紫菜遗传多样性分析及其6-磷酸海藻糖合成酶基因转化水稻的研究
|
摘要
用分子标记技术进行紫菜遗传多样性分析对于紫菜种质资源的鉴定、保护和持续合理开发利用具有重要意义。本研究采用相关序列扩增多态性(sequence-related amplified polymorphism,SRAP);靶位区域扩增多态性(target region amplification polymorphism,TRAP);限制性位点扩增多态性(restriction site amplification polymorphism,RSAP)三种分子标记技术对16个紫菜系进行了遗传多样性分析,在此基础上对生产上广泛应用的条斑紫菜6-磷酸海藻糖合成酶基因(trehalose-6-phosphate synthase from Porphyra yezoensis,PyTPS )进行了转化水稻的研究。
本研究首次将分子标记技术SRAP、TRAP和RSAP用于紫菜的种质资源分析。建立了适合于紫菜SRAP、TRAP和RSAP分析的PCR反应体系和反应条件,利用所建立的体系对16个紫菜系进行了遗传多样性分析,构建了这些紫菜系的聚类图和DNA指纹图。三种分子标记方法聚类分析所产生的进化树基本一致,均把这些紫菜系分成两大类,分类结果与传统分类相吻合。在所构建的DNA指纹图谱中,每个紫菜系都有其特异的指纹模式,能很容易地与其它紫菜系区分开来。根据DNA指纹图谱开发出计算机应用软件PGI-SRAP,PGI-TRAP,PGI-RSAP(Porphyra germplasm identification developed by SRAP,TRAP and RSAP method),可以辅助进行紫菜系的种质鉴定。对RSAP分析产生的17条特异性标记中的6条进行了克隆、测序,并将其中一个标记R1/R3-8119转换成紫菜系P. yezoensis Y-9101的特异序列扩增区域(sequence characterized amplified region,SCAR)标记。
用本实验室克隆的条斑紫菜TPS基因(PyTPS)转化水稻品种TP309,得到的T0代转基因植株,经过连续加代、鉴定、筛选,得到5个T2代的纯合体株系,对其中3个株系(TPS155-4,TPS191-1和TPS308-1)进行了PCR和Southern杂交检测,结果都呈双重阳性,表明PyTPS基因已整合到这些转基因株系中。用0.8%NaCl模拟盐碱条件,16% PEG-6000模拟干旱条件,以非转基因水稻TP309为对照,选T2代两个纯合体株系TPS155-4和TPS191-1进行了抗盐、抗旱性分析。RT-PCR结果表明转入的基因得以表达,转基因株系的株高、单株鲜重均高于对照,在PEG处理下这种差异达到显著水平。这说明PyTPS基因的导入,明显增加了转基因水稻的抗旱性;转基因水稻的抗盐性也有所提高。
It is very important to study the genetic diversity of Porphyra lines using molecular marker technique, which could promote the germplasm identification, protection and effectual exploiture of Porphyra. The genetic diversity of Porphyra lines was analysed using molecular marker techniques sequence-related amplified polymorphism (SRAP), target region amplification polymorphism (TRAP) and restriction site amplified polymorphism (RSAP). And the trehalose-6-phosphate synthase gene from Porphyra yezoensis (PyTPS) cloned by our laboratory was transformed to rice variety TP309, in order to study the function of the gene PyTPS.
SRAP, TRAP and RSAP analytic system were set up and successfully used in germplasm identification of Porphyra lines in this study firstly. The dendrograms and the DNA fingerprints of sixteen Porphyra lines were constructed according to the result of SRAP, TRAP and RSAP analysis. The dendrograms from 3 marker techniques were accordant with the conventional taxonomy basically. In the DNA fingerprints, each of the 16 Porphyra lines has its unique fingerprinting pattern and can be easily distinguished from each other. The PGI-SRAP, PGI-TRAP and PGI-RSAP (Porphyra germplasm identification developed by SRAP, TRAP and RSAP method) developed by DNA fingerprints could be used to germplasm identification of these Porphyra lines. In addition, seventeen specific RSAP markers were identified and six of them were sequenced. One of the 17 specific markers, R1/R3-8119 from P. yezoensis Y-9101, was successfully converted into sequence characterized amplification region (SCAR) marker.
Trehalose-6-phosphate synthase gene from P. yezoensis (PyTPS) cloned by our laboratory was transformed into rice variety TP309 by Agrobacterium mediated method. Three T_2 homozygous lines were obtained by the selection with kanamycin resistance, PCR amplification and Southern blotting analysis. Results indicated that the PyTPS gene had been integrated into the genome of the transgenic rice plants. The tolerance to both salt and drought was compared between two homozygous lines TPS155-4, TPS191-1 and the non-transformed rice plants TP309, imposed 0.8% NaCl and 16% PEG-6000 treatments. The plant height and fresh weight per plant in transgenic plants were better than those of control plants under water, salt and drought stress. The difference between transgenic and control plants for drought stress was obvious. The result demonstrated that the transgenic plants showed apparently increased drought stress tolerance and the slightly increased salt stress tolerance.
