次声对骨细胞成骨效应及相关机制的实验研究
摘要
次声波是频率在0.0001～20Hz范围内的机械振动波。次声波在空气传播中具有传播远、衰减少,穿透力强等物理学特性,次声广泛存在自然界、生活环境、生产环境和军事环境中。
一定声压级水平的次声波作用一定时间可致人体功能障碍,甚至器质性损伤。因此,研究次声的发生、传播、生物学作用机制及制订相应的防护措施已越来越受到人们的重视。特别是近年来,一些发达国家投入大量的人力和财力,用于研究次声的生物学效应,并重视作为非致死性新概念武器——次声武器在战争中的作用研究,相关的文献报道也逐年增多。
同时,较低声压级水平和较短时间作用可能会具有良好的刺激效应,因此低强度的次声在医疗上的应用也引起了关注,例如研究应用次声治疗慢性胆囊炎、屈光不正等。骨组织作为对应力敏感的组织部分,次声波对骨生成重建的研究目前尚未见报告。
本课题观察了较低作用剂量的次声对骨生成的促进作用,并进一步采取体外培养的方式,研究观察较低强度的次声对成骨样细胞增殖分化的影响以及对细胞外基质OPN和ON的mRNA表达和细胞骨架F-actin表达的影响,探讨较低作用剂量的次声促进骨生成的机理。课题共分四部分实验:
第一部分次声改善雄性去势大鼠骨质疏松的试验研究
目的:观察较低强度的次声波作用于雄性去势大鼠后,其股骨骨质变化的情况。
方法:32只3月龄SD雄性大鼠,随机分为正常组、去势组、去势后4Hz次声作用组和去势后20Hz次声作用组,大鼠行双侧睾丸切除术(正常组行假手术)。次声压力舱系统及次声检测系统由第四军医大学与航天工业总公司及中科院声学所协作研制。各组大鼠术后分组置于次声压力舱内进行次声作用,其中前两组无次声输出;后两组次声参数分别为4Hz/100dB和20Hz/100dB;每组30min/d,每周作用5日,连续作用12周后处死。在处死大鼠前的第11d和第3d给与钙黄绿素(5mg/kg)和盐酸四环素(25mg/kg)皮下注射,进行活体荧光标记;麻醉处死后从心脏抽取血液2ml,放射免疫法测定血清骨钙素(BGP)浓度;用DPX-IQ型骨密度仪测定左股骨远端骨密度(BMD);取右侧股骨远端制作脱钙石蜡切片,测定骨小梁面积百分比(Tb·Ar)、骨小梁宽度(Tb·Th)、骨小梁间隙(Tb·Sp)以及骨小梁数目(Tb·N);取右股骨近端制备硬骨切片,荧光显微镜下观察钙黄绿素和四环素标记的双标线,计算骨膜矿化沉积率(MAR)。
结果:雄性去势大鼠的血清BGP较正常组降低(p<0.05),MAR减少(p<0.05),骨小梁宽度、条数、小梁百分比减少,小梁间隙增加(p<0.05);4Hz/100dB的次声作用组,显著增加了MAR和小梁宽度、条数等形态学指标(p<0.05),血清BGP也有增加趋势;20Hz作用组仅骨小梁宽度和骨小梁数目较去势组改善(p<0.05),余各项指标未有显著变化;各组股骨远端BMD无显著变化。
第二部分次声对成骨样细胞生物学特性的影响
目的:研究了100dB次声波对成骨样细胞MC3T3生物学特性的影响。
方法:成骨样细胞MC3T3按1×104/ml浓度接种在4个24孔培养板中,分为4组,置于次声压立舱中,分别以不同频率(4Hz、12Hz、20Hz)对细胞进行100dB次声作用,30min/d,对照组无次声输出。观察次声作用5日后细胞增殖、碱性磷酸酶(ALP)活性和骨钙素(OCN)的表达等方面的变化。
结果:发现次声作用组MC3T3细胞增殖速度增加,4Hz组和12Hz组细胞密度第3日起增加显著(p<0.05),20Hz组第5日细胞密度增加显著(p<0.05);各频率组和对照组的细胞ALP表达均较低,但无显著差异(p﹥0.05);各频率组细胞OCN表达较对照组明显增高(p<0.01)。
第三部分次声作用后MC3T3细胞骨桥素和骨粘连蛋白mRNA表达
目的:研究100dB不同频率次声对成骨细胞细胞外基质骨桥素(osteopontin,OPN)和骨粘连蛋白(osteonectin,ON)mRNA的影响。
方法:采用4个6孔培养板进行小鼠成骨样细胞系MC3T3细胞的培养,分别设为对照组、4Hz/100dB组、12Hz/100dB组、20Hz/100dB组。各组进行相应参数的次声波作用,对照组无次声输出,30min/d,在第3d次声作用后2h、8h分别进行骨桥素(OPN)及骨粘连蛋白(osteonectin,ON)mRNA原位杂交染色,光学显微镜下进行图像分析,测定细胞阳性物质平均灰度,进行分析。
结果:各组次声波作用后2h和4h的时间点,OPN mRNA、ON mRNA含量均较对照组显著升高(p<0.05),各次声作用组间差异不显著(p>0.05);8h后各次声作用组的mRNA含量下降,和对照组差别不显著(p>0.05)。
第四部分:次声作用后MC3T3细胞骨架蛋白的动态表达
目的:研究4Hz/100dB、12Hz/100dB、20Hz/100dB的次声作用后,小鼠成骨样细胞细胞MC3T3的细胞骨架F-actin表达的改变。
方法:将MC3T3细胞接种爬片同第三部分实验,分为对照组和4Hz/100dB、12Hz/100dB、20Hz/100dB的次声暴露组。各组分别接受次声作用30min/d,对照组无次声输出。第3d于暴露后2h、4h、8h不同时间,对细胞进行F-actin的免疫荧光染色,应用激光扫描共聚焦显微镜,测定单个细胞F-actin的平均荧光强度,观察细胞F-actin的表达改变。
结果对照组细胞大部分荧光物质呈弥漫状态,胞膜荧光较强,胞浆内少量肌动蛋白纤维丝,方向不规则,长短不一;不同频率次声作用后2h,各次声作用组均可看到胞浆中微丝F-actin明显粗大纤长,荧光物质大多为较长的粗大应力丝,沿细胞纵轴排列较多,数量及荧光强度明显增加(p<0.05);在不同频率次声作用后4h和8h,次声暴露组的细胞F-actin仍处于较高表达状态。不同频率次声作用组细胞的F-actin变化趋势较一致,各组在各时间点未见明显差异(p>0.05)。
综上所述,本课题结果提示:
1. 4Hz /100dB的次声30min/d共进行12周(每周5日)的作用,可以促进松质骨的生成,增加骨膜矿化沉降率,从而改善了雄性去势所造成的骨质疏松程度。
2.体外MC3T3成骨样细胞培养方式发现:100dB次声波30min作用可以促进成骨样细胞的体外增殖和分泌功能,4Hz对细胞体外增殖促进较明显,而20Hz对促进细胞成熟分化作用较明显。
3. 100dB的不同频率次声每日30min作用能促进成骨细胞的OPN及ON的mRNA表达一过性增高,次声作用8h后mRNA的表达减弱。
4. 100dB的不同频率次声作用30min/d,还可诱导F-actin表达的增强,这种增强改变在次声作用8h后仍未见减弱。
5.体外细胞培养实验在4Hz、12Hz、20Hz间未观察到明确的“频率窗”效应。
As the audible sound and ultrasound, infrasound is a mechanical vibration wave with frequency in range of 0.0001 to 20Hz. With the physical characteristics of less attenuation in long distance spreading and strong penetration, infrasound is harmful to human and leads to body dysfunction or a even severe damage when the intensity comes to a enough certain degree. So people are becoming increasingly aware of the importance in studying the mechanisms of the occurrence, spreading, biological effects and preventive measures of the infrasound.
Infrasound is existed widely in nature. It can be detected in our living condition, productive and military environments. In recent years many developed counties throw into a great amount of manpower and money in studying biological effects of infrasound, also they are gradually paying much attention to infrasound doing as a new concept weapon. Articles and reports about the infrasound are being increased year by year.
However, the low density infrasound maybe has good effects in medical just like other physiology agents. Some reports founded the low density infrasound can be used in treatment for ametropia, keratitis, anorexia. Being a mechanical wave, infrasound as an elastic spring forces produces a mechanical stress which something likes a pull and press alternately forces. As we kone, bone is very sensitive to strain, it can gain modeling when in the adapted strain windows, but no article about the low density infrasound on bone can be seen in present.
In the thesis, we observed the effects of bone modeling by low density infrasound on osteoporosis rats, and then to investigate the cellular proliferation , ALP activity , osteocalin (OCN) synthesis, extracelllular matrix(OPN/ ON ) mRNA level and cytoskeletal F actin expresion in vitro, to explore the infrasound signal transduction pathway into intracellular caused the bio-effects.
This thesis is composed of four parts as follow: Part one: Effect of infrasound for the prevention of osteoporosis in orchiectomized SD rats
Objective:To determine the preventive effects of lower density infrasound pressure on bone loss in orchiectomied rats. Method: divided 32 SD male rats into the control group, osteoporosis group, 4Hz infrasound group and 20Hz infrasound group. The rats in last 3 groups had been orchiectomied. Every group was expoused under infrasound of 4, and 20Hz individually 100dB output 30min each day while the control group and the osteoporosis group no infrasound output. After 12 weeks ( every group expoused 5d per week ), we observed the change of serum osteocalcin (OCN), bone mineral density (BMD), mineralization rate (MAR) and hisomorphometric measurement and calculations of distal femur with imaging analysis process.
Result: The osteoporosis group was obversly decresed in MAR and hisomorphometric measurement and calculations of distal femur than the control group (p<0.05 ). There was significantly increased in MAR and trabecular area, trabecular thickness, trabecular number in 4 Hz groups vs the osteoporosis group (p<0.05 ). In 4Hz group the serum osteocalcin also increased but no significant difference with the osteoporosis group. But in 20Hz group there were only increased in trabecular thickness, trabecular number ( p<0.05 ), the other measurement were no any differences with the osteoporosis group (p>0.05 ). There were no significant chang in BMD among the osteoprosis group and infrasound groups (p>0.05 ).
Part two: Effect on biological characteristics of Osteoblast-like cells exposed by infrasound in vitro
Objective:To determine the effects of infrasound pressure on biological characteristics of ostoblast-like cells,MC3T3-E1,in vitro.
Method: After primary culture, and devided into 4 groups, the osteoblast-like cells, MC3T3-E1,were exposed by infrasound with intensity output of 100dB and frequency of 4, 12 and 20Hz individually for 30min each day. The cellular proliferation was detected by cell counting method. Biochemical analysis and radio-immunological method were used to measure alkaline phosphatease activity (ALP) and osteocalin (OCN) synthesis after 5 days.
Result: The cell proliferation in experimental groups showed obviously faster than that in control group. The cell concentration in 4 and 12Hz groups was significantly increased from the 3rd day than in control group(p<0.05). Anyway, the same tendency could be observed in 20Hz group till the 5th day (p<0.05). There were not any differences between control group and experimental groups in ALP activity(p>0.05). The secretion of OCN was greatly increased in experimental groups(p<0.01).
Part three: Effects of infrasound on osteonectin and osteopontin mRNA level of osteoblast-like cells MC3T3 in vitro
Objective: To determine the effects of lower density infrasound pressure on osteopontin(OPN) and osteonectin(ON)mRNA level of osteoblast-like cells MC3T3.
Method: After primary culture, the cells were exposed by infrasound with intensity output of 100dB and frequency of 4, 12 and 20Hz individually for 30min each day. After the 3rd day, .
Result: Those infrasound exposured groups were significantly increased in the mRNA level of ON and OPN vs. the control group in 2h and 4h (p<0.05). There was no any differences between every groups and the control group in 8h in the mRNA level of OPN and ON (p>0.05).
Part four: The effect of low density infrasound on filament F- actin of osteoblast MC3T3
Objective:To study the alterations in expression of F-actin of osteoblast MC3T3 exposed by low density infrasound.
Methods: After the primary cultured on coverglass , and divided into four groups just like the experiment part three, the cells in experimental groups were exposed by infrasound with intensity output of 100dB and frequency of 4Hz, 12Hz and 20Hz individually for 30min/d. The cells in control group were managed in the same environment as experimental groups with intensity output of 0dB. Laser scanning confocal microscope was used to examine the changes of F-actin after immunofluorescent staining and the photos were taken for further analysis of the cells` average fluorescence by spectrofluorimetric quantification.
