马铃薯与茄子原生质体融合创制新资源研究
|
摘要
体细胞杂交技术不仅能够克服有性生殖障碍,而且可以有效地同时转移核基因与胞质基因,促使优良性状在种内、种问、属间得到整合,从而获得有性杂交无法得到的优良种质资源,丰富现有栽培种资源的遗传背景。青枯病是茄科植物的重要的细菌性病害,其危害在马铃薯上仅次于晚疫病。目前我国马铃薯栽培品种缺少青枯病抗源,而存在于相关种中的优良抗性资源由于有性杂交不亲和又难以利用。因此,本研究旨在利用对称及非对称融合技术,将抗青枯病茄子优良抗性基因染色体片段整合到马铃薯栽培种中,创制抗青枯病马铃薯新资源。同时期望利用非对称融合技术转移马铃薯染色体片段到茄子中,建立马铃薯染色体片段导入系,为马铃薯遗传基础研究搭建新平台。取得的主要研究结果如下：
1.亲本原生质体培养体系建立研究在对亲本倍性确认的基础上,采用本实验室建立的马铃薯原生质体培养方法,选用7个马铃薯基因型进行原生质体培养研究。结果表明,不同基因型间培养反应差异较大。前期液体浅层培养反应较好的只有3个基因型,包括8#、AC142、AC239.后续培养中,分化培养只有AC142能再生植株。此外,马铃薯二倍体野生种S.chacoence(C9701)虽然前期培养生长缓慢,但在继续培养中可以分化植株。
茄子E508来源于抗青枯病基因型“本地红茄"的种子,组织培养下无性繁殖用于原生质体培养体系建立。使用不同的预处理方法,培养基和激素组合,研究显示,培养4周的试管苗,采用CM培养液黑暗预处理24h,适宜于茄子原生质体分离；CM.-I为适于茄子原生质体前期培养的培养基；后续培养的适宜激素组合为1.0mg,L NAA+0.1mg/L2,4.D+0.25mg/L ZT+0.25mg/LBA。
2.马铃薯-茄子对称融合及其体细胞杂种鉴定研究以二倍体马铃薯AC142和茄子E508为融合亲本,进行原生质体对称融合,共获得了n7个再生植株。使用15对马铃薯SSR标记引物和6对茄子SSR标记引物,鉴定确认了34个株系为体细胞杂种。流式细胞仪分析了17个体细胞杂种的倍性水平,结果显示,17个被测杂种株系中,3个系峰值与四倍体马铃薯种相似；8个系含有两个二倍体马铃薯基因组和1个二倍体茄子基因组(六倍体)；另有6个非整倍体。对一个与马铃薯四倍体峰值相似的系PE29-1和一个按峰值推测含有四倍体马铃薯基因组和二倍体茄子基因组的杂种PE3-4,进行染色体计数,结果证明PE3-4染色体数为72,PE29-1染色体数为48,与流式细胞仪测定结果相符。
3.对称融合体细胞杂种染色体组成分别选取7个体细胞杂种(4个六倍体,1个四倍体和2个非整倍体),采用基因组原位杂交(GISH)分析了其染色体组成。结果显示,4个六倍体杂种均含有48条马铃薯染色体和24条茄子染色体。非整倍体的体细胞杂种PE60-10含54条马铃薯染色体,16条茄子染色体和7条重排染色体。对标记检测为杂种、倍性分析为四倍体的PE29-1进行GISH分析,结果未检测到茄子的杂交信号。上述结果表明,在马铃薯-茄子的融合中,即使是对称融合,也很难获得具有二倍体马铃薯和二倍体茄子染色体的杂种,二倍体马铃薯在体细胞杂种中更容易加倍为为四倍体,这种加倍是两个马铃薯细胞和一个茄子细胞融合产生、还是在培养过程中马铃薯染色体的加倍目前还不清楚。在非整倍体杂种中更容易发生染色体重排。
4.对称杂种的胞质组成研究采用在双亲间具有多态性的线粒体引物P4(pumD)分析了34个体细胞杂种的线粒体组成。结果显示,除了PE29-4和PE57-4两个株系只含有马铃薯亲本带以外,其它32个杂种株系线粒体均为双亲带型,且在所有含有双亲带型的杂种均出现了新带型,表明双亲线粒体基因组在融合后进行了整合。选用两个亲本间具有多态性的叶绿体引物NTCP9和NTCP12,对体细胞杂种叶绿体基因组组成进行了分析。结果显示34个体细胞杂种叶绿体扩增带型均与马铃薯亲本一致。上述结果说明,在体细胞杂种中,线粒体更容易发生重组,而叶绿体则只能保留一个亲本的基因组,且可能偏向远缘融合中提供核背景较多的亲本一方。
5.对称杂种的青枯病抗性评价本研究对11个生根正常的体细胞杂种初步进行了青枯病抗性评价。使用青枯菌1号生理小种对杂种株系试管茵进行伤根接种,9个株系表现为抗青枯病,其中PE4-1的抗性显著高于抗病茄子亲本,其余8个系与之相当。选择试管苗接种后呈现抗性且钵栽生长正常的3个株系进行室外接种,3个株系抗性水平与茄子亲本相当,表明马铃薯-茄子原生质体融合成功地将茄子的青枯病抗性转移到马铃薯中。
6.马铃薯-茄子非对称融合及其融合子鉴定研究采用紫外线照射原生质体破坏供体亲本的染色体结构,以利于融合中的染色体片段插入。
以茄子E508为供体、马铃薯四倍体栽培种8拌为受体的非对称融合中,共获得73个再生株系。使用流式细胞仪对47个株系进行倍性分析,结果表明,36个株系为四倍体,6个为混倍体,2个为非整倍体,1个为八倍体,还有2个株系在测定过程分别出现主峰数量减少和峰值改变的现象,说明在继代过程中植株的染色体可能发生丢失。对16个非对称融合再生株系形态特征进行了观察评价,它们均能结薯,但在薯形、叶片形态、长势等性状上具有不同程度的变异。这些变异是否因为茄子的染色体片段插入而引起,还有待进一步研究。
对马铃薯栽培种DH讷16为供体、茄子E508为受体的非对称融合中,共获得335个愈伤组织,到论文撰写时获得12个再生植株。由于大部分再生株系生根困难,生长缓慢,本研究只能对再生较早、能够生根的3个株系进行了鉴定分析。选用STI024、STI046和STM10883对在亲本间具有有多态性的引物进行分析,3个再生株系均含有马铃薯和茄子特异带,表明它们为体细胞杂种。胞质分析表明,杂种株系的线粒体为双亲融合型,叶绿体为偏马铃薯亲本类型。
Somatic hybridization can not only get rid of sexual incompatibility, but also promote transfer of desire traits intra-/inter-species or inter-genus by fusion simultaneously the nuclear and cytoplasmic genes. This strategy has been considered irreplaceable by conventional crossing in enhancement of plant germplasms to enrich the genetic resources of modern cultivars. Bacterial wilt caused by Ralstonia solanacereaum is an important disease of Solanaceae plants, its impacts on potato is just after late blight. It is lack of resistance resources in solanum tuberosum, and difficulties exist in efficient use of wild species-derived resistance due to cross incompatibility. Therefore, for improving potato resistance, present research aimed to integrate eggplant chromosome fragment carrying bacterial wilt resistance genes into potato by symmetric and asymmetric protoplast fusion. Meanwhile, the eggplant introgression lines by incorporating potato chromosome fragments could be obtained through asymmetric fusion strategy to establish a novel platform for potato genetics. Main results gained are as bellow.
1. Establishment of protoplast culture system for fusion parents. Based on ploidy confirmation of potatoes, with previously developed method, seven potato genotypes were chosen for the protoplast culture. The results showed that response to the culture conditions was genotype-dependent indicated by difference in callus formation during the shallow-liquid culture phase at the beginning of protoplast culture. Three genotypes,8#, AC142and AC239, performed better than others. Only AC142regenerated plants in the following culture procedure. In addition, C9701, a succession of S. chacoense, could form plants although it grew slowly at early stage of the culture.
The eggplant parent E508with bacterial wilt resistance was derived from seed of a cultivar "BendiHongqie". The in vitro grown plantlets were used for the protoplast culture. After test of the method for priming and combination of media and hormones, the protocol was optimized that a24h dark priming in CM medium of the leaves taken from 4week-old plants was available for protoplast isolation, CM-I medium was desire for early stage of the protoplast culture, and the combination of1.0mg/1NAA+0.1mg/12,4-D+0.25mg/l ZT+0.25mg/1BA was favorite for late stage culture.
2. Fusion and somatic hybrids identification of potato-eggplant. The symmetric protoplast fusion was conducted between a diploid potato AC142and eggplant E508. Thirty-four of117regenerated plants were identified somatic hybrids with15potato specific SSR markers and6eggplant SSR specific markers. Seventeen hybrids first obtained were subjected to ploidy test by flow cytometer. The results, showed that, by aligning to the absorbent peaks of the parents,3hybrids were similar to potato tetraploid,8(hexaploid) were combination of2diploid potato and1eggplant genomes, and the rest6denoted aneuploid. The chromosome counting of hybrid PE29-1(showed peak value of potato tetraploid) and PE3-4(showed peak value of tetraploid potato+diploid eggplant) conformed the flow cytometer results that PE29-1has48chromosomes while PE3-4has72.
3. Chromosome composition of symmetric hybrids. The genome in situ hybridization (GISH) was applied to7somatic hybrids (4hexaploids,1tetraploid,2aneuploid) to elucidate their chromosome composition. The results demonstrated that the hexaploids consisted of48potato chromosomes and24eggplant chromosomes, while the aneupolid PE60-10contained54potato chromosomes,16eggplant chromosomes and7rearranged chromosomes. However, the tetraploid hybrid PE29-1identified by SSR markers failed to observe the signal of eggplant chromosomes by GISH. The above findings indicated rare tetraploids derived from diploid potato and diploid eggplant even for symmetric fusion. The diploid potato is likely to be doubled in the process of protoplast fusion, for example in hexaploid hybrids, but it remains unknown whether this doubling comes from a fusion of two diploid potato cells and a diploid eggplant cell, or the diploid potato cell is doubled during the process of protoplast culture. It seems that the chromosome rearrangement is in preference to occur in aneuploids.
4. Cytoplasm composition of symmetric hybrids. The polymorphic mitochondria primer P4(pumD) was employed to dissect the mitochondrion components of34somatic hybrid. Except for PE29-4and PE57-4that contained only potato bands, all hybrids showed integration of the bands from two fusion parents. Furthermore, there were novel bands detected, indicating recombination of parental mitochondria genomes after fusion. Two polymorphic chloroplast markers, NTCP9and NTCP12, were used for chloroplast genome analysis of the hybrids. Interestingly, all34hybrids showed only potato chloroplast. Present results suggested that, in somatic hybrids obtained, mitochondria are often to recombine, whereas the chloroplast of one parent is more capable to retain than the other, this may occur preferentially to the parent contributing dominant DNA dosage in the hybrids.
5. Bacterial wilt resistance of symmetric hybrids. The disease assessment was carried out with11somatic hybrids rooted normally. The plantlets grown in vitro were inoculated with cell suspension of R. solanacearum race1. There9hybrids scored resistance, of which PE4-1showed a higher resistance level than the resistant eggplant parent and other8hybrids had no difference compared to the resistant control. Three hybrids which could grow normally in pots and showed resistance in vitro were tested for resistance to bacterial wilt. Three inoculated somatic hybrids showed high resistance similar to the eggplant fusion parent. These results demonstrate that the protoplast fusion is a feasible approach to successfully transfer bacterial wilt resistance from eggplant to potato.