本研究首次将分子标记技术SRAP、TRAP和RSAP用于紫菜的种质资源分析。建立了适合于紫菜SRAP、TRAP和RSAP分析的PCR反应体系和反应条件,利用所建立的体系对16个紫菜系进行了遗传多样性分析,构建了这些紫菜系的聚类图和DNA指纹图。三种分子标记方法聚类分析所产生的进化树基本一致,均把这些紫菜系分成两大类,分类结果与传统分类相吻合。在所构建的DNA指纹图谱中,每个紫菜系都有其特异的指纹模式,能很容易地与其它紫菜系区分开来。根据DNA指纹图谱开发出计算机应用软件PGI-SRAP,PGI-TRAP,PGI-RSAP(Porphyra germplasm identification developed by SRAP,TRAP and RSAP method),可以辅助进行紫菜系的种质鉴定。对RSAP分析产生的17条特异性标记中的6条进行了克隆、测序,并将其中一个标记R1/R3-8119转换成紫菜系P. yezoensis Y-9101的特异序列扩增区域(sequence characterized amplified region,SCAR)标记。
用本实验室克隆的条斑紫菜TPS基因(PyTPS)转化水稻品种TP309,得到的T0代转基因植株,经过连续加代、鉴定、筛选,得到5个T2代的纯合体株系,对其中3个株系(TPS155-4,TPS191-1和TPS308-1)进行了PCR和Southern杂交检测,结果都呈双重阳性,表明PyTPS基因已整合到这些转基因株系中。用0.8%NaCl模拟盐碱条件,16% PEG-6000模拟干旱条件,以非转基因水稻TP309为对照,选T2代两个纯合体株系TPS155-4和TPS191-1进行了抗盐、抗旱性分析。RT-PCR结果表明转入的基因得以表达,转基因株系的株高、单株鲜重均高于对照,在PEG处理下这种差异达到显著水平。这说明PyTPS基因的导入,明显增加了转基因水稻的抗旱性;转基因水稻的抗盐性也有所提高。
It is very important to study the genetic diversity of Porphyra lines using molecular marker technique, which could promote the germplasm identification, protection and effectual exploiture of Porphyra. The genetic diversity of Porphyra lines was analysed using molecular marker techniques sequence-related amplified polymorphism (SRAP), target region amplification polymorphism (TRAP) and restriction site amplified polymorphism (RSAP). And the trehalose-6-phosphate synthase gene from Porphyra yezoensis (PyTPS) cloned by our laboratory was transformed to rice variety TP309, in order to study the function of the gene PyTPS.
SRAP, TRAP and RSAP analytic system were set up and successfully used in germplasm identification of Porphyra lines in this study firstly. The dendrograms and the DNA fingerprints of sixteen Porphyra lines were constructed according to the result of SRAP, TRAP and RSAP analysis. The dendrograms from 3 marker techniques were accordant with the conventional taxonomy basically. In the DNA fingerprints, each of the 16 Porphyra lines has its unique fingerprinting pattern and can be easily distinguished from each other. The PGI-SRAP, PGI-TRAP and PGI-RSAP (Porphyra germplasm identification developed by SRAP, TRAP and RSAP method) developed by DNA fingerprints could be used to germplasm identification of these Porphyra lines. In addition, seventeen specific RSAP markers were identified and six of them were sequenced. One of the 17 specific markers, R1/R3-8119 from P. yezoensis Y-9101, was successfully converted into sequence characterized amplification region (SCAR) marker.
Trehalose-6-phosphate synthase gene from P. yezoensis (PyTPS) cloned by our laboratory was transformed into rice variety TP309 by Agrobacterium mediated method. Three T_2 homozygous lines were obtained by the selection with kanamycin resistance, PCR amplification and Southern blotting analysis. Results indicated that the PyTPS gene had been integrated into the genome of the transgenic rice plants. The tolerance to both salt and drought was compared between two homozygous lines TPS155-4, TPS191-1 and the non-transformed rice plants TP309, imposed 0.8% NaCl and 16% PEG-6000 treatments. The plant height and fresh weight per plant in transgenic plants were better than those of control plants under water, salt and drought stress. The difference between transgenic and control plants for drought stress was obvious. The result demonstrated that the transgenic plants showed apparently increased drought stress tolerance and the slightly increased salt stress tolerance.
