三种菊科植物及一种瑞香科植物化学成分研究
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摘要
目的:第一部分,菊科橐吾属Ligularia Cass.植物棉毛橐吾Ligulariavellerea在民间用于治疗肝炎,为了探究棉毛橐吾中的功效物质基础,曾有人从棉毛橐吾中分离鉴定出20个化合物,为了更深入地研究棉毛橐吾中的活性成分,本文再次对该植物进行了化学成分研究;第二部分,离舌橐吾生长在海拔1400-3300米的河边、山坡及树林里,主要分布我国的云南西北部、四川、贵州、湖北西部、甘肃西南部和陕西南部,在民间主要作治疗流行性感冒、咳嗽、溃疡和肺结核等症,至今为止,文献记载已从离舌橐吾中分离得到多个艾里莫芬烷类倍半萜化合物,我们对采自河南省伏牛山系的该植物(本山系的该植物未有人研究过)进行了再研究,拟从中分离得到不同的活性单体成分;第三部分,两似蟹甲草为菊科蟹甲草属植物,蟹甲草属植物广泛分布于亚洲东北部及美洲,该属大约50种分布在中国,其中26种作为传统药物用于治疗支气管炎、关节炎及咳血等症,我们系统查阅了美国化学文摘,未发现对两似蟹甲草的化学成分的任何研究,而蟹甲草属与橐吾属在植物分类学上有很近的亲缘关系,蟹甲草属很多植物含有丰富的艾里莫芬烷型倍半萜,于是为了研究该植物可能的物质基础及丰富植物化学分类学的内容,我们对其进行了化学成分的研究;芫花为常用中药,为瑞香科瑞香属植物芫花Daphne genkwa Sieb.et Zucc.的花,性辛、温,有毒,有泻水逐饮、解毒之功效,但该药材的质量标准研究迄今还没有,所以我们对芫花的化学成分进行了系统研究,作为芫花药材质量标准研究的一部分内容。
方法:应用硅胶、Sephadex LH-20、MCI gel HP-40P、Toyopearl HW 40(c)、反相硅胶等多种分离技术,对以上四种植物中的化学成分进行分离,并利用现代波谱技术包括~1H NMR、~(13)C NMR、2D NMR及MS等对单体化合物进行结构鉴定,采用MTT法对离舌橐吾中得到的部分化合物进行了抗肿瘤活性的初步筛选。
结果:共从四种植物中分离出51个化合物,从棉毛橐吾中分离鉴定出27个单体化合物:1,5-二咖啡酰奎宁酸(Ⅰ-1),3,5-二咖啡酰奎宁酸(Ⅰ-2),艾里莫芬-7(11)-烯-12,8α(14β,6α)-二内酯(Ⅰ-3),对羟基苯乙酮(Ⅰ-4),7-羟基香豆素(Ⅰ-5),3,4-二羟基苯乙酮(Ⅰ-6),4-甲氧基-2',4',3-三羟基-查耳酮(Ⅰ-7),2',4',4-三羟基-查耳酮(Ⅰ-8),4'-甲氧基-7,3'-二羟基黄酮(Ⅰ-9),4-[(3,4-二羟基)-桂皮酰基]-桂皮酰基甲酯(Ⅰ-10),对羟基桂皮酸甲酯(Ⅰ-11a),对羟基桂皮酸(Ⅰ-11b),Ⅰ-12 2,5-二羟基-6,7-二甲基-萘醌(Ⅰ-12),4-O-咖啡酰奎宁酸(Ⅰ-13),3,4-二羟基桂皮酸(Ⅰ-14),6,7-二羟基香豆素(Ⅰ-15),3-O-咖啡酰奎宁酸(Ⅰ-16),8β-甲氧基埃里莫芬-7(11)-烯-12,8α(14β,6α)-二内酯(Ⅰ-17),雅槛蓝型艾里莫芬-14β,6α-内酯(Ⅰ-18),顺芷酸(Ⅰ-19),β-谷甾醇(Ⅰ-20),8β-羟基艾里莫芬-7(11)-烯-12,8α(14β,6α)-二内酯(Ⅰ-21),1-甘油醇十六碳酸酯(Ⅰ-22),胡萝卜苷(Ⅰ-23),十六碳酸(Ⅰ-24),十九碳酸甲酯(Ⅰ-25),艾里莫芬-7(11),9-二烯-12,8α(14β,6α)-二内酯(Ⅰ-26);从离舌橐吾中分离鉴定了10个化合物:1,3-二咖啡酰奎宁酸(Ⅱ-1),1,5-二咖啡酰奎宁酸(Ⅱ-2),艾里莫芬烷-6-烯-11-醇(Ⅱ-3),β-谷甾醇(Ⅱ-4),艾里莫芬-6α-羟基-8α,12-内酯(Ⅱ-5),10β-氢-6β,8β-二羟基艾里莫芬-8α,12 -内酯(Ⅱ-6),10α-氢-6α,8α-二羟基艾里莫芬-8β,12 -内酯(Ⅱ-6a),艾里莫芬烷-1(10)-烯-3-氧-11-醇(Ⅱ-7),(7α,9α,10α)-9α,10α-环氧-艾里莫芬烷-11-醇(Ⅱ-8),10α,11-内过氧桥-1(2)-烯-7α-氢艾里莫芬烷(Ⅱ-9);从两似蟹甲草中分离鉴定了4个化合物,包括两个对映贝壳杉烷二萜(Ⅲ-2和3),β-谷甾醇(Ⅲ-1)及1个甘油醇酯(Ⅲ-4);从芫花中分离得到10个化合物:异西瑞香素(Ⅳ-1),5,7,3',4'-四羟基黄酮(Ⅳ-2),5,7,4'-三羟基黄酮(Ⅳ-3),5,3',4'-三羟基黄酮(Ⅳ-4),4',7-二甲氧基-5-羟基黄酮(Ⅳ-5),二十八碳烷(Ⅳ-6),三十二碳烷(Ⅳ-7),4S,7S-金色酰胺醇酯(Ⅳ-8),7-甲氧基-4',5-二羟基黄酮(Ⅳ-9),β-谷甾醇(Ⅳ-10)。
结论:从棉毛橐吾中分离鉴定的27个化合物中,2个为新化合物(Ⅰ-10和12),首次报道18个;从离舌橐吾中分离鉴定的10个化合物中,新化合物4个(Ⅱ-3,6a,8,9)及1个新天然产物(Ⅱ-7),除了Ⅱ-4外其它化合物都是首