Results: In control group, most fluorescein-labelled substance was in diffusion states. Less actin filaments were tenuously short without direction. Correspondingly F-actin in the cells of experimental groups was thick long and showed in longitudinal arrangement after the exposure. The intensity of fluorescence was significantly increased. In every experiment group, there was a membrane-like boundary strong fluorescence. . More F-actin expression in experimental groups were being kept obviously increasing in 2h , 4h and 8h than the control group (p<0.05). However, there was not any significantly different in F-actin expression between the experimental groups ( p>0.05). Anyway cells in experimental groups showed a synchronous change.
Conclusion:
1. It could prevent bone loss in orchiectomied rats by expoused with 4 Hz/100dB infrasound 30min/d. There was significantly increased in MAR and trabecular area, trabecular thickness, trabecular number in 4Hz/100dB groups vs the osteoporosis group.
2. It could promote proliferation of the osterblast-like cells by exposured with 4Hz/100dB infrasound for 30min each day in vitro. and promote secretion of the cells by 20Hz/100dB infrasound for 30min/d.
3. The infrasound of 4Hz/100dB, 12Hz/100dB and 20Hz/100dB, 30min/d could promote the mRNA level of 0PN and 0N. This effects at last for 4 hours. There were no obviously different among 4Hz, 12Hz and 20Hz.
4. F-actin expression can be increased in osteoblast MC3T3 exposed 30min/d with infrasound whioch intensity output of 100dB and frequency of 4Hz, 12Hz, 20Hz . The expression increasing of F-actin still can be founded in 8h after exposed under the low density infrasound.
5. No special frequency effects can be founded in those cells-cultured expriments.
一定声压级水平的次声波作用一定时间可致人体功能障碍,甚至器质性损伤。因此,研究次声的发生、传播、生物学作用机制及制订相应的防护措施已越来越受到人们的重视。特别是近年来,一些发达国家投入大量的人力和财力,用于研究次声的生物学效应,并重视作为非致死性新概念武器——次声武器在战争中的作用研究,相关的文献报道也逐年增多。
同时,较低声压级水平和较短时间作用可能会具有良好的刺激效应,因此低强度的次声在医疗上的应用也引起了关注,例如研究应用次声治疗慢性胆囊炎、屈光不正等。骨组织作为对应力敏感的组织部分,次声波对骨生成重建的研究目前尚未见报告。
本课题观察了较低作用剂量的次声对骨生成的促进作用,并进一步采取体外培养的方式,研究观察较低强度的次声对成骨样细胞增殖分化的影响以及对细胞外基质OPN和ON的mRNA表达和细胞骨架F-actin表达的影响,探讨较低作用剂量的次声促进骨生成的机理。课题共分四部分实验:
第一部分次声改善雄性去势大鼠骨质疏松的试验研究
目的:观察较低强度的次声波作用于雄性去势大鼠后,其股骨骨质变化的情况。
方法:32只3月龄SD雄性大鼠,随机分为正常组、去势组、去势后4Hz次声作用组和去势后20Hz次声作用组,大鼠行双侧睾丸切除术(正常组行假手术)。次声压力舱系统及次声检测系统由第四军医大学与航天工业总公司及中科院声学所协作研制。各组大鼠术后分组置于次声压力舱内进行次声作用,其中前两组无次声输出;后两组次声参数分别为4Hz/100dB和20Hz/100dB;每组30min/d,每周作用5日,连续作用12周后处死。在处死大鼠前的第11d和第3d给与钙黄绿素(5mg/kg)和盐酸四环素(25mg/kg)皮下注射,进行活体荧光标记;麻醉处死后从心脏抽取血液2ml,放射免疫法测定血清骨钙素(BGP)浓度;用DPX-IQ型骨密度仪测定左股骨远端骨密度(BMD);取右侧股骨远端制作脱钙石蜡切片,测定骨小梁面积百分比(Tb·Ar)、骨小梁宽度(Tb·Th)、骨小梁间隙(Tb·Sp)以及骨小梁数目(Tb·N);取右股骨近端制备硬骨切片,荧光显微镜下观察钙黄绿素和四环素标记的双标线,计算骨膜矿化沉积率(MAR)。
结果:雄性去势大鼠的血清BGP较正常组降低(p<0.05),MAR减少(p<0.05),骨小梁宽度、条数、小梁百分比减少,小梁间隙增加(p<0.05);4Hz/100dB的次声作用组,显著增加了MAR和小梁宽度、条数等形态学指标(p<0.05),血清BGP也有增加趋势;20Hz作用组仅骨小梁宽度和骨小梁数目较去势组改善(p<0.05),余各项指标未有显著变化;各组股骨远端BMD无显著变化。
第二部分次声对成骨样细胞生物学特性的影响
目的:研究了100dB次声波对成骨样细胞MC3T3生物学特性的影响。
方法:成骨样细胞MC3T3按1×104/ml浓度接种在4个24孔培养板中,分为4组,置于次声压立舱中,分别以不同频率(4Hz、12Hz、20Hz)对细胞进行100dB次声作用,30min/d,对照组无次声输出。观察次声作用5日后细胞增殖、碱性磷酸酶(ALP)活性和骨钙素(OCN)的表达等方面的变化。
结果:发现次声作用组MC3T3细胞增殖速度增加,4Hz组和12Hz组细胞密度第3日起增加显著(p<0.05),20Hz组第5日细胞密度增加显著(p<0.05);各频率组和对照组的细胞ALP表达均较低,但无显著差异(p﹥0.05);各频率组细胞OCN表达较对照组明显增高(p<0.01)。
第三部分次声作用后MC3T3细胞骨桥素和骨粘连蛋白mRNA表达
目的:研究100dB不同频率次声对成骨细胞细胞外基质骨桥素(osteopontin,OPN)和骨粘连蛋白(osteonectin,ON)mRNA的影响。
方法:采用4个6孔培养板进行小鼠成骨样细胞系MC3T3细胞的培养,分别设为对照组、4Hz/100dB组、12Hz/100dB组、20Hz/100dB组。各组进行相应参数的次声波作用,对照组无次声输出,30min/d,在第3d次声作用后2h、8h分别进行骨桥素(OPN)及骨粘连蛋白(osteonectin,ON)mRNA原位杂交染色,光学显微镜下进行图像分析,测定细胞阳性物质平均灰度,进行分析。
结果:各组次声波作用后2h和4h的时间点,OPN mRNA、ON mRNA含量均较对照组显著升高(p<0.05),各次声作用组间差异不显著(p>0.05);8h后各次声作用组的mRNA含量下降,和对照组差别不显著(p>0.05)。
第四部分:次声作用后MC3T3细胞骨架蛋白的动态表达
目的:研究4Hz/100dB、12Hz/100dB、20Hz/100dB的次声作用后,小鼠成骨样细胞细胞MC3T3的细胞骨架F-actin表达的改变。
方法:将MC3T3细胞接种爬片同第三部分实验,分为对照组和4Hz/100dB、12Hz/100dB、20Hz/100dB的次声暴露组。各组分别接受次声作用30min/d,对照组无次声输出。第3d于暴露后2h、4h、8h不同时间,对细胞进行F-actin的免疫荧光染色,应用激光扫描共聚焦显微镜,测定单个细胞F-actin的平均荧光强度,观察细胞F-actin的表达改变。
结果对照组细胞大部分荧光物质呈弥漫状态,胞膜荧光较强,胞浆内少量肌动蛋白纤维丝,方向不规则,长短不一;不同频率次声作用后2h,各次声作用组均可看到胞浆中微丝F-actin明显粗大纤长,荧光物质大多为较长的粗大应力丝,沿细胞纵轴排列较多,数量及荧光强度明显增加(p<0.05);在不同频率次声作用后4h和8h,次声暴露组的细胞F-actin仍处于较高表达状态。不同频率次声作用组细胞的F-actin变化趋势较一致,各组在各时间点未见明显差异(p>0.05)。
综上所述,本课题结果提示:
1. 4Hz /100dB的次声30min/d共进行12周(每周5日)的作用,可以促进松质骨的生成,增加骨膜矿化沉降率,从而改善了雄性去势所造成的骨质疏松程度。
2.体外MC3T3成骨样细胞培养方式发现:100dB次声波30min作用可以促进成骨样细胞的体外增殖和分泌功能,4Hz对细胞体外增殖促进较明显,而20Hz对促进细胞成熟分化作用较明显。
3. 100dB的不同频率次声每日30min作用能促进成骨细胞的OPN及ON的mRNA表达一过性增高,次声作用8h后mRNA的表达减弱。
4. 100dB的不同频率次声作用30min/d,还可诱导F-actin表达的增强,这种增强改变在次声作用8h后仍未见减弱。
5.体外细胞培养实验在4Hz、12Hz、20Hz间未观察到明确的“频率窗”效应。
As the audible sound and ultrasound, infrasound is a mechanical vibration wave with frequency in range of 0.0001 to 20Hz. With the physical characteristics of less attenuation in long distance spreading and strong penetration, infrasound is harmful to human and leads to body dysfunction or a even severe damage when the intensity comes to a enough certain degree. So people are becoming increasingly aware of the importance in studying the mechanisms of the occurrence, spreading, biological effects and preventive measures of the infrasound.
Infrasound is existed widely in nature. It can be detected in our living condition, productive and military environments. In recent years many developed counties throw into a great amount of manpower and money in studying biological effects of infrasound, also they are gradually paying much attention to infrasound doing as a new concept weapon. Articles and reports about the infrasound are being increased year by year.
However, the low density infrasound maybe has good effects in medical just like other physiology agents. Some reports founded the low density infrasound can be used in treatment for ametropia, keratitis, anorexia. Being a mechanical wave, infrasound as an elastic spring forces produces a mechanical stress which something likes a pull and press alternately forces. As we kone, bone is very sensitive to strain, it can gain modeling when in the adapted strain windows, but no article about the low density infrasound on bone can be seen in present.
In the thesis, we observed the effects of bone modeling by low density infrasound on osteoporosis rats, and then to investigate the cellular proliferation , ALP activity , osteocalin (OCN) synthesis, extracelllular matrix(OPN/ ON ) mRNA level and cytoskeletal F actin expresion in vitro, to explore the infrasound signal transduction pathway into intracellular caused the bio-effects.
This thesis is composed of four parts as follow: Part one: Effect of infrasound for the prevention of osteoporosis in orchiectomized SD rats
Objective:To determine the preventive effects of lower density infrasound pressure on bone loss in orchiectomied rats. Method: divided 32 SD male rats into the control group, osteoporosis group, 4Hz infrasound group and 20Hz infrasound group. The rats in last 3 groups had been orchiectomied. Every group was expoused under infrasound of 4, and 20Hz individually 100dB output 30min each day while the control group and the osteoporosis group no infrasound output. After 12 weeks ( every group expoused 5d per week ), we observed the change of serum osteocalcin (OCN), bone mineral density (BMD), mineralization rate (MAR) and hisomorphometric measurement and calculations of distal femur with imaging analysis process.
Result: The osteoporosis group was obversly decresed in MAR and hisomorphometric measurement and calculations of distal femur than the control group (p<0.05 ). There was significantly increased in MAR and trabecular area, trabecular thickness, trabecular number in 4 Hz groups vs the osteoporosis group (p<0.05 ). In 4Hz group the serum osteocalcin also increased but no significant difference with the osteoporosis group. But in 20Hz group there were only increased in trabecular thickness, trabecular number ( p<0.05 ), the other measurement were no any differences with the osteoporosis group (p>0.05 ). There were no significant chang in BMD among the osteoprosis group and infrasound groups (p>0.05 ).
Part two: Effect on biological characteristics of Osteoblast-like cells exposed by infrasound in vitro
Objective:To determine the effects of infrasound pressure on biological characteristics of ostoblast-like cells,MC3T3-E1,in vitro.
Method: After primary culture, and devided into 4 groups, the osteoblast-like cells, MC3T3-E1,were exposed by infrasound with intensity output of 100dB and frequency of 4, 12 and 20Hz individually for 30min each day. The cellular proliferation was detected by cell counting method. Biochemical analysis and radio-immunological method were used to measure alkaline phosphatease activity (ALP) and osteocalin (OCN) synthesis after 5 days.
Result: The cell proliferation in experimental groups showed obviously faster than that in control group. The cell concentration in 4 and 12Hz groups was significantly increased from the 3rd day than in control group(p<0.05). Anyway, the same tendency could be observed in 20Hz group till the 5th day (p<0.05). There were not any differences between control group and experimental groups in ALP activity(p>0.05). The secretion of OCN was greatly increased in experimental groups(p<0.01).
Part three: Effects of infrasound on osteonectin and osteopontin mRNA level of osteoblast-like cells MC3T3 in vitro
Objective: To determine the effects of lower density infrasound pressure on osteopontin(OPN) and osteonectin(ON)mRNA level of osteoblast-like cells MC3T3.
Method: After primary culture, the cells were exposed by infrasound with intensity output of 100dB and frequency of 4, 12 and 20Hz individually for 30min each day. After the 3rd day, .
Result: Those infrasound exposured groups were significantly increased in the mRNA level of ON and OPN vs. the control group in 2h and 4h (p<0.05). There was no any differences between every groups and the control group in 8h in the mRNA level of OPN and ON (p>0.05).
