时干预组较常规组分别高3.8分、9.1分、12.2分和12.6分,各月龄比较差异均有极显著性意义(P<0.01)。
4、早期运动干预的HIE组与对照组比较,分别于6月、12月进行MDI和PDI测查,结果显示生后6月两组比较,MDI无显著性差别,而PDI差别有显著性;生后12月两组比较,干预组MDI、PDI均较对照组有显著性增高,差别有极显著性统计学意义(P<0.01)。
结论:
1、早期运动训练能促进未成年MCAO大鼠的肢体功能恢复,上调脑组织缺血皮质区MAP-2的表达,从分子水平上提示大鼠的肢体功能恢复可能与神经再生有关。
2、早期综合运动干预能够明显改善脑损伤高危新生儿和HIE患儿的预后,促进神经再生和脑功能的恢复,降低神经系统后遗症的发生,从而提高脑损伤新生儿患者的生存质量。
3、早期综合运动干预可以明显改善早产、低出生体重儿的智能发育。通过早期动作和运动能力的强化训练,可以大大提高早产、低出生体重儿的运动发育并进一步提高其智力发育。
4、早期运动干预效果确切,家庭与门诊相结合的干预模式值得在基层儿童保健部门及社区推广和应用,为提高人口素质提供保证。
Ischemic brain injure is one of the common clinical diseases and often caused brain nerve tissue cell ischemic, odema, necrosis , result in serial symptoms and signs. Even appeared nonreversiber brain damage, can leave behind various kinds of nervous system sequelae. For approach the effection of early exercise intervention for ischemic brain injure nervous system functional recovery , This study take the method of contrasting experiment and clinical effect by observe early movement training for ischemic reperfusion rat model brain ischemic recovery stage brain tissue microtubule-2 content effect and rat behavioral change, to juge early movement effect for functional recovery of brain injury animal. At the same time, take the MDI, PDI for index in different stage of clinical high risk neonate ischemic brain injury cases. To observe the effect of promotion for premature and low birth weight、HIE nervous system function recovery.
MAP -2 belong to structure-associated protein family clan, it is an heat-stable lipid protein, mainly reside in neuron body and dendrite. It is one of the most profused protein in the brain. It link with microtubule to promote microtubule stable, to constitute cytokelet, participate neuron development and structure stable,to form ecptoma and synapse plasticity adjustment.
MAP-2 has a intimate relationship with ischemic brain injury. A lot of studies indicate that MAP-2 is a sensitive mark for brain ischemic. Moreover confirmed, MAP-2 is not only the early mark for neuron injury, but also related to re-establish of damage neuron in ischemic semidarkness band. The mechanism for MAP-2 loss after brain ischemic is not clear at present, maybe brain ischemic cause neuron membrane de-pole, excitatory amino acids(EAA) release, continue activation EAA receptor, ca++ increase endocell liberation calcium level by NMDA and non-NMDA receptor inflow, to destroy calcium homeostasis, cause MAP-2 anomaly lipid acidize, Thus inhibition microtubule construction, promote depolymerize, MAP-2 lost at finally.
MAP-2 mainly expressed in pericargon, dendrite, dendritic spine, connected with signal protein and membrance receptor at postsynaptic membrance skeleton. It is substrate of conduction way for many nerves signals, adjust neuron synaptic plasticity and cystoskeleton dynamics. It is imperative for nerves function. The lost of neuron MAP-2 is related to neuron disorganization and lead to death of handicapped neuron. By detect MAP-2 content inside brain after intervention, we can get the message indirectly about cranial nerve function condition and the result of intervention.
HIE is refers to serious ischemic and hypoxic brain damage because of intrauterine asphyxia or many kinds diseases of heart and brain postnatal. The main pathological changes about brain tissue is oedema, lenition and necrosis, hemorrhage. It has heavy clinical condition, high case fatality rate and mutilation rate, can cause many kinds of permanence nerves functional defect, such as mental retardation, spastic paralysis and epilepsy. It not only cause neonatal death ambibirth period, but also main causies for mutilation after neonatal period. The pathogenesis for HIE is complicated,still not completely understood. Include brain energy disbolism, reperfusion injury, apoptosis, calcium balance confused, oxygen free radical produced, excitatory amino acids increased.
The purpose for this study is a contrast study by combination of animal experiment and clinical case, to approach the result of promote nervous system function recovery by early exercise intervention, and provide theory and clinical evidence for clinical therapy of ischemic brain diseases.
The finding of this study shows:
1. In MCAO model animal experiment, the behavious test grade for ischemic reperfusion exercise group rat is a little lower than purely ischemic reperfusion group in 2 hours and 24 hours after operation but increased 7 days later; the test grade much higher in 14 and 21 days. The difference between two groups has extremely statistical significance.
2. After MCAO model finished, the MAP-2 immune positive material decreased or absence at ischemic center zone both in brain ischemic reperfusion exercise group and purely ischemic reperfusion group, but immune positive materials at ischemic perimeter zone increased. The MAP-2 immune positive materials much higher at ischemic zone in ischemic reperfusion exercise group than in purely ischemic reperfusion group. The difference between two groups has extremely statistical significance.
3. In premature and low birth weight high risk infants group by early synthetic intervention, It’s MDI score 1.6 high than routine group in 6 months. There is no significant difference between two groups; Intervention group score 2.2, 7.6 and 7.7 higher than routine group during 12, 18 and 24 month age; the difference have extremely statistical significance. For PDI contrast, intervention group score 3.8, 9.1 12.2 and 12.6 higher respectively than routine group during 6, 12 18 and 24 months age. All months age compared have extremely statistical significance.(p<0.01)
4. Compare early exercise intervention HIE group with control group, check their MDI and PDI in 6 and 12 months age, the results shows MDI has no significance difference in 6 months age but PDI do has. In 12 months age , both MDI and PDI in intervention group much higher than control group. The difference have extremely statistical significance.(P<0.01)
Conclusion:
1. Early exercise training can promote limbs function recovery for minor MCAO rats, re-regulation MAP-2 express at ischemic cortical area in brain tissue. It suggest that rat limbs function recovery maybe related to nerve regeneration in molecular level.
2. Early synthetic movement intervention can obviously improve the prognosis of brain injuried high risk neonates and HIE patients, promote nerve regeneration and recovery of brain function, cut down nervous system sequel, thus elevate life quality for brain injury neonates.
3. Early synthetic movement intervention can obviously improve mental development for premature and low birth weight neonates. By enrichment training of earlier start moving and motor capacity, can obviously improve motor development and enhance mental development for premature and low birth weight neonates.
4. Early exercise intervention has a certain effect. It is worth spreading and utilizing in the basic level child care department and community by family connective clinic service intervention mode, and provide supports for elevation population diathesis.
微管相关蛋白-2(Microtuble-associated Protein 2,MAP-2)属于结构性相关蛋白家族,是一种热稳定的鳞蛋白,主要存在于神经元胞体和树突,是脑内最丰富的蛋白之一。它与微管连接促进微管的稳定,构成细胞骨架,参与神经元发育、结构稳定,突起形成和突触可塑性调节。
MAP-2与缺血性脑损伤关系密切。大量研究表明,MAP-2是脑缺血的敏感指标,而且进一步证实,MAP-2不仅是神经元损伤的早期标志,也与缺血半暗带受损神经元的重建相联系。脑缺血后MAP-2丢失的机制目前还不完全清楚,可能是脑缺血后引起神经元细胞膜去极化,兴奋性氨基酸(EAA)释放,继之激活EAA受体,Ca2+通过NMDA和非NMDA受体内流从而增加细胞内游离钙水平,破坏钙稳态,Ca2+同钙调节蛋白结合,激活靶点酶,致MAP-2异常鳞酸化,从而抑制微管组装,促进解聚,最终MAP-2丢失。
MAP-2主要表达在神经元胞体、树突和树突棘,与突触后膜骨架上一些信号蛋白和膜受体相连,是许多神经信号传导通路的底物,调节神经元突触可塑性以及细胞骨架动力学,对于神经功能是必须的。神经元-MAP-2的丢失涉及到神经元结构破坏和导致神经功能障碍的神经元死亡,通过检测干预后脑内MAP-2的含量,可间接了解脑神经功能的状态及干预的效果。
新生儿缺血缺氧性脑病(Hypoxic Ischemic Encephalopathy,HIE)是指围产儿因宫内窒息或出生后多种心脑疾病引起的脑严重缺血缺氧性损害,其脑组织以水肿、软化和坏死出血为主要病变,临床上病情重,病死率和致残率高,可发生多种永久性神经功能缺陷如智力低下、痉挛性瘫痪和癫痫等。它不仅可引起围产期新生儿死亡,而且是新生儿期以后致残的主要原因。HIE发病机制复杂,尚未完全明了,包括脑部能量代谢障碍、再灌注损伤、细胞凋亡、钙平衡紊乱、氧自由基产生、兴奋性氨基酸增多等。
本研究旨在通过基础与临床的结合对比研究,探讨早期运动干预对促进脑损伤神经系统功能恢复、降低后遗症的作用效果,为临床治疗缺血性脑疾病提供动物实验与临床依据。
本研究结果显示:
1、在MCAO模型动物实验中,缺血再灌注运动组大鼠行为学测试评分较单纯缺血再灌注组在术后2小时及24小时后略低,7日后有所升高;在14日和21日测试则显著增高,两组间差别具有极显著性统计学意义。
2、MCAO模型制成后,脑缺血再灌注运动组及单纯缺血再灌注组在缺血中心区的MAP-2免疫阳性物质减少或缺失,缺血周边区免疫阳性物质增高。但实验至第二、第三周时,缺血再灌注运动组缺血区MAP-2免疫阳性物质显著高于单纯缺血再灌注对照组,差别有极显著性统计学意义。
3、早期综合干预的早产、低出生体重高危儿组,其MDI比较在6月龄时干预组较常规组高1.6分,但差异无显著性;在12月龄、18月龄和24月龄时,干预组较常规组分别高2.2分、7.6分和7.7分,差别均有极显著性意义(P<0.01)。PDI比较在6、12、18和24月龄时干预组较常规组分别高3.8分、9.1分、12.2分和12.6分,各月龄比较差异均有极显著性意义(P<0.01)。
4、早期运动干预的HIE组与对照组比较,分别于6月、12月进行MDI和PDI测查,结果显示生后6月两组比较,MDI无显著性差别,而PDI差别有显著性;生后12月两组比较,干预组MDI、PDI均较对照组有显著性增高,差别有极显著性统计学意义(P<0.01)。
结论:
1、早期运动训练能促进未成年MCAO大鼠的肢体功能恢复,上调脑组织缺血皮质区MAP-2的表达,从分子水平上提示大鼠的肢体功能恢复可能与神经再生有关。
2、早期综合运动干预能够明显改善脑损伤高危新生儿和HIE患儿的预后,促进神经再生和脑功能的恢复,降低神经系统后遗症的发生,从而提高脑损伤新生儿患者的生存质量。
3、早期综合运动干预可以明显改善早产、低出生体重儿的智能发育。通过早期动作和运动能力的强化训练,可以大大提高早产、低出生体重儿的运动发育并进一步提高其智力发育。
4、早期运动干预效果确切,家庭与门诊相结合的干预模式值得在基层儿童保健部门及社区推广和应用,为提高人口素质提供保证。
Ischemic brain injure is one of the common clinical diseases and often caused brain nerve tissue cell ischemic, odema, necrosis , result in serial symptoms and signs. Even appeared nonreversiber brain damage, can leave behind various kinds of nervous system sequelae. For approach the effection of early exercise intervention for ischemic brain injure nervous system functional recovery , This study take the method of contrasting experiment and clinical effect by observe early movement training for ischemic reperfusion rat model brain ischemic recovery stage brain tissue microtubule-2 content effect and rat behavioral change, to juge early movement effect for functional recovery of brain injury animal. At the same time, take the MDI, PDI for index in different stage of clinical high risk neonate ischemic brain injury cases. To observe the effect of promotion for premature and low birth weight、HIE nervous system function recovery.