6. Asymmetric fusion of potato+eggplant and identification of somatic hybrids. Eggplant E508was exposed to UV light treatment to breakdown the chromosomes as the donor to fuse with potato protoplast of8#as recipient. Forty-seven of73regenerated plants were assayed with the flow cytometer and the results showed36tetraploids similar to potato parent8#,6mixoploids,2aneuploids and1octaploid, as well as2with varied peak values that may be resulted from chromosome elimination during the subcultures.
The variations in plant morphology of16regenerated plants were observed in tuber shape, leaf shape and growth vigour. However, all the16regenerated plants can produce tubers normally. It worth further clarifying if the morphology change comes from the insertion of eggplant chromosome fragments.
In the fusion with eggplant E508as recipient and UV-treated dihaploid potato Ne16as doner,12plants were generated from335calli. Since most of them were difficult in rooting and slow in growth, only3rooted plants were taken for further analysis. Amplification with3polymorphic markers, STI024, STI046and STM1088, confirmed the rooted plants were somatic hybrids. Furthermore, the cytoplasmic analysis revealed that these hybrids exhibited an integrated mitochondrion pattern of the parents and a potato chloroplast pattern.
1.亲本原生质体培养体系建立研究在对亲本倍性确认的基础上,采用本实验室建立的马铃薯原生质体培养方法,选用7个马铃薯基因型进行原生质体培养研究。结果表明,不同基因型间培养反应差异较大。前期液体浅层培养反应较好的只有3个基因型,包括8#、AC142、AC239.后续培养中,分化培养只有AC142能再生植株。此外,马铃薯二倍体野生种S.chacoence(C9701)虽然前期培养生长缓慢,但在继续培养中可以分化植株。
茄子E508来源于抗青枯病基因型“本地红茄"的种子,组织培养下无性繁殖用于原生质体培养体系建立。使用不同的预处理方法,培养基和激素组合,研究显示,培养4周的试管苗,采用CM培养液黑暗预处理24h,适宜于茄子原生质体分离；CM.-I为适于茄子原生质体前期培养的培养基；后续培养的适宜激素组合为1.0mg,L NAA+0.1mg/L2,4.D+0.25mg/L ZT+0.25mg/LBA。
2.马铃薯-茄子对称融合及其体细胞杂种鉴定研究以二倍体马铃薯AC142和茄子E508为融合亲本,进行原生质体对称融合,共获得了n7个再生植株。使用15对马铃薯SSR标记引物和6对茄子SSR标记引物,鉴定确认了34个株系为体细胞杂种。流式细胞仪分析了17个体细胞杂种的倍性水平,结果显示,17个被测杂种株系中,3个系峰值与四倍体马铃薯种相似；8个系含有两个二倍体马铃薯基因组和1个二倍体茄子基因组(六倍体)；另有6个非整倍体。对一个与马铃薯四倍体峰值相似的系PE29-1和一个按峰值推测含有四倍体马铃薯基因组和二倍体茄子基因组的杂种PE3-4,进行染色体计数,结果证明PE3-4染色体数为72,PE29-1染色体数为48,与流式细胞仪测定结果相符。
3.对称融合体细胞杂种染色体组成分别选取7个体细胞杂种(4个六倍体,1个四倍体和2个非整倍体),采用基因组原位杂交(GISH)分析了其染色体组成。结果显示,4个六倍体杂种均含有48条马铃薯染色体和24条茄子染色体。非整倍体的体细胞杂种PE60-10含54条马铃薯染色体,16条茄子染色体和7条重排染色体。对标记检测为杂种、倍性分析为四倍体的PE29-1进行GISH分析,结果未检测到茄子的杂交信号。上述结果表明,在马铃薯-茄子的融合中,即使是对称融合,也很难获得具有二倍体马铃薯和二倍体茄子染色体的杂种,二倍体马铃薯在体细胞杂种中更容易加倍为为四倍体,这种加倍是两个马铃薯细胞和一个茄子细胞融合产生、还是在培养过程中马铃薯染色体的加倍目前还不清楚。在非整倍体杂种中更容易发生染色体重排。
4.对称杂种的胞质组成研究采用在双亲间具有多态性的线粒体引物P4(pumD)分析了34个体细胞杂种的线粒体组成。结果显示,除了PE29-4和PE57-4两个株系只含有马铃薯亲本带以外,其它32个杂种株系线粒体均为双亲带型,且在所有含有双亲带型的杂种均出现了新带型,表明双亲线粒体基因组在融合后进行了整合。选用两个亲本间具有多态性的叶绿体引物NTCP9和NTCP12,对体细胞杂种叶绿体基因组组成进行了分析。结果显示34个体细胞杂种叶绿体扩增带型均与马铃薯亲本一致。上述结果说明,在体细胞杂种中,线粒体更容易发生重组,而叶绿体则只能保留一个亲本的基因组,且可能偏向远缘融合中提供核背景较多的亲本一方。
5.对称杂种的青枯病抗性评价本研究对11个生根正常的体细胞杂种初步进行了青枯病抗性评价。使用青枯菌1号生理小种对杂种株系试管茵进行伤根接种,9个株系表现为抗青枯病,其中PE4-1的抗性显著高于抗病茄子亲本,其余8个系与之相当。选择试管苗接种后呈现抗性且钵栽生长正常的3个株系进行室外接种,3个株系抗性水平与茄子亲本相当,表明马铃薯-茄子原生质体融合成功地将茄子的青枯病抗性转移到马铃薯中。
6.马铃薯-茄子非对称融合及其融合子鉴定研究采用紫外线照射原生质体破坏供体亲本的染色体结构,以利于融合中的染色体片段插入。
以茄子E508为供体、马铃薯四倍体栽培种8拌为受体的非对称融合中,共获得73个再生株系。使用流式细胞仪对47个株系进行倍性分析,结果表明,36个株系为四倍体,6个为混倍体,2个为非整倍体,1个为八倍体,还有2个株系在测定过程分别出现主峰数量减少和峰值改变的现象,说明在继代过程中植株的染色体可能发生丢失。对16个非对称融合再生株系形态特征进行了观察评价,它们均能结薯,但在薯形、叶片形态、长势等性状上具有不同程度的变异。这些变异是否因为茄子的染色体片段插入而引起,还有待进一步研究。
对马铃薯栽培种DH讷16为供体、茄子E508为受体的非对称融合中,共获得335个愈伤组织,到论文撰写时获得12个再生植株。由于大部分再生株系生根困难,生长缓慢,本研究只能对再生较早、能够生根的3个株系进行了鉴定分析。选用STI024、STI046和STM10883对在亲本间具有有多态性的引物进行分析,3个再生株系均含有马铃薯和茄子特异带,表明它们为体细胞杂种。胞质分析表明,杂种株系的线粒体为双亲融合型,叶绿体为偏马铃薯亲本类型。
Somatic hybridization can not only get rid of sexual incompatibility, but also promote transfer of desire traits intra-/inter-species or inter-genus by fusion simultaneously the nuclear and cytoplasmic genes. This strategy has been considered irreplaceable by conventional crossing in enhancement of plant germplasms to enrich the genetic resources of modern cultivars. Bacterial wilt caused by Ralstonia solanacereaum is an important disease of Solanaceae plants, its impacts on potato is just after late blight. It is lack of resistance resources in solanum tuberosum, and difficulties exist in efficient use of wild species-derived resistance due to cross incompatibility. Therefore, for improving potato resistance, present research aimed to integrate eggplant chromosome fragment carrying bacterial wilt resistance genes into potato by symmetric and asymmetric protoplast fusion. Meanwhile, the eggplant introgression lines by incorporating potato chromosome fragments could be obtained through asymmetric fusion strategy to establish a novel platform for potato genetics. Main results gained are as bellow.
1. Establishment of protoplast culture system for fusion parents. Based on ploidy confirmation of potatoes, with previously developed method, seven potato genotypes were chosen for the protoplast culture. The results showed that response to the culture conditions was genotype-dependent indicated by difference in callus formation during the shallow-liquid culture phase at the beginning of protoplast culture. Three genotypes,8#, AC142and AC239, performed better than others. Only AC142regenerated plants in the following culture procedure. In addition, C9701, a succession of S. chacoense, could form plants although it grew slowly at early stage of the culture.
The eggplant parent E508with bacterial wilt resistance was derived from seed of a cultivar "BendiHongqie". The in vitro grown plantlets were used for the protoplast culture. After test of the method for priming and combination of media and hormones, the protocol was optimized that a24h dark priming in CM medium of the leaves taken from 4week-old plants was available for protoplast isolation, CM-I medium was desire for early stage of the protoplast culture, and the combination of1.0mg/1NAA+0.1mg/12,4-D+0.25mg/l ZT+0.25mg/1BA was favorite for late stage culture.
2. Fusion and somatic hybrids identification of potato-eggplant. The symmetric protoplast fusion was conducted between a diploid potato AC142and eggplant E508. Thirty-four of117regenerated plants were identified somatic hybrids with15potato specific SSR markers and6eggplant SSR specific markers. Seventeen hybrids first obtained were subjected to ploidy test by flow cytometer. The results, showed that, by aligning to the absorbent peaks of the parents,3hybrids were similar to potato tetraploid,8(hexaploid) were combination of2diploid potato and1eggplant genomes, and the rest6denoted aneuploid. The chromosome counting of hybrid PE29-1(showed peak value of potato tetraploid) and PE3-4(showed peak value of tetraploid potato+diploid eggplant) conformed the flow cytometer results that PE29-1has48chromosomes while PE3-4has72.
3. Chromosome composition of symmetric hybrids. The genome in situ hybridization (GISH) was applied to7somatic hybrids (4hexaploids,1tetraploid,2aneuploid) to elucidate their chromosome composition. The results demonstrated that the hexaploids consisted of48potato chromosomes and24eggplant chromosomes, while the aneupolid PE60-10contained54potato chromosomes,16eggplant chromosomes and7rearranged chromosomes. However, the tetraploid hybrid PE29-1identified by SSR markers failed to observe the signal of eggplant chromosomes by GISH. The above findings indicated rare tetraploids derived from diploid potato and diploid eggplant even for symmetric fusion. The diploid potato is likely to be doubled in the process of protoplast fusion, for example in hexaploid hybrids, but it remains unknown whether this doubling comes from a fusion of two diploid potato cells and a diploid eggplant cell, or the diploid potato cell is doubled during the process of protoplast culture. It seems that the chromosome rearrangement is in preference to occur in aneuploids.
4. Cytoplasm composition of symmetric hybrids. The polymorphic mitochondria primer P4(pumD) was employed to dissect the mitochondrion components of34somatic hybrid. Except for PE29-4and PE57-4that contained only potato bands, all hybrids showed integration of the bands from two fusion parents. Furthermore, there were novel bands detected, indicating recombination of parental mitochondria genomes after fusion. Two polymorphic chloroplast markers, NTCP9and NTCP12, were used for chloroplast genome analysis of the hybrids. Interestingly, all34hybrids showed only potato chloroplast. Present results suggested that, in somatic hybrids obtained, mitochondria are often to recombine, whereas the chloroplast of one parent is more capable to retain than the other, this may occur preferentially to the parent contributing dominant DNA dosage in the hybrids.