引文
陈一华,贾建航,李传友等。通过AFLP-DNA指纹的计算机分析进行水稻种子鉴定。农业生物技术学报,2000a, 8 (3): 222-224
陈一华,张超良,王泽立等。玉米种子的DNA指纹计算机化鉴定。应用与环境生物学报,2000b, 6 (3): 223-226
崔灵英,许璞,朱建一等。4种紫菜叶状体的ISSR分子标记分析。中国水产科学,2006, 13 (3): 371-377
戴继勋。用细胞工程技术发展我国的紫菜养殖业。生物工程进展,2000, 20 (6): 3-4, 8
戴继勋。用海藻细胞工程技术发展我国的海水养殖业。世界科技研究与发展,2002a, 24 (3): 28-31
戴继勋。大型海藻细胞工程研究与应用.中国科学技术前沿[M]。北京:高等教育出版社, 2002b, 5: 695-722
戴秀玉,吴大鹏,周坚。大肠杆菌海藻糖合成酶基因的克隆和表达。遗传学报,2000, 27: 158-164.
杜晓华,王得元,巩振辉。一种新型DNA标记技术—限制性位点扩增多态性(RSAP)的建立与优化。西北农林科技大学学报, 2006, 34 (9): 45-50
范晓,张士璀,秦松等。海洋生物技术新进展[M]。北京海洋出版社,2000
贾建航,李传友,金德敏等。AFLP技术在玉米自交系类群分析研究中的应用。高技术通讯,1999, 9 (4): 43-47
贾建航,陈一华,石金峰等。用于紫菜无性与种质鉴定的计算机DNA指纹的建立。海洋学报,2001, 3 (1): 79-83
孔凡真。保健佳品:紫菜。中国保健食品,2006, 9: 23
林忠旭,张献龙,聂以春等。棉花SRAP遗传连锁图构建。科学通报, 2003, 15: 90-93
林忠旭,张献龙,聂以春。新型标记SRAP在棉花F2分离群体及遗传多样性评价中的适应性分析。遗传学报,2004, 31 (6): 622-626
刘必谦,曾庆国,骆其君等。条斑紫菜(Porphyra yezoensis) dbEST中筛选微卫星位点及引物种间转移扩增。海洋与湖沼,2005a, 6 (3): 248-254
刘必谦,曾国庆,骆其军等。微卫星标记在坛紫菜丝状体品系DNA指纹构建中的应用。水产学报,2005b, 29 (3): 323-326
刘杨,陈火英,魏毓棠等。番茄SSR遗传连锁图谱的构建及几个产量相关性状QTLs的定位。自然科学进展,2005, 15 (6): 748-752
刘宇峰,徐力敏,张成武等。红藻藻蓝蛋白对HL-60细胞生长的抑制作用。中国海洋药物,2000, 19: 20-24.
柳波,孙彬,金德敏等。紫菜自由丝状体SCAR标记的获得.高技术通讯,2004, 12: 88-92
罗钰如,曾呈奎。当代中国的海洋事业[M]。中国社会科学出版社,1985
梅俊学,金德敏,贾建航等。条斑紫菜不同栽培品系的RAPD研究。山东大学学报(自然科学版),2000, 35 (2): 230-234
沈利爽,何平,徐云碧等。水稻DH群体的分子连锁图谱及基因组分析。植物学报, 1998, 40 (12): 1115-1122
石金锋,贾建航,王萍等。紫菜无性系特异分子标记的获得。高技术通讯,2000, 10: 1-3
石金锋,贾建航,金德敏等。紫菜无性系特异SCAR标记的获得。海洋学报,2003, 25 (1): 28-32
石运庆,牟秋换,李鹏等。DNA分子标记及其在作物育种中的应用。山东科学,2005, 18 (2): 22-29
汤晓荣,费修绠。紫菜叶状体发育研究进展。青岛海洋大学学报,2000, 30 (2): 183-190
唐启升。海洋生物技术研究发展与展望。海洋科学,1999, 1: 33-35
王斌,翁曼丽。AFLP的原理及其应用。杂交水稻,1996, 5: 27~30
王斌。分子标记及其应用[A]。植物细胞工程与分子育种技术研究[C],陆维忠,郑企成主编。北京,中国农业科学技术出版社, 2003, 167-181
王娟,戴继勋,张义听等。紫菜的生殖与生活史研究进展。中国水产科学,2006, 13 (2): 322-327
王娟,戴继勋,张义听等。紫菜减数分裂的研究现状及展望.中国海洋大学学报,2006, 36 (3): 377-380
王青山,李葱葱,王晶等。AFLP分子标记技术及应用研究进展。吉林农业科学, 2005, 30 (6): 29-33
王忆琴,戴秀玉,王韫询等。大肠杆菌OtsA基因的克隆和表达。微生物学报,2000, (5): 270-274
王自章,张树珍,杨本鹏等。甘蔗根癌农杆菌介导转化海藻糖合酶基因获得抗渗透胁迫能力增强植株。中国农业科学,2003, 36 (2): 140-146
翁曼丽,谢纬武,伏健民等。新一代的分子标记技术—AFLP。应用与环境生物学报,1996, 2(4): 424-429
吴亮其,范战民,郭蕾等。通过转基因δ-OAT基因获得抗盐抗旱水稻。科学通报,2003, 48(19): 2050-2056
伍华菊,张建平,夏安东等。条斑紫菜中R-藻红蛋白的生化特性。生物化学与生物物理学报,1994, 26: 491-497
徐涤,宋林生,秦松等。五个紫菜品系间遗传差异的RAPD分析。高技术通讯,2001, 12: 1-3
许绍斌,陶玉芬,杨昭庆等。简单快速的DNA银染和胶保存方法。遗传,2002, 24 (3): 335-336
严小军,秦松,曾呈奎。藻类分子生物技术两年评——与藻类活性物质研究有关的生物技术. 海洋与湖沼,1997, 28 (4): 440-446
闫华超,高岚,李桂兰。分子标记技术的发展及应用。生物学通报,2006, 41(2): 17-19
杨锐,刘必谦,骆其君等。利用扩增片段长度多态性(AFLP)研究坛紫菜的遗传变异。高技术通讯,2002, 12 (1): 83-86