MAP -2 belong to structure-associated protein family clan, it is an heat-stable lipid protein, mainly reside in neuron body and dendrite. It is one of the most profused protein in the brain. It link with microtubule to promote microtubule stable, to constitute cytokelet, participate neuron development and structure stable,to form ecptoma and synapse plasticity adjustment.
MAP-2 has a intimate relationship with ischemic brain injury. A lot of studies indicate that MAP-2 is a sensitive mark for brain ischemic. Moreover confirmed, MAP-2 is not only the early mark for neuron injury, but also related to re-establish of damage neuron in ischemic semidarkness band. The mechanism for MAP-2 loss after brain ischemic is not clear at present, maybe brain ischemic cause neuron membrane de-pole, excitatory amino acids(EAA) release, continue activation EAA receptor, ca++ increase endocell liberation calcium level by NMDA and non-NMDA receptor inflow, to destroy calcium homeostasis, cause MAP-2 anomaly lipid acidize, Thus inhibition microtubule construction, promote depolymerize, MAP-2 lost at finally.
MAP-2 mainly expressed in pericargon, dendrite, dendritic spine, connected with signal protein and membrance receptor at postsynaptic membrance skeleton. It is substrate of conduction way for many nerves signals, adjust neuron synaptic plasticity and cystoskeleton dynamics. It is imperative for nerves function. The lost of neuron MAP-2 is related to neuron disorganization and lead to death of handicapped neuron. By detect MAP-2 content inside brain after intervention, we can get the message indirectly about cranial nerve function condition and the result of intervention.
HIE is refers to serious ischemic and hypoxic brain damage because of intrauterine asphyxia or many kinds diseases of heart and brain postnatal. The main pathological changes about brain tissue is oedema, lenition and necrosis, hemorrhage. It has heavy clinical condition, high case fatality rate and mutilation rate, can cause many kinds of permanence nerves functional defect, such as mental retardation, spastic paralysis and epilepsy. It not only cause neonatal death ambibirth period, but also main causies for mutilation after neonatal period. The pathogenesis for HIE is complicated,still not completely understood. Include brain energy disbolism, reperfusion injury, apoptosis, calcium balance confused, oxygen free radical produced, excitatory amino acids increased.
The purpose for this study is a contrast study by combination of animal experiment and clinical case, to approach the result of promote nervous system function recovery by early exercise intervention, and provide theory and clinical evidence for clinical therapy of ischemic brain diseases.
The finding of this study shows:
1. In MCAO model animal experiment, the behavious test grade for ischemic reperfusion exercise group rat is a little lower than purely ischemic reperfusion group in 2 hours and 24 hours after operation but increased 7 days later; the test grade much higher in 14 and 21 days. The difference between two groups has extremely statistical significance.
2. After MCAO model finished, the MAP-2 immune positive material decreased or absence at ischemic center zone both in brain ischemic reperfusion exercise group and purely ischemic reperfusion group, but immune positive materials at ischemic perimeter zone increased. The MAP-2 immune positive materials much higher at ischemic zone in ischemic reperfusion exercise group than in purely ischemic reperfusion group. The difference between two groups has extremely statistical significance.
3. In premature and low birth weight high risk infants group by early synthetic intervention, It’s MDI score 1.6 high than routine group in 6 months. There is no significant difference between two groups; Intervention group score 2.2, 7.6 and 7.7 higher than routine group during 12, 18 and 24 month age; the difference have extremely statistical significance. For PDI contrast, intervention group score 3.8, 9.1 12.2 and 12.6 higher respectively than routine group during 6, 12 18 and 24 months age. All months age compared have extremely statistical significance.(p<0.01)
4. Compare early exercise intervention HIE group with control group, check their MDI and PDI in 6 and 12 months age, the results shows MDI has no significance difference in 6 months age but PDI do has. In 12 months age , both MDI and PDI in intervention group much higher than control group. The difference have extremely statistical significance.(P<0.01)
Conclusion:
1. Early exercise training can promote limbs function recovery for minor MCAO rats, re-regulation MAP-2 express at ischemic cortical area in brain tissue. It suggest that rat limbs function recovery maybe related to nerve regeneration in molecular level.
2. Early synthetic movement intervention can obviously improve the prognosis of brain injuried high risk neonates and HIE patients, promote nerve regeneration and recovery of brain function, cut down nervous system sequel, thus elevate life quality for brain injury neonates.
3. Early synthetic movement intervention can obviously improve mental development for premature and low birth weight neonates. By enrichment training of earlier start moving and motor capacity, can obviously improve motor development and enhance mental development for premature and low birth weight neonates.
4. Early exercise intervention has a certain effect. It is worth spreading and utilizing in the basic level child care department and community by family connective clinic service intervention mode, and provide supports for elevation population diathesis.
引文
1.张家骧,魏克伦,薛辛东主编.新生儿急救学.北京人民卫生出版社. 2000.11.
3. Longa EZ ,Weinstein PR ,Carlson S ,et al. Reversible middle cerebral artery occlusion without craniectomy in rats. Stroke, 1989,20 :84-91.
4.高谦,吴宗耀,姚志彬,等。运动训练促进小鼠局灶脑缺血后功能恢复。中华物理医学与康复杂志,2000 ,22 :273-275。
5. Lin RCS, Matesic DFM. Immunohistochemical demonstration of neuron-specific enolase and microtubule-associated protein 2 in reactive astrocytes after injury in the adult forebrain. Neuroscience, 1994,60:11-16
6. GILLAND E. BONA E. HAGBERC H. Temporal changes of regional glucose use. Blood flow. And microtubule-assoeiated protein 2 immunostaining after liy poxia-ischemia in the immature rat brain [J]. J Cereb Blood Flow, Metab, 1998.18(2):222-228
7. Longa EZ, Weinstein PR, Carlson S, et al. Reversiblemiddle cereral artery occlusion without craniectomy in rats. Stroke. 1989,20 1 :84.
8. Jin K, MinamiM, Lan JQ, et al. Neurogenesis in dentate subgranu2 lar zone and rostral subventricular zone after focal cerebral ischemia in the rat. ProcNatlAcad SciUSA. 2001,98 8 :4710.
9.Walter E, Kaufmann, Shawn M, et al. Dendmtic Cytoskeletal Protein Expression in Mental Retardation: An Immunohistochemical Study of the Neocortex in Rett Syndrome. Cerebral Cortex, 2000,10(10),992-1004
10.Pettiew L, HoltzML, Craddock SD, et al. Microtubular proteolysis in focal cerebral ischemia. J Cereb Blood Flow Metab. 1996, 16 6 : 1189.
11.ZHAO J,MA Y, QI J. Expression of Cytoskeleton and Apoptosis Related Genes after Cerebral Infarction. Neurol Res. 2006, 28(1):71.
12.LI Y, JIANG N , POWERS C, et al. Neuronal Damage and Plasticity Identified by Microtubule-associated Protein 2, Growth-associated Protein 43, and Cyclin D1 Immunoreactivity after Focal Cerebral Ischemia in Rats. Stroke, 1998, 29(9):1972.
13.Kosik KS ,Drecchio LD ,Bakalis S ,et al. Partial sequence of MAP-2 in the region of a shared epitope with Alzheimer neurofibrillary tangles. J Neurochem , 1988, 51 :587-598.
14.SHAFTT-ZAGARDO B, ROCKWOOD J, DAVIES P, et al. Novel microtubule-associated protein-2 isoform is expressed early in human oligodendrocyte maturation [J]. Glia,2000,29(3):233-245
15.余琳,陈敦金,叶政.宫内缺血缺氧性脑损伤新生大鼠脑组织中微管相关蛋白2 mRNA的表达[J].实用医学杂志,2006,22(18):2099-2101
16.钱军,孙正义,马延超,等.大鼠脊髓损伤后MAP-2的表达及与运动功能恢复的相关性[J].西安交通大学学报,2006,27(5):489-492
17.高谦,姚志彬,袁群芳,等.缺血前运动训练对小鼠脑局灶缺血后功能恢复的影响[J].中国康复医学杂志,1999,14(3):109-111
1金汉珍,黄德珉,官希吉,等.实用新生儿学[M]。第二版,北京:人民卫生出版社,1996.68-72
2刘湘云,林传家,薛沁冰,等。实用新生儿学[M] .第一版,南京:江苏科学技术1996.339-342
3 Inage yw ,Itoh M ,Takeshima S. Correlation between cerebrovascular maturity and periventricular leukomalacia[J]. Pediatr Neurol ,2000,22(3):204-208
4李松,洪世欣,王大梅,等。早产儿和低出生体重及小于胎龄儿与脑性瘫痪发生的关系[J],中华儿科杂志,2003,41:334-347
5鲍秀兰.新生儿行为和0~3岁教育[M].北京:中国少年儿童出版社, 1995,318-327
6 CLAUDINE AT,ALBERT GRENIER .Neurological assessment during the first year of life. New York::Oxford University press,1986:46-95,182-191
7李树春主编.小儿脑性瘫痪[M].郑州:河南科学技术出版社,2000,10:209-229
8范存仁.婴幼儿智能发育测验手册.北京:团结出版社,1988,38-164
9.侯新琳,周丛乐.早产儿的脑发育问题及其检查与评价[J].中华围产医学杂志,2005,8(1):61.