Part four: The effect of low density infrasound on filament F- actin of osteoblast MC3T3
Objective:To study the alterations in expression of F-actin of osteoblast MC3T3 exposed by low density infrasound.
Methods: After the primary cultured on coverglass , and divided into four groups just like the experiment part three, the cells in experimental groups were exposed by infrasound with intensity output of 100dB and frequency of 4Hz, 12Hz and 20Hz individually for 30min/d. The cells in control group were managed in the same environment as experimental groups with intensity output of 0dB. Laser scanning confocal microscope was used to examine the changes of F-actin after immunofluorescent staining and the photos were taken for further analysis of the cells` average fluorescence by spectrofluorimetric quantification.
Results: In control group, most fluorescein-labelled substance was in diffusion states. Less actin filaments were tenuously short without direction. Correspondingly F-actin in the cells of experimental groups was thick long and showed in longitudinal arrangement after the exposure. The intensity of fluorescence was significantly increased. In every experiment group, there was a membrane-like boundary strong fluorescence. . More F-actin expression in experimental groups were being kept obviously increasing in 2h , 4h and 8h than the control group (p<0.05). However, there was not any significantly different in F-actin expression between the experimental groups ( p>0.05). Anyway cells in experimental groups showed a synchronous change.
Conclusion:
1. It could prevent bone loss in orchiectomied rats by expoused with 4 Hz/100dB infrasound 30min/d. There was significantly increased in MAR and trabecular area, trabecular thickness, trabecular number in 4Hz/100dB groups vs the osteoporosis group.
2. It could promote proliferation of the osterblast-like cells by exposured with 4Hz/100dB infrasound for 30min each day in vitro. and promote secretion of the cells by 20Hz/100dB infrasound for 30min/d.
3. The infrasound of 4Hz/100dB, 12Hz/100dB and 20Hz/100dB, 30min/d could promote the mRNA level of 0PN and 0N. This effects at last for 4 hours. There were no obviously different among 4Hz, 12Hz and 20Hz.
4. F-actin expression can be increased in osteoblast MC3T3 exposed 30min/d with infrasound whioch intensity output of 100dB and frequency of 4Hz, 12Hz, 20Hz . The expression increasing of F-actin still can be founded in 8h after exposed under the low density infrasound.
5. No special frequency effects can be founded in those cells-cultured expriments.
引文
[1] 陈景藻.次声的产生及生物学效应. 见:总后勤部卫生部主编.医药卫生科学技术进展.北京:军事医学科学出版社,1997.194-197
[2] arstang M Long-distance. low-frequency elephant communication. J Comp Physiol A Neuroethol Sens Neural Behav Physiol, 2004, Oct; 190(10):791-805
[3] 施志远.动物预报地震的奥秘.北京:科学普及出版社,1993.50-51
[4] 李爱玲,闫夷升.次声波及其应用.现代物理知识. 2001.33-34
[5] 苏昉,苏骁.强地震临震预报的——前兆次声测量研究.石油大学学报(自然科学版),2000,24(6):106-114
[6] 谢金来,谢照华.1993 年 7 月 2 日日本北海道地震次声波.声学学报,1996,21(1)55-61
[7] 牟翔.环境次声测量.第四军医大学博士学位论文.2001.28-34
[8] Sand O, Karlsen HE. Detection of infrasound by the Atlantic cod. J Exp Biol, 1986,125:197-204
[9] 王在忠,张作明,郭群.次声的生物作用的研究进展.预防医学情报杂志,2001,17(5):342-344
[10] Karing W, Ludwik L. Burning oil fields as a source of infrasonic waves. J Low Freq Noise Vib, 1991,10(4):123-9
[11] Ерохин ВН.,и др.Реакция рецеп- торных кле- ток спирального органа на действие инф-равука.Шум,вибрац- ияи борьба с ними напроизводстве: Teз.докл.респ.конф.Л.:1979;92-93
[12] Карпова НИ. и T.д. Низкочастот- ные акустические колебания.М:Мед- ицина,1981,189-190
[13] Schulze J, Auer M, Werzi R. Low level radioactivity measurement in support of the CTBTO. Comprehensive Nuclear-Test –Ban Treaty Organization. Appl Radiat Isot, 2000,53(1-2):23-30
[14] 符文琛.工业次声的劳动卫生问题.职业医学,1985,12(2):47-49
[15] Hennrik M. Annoyance of audilbe infrasound. J Low Freq Noise Vib, 1987,6(1):1-17
[16] Backteman O,Kohler J, Sjoberg L. Infrasound-tutorial and review:part4. Low Freq Noise Vib, 1984,3(1):28-36
[17] 陈景藻.次声的产生及生物学效应.国外医学物理医学与康复分册,1999,19:15-17
[18] 范建中.超声波的物理学基础.见:陈景藻主编.现代物理治疗学.北京:人民军医出版社,2001,326
[19] Broner N. The effects of low frequence noise on people. Areie. Sound and Vibration, 1978,58(4):483-488
[20] Rice CG. Annoyance due to low frequence hums. British Medical Journal, 1994,30895:355.
[21] 苏昉,田维.2001 年 2-4 月强地震的前兆次声波测量研究. 地球物理学进展,2001,16(4):61-70
[22] Georges T M. HF Doppler studies of traveling ionospheric disturbances. J Atmos Terr Phys. 1968,30:735741
[23] 杜功焕,朱哲民,龚秀分.声学基础(上册)上海:上海科学技术出版社,1981,136-138
[24] Gavreau V. infrasound: Generateurs, Detecteurs. Proprietes physiques, effects biologigues. Acustica, 1966,17:1-3
[25] 屠焰,赵树卿.奇异的次声.北京:科学出版社,1979:3-4
[26] Batanov GV. Characteristics of etology of immediate hypersensitivity in condition of exposure to infrasound. Radiat Biol Radioecol, 1995,35(1):78-82
[27] 王冰水,陈景藻,郭国祯,等. 次声作用对 CHO 细胞膜影响的原子力显微镜观察,中华物理医学与康复杂志,2005,27(3):156-158
[28] 丘萍,温宁,姜勇,陈景藻.次声的物理存在及研究意义.中华预防医学杂志,2003,37(10):56-58
[29] 杜宝东,刘淑芳. 次声对人体及动物影响的研究进展.国外医学耳鼻咽喉科学分册,2001,25(2):99-102
[30] 王斌,陈景藻,易南.不同声强 8Hz 次声对小鼠学习能力的影响.第四军医大学学报,1997,18(5):442-445
[31] 魏智钧,李玲,陈景藻. 次声作用对大鼠记忆功能及隔内侧核和斜角带核胆碱能神经元表达的影响.中华物理医学与康复杂志,2001;23(2):83-85
[32] Janhunen, H.K. Combined Effects of Occupational Exposure. Helsinki, finland. Infrasound, 1984,134-139
[33] 费舟,章翔,王小峰.次声作用后鼠脑超微结构与血脑屏障改变,中华物理医学与康复杂志,1999,21(3):134-137
[34] 费舟,章翔关,李树合.次声对脑组织血栓素 A2、前列环素代谢的影响.中华物理医学与康复杂志,2000,22(5):270-272
[35] 刘卫,陈景藻,李玲,刘静,裘明途. 次声对兔脑电活动的影响. 中华物理医学与康复杂志,2002,24(5):269-271
[36] 叶琳,龚书明,黄晓峰,王枫,刘静,陈景藻.次声作用对大鼠大脑皮层超微结构的影响.第四军医大学学报,2002,23(9):856-858
[37] Takigawa. H. Effects of infrasound on vestibular function. J Low Freq Noise and Vib, 1991,151(3):4553
[38] Fu Guo-you, Chen Jing-zao, Jia Ke-Yong. Changes of Glutamate in brain of rats exposed to infrasound. J. Fourth Mil. Med. Univ, 1999. 20(4): 288-290
[39] Liu,Z.H., Chen,J.Z., Tang,Y., et al., Effects of infrasound on changes of intercellular Calcium ion concentration and on expression of RyRs in hippocampus of rat brain. J Low Freq Noise Vib,2005,23(3): 159～165.
[40] 赵乃坤.次声对动物生物学效应的研究.中华劳动卫生职业病杂志,1993,11(6):338
[41] 邢晓辉,王凌,陈景藻,饶志仁. 次声刺激诱导大鼠延髓内儿茶酚胺能神经元 FOS 的表达解放军医学杂志,1998,23(3):189-191
[42] Landstrom U. Laboratory and field studies on infrasound and its effects on human. J Low Frequeqency Noise and Vibration, 1987,6:29-34
[43] Izmerov Nf, Suvorov GA, Kuralesin NA, et al. Infrasound: bodys effects and hygienic regulation. Vestn Ross Akad Med Nauk, 1997,(7):39-46
[44] Kuralesin NA. Hygienic and biomedical aspects of the effects of infrasound. Med Tr Prom Ekol, 1997,(5):8-14
[45] 袁华,陈景藻,李玲. 8Hz 次声作用后大鼠脑热休克蛋白 70 的表达. 中华物理医学与康复杂志,1999,21(2):94-97
[46] 黄文晋,贾克勇,张萍,肖华胜,韩良辅,陈镔复,陈景藻,鞠躬.次声作用后大鼠下丘脑 HSP70 的表达.第四军医大学学报,2000,21(2):162-163
[47] 牟翔,陈景藻,李玲,邱建勇,贾克勇. 次声作用对小鼠脑中胶质纤维酸性蛋白表达和分布的影响.中华物理医学与康复杂志,2001,23(2):76-79
[48] 袁华,龙华,李玲,牟翔,邱建勇,刘静,陈景藻.次声作用后大鼠延髓星形胶质细胞和神经元反应的关系.中国康复理论与实践,2002,8(11):661-663
[49] 裴朝辉.次声对心血管的影响及机制的实验研究,第四军医大学博士学位论文,2004:69-80
[50] Lehoux S, Tedgui A. Shear and signal transduction in the endothelial cell Med Sci. 2004 ,20(5):551-6
[51] McCue S, Noria S, Langille BL. Shear-induced reorganization of endothelial cell cytoskeleton and adhesion complexes. Trends Cardiovasc Med. 2004,14(4):143-51
[52] 裴兆辉,陈景藻,朱妙章.次声诱导的大鼠心肌细胞凋亡和管内皮损伤,第四军医大学学报,2004,25(2):97-99
[53] 陈景藻.次声的存在及其基本生物效应和研究意义.中华物理医学与康复杂志, 1999, 21(3):131-133
[54] Нехорошев АС. Морфофункцио- нальные изменения в миокарде при действии инфраэвука Гиг и Сан 1991; 12: 56
[55] Kanelo S, Okumura K, Numaguchi Y. Melatonin scavenges hydroxyl radical and protects isolated rat hearts from ischemic reperfusion injury. Life Sci, 2000, 67:101-102
[56] 裴兆辉,朱妙章,陈景藻.8Hz,130dB 次声引起心肌组织超微结构的变化. 第四军大学学报,2004;25(11):991-993
[57] Nekhoroshev AS, Glinchikov VV. Morphofunctional changes in the myocardium under exposure to infrasound. NVB. Noise Vib. Bull, 1998,(12):56-58
[58] Gordelaze AS, Glinchilov VV, Usenko VR. Experimental myocardial ischemia caused by infrasound. Gig. Tr.Prof. Zabol, 1986,(6):30-33
[59] 田时秀,李金锡,胡冰.次声对人血压和脉搏的影响. 声学学报,1996,21(3):254-258
[60] 汤家骥.次声的损伤效应,解放军医学情报,1995,9(5):262-264
[61] Kuralesin NA. Hygienic and biomedical aspects of the effects of infrasound. Med Tr Prom Ekol, 1997,(5):8-14
[62] 冯勃,姜泗长,杨伟炎等.强次声波对豚鼠前庭终器超微结构造成的损伤. 中华航空航天医学, 2001,12(2):99-102
[63] Salt A.N., Demott. J.E. Longitudinal endolymph movements and endocochlear potential changes induced by stimulation of infrasound frequencies. J.acoust. Soc. AM, 1999,106(2):847-856
[64] Нехорошев АС.Механизм эффекта при действии инфразвука.Косм-Биол-Авиаком Мед,1990;24(6):39-41
[65] Нехорошев АС.Изучение механи- зма дейст-вия низкочастотных аус- тических колеба нии.Медицина труда и промышленная экология,1998;(5):26-29
[66] Yoshida A, R.Nagano. The influence of infrasound vibration upon cochlea. Sangyo Ika Daigaku Zasshi, 1986,8(suppl.):123-128
[67] 张术,黄维国,邱建华等.次声对豚鼠听阈的影响. 陕西医学杂志, 1998,27(4):253-254
[68] 刑晓辉,陈景藻. 次声刺激诱导大鼠听中枢 FOS 蛋白的表达. 中华物理医学杂志,1998;20(3):165-167
[69] Carricondo F, Bartolome MV, Vicente-Torres MA, et al. Sensitivity to glutamate neurotoxicity in different developmental periods of the rat cochlea. Adv Otorhinolaryngol 2002;59:91-5
[70] Fei zhou, Zhang Xiang, Lu Peiling et al. The changes and significance of cerebral ultrastructure and the expression of heat shock protein 70 in cortex after infrasonic damage. J Low Freq Noise Vib 2000;19(2):93-99
[71] 刘恩渝,章翔,费舟等. 次声作用下大鼠脑皮质内谷氨酸及其代谢型受体 I 亚型 mRNA 的变化. 第四军医大学学报, 2001;22(23):2137-2140
[72] 邢晓辉,贾克勇,陈景藻. 次声刺激后大鼠下丘 n-cNOS 阳性神经元 FOS 蛋白表达,中华劳动卫生职业病杂志,1999,17(4):214-216
[73] 韩良辅,肖华胜陈景藻。次声作用引起大鼠下丘脑 c-fos mRNA 与CRFmRNA 的表达,第四军医大学学报。1999;20(1):1-3
[74] 王冰水. 博士学位论文,第四军医大学. 西安,2005,60-72
[75] 王在忠,张作明,王冰,郭群,李莉. 次声作用后大鼠视网膜组织中 GFAP 与 VEGF 的表达.眼外伤职业病杂志,2002,24(1):6-8
[76] 曹燕,邱萍,惠延年. 次声作用对大鼠血视网膜屏障超微结构的损伤. 2003,19(1):48
[77] 邱萍,张作明,姜勇,郭群,王冰,苟琳,陈景藻.次声暴露对大鼠血视网膜屏障超微结构的损害.中华眼科杂志 2002,38(8):499-501
[78] 王志平,王永建,王荣芬. 未来的超常规武器. 北京,解放军出版社,1985,64
[79] Sidorenko EI, Obrubov SA, Dreval AA, et al. Experiental study of morphologic changes in ocular tissue structures after exposure to infrasound pneumomassage. Vestn Oftalmol, 1996,112:17-19.