5. Bacterial wilt resistance of symmetric hybrids. The disease assessment was carried out with11somatic hybrids rooted normally. The plantlets grown in vitro were inoculated with cell suspension of R. solanacearum race1. There9hybrids scored resistance, of which PE4-1showed a higher resistance level than the resistant eggplant parent and other8hybrids had no difference compared to the resistant control. Three hybrids which could grow normally in pots and showed resistance in vitro were tested for resistance to bacterial wilt. Three inoculated somatic hybrids showed high resistance similar to the eggplant fusion parent. These results demonstrate that the protoplast fusion is a feasible approach to successfully transfer bacterial wilt resistance from eggplant to potato.
6. Asymmetric fusion of potato+eggplant and identification of somatic hybrids. Eggplant E508was exposed to UV light treatment to breakdown the chromosomes as the donor to fuse with potato protoplast of8#as recipient. Forty-seven of73regenerated plants were assayed with the flow cytometer and the results showed36tetraploids similar to potato parent8#,6mixoploids,2aneuploids and1octaploid, as well as2with varied peak values that may be resulted from chromosome elimination during the subcultures.
The variations in plant morphology of16regenerated plants were observed in tuber shape, leaf shape and growth vigour. However, all the16regenerated plants can produce tubers normally. It worth further clarifying if the morphology change comes from the insertion of eggplant chromosome fragments.
In the fusion with eggplant E508as recipient and UV-treated dihaploid potato Ne16as doner,12plants were generated from335calli. Since most of them were difficult in rooting and slow in growth, only3rooted plants were taken for further analysis. Amplification with3polymorphic markers, STI024, STI046and STM1088, confirmed the rooted plants were somatic hybrids. Furthermore, the cytoplasmic analysis revealed that these hybrids exhibited an integrated mitochondrion pattern of the parents and a potato chloroplast pattern.
引文
1.蔡兴奎,柳俊,谢从华.马铃薯栽培种与野生种叶肉细胞融合及体细胞杂种鉴定.园艺学报,2004,31：623-626
2.蔡兴奎：原生质体融合创造抗青枯病的马铃薯新种质及其遗传分析.[博士学位论文].武汉：华中农业大学图书馆,2003
3.陈文品,吴琴生,刘大钧.小麦与多年生黑麦草原生质体的电融合及杂种愈伤组织的形成.植物学报,1992,34：284-290
4.郭文武,周长河,伊华林,邓秀新.柑橘与枳属间体细胞杂种再生及其对脚腐病抗性的评价.植物学报,2000,42：668-672
5.刘喜才,张丽娟：马铃薯种质资源描述规范和数据标准.中国农业出版社,2006
6.宋锡全,夏光敏,周爱芬,鲍雪珍,陈惠民.葡萄与柴胡科间体细胞杂交再生杂种植株.科学通报,1999,44：1832-1835
7.孙慧生.马铃薯育种学.中国农业出版社,2003
8.王槐,陈正华,夏光敏,陈惠民.植物体细胞杂交的进展.生命科学,1999,11：100-103
9.吴田.马铃薯晚疫病水平抗性相关基因STPKl, STLRPKl, Star的克隆和功能分析.[博士学位论文].武汉：华中农业大学图书馆,2009.
10.夏光敏,陈惠民.小麦与高冰草原生质体融合及再生能力的恢复.山东大学学报,自然科学版,1995,30：325-330
11.夏光敏,陈穗云,向凤宁,权太勇,陈惠民.小麦与高冰草体细胞杂种F-3-F-5代的耐盐性研究.山东农业科学,2001：12-14
12.夏光敏,王槐,陈惠民.小麦与新麦草及高冰草属间不对称体细胞杂交的植株再生.科学通报,1996,41：1423-1426
13.夏光敏,向凤宁,周爱芬,王槐,何世贤,陈惠民.小麦与高冰草属间体细胞杂交获可育杂种植株.植物学报,1999,41：349-352
14.周爱芬,夏光敏,陈惠民.普通小麦与簇毛麦不对称体细胞杂交的研究.巾国科学C辑：生命科学,1996,26：31-37
15.周传恩.利用不对称体细胞杂交技术构建普通小麦辐射杂种嵌板及其图谱的研究.[博士学位论文].济南：山东大学图书馆,2007
16.周宇波.马铃薯原生质体培养及其再生植株的遗传变异研究.[硕士学位论文].武汉：华中农业大学图书馆,2000
17. Abdullah R, Thompson J, Khush G, Kaushik R, Cocking E. Protoclonal variation in the seed progeny of plants regenerated from rice protoplasts. Plant Sci,1989,65: 97-101
18. Austin S, Baer MA, Helgeson JP. Transfer of resistance to potato leaf roll virus from Solanum brevidens into Solanum tuberosum by somatic fusion. Plant Sci,1985,39: 75-81
19. Baldev A, Gaikwad K, Kirti P, Mohapatra T, Prakash S, Chopra V. Recombination between chloroplast genomes of Trachystoma ballii and Brassica juncea following protoplast fusion. Mol Gen Genet,1998,260:357-361
20. Bamberg J, Hanneman Jr R, Palta J, Harbage J. Using disomic 4x(2EBN) potato species'germplasm via bridge species Solanum commersonii. Genome,1994,37: 866-870
21. Bastia T, Carotenuto N, Basile B, Zoina A, Cardi T. Induction of novel organelle DNA variation and transfer of resistance to frost and Verticillium wilt in Solanum tuberosum through somatic hybridization with 1EBN 51 commersonii. Euphytica, 2000,116:1-10
22. Bates G, Hasenkampf C, Contolini C, Piastuch W. Asymmetric hybridization in Nicotiana by fusion of irradiated protoplasts. Theor Appl Genet,1987,74:718-726
23. Bauer-Weston B, Keller W, Webb J, Gleddie S. Production and characterization of asymmetric somatic hybrids between Arabidopsis thaliana and Brassica napus. Theor Appl Genet,1993,86:150-158
24. Bidani A, Nouri-Ellouz O, Lakhoua L, Sihachakr D, Cheniclet C, Mahjoub A, Drira N, Gargouri-Bouzid R. Interspecific potato somatic hybrids between Solanum berthaultii and Solanum tuberosum L. showed recombinant plastome and improved tolerance to salinity. Plant Cell Tiss Org,2007,91:179-189
25. Bohman S, Wang M, Dixelius C. Arabidopsis thaliana-derived resistance against Leptosphaeria maculans in a Brassica napus genomic background. Theor Appl Genet,2002,105:498-504
26. Borgato L, Conicella C, Pisani F, Furini A. Production and characterization of arboreous and fertile Solanum melongena+ Solamum marginatum somatic hybrid plants. Planta,2007,226:961-969
27. Brewer E, Saunders J, Angle JS, Chaney R, McIntosh M. Somatic hybridization between the zinc accumulator Thlaspi caerulescens and Brassica napus. Theor Appl Genet,1999,99:761-771
28. Bryan G, McNicoll J, Ramsay G, Meyer R, De Jong W. Polymorphic simple sequence repeat markers in chloroplast genomes of Solanaceous plants. Theor Appl Genet,1999,99:859-867
29. Cardi T, D'Ambrosio E, Consoli D, Puite K, Ramulu KS. Production of somatic hybrids between frost-tolerant Solanum commersonii and S.tuberosum: characterization of hybrid plants. Theor Appl Genet,1993,87:193-200
30. Carlson PS, Smith HH, Dealing RD. Parasexual interspecific plant hybridization. Proc Natl Acad Sci,1972,69:2292-2294
31. Carputo D, Barone A, Cardi T, Sebastiano A, Frusciante L, Peloquin SJ. Endosperm balance number manipulation for direct in vivo germplasm introgression to potato from a sexually isolated relative(Solanum commersonii Dun.). Proc Natl Acad Sci, 1997,94:12013-12017
32. Cheng A, Xia G. Somatic hybridisation between common wheat and Italian ryegrass. Plant Sci,2004b,166:1219-1226
33. Cheng AX, Xia GM, Zhi DY, CHEN HM. Intermediate fertile Triticum aestivum (+) Agropyron elongatum somatic hybrids are generated by low doses of UV irradiation. Cell Res,2004a,14:86-91
34. Collonnier C, Fock I, Mariska I, Servaes A, Vedel F, Siljak-Yakovlev S, Souvannavong V, Sihachakr D. GISH confirmation of somatic hybrids between Solanum melongena and S. torvum:assessment of resistance to both fungal and bacterial wilts. Plant Physiol Biochem,2003,41:459-470
35. Collonnier C, Mulya K, Fock I, Mariska I, Servaes A, Vedel F, Siljak-Yakovlev S, Souvannavong V, Ducreux G, Sihachakr D. Source of resistance against Ralstonia solanacearum in fertile somatic hybrids of eggplant Solanum melongena with Solanum aethiopicum L. Plant Sci,2001,160:301-313
36. Cui H, Yu Z, Deng J, Gao X, Sun Y, Xia G. Introgression of bread wheat chromatin into tall wheatgrass via somatic hybridization. Planta,2009,229:323-330
37. Daunay M, Chaput M, Sihachakr D, Allot M, Vedel F, Ducreux G. Production and characterization of fertile somatic hybrids of eggplant(Solanum melongena L.) with Solanum aethiopicum L. Theor Appl Genet,1993,85:841-850
38. Demesure B, Sodzi N, Petit R. A set of universal primers for amplification of polymorphic non-coding regions of mitochondrial and chloroplast DNA in plants. Mol Ecol,1995,4:129-131
39. Deng JY, Cui HF, Zhi DY, Zhou CN, Xia GM. Analysis of remote asymmetric somatic hybrids between common wheat and Arabidopsis thaliana. Plant Cell Rep, 2007,26:1233-1241
40. Dudits D, Fejer O, Hadlaczky G, Koncz C, Lazar GB, Horvath G. Intergeneric gene transfer mediated by plant protoplast fusion. Mol Gen Genet,1980,179:283-288
41. Dumolin-Lapegue S, Pemonge M, Petit R. An enlarged set of consensus primers for the study of organelle DNA in plants. Mol Ecol,1997,6:393-397
42. Ehlenfeldt M, Helgeson J. Fertility of somatic hybrids from protoplast fusions of Solanum brevidens and & tuberosum. Theor Appl Genet,1987,73:395-402
43. Elphinstone, JG Inheritance of resistance to bacterial diseases. In:Bradshaw JE, Mackay GR eds., Potato genetics. CAB International, Wallingford,1994.429-446
44. Fahleson J, Eriksson I, Landgren M, Stymne S, Glimelius K. Intertribal somatic hybrids between Brassica napus and Thlaspi perfoliatum with high content of the T. perfoliatum-specific nervonic acid. TheorAppl Genet,1994,87:795-804
45. Fan LM, Wang YF, Wu WH. Outward K+ channels in Brassica chinensis pollen protoplasts are regulated by external and internal pH. Protoplasma,2003,220: 143-152
46. Feingold S, Lloyd J, Norero N, Bonierbale M, Lorenzen J. Mapping and characterization of new EST-derived microsatellites for potato(Solanum tuberosum L.). Theor Appl Genet,2005,111:456-466
47. Fock I, Collonnier C, Luisetti J, Purwito A, Souvannavong V, Vedel F, Servaes A, Ambroise A, Kodja H, Ducreux G, Sihachakr D. Use of Solanum stenotomum for introduction of resistance to bacterial wilt in somatic hybrids of potato. Plant Physiol Biochem,2001,39:899-908
48. Fock I, Collonnier C, Purwito A, Luisetti J, Souvannavong V, Vedel F, Servaes A, Ambroise A, Kodja H, Ducreux G, Sihachakr D. Resistance to bacterial wilt in somatic hybrids between Solanum tuberosum and Solanum phureja. Plant Sci,2000, 160:165-176
49. Forsberg J, Lagercrantz U, Glimelius K. Comparison of UV light, X-ray and restriction enzyme treatment as tools in production of asymmetric somatic hybrids between Brassica napus and Arabidopsis thaliana. Theor Appl Genet,1998,96: 1178-1185
50. Gaikwad K, Kirti P, Sharma A, Prakash S, Chopra V. Cytogenetical and molecular investigations on somatic hybrids of Sinapis alba and Brassica juncea and their backcross progeny. Plant Breeding,1996,115:480-483
51. Gavrilenko T, Thieme R, Rokka V-M. Cytogenetic analysis of Lycopersicon esculentum (+) Solanum etuberosum somatic hybrids and their androgenetic regenerants. Theor Appl Genet,2001,103:231-239