10.江克文.高危儿雨后的前瞻性研究及早期预报[J].中华儿科杂志, 2001,39(1):51
11.Perlman JM. Neurobehavioral deficits in premature graduates of intensive care-potential and neonatal environmental risk factors[J]. Pediatrics,2001, 108(6): 1339-1348.
12.Wagner BP, Nedelcu J, Martin E. Delayed postischemic hypothermia improves long term behavioral outcome after cerebral hypoxia ischemia in neonatal rats [J]. Pediatr Res, 2002,51(3):354-356.
13.Tan S, Parks DA. Preserving rain function during neonatal asphyxia[J]. Clin perinatol, 1999.26(3):733-747.
14.Pamey CT, Ramey SI Early intervention and early experiencel[J]. American Pychologist 1998,53:109-120.
15.鲍秀兰.促进智力发育、预防智力低下,重在早期教育[J].中国儿童保健杂志, 2001,9(1):32-34.
16.Ramey CT, Ramey ST. Prevention of intellectual disabilities:early interventions to improve cognitive development . Prev Med .1998 Mar–Apr:27(2):224-232
17.王小兰,朱静秋,纳志云,等.昆明地区早产儿早期干预研究[J] .中国儿童保健杂志,2004,12(2):159-160
18.顾丽,黄纳新,刘培斌,等.早期干预对高危新生儿智能发育的影响[J].郧阳医学院学报,2006,25(3):161.
19.杜开先,贾天明,栾斌,等.早期干预对新生儿脑损伤神经心理发育的影响[J].中国实用神经疾病杂志,2007,10(5):5-7.
20.胡素君,蔡春玲,王玲.早期干预对高危新生儿智能及运动发育的影响[J].中国实用神经疾病杂志,2008,11(2):86-88.
21.沈晓明.儿童脑科学研究的临床和教育意义:从研究到应用[J].中国儿童保健杂志,2006,14(1):1
22.Ment LR, Vohr B, Allan W, et al . Change in cognitive function over time in very low birth weight infant . JAMA 2003 Feb 12, 289(6):705
23.Muller F, Kunesch E , Binkofski F ,et al . Residual sensorimotor functions in a patient after right–side hemispherectomy [J] .Neuropsychologia , 1991,29: 125-145
1中华医学会儿科分会新生儿学组.新生儿缺氧缺血性脑病诊断依据与临床分度[J].中华儿科杂志,1997,35(2):99~100
2鲍秀兰.新生儿行为和0~3岁教育[M].北京:中国少年儿童出版社,1995:240-276
3 Scrutton D, Damianl D, Mayston M. Management of the motor disorders of children with cerebral palsy[M]. U.S.A: Mac Keith Press,2004. 151-152
4鲍秀兰.新生儿行为和0~3岁教育[M].第2版.北京:中国少儿出版社,1996,318~327
5范存仁.婴幼儿智能发育测验手册.北京:团结出版社,1988,38~164
6周丛乐.新生儿缺氧缺血性脑病时脑细胞能量代谢过程的检测[J].中国实用儿科杂志,2000,330~331
7曹云.未成熟脑的可塑性与高危新生儿早期干预[J].中国当代儿科杂志,2003,5(6):574
8金汉珍,黄德珉,官希吉主编.实用新生儿学[M].第3版,北京:人民卫生出版社,2004,762
9董力杰.早期干预促进患儿智能发育临床研究[J].临床儿科杂志,2004,22(9):619
10楚春玲,常跃玲,孙金营,等.早期干预对新生儿缺氧缺血性脑病神经行为预后的影响[J].实用全科医学,2007,5(1):25
11董萍.早期干预对新生儿缺氧缺血性脑病预后影响的效果评价[J].中国优生与遗传杂志,2007,15(12):82
12李勇,程锐,许植之,等.早期干预对新生儿缺氧缺血性脑病预后的影响[J].临床儿科杂志,2006,24(3):188-190
13 Wynder EL. Introduction to the report on the conference on the“critical”period of brain development. Prev Med. 1998,37:166~167
14 Ramey CT, Ramey ST. Prevention of intellectusl disabilities early interventions to improve cognitive development[J]. Prev Med, 1998,27(20):224
15 Mark S. Neurophysiological assessment of brain function and maturation: A measure of brain adaptation in high risk infants[J]. Pediatr Neurol, 1997,16 (3):193
16黄水清,黄晓睿.婴儿抚触对促进婴儿生长发育的作用.中国妇幼保健,2000,15:568~569
17 Field TM . Interventions for premature infants. J Pediatr 1986,109:183~191
18南登昆.康复医学[M] .第二版.北京:人民卫生出版社,2001,121
19 Field T. Grizzle N, Scafid F, ea al. Massage and relaxation the rapies effects on depressed adolescent mothers. Adolescence , 1996,31:903~911
1. Wiche G, high M. Microtuble-associated proteins proertics and functions Biochem. 1989,2(59):1
2. Maccioni RB. Cambiazo V. Role of microtubule-associated proteins in the control of microtubule asscmbule assembly Physiol Rev 1995,75 (4):835
3. Maccioni RB,Cambiazo V. Role of microtubule-associated proteins in the control of microtubule assembly. Physl Rev, 1995,75:835-864
4. Avila J. Microtubule function. Life Sci 1991,50:327-334.
5. Longa EZ, Weinstein PR Carlson S et al. Reversible middle cereb-ratery occlusion without craniectomy in rats Stroke 1989,20(1):84
6. Ekiksson B, Arnberg H, Hellman U, et al. Chromogranins-new sensitive markers for neuroendocrine tumors. Acta Oncol 1989,28:325-329.
7. Brambklla E, Veale D, Moro D, et al. Neuroendocrine phenotype in lung cancers. Am J Clin pathol 1992,98:88-97.
8. Zhang PJ, Harris KS, Alobeid B, Brooks JJ. Immunoexpression of villin in neuroendocrine tumors and its diagnostic implications. Arch Pathol Lab Med 1999,123:812-816.
9. Gould VE, Lee I, Wiedenmann B, et al. Synaptophysin: a novel marker for neurons, certain
10. Addis B, Hamid Q, Ibrahim NB, et al. Immunohistochemical markers of small cell carcinoma and related neuroendocrine tumors of the lung. J Pathol 1987, 153:137-150.
11. Kiriakogiani-Psaropoulou P, Malamou-Mitsi V, Martinopoulou U, et al. The value of neuroendocrine markers in nonsmall cell lung cancer:a comparative immunohistopathologic study. Lung Cancer 1994,11:353-364.
12. Giordana MT, Cavalla P, Dutto A, et al. Is medulloblastoma the same tumor in children and adults? Am J Surg Pathol 1996,20:413-418.
13. Soylemezoglu F, Soffer D, Onol B, et al. Lipomatous medulloblastoma in adults. A distinct clinic opathologic entity. Am J Clin Pathol 1994,102:163-170.
14. Hessler RB, Lopes MB, Frankfurter A, et al. Cytoskeletal immunohistochemistry of central neurocytomas. Am J Surg Pathol 1992,16:1031-1038.
15. AnSJ, SeoMD,KimHS ,et al. Accurulation of microtubule-associated proteins in the hippocarpal ineuronsofseizure-sensitivegerbils [J]. Molecules and Cells,2003,15:200-207
16. Kato K, Masa T, Tawa ra Y,et al. Dendritic aberrations in the hippocampal granular layer and the amygdale ohippocampal area following kindled- scezures [J]. Brain Research 2001,901:281-295
17. Proper EA, Oestreicher AB, JansenGH ,et al. Inmunohistochemical characterization of mossy fibresprouting in the hippocampus of patients with phamaco- resistant temporal lobe epilepsy[J]. Brain ,2000, 123:19-30
18.郭珍立,柯贤军,王学峰,等.微管相关蛋白-2在难治性癫痫患者颞叶中的表达[J].中国临床神经科学. 2006,14(4):368-372
19. Johansson BB. Brain plasticity and stroke rehabilitation. The Willis Lecture[J]. Stroke,2000,31(1):223-230
20. Yi Li, Ning Jiang, Powers C, et al. Neuronal damage and plastieity identified by microtubule-associated protein 2, growth-associated protein 43, and cyelin DI immunoreactivity after focal ccrebral ischcmia in rats [J]. Stroke,1998, 29(9):1972-1980
21. Stroemer RP, Kent TA, Hulscbosch CE. Neocortical Neural sprouting, synaptogenesis and Behavioral Recovery after Neocortical infarction in Rats [J]. Stroke, 1995,26(11):2135-2144
22.高磊,王茂斌.脑的可塑性与卒中康复[J].国外医学·脑血管疾病分册,.2000,8(6):368-371
23.钱军,孙正义,马延超,等.大鼠脊髓损伤后MAP-2的表达及与运动功能恢复的相关性[J].西安交通大学学报,2006,27(5):489-492
24. Kowalski RJ, Williams RC Jr. Microtubule-associated protein 2 alters the dynamic properties of microtubule assembly and disassembly[J]. J Biol Chem,1993,268(13):9847-9855
25.高谦,姚志彬,袁群芳,等.缺血前运动训练对小鼠脑局灶缺血后功能恢复的影响[J].中国康复医学杂志,1999,14(3):109-111
26.陈运才,姚志彬,陈以慈,等.运动对小鼠小脑皮质和脊髓内突触素年龄变化的影响[J].解剖学报,1994,25(3):263-267
27.余茜,李晓红,吴士明,等.运动训练后脑缺血大鼠学习记忆与健侧脑内突触结构变化的关系[J].中华物理医学与康复杂志,2002,24(7):399-401
28. Johnson GVW, Litersky JM, Whitaker JN. Proteolysis of microtubule– associated protein 2 and tublin by cathepsin D[J].Neurochem,1991, 57:1577- 1583
29. BRIONES T L,WOODS J, WADOWSKA M, et al. Amelioration of cognitiveimpairment and changes in microtubule-associated protein 2 after transient global cerebral ischemia are influeced by complex environment experience [J]. Behav Brain, 2006,168(2):261-271
30. AN S J, SEE M O, KLM H S, et al. Accumulation of microtubule-associated proteins in the hippocampal neurons of seizure-sensitive gerbils [J]. Mol Cells, 2003,15(2):200-207
31. BUDDLE M, EBERHARDT E, CLLMNELLO L H, et al. Microtubule-associated protein 2(MAP-2) associates with the NMDA receptor and is spatially redistributed within rat hippocampal neurons after oxygen-glucose deprivation [J]. Brain Res, 2003,978(1/2):38-50
32. Lin RCS, Matesic DFM. Immunohistochemical demonstration of neuron-specific enolase and microtubule-associated protein 2 in reactive astrocytes after injury in the adult forebrain. Neuroscience, 1994,60:11-16
33. GILLAND E. BONA E. HAGBERC H. Temporal changes of regional glucose use. Blood flow. And microtubule-associated protein 2 immunostaining after hy poxia-ischemic in the immature rat brain [J]. J Cereb Blood Flow, Metab, 1998.18(2):222-228
34. Folkerts MM, Beman RE, Muizerlaar TP, et al. Disruption of MAP-2 immunostaining in rat hippocampus after traumatic in injury Neurotrauma, 1998,15(5):349
35. SHAFTT-ZAGARDO B, ROCKWOOD J, DAVIES P, et al. Novel microtubule- associated protein-2 isoform is expressed early in human oligodendrocyte maturation [J]. Glia,2000,29(3):233-245
36.余琳,陈敦金,叶政.宫内缺血缺氧性脑损伤新生大鼠脑组织中微管相关蛋白2 mRNA的表达[J].实用医学杂志,2006,22(18):2099-2101 .