[80] 苟琳,张作明,郭守一,贾克勇,邱萍,李莉,郭群,陈景藻. 次声作用对大鼠视网膜 Muller 细胞及血管内皮细胞增殖力的影响,中华航空航天医学杂志,2002,13(1):44-46
[81] 王在忠、张作明,王冰,郭群。次声对大鼠视觉电生理和视网膜超微结构的影响. 眼外伤职业病杂志,2001,23(6):614-616
[82] 石力. 硕士学位论文,第四军医大学,西安,2003,5
[83] 韩凤华,武毅军,陈景藻,黄晓峰,刘小玲,王喜青. 次声下大鼠肝细胞损伤作用的超微结构变化. 西南国防医药, 2003,13(5):465-467
[84] Нехорошев АС. Реакция гема- тоцитов на воздействие инфразв- ука, Гиг и Сан, 1991; 2 :45-47
[85] Lekseev, S.S., V.V. Glinchikov, V.R. Usenko. Reaction of liver cells to infrasound. Gig. Tr. Prof. Zabol, 1986,(9):131-132
[86].Nekhorohev As, Glinchikov VV, Reaction of hepatocytes toinfrasound exposure. Gig Sanit, 1991Feb:2:45-47
[87] Nekhoroshex AS, Glinchikov VV. Morphological research on the liver structures of experimental animals under the action of infrasound. Aviakosm Elolog Med. 1992,26(3):56-9
[88] Ekhoroshev As, Glinchikov VV. Morphological research on the liver structures of experimental animals under the action of infrasound Aviakosm Ekolog Med 1992,May-jun; 26(3):56-59
[89] .Branco N, Santos J, Monteiro E, Silva A, Ferreira J, Pereira M. The lung parenchyma in low frequency noise exposed Wistar rats. Rev Port Pneumol. 2004 Jan-Feb;10(1):77-85
[90] 李唯,贾克勇,李焕章,倪殿涛,陈景藻. 次声对大鼠肺组织损伤及肺氧合功能的影响. 中华劳动卫生职业病杂志.2001,19(3):194-197
[91] 李唯,白汝芬,倪殿涛,李焕章,陈景藻. 8Hz 和 16Hz 单次次声作用后大鼠肺组织匀浆中细胞因子 TNF-α及 IL-8 的变化. 中国急救医学,2002,22(3):127-129
[92] 崔芳,任雨笙,李玲,张荣庆,袁华,贾克勇,陈景藻. 不同声强8Hz 次声作用对大鼠 GSH-PX,SOD 活性及 MDA 含量的影响. 第四军医大学学报, 1999,20(9):743-4
[93] 魏亚宁,刘静,舒青等.次声暴露对小鼠睾丸细胞超微结构的影响,中华男科学,2002,8(5):323-325
[94] 魏亚宁,刘静,舒青等.次声暴露对小鼠卵巢细胞超微结构的影响,第四军医大学学报,2003,24(4):293-295
[95] 刘秀敏,甄荣,陈惠芳等. 次声对小鼠生育能力与免疫功能的影响,中华物理医学与康复杂志,2000,22(2):100-102
[96] Naoko N, Masanobu M, Yasukiyo Y, et al. Process and emergenceon the effects of infrasonic and low frequency noise on inhabitants. J Low Freq Noise Vib, 1989,8:87-99
[97] Nishimura K, Kuroda M, Yoshida Y et al. The pituitary adrenocortical response in rats and human subjects exposed to infrasound. J Low Freq Noise Vib, 1987,(6):18
[98] 赵志刚,陈景藻,张李燕. 次声作用后大鼠血浆中血管紧张素 II含量的改变. 中华物理医学与康复杂志,2001,23(2):83-85
[99] 赵乃坤. 次声对动物生物学效应的研究. 中华劳动卫生职业病杂志, 1993;11(6):338
[100] Колмаков ВН,Свидовый ВИ,Шлейкин АГ. Влияние низкочастотных ак-устических колебаний на некото- рые компоненты мембраны э р и т р о - ц и т о в . Г и г . Т р у да.1984;(10):48-49
[101] 张建保,贾克勇,李玲,五南林,杨继庆,巨宏博,陈景藻. 次声对大鼠血液流变特性的影响,第四军医大学学报,2001;22(5):465-7
[102] Wolff W,White A A,Panjabi M M,et al. Comparision of cyclic loading versus contant compression in the fracture in habbits . Bone and Jiont Surg(Am),1981,63:805～810
[103] Svidovyi VI, Kitaeva LV. Evaluation of cytogenetic activity in bone marrow cells exposed to infrasound (experimental data), Med Tr Prom Ekol, 1998,5:42-4
[104] 范云,杨春智. 次声(物理学教材),第四军医大学, 1995,3
[105] Osamn Okai. Effects of infrasound on respiratory functionof man. J Low Freq Noise and Vib, 1986;5(3):94-99
[106] Svidovyi VI , Kolmakov VN, Kuleba VA, Timofeeva VM. Change in the permeability and total ATPase activity of erythrocytes and blood plasma superoxide dismutase activity on exposure to infrasound in vitr.o. Gig Sanit. 1987May;(5):78-79
[107] Martini K, Opltova L. Human nonspecific response to sound stimulation. J Hyg Epidemiol Mivrobiol Immunol, 1986;30(2):139-44
[108] Idovyi VI, Kuklina OI. State of the hemolymph circulatory bed of conjunctiva as affected by infrasound. Gig Tr Prof Zabol, 1985;Jun(6):51-52
[109] 范建中.超声波的物理学基础.见:陈景藻主编,现代物理治疗学. 北京: 人民军医出版社,2001,328
[110] Balunov VD, Barsukov AF, Artamonova VG. Clinical and functional evaluation of health status of workers exposed to infrasound, noise and general vibration. Med Tr Prom Ekol. 1998;(5):22-26
[111] 黄其柏. 家用电器次声及其声辐射特征的研究. 噪声与振动控制,1997,17(5):6-8
[112] Pawlaczyk LM. Evaluation of occupational exposure to infrasonic noise in Poland. Int J Occup Med Environ Health. 1999;12(2):159-76
[113] Pawlaczyk LM, Augustynska D, Kczmarska KA, Sliwinska KM, Kamedula M. Revised maximum admissible intensity (MAI) values for infrasonic noise in work environment. Med Pr.2001;52(2):119-23
[114] Takahashi Y, Yonekawa Y, Kanada K, Maeda S. An infrasound experiment system for industrial hygiene. Ind Health. 1997 Oct;35(4):480-8
[115] 梁振福,黄锋,唐志文等. 护耳器对次声防护效果的观察. 航天医学与医学工程,2003,16(4):110-114
[116] 汤家骥. 次声武器杀伤因素的生物效应及防护. 人民军医,1995,426(5):5-6
[117] 王斌,陈景藻,易南. 次声对小鼠学习记忆能力的影响及防护. 航空军医,1997,25(3):142-143
[118] 刘思渝,费舟,章翔,陈景藻,刘先珍. 次声作用下大鼠脑皮质I、II 组代谢性谷氨酸受体 mRNA 表达及意义,中华物理医学与康复杂志,2001,23(5):284-287
[119] 李志刚,费舟,章翔,贾克勇,陈景藻,贺晓生,刘先珍. 次声作用后鼠脑CA1区mGluR1α表达改变及其拮抗剂MCPG的作用研究。解放军医学杂志,200126(2):104-106
[120] 张元菊,陈景藻,杨俊峰,王斌,刘静. 16Hz、130dB 次声对小鼠记忆功能和脑超微结构的影响及姜黄素的治疗作用研究,中华物理医学与康复杂志,2005,27(3):152-155
[121] Nekhoshev As, Glinchilov VV. Mechanism of the effect of infrasound on labybrinthine recetors. Kosm Biol Ariakosm Med, 1990,24(6):39-42
[122] 焦明若,张国民. 地震前兆复杂性成因机理研究的讨论[J], 地震学报,1998,18(2):112-118
[123] Dieterich, J H. Time-dependent friction and the mechanics of stick-slip. Pure Appl Geoplys, 1978,116:790-806
[124] Yang Xun-ren, Xie Jin-lai. Detection and analysis of the infrasonic wave attached to the tragic explosion of U.S. space shuttle “Challenger”, Journal of Low Frequency Noise and Vibration, 1986,5(3):100-103
[125] 韩元杰,李绍文,陈宏. 北部湾中小尺度灾害性强对流天气系统的次声/电磁波预警系统的研制与机理探讨. 高原气象,2000,19(2):224-234
[126] 苏昉,田维. 4-8 级大风的次声波三维动态频谱测量研究,石油大学学报,202,26(1):8-11
[127] Karing W, Ludwik L. Burning oil field as a source of infrasound waves. J Low Freq Noise Vib, 1991,10(4):123-129
[128] 梁振福,黄锋,郭丰涛. 艇用次声发声器声场特征及其对艇员的影响. 中国职业医学, 2002,12(6):8-13
[129] 黄志强,梁振福,史秀凤. 扫雷次声场对艇员心理的影响,中华劳动卫生职业病杂志,2003,21(1):27-29
[130] 谢金来. 99 国际次声专家研讨会. 声学学报,2000,01,95-96
[131] Barbara Starr. Non-lethal weapon puzzle for US Army. Internation defense review. 1993,4:319-320
[132] 李爱玲,闫夷升. 次声波及其应用. 现代物理知识,2001,6:33-34
[133] 朱序来,王广献. 次声武器. 外国军事学术,1994,8:57-59
[134] Yount G, Taft R, West J. Possible influence of infrasound on glioma cell response to chemotherapy: a pilot study, J Altern Complement Med, 2004 Apr;10(2):247-50
[135] 严宏,陈景藻,贾克勇. 次声对α-晶体蛋白分子伴侣的活性的影响, 第四军医大学学报,2002,23:1071-73
[136] Svidovyi VI, Shleikin AG. Effect of infrasound on tissuesuccinate dehydrogenase activity, Gig Tr Prof Zabol, 1987,5:50-2
[137] Filatov VV. Experimental study of infrasonic phonophoresis Vestn Oftalmol, 2001,117(6):35-37
[138] Poddubnaia OA, Levitskii EF, Beloborodova EI. The effect of thermal-vibration massage on the function of the hepatobiliary system in patients with chronic cholecystisis and opisthorchiasis, Vopr Kurortol Fizioter Lech fiz Kult, 1999,6:19-21
[139] Sidorenko EI, Obrubov SA, Tumasian AR. Experience of clinical use infrasound pneumomassage in the treatment of progressive myopia in schoolchildren. Vestn Oftalmol, 1997,113(3):18-20
[140] Sidorenko EI, Obrubov SA, Dreval AA. Experimental study of morphologic changes in ocular tissue structures after exposure to infrasound pneumomassage. Vestn Oftalmol, 1996,112(3):17-9
[141] Colasante DA, Au FC, Sell HW. Prophylaxis of adhesions with low frequency sound. Surg Gynecol Obstet, 1981,153(3):357-9
[142] 佘继林,孙艳萍.次声治疗小儿厌食症临床疗效分析.中国中医药信息杂志.1995,2(11):22。
[143] 顾涵森,林厚省.探测气功“运气疗法”物理基础的初步实验结果.自然杂志,1978,1-3
[144] Dimitri MD. Infrasound Method for Bone Mass Measurements.133rd ASA Meeting,state College,PA.June 19,1997
[145] Aripinar P, Simsek B, Sezgin OC, et al. Correlation between mechanical vibration analysis and dual energy X-rayabsorptiometry (DXA) in the measurement of in vivo human tibial bone strength. Technol Health Care. 2005; 13(2):107～113
[146] Jee Wss, In:Weiss L. The skeletal tissues . Cell and Tissue Bilolgy. Munich: Urban and Schwarzenberg, 1998.231～253.