52. Gavrilenko T, Thieme R, Tiemann H. Assessment of genetic and phenotypic variation among intraspecific somatic hybrids of potato, Solanum tuberosum L. Plant Breeding,1999,118:205-213
53. Gibson R, Jones M, Fish N. Resistance to potato leaf roll virus and potato virus Y in somatic hybrids between dihaploid Solanum tuberosum and S. brevidens. Theor Appl Genet,1988,76:113-117
54. Gilissen L, Staveren Mv, Verhoeven H, Sree Ramulu K. Somatic hybridization between potato and Nicotiana plumbaginifolia. Theor Appl Genet,1992,84:73-80
55. Gleddie S, Fassuliotis G, Keller W, Setterfield G. Somatic hybridization as a potential method of transferring nematode and mite resistance into eggplant. Z Pflanzenzuchtung,1985,94:352-355
56. Gleddie S, Keller W, Setterfield G.Production and characterization of somatic hybrids between Solanum melongena L. and S. sisymbriifolium Lam. Theor Appl Genet,1986,71:613-621
57. Greplova M, Polzerova H, Vlastnikova H. Electrofusion of protoplasts from Solamum tuberosum, S. bulbocastanum and S. pinnatisectum. Acta Physiol Plant,2008,30: 787-796
58. Guo W, Deng X. Somatic hybrid plantlets regeneration between Citrus and its wild relative, Murraya paniculata via protoplast electrofusion. Plant Cell Rep,1998,18: 297-300
59. Guo XP, Xie CH, Cai XK, Song BT, He L, Liu J. Meiotic behavior of pollen mother cells in relation to ploidy level of somatic hybrids between Solanum tuberosum and S. chacoense. Plant Cell Rep,2010,29:1277-1285
60. Guri A, Sink K. Interspecific somatic hybrid plants between eggplant (Solanum melongena) and Solanum torvum. Theor Appl Genet,1988a,76:490-496
61. Guri A, Sink K. Organelle composition in somatic hybrids between an atrazine resistant biotype of Solanum nigrum and Solanum melongena. Plant Sci,1988b,58: 51-58
62. Hansen L, Earle E. Somatic hybrids between Brassica oleracea L. and Sinapis alba L. with resistance to Alternaria brassicae (Berk.) Sacc. Theor Appl Genet,1997,94: 1078-1085
63. Hayward AC. Biology and epidemiology of bacterial wilt caused by Pseudomonas solanacearum. Annu Rev Phytopathol,1991,29:65-87
64. Helgeson J, Pohlman J, Austin S, Haberlach G, Wielgus S, Ronis D, Zambolim L, Tooley P, McGrath J, James R. Somatic hybrids between Solanum bulbocastanum and potato:a new source of resistance to late blight. Theor Appl Genet,1998,96: 738-742
65. Helgeson JP, Haberlach GT:Somatic hybrids of Solanum tuberosum and related species. In Plant Biotechnology and In Vitro Biology in the 21st Century. Springer; 1999:151-154:
66. Hoffmann F, Adachi T. "Arabidobrassica":Chromosomal recombination and morphogenesis in asymmetric intergeneric hybrid cells. Planta,1981,153:586-593
67. Horsman K, Gavrilenko T, Bergervoet M, Huigen DJ, Joe A, Jacobsen E. Alteration of the genomic composition of Solanum nigrum (+) potato backcross derivatives by somatic hybridization:selection of fusion hybrids by DNA measurements and GISH. Plant Breeding,2001,120:201-207
68. Hu Q, Hansen L, Laursen J, Dixelius C, Andersen S. Intergeneric hybrids between Brassica napus and Orychophragmus violaceus containing traits of agronomic importance for oilseed rape breeding. Theor Appl Genet,2002,105:834-840
69. Ishikawa S, Bang S, Kaneko Y, Matsuzawa Y, Robbelen G. Production and characterization of intergeneric somatic hybrids between Moricandia arvensis and Brassica oleracea. Plant Breeding,2003,122:233-238
70. Jarl C, Rietveld E, De Haas J. Transfer of fungal tolerance through interspecific somatic hybridisation between Solanum melongena and S. torvum. Plant Cell Rep, 1999,18:791-796
71. Jelenic S, Berljak J, Papes D, Jelaska S. Mixoploidy and chimeric structures in somaclones of potato (Solanum tuberosum L.) cv. Bintje. Food Technol Biotech, 2001,39:13-18
72. Johnston S, Hanneman R. Manipulations of endosperm balance number overcome crossing barriers between diploid Solanum species. Science,1982,217:446-448
73. Kanno A, Kanzaki H, Kameya T. Detailed analyses of chloroplast and mitochondrial DNAs from the hybrid plant generated by asymmetric protoplast fusion between radish and cabbage. Plant Cell Rep,1997,16:479-484
74. Kao KN, Michayluk MR. Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta,1975, 126:105-110
75. Kim H, Chae M, Choi S, Wielgus S, Helgeson J. Identification of somatic hybrids produced by protoplast fusion between Solanum commersonii and S. tuberosum haploid. Korean J Plant Tissue Cult,1993,20:337-344
76. Kim YJ, Kim JE, Lee J-H, Lee MH, Jung HW, Bahk YY, Hwang BK, Hwang I, Kim WT. The Vr-PLC3 gene encodes a putative plasma membrane-localized phosphoinositide-specific phospholipase C whose expression is induced by abiotic stress in mung bean (Vigna radiata L.). FEBS letters,2004,556:127-136
77. Kim-Lee H, Moon J, Hong Y, Kim M, Cho H. Bacterial wilt resistance in the progenies of the fusion hybrids between haploid of potato and Solanum commersonii. Am J Potato Res,2005,82:129-137
78. Kisaka H, Kisaka M, Kanno A, Kameya T. Intergeneric somatic hybridization of rice (Oryza sativa L.) and barley (Hordeum vulgare L.) by protoplast fusion. Plant Cell Rep,1998,17:362-367
79. Kisaka H, Kisaka M, Kanno A, Kameya T. Production and analysis of plants that are somatic hybrids of barley (Hordeum vulgare L.) and carrot (Daucus carota L.). Theor Appl Genet,1997,94:221-226
80. Kostenyuk I, Lubaretz O, Borisyuk N, Voronin V, Stockigt J, Gleba Y. Isolation and characterization of intergeneric somatic hybrids in the Apocynaceae family. Theor Appl Genet,1991,82:713-716
81. Kyozuka J, Kaneda T, Shimamoto K. Production of cytoplasmic male sterile rice (Oryza sativa L.) by cell fusion. Nat Biotechnol,1989,7:1171-1174
82. Laferriere L, Helgeson J, Allen C. Fertile Solanum tuberosum+ S. commersonii somatic hybrids as sources of resistance to bacterial wilt caused by Ralstonia solanacearum. Theor Appl Genet,1999,98:1272-1278
83. Larkin PJ, Scowcroft W. Somaclonal variation-a novel source of variability from cell cultures for plant improvement. Theor Appl Genet,1981,60:197-214
84. Lee SH, Shon YQ Kim CY, Chun HJ, Cheong YH, Kim ZH, Choe ZR, Choi YJ, Cho MJ. Variations in the morphology of rice plants regenerated from protoplasts using different culture procedures. Plant Cell Tiss Org,1999,57:179-187
85. Lelivelt C, Krens F. Transfer of resistance to the beet cyst nematode (Heterodera schachtii Schm.) into the Brassica napus L. gene pool through intergeneric somatic hybridization with Raphanus sativus L. Theor Appl Genet,1992,83:887-894
86. Liang X, Ding S, Wong S. Development of a kenaf (Hibiscus cannabinus L.) protoplast system for a replication study of Hibiscus chlorotic ringspot virus. Plant Cell Rep,2002,20:982-986
87. Liu J, Xu X, Deng X. Intergeneric somatic hybridization and its application to crop genetic improvement. Plant Cell Tiss Org,2005,82:19-44
88. Liu JH, Hu CG, Deng XX. Regeneration of diploid intergeneric somatic hybrid plants between Microcitrus and Citrus via electrofusion. Acta Bot Sin,1999,41:1177-1182
89. Liu JH, Pang XM, Cheng YJ, Meng HJ, Deng X.Molecular characterization of the nuclear and cytoplasmic genomes of intergeneric diploid plants from cell fusion between Microcitrus papuana and Rough lemon. Plant Cell Rep,2002,21:327-332
90. Lossl A, Adler N, Horn R, Frei U, Wenzel G. Chondriome-type characterization of potato:mt α, β, γδε and novel plastid-mitochondrial configurations in somatic hybrids. Theor Appl Genet,1999,99:1-10
91. Melzer J, O'Connell M. Effect of radiation dose on the production of and the extent of asymmetry in tomato asymmetric somatic hybrids. Theor Appl Genet,1992,83: 337-344
92. Menke U, Schilde-Rentschler L, Ruoss B, Zanke C, Hemleben V, Ninnemann H. Somatic hybrids between the cultivated potato Solanum tuberosum L. and the 1EBN wild species Solanum pinnatisectum Dun.:morphological and molecular characterization. Theor Appl Genet,1996,92:617-626
93. Mezencev N, Clement G, Guiderdoni E. Variation among progenies of diploid plants regenerated from haploid, microspore-derived cell-suspension protoplasts of rice (Oryza sativa L.). Plant Breeding,1995,114:149-154
94. Milbourne D, Meyer R, Collins A, Ramsay L, Gebhardt C, Waugh R. Isolation, characterisation and mapping of simple sequence repeat loci in potato. Mol Gen Genet,1998,259:233-245
95. Mliki A, Jardak R, Reustle G, Ghorbel A. Isolation and culture of leaf protoplasts from Tunisian grapes. JInt Sci Vigne Vin,2003,37:145-153
96. Mohapatra T, Kirti P, Kumar VD, Prakash S, Chopra V. Random chloroplast segregation and mitochondrial genome recombination in somatic hybrid plants of Diplotaxis catholica+ Brassica juncea. Plant Cell Rep,1998,17:814-818
97. Moreira CD, Chase CD, Gmitter Jr FG, Grosser JW. Inheritance of organelle genomes in citrus somatic cybrids. Mol Breeding,2000,6:401-405
98. Nouri-Ellouz O, Gargouri-Bouzid R, Sihachakr D, Triki MA, Ducreux G, Drira N, Lakhoua L. Production of potato intraspecific somatic hybrids with improved tolerance to PVY and Pythium aphanidermatum. J Plant Physiol,2006,163: 1321-1332
99. Novy R, Helgeson J. Resistance to potato virus Y in somatic hybrids between Solanum etuberosum and S. tuberosum x S. berthaultii hybrid. Theor Appl Genet, 1994a,89:783-786
100. Novy R, Helgeson J. Somatic hybrids between Solanum etuberosum and diploid, tuber bearing Solanum clones. Theor Appl Genet,1994b,89:775-782
101.Nunome T, Negoro S, Kono I, Kanamori H, Miyatake K, Yamaguchi H, Ohyatna A, Fukuoka H. Development of SSR markers derived from SSR-enriched genomic library of eggplant (Solanum melongena L.). Theor Appl Genet,2009,119: 1143-1153
102.Nyman M, Waara S. Characterisation of somatic hybrids between Solanum tuberosum and its frost-tolerant relative Solanum commersonil. Theor Appl Genet, 1997,95:1127-1132
103.Oberwalder B, Schilde-Rentschler L, Ruofβ B, Wittemann S, Ninnemann H. Asymmetric protoplast fusions between wild species and breeding lines of potato-effect of recipients and genome stability. Theor Appl Genet,1998,97: 1347-1354