1 .鲍秀兰.新生儿行为和0~3岁教育[M] .北京:中国少年儿童出版社,1995,318-327.
2.Stanley FJ, Blair E.Cerebral palsy [A].In:Pless B, ed.The epidemiology of childhood disorders[M].London:Oxford University Press,1994.473-497.
3.李松,洪世欣,王大梅,等.早产儿和低出生体重及小儿胎龄儿与脑性瘫痪发生的关系[J] .中华儿科杂志,2003,41:344-347.
4.何守森,李桂英,邢光红,等.早产儿与足月儿早期促进模式及其效果[J] .中国儿童保健杂志,2005,13(5):385-389.
5.Alfred LS.Diagnostic approach to the infant [A]In :Alfred L Scherzer ,ed. Early Diagnosis and Interventional Therapy in Cerebral Palsy[M] 3rd ed.New York :Marcel Dekker Inc , 2001.49-94.
6.沈晓明,金星明,等.发育与行为儿科学[M] .南京:江苏科学出版社,2003.451-454.
7. Perlman JM . Neurobehavioral deficits in premature graduates of intensive care-potential medical and neonatal environmental risk factors. pediatrics, 2001,108(6):1339-1348.
8.樊利春,张静.早期教育对儿童心理发育的影响.中国妇幼保健[J] .2005. 20(6):767-769.
9.金汉珍,黄德岷,官希吉,等.实用新生儿学[M] .第二版,北京:人民卫生出版社,1996.68-72.
10.刘湘云,林传家,薛沁冰,.等[M.]第二版,南京:江苏科学技术出版社,1996.339-342.
11.庞万良,翁淑萍,杨长仪.早产儿脑室周围白质软化20例B超表现及分析[J] .中国实用儿科杂志,2005,20(2):78-80.
12.Inage YW ,Itoh M ,Takashima S Correlation between cerebrovascular maturity and periventricular leukomalacia[J] Pediatr Neurol,2000,22(3) 204-208.
13.Yothy A,Raogn, Kumar KS, et al Translocation Down Syndrom[J] Indian Medsci, 2002,56 225-229.
14. Daily DK, Ardinger HH,Holmes GE Identification and evalution of mental retardation[J] Am Fam Physican,2000,61(4) 1062-1064.
15.李燕春,脑性瘫痪的致病因素及预防[J]中国康复理论与实践, 2003,9(4):2004.
16.徐海青,张蕴诚,辛玉霞,等早期生物和环境因素对早产儿智力发育的影响研究[J]中国儿童保健杂志,1997,5(3):157-158.
17.Cloonan HA, Maxwell SR, Miller SD Developmental outcomes in very low birth weight infants a six-year study[J] W V Med J 2001 Sep-Oct 97(5): 250-2.
18.Ramey CT, Ramey SL Prevention of intellectual disabilities early interventions to improve cognitive development Prev Med 1998 Mar-Apr 27(2) :224-32.
19.Blair C, Ramey CT, Hardin JM Early intervention for low birthweight,premature infants participation and intellectual development Am J Ment Retard 1995 Mar;99(5):542-54.
20.周晓玉,赵卫华,许植之,等,早期干预对早产儿预后的影响[J]临床儿科杂志,2005,23(5):297-299.
21.Thomposon RA Development in the first years of life[J] . Future Child, 2001 11(1):22.
22.鲍秀兰,孙淑英,虞人杰,等塑造最佳的人生开端[M]北京中国商业出版社,2002 193-196.
23.童雪涛,董海燕,李爱荣,等临床、保健、康复相结合的脑性瘫痪早期干预模式[J]中国康复理论与实践2003 9(5):276-277.
24.赵萍,邹小兵,周春梅,等高危儿行为与发育的早期干预治疗[J]中国儿童保健杂志,2004, 12(3):204-208.
25.Blair C Early intervention for low birth weight, preterm infants the role of negative emotionality in the specification of effects Dev Psychopathol 2002 Spring 14(2):311.
26.鲍秀兰,王丹华,孙淑英,等早期干预降低早产儿脑瘫发生率的研究[J]中国儿童保健杂志,2006,14(1):42-45.
27.Boocock SS Early Childhood programs in other nations goal and outcomes Future Child 1995 Winter 5(3):94.
28.Ment LR Vohr B,Allan W, et al Change in cognitive function over time in very low birth weight infant JAMA 2003 Feb 12 289(6) :705.
29.鲍秀兰,早期干预促进围产高危儿智能发育[J]中国临床医生,1999,27(12):3.
30.顾丽,黄纳新,刘培斌,等.早期干预对高危新生儿智能发育的影响[J].郧阳医学院学报,2006,25(3):161.
31.杜开先,贾天明,栾斌,等.早期干预对新生儿脑损伤神经心理发育的影响[J].中国实用神经疾病杂志,2007,10(5):5-7.
32.胡素君,蔡春玲,王玲.早期干预对高危新生儿智能及运动发育的影响[J].中国实用神经疾病杂志,2008,11(2):86-88.
33. A.nderson LM,Shinn C Fullilove MT et al The effectiveness of early childhood development programs A systematic review Am J Prev Med 2003 Apr 24(3 suppl) 32.
34.Reynolds AJ, Temple JA, Robertson DL, et al Long-term effects of an early childhood intervention on educational achievement and juvenile arrest A 15-year follow-up of low-income children in pubic schools JAMA2001 May 9 285(18) 2339.
35.Yoshikawa H Long-term effects of an early child programs on social outcomes and delinquency Future Child 1995 Winter 5(3) 51.
36.Bamett SW Lives in the balance age 27 benefit-cost analysis of the High/Scope Perry Preschool Program[J] Y psilanti,MI,High/Scope Press(No.1056),1996.
37.Foorman BR, Anthony J,Seals L,Mouzaki A. Language development and emergent literacy in preschool. Semin Pediatr Neurol.2002 Sep;9(3) 173.
38.Baker A J L, Piontrkowski C S, Brooks G J The effects of the Home Instrction program and one year later[J].Early Childhood Research Quarterly, 1998,13(10) 571.
39.Harr J,Higgins S,Russell P.Characteristics of families enrolled in an isolated, mountain Head Start program:managing social capital. Issues Compr Pediatr Nurs 2001 Apr~Jun;24(2) 117.
40.Stormshak EA, Kaminski RA,Goodman MR. Enhancing the parenting skills of Head Start familes during the transition to kindergarten.Prev Sci. 2002,Sep;3(3) 223.
41 . Poresky RH, Daniels AM, Two-year comparison of income,education,and depression among parents participating in regular Head Start or supplementary Family Service Center Services,Psychol Rep.2001 Jun;88(3Pt 1) 787.
42.New M,Razzino B, Lewin A,Schlumpf K,Joseph J,Mental health service use in a community head start population.Arch Pediatr Adolesc Med.2002 Jul;156(7) 721.
43.Vargas CM,Monajimy N,Khurana P,Tinanoff N. Oral health status of preschool children attending Head Start in Maryland,2000.Pediatr Dent. 2002 May~Jun; 24(3) 257.
44.Gable S,Lutz S,Nutriong socialization experiences of children in the Head Start program. J Am Diet Assoc.2001 May;101(5):572.
45 . Beaulieu E,Dufour L A,Beaudet R.Better oral health for infants and toddlers:acommunity based program. J Dent Hyg.2000 Spring;74(2):131.
46.Reid M J. Webster~Stratton C, Beauchaine T P. Parent training in head start:a comparison of program response among African American ,Asian American, Caucasian,and Hispanic mothers. Prev Sci. 2001,Dec;2(4):209.
47.郝波儿童早期心理发展干预国外医学妇幼保健分册2001 13(5):233.
48. Webster~Stratton C, Reid M J ,Hammond M. Preventing conduct problems, promoting social competence:a parent and teacher training partnership in head start, J Clin Child Psychol, 2001 Sep;30(3):283.
49.温天莲,朱薇薇,彭惠早期干预对早产儿婴儿期智能发育的影响[J]中国儿童保健杂志,2005,13(2):114-116.
⒈Premer,Ragu Rao.HIE(http://www.peds.umn.edu/divisions/neonatology/tfiles/ hie.htm1)1999.
⒉Korst LM,Phelan TP. Acute fetal asphyxia and permanent brain injury a retro- spective ana- lysis of current indicators[J].J Materm Fetal Med,1999,8:101-106
⒊William H, Ballard RA , et al . Avery′S diseases of the Newborn [M].7ed.Philadelphia:Saunders,1999.855-858.
⒋中华医学会儿科学分会新生儿学组.新生儿缺氧缺血性脑病临床诊断依据和分度[J].中华儿科杂志,1990,28:31.
⒌中华医学会儿科学分会新生儿学组.新生儿缺氧缺血性脑病诊断依据和临床分度[J].中华儿科杂志,1997,35:99.