[147]Ryder K D,Duncan R L. Parathyroid hormone modulates the response of osteoblast-like cells to mechanical stimulation . Calcif Tissue Int,2000,67:241～246
[148]申华,陈国振,王建国,等. 振动应力促进骨重建最佳频率的生物力学实验研究. 中华医学杂志,2000,80(10):795～796
[149]. Chao Eys,Inoue N. Enhancement of fracture hearing by mechanical and surgical intervention. Clin Orthop,1998,355:163～178
[150] Bosco C, Colli R, Introini E, et al. Adaptive responses of human skeletal muscle to vibration exposure. Clin Physiol 1999;19:183～187.
[151] Bosco C, Iacovelli M, Tsarpela O, et al. Hormonal responses to whole-body vibration in men. Eur J Appl Physiol 2000;81:449～454.
[152] Torvinen S, Kannus P, Sievanen H, et al. Effect of a vibration exposure on muscular performance and body balance. Randomized cross-over study. Clin Physiol Funct Imaging 2002;22:145～152.
[153] Torvinen S, Kannus P, Sievanen H, et al. Effect of 8-month vertical whole body vibration on bone, muscle performance, and body balance: a randomized controlled study. J Bone MinerRes 2003;18:876～884.
[154] De Ruiter CJ, Van Der Linden RM, Van Der Zijden MJ, et al. Short-term effects of whole-body vibration on maximal voluntary isometric knee extensor force and rate of force rise. Eur J Appl Physiol 2003;88:472～475.
[155] Rubin C, Turner AS, Bain S, Mallinckrodt C, McLeod K. Anabolism: Low mechanical signals strengthen long bones. Nature 2001;412:603～604.
[156] Verschueren SMP, Roelants M, Delecluse C, et al. Effect of 6-month whole body vibration training on hip density, muscle strength, and postural control in postmenopausal women: a randomized controlled pilot study. J Bone Miner Res 2004;19:352～359.
[157] Cardinale M,Wakeling J. Whole body vibration exercise: are vibrations good for you? Br J Sports Med. 2005 Sep;39(9):585～589.
[158] Van Nes IJ, Geurts AC, Hendricks HT, et al. Short-term effects of whole-body vibration on postural control in unilateral chronic stroke patients: preliminary evidence. Am J Phys Med Rehabil 2004;83:867～873.
[159] Crevenna R, Fialka-Moser V, R?dler S, et al. Safety of whole body vibration exercise for heart transplant recipients. Physikalische Medizin Rehabilitation medizin Kurortmedizin 2003;13:286～290.
[160] Iwamoto J, Takeda T, Sato Y, et al. Effect of whole-body vibration exercise on lumbar bone mineral density, boneturnover, and chronic back pain in post-menopausal osteoporotic women treated with alendronate. Aging Clin Exp Res. 2005;17(2):157-63.
[161] 毛勇,段小红,王忠义,等. 不同应力对成骨细胞和细胞骨架影响的实验研究. 牙体牙髓牙周病学杂志,2001,11(2):98～100.
[162] Pavlin D, Zadro R. Temproal pattern of stimulation of osteoblast-associated genes during mechanically-in-duced osteogenesis in vivo: early response of osteocalin and type I collagen. Connect Tissue Res, 2001, 42 (2):135～148.
[163] Pavlin D, Dove SB, Zadro R, et al. Mechanical loading stimulates differentiation of periodontal osteoblasts in ba mouse osteoinduction model: effect on type I collagen and alkaline phosphatase genes. Calcif Tissue Int, 2000, 67( 2):163～172.
[164] Kaspa D, Seidl W, Neilinger, et al. Dynamic cell stretching increases human osteoblast proliferation and CICP synthesis but decrease osteocalin synthesis and alkaline phosphatase activety. Biomech, 2000, 33(1):45～51.
[165] Nagatomi J, Arulanandam BP, Metzger DW, et al. Frequency and duration-dependent effects of cyclic pressure on select bone cell functions. Tissue Eng 2001, 7 ( 6):717～728.
[166] Kaspar D, Seidl W, Claes L, et al. Cyclic dynamic strain effects proliferation and metabolic activity of human osteoblast. 12th Conference of the European Society of Biomechanics, Dublin. 2000. 50～51.
[167] 杨红,罗颂椒. 机械压力对大鼠下颌髁突软骨细胞增殖活性影响的体外研究. 华西口腔医学杂志,1999,11(17):331～333.
[168] Ozawa H,Imamura K,Abe E,Takahashi N,et al. Effect of continuously applied compressive pressure on mouse osteoblast-like cells ( MC3T3-E1 ) in vitro [J]. J cell Physiol,1990,142(1):177-185
[169] Klein-Nulend J,Veldhuijzen JP,de Jong M. Increased bone formation and decreased bone resorption in fetal mouse calvaria as a result of intermittent compressive force in vitro[J]. Bone Miner,1987,2(6):441～448
[170] 王红兵,卢晓,王远亮,等. 拉伸作用对成骨细胞粘附、铺张、粘弹性的影响. 生物物理学报,2001, 17 (3):568～573.
[171] Cillo JE, Gassner R, Koepsel RR, et al. Growth factor and cytokine gene expression In mechanically strained human osteoblast-like cells: Implicaltions for distraction osteogenesis. Oral Sure Oral Med Oral Pathol Oral Radiol Endod, 2000, 90(2):147～154.
[172] Kletsas, Dimitris, basdra, et al. Effect of proteinkinase Inhibitor on the stretch-elicited c-fos and c-jun up-regulation In human PDL osteoblast-like cells. Cell Physiol, 2002, 190(3):313～321.
[173] Peverali FA, Basdra EK, Papaveassiliou AG, et al. Stretch-medated activation of selective MAPK subtypes and potentiation of AP-1 binding In human osteoblastic cells.Mol Med,2001,7(1):68～78.
[174] Ikegame M, Ishibashi O, Yoshizawa T, et al. Tensile stress Induces bone morphogenetic protein-4 in preosteoblastic andfibroblastic cells, which later differentiate into osteoblasts leading to osteogenesis in the mouse calvariae in organ culture. Bone Miner Res, 2001,16(1):24～32.
[175] Akhouayri O, Lafage MH, Rattner A, et al. Effect of static or dynamic mechanical stresses on osteoblast phenotype expression in three-dimensional contractile collagen gels. Cellular biochemistry, 1999, 76: 217～230.
[176] 唐丽灵, 王远亮,潘君,等. 细胞结构的张力完整性. 生物化学与生物物理进展,2001, 28(2) : 160～163.
[177] 冯兵,王德文,何作石,等. G 蛋自在细胞机械感受机制中的作用. 生物化学与生物物理进展,2000, 27 (5):465～468.
[178] 张舒,吴兴裕,李荣辉,等.力学信号在骨骼中的传导. 航人医学与医学工程,2001, 14( 6) : 465～468.
[179] 陈东,王连唐,陈国栋,陈子仪. 去卵巢后大鼠不同部位的骨组织计量学与骨密度研究. 中国骨质疏松杂志. 2002;8(3):208～210
[180] 韩利华,候的门,王全平,等.卵巢切除大鼠皮质骨骨密度与生物力学特性.第四军医大学学报,2001,22;1023～1035.
[181] 李青南,主编.骨质疏松实验动物研究一骨组织形态计量学,成都:四川大学出版社,2001,41～43.
[182] osteoporotic fractures in men: a clinical perspective.Baillieres Best Pract Res Clin Endocrinol Metab,2000,14:299～3 15.
[183] Vandenput L Ederveen AG,Erben RG,et a1.Testosterone pre-vents orchidectomy induced bone loss in estrogen receptor—alpha knockout mice.Biochem Biophys ResCommun.2001.285:70～76.
[184] 陈艳,吴铁崔,燎胡彬. 骨形态计量学观察睾酮对雄性去势大鼠皮质骨的影响. 解剖学研究. 2004,26(4):277～278
[185] 金世鑫.男性骨质疏松概况.中国骨质疏松杂志,2000,6:67～73.
[186] 梁晓萍,董少红,肖学吕,等.增龄及去睾丸大鼠骨密度和性激素因子的实验研究.中国骨质疏松杂志,2002,8: 2ll～2l3.
[187] Frost HM. The Utah paradigm of skeletal physiology: an overview of its insights for bone, cartilage and collagenous tissue organs. J. Bone Miner. Metab. 2000, 18(6):305～316
[188] 侯晋,段小红,吴军正,汪卫国. 持续性静压力对成骨样细胞MC3T3-E1 生物学特性的影响. 临床口腔医学杂志,2004,20(1):9～11.
[189] Ozawa H,Imamura K,Abe E,Takahashi N,et al. Effect of continuously applied compressive pressure on mouse osteoblast-like cells(MC3T3-E1)in vitro. J cell Physiol,1990,142(1):177-185
[190] Klein-Nulend J,Veldhuijzen JP,de Jong M. Increased bone formation and decreased bone resorption in fetal mouse calvaria as a result of intermittent compressive force in vitro. Bone Miner,1987,2(6):441～448
[191] Takahashi M,Chernin MI,yamarnoto O. Transformation of MC3T3-E1 cells following stress and transfection with pSV2neo plasmid. Anticancer Res,2002,22(2A):585～598
[192] Ducy P,Karsenty G. Principles of bone biology. California Academic press,San Diego (1996)Bilezikian J P,Raisz LG,and Rodan G A,eds. 1996,183-190
[193] 万玉民,马永洁,张晓铀,曾彬,王红晖,李莹辉. 回转对离体大鼠成骨细胞中骨粘连蛋白及骨桥素 mRNA 的影响 生理学报 Acta Physiologica Sinica,June 25,2005,57(3):384—388
[194] Martini K, Opltova L. Human nonspecific response to sound stimulation. J Hyg Epidemiol Microbiol Imunol, 1986;30:139-144
[195] Stamenovic D, Fredberg J, Wang N, et al. a microstructural approach to cytoskeletal mechanics based on tensigrity J Theor Biol, 1996,181(2):125-136
[196] Malyankar UM, Scatena M, Suchland KL, et al. Osteoprotegerin is an ανβ3-induced NF-κB-dependent survival factor for endothelial cells. J Biol Chem, 2000,275:20595～20562.
[197] Denhardt DT, Noda M. OPN expression and function: role in bone remodeling. J cell Biochem, 1998,30-31:92～102.
[198] Kaartinen MT, Pirhonen A, Linnala-Kankkunen A, et al. Cross-linking of OPN by tissue transglutaminase increases its collagen binding properties. J Biol Chem, 1999,274:1729～1735.
[199] Toma CD,Ashkar S,Grey ML,et al.Signal transduction of mechanical stimuli is dependent on microfilament integrity:identification of osteopontin as a me chanically induced gene in osteoblast.J Bone Miner Res 1997;l2(6):1626?1636.
[200] 付崇建,杨连甲,张立藩. 模拟失重大鼠股骨和骨髓中骨桥素的变化. 第四军医大学学报,2002;23(3):220～222.
[201] Wang N, Butler JP, Ingber DE. Mechanotransduction across thecell surface and through the cytoskeleton. Science, 1993,260(5111):1124-1127.
[202] Mikuni-Takagaki Y. Mechanical responses and signal transduction pathways in stretched osteocytes. J Bone Miner Metab. 1999;17(1):57-60.
[203] Duncan RL, Turner CH. Mechanotransduction and the functional response of bone to mechanical strain. Calcif Tissue Int, 1995 Nov, 57(5):344-358.
[204] 赵启韬,苗俊英. 激光共聚焦显微镜在生物医学研究中的应用,北京生物医学杂志,2003,22(1):52-54.
[205] Sims JR, Karp S, Ingber DE. Altering the cellular mechanical force balance results in ingegrated changes in cell, cytoskeletal and nuclear shape. J Cell Sci, 1992,103(pt4):1215-1222.