104.O'Neill C, Murata T, Morgan C, Mathias R. Expression of the C3-C4 intermediate character in somatic hybrids between Brassica napus and the C3-C4 species Moricandia arvensis. Theor Appl Genet,1996,93:1234-1241
105.Parokonny A, Kenton A, Gleba Y, Bennett M. Genome reorganization in Nicotiana asymmetric somatic hybrids analysed by in situ hybridization. Plant J,1992,2: 863-874
106. Perl A, Aviv D, Galun E. Protoplast-fusion-derived CMS potato cybrids:potential seed-parents for hybrid, True-Potato-Seeds. J Heredity,1990,81:438-442
107.Piastuch WC, Bates GW. Chromosomal analysis of Nicotiana asymmetric somatic hybrids by dot blotting and in situ hybridization. Mol Gen Genet,1990,222:97-103
108. Provan J, Powell W, Waugh R. Microsatellite analysis of relationships within cultivated potato(Solanum tuberosum). Theor Appl Genet,1996,92:1078-1084
109. Schoenmakers H, Wolters A-M, Haan Ad, Saiedi A, Koornneef M. Asymmetric somatic hybridization between tomato(Lycopersicon esculentum Mill) and gamma-irradiated potato(Solanum tuberosum L.):a quantitative analysis. Theor Appl Genet,1994,87:713-720
110. Scotti N, Cozzolino S, Cardi T. Mitochondrial DNA variation in cultivated and wild potato species (Solanum spp.). Genome,2007,50:706-713
111. Scotti N, Marechal-Drouard L, Cardi T. The rpl5-rps14 mitochondrial region:a hot spot for DNA rearrangements in Solanum spp. somatic hybrids. Curr Genet,2004, 45:378-382
112.Shepard JF, Bidney D, Shahin E. Potato protoplasts in crop improvement. Science, 1980,208:17
113.Sherraf I, Tizroutine S, Chaput M, Allot M, Mussio I, Sihachakr D, Rossignol L, Ducreux G. Production and characterization of intergeneric somatic hybrids through protoplast electrofusion between potato(Solanum tuberosum) and Lycopersicon pennellii Plant Cell Tiss Org,1994,37:137-144
114. Sihachakr D, Haicour R, Chaput M-H, Barrientos E, Ducreux G, Rossignol L. Somatic hybrid plants produced by electrofusion between Solanum melongena L. and Solanum torvum Sw. Theor Appl Genet,1989,77:1-6
115. Sihachakr D, Haicour R, Serraf I, Barrientos E, Herbreteau C, Ducreux G, Rossignol L, Souvannavong V. Electrofusion for the production of somatic hybrid plants of Solanum melongena L. and Solanum khasianum C.B. Clark. Plant Sci,1988,57: 215-223
116. Skarzhinskaya M, Fahleson J, Glimelius K, Mouras A. Genome organization of Brassica napus and Lesquerella fendleri and analysis of their somatic hybrids using genomic in situ hybridization. Genome,1998,41:691-701
117. Skirvin RM, Norton M, McPheeters K. Somaclonal variation:has it proved useful for plant improvement? In II International Symposium on In Vitro Culture and Horticultural Breeding 336.1992:333-340
118. Sundberg E, Glimelius K. Effects of parental ploidy level and genetic divergence on chromosome elimination and chloroplast segregation in somatic hybrids within Brassicaceae. Theor Appl Genet,1991,83:81-88
119. Szarka B, Gonter I, Molnar-Lang M, Morocz S, Dudits D. Mixing of maize and wheat genomic DNA by somatic hybridization in regenerated sterile maize plants. Theor Appl Genet,2002,105:1-7
120. Szczerbakowa A, Boltowicz D, Lebecka R, Radomski P, Wielgat B. Characteristics of the interspecific somatic hybrids Solatium pinnatisectum (+) S. tuberosum H-8105. Acta Physiol Plant,2005,27:265-273
121. Szczerbakowa A, Tarwacka J, Oskiera M, Jakuczun H, Wielgat B. Somatic hybridization between the diploids of S. × michoacanum and S. tuberosum. Acta Physiol Plant,2010,32:867-873
122. Tamura N, Murata Y, Mukaihara T. A somatic hybrid between Solanum integrifolium and Solanum violaceum that is resistant to bacterial wilt caused by Ralstonia solanacearum. Plant Cell Rep,2002,21:353-358
123. Thanh ND, Medgyesy P. Limited chloroplast gene transfer via recombination overcomes plastomegenome incompatibility between Nicotiana tabacum and Solanum tuberosum. Plant Mol Biol,1989,12:87-93
124. Thanh ND, Pay A, Smith MA, Medgyesy P, Marton L. Intertrubal chloroplast transfer by protoplast fusion between Nicotiana tabacum and Salpiglossis sinuata. Mol Gen Genet,1988,213:186-190
125.Thieme R, Darsow U, Gavrilenko T, Dorokhov D, Tiemann H. Production of somatic hybrids between S. tuberosum L. and late blight resistant Mexican wild potato species. Euphytica,1997,97:189-200
126.Thieme R, Rakosy-Tican E, Gavrilenko T, Antonova O, Schubert J, Nachtigall M, Heimbach U, Thieme T. Novel somatic hybrids(Solanum tuberosum L.+Solanum tarnii) and their fertile BC 1 progenies express extreme resistance to potato virus Y and late blight. Theor Appl Genet,2008,116:691-700
127.Tiwari JK, Sarkar D, Pandey S, Gopal J, Kumar SR. Molecular and morphological characterization of somatic hybrids between Solanum tuberosum L. and S. etuberosum Lindl. Plant Cell Tiss Org,2010,103:175-187
128.Trabelsi S, Gargouri-Bouzid R, Vedel F, Nato A, Lakhoua L, Drira N. Somatic hybrids between potato Solanum tuberosum and wild species Solanum vernei exhibit a recombination in the plastome. Plant Cell Tiss Org,2005,83:1-11
129. Trick H, Zelcer A, Bates G. Chromosome elimination in asymmetric somatic hybrids: effect of gamma dose and time in culture. Theor Appl Genet,1994,88:965-972
130.Vardi A, Arzee-Gonen P, Frydman-Shani A, Bleichman S, Galun E. Protoplast-fusion-mediated transfer of organelles from Microcitrus into Citrus and regeneration of novel alloplasmic trees. Theor Appl Genet,1989,78:741-747
131. Varotto S, Nenz E, Lucchin M, Parrini P. Production of asymmetric somatic hybrid plants between Cichorium intybus L. and Helianthus annuus L. Theor Appl Genet, 2001,102:950-956
132.Vlahova M, Hinnisdaels S, Frulleux F, Claeys M, Atanassov A, Jacobs M. UV irradiation as a tool for obtaining asymmetric somatic hybrids between Nicotiana plumbaginifolia and Lycopersicon esculentum. Theor Appl Genet,1997,94:184-191
133. Wang J, Xiang F, Xia G. Agropyron elongatum chromatin localization on the wheat chromosomes in an introgression line. Planta,2005,221:277-286
134. Wang J, Zhao C, Liu C, Xia G, Xiang F. Introgression of Swertia mussotii gene into Bupleurum scorzonerifolium via somatic hybridization. BMC Plant Biol,2011,11: 71
135. Wang T, Niizeki M, Harada T, Ishikawa R, Qian Y, Saito K. Establishment of somatic hybrid cell lines between Zea mays L.(maize) and Triticum sect, trititrigia MacKey (trititrigia). Theor Appl Genet,1993,86:371-376
136.Wolters A, Schoenmakers H, Kamstra S, Van Eden J, Koornneef M, De Jong J. Mitotic and meiotic irregularities in somatic hybrids of Lycopersicon esculentum and Solanum tuberosum. Genome,1994,37:726-735
137. Walters A, Schoenmakers H, Koornneef M. Chloroplast and mitochondrial DNA composition of triploid and tetraploid somatic hybrids between Lycopersicon esculentum and Solanum tuberosum. Theor Appl Genet,1995,90:285-293
138. Wolters A, Schoenmakers H, Van der Meulen-Muisers J, Van der Knaap E, Derks E, Koornneef M, Zelcer A. Limited DNA elimination from the irradiated potato parent in fusion products of albino Lycopersicon esculentum and Solanum tuberosum. Theor Appl Genet,1991,83:225-232
139. Wolters AMA, Vergunst AC, van der Werff F, Koornneef M. Analysis of nuclear and organellar DNA of somatic hybrid calli and plants between Lycopersicon spp. and Nicotiana spp. Mol Gen Genet,1993,241:707-718
140.Wydra K, Beri H. Immunohistochemical changes in methyl-ester distribution of homogalacturonan and side chain composition of rhamnogalacturonan I as possible components of basal resistance in tomato inoculated with Ralstonia solanacearum. Physiol Mol Plant P,2007,70:13-24
141. Xia G, Li Z, Wang S, Xiang F, Liu J, Chen P, Liu D. Asymmetric somatic hybridization between haploid common wheat and UV-irradiated Haynaldia villosa. Plant Sci,1998,137:217-223
142. Xia G, Xiang F, Zhou A, Wang H, Chen H. Asymmetric somatic hybridization between wheat (Triticum aestivum L.) and Agropyron elongatum (Host) Nevishi. Theor Appl Genet,2003,107:299-305
143. Xia G.Progress of chromosome engineering mediated by asymmetric somatic hybridization. J Genet Genomics,2009,36:547-556
144. Xu C, Xia G, Zhi D, Xiang F, Chen H. Integration of maize nuclear and mitochondrial DNA into the wheat genome through somatic hybridization. Plant Sci, 2003,165:1001-1008
145. Xu X, Liu J, Deng X. Production and characterization of intergeneric diploid cybrids derived from symmetric fusion between Microcitrus papuana Swingle and sour orange (Citrus aurantium). Euphytica,2004,136:115-123
146. Xu XY, Hu ZY, Li JF, Liu JH, Deng XX. Asymmetric somatic hybridization between UV-irradiated Citrus unshiu and C. sinensis:regeneration and characterization of hybrid shoots. Plant Cell Rep,2007,26:1263-1273
147. Yang X, Zhang X, Jin S, Fu L, Wang L. Production and characterization of asymmetric hybrids between upland cotton Coker 201 (Gossypium hirsutum) and wild cotton (G klozschianum Anderss). Plant Cell Tiss Org,2007,89:225-235
148.Zelcer A, Aviv D, Galun E. Interspecific Transfer of Cytoplasmic Male Sterility by Fusion between Protoplasts of Normal Nicotiana sylvestris and X-Ray Irradiated Protoplasts of Male-Sterile N. tabacum. Zeitschrift fur Pflanzenphysiologie,1978,90: 397-407
149. Zhao Z, Hu T, Ge X, Du X, Ding L, Li Z. Production and characterization of intergeneric somatic hybrids between Brassica napus and Orychophragmus violaceus and their backcrossing progenies. Plant Cell Rep,2008,27:1611-1621
150. Zhou A, Xia G, Zhang X, Chen H, Hu H. Analysis of chromosomal and organellar DNA of somatic hybrids between Triticum aestiuvm and Haynaldia villosa Schur. Mol Genet Genomics,2001,265:387-393
151.Zubko MK, Zubko El, Adler K, Grimm B, Gleba YY. New CMS-associated Phenotypes in Cybrids Nicotiana tabacum L.(+Hyoscyamus niger L.). Ann Bot, 2003,92:281-288
2.蔡兴奎：原生质体融合创造抗青枯病的马铃薯新种质及其遗传分析.[博士学位论文].武汉：华中农业大学图书馆,2003
3.陈文品,吴琴生,刘大钧.小麦与多年生黑麦草原生质体的电融合及杂种愈伤组织的形成.植物学报,1992,34：284-290
4.郭文武,周长河,伊华林,邓秀新.柑橘与枳属间体细胞杂种再生及其对脚腐病抗性的评价.植物学报,2000,42：668-672
5.刘喜才,张丽娟：马铃薯种质资源描述规范和数据标准.中国农业出版社,2006
6.宋锡全,夏光敏,周爱芬,鲍雪珍,陈惠民.葡萄与柴胡科间体细胞杂交再生杂种植株.科学通报,1999,44：1832-1835
7.孙慧生.马铃薯育种学.中国农业出版社,2003
8.王槐,陈正华,夏光敏,陈惠民.植物体细胞杂交的进展.生命科学,1999,11：100-103
9.吴田.马铃薯晚疫病水平抗性相关基因STPKl, STLRPKl, Star的克隆和功能分析.[博士学位论文].武汉：华中农业大学图书馆,2009.