⒍中华医学会儿科分会新生儿学组.新生儿缺氧缺血性脑病诊断标准[J].中国当代儿科杂志,2005,7(2):97-98.
⒎刘登礼,汪吉梅,邵肖梅.振幅整合脑电图在新生儿缺氧缺血性脑病中的应用价值[J].临床儿科杂志,2005,23(5):324-325.
⒏Volpe JJ.Neurology of the Newborn[M].4ed.Philadelphia: Saunders,2001.338
⒐Mary AR.MRI of the neonatal brain[J].London:Harcourt Publishers,2002,148
⒑Rutherford MA,Permock JM,Counsell sJ,et a1.Abnormal magnetic resonance signal in the internal capsule predicts poor neuredevelopmental outcome in infants with hypoxie—ischemic encephalopathy[J].Pediatrics,1998,102(2):323
11. Krishnamoorthy KS,Soman TB,Takeoka M,et a1. Difusion- weighted imaging in neonatal cerebral infarction :clinical utility and follow-up[ J]. Child Neurol,2000,15(9):592
12.Wolf RL,Zimmerman RA,Claney R,et a1.Quantitative apparent diffusion coefficient measurements in term neonates for early detection of hypoxic—ischemic brain injury:initial experience[J].Radiology,2001,218(3):825
13. Soul JS , Robertson RL , Tzika AA , et a1 . Time course of changes in diffusion-weighted magnetic resonance imaging in a case of neonatal encephalopathy with defined onset and duration of hypoxic-ischemie insult [J].Pediatrics,2001,108(5):1211
14.Selakovic V. Neuron-specific enolase in cerebrospinal fluidand plasma of patients with acute ischemic brain disease[J] .Med Pregl, 2003, 56 (7-8) :326-332 .
15.虞人杰.新生儿缺氧缺血性脑病诊断与治疗的现状与新动向[J].中华儿科杂志, 2005, 43 (8):561-563.
16.虞人杰,曹连义.新生儿颅内病变的CT诊断[J].中国实用儿科杂志, 2005,20:65-67.
17.Corff KE,McCann DL.Room air resuscitation versus oxygen resuscitation in the delivery room [J].J Perinat Neonatal Nuts,2005, l9(4):379-390.
18. Ramji S , Rasaily R , Mishra PK , Narang A , Jayam S , Kapoor AN ,et a1.Resuscitation of asphyxiated newborn with room air or 100% oxygen at birth:a mulficenter trial[J].Indian Pediatr, 2003,40(6):510-517.
19. Tan A,Schulze A,O’Donnell CPF,Davis PG.Air versus oxygen for resuscitation of infants at birth[J ]1.Coohrane Database Syst Rev,2005,18(2):CD002273.
20.Davis PG,Tan A,O’Donnell CPF.Schulu A.Resuscitation of newborn infants with 1O0% oxygen or air :a systemic review and metaanalysis[J].Lancet,2004, 364(9442):1329-1333.
21.Saugstad OD,Ramji S,Irani SF,E1-Meneza S,Hernandez EA, Vento M .et al. Resuscitation of newborn infants with 21% or 100% oxygen:follow-up at l8 to 24 months[J].Pediatrics, 2003,112(2):296-300.
22.Vento M,Asensi M,Sastre Lloret A,Garcia-Sala F,Vina J.Oxidative stress in asphyxiated term infants resuscit-ated with 100% oxygen[J].J Pediatr,2003,142(3):240-246.
23.Munkeby BH,Borke WB,Bjomland K,Sikkeland LI,Borge GI, Halvorsen B,et a1. Re- suscitation with 100% O2 increases cerebral injury in hypoxemic piglets[J].Pediatr Res 2004,56(5):783-790.
24. Behrman RE,Kliegman RM,Jenson HB,et al.Nelson Textbook of Pediatrics[M]. 17ed. Philadelphia:Saunders ,2004.566-568.
25.Perlman JM . Intervention strategies for neonatal hypoxic-ischemic cerebral injury[J].Clin Ther,2006,28(9):1353-1365.
26.Shalak LF,Laptook AR,Velaphi SC, Perlman JM.Amplitude-integrated elect roencephalo- graphy coupled with an early neurologic examination enhances prediction of term infants at risk for persistent encephalopathy[J].Pediatrics,2003,111(2):351-357
27. Hall RT,Hall FK,Daily DK.High dose phenobarbital therapy in term newborninfan- ts with severe perinatal asphyx ia:A randomized.prospective study with three-year follow-up[J].J Pediatr,1998,132(2):345-348.
28.Volpe JJ.Neurology of the newborn [M]. 4th. Philadelphia : Saunders, 2001. 217- 276,298-382.
29.王来栓,于立君,邵肖梅.亚低温减轻新生大鼠缺氧缺血脑细胞凋亡的作用及机制研究[J].中国当代儿科杂志,2007,9(1):37-41.
30.邵肖梅.新生儿缺氧缺血性脑病的亚低温治疗[J]..中国实用儿科杂志,2006,21(9):647-650.
31. Perlman JM.Brain injury in the term infant[J].Semin Perinatol,2004,28:415-424.
32.Gluckman PD,Wyatt JS,Azzaparat D,Ballard R,Edwards AD,Ferfiero DM,et a1 . Selective head . cooling mild systemic hypothermia after neonata encephalopathy: multicentre randomized trial[J].1ancet,2005,365(9460):663-670.
33.Shankaran S,Laptok AR,Ehrenkranz RA,Tyson JE,McDonald SA,Donovan EF, et a1. Whole—body hypotherm ia for neonates with hypoxic—ischemic encephalopathy[J].N Earl J Med,2005,353(15):1574—1584.
34.选择性头部亚低温治疗新生儿HIE多中心协作组.亚低温治疗新生儿缺氧缺血性脑病临床床多中心研究阶段性疗效分析[J].中国循证儿科杂志,2006,2(1):99—105.
35.刘玲,杨于嘉,文秋生,王鸿娟,王多德.高压氧治疗对新生大鼠缺氧缺血性脑损伤模型的保护作用[J].实用儿科临床杂志,2005,20(6):531-532. .
36.史源,李华强.高压氧治疗新生儿缺氧缺血性脑病国内外研究进展[J].实用儿科临床杂志,2006,21(14):951-952.
37.Liu ZL,Xiong TB,Meads C.Clinical efectiveness of treatment with hyperbaric oxygen for neonatal hypoxic-ischaemic encephalopathy:systematic review of Chinese literature[J].BMJ,2006,333(7564 ):374.
38.Pineus DW ,Goodmsn RR,Fraser RA,et a1. Neural stem and progenitor cell:a strategy for gene therapy and brain repai[J]r.Neurosrug,1998,42:858-867.
39.Kim HT,Kim IS,Lim SE,Lee IS,Park KI.Gene and cell replacement via neural stem cells [J].Yonsei Med J,2004,45(Supp1):32-40.
40.Satoshi OS , James EG . Multiple cell populations in the early postnatal subventricular zone take distinct migratory pathways: a dynamic study of glial and neuronal progenitor migration [J].J Neurosci,2003,23:4240-4250.
41.栾佐,尹国才,胡晓红,屈素清,吴南海,严凤清,等.人神经干细胞移植治疗重度新生儿缺氧缺血性脑病一例[J].中华儿科杂志,2005,43(8):580-583.
42.新生儿缺氧缺血性脑病治疗协作组.新生儿缺氧缺血性脑病第二次多中心治疗总结[J].中国实用儿科杂志,2003,18(9):558-560.
43.Mark S.Neurophysiological assessment of brain function and maturation : a measure of brain adaptation in high risk infant[J]. Pediatri Neurol, 1997,16(3):193-198.
44.Eken P,Gronendatul F,Kademaker K,et al.Early assessment of the term infant whit HIE[J].Dev Med child,1995,37 (3):72-74
45.李勇,程锐,许植之,等.早期干预对新生儿缺氧缺血性脑病预后的影响[ J].临床儿科杂志,2006,24(3):188-190
46. Vento M,Asensi M,Sastre Lloret A, Garcia-Sala F,Vina J.Oxidative stress in asphyxiated term infants resuscitated with 100% oxygen[J].J Pediatr,2003,142(3):240-246.
47. Shalak L,Perlman JM.Hypoxic-ischemic brain injury in the term infant-current concepts[J].Early Hum Dev, 2004,80(20):125-141.
48.Ferriero DM.Timing is everything-delaying therapy for delayed cell Death[J],Dev Neurosci,2002,24(5):349-351.
3. Longa EZ ,Weinstein PR ,Carlson S ,et al. Reversible middle cerebral artery occlusion without craniectomy in rats. Stroke, 1989,20 :84-91.
4.高谦,吴宗耀,姚志彬,等。运动训练促进小鼠局灶脑缺血后功能恢复。中华物理医学与康复杂志,2000 ,22 :273-275。
5. Lin RCS, Matesic DFM. Immunohistochemical demonstration of neuron-specific enolase and microtubule-associated protein 2 in reactive astrocytes after injury in the adult forebrain. Neuroscience, 1994,60:11-16
6. GILLAND E. BONA E. HAGBERC H. Temporal changes of regional glucose use. Blood flow. And microtubule-assoeiated protein 2 immunostaining after liy poxia-ischemia in the immature rat brain [J]. J Cereb Blood Flow, Metab, 1998.18(2):222-228
7. Longa EZ, Weinstein PR, Carlson S, et al. Reversiblemiddle cereral artery occlusion without craniectomy in rats. Stroke. 1989,20 1 :84.
8. Jin K, MinamiM, Lan JQ, et al. Neurogenesis in dentate subgranu2 lar zone and rostral subventricular zone after focal cerebral ischemia in the rat. ProcNatlAcad SciUSA. 2001,98 8 :4710.
9.Walter E, Kaufmann, Shawn M, et al. Dendmtic Cytoskeletal Protein Expression in Mental Retardation: An Immunohistochemical Study of the Neocortex in Rett Syndrome. Cerebral Cortex, 2000,10(10),992-1004
10.Pettiew L, HoltzML, Craddock SD, et al. Microtubular proteolysis in focal cerebral ischemia. J Cereb Blood Flow Metab. 1996, 16 6 : 1189.
11.ZHAO J,MA Y, QI J. Expression of Cytoskeleton and Apoptosis Related Genes after Cerebral Infarction. Neurol Res. 2006, 28(1):71.