[206] Meazzini MC, Toma CD, Schaffer JL, et al. Osteoblast cytoskeletal modulation in response to mechanical strain in vitro. J Orthop Res, 1998, May 16(2):170-180.
[207] Hughes-Fulford M, Lewis ML Effects of microgravity on osteoblast growth activation. Exp Cell Res, 1996, Apr 224(1):103-109.
[208] Franke RO, Grafe M, Schnittler H. et al. Induction of human vascular endothelial stress fibers by fluid shear stress. Nature, 1984,307:648-654
[209] Olkawa K, Sato M, Ohshima N. Time course changes in cytoskeletal structures of cultured endothelial cells exposed to shear stress. Front Med Biol Eng, 1993,5(2):121
[210] 吕学敏,邓廉夫,杨庆铭. 力学刺激对成骨细胞作用机制的研究进展. 北京生物医学工程,2004;23(2):158～160.
[211] Kudo O, Fujikawa Y, Itonaga I, et al. Proinflammatory cytokine (TNFα/ IL-1α) induction of human osteoclast formation. J Pathol. 2002; 198(2):220～227.
[212] Kubota A, Hasegawa K, Suguro T, et al. Tumor necrosis factor-alpha promotes the expression of osteoprotegerin in rheumatoid synovial fibroblasts. J Rheumatol. 2004; 31(3): 426～435.
[2] arstang M Long-distance. low-frequency elephant communication. J Comp Physiol A Neuroethol Sens Neural Behav Physiol, 2004, Oct; 190(10):791-805
[3] 施志远.动物预报地震的奥秘.北京:科学普及出版社,1993.50-51
[4] 李爱玲,闫夷升.次声波及其应用.现代物理知识. 2001.33-34
[5] 苏昉,苏骁.强地震临震预报的——前兆次声测量研究.石油大学学报(自然科学版),2000,24(6):106-114
[6] 谢金来,谢照华.1993 年 7 月 2 日日本北海道地震次声波.声学学报,1996,21(1)55-61
[7] 牟翔.环境次声测量.第四军医大学博士学位论文.2001.28-34
[8] Sand O, Karlsen HE. Detection of infrasound by the Atlantic cod. J Exp Biol, 1986,125:197-204
[9] 王在忠,张作明,郭群.次声的生物作用的研究进展.预防医学情报杂志,2001,17(5):342-344
[10] Karing W, Ludwik L. Burning oil fields as a source of infrasonic waves. J Low Freq Noise Vib, 1991,10(4):123-9
[11] Ерохин ВН.,и др.Реакция рецеп- торных кле- ток спирального органа на действие инф-равука.Шум,вибрац- ияи борьба с ними напроизводстве: Teз.докл.респ.конф.Л.:1979;92-93
[12] Карпова НИ. и T.д. Низкочастот- ные акустические колебания.М:Мед- ицина,1981,189-190
[13] Schulze J, Auer M, Werzi R. Low level radioactivity measurement in support of the CTBTO. Comprehensive Nuclear-Test –Ban Treaty Organization. Appl Radiat Isot, 2000,53(1-2):23-30
[14] 符文琛.工业次声的劳动卫生问题.职业医学,1985,12(2):47-49
[15] Hennrik M. Annoyance of audilbe infrasound. J Low Freq Noise Vib, 1987,6(1):1-17
[16] Backteman O,Kohler J, Sjoberg L. Infrasound-tutorial and review:part4. Low Freq Noise Vib, 1984,3(1):28-36
[17] 陈景藻.次声的产生及生物学效应.国外医学物理医学与康复分册,1999,19:15-17
[18] 范建中.超声波的物理学基础.见:陈景藻主编.现代物理治疗学.北京:人民军医出版社,2001,326
[19] Broner N. The effects of low frequence noise on people. Areie. Sound and Vibration, 1978,58(4):483-488
[20] Rice CG. Annoyance due to low frequence hums. British Medical Journal, 1994,30895:355.
[21] 苏昉,田维.2001 年 2-4 月强地震的前兆次声波测量研究. 地球物理学进展,2001,16(4):61-70
[22] Georges T M. HF Doppler studies of traveling ionospheric disturbances. J Atmos Terr Phys. 1968,30:735741
[23] 杜功焕,朱哲民,龚秀分.声学基础(上册)上海:上海科学技术出版社,1981,136-138
[24] Gavreau V. infrasound: Generateurs, Detecteurs. Proprietes physiques, effects biologigues. Acustica, 1966,17:1-3
[25] 屠焰,赵树卿.奇异的次声.北京:科学出版社,1979:3-4
[26] Batanov GV. Characteristics of etology of immediate hypersensitivity in condition of exposure to infrasound. Radiat Biol Radioecol, 1995,35(1):78-82
[27] 王冰水,陈景藻,郭国祯,等. 次声作用对 CHO 细胞膜影响的原子力显微镜观察,中华物理医学与康复杂志,2005,27(3):156-158
[28] 丘萍,温宁,姜勇,陈景藻.次声的物理存在及研究意义.中华预防医学杂志,2003,37(10):56-58
[29] 杜宝东,刘淑芳. 次声对人体及动物影响的研究进展.国外医学耳鼻咽喉科学分册,2001,25(2):99-102
[30] 王斌,陈景藻,易南.不同声强 8Hz 次声对小鼠学习能力的影响.第四军医大学学报,1997,18(5):442-445
[31] 魏智钧,李玲,陈景藻. 次声作用对大鼠记忆功能及隔内侧核和斜角带核胆碱能神经元表达的影响.中华物理医学与康复杂志,2001;23(2):83-85
[32] Janhunen, H.K. Combined Effects of Occupational Exposure. Helsinki, finland. Infrasound, 1984,134-139
[33] 费舟,章翔,王小峰.次声作用后鼠脑超微结构与血脑屏障改变,中华物理医学与康复杂志,1999,21(3):134-137
[34] 费舟,章翔关,李树合.次声对脑组织血栓素 A2、前列环素代谢的影响.中华物理医学与康复杂志,2000,22(5):270-272
[35] 刘卫,陈景藻,李玲,刘静,裘明途. 次声对兔脑电活动的影响. 中华物理医学与康复杂志,2002,24(5):269-271
[36] 叶琳,龚书明,黄晓峰,王枫,刘静,陈景藻.次声作用对大鼠大脑皮层超微结构的影响.第四军医大学学报,2002,23(9):856-858
[37] Takigawa. H. Effects of infrasound on vestibular function. J Low Freq Noise and Vib, 1991,151(3):4553
[38] Fu Guo-you, Chen Jing-zao, Jia Ke-Yong. Changes of Glutamate in brain of rats exposed to infrasound. J. Fourth Mil. Med. Univ, 1999. 20(4): 288-290
[39] Liu,Z.H., Chen,J.Z., Tang,Y., et al., Effects of infrasound on changes of intercellular Calcium ion concentration and on expression of RyRs in hippocampus of rat brain. J Low Freq Noise Vib,2005,23(3): 159～165.
[40] 赵乃坤.次声对动物生物学效应的研究.中华劳动卫生职业病杂志,1993,11(6):338
[41] 邢晓辉,王凌,陈景藻,饶志仁. 次声刺激诱导大鼠延髓内儿茶酚胺能神经元 FOS 的表达解放军医学杂志,1998,23(3):189-191
[42] Landstrom U. Laboratory and field studies on infrasound and its effects on human. J Low Frequeqency Noise and Vibration, 1987,6:29-34
[43] Izmerov Nf, Suvorov GA, Kuralesin NA, et al. Infrasound: bodys effects and hygienic regulation. Vestn Ross Akad Med Nauk, 1997,(7):39-46
[44] Kuralesin NA. Hygienic and biomedical aspects of the effects of infrasound. Med Tr Prom Ekol, 1997,(5):8-14
[45] 袁华,陈景藻,李玲. 8Hz 次声作用后大鼠脑热休克蛋白 70 的表达. 中华物理医学与康复杂志,1999,21(2):94-97
[46] 黄文晋,贾克勇,张萍,肖华胜,韩良辅,陈镔复,陈景藻,鞠躬.次声作用后大鼠下丘脑 HSP70 的表达.第四军医大学学报,2000,21(2):162-163
[47] 牟翔,陈景藻,李玲,邱建勇,贾克勇. 次声作用对小鼠脑中胶质纤维酸性蛋白表达和分布的影响.中华物理医学与康复杂志,2001,23(2):76-79
[48] 袁华,龙华,李玲,牟翔,邱建勇,刘静,陈景藻.次声作用后大鼠延髓星形胶质细胞和神经元反应的关系.中国康复理论与实践,2002,8(11):661-663
[49] 裴朝辉.次声对心血管的影响及机制的实验研究,第四军医大学博士学位论文,2004:69-80
[50] Lehoux S, Tedgui A. Shear and signal transduction in the endothelial cell Med Sci. 2004 ,20(5):551-6
[51] McCue S, Noria S, Langille BL. Shear-induced reorganization of endothelial cell cytoskeleton and adhesion complexes. Trends Cardiovasc Med. 2004,14(4):143-51
[52] 裴兆辉,陈景藻,朱妙章.次声诱导的大鼠心肌细胞凋亡和管内皮损伤,第四军医大学学报,2004,25(2):97-99
[53] 陈景藻.次声的存在及其基本生物效应和研究意义.中华物理医学与康复杂志, 1999, 21(3):131-133
[54] Нехорошев АС. Морфофункцио- нальные изменения в миокарде при действии инфраэвука Гиг и Сан 1991; 12: 56
[55] Kanelo S, Okumura K, Numaguchi Y. Melatonin scavenges hydroxyl radical and protects isolated rat hearts from ischemic reperfusion injury. Life Sci, 2000, 67:101-102
[56] 裴兆辉,朱妙章,陈景藻.8Hz,130dB 次声引起心肌组织超微结构的变化. 第四军大学学报,2004;25(11):991-993
[57] Nekhoroshev AS, Glinchikov VV. Morphofunctional changes in the myocardium under exposure to infrasound. NVB. Noise Vib. Bull, 1998,(12):56-58
[58] Gordelaze AS, Glinchilov VV, Usenko VR. Experimental myocardial ischemia caused by infrasound. Gig. Tr.Prof. Zabol, 1986,(6):30-33
[59] 田时秀,李金锡,胡冰.次声对人血压和脉搏的影响. 声学学报,1996,21(3):254-258
[60] 汤家骥.次声的损伤效应,解放军医学情报,1995,9(5):262-264
[61] Kuralesin NA. Hygienic and biomedical aspects of the effects of infrasound. Med Tr Prom Ekol, 1997,(5):8-14
[62] 冯勃,姜泗长,杨伟炎等.强次声波对豚鼠前庭终器超微结构造成的损伤. 中华航空航天医学, 2001,12(2):99-102
[63] Salt A.N., Demott. J.E. Longitudinal endolymph movements and endocochlear potential changes induced by stimulation of infrasound frequencies. J.acoust. Soc. AM, 1999,106(2):847-856
[64] Нехорошев АС.Механизм эффекта при действии инфразвука.Косм-Биол-Авиаком Мед,1990;24(6):39-41
[65] Нехорошев АС.Изучение механи- зма дейст-вия низкочастотных аус- тических колеба нии.Медицина труда и промышленная экология,1998;(5):26-29
[66] Yoshida A, R.Nagano. The influence of infrasound vibration upon cochlea. Sangyo Ika Daigaku Zasshi, 1986,8(suppl.):123-128
[67] 张术,黄维国,邱建华等.次声对豚鼠听阈的影响. 陕西医学杂志, 1998,27(4):253-254
[68] 刑晓辉,陈景藻. 次声刺激诱导大鼠听中枢 FOS 蛋白的表达. 中华物理医学杂志,1998;20(3):165-167
[69] Carricondo F, Bartolome MV, Vicente-Torres MA, et al. Sensitivity to glutamate neurotoxicity in different developmental periods of the rat cochlea. Adv Otorhinolaryngol 2002;59:91-5
[70] Fei zhou, Zhang Xiang, Lu Peiling et al. The changes and significance of cerebral ultrastructure and the expression of heat shock protein 70 in cortex after infrasonic damage. J Low Freq Noise Vib 2000;19(2):93-99
[71] 刘恩渝,章翔,费舟等. 次声作用下大鼠脑皮质内谷氨酸及其代谢型受体 I 亚型 mRNA 的变化. 第四军医大学学报, 2001;22(23):2137-2140
[72] 邢晓辉,贾克勇,陈景藻. 次声刺激后大鼠下丘 n-cNOS 阳性神经元 FOS 蛋白表达,中华劳动卫生职业病杂志,1999,17(4):214-216
[73] 韩良辅,肖华胜陈景藻。次声作用引起大鼠下丘脑 c-fos mRNA 与CRFmRNA 的表达,第四军医大学学报。1999;20(1):1-3
[74] 王冰水. 博士学位论文,第四军医大学. 西安,2005,60-72
[75] 王在忠,张作明,王冰,郭群,李莉. 次声作用后大鼠视网膜组织中 GFAP 与 VEGF 的表达.眼外伤职业病杂志,2002,24(1):6-8
[76] 曹燕,邱萍,惠延年. 次声作用对大鼠血视网膜屏障超微结构的损伤. 2003,19(1):48
[77] 邱萍,张作明,姜勇,郭群,王冰,苟琳,陈景藻.次声暴露对大鼠血视网膜屏障超微结构的损害.中华眼科杂志 2002,38(8):499-501
[78] 王志平,王永建,王荣芬. 未来的超常规武器. 北京,解放军出版社,1985,64
[79] Sidorenko EI, Obrubov SA, Dreval AA, et al. Experiental study of morphologic changes in ocular tissue structures after exposure to infrasound pneumomassage. Vestn Oftalmol, 1996,112:17-19.