次从该植物中得到;从两似蟹甲草中分离得到的4个化合物,都为首次从两似蟹甲草中得到;从芫花中分离得到10个化合物,其中2个化合物(Ⅳ-6,7)为首次报道,并利用二维核磁谱对化合物Ⅳ-8的~1HNMR和~(13)CNMR数据进行了全部归属,对离舌橐吾中部分化合物活性测试,结果发现化合物Ⅱ-3对肺癌细胞(A849)有较强的抑制作用(IC_(50)值1.93μg/mL)。
Objective:The first part,Ligularia vellerea(Ligularia genus of Compositae) was used to deal with hepatitis as a folk medicine.For studying the material foundation of Ligularia vellerea,someone had isolated and identified 20 compounds from it.It was reinvestigated for more knowledge.The second part,the plant Ligularia veitchiana grows aside the rivers,hillsides,woods in the elevation.The plant was disributed in the northemwest of Yunnan,Sichun,Guizhou,the west of Hubei,the southernwest of Gansu,the south of Shanxi.It was used to deal with the diseases such as influenza,cough,ulcer and pulmonary tuberculosis as a folk medicine.Up to now,Many eremophilane-type sesquiterpenes were isolated from the plants.We reinvestigated it from Funiu Mountains of Henan Province(the plant of the Mountains was not studied),to plan to isolate different active compounds.The third part,Cacalia ambigua belongs to Cacalia genus(Compositae).The Cacalia genus was distributed in easternnorth of Asia and America.50 species of the genus were grown in China,among them,26 species were used to cure tracheitis,arthritis and cough and so on.We systematically consult the Scifinder data and find no results about the chemical research of Cacalia ambigua.The two genus of Cacalia and Ligularia have kinsfolk relationship on plant taxalogy.Many plants of Cacalia genus have a number of eremophilane-type sesquiterpenoids.To search for the material foundation of this plant and enlarge the knowledge of plant chemotaxology,we studied the chemical constituents.The fourth part,the flowers of Daphne genkwa Sieb.et Zucc. (Thymelaeaceae) were used to detoxify and to release the unneccessary water of body as the common traditional chinese medicine.The quality standard of this medicine has not been worked out.So we studied on the chemical constituents that could be part of study on medicinal quality standard of yuanhua.