12.LI Y, JIANG N , POWERS C, et al. Neuronal Damage and Plasticity Identified by Microtubule-associated Protein 2, Growth-associated Protein 43, and Cyclin D1 Immunoreactivity after Focal Cerebral Ischemia in Rats. Stroke, 1998, 29(9):1972.
13.Kosik KS ,Drecchio LD ,Bakalis S ,et al. Partial sequence of MAP-2 in the region of a shared epitope with Alzheimer neurofibrillary tangles. J Neurochem , 1988, 51 :587-598.
14.SHAFTT-ZAGARDO B, ROCKWOOD J, DAVIES P, et al. Novel microtubule-associated protein-2 isoform is expressed early in human oligodendrocyte maturation [J]. Glia,2000,29(3):233-245
15.余琳,陈敦金,叶政.宫内缺血缺氧性脑损伤新生大鼠脑组织中微管相关蛋白2 mRNA的表达[J].实用医学杂志,2006,22(18):2099-2101
16.钱军,孙正义,马延超,等.大鼠脊髓损伤后MAP-2的表达及与运动功能恢复的相关性[J].西安交通大学学报,2006,27(5):489-492
17.高谦,姚志彬,袁群芳,等.缺血前运动训练对小鼠脑局灶缺血后功能恢复的影响[J].中国康复医学杂志,1999,14(3):109-111
1金汉珍,黄德珉,官希吉,等.实用新生儿学[M]。第二版,北京:人民卫生出版社,1996.68-72
2刘湘云,林传家,薛沁冰,等。实用新生儿学[M] .第一版,南京:江苏科学技术1996.339-342
3 Inage yw ,Itoh M ,Takeshima S. Correlation between cerebrovascular maturity and periventricular leukomalacia[J]. Pediatr Neurol ,2000,22(3):204-208
4李松,洪世欣,王大梅,等。早产儿和低出生体重及小于胎龄儿与脑性瘫痪发生的关系[J],中华儿科杂志,2003,41:334-347
5鲍秀兰.新生儿行为和0~3岁教育[M].北京:中国少年儿童出版社, 1995,318-327
6 CLAUDINE AT,ALBERT GRENIER .Neurological assessment during the first year of life. New York::Oxford University press,1986:46-95,182-191
7李树春主编.小儿脑性瘫痪[M].郑州:河南科学技术出版社,2000,10:209-229
8范存仁.婴幼儿智能发育测验手册.北京:团结出版社,1988,38-164
9.侯新琳,周丛乐.早产儿的脑发育问题及其检查与评价[J].中华围产医学杂志,2005,8(1):61.
10.江克文.高危儿雨后的前瞻性研究及早期预报[J].中华儿科杂志, 2001,39(1):51
11.Perlman JM. Neurobehavioral deficits in premature graduates of intensive care-potential and neonatal environmental risk factors[J]. Pediatrics,2001, 108(6): 1339-1348.
12.Wagner BP, Nedelcu J, Martin E. Delayed postischemic hypothermia improves long term behavioral outcome after cerebral hypoxia ischemia in neonatal rats [J]. Pediatr Res, 2002,51(3):354-356.
13.Tan S, Parks DA. Preserving rain function during neonatal asphyxia[J]. Clin perinatol, 1999.26(3):733-747.
14.Pamey CT, Ramey SI Early intervention and early experiencel[J]. American Pychologist 1998,53:109-120.
15.鲍秀兰.促进智力发育、预防智力低下,重在早期教育[J].中国儿童保健杂志, 2001,9(1):32-34.
16.Ramey CT, Ramey ST. Prevention of intellectual disabilities:early interventions to improve cognitive development . Prev Med .1998 Mar–Apr:27(2):224-232
17.王小兰,朱静秋,纳志云,等.昆明地区早产儿早期干预研究[J] .中国儿童保健杂志,2004,12(2):159-160
18.顾丽,黄纳新,刘培斌,等.早期干预对高危新生儿智能发育的影响[J].郧阳医学院学报,2006,25(3):161.
19.杜开先,贾天明,栾斌,等.早期干预对新生儿脑损伤神经心理发育的影响[J].中国实用神经疾病杂志,2007,10(5):5-7.
20.胡素君,蔡春玲,王玲.早期干预对高危新生儿智能及运动发育的影响[J].中国实用神经疾病杂志,2008,11(2):86-88.
21.沈晓明.儿童脑科学研究的临床和教育意义:从研究到应用[J].中国儿童保健杂志,2006,14(1):1
22.Ment LR, Vohr B, Allan W, et al . Change in cognitive function over time in very low birth weight infant . JAMA 2003 Feb 12, 289(6):705
23.Muller F, Kunesch E , Binkofski F ,et al . Residual sensorimotor functions in a patient after right–side hemispherectomy [J] .Neuropsychologia , 1991,29: 125-145
1中华医学会儿科分会新生儿学组.新生儿缺氧缺血性脑病诊断依据与临床分度[J].中华儿科杂志,1997,35(2):99~100
2鲍秀兰.新生儿行为和0~3岁教育[M].北京:中国少年儿童出版社,1995:240-276
3 Scrutton D, Damianl D, Mayston M. Management of the motor disorders of children with cerebral palsy[M]. U.S.A: Mac Keith Press,2004. 151-152
4鲍秀兰.新生儿行为和0~3岁教育[M].第2版.北京:中国少儿出版社,1996,318~327
5范存仁.婴幼儿智能发育测验手册.北京:团结出版社,1988,38~164
6周丛乐.新生儿缺氧缺血性脑病时脑细胞能量代谢过程的检测[J].中国实用儿科杂志,2000,330~331
7曹云.未成熟脑的可塑性与高危新生儿早期干预[J].中国当代儿科杂志,2003,5(6):574
8金汉珍,黄德珉,官希吉主编.实用新生儿学[M].第3版,北京:人民卫生出版社,2004,762
9董力杰.早期干预促进患儿智能发育临床研究[J].临床儿科杂志,2004,22(9):619
10楚春玲,常跃玲,孙金营,等.早期干预对新生儿缺氧缺血性脑病神经行为预后的影响[J].实用全科医学,2007,5(1):25
11董萍.早期干预对新生儿缺氧缺血性脑病预后影响的效果评价[J].中国优生与遗传杂志,2007,15(12):82
12李勇,程锐,许植之,等.早期干预对新生儿缺氧缺血性脑病预后的影响[J].临床儿科杂志,2006,24(3):188-190
13 Wynder EL. Introduction to the report on the conference on the“critical”period of brain development. Prev Med. 1998,37:166~167
14 Ramey CT, Ramey ST. Prevention of intellectusl disabilities early interventions to improve cognitive development[J]. Prev Med, 1998,27(20):224
15 Mark S. Neurophysiological assessment of brain function and maturation: A measure of brain adaptation in high risk infants[J]. Pediatr Neurol, 1997,16 (3):193
16黄水清,黄晓睿.婴儿抚触对促进婴儿生长发育的作用.中国妇幼保健,2000,15:568~569
17 Field TM . Interventions for premature infants. J Pediatr 1986,109:183~191
18南登昆.康复医学[M] .第二版.北京:人民卫生出版社,2001,121
19 Field T. Grizzle N, Scafid F, ea al. Massage and relaxation the rapies effects on depressed adolescent mothers. Adolescence , 1996,31:903~911
1. Wiche G, high M. Microtuble-associated proteins proertics and functions Biochem. 1989,2(59):1
2. Maccioni RB. Cambiazo V. Role of microtubule-associated proteins in the control of microtubule asscmbule assembly Physiol Rev 1995,75 (4):835
3. Maccioni RB,Cambiazo V. Role of microtubule-associated proteins in the control of microtubule assembly. Physl Rev, 1995,75:835-864
4. Avila J. Microtubule function. Life Sci 1991,50:327-334.
5. Longa EZ, Weinstein PR Carlson S et al. Reversible middle cereb-ratery occlusion without craniectomy in rats Stroke 1989,20(1):84
6. Ekiksson B, Arnberg H, Hellman U, et al. Chromogranins-new sensitive markers for neuroendocrine tumors. Acta Oncol 1989,28:325-329.
7. Brambklla E, Veale D, Moro D, et al. Neuroendocrine phenotype in lung cancers. Am J Clin pathol 1992,98:88-97.
8. Zhang PJ, Harris KS, Alobeid B, Brooks JJ. Immunoexpression of villin in neuroendocrine tumors and its diagnostic implications. Arch Pathol Lab Med 1999,123:812-816.
9. Gould VE, Lee I, Wiedenmann B, et al. Synaptophysin: a novel marker for neurons, certain
10. Addis B, Hamid Q, Ibrahim NB, et al. Immunohistochemical markers of small cell carcinoma and related neuroendocrine tumors of the lung. J Pathol 1987, 153:137-150.
11. Kiriakogiani-Psaropoulou P, Malamou-Mitsi V, Martinopoulou U, et al. The value of neuroendocrine markers in nonsmall cell lung cancer:a comparative immunohistopathologic study. Lung Cancer 1994,11:353-364.
12. Giordana MT, Cavalla P, Dutto A, et al. Is medulloblastoma the same tumor in children and adults? Am J Surg Pathol 1996,20:413-418.
13. Soylemezoglu F, Soffer D, Onol B, et al. Lipomatous medulloblastoma in adults. A distinct clinic opathologic entity. Am J Clin Pathol 1994,102:163-170.
14. Hessler RB, Lopes MB, Frankfurter A, et al. Cytoskeletal immunohistochemistry of central neurocytomas. Am J Surg Pathol 1992,16:1031-1038.