杨锐,刘必谦,骆其君等。利用AFLP技术研究条斑紫菜的遗传变异。海洋学报,2005, 27 (3): 159-162
杨宇峰,费修绠。大型海藻对富营养化海水养殖区生物修复的研究与展望。青岛海洋大学学报,2003, 33 (1): 53-57
曾呈奎等。中国经济海藻志[M].北京:科学出版社, 1962, 10
曾繁杰,林启山,蒋丽金等。红藻坛紫菜中R-藻蓝蛋白的分离和特性。生物化学与生物物理学报,1992, 24: 545-552
张树珍,郑学勤。基因枪介导甘蔗遗传转化研究初报。热带作物学报,2001, 22 (1): 35-41
张学成,秦松,马家海等。海藻遗传学[M]。中国农业出版社,2004 赵恢武,陈杨坚,胡莺雷等。干旱诱导性启动子驱动的海藻糖-6-磷酸合酶基因载体的构建及转基因烟草的耐早性。植物学报,2000, 42 (6): 616-619
周慧萍,陈琼华。紫菜多糖抗衰老作用的实验研究。中国药科大学学报,1989, 20: 231-234
周慧萍,陈琼华。紫菜多糖的抗凝血和降血脂作用。中国药科大学学报,1990, 21: 358-360 Aisaka K., Masuda T., Chikamune T., et al. Purification and characterization of trehalose phosphorylase from Catellatospora ferruginea. Biosci. Biotechnol. Biochem., 1998, 62 (4): 782-787
Andersson U., Levander F. and Radstrom P. Trehalose-6-phosphate phosphorylase is part of a novel metabolic pathway for trehalose utilization in Lactococcus lactis. J.Biol.Chem. 2001, 276 (46):42707-41713
Arguelles J.C. Physiological roles of trehalose in bacteria and yeasts: a comparative analysis. Arch Microbiol., 2000, 174 (4): 217-224
Asamizu E, Nakajima M, Kitade Y. et al. Comparison of RNA expression profiles between the two generations of Porphyra yezoensis (Rhodophyta), based on expressed sequence tag frequency analysis. J. Phycol., 2003, 39 (5): 923-930
Bell W., Sun W., and Hohmann S. Composition and functional analysis of the Saccharomyces cerevisiae trehalose synthase complex. J. Biol. Chem.1998, 273 (50): 33311-33319
Blazquez M. and Santos E. Isolation and molecular characterization of the Arabidopsis TPS1 gene encoding trehalose-6-phosphate synthase. Plant J. 1998, 13: 685-690
Boos W, Ehmann U, Bremer E, et al. Trehalase of Escherichia coli. Mapping and cloning of its structural gene and identification of the enzyme as a periplasmic protein induced under high osmolarity growth conditions. J. Biol. Chem., 1987, 262 (27): 13212-13218.
Botstein D., White R.L., Skolnick M. et al. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American journal of human genetics, 1980, 32 (3): 314–331
Budak H., Shearman R.C., Parmaksiz I., et al. Molecular characterization of buffalograss germplasm using sequence-related amplified polymorphism markers. Theor. Appl. Genet. 2004, 108: 328–334
Burger G, Saint-Louis D, Gray M.W. Complete sequence of the mitochondrial DNA of the red alga Porphyra purpurea. Cyanobacterial introns and shared ancestry of red and green alga. Plant Cell 1999, 11: 1675-1694
Cabib E. and Leloir L. The biosynthesis of trehalose phosphate. J