Methods:We used all kinds of modern chromatographic techniques including silica gel,Sephadex LH-20,MCI gel CHP-40P,Toyopearl HW-40(C) and RP-18 and so on to isolate the chemical constituents of the four plants,and then we identified the compounds and assigned the spectral data of them by modern spectroscopic techniques including ~1H NMR,~(13)C NMR,2D NMR and MS.Anticancer activity of several compounds from Ligularia veitchiana were screened by MTT.
Results:51 compounds were isolated from these four plants.Among them,27 compounds from Ligularia vellerea were isolated and determined:1, 5-dicaffeoylquinic acid(Ⅰ-1),3,5-dicaffeoylquinic acid(Ⅰ-2),eremophil-7(11)-en-12, 8α(14β,6α) -dioxide(Ⅰ-3),p- hydroxyacetophenone(Ⅰ-4),7-hydroxycoumarin(Ⅰ-5),3, 4- dihydroxyacetophenone(Ⅰ-6),4-methoxy-2',4',3-trihydroxy-chalone (butein-4-methyl ester)(Ⅰ-7),2',4',4-trihydroxy-chalone(Ⅰ-8),4'-methoxy-7, 3'-dihydroxyflavone(Ⅰ-9),4-[(3,4-dihydroxyl)-cinnamyl]-cinnamyl methyl ester(Ⅰ-10),p-hydroxycoumaric acid methyl ester(Ⅰ-11a),p-hydroxycoumaric acid(Ⅰ-11b),2,5-dihydroxyl-6,7-dimethyl-naphthoquinone(Ⅰ-12),4-O-caffeoylquinic acid(Ⅰ-13),3,4-dihydroxycoumaric acid(Ⅰ-14),6,7-dihydroxycoumarin(Ⅰ-15), 3-O-caffeoylquinic acid(Ⅰ-16),8β-methoxyeremophil-7(11)-en-12,8α(14β,6α) -dioxide(Ⅰ-17),furoeremophil-15β,6α-oxide(Ⅰ-18),tiglic acid(Ⅰ-19),β-sitosterol (Ⅰ-20),8β-hydroxyeremophil-7(11)-en-12,8α(14β,6α) -dioxide(Ⅰ-21),1-glycerol palmitic acid ester(Ⅰ-22),Ⅰ-23 Daucosterol(I-23),Palmitic acid(Ⅰ-24),1-25 Nonadecanoic acid methyl ester(Ⅰ-25),eremophil-7(11),8-dien-12,8α(14β,6α) -dioxide(Ⅰ-26).
10 compounds from Ligularia veitchiana were isolated and determined:1,3-dicaffeoylquinic acid(Ⅱ-1),1,5- dicaffeoylquinic acid(Ⅱ-2), eremophilan-6-en-11-ol(Ⅱ-3),β-sitosterol(Ⅱ-4),eremophil-6α-hydroxy-8α,12-olide (Ⅱ-5),10β-H-6β,8β-dihydroxyeremophil-8α,12 -oxide(Ⅱ-6),10α-H-6α, 8α-dihydroxyeremophil-8β,12 -oxide(Ⅱ-6a),1(10)-en-3-oxoeremophilan-11-ol(Ⅱ-7), (7α,9α,10α)-9,10-epoxy- eremophilan-11-ol(Ⅱ-8), 10α,11-endo-peroxide-1(2)-en-7α-hydroeremophilane(Ⅱ-9).
4 compounds from Cacalia ambigua were isolated,ingcluding 2 ent-kaurane-type diterpenes(ent-kaur-16-en-19-oic acid(Ⅲ-2) and 13,β-kanr-16β-ol (Ⅲ-3)),β-sitosterol(Ⅲ-1) and 1 glycerol ester(1-glycerol cerotic acid ester,Ⅲ-4 ),
10 compounds from Daphne genkawa were isolated:daphnoretin(Ⅳ-1),5,7,3', 4'-tetrahydroxyflavonone(Ⅳ-2),5,7,4'-trihydroxyflavonone(Ⅳ-3),5,3', 4'-hydroxyflavonone(Ⅳ-4),4',7-dimethoxy-5-hydroxyflavonone(Ⅳ-5), Octacosane(Ⅳ-6),Dotriacontane(Ⅳ-7),4S,7S-aurantiamide acetate(Ⅳ-8), 7-methoxy-4',5-dihydroxyflavonone(Ⅳ-9),β-sitosterol(Ⅳ-10).