10.夏光敏,陈惠民.小麦与高冰草原生质体融合及再生能力的恢复.山东大学学报,自然科学版,1995,30：325-330
11.夏光敏,陈穗云,向凤宁,权太勇,陈惠民.小麦与高冰草体细胞杂种F-3-F-5代的耐盐性研究.山东农业科学,2001：12-14
12.夏光敏,王槐,陈惠民.小麦与新麦草及高冰草属间不对称体细胞杂交的植株再生.科学通报,1996,41：1423-1426
13.夏光敏,向凤宁,周爱芬,王槐,何世贤,陈惠民.小麦与高冰草属间体细胞杂交获可育杂种植株.植物学报,1999,41：349-352
14.周爱芬,夏光敏,陈惠民.普通小麦与簇毛麦不对称体细胞杂交的研究.巾国科学C辑：生命科学,1996,26：31-37
15.周传恩.利用不对称体细胞杂交技术构建普通小麦辐射杂种嵌板及其图谱的研究.[博士学位论文].济南：山东大学图书馆,2007
16.周宇波.马铃薯原生质体培养及其再生植株的遗传变异研究.[硕士学位论文].武汉：华中农业大学图书馆,2000
17. Abdullah R, Thompson J, Khush G, Kaushik R, Cocking E. Protoclonal variation in the seed progeny of plants regenerated from rice protoplasts. Plant Sci,1989,65: 97-101
18. Austin S, Baer MA, Helgeson JP. Transfer of resistance to potato leaf roll virus from Solanum brevidens into Solanum tuberosum by somatic fusion. Plant Sci,1985,39: 75-81
19. Baldev A, Gaikwad K, Kirti P, Mohapatra T, Prakash S, Chopra V. Recombination between chloroplast genomes of Trachystoma ballii and Brassica juncea following protoplast fusion. Mol Gen Genet,1998,260:357-361
20. Bamberg J, Hanneman Jr R, Palta J, Harbage J. Using disomic 4x(2EBN) potato species'germplasm via bridge species Solanum commersonii. Genome,1994,37: 866-870
21. Bastia T, Carotenuto N, Basile B, Zoina A, Cardi T. Induction of novel organelle DNA variation and transfer of resistance to frost and Verticillium wilt in Solanum tuberosum through somatic hybridization with 1EBN 51 commersonii. Euphytica, 2000,116:1-10
22. Bates G, Hasenkampf C, Contolini C, Piastuch W. Asymmetric hybridization in Nicotiana by fusion of irradiated protoplasts. Theor Appl Genet,1987,74:718-726
23. Bauer-Weston B, Keller W, Webb J, Gleddie S. Production and characterization of asymmetric somatic hybrids between Arabidopsis thaliana and Brassica napus. Theor Appl Genet,1993,86:150-158
24. Bidani A, Nouri-Ellouz O, Lakhoua L, Sihachakr D, Cheniclet C, Mahjoub A, Drira N, Gargouri-Bouzid R. Interspecific potato somatic hybrids between Solanum berthaultii and Solanum tuberosum L. showed recombinant plastome and improved tolerance to salinity. Plant Cell Tiss Org,2007,91:179-189
25. Bohman S, Wang M, Dixelius C. Arabidopsis thaliana-derived resistance against Leptosphaeria maculans in a Brassica napus genomic background. Theor Appl Genet,2002,105:498-504
26. Borgato L, Conicella C, Pisani F, Furini A. Production and characterization of arboreous and fertile Solanum melongena+ Solamum marginatum somatic hybrid plants. Planta,2007,226:961-969
27. Brewer E, Saunders J, Angle JS, Chaney R, McIntosh M. Somatic hybridization between the zinc accumulator Thlaspi caerulescens and Brassica napus. Theor Appl Genet,1999,99:761-771
28. Bryan G, McNicoll J, Ramsay G, Meyer R, De Jong W. Polymorphic simple sequence repeat markers in chloroplast genomes of Solanaceous plants. Theor Appl Genet,1999,99:859-867
29. Cardi T, D'Ambrosio E, Consoli D, Puite K, Ramulu KS. Production of somatic hybrids between frost-tolerant Solanum commersonii and S.tuberosum: characterization of hybrid plants. Theor Appl Genet,1993,87:193-200
30. Carlson PS, Smith HH, Dealing RD. Parasexual interspecific plant hybridization. Proc Natl Acad Sci,1972,69:2292-2294
31. Carputo D, Barone A, Cardi T, Sebastiano A, Frusciante L, Peloquin SJ. Endosperm balance number manipulation for direct in vivo germplasm introgression to potato from a sexually isolated relative(Solanum commersonii Dun.). Proc Natl Acad Sci, 1997,94:12013-12017
32. Cheng A, Xia G. Somatic hybridisation between common wheat and Italian ryegrass. Plant Sci,2004b,166:1219-1226
33. Cheng AX, Xia GM, Zhi DY, CHEN HM. Intermediate fertile Triticum aestivum (+) Agropyron elongatum somatic hybrids are generated by low doses of UV irradiation. Cell Res,2004a,14:86-91
34. Collonnier C, Fock I, Mariska I, Servaes A, Vedel F, Siljak-Yakovlev S, Souvannavong V, Sihachakr D. GISH confirmation of somatic hybrids between Solanum melongena and S. torvum:assessment of resistance to both fungal and bacterial wilts. Plant Physiol Biochem,2003,41:459-470
35. Collonnier C, Mulya K, Fock I, Mariska I, Servaes A, Vedel F, Siljak-Yakovlev S, Souvannavong V, Ducreux G, Sihachakr D. Source of resistance against Ralstonia solanacearum in fertile somatic hybrids of eggplant Solanum melongena with Solanum aethiopicum L. Plant Sci,2001,160:301-313
36. Cui H, Yu Z, Deng J, Gao X, Sun Y, Xia G. Introgression of bread wheat chromatin into tall wheatgrass via somatic hybridization. Planta,2009,229:323-330
37. Daunay M, Chaput M, Sihachakr D, Allot M, Vedel F, Ducreux G. Production and characterization of fertile somatic hybrids of eggplant(Solanum melongena L.) with Solanum aethiopicum L. Theor Appl Genet,1993,85:841-850
38. Demesure B, Sodzi N, Petit R. A set of universal primers for amplification of polymorphic non-coding regions of mitochondrial and chloroplast DNA in plants. Mol Ecol,1995,4:129-131
39. Deng JY, Cui HF, Zhi DY, Zhou CN, Xia GM. Analysis of remote asymmetric somatic hybrids between common wheat and Arabidopsis thaliana. Plant Cell Rep, 2007,26:1233-1241
40. Dudits D, Fejer O, Hadlaczky G, Koncz C, Lazar GB, Horvath G. Intergeneric gene transfer mediated by plant protoplast fusion. Mol Gen Genet,1980,179:283-288
41. Dumolin-Lapegue S, Pemonge M, Petit R. An enlarged set of consensus primers for the study of organelle DNA in plants. Mol Ecol,1997,6:393-397
42. Ehlenfeldt M, Helgeson J. Fertility of somatic hybrids from protoplast fusions of Solanum brevidens and & tuberosum. Theor Appl Genet,1987,73:395-402
43. Elphinstone, JG Inheritance of resistance to bacterial diseases. In:Bradshaw JE, Mackay GR eds., Potato genetics. CAB International, Wallingford,1994.429-446
44. Fahleson J, Eriksson I, Landgren M, Stymne S, Glimelius K. Intertribal somatic hybrids between Brassica napus and Thlaspi perfoliatum with high content of the T. perfoliatum-specific nervonic acid. TheorAppl Genet,1994,87:795-804
45. Fan LM, Wang YF, Wu WH. Outward K+ channels in Brassica chinensis pollen protoplasts are regulated by external and internal pH. Protoplasma,2003,220: 143-152
46. Feingold S, Lloyd J, Norero N, Bonierbale M, Lorenzen J. Mapping and characterization of new EST-derived microsatellites for potato(Solanum tuberosum L.). Theor Appl Genet,2005,111:456-466
47. Fock I, Collonnier C, Luisetti J, Purwito A, Souvannavong V, Vedel F, Servaes A, Ambroise A, Kodja H, Ducreux G, Sihachakr D. Use of Solanum stenotomum for introduction of resistance to bacterial wilt in somatic hybrids of potato. Plant Physiol Biochem,2001,39:899-908
48. Fock I, Collonnier C, Purwito A, Luisetti J, Souvannavong V, Vedel F, Servaes A, Ambroise A, Kodja H, Ducreux G, Sihachakr D. Resistance to bacterial wilt in somatic hybrids between Solanum tuberosum and Solanum phureja. Plant Sci,2000, 160:165-176
49. Forsberg J, Lagercrantz U, Glimelius K. Comparison of UV light, X-ray and restriction enzyme treatment as tools in production of asymmetric somatic hybrids between Brassica napus and Arabidopsis thaliana. Theor Appl Genet,1998,96: 1178-1185
50. Gaikwad K, Kirti P, Sharma A, Prakash S, Chopra V. Cytogenetical and molecular investigations on somatic hybrids of Sinapis alba and Brassica juncea and their backcross progeny. Plant Breeding,1996,115:480-483
51. Gavrilenko T, Thieme R, Rokka V-M. Cytogenetic analysis of Lycopersicon esculentum (+) Solanum etuberosum somatic hybrids and their androgenetic regenerants. Theor Appl Genet,2001,103:231-239
52. Gavrilenko T, Thieme R, Tiemann H. Assessment of genetic and phenotypic variation among intraspecific somatic hybrids of potato, Solanum tuberosum L. Plant Breeding,1999,118:205-213
53. Gibson R, Jones M, Fish N. Resistance to potato leaf roll virus and potato virus Y in somatic hybrids between dihaploid Solanum tuberosum and S. brevidens. Theor Appl Genet,1988,76:113-117
54. Gilissen L, Staveren Mv, Verhoeven H, Sree Ramulu K. Somatic hybridization between potato and Nicotiana plumbaginifolia. Theor Appl Genet,1992,84:73-80
55. Gleddie S, Fassuliotis G, Keller W, Setterfield G. Somatic hybridization as a potential method of transferring nematode and mite resistance into eggplant. Z Pflanzenzuchtung,1985,94:352-355
56. Gleddie S, Keller W, Setterfield G.Production and characterization of somatic hybrids between Solanum melongena L. and S. sisymbriifolium Lam. Theor Appl Genet,1986,71:613-621
57. Greplova M, Polzerova H, Vlastnikova H. Electrofusion of protoplasts from Solamum tuberosum, S. bulbocastanum and S. pinnatisectum. Acta Physiol Plant,2008,30: 787-796
58. Guo W, Deng X. Somatic hybrid plantlets regeneration between Citrus and its wild relative, Murraya paniculata via protoplast electrofusion. Plant Cell Rep,1998,18: 297-300
59. Guo XP, Xie CH, Cai XK, Song BT, He L, Liu J. Meiotic behavior of pollen mother cells in relation to ploidy level of somatic hybrids between Solanum tuberosum and S. chacoense. Plant Cell Rep,2010,29:1277-1285
60. Guri A, Sink K. Interspecific somatic hybrid plants between eggplant (Solanum melongena) and Solanum torvum. Theor Appl Genet,1988a,76:490-496