15. AnSJ, SeoMD,KimHS ,et al. Accurulation of microtubule-associated proteins in the hippocarpal ineuronsofseizure-sensitivegerbils [J]. Molecules and Cells,2003,15:200-207
16. Kato K, Masa T, Tawa ra Y,et al. Dendritic aberrations in the hippocampal granular layer and the amygdale ohippocampal area following kindled- scezures [J]. Brain Research 2001,901:281-295
17. Proper EA, Oestreicher AB, JansenGH ,et al. Inmunohistochemical characterization of mossy fibresprouting in the hippocampus of patients with phamaco- resistant temporal lobe epilepsy[J]. Brain ,2000, 123:19-30
18.郭珍立,柯贤军,王学峰,等.微管相关蛋白-2在难治性癫痫患者颞叶中的表达[J].中国临床神经科学. 2006,14(4):368-372
19. Johansson BB. Brain plasticity and stroke rehabilitation. The Willis Lecture[J]. Stroke,2000,31(1):223-230
20. Yi Li, Ning Jiang, Powers C, et al. Neuronal damage and plastieity identified by microtubule-associated protein 2, growth-associated protein 43, and cyelin DI immunoreactivity after focal ccrebral ischcmia in rats [J]. Stroke,1998, 29(9):1972-1980
21. Stroemer RP, Kent TA, Hulscbosch CE. Neocortical Neural sprouting, synaptogenesis and Behavioral Recovery after Neocortical infarction in Rats [J]. Stroke, 1995,26(11):2135-2144
22.高磊,王茂斌.脑的可塑性与卒中康复[J].国外医学·脑血管疾病分册,.2000,8(6):368-371
23.钱军,孙正义,马延超,等.大鼠脊髓损伤后MAP-2的表达及与运动功能恢复的相关性[J].西安交通大学学报,2006,27(5):489-492
24. Kowalski RJ, Williams RC Jr. Microtubule-associated protein 2 alters the dynamic properties of microtubule assembly and disassembly[J]. J Biol Chem,1993,268(13):9847-9855
25.高谦,姚志彬,袁群芳,等.缺血前运动训练对小鼠脑局灶缺血后功能恢复的影响[J].中国康复医学杂志,1999,14(3):109-111
26.陈运才,姚志彬,陈以慈,等.运动对小鼠小脑皮质和脊髓内突触素年龄变化的影响[J].解剖学报,1994,25(3):263-267
27.余茜,李晓红,吴士明,等.运动训练后脑缺血大鼠学习记忆与健侧脑内突触结构变化的关系[J].中华物理医学与康复杂志,2002,24(7):399-401
28. Johnson GVW, Litersky JM, Whitaker JN. Proteolysis of microtubule– associated protein 2 and tublin by cathepsin D[J].Neurochem,1991, 57:1577- 1583
29. BRIONES T L,WOODS J, WADOWSKA M, et al. Amelioration of cognitiveimpairment and changes in microtubule-associated protein 2 after transient global cerebral ischemia are influeced by complex environment experience [J]. Behav Brain, 2006,168(2):261-271
30. AN S J, SEE M O, KLM H S, et al. Accumulation of microtubule-associated proteins in the hippocampal neurons of seizure-sensitive gerbils [J]. Mol Cells, 2003,15(2):200-207
31. BUDDLE M, EBERHARDT E, CLLMNELLO L H, et al. Microtubule-associated protein 2(MAP-2) associates with the NMDA receptor and is spatially redistributed within rat hippocampal neurons after oxygen-glucose deprivation [J]. Brain Res, 2003,978(1/2):38-50
32. Lin RCS, Matesic DFM. Immunohistochemical demonstration of neuron-specific enolase and microtubule-associated protein 2 in reactive astrocytes after injury in the adult forebrain. Neuroscience, 1994,60:11-16
33. GILLAND E. BONA E. HAGBERC H. Temporal changes of regional glucose use. Blood flow. And microtubule-associated protein 2 immunostaining after hy poxia-ischemic in the immature rat brain [J]. J Cereb Blood Flow, Metab, 1998.18(2):222-228
34. Folkerts MM, Beman RE, Muizerlaar TP, et al. Disruption of MAP-2 immunostaining in rat hippocampus after traumatic in injury Neurotrauma, 1998,15(5):349
35. SHAFTT-ZAGARDO B, ROCKWOOD J, DAVIES P, et al. Novel microtubule- associated protein-2 isoform is expressed early in human oligodendrocyte maturation [J]. Glia,2000,29(3):233-245
36.余琳,陈敦金,叶政.宫内缺血缺氧性脑损伤新生大鼠脑组织中微管相关蛋白2 mRNA的表达[J].实用医学杂志,2006,22(18):2099-2101 .
1 .鲍秀兰.新生儿行为和0~3岁教育[M] .北京:中国少年儿童出版社,1995,318-327.
2.Stanley FJ, Blair E.Cerebral palsy [A].In:Pless B, ed.The epidemiology of childhood disorders[M].London:Oxford University Press,1994.473-497.
3.李松,洪世欣,王大梅,等.早产儿和低出生体重及小儿胎龄儿与脑性瘫痪发生的关系[J] .中华儿科杂志,2003,41:344-347.
4.何守森,李桂英,邢光红,等.早产儿与足月儿早期促进模式及其效果[J] .中国儿童保健杂志,2005,13(5):385-389.
5.Alfred LS.Diagnostic approach to the infant [A]In :Alfred L Scherzer ,ed. Early Diagnosis and Interventional Therapy in Cerebral Palsy[M] 3rd ed.New York :Marcel Dekker Inc , 2001.49-94.
6.沈晓明,金星明,等.发育与行为儿科学[M] .南京:江苏科学出版社,2003.451-454.
7. Perlman JM . Neurobehavioral deficits in premature graduates of intensive care-potential medical and neonatal environmental risk factors. pediatrics, 2001,108(6):1339-1348.
8.樊利春,张静.早期教育对儿童心理发育的影响.中国妇幼保健[J] .2005. 20(6):767-769.
9.金汉珍,黄德岷,官希吉,等.实用新生儿学[M] .第二版,北京:人民卫生出版社,1996.68-72.
10.刘湘云,林传家,薛沁冰,.等[M.]第二版,南京:江苏科学技术出版社,1996.339-342.
11.庞万良,翁淑萍,杨长仪.早产儿脑室周围白质软化20例B超表现及分析[J] .中国实用儿科杂志,2005,20(2):78-80.
12.Inage YW ,Itoh M ,Takashima S Correlation between cerebrovascular maturity and periventricular leukomalacia[J] Pediatr Neurol,2000,22(3) 204-208.
13.Yothy A,Raogn, Kumar KS, et al Translocation Down Syndrom[J] Indian Medsci, 2002,56 225-229.
14. Daily DK, Ardinger HH,Holmes GE Identification and evalution of mental retardation[J] Am Fam Physican,2000,61(4) 1062-1064.
15.李燕春,脑性瘫痪的致病因素及预防[J]中国康复理论与实践, 2003,9(4):2004.
16.徐海青,张蕴诚,辛玉霞,等早期生物和环境因素对早产儿智力发育的影响研究[J]中国儿童保健杂志,1997,5(3):157-158.
17.Cloonan HA, Maxwell SR, Miller SD Developmental outcomes in very low birth weight infants a six-year study[J] W V Med J 2001 Sep-Oct 97(5): 250-2.
18.Ramey CT, Ramey SL Prevention of intellectual disabilities early interventions to improve cognitive development Prev Med 1998 Mar-Apr 27(2) :224-32.
19.Blair C, Ramey CT, Hardin JM Early intervention for low birthweight,premature infants participation and intellectual development Am J Ment Retard 1995 Mar;99(5):542-54.
20.周晓玉,赵卫华,许植之,等,早期干预对早产儿预后的影响[J]临床儿科杂志,2005,23(5):297-299.
21.Thomposon RA Development in the first years of life[J] . Future Child, 2001 11(1):22.
22.鲍秀兰,孙淑英,虞人杰,等塑造最佳的人生开端[M]北京中国商业出版社,2002 193-196.
23.童雪涛,董海燕,李爱荣,等临床、保健、康复相结合的脑性瘫痪早期干预模式[J]中国康复理论与实践2003 9(5):276-277.
24.赵萍,邹小兵,周春梅,等高危儿行为与发育的早期干预治疗[J]中国儿童保健杂志,2004, 12(3):204-208.
25.Blair C Early intervention for low birth weight, preterm infants the role of negative emotionality in the specification of effects Dev Psychopathol 2002 Spring 14(2):311.
26.鲍秀兰,王丹华,孙淑英,等早期干预降低早产儿脑瘫发生率的研究[J]中国儿童保健杂志,2006,14(1):42-45.
27.Boocock SS Early Childhood programs in other nations goal and outcomes Future Child 1995 Winter 5(3):94.
28.Ment LR Vohr B,Allan W, et al Change in cognitive function over time in very low birth weight infant JAMA 2003 Feb 12 289(6) :705.
29.鲍秀兰,早期干预促进围产高危儿智能发育[J]中国临床医生,1999,27(12):3.
30.顾丽,黄纳新,刘培斌,等.早期干预对高危新生儿智能发育的影响[J].郧阳医学院学报,2006,25(3):161.
31.杜开先,贾天明,栾斌,等.早期干预对新生儿脑损伤神经心理发育的影响[J].中国实用神经疾病杂志,2007,10(5):5-7.
32.胡素君,蔡春玲,王玲.早期干预对高危新生儿智能及运动发育的影响[J].中国实用神经疾病杂志,2008,11(2):86-88.
33. A.nderson LM,Shinn C Fullilove MT et al The effectiveness of early childhood development programs A systematic review Am J Prev Med 2003 Apr 24(3 suppl) 32.
34.Reynolds AJ, Temple JA, Robertson DL, et al Long-term effects of an early childhood intervention on educational achievement and juvenile arrest A 15-year follow-up of low-income children in pubic schools JAMA2001 May 9 285(18) 2339.
35.Yoshikawa H Long-term effects of an early child programs on social outcomes and delinquency Future Child 1995 Winter 5(3) 51.
36.Bamett SW Lives in the balance age 27 benefit-cost analysis of the High/Scope Perry Preschool Program[J] Y psilanti,MI,High/Scope Press(No.1056),1996.
37.Foorman BR, Anthony J,Seals L,Mouzaki A. Language development and emergent literacy in preschool. Semin Pediatr Neurol.2002 Sep;9(3) 173.
38.Baker A J L, Piontrkowski C S, Brooks G J The effects of the Home Instrction program and one year later[J].Early Childhood Research Quarterly, 1998,13(10) 571.
39.Harr J,Higgins S,Russell P.Characteristics of families enrolled in an isolated, mountain Head Start program:managing social capital. Issues Compr Pediatr Nurs 2001 Apr~Jun;24(2) 117.
40.Stormshak EA, Kaminski RA,Goodman MR. Enhancing the parenting skills of Head Start familes during the transition to kindergarten.Prev Sci. 2002,Sep;3(3) 223.
41 . Poresky RH, Daniels AM, Two-year comparison of income,education,and depression among parents participating in regular Head Start or supplementary Family Service Center Services,Psychol Rep.2001 Jun;88(3Pt 1) 787.
42.New M,Razzino B, Lewin A,Schlumpf K,Joseph J,Mental health service use in a community head start population.Arch Pediatr Adolesc Med.2002 Jul;156(7) 721.