Conclusions:Among 27 compounds from Ligularia vellerea,2 of them were new(Ⅰ-10 and 12),18 of them were isolated from the plant for the first time.Among 10 compounds from Ligularia veitchiana,4 of them were new(Ⅱ-3,6a,8,9) and one(Ⅱ-7) was natural product,others except forⅡ-4 were isolated from the plant for the first time.4 compounds from Cacalia ambigua were isolated for the first time. Among 10 compounds from Daphne genkawa,2 of them(Ⅳ-6 and 7) were isolated from the plant for the first time.~1H NMR and ~(13)C NMR data ofⅣ- 8 were assigned by 2D NMR.The results of the bioactivity test for the several compounds from Ligularia veitchiana showed thatⅡ-3 can restrain the lung cancer cells(A849)(IC_(50) value 1.93μg/mL).
方法:应用硅胶、Sephadex LH-20、MCI gel HP-40P、Toyopearl HW 40(c)、反相硅胶等多种分离技术,对以上四种植物中的化学成分进行分离,并利用现代波谱技术包括~1H NMR、~(13)C NMR、2D NMR及MS等对单体化合物进行结构鉴定,采用MTT法对离舌橐吾中得到的部分化合物进行了抗肿瘤活性的初步筛选。
结果:共从四种植物中分离出51个化合物,从棉毛橐吾中分离鉴定出27个单体化合物:1,5-二咖啡酰奎宁酸(Ⅰ-1),3,5-二咖啡酰奎宁酸(Ⅰ-2),艾里莫芬-7(11)-烯-12,8α(14β,6α)-二内酯(Ⅰ-3),对羟基苯乙酮(Ⅰ-4),7-羟基香豆素(Ⅰ-5),3,4-二羟基苯乙酮(Ⅰ-6),4-甲氧基-2',4',3-三羟基-查耳酮(Ⅰ-7),2',4',4-三羟基-查耳酮(Ⅰ-8),4'-甲氧基-7,3'-二羟基黄酮(Ⅰ-9),4-[(3,4-二羟基)-桂皮酰基]-桂皮酰基甲酯(Ⅰ-10),对羟基桂皮酸甲酯(Ⅰ-11a),对羟基桂皮酸(Ⅰ-11b),Ⅰ-12 2,5-二羟基-6,7-二甲基-萘醌(Ⅰ-12),4-O-咖啡酰奎宁酸(Ⅰ-13),3,4-二羟基桂皮酸(Ⅰ-14),6,7-二羟基香豆素(Ⅰ-15),3-O-咖啡酰奎宁酸(Ⅰ-16),8β-甲氧基埃里莫芬-7(11)-烯-12,8α(14β,6α)-二内酯(Ⅰ-17),雅槛蓝型艾里莫芬-14β,6α-内酯(Ⅰ-18),顺芷酸(Ⅰ-19),β-谷甾醇(Ⅰ-20),8β-羟基艾里莫芬-7(11)-烯-12,8α(14β,6α)-二内酯(Ⅰ-21),1-甘油醇十六碳酸酯(Ⅰ-22),胡萝卜苷(Ⅰ-23),十六碳酸(Ⅰ-24),十九碳酸甲酯(Ⅰ-25),艾里莫芬-7(11),9-二烯-12,8α(14β,6α)-二内酯(Ⅰ-26);从离舌橐吾中分离鉴定了10个化合物:1,3-二咖啡酰奎宁酸(Ⅱ-1),1,5-二咖啡酰奎宁酸(Ⅱ-2),艾里莫芬烷-6-烯-11-醇(Ⅱ-3),β-谷甾醇(Ⅱ-4),艾里莫芬-6α-羟基-8α,12-内酯(Ⅱ-5),10β-氢-6β,8β-二羟基艾里莫芬-8α,12 -内酯(Ⅱ-6),10α-氢-6α,8α-二羟基艾里莫芬-8β,12 -内酯(Ⅱ-6a),艾里莫芬烷-1(10)-烯-3-氧-11-醇(Ⅱ-7),(7α,9α,10α)-9α,10α-环氧-艾里莫芬烷-11-醇(Ⅱ-8),10α,11-内过氧桥-1(2)-烯-7α-氢艾里莫芬烷(Ⅱ-9);从两似蟹甲草中分离鉴定了4个化合物,包括两个对映贝壳杉烷二萜(Ⅲ-2和3),β-谷甾醇(Ⅲ-1)及1个甘油醇酯(Ⅲ-4);从芫花中分离得到10个化合物:异西瑞香素(Ⅳ-1),5,7,3',4'-四羟基黄酮(Ⅳ-2),5,7,4'-三羟基黄酮(Ⅳ-3),5,3',4'-三羟基黄酮(Ⅳ-4),4',7-二甲氧基-5-羟基黄酮(Ⅳ-5),二十八碳烷(Ⅳ-6),三十二碳烷(Ⅳ-7),4S,7S-金色酰胺醇酯(Ⅳ-8),7-甲氧基-4',5-二羟基黄酮(Ⅳ-9),β-谷甾醇(Ⅳ-10)。
结论:从棉毛橐吾中分离鉴定的27个化合物中,2个为新化合物(Ⅰ-10和12),首次报道18个;从离舌橐吾中分离鉴定的10个化合物中,新化合物4个(Ⅱ-3,6a,8,9)及1个新天然产物(Ⅱ-7),除了Ⅱ-4外其它化合物都是首
次从该植物中得到;从两似蟹甲草中分离得到的4个化合物,都为首次从两似蟹甲草中得到;从芫花中分离得到10个化合物,其中2个化合物(Ⅳ-6,7)为首次报道,并利用二维核磁谱对化合物Ⅳ-8的~1HNMR和~(13)CNMR数据进行了全部归属,对离舌橐吾中部分化合物活性测试,结果发现化合物Ⅱ-3对肺癌细胞(A849)有较强的抑制作用(IC_(50)值1.93μg/mL)。