61. Guri A, Sink K. Organelle composition in somatic hybrids between an atrazine resistant biotype of Solanum nigrum and Solanum melongena. Plant Sci,1988b,58: 51-58
62. Hansen L, Earle E. Somatic hybrids between Brassica oleracea L. and Sinapis alba L. with resistance to Alternaria brassicae (Berk.) Sacc. Theor Appl Genet,1997,94: 1078-1085
63. Hayward AC. Biology and epidemiology of bacterial wilt caused by Pseudomonas solanacearum. Annu Rev Phytopathol,1991,29:65-87
64. Helgeson J, Pohlman J, Austin S, Haberlach G, Wielgus S, Ronis D, Zambolim L, Tooley P, McGrath J, James R. Somatic hybrids between Solanum bulbocastanum and potato:a new source of resistance to late blight. Theor Appl Genet,1998,96: 738-742
65. Helgeson JP, Haberlach GT:Somatic hybrids of Solanum tuberosum and related species. In Plant Biotechnology and In Vitro Biology in the 21st Century. Springer; 1999:151-154:
66. Hoffmann F, Adachi T. "Arabidobrassica":Chromosomal recombination and morphogenesis in asymmetric intergeneric hybrid cells. Planta,1981,153:586-593
67. Horsman K, Gavrilenko T, Bergervoet M, Huigen DJ, Joe A, Jacobsen E. Alteration of the genomic composition of Solanum nigrum (+) potato backcross derivatives by somatic hybridization:selection of fusion hybrids by DNA measurements and GISH. Plant Breeding,2001,120:201-207
68. Hu Q, Hansen L, Laursen J, Dixelius C, Andersen S. Intergeneric hybrids between Brassica napus and Orychophragmus violaceus containing traits of agronomic importance for oilseed rape breeding. Theor Appl Genet,2002,105:834-840
69. Ishikawa S, Bang S, Kaneko Y, Matsuzawa Y, Robbelen G. Production and characterization of intergeneric somatic hybrids between Moricandia arvensis and Brassica oleracea. Plant Breeding,2003,122:233-238
70. Jarl C, Rietveld E, De Haas J. Transfer of fungal tolerance through interspecific somatic hybridisation between Solanum melongena and S. torvum. Plant Cell Rep, 1999,18:791-796
71. Jelenic S, Berljak J, Papes D, Jelaska S. Mixoploidy and chimeric structures in somaclones of potato (Solanum tuberosum L.) cv. Bintje. Food Technol Biotech, 2001,39:13-18
72. Johnston S, Hanneman R. Manipulations of endosperm balance number overcome crossing barriers between diploid Solanum species. Science,1982,217:446-448
73. Kanno A, Kanzaki H, Kameya T. Detailed analyses of chloroplast and mitochondrial DNAs from the hybrid plant generated by asymmetric protoplast fusion between radish and cabbage. Plant Cell Rep,1997,16:479-484
74. Kao KN, Michayluk MR. Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta,1975, 126:105-110
75. Kim H, Chae M, Choi S, Wielgus S, Helgeson J. Identification of somatic hybrids produced by protoplast fusion between Solanum commersonii and S. tuberosum haploid. Korean J Plant Tissue Cult,1993,20:337-344
76. Kim YJ, Kim JE, Lee J-H, Lee MH, Jung HW, Bahk YY, Hwang BK, Hwang I, Kim WT. The Vr-PLC3 gene encodes a putative plasma membrane-localized phosphoinositide-specific phospholipase C whose expression is induced by abiotic stress in mung bean (Vigna radiata L.). FEBS letters,2004,556:127-136
77. Kim-Lee H, Moon J, Hong Y, Kim M, Cho H. Bacterial wilt resistance in the progenies of the fusion hybrids between haploid of potato and Solanum commersonii. Am J Potato Res,2005,82:129-137
78. Kisaka H, Kisaka M, Kanno A, Kameya T. Intergeneric somatic hybridization of rice (Oryza sativa L.) and barley (Hordeum vulgare L.) by protoplast fusion. Plant Cell Rep,1998,17:362-367
79. Kisaka H, Kisaka M, Kanno A, Kameya T. Production and analysis of plants that are somatic hybrids of barley (Hordeum vulgare L.) and carrot (Daucus carota L.). Theor Appl Genet,1997,94:221-226
80. Kostenyuk I, Lubaretz O, Borisyuk N, Voronin V, Stockigt J, Gleba Y. Isolation and characterization of intergeneric somatic hybrids in the Apocynaceae family. Theor Appl Genet,1991,82:713-716
81. Kyozuka J, Kaneda T, Shimamoto K. Production of cytoplasmic male sterile rice (Oryza sativa L.) by cell fusion. Nat Biotechnol,1989,7:1171-1174
82. Laferriere L, Helgeson J, Allen C. Fertile Solanum tuberosum+ S. commersonii somatic hybrids as sources of resistance to bacterial wilt caused by Ralstonia solanacearum. Theor Appl Genet,1999,98:1272-1278
83. Larkin PJ, Scowcroft W. Somaclonal variation-a novel source of variability from cell cultures for plant improvement. Theor Appl Genet,1981,60:197-214
84. Lee SH, Shon YQ Kim CY, Chun HJ, Cheong YH, Kim ZH, Choe ZR, Choi YJ, Cho MJ. Variations in the morphology of rice plants regenerated from protoplasts using different culture procedures. Plant Cell Tiss Org,1999,57:179-187
85. Lelivelt C, Krens F. Transfer of resistance to the beet cyst nematode (Heterodera schachtii Schm.) into the Brassica napus L. gene pool through intergeneric somatic hybridization with Raphanus sativus L. Theor Appl Genet,1992,83:887-894
86. Liang X, Ding S, Wong S. Development of a kenaf (Hibiscus cannabinus L.) protoplast system for a replication study of Hibiscus chlorotic ringspot virus. Plant Cell Rep,2002,20:982-986
87. Liu J, Xu X, Deng X. Intergeneric somatic hybridization and its application to crop genetic improvement. Plant Cell Tiss Org,2005,82:19-44
88. Liu JH, Hu CG, Deng XX. Regeneration of diploid intergeneric somatic hybrid plants between Microcitrus and Citrus via electrofusion. Acta Bot Sin,1999,41:1177-1182
89. Liu JH, Pang XM, Cheng YJ, Meng HJ, Deng X.Molecular characterization of the nuclear and cytoplasmic genomes of intergeneric diploid plants from cell fusion between Microcitrus papuana and Rough lemon. Plant Cell Rep,2002,21:327-332
90. Lossl A, Adler N, Horn R, Frei U, Wenzel G. Chondriome-type characterization of potato:mt α, β, γδε and novel plastid-mitochondrial configurations in somatic hybrids. Theor Appl Genet,1999,99:1-10
91. Melzer J, O'Connell M. Effect of radiation dose on the production of and the extent of asymmetry in tomato asymmetric somatic hybrids. Theor Appl Genet,1992,83: 337-344
92. Menke U, Schilde-Rentschler L, Ruoss B, Zanke C, Hemleben V, Ninnemann H. Somatic hybrids between the cultivated potato Solanum tuberosum L. and the 1EBN wild species Solanum pinnatisectum Dun.:morphological and molecular characterization. Theor Appl Genet,1996,92:617-626
93. Mezencev N, Clement G, Guiderdoni E. Variation among progenies of diploid plants regenerated from haploid, microspore-derived cell-suspension protoplasts of rice (Oryza sativa L.). Plant Breeding,1995,114:149-154
94. Milbourne D, Meyer R, Collins A, Ramsay L, Gebhardt C, Waugh R. Isolation, characterisation and mapping of simple sequence repeat loci in potato. Mol Gen Genet,1998,259:233-245
95. Mliki A, Jardak R, Reustle G, Ghorbel A. Isolation and culture of leaf protoplasts from Tunisian grapes. JInt Sci Vigne Vin,2003,37:145-153
96. Mohapatra T, Kirti P, Kumar VD, Prakash S, Chopra V. Random chloroplast segregation and mitochondrial genome recombination in somatic hybrid plants of Diplotaxis catholica+ Brassica juncea. Plant Cell Rep,1998,17:814-818
97. Moreira CD, Chase CD, Gmitter Jr FG, Grosser JW. Inheritance of organelle genomes in citrus somatic cybrids. Mol Breeding,2000,6:401-405
98. Nouri-Ellouz O, Gargouri-Bouzid R, Sihachakr D, Triki MA, Ducreux G, Drira N, Lakhoua L. Production of potato intraspecific somatic hybrids with improved tolerance to PVY and Pythium aphanidermatum. J Plant Physiol,2006,163: 1321-1332
99. Novy R, Helgeson J. Resistance to potato virus Y in somatic hybrids between Solanum etuberosum and S. tuberosum x S. berthaultii hybrid. Theor Appl Genet, 1994a,89:783-786
100. Novy R, Helgeson J. Somatic hybrids between Solanum etuberosum and diploid, tuber bearing Solanum clones. Theor Appl Genet,1994b,89:775-782
101.Nunome T, Negoro S, Kono I, Kanamori H, Miyatake K, Yamaguchi H, Ohyatna A, Fukuoka H. Development of SSR markers derived from SSR-enriched genomic library of eggplant (Solanum melongena L.). Theor Appl Genet,2009,119: 1143-1153
102.Nyman M, Waara S. Characterisation of somatic hybrids between Solanum tuberosum and its frost-tolerant relative Solanum commersonil. Theor Appl Genet, 1997,95:1127-1132
103.Oberwalder B, Schilde-Rentschler L, Ruofβ B, Wittemann S, Ninnemann H. Asymmetric protoplast fusions between wild species and breeding lines of potato-effect of recipients and genome stability. Theor Appl Genet,1998,97: 1347-1354
104.O'Neill C, Murata T, Morgan C, Mathias R. Expression of the C3-C4 intermediate character in somatic hybrids between Brassica napus and the C3-C4 species Moricandia arvensis. Theor Appl Genet,1996,93:1234-1241
105.Parokonny A, Kenton A, Gleba Y, Bennett M. Genome reorganization in Nicotiana asymmetric somatic hybrids analysed by in situ hybridization. Plant J,1992,2: 863-874
106. Perl A, Aviv D, Galun E. Protoplast-fusion-derived CMS potato cybrids:potential seed-parents for hybrid, True-Potato-Seeds. J Heredity,1990,81:438-442
107.Piastuch WC, Bates GW. Chromosomal analysis of Nicotiana asymmetric somatic hybrids by dot blotting and in situ hybridization. Mol Gen Genet,1990,222:97-103