[80] 苟琳,张作明,郭守一,贾克勇,邱萍,李莉,郭群,陈景藻. 次声作用对大鼠视网膜 Muller 细胞及血管内皮细胞增殖力的影响,中华航空航天医学杂志,2002,13(1):44-46
[81] 王在忠、张作明,王冰,郭群。次声对大鼠视觉电生理和视网膜超微结构的影响. 眼外伤职业病杂志,2001,23(6):614-616
[82] 石力. 硕士学位论文,第四军医大学,西安,2003,5
[83] 韩凤华,武毅军,陈景藻,黄晓峰,刘小玲,王喜青. 次声下大鼠肝细胞损伤作用的超微结构变化. 西南国防医药, 2003,13(5):465-467
[84] Нехорошев АС. Реакция гема- тоцитов на воздействие инфразв- ука, Гиг и Сан, 1991; 2 :45-47
[85] Lekseev, S.S., V.V. Glinchikov, V.R. Usenko. Reaction of liver cells to infrasound. Gig. Tr. Prof. Zabol, 1986,(9):131-132
[86].Nekhorohev As, Glinchikov VV, Reaction of hepatocytes toinfrasound exposure. Gig Sanit, 1991Feb:2:45-47
[87] Nekhoroshex AS, Glinchikov VV. Morphological research on the liver structures of experimental animals under the action of infrasound. Aviakosm Elolog Med. 1992,26(3):56-9
[88] Ekhoroshev As, Glinchikov VV. Morphological research on the liver structures of experimental animals under the action of infrasound Aviakosm Ekolog Med 1992,May-jun; 26(3):56-59
[89] .Branco N, Santos J, Monteiro E, Silva A, Ferreira J, Pereira M. The lung parenchyma in low frequency noise exposed Wistar rats. Rev Port Pneumol. 2004 Jan-Feb;10(1):77-85
[90] 李唯,贾克勇,李焕章,倪殿涛,陈景藻. 次声对大鼠肺组织损伤及肺氧合功能的影响. 中华劳动卫生职业病杂志.2001,19(3):194-197
[91] 李唯,白汝芬,倪殿涛,李焕章,陈景藻. 8Hz 和 16Hz 单次次声作用后大鼠肺组织匀浆中细胞因子 TNF-α及 IL-8 的变化. 中国急救医学,2002,22(3):127-129
[92] 崔芳,任雨笙,李玲,张荣庆,袁华,贾克勇,陈景藻. 不同声强8Hz 次声作用对大鼠 GSH-PX,SOD 活性及 MDA 含量的影响. 第四军医大学学报, 1999,20(9):743-4
[93] 魏亚宁,刘静,舒青等.次声暴露对小鼠睾丸细胞超微结构的影响,中华男科学,2002,8(5):323-325
[94] 魏亚宁,刘静,舒青等.次声暴露对小鼠卵巢细胞超微结构的影响,第四军医大学学报,2003,24(4):293-295
[95] 刘秀敏,甄荣,陈惠芳等. 次声对小鼠生育能力与免疫功能的影响,中华物理医学与康复杂志,2000,22(2):100-102
[96] Naoko N, Masanobu M, Yasukiyo Y, et al. Process and emergenceon the effects of infrasonic and low frequency noise on inhabitants. J Low Freq Noise Vib, 1989,8:87-99
[97] Nishimura K, Kuroda M, Yoshida Y et al. The pituitary adrenocortical response in rats and human subjects exposed to infrasound. J Low Freq Noise Vib, 1987,(6):18
[98] 赵志刚,陈景藻,张李燕. 次声作用后大鼠血浆中血管紧张素 II含量的改变. 中华物理医学与康复杂志,2001,23(2):83-85
[99] 赵乃坤. 次声对动物生物学效应的研究. 中华劳动卫生职业病杂志, 1993;11(6):338
[100] Колмаков ВН,Свидовый ВИ,Шлейкин АГ. Влияние низкочастотных ак-устических колебаний на некото- рые компоненты мембраны э р и т р о - ц и т о в . Г и г . Т р у да.1984;(10):48-49
[101] 张建保,贾克勇,李玲,五南林,杨继庆,巨宏博,陈景藻. 次声对大鼠血液流变特性的影响,第四军医大学学报,2001;22(5):465-7
[102] Wolff W,White A A,Panjabi M M,et al. Comparision of cyclic loading versus contant compression in the fracture in habbits . Bone and Jiont Surg(Am),1981,63:805～810
[103] Svidovyi VI, Kitaeva LV. Evaluation of cytogenetic activity in bone marrow cells exposed to infrasound (experimental data), Med Tr Prom Ekol, 1998,5:42-4
[104] 范云,杨春智. 次声(物理学教材),第四军医大学, 1995,3
[105] Osamn Okai. Effects of infrasound on respiratory functionof man. J Low Freq Noise and Vib, 1986;5(3):94-99
[106] Svidovyi VI , Kolmakov VN, Kuleba VA, Timofeeva VM. Change in the permeability and total ATPase activity of erythrocytes and blood plasma superoxide dismutase activity on exposure to infrasound in vitr.o. Gig Sanit. 1987May;(5):78-79
[107] Martini K, Opltova L. Human nonspecific response to sound stimulation. J Hyg Epidemiol Mivrobiol Immunol, 1986;30(2):139-44
[108] Idovyi VI, Kuklina OI. State of the hemolymph circulatory bed of conjunctiva as affected by infrasound. Gig Tr Prof Zabol, 1985;Jun(6):51-52
[109] 范建中.超声波的物理学基础.见:陈景藻主编,现代物理治疗学. 北京: 人民军医出版社,2001,328
[110] Balunov VD, Barsukov AF, Artamonova VG. Clinical and functional evaluation of health status of workers exposed to infrasound, noise and general vibration. Med Tr Prom Ekol. 1998;(5):22-26
[111] 黄其柏. 家用电器次声及其声辐射特征的研究. 噪声与振动控制,1997,17(5):6-8
[112] Pawlaczyk LM. Evaluation of occupational exposure to infrasonic noise in Poland. Int J Occup Med Environ Health. 1999;12(2):159-76
[113] Pawlaczyk LM, Augustynska D, Kczmarska KA, Sliwinska KM, Kamedula M. Revised maximum admissible intensity (MAI) values for infrasonic noise in work environment. Med Pr.2001;52(2):119-23
[114] Takahashi Y, Yonekawa Y, Kanada K, Maeda S. An infrasound experiment system for industrial hygiene. Ind Health. 1997 Oct;35(4):480-8
[115] 梁振福,黄锋,唐志文等. 护耳器对次声防护效果的观察. 航天医学与医学工程,2003,16(4):110-114
[116] 汤家骥. 次声武器杀伤因素的生物效应及防护. 人民军医,1995,426(5):5-6
[117] 王斌,陈景藻,易南. 次声对小鼠学习记忆能力的影响及防护. 航空军医,1997,25(3):142-143
[118] 刘思渝,费舟,章翔,陈景藻,刘先珍. 次声作用下大鼠脑皮质I、II 组代谢性谷氨酸受体 mRNA 表达及意义,中华物理医学与康复杂志,2001,23(5):284-287
[119] 李志刚,费舟,章翔,贾克勇,陈景藻,贺晓生,刘先珍. 次声作用后鼠脑CA1区mGluR1α表达改变及其拮抗剂MCPG的作用研究。解放军医学杂志,200126(2):104-106
[120] 张元菊,陈景藻,杨俊峰,王斌,刘静. 16Hz、130dB 次声对小鼠记忆功能和脑超微结构的影响及姜黄素的治疗作用研究,中华物理医学与康复杂志,2005,27(3):152-155
[121] Nekhoshev As, Glinchilov VV. Mechanism of the effect of infrasound on labybrinthine recetors. Kosm Biol Ariakosm Med, 1990,24(6):39-42
[122] 焦明若,张国民. 地震前兆复杂性成因机理研究的讨论[J], 地震学报,1998,18(2):112-118
[123] Dieterich, J H. Time-dependent friction and the mechanics of stick-slip. Pure Appl Geoplys, 1978,116:790-806
[124] Yang Xun-ren, Xie Jin-lai. Detection and analysis of the infrasonic wave attached to the tragic explosion of U.S. space shuttle “Challenger”, Journal of Low Frequency Noise and Vibration, 1986,5(3):100-103
[125] 韩元杰,李绍文,陈宏. 北部湾中小尺度灾害性强对流天气系统的次声/电磁波预警系统的研制与机理探讨. 高原气象,2000,19(2):224-234
[126] 苏昉,田维. 4-8 级大风的次声波三维动态频谱测量研究,石油大学学报,202,26(1):8-11
[127] Karing W, Ludwik L. Burning oil field as a source of infrasound waves. J Low Freq Noise Vib, 1991,10(4):123-129
[128] 梁振福,黄锋,郭丰涛. 艇用次声发声器声场特征及其对艇员的影响. 中国职业医学, 2002,12(6):8-13
[129] 黄志强,梁振福,史秀凤. 扫雷次声场对艇员心理的影响,中华劳动卫生职业病杂志,2003,21(1):27-29
[130] 谢金来. 99 国际次声专家研讨会. 声学学报,2000,01,95-96
[131] Barbara Starr. Non-lethal weapon puzzle for US Army. Internation defense review. 1993,4:319-320
[132] 李爱玲,闫夷升. 次声波及其应用. 现代物理知识,2001,6:33-34
[133] 朱序来,王广献. 次声武器. 外国军事学术,1994,8:57-59
[134] Yount G, Taft R, West J. Possible influence of infrasound on glioma cell response to chemotherapy: a pilot study, J Altern Complement Med, 2004 Apr;10(2):247-50
[135] 严宏,陈景藻,贾克勇. 次声对α-晶体蛋白分子伴侣的活性的影响, 第四军医大学学报,2002,23:1071-73
[136] Svidovyi VI, Shleikin AG. Effect of infrasound on tissuesuccinate dehydrogenase activity, Gig Tr Prof Zabol, 1987,5:50-2
[137] Filatov VV. Experimental study of infrasonic phonophoresis Vestn Oftalmol, 2001,117(6):35-37
[138] Poddubnaia OA, Levitskii EF, Beloborodova EI. The effect of thermal-vibration massage on the function of the hepatobiliary system in patients with chronic cholecystisis and opisthorchiasis, Vopr Kurortol Fizioter Lech fiz Kult, 1999,6:19-21
[139] Sidorenko EI, Obrubov SA, Tumasian AR. Experience of clinical use infrasound pneumomassage in the treatment of progressive myopia in schoolchildren. Vestn Oftalmol, 1997,113(3):18-20
[140] Sidorenko EI, Obrubov SA, Dreval AA. Experimental study of morphologic changes in ocular tissue structures after exposure to infrasound pneumomassage. Vestn Oftalmol, 1996,112(3):17-9
[141] Colasante DA, Au FC, Sell HW. Prophylaxis of adhesions with low frequency sound. Surg Gynecol Obstet, 1981,153(3):357-9
[142] 佘继林,孙艳萍.次声治疗小儿厌食症临床疗效分析.中国中医药信息杂志.1995,2(11):22。
[143] 顾涵森,林厚省.探测气功“运气疗法”物理基础的初步实验结果.自然杂志,1978,1-3
[144] Dimitri MD. Infrasound Method for Bone Mass Measurements.133rd ASA Meeting,state College,PA.June 19,1997
[145] Aripinar P, Simsek B, Sezgin OC, et al. Correlation between mechanical vibration analysis and dual energy X-rayabsorptiometry (DXA) in the measurement of in vivo human tibial bone strength. Technol Health Care. 2005; 13(2):107～113
[146] Jee Wss, In:Weiss L. The skeletal tissues . Cell and Tissue Bilolgy. Munich: Urban and Schwarzenberg, 1998.231～253.