Objective:The first part,Ligularia vellerea(Ligularia genus of Compositae) was used to deal with hepatitis as a folk medicine.For studying the material foundation of Ligularia vellerea,someone had isolated and identified 20 compounds from it.It was reinvestigated for more knowledge.The second part,the plant Ligularia veitchiana grows aside the rivers,hillsides,woods in the elevation.The plant was disributed in the northemwest of Yunnan,Sichun,Guizhou,the west of Hubei,the southernwest of Gansu,the south of Shanxi.It was used to deal with the diseases such as influenza,cough,ulcer and pulmonary tuberculosis as a folk medicine.Up to now,Many eremophilane-type sesquiterpenes were isolated from the plants.We reinvestigated it from Funiu Mountains of Henan Province(the plant of the Mountains was not studied),to plan to isolate different active compounds.The third part,Cacalia ambigua belongs to Cacalia genus(Compositae).The Cacalia genus was distributed in easternnorth of Asia and America.50 species of the genus were grown in China,among them,26 species were used to cure tracheitis,arthritis and cough and so on.We systematically consult the Scifinder data and find no results about the chemical research of Cacalia ambigua.The two genus of Cacalia and Ligularia have kinsfolk relationship on plant taxalogy.Many plants of Cacalia genus have a number of eremophilane-type sesquiterpenoids.To search for the material foundation of this plant and enlarge the knowledge of plant chemotaxology,we studied the chemical constituents.The fourth part,the flowers of Daphne genkwa Sieb.et Zucc. (Thymelaeaceae) were used to detoxify and to release the unneccessary water of body as the common traditional chinese medicine.The quality standard of this medicine has not been worked out.So we studied on the chemical constituents that could be part of study on medicinal quality standard of yuanhua.
Methods:We used all kinds of modern chromatographic techniques including silica gel,Sephadex LH-20,MCI gel CHP-40P,Toyopearl HW-40(C) and RP-18 and so on to isolate the chemical constituents of the four plants,and then we identified the compounds and assigned the spectral data of them by modern spectroscopic techniques including ~1H NMR,~(13)C NMR,2D NMR and MS.Anticancer activity of several compounds from Ligularia veitchiana were screened by MTT.