43.Vargas CM,Monajimy N,Khurana P,Tinanoff N. Oral health status of preschool children attending Head Start in Maryland,2000.Pediatr Dent. 2002 May~Jun; 24(3) 257.
44.Gable S,Lutz S,Nutriong socialization experiences of children in the Head Start program. J Am Diet Assoc.2001 May;101(5):572.
45 . Beaulieu E,Dufour L A,Beaudet R.Better oral health for infants and toddlers:acommunity based program. J Dent Hyg.2000 Spring;74(2):131.
46.Reid M J. Webster~Stratton C, Beauchaine T P. Parent training in head start:a comparison of program response among African American ,Asian American, Caucasian,and Hispanic mothers. Prev Sci. 2001,Dec;2(4):209.
47.郝波儿童早期心理发展干预国外医学妇幼保健分册2001 13(5):233.
48. Webster~Stratton C, Reid M J ,Hammond M. Preventing conduct problems, promoting social competence:a parent and teacher training partnership in head start, J Clin Child Psychol, 2001 Sep;30(3):283.
49.温天莲,朱薇薇,彭惠早期干预对早产儿婴儿期智能发育的影响[J]中国儿童保健杂志,2005,13(2):114-116.
⒈Premer,Ragu Rao.HIE(http://www.peds.umn.edu/divisions/neonatology/tfiles/ hie.htm1)1999.
⒉Korst LM,Phelan TP. Acute fetal asphyxia and permanent brain injury a retro- spective ana- lysis of current indicators[J].J Materm Fetal Med,1999,8:101-106
⒊William H, Ballard RA , et al . Avery′S diseases of the Newborn [M].7ed.Philadelphia:Saunders,1999.855-858.
⒋中华医学会儿科学分会新生儿学组.新生儿缺氧缺血性脑病临床诊断依据和分度[J].中华儿科杂志,1990,28:31.
⒌中华医学会儿科学分会新生儿学组.新生儿缺氧缺血性脑病诊断依据和临床分度[J].中华儿科杂志,1997,35:99.
⒍中华医学会儿科分会新生儿学组.新生儿缺氧缺血性脑病诊断标准[J].中国当代儿科杂志,2005,7(2):97-98.
⒎刘登礼,汪吉梅,邵肖梅.振幅整合脑电图在新生儿缺氧缺血性脑病中的应用价值[J].临床儿科杂志,2005,23(5):324-325.
⒏Volpe JJ.Neurology of the Newborn[M].4ed.Philadelphia: Saunders,2001.338
⒐Mary AR.MRI of the neonatal brain[J].London:Harcourt Publishers,2002,148
⒑Rutherford MA,Permock JM,Counsell sJ,et a1.Abnormal magnetic resonance signal in the internal capsule predicts poor neuredevelopmental outcome in infants with hypoxie—ischemic encephalopathy[J].Pediatrics,1998,102(2):323
11. Krishnamoorthy KS,Soman TB,Takeoka M,et a1. Difusion- weighted imaging in neonatal cerebral infarction :clinical utility and follow-up[ J]. Child Neurol,2000,15(9):592
12.Wolf RL,Zimmerman RA,Claney R,et a1.Quantitative apparent diffusion coefficient measurements in term neonates for early detection of hypoxic—ischemic brain injury:initial experience[J].Radiology,2001,218(3):825
13. Soul JS , Robertson RL , Tzika AA , et a1 . Time course of changes in diffusion-weighted magnetic resonance imaging in a case of neonatal encephalopathy with defined onset and duration of hypoxic-ischemie insult [J].Pediatrics,2001,108(5):1211
14.Selakovic V. Neuron-specific enolase in cerebrospinal fluidand plasma of patients with acute ischemic brain disease[J] .Med Pregl, 2003, 56 (7-8) :326-332 .
15.虞人杰.新生儿缺氧缺血性脑病诊断与治疗的现状与新动向[J].中华儿科杂志, 2005, 43 (8):561-563.
16.虞人杰,曹连义.新生儿颅内病变的CT诊断[J].中国实用儿科杂志, 2005,20:65-67.
17.Corff KE,McCann DL.Room air resuscitation versus oxygen resuscitation in the delivery room [J].J Perinat Neonatal Nuts,2005, l9(4):379-390.
18. Ramji S , Rasaily R , Mishra PK , Narang A , Jayam S , Kapoor AN ,et a1.Resuscitation of asphyxiated newborn with room air or 100% oxygen at birth:a mulficenter trial[J].Indian Pediatr, 2003,40(6):510-517.
19. Tan A,Schulze A,O’Donnell CPF,Davis PG.Air versus oxygen for resuscitation of infants at birth[J ]1.Coohrane Database Syst Rev,2005,18(2):CD002273.
20.Davis PG,Tan A,O’Donnell CPF.Schulu A.Resuscitation of newborn infants with 1O0% oxygen or air :a systemic review and metaanalysis[J].Lancet,2004, 364(9442):1329-1333.
21.Saugstad OD,Ramji S,Irani SF,E1-Meneza S,Hernandez EA, Vento M .et al. Resuscitation of newborn infants with 21% or 100% oxygen:follow-up at l8 to 24 months[J].Pediatrics, 2003,112(2):296-300.
22.Vento M,Asensi M,Sastre Lloret A,Garcia-Sala F,Vina J.Oxidative stress in asphyxiated term infants resuscit-ated with 100% oxygen[J].J Pediatr,2003,142(3):240-246.
23.Munkeby BH,Borke WB,Bjomland K,Sikkeland LI,Borge GI, Halvorsen B,et a1. Re- suscitation with 100% O2 increases cerebral injury in hypoxemic piglets[J].Pediatr Res 2004,56(5):783-790.
24. Behrman RE,Kliegman RM,Jenson HB,et al.Nelson Textbook of Pediatrics[M]. 17ed. Philadelphia:Saunders ,2004.566-568.
25.Perlman JM . Intervention strategies for neonatal hypoxic-ischemic cerebral injury[J].Clin Ther,2006,28(9):1353-1365.
26.Shalak LF,Laptook AR,Velaphi SC, Perlman JM.Amplitude-integrated elect roencephalo- graphy coupled with an early neurologic examination enhances prediction of term infants at risk for persistent encephalopathy[J].Pediatrics,2003,111(2):351-357
27. Hall RT,Hall FK,Daily DK.High dose phenobarbital therapy in term newborninfan- ts with severe perinatal asphyx ia:A randomized.prospective study with three-year follow-up[J].J Pediatr,1998,132(2):345-348.
28.Volpe JJ.Neurology of the newborn [M]. 4th. Philadelphia : Saunders, 2001. 217- 276,298-382.
29.王来栓,于立君,邵肖梅.亚低温减轻新生大鼠缺氧缺血脑细胞凋亡的作用及机制研究[J].中国当代儿科杂志,2007,9(1):37-41.
30.邵肖梅.新生儿缺氧缺血性脑病的亚低温治疗[J]..中国实用儿科杂志,2006,21(9):647-650.
31. Perlman JM.Brain injury in the term infant[J].Semin Perinatol,2004,28:415-424.
32.Gluckman PD,Wyatt JS,Azzaparat D,Ballard R,Edwards AD,Ferfiero DM,et a1 . Selective head . cooling mild systemic hypothermia after neonata encephalopathy: multicentre randomized trial[J].1ancet,2005,365(9460):663-670.
33.Shankaran S,Laptok AR,Ehrenkranz RA,Tyson JE,McDonald SA,Donovan EF, et a1. Whole—body hypotherm ia for neonates with hypoxic—ischemic encephalopathy[J].N Earl J Med,2005,353(15):1574—1584.
34.选择性头部亚低温治疗新生儿HIE多中心协作组.亚低温治疗新生儿缺氧缺血性脑病临床床多中心研究阶段性疗效分析[J].中国循证儿科杂志,2006,2(1):99—105.
35.刘玲,杨于嘉,文秋生,王鸿娟,王多德.高压氧治疗对新生大鼠缺氧缺血性脑损伤模型的保护作用[J].实用儿科临床杂志,2005,20(6):531-532. .
36.史源,李华强.高压氧治疗新生儿缺氧缺血性脑病国内外研究进展[J].实用儿科临床杂志,2006,21(14):951-952.
37.Liu ZL,Xiong TB,Meads C.Clinical efectiveness of treatment with hyperbaric oxygen for neonatal hypoxic-ischaemic encephalopathy:systematic review of Chinese literature[J].BMJ,2006,333(7564 ):374.
38.Pineus DW ,Goodmsn RR,Fraser RA,et a1. Neural stem and progenitor cell:a strategy for gene therapy and brain repai[J]r.Neurosrug,1998,42:858-867.
39.Kim HT,Kim IS,Lim SE,Lee IS,Park KI.Gene and cell replacement via neural stem cells [J].Yonsei Med J,2004,45(Supp1):32-40.
40.Satoshi OS , James EG . Multiple cell populations in the early postnatal subventricular zone take distinct migratory pathways: a dynamic study of glial and neuronal progenitor migration [J].J Neurosci,2003,23:4240-4250.
41.栾佐,尹国才,胡晓红,屈素清,吴南海,严凤清,等.人神经干细胞移植治疗重度新生儿缺氧缺血性脑病一例[J].中华儿科杂志,2005,43(8):580-583.
42.新生儿缺氧缺血性脑病治疗协作组.新生儿缺氧缺血性脑病第二次多中心治疗总结[J].中国实用儿科杂志,2003,18(9):558-560.
43.Mark S.Neurophysiological assessment of brain function and maturation : a measure of brain adaptation in high risk infant[J]. Pediatri Neurol, 1997,16(3):193-198.
44.Eken P,Gronendatul F,Kademaker K,et al.Early assessment of the term infant whit HIE[J].Dev Med child,1995,37 (3):72-74
45.李勇,程锐,许植之,等.早期干预对新生儿缺氧缺血性脑病预后的影响[ J].临床儿科杂志,2006,24(3):188-190
46. Vento M,Asensi M,Sastre Lloret A, Garcia-Sala F,Vina J.Oxidative stress in asphyxiated term infants resuscitated with 100% oxygen[J].J Pediatr,2003,142(3):240-246.
47. Shalak L,Perlman JM.Hypoxic-ischemic brain injury in the term infant-current concepts[J].Early Hum Dev, 2004,80(20):125-141.
48.Ferriero DM.Timing is everything-delaying therapy for delayed cell Death[J],Dev Neurosci,2002,24(5):349-351.
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