[147]Ryder K D,Duncan R L. Parathyroid hormone modulates the response of osteoblast-like cells to mechanical stimulation . Calcif Tissue Int,2000,67:241～246
[148]申华,陈国振,王建国,等. 振动应力促进骨重建最佳频率的生物力学实验研究. 中华医学杂志,2000,80(10):795～796
[149]. Chao Eys,Inoue N. Enhancement of fracture hearing by mechanical and surgical intervention. Clin Orthop,1998,355:163～178
[150] Bosco C, Colli R, Introini E, et al. Adaptive responses of human skeletal muscle to vibration exposure. Clin Physiol 1999;19:183～187.
[151] Bosco C, Iacovelli M, Tsarpela O, et al. Hormonal responses to whole-body vibration in men. Eur J Appl Physiol 2000;81:449～454.
[152] Torvinen S, Kannus P, Sievanen H, et al. Effect of a vibration exposure on muscular performance and body balance. Randomized cross-over study. Clin Physiol Funct Imaging 2002;22:145～152.
[153] Torvinen S, Kannus P, Sievanen H, et al. Effect of 8-month vertical whole body vibration on bone, muscle performance, and body balance: a randomized controlled study. J Bone MinerRes 2003;18:876～884.
[154] De Ruiter CJ, Van Der Linden RM, Van Der Zijden MJ, et al. Short-term effects of whole-body vibration on maximal voluntary isometric knee extensor force and rate of force rise. Eur J Appl Physiol 2003;88:472～475.
[155] Rubin C, Turner AS, Bain S, Mallinckrodt C, McLeod K. Anabolism: Low mechanical signals strengthen long bones. Nature 2001;412:603～604.
[156] Verschueren SMP, Roelants M, Delecluse C, et al. Effect of 6-month whole body vibration training on hip density, muscle strength, and postural control in postmenopausal women: a randomized controlled pilot study. J Bone Miner Res 2004;19:352～359.
[157] Cardinale M,Wakeling J. Whole body vibration exercise: are vibrations good for you? Br J Sports Med. 2005 Sep;39(9):585～589.
[158] Van Nes IJ, Geurts AC, Hendricks HT, et al. Short-term effects of whole-body vibration on postural control in unilateral chronic stroke patients: preliminary evidence. Am J Phys Med Rehabil 2004;83:867～873.
[159] Crevenna R, Fialka-Moser V, R?dler S, et al. Safety of whole body vibration exercise for heart transplant recipients. Physikalische Medizin Rehabilitation medizin Kurortmedizin 2003;13:286～290.
[160] Iwamoto J, Takeda T, Sato Y, et al. Effect of whole-body vibration exercise on lumbar bone mineral density, boneturnover, and chronic back pain in post-menopausal osteoporotic women treated with alendronate. Aging Clin Exp Res. 2005;17(2):157-63.
[161] 毛勇,段小红,王忠义,等. 不同应力对成骨细胞和细胞骨架影响的实验研究. 牙体牙髓牙周病学杂志,2001,11(2):98～100.
[162] Pavlin D, Zadro R. Temproal pattern of stimulation of osteoblast-associated genes during mechanically-in-duced osteogenesis in vivo: early response of osteocalin and type I collagen. Connect Tissue Res, 2001, 42 (2):135～148.
[163] Pavlin D, Dove SB, Zadro R, et al. Mechanical loading stimulates differentiation of periodontal osteoblasts in ba mouse osteoinduction model: effect on type I collagen and alkaline phosphatase genes. Calcif Tissue Int, 2000, 67( 2):163～172.
[164] Kaspa D, Seidl W, Neilinger, et al. Dynamic cell stretching increases human osteoblast proliferation and CICP synthesis but decrease osteocalin synthesis and alkaline phosphatase activety. Biomech, 2000, 33(1):45～51.
[165] Nagatomi J, Arulanandam BP, Metzger DW, et al. Frequency and duration-dependent effects of cyclic pressure on select bone cell functions. Tissue Eng 2001, 7 ( 6):717～728.
[166] Kaspar D, Seidl W, Claes L, et al. Cyclic dynamic strain effects proliferation and metabolic activity of human osteoblast. 12th Conference of the European Society of Biomechanics, Dublin. 2000. 50～51.
[167] 杨红,罗颂椒. 机械压力对大鼠下颌髁突软骨细胞增殖活性影响的体外研究. 华西口腔医学杂志,1999,11(17):331～333.
[168] Ozawa H,Imamura K,Abe E,Takahashi N,et al. Effect of continuously applied compressive pressure on mouse osteoblast-like cells ( MC3T3-E1 ) in vitro [J]. J cell Physiol,1990,142(1):177-185
[169] Klein-Nulend J,Veldhuijzen JP,de Jong M. Increased bone formation and decreased bone resorption in fetal mouse calvaria as a result of intermittent compressive force in vitro[J]. Bone Miner,1987,2(6):441～448
[170] 王红兵,卢晓,王远亮,等. 拉伸作用对成骨细胞粘附、铺张、粘弹性的影响. 生物物理学报,2001, 17 (3):568～573.
[171] Cillo JE, Gassner R, Koepsel RR, et al. Growth factor and cytokine gene expression In mechanically strained human osteoblast-like cells: Implicaltions for distraction osteogenesis. Oral Sure Oral Med Oral Pathol Oral Radiol Endod, 2000, 90(2):147～154.
[172] Kletsas, Dimitris, basdra, et al. Effect of proteinkinase Inhibitor on the stretch-elicited c-fos and c-jun up-regulation In human PDL osteoblast-like cells. Cell Physiol, 2002, 190(3):313～321.
[173] Peverali FA, Basdra EK, Papaveassiliou AG, et al. Stretch-medated activation of selective MAPK subtypes and potentiation of AP-1 binding In human osteoblastic cells.Mol Med,2001,7(1):68～78.
[174] Ikegame M, Ishibashi O, Yoshizawa T, et al. Tensile stress Induces bone morphogenetic protein-4 in preosteoblastic andfibroblastic cells, which later differentiate into osteoblasts leading to osteogenesis in the mouse calvariae in organ culture. Bone Miner Res, 2001,16(1):24～32.
[175] Akhouayri O, Lafage MH, Rattner A, et al. Effect of static or dynamic mechanical stresses on osteoblast phenotype expression in three-dimensional contractile collagen gels. Cellular biochemistry, 1999, 76: 217～230.
[176] 唐丽灵, 王远亮,潘君,等. 细胞结构的张力完整性. 生物化学与生物物理进展,2001, 28(2) : 160～163.
[177] 冯兵,王德文,何作石,等. G 蛋自在细胞机械感受机制中的作用. 生物化学与生物物理进展,2000, 27 (5):465～468.
[178] 张舒,吴兴裕,李荣辉,等.力学信号在骨骼中的传导. 航人医学与医学工程,2001, 14( 6) : 465～468.
[179] 陈东,王连唐,陈国栋,陈子仪. 去卵巢后大鼠不同部位的骨组织计量学与骨密度研究. 中国骨质疏松杂志. 2002;8(3):208～210
[180] 韩利华,候的门,王全平,等.卵巢切除大鼠皮质骨骨密度与生物力学特性.第四军医大学学报,2001,22;1023～1035.
[181] 李青南,主编.骨质疏松实验动物研究一骨组织形态计量学,成都:四川大学出版社,2001,41～43.
[182] osteoporotic fractures in men: a clinical perspective.Baillieres Best Pract Res Clin Endocrinol Metab,2000,14:299～3 15.
[183] Vandenput L Ederveen AG,Erben RG,et a1.Testosterone pre-vents orchidectomy induced bone loss in estrogen receptor—alpha knockout mice.Biochem Biophys ResCommun.2001.285:70～76.
[184] 陈艳,吴铁崔,燎胡彬. 骨形态计量学观察睾酮对雄性去势大鼠皮质骨的影响. 解剖学研究. 2004,26(4):277～278
[185] 金世鑫.男性骨质疏松概况.中国骨质疏松杂志,2000,6:67～73.
[186] 梁晓萍,董少红,肖学吕,等.增龄及去睾丸大鼠骨密度和性激素因子的实验研究.中国骨质疏松杂志,2002,8: 2ll～2l3.
[187] Frost HM. The Utah paradigm of skeletal physiology: an overview of its insights for bone, cartilage and collagenous tissue organs. J. Bone Miner. Metab. 2000, 18(6):305～316
[188] 侯晋,段小红,吴军正,汪卫国. 持续性静压力对成骨样细胞MC3T3-E1 生物学特性的影响. 临床口腔医学杂志,2004,20(1):9～11.
[189] Ozawa H,Imamura K,Abe E,Takahashi N,et al. Effect of continuously applied compressive pressure on mouse osteoblast-like cells(MC3T3-E1)in vitro. J cell Physiol,1990,142(1):177-185
[190] Klein-Nulend J,Veldhuijzen JP,de Jong M. Increased bone formation and decreased bone resorption in fetal mouse calvaria as a result of intermittent compressive force in vitro. Bone Miner,1987,2(6):441～448
[191] Takahashi M,Chernin MI,yamarnoto O. Transformation of MC3T3-E1 cells following stress and transfection with pSV2neo plasmid. Anticancer Res,2002,22(2A):585～598
[192] Ducy P,Karsenty G. Principles of bone biology. California Academic press,San Diego (1996)Bilezikian J P,Raisz LG,and Rodan G A,eds. 1996,183-190
[193] 万玉民,马永洁,张晓铀,曾彬,王红晖,李莹辉. 回转对离体大鼠成骨细胞中骨粘连蛋白及骨桥素 mRNA 的影响 生理学报 Acta Physiologica Sinica,June 25,2005,57(3):384—388
[194] Martini K, Opltova L. Human nonspecific response to sound stimulation. J Hyg Epidemiol Microbiol Imunol, 1986;30:139-144
[195] Stamenovic D, Fredberg J, Wang N, et al. a microstructural approach to cytoskeletal mechanics based on tensigrity J Theor Biol, 1996,181(2):125-136
[196] Malyankar UM, Scatena M, Suchland KL, et al. Osteoprotegerin is an ανβ3-induced NF-κB-dependent survival factor for endothelial cells. J Biol Chem, 2000,275:20595～20562.
[197] Denhardt DT, Noda M. OPN expression and function: role in bone remodeling. J cell Biochem, 1998,30-31:92～102.
[198] Kaartinen MT, Pirhonen A, Linnala-Kankkunen A, et al. Cross-linking of OPN by tissue transglutaminase increases its collagen binding properties. J Biol Chem, 1999,274:1729～1735.
[199] Toma CD,Ashkar S,Grey ML,et al.Signal transduction of mechanical stimuli is dependent on microfilament integrity:identification of osteopontin as a me chanically induced gene in osteoblast.J Bone Miner Res 1997;l2(6):1626?1636.
[200] 付崇建,杨连甲,张立藩. 模拟失重大鼠股骨和骨髓中骨桥素的变化. 第四军医大学学报,2002;23(3):220～222.
[201] Wang N, Butler JP, Ingber DE. Mechanotransduction across thecell surface and through the cytoskeleton. Science, 1993,260(5111):1124-1127.
[202] Mikuni-Takagaki Y. Mechanical responses and signal transduction pathways in stretched osteocytes. J Bone Miner Metab. 1999;17(1):57-60.
[203] Duncan RL, Turner CH. Mechanotransduction and the functional response of bone to mechanical strain. Calcif Tissue Int, 1995 Nov, 57(5):344-358.
[204] 赵启韬,苗俊英. 激光共聚焦显微镜在生物医学研究中的应用,北京生物医学杂志,2003,22(1):52-54.
[205] Sims JR, Karp S, Ingber DE. Altering the cellular mechanical force balance results in ingegrated changes in cell, cytoskeletal and nuclear shape. J Cell Sci, 1992,103(pt4):1215-1222.
[206] Meazzini MC, Toma CD, Schaffer JL, et al. Osteoblast cytoskeletal modulation in response to mechanical strain in vitro. J Orthop Res, 1998, May 16(2):170-180.
[207] Hughes-Fulford M, Lewis ML Effects of microgravity on osteoblast growth activation. Exp Cell Res, 1996, Apr 224(1):103-109.
[208] Franke RO, Grafe M, Schnittler H. et al. Induction of human vascular endothelial stress fibers by fluid shear stress. Nature, 1984,307:648-654
[209] Olkawa K, Sato M, Ohshima N. Time course changes in cytoskeletal structures of cultured endothelial cells exposed to shear stress. Front Med Biol Eng, 1993,5(2):121
[210] 吕学敏,邓廉夫,杨庆铭. 力学刺激对成骨细胞作用机制的研究进展. 北京生物医学工程,2004;23(2):158～160.
[211] Kudo O, Fujikawa Y, Itonaga I, et al. Proinflammatory cytokine (TNFα/ IL-1α) induction of human osteoclast formation. J Pathol. 2002; 198(2):220～227.
[212] Kubota A, Hasegawa K, Suguro T, et al. Tumor necrosis factor-alpha promotes the expression of osteoprotegerin in rheumatoid synovial fibroblasts. J Rheumatol. 2004; 31(3): 426～435.