Results:51 compounds were isolated from these four plants.Among them,27 compounds from Ligularia vellerea were isolated and determined:1, 5-dicaffeoylquinic acid(Ⅰ-1),3,5-dicaffeoylquinic acid(Ⅰ-2),eremophil-7(11)-en-12, 8α(14β,6α) -dioxide(Ⅰ-3),p- hydroxyacetophenone(Ⅰ-4),7-hydroxycoumarin(Ⅰ-5),3, 4- dihydroxyacetophenone(Ⅰ-6),4-methoxy-2',4',3-trihydroxy-chalone (butein-4-methyl ester)(Ⅰ-7),2',4',4-trihydroxy-chalone(Ⅰ-8),4'-methoxy-7, 3'-dihydroxyflavone(Ⅰ-9),4-[(3,4-dihydroxyl)-cinnamyl]-cinnamyl methyl ester(Ⅰ-10),p-hydroxycoumaric acid methyl ester(Ⅰ-11a),p-hydroxycoumaric acid(Ⅰ-11b),2,5-dihydroxyl-6,7-dimethyl-naphthoquinone(Ⅰ-12),4-O-caffeoylquinic acid(Ⅰ-13),3,4-dihydroxycoumaric acid(Ⅰ-14),6,7-dihydroxycoumarin(Ⅰ-15), 3-O-caffeoylquinic acid(Ⅰ-16),8β-methoxyeremophil-7(11)-en-12,8α(14β,6α) -dioxide(Ⅰ-17),furoeremophil-15β,6α-oxide(Ⅰ-18),tiglic acid(Ⅰ-19),β-sitosterol (Ⅰ-20),8β-hydroxyeremophil-7(11)-en-12,8α(14β,6α) -dioxide(Ⅰ-21),1-glycerol palmitic acid ester(Ⅰ-22),Ⅰ-23 Daucosterol(I-23),Palmitic acid(Ⅰ-24),1-25 Nonadecanoic acid methyl ester(Ⅰ-25),eremophil-7(11),8-dien-12,8α(14β,6α) -dioxide(Ⅰ-26).
10 compounds from Ligularia veitchiana were isolated and determined:1,3-dicaffeoylquinic acid(Ⅱ-1),1,5- dicaffeoylquinic acid(Ⅱ-2), eremophilan-6-en-11-ol(Ⅱ-3),β-sitosterol(Ⅱ-4),eremophil-6α-hydroxy-8α,12-olide (Ⅱ-5),10β-H-6β,8β-dihydroxyeremophil-8α,12 -oxide(Ⅱ-6),10α-H-6α, 8α-dihydroxyeremophil-8β,12 -oxide(Ⅱ-6a),1(10)-en-3-oxoeremophilan-11-ol(Ⅱ-7), (7α,9α,10α)-9,10-epoxy- eremophilan-11-ol(Ⅱ-8), 10α,11-endo-peroxide-1(2)-en-7α-hydroeremophilane(Ⅱ-9).
4 compounds from Cacalia ambigua were isolated,ingcluding 2 ent-kaurane-type diterpenes(ent-kaur-16-en-19-oic acid(Ⅲ-2) and 13,β-kanr-16β-ol (Ⅲ-3)),β-sitosterol(Ⅲ-1) and 1 glycerol ester(1-glycerol cerotic acid ester,Ⅲ-4 ),
10 compounds from Daphne genkawa were isolated:daphnoretin(Ⅳ-1),5,7,3', 4'-tetrahydroxyflavonone(Ⅳ-2),5,7,4'-trihydroxyflavonone(Ⅳ-3),5,3', 4'-hydroxyflavonone(Ⅳ-4),4',7-dimethoxy-5-hydroxyflavonone(Ⅳ-5), Octacosane(Ⅳ-6),Dotriacontane(Ⅳ-7),4S,7S-aurantiamide acetate(Ⅳ-8), 7-methoxy-4',5-dihydroxyflavonone(Ⅳ-9),β-sitosterol(Ⅳ-10).
Conclusions:Among 27 compounds from Ligularia vellerea,2 of them were new(Ⅰ-10 and 12),18 of them were isolated from the plant for the first time.Among 10 compounds from Ligularia veitchiana,4 of them were new(Ⅱ-3,6a,8,9) and one(Ⅱ-7) was natural product,others except forⅡ-4 were isolated from the plant for the first time.4 compounds from Cacalia ambigua were isolated for the first time. Among 10 compounds from Daphne genkawa,2 of them(Ⅳ-6 and 7) were isolated from the plant for the first time.~1H NMR and ~(13)C NMR data ofⅣ- 8 were assigned by 2D NMR.The results of the bioactivity test for the several compounds from Ligularia veitchiana showed thatⅡ-3 can restrain the lung cancer cells(A849)(IC_(50) value 1.93μg/mL).
引文
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