108. Provan J, Powell W, Waugh R. Microsatellite analysis of relationships within cultivated potato(Solanum tuberosum). Theor Appl Genet,1996,92:1078-1084
109. Schoenmakers H, Wolters A-M, Haan Ad, Saiedi A, Koornneef M. Asymmetric somatic hybridization between tomato(Lycopersicon esculentum Mill) and gamma-irradiated potato(Solanum tuberosum L.):a quantitative analysis. Theor Appl Genet,1994,87:713-720
110. Scotti N, Cozzolino S, Cardi T. Mitochondrial DNA variation in cultivated and wild potato species (Solanum spp.). Genome,2007,50:706-713
111. Scotti N, Marechal-Drouard L, Cardi T. The rpl5-rps14 mitochondrial region:a hot spot for DNA rearrangements in Solanum spp. somatic hybrids. Curr Genet,2004, 45:378-382
112.Shepard JF, Bidney D, Shahin E. Potato protoplasts in crop improvement. Science, 1980,208:17
113.Sherraf I, Tizroutine S, Chaput M, Allot M, Mussio I, Sihachakr D, Rossignol L, Ducreux G. Production and characterization of intergeneric somatic hybrids through protoplast electrofusion between potato(Solanum tuberosum) and Lycopersicon pennellii Plant Cell Tiss Org,1994,37:137-144
114. Sihachakr D, Haicour R, Chaput M-H, Barrientos E, Ducreux G, Rossignol L. Somatic hybrid plants produced by electrofusion between Solanum melongena L. and Solanum torvum Sw. Theor Appl Genet,1989,77:1-6
115. Sihachakr D, Haicour R, Serraf I, Barrientos E, Herbreteau C, Ducreux G, Rossignol L, Souvannavong V. Electrofusion for the production of somatic hybrid plants of Solanum melongena L. and Solanum khasianum C.B. Clark. Plant Sci,1988,57: 215-223
116. Skarzhinskaya M, Fahleson J, Glimelius K, Mouras A. Genome organization of Brassica napus and Lesquerella fendleri and analysis of their somatic hybrids using genomic in situ hybridization. Genome,1998,41:691-701
117. Skirvin RM, Norton M, McPheeters K. Somaclonal variation:has it proved useful for plant improvement? In II International Symposium on In Vitro Culture and Horticultural Breeding 336.1992:333-340
118. Sundberg E, Glimelius K. Effects of parental ploidy level and genetic divergence on chromosome elimination and chloroplast segregation in somatic hybrids within Brassicaceae. Theor Appl Genet,1991,83:81-88
119. Szarka B, Gonter I, Molnar-Lang M, Morocz S, Dudits D. Mixing of maize and wheat genomic DNA by somatic hybridization in regenerated sterile maize plants. Theor Appl Genet,2002,105:1-7
120. Szczerbakowa A, Boltowicz D, Lebecka R, Radomski P, Wielgat B. Characteristics of the interspecific somatic hybrids Solatium pinnatisectum (+) S. tuberosum H-8105. Acta Physiol Plant,2005,27:265-273
121. Szczerbakowa A, Tarwacka J, Oskiera M, Jakuczun H, Wielgat B. Somatic hybridization between the diploids of S. × michoacanum and S. tuberosum. Acta Physiol Plant,2010,32:867-873
122. Tamura N, Murata Y, Mukaihara T. A somatic hybrid between Solanum integrifolium and Solanum violaceum that is resistant to bacterial wilt caused by Ralstonia solanacearum. Plant Cell Rep,2002,21:353-358
123. Thanh ND, Medgyesy P. Limited chloroplast gene transfer via recombination overcomes plastomegenome incompatibility between Nicotiana tabacum and Solanum tuberosum. Plant Mol Biol,1989,12:87-93
124. Thanh ND, Pay A, Smith MA, Medgyesy P, Marton L. Intertrubal chloroplast transfer by protoplast fusion between Nicotiana tabacum and Salpiglossis sinuata. Mol Gen Genet,1988,213:186-190
125.Thieme R, Darsow U, Gavrilenko T, Dorokhov D, Tiemann H. Production of somatic hybrids between S. tuberosum L. and late blight resistant Mexican wild potato species. Euphytica,1997,97:189-200
126.Thieme R, Rakosy-Tican E, Gavrilenko T, Antonova O, Schubert J, Nachtigall M, Heimbach U, Thieme T. Novel somatic hybrids(Solanum tuberosum L.+Solanum tarnii) and their fertile BC 1 progenies express extreme resistance to potato virus Y and late blight. Theor Appl Genet,2008,116:691-700
127.Tiwari JK, Sarkar D, Pandey S, Gopal J, Kumar SR. Molecular and morphological characterization of somatic hybrids between Solanum tuberosum L. and S. etuberosum Lindl. Plant Cell Tiss Org,2010,103:175-187
128.Trabelsi S, Gargouri-Bouzid R, Vedel F, Nato A, Lakhoua L, Drira N. Somatic hybrids between potato Solanum tuberosum and wild species Solanum vernei exhibit a recombination in the plastome. Plant Cell Tiss Org,2005,83:1-11
129. Trick H, Zelcer A, Bates G. Chromosome elimination in asymmetric somatic hybrids: effect of gamma dose and time in culture. Theor Appl Genet,1994,88:965-972
130.Vardi A, Arzee-Gonen P, Frydman-Shani A, Bleichman S, Galun E. Protoplast-fusion-mediated transfer of organelles from Microcitrus into Citrus and regeneration of novel alloplasmic trees. Theor Appl Genet,1989,78:741-747
131. Varotto S, Nenz E, Lucchin M, Parrini P. Production of asymmetric somatic hybrid plants between Cichorium intybus L. and Helianthus annuus L. Theor Appl Genet, 2001,102:950-956
132.Vlahova M, Hinnisdaels S, Frulleux F, Claeys M, Atanassov A, Jacobs M. UV irradiation as a tool for obtaining asymmetric somatic hybrids between Nicotiana plumbaginifolia and Lycopersicon esculentum. Theor Appl Genet,1997,94:184-191
133. Wang J, Xiang F, Xia G. Agropyron elongatum chromatin localization on the wheat chromosomes in an introgression line. Planta,2005,221:277-286
134. Wang J, Zhao C, Liu C, Xia G, Xiang F. Introgression of Swertia mussotii gene into Bupleurum scorzonerifolium via somatic hybridization. BMC Plant Biol,2011,11: 71
135. Wang T, Niizeki M, Harada T, Ishikawa R, Qian Y, Saito K. Establishment of somatic hybrid cell lines between Zea mays L.(maize) and Triticum sect, trititrigia MacKey (trititrigia). Theor Appl Genet,1993,86:371-376
136.Wolters A, Schoenmakers H, Kamstra S, Van Eden J, Koornneef M, De Jong J. Mitotic and meiotic irregularities in somatic hybrids of Lycopersicon esculentum and Solanum tuberosum. Genome,1994,37:726-735
137. Walters A, Schoenmakers H, Koornneef M. Chloroplast and mitochondrial DNA composition of triploid and tetraploid somatic hybrids between Lycopersicon esculentum and Solanum tuberosum. Theor Appl Genet,1995,90:285-293
138. Wolters A, Schoenmakers H, Van der Meulen-Muisers J, Van der Knaap E, Derks E, Koornneef M, Zelcer A. Limited DNA elimination from the irradiated potato parent in fusion products of albino Lycopersicon esculentum and Solanum tuberosum. Theor Appl Genet,1991,83:225-232
139. Wolters AMA, Vergunst AC, van der Werff F, Koornneef M. Analysis of nuclear and organellar DNA of somatic hybrid calli and plants between Lycopersicon spp. and Nicotiana spp. Mol Gen Genet,1993,241:707-718
140.Wydra K, Beri H. Immunohistochemical changes in methyl-ester distribution of homogalacturonan and side chain composition of rhamnogalacturonan I as possible components of basal resistance in tomato inoculated with Ralstonia solanacearum. Physiol Mol Plant P,2007,70:13-24
141. Xia G, Li Z, Wang S, Xiang F, Liu J, Chen P, Liu D. Asymmetric somatic hybridization between haploid common wheat and UV-irradiated Haynaldia villosa. Plant Sci,1998,137:217-223
142. Xia G, Xiang F, Zhou A, Wang H, Chen H. Asymmetric somatic hybridization between wheat (Triticum aestivum L.) and Agropyron elongatum (Host) Nevishi. Theor Appl Genet,2003,107:299-305
143. Xia G.Progress of chromosome engineering mediated by asymmetric somatic hybridization. J Genet Genomics,2009,36:547-556
144. Xu C, Xia G, Zhi D, Xiang F, Chen H. Integration of maize nuclear and mitochondrial DNA into the wheat genome through somatic hybridization. Plant Sci, 2003,165:1001-1008
145. Xu X, Liu J, Deng X. Production and characterization of intergeneric diploid cybrids derived from symmetric fusion between Microcitrus papuana Swingle and sour orange (Citrus aurantium). Euphytica,2004,136:115-123
146. Xu XY, Hu ZY, Li JF, Liu JH, Deng XX. Asymmetric somatic hybridization between UV-irradiated Citrus unshiu and C. sinensis:regeneration and characterization of hybrid shoots. Plant Cell Rep,2007,26:1263-1273
147. Yang X, Zhang X, Jin S, Fu L, Wang L. Production and characterization of asymmetric hybrids between upland cotton Coker 201 (Gossypium hirsutum) and wild cotton (G klozschianum Anderss). Plant Cell Tiss Org,2007,89:225-235
148.Zelcer A, Aviv D, Galun E. Interspecific Transfer of Cytoplasmic Male Sterility by Fusion between Protoplasts of Normal Nicotiana sylvestris and X-Ray Irradiated Protoplasts of Male-Sterile N. tabacum. Zeitschrift fur Pflanzenphysiologie,1978,90: 397-407
149. Zhao Z, Hu T, Ge X, Du X, Ding L, Li Z. Production and characterization of intergeneric somatic hybrids between Brassica napus and Orychophragmus violaceus and their backcrossing progenies. Plant Cell Rep,2008,27:1611-1621
150. Zhou A, Xia G, Zhang X, Chen H, Hu H. Analysis of chromosomal and organellar DNA of somatic hybrids between Triticum aestiuvm and Haynaldia villosa Schur. Mol Genet Genomics,2001,265:387-393
151.Zubko MK, Zubko El, Adler K, Grimm B, Gleba YY. New CMS-associated Phenotypes in Cybrids Nicotiana tabacum L.(+Hyoscyamus niger L.). Ann Bot, 2003,92:281-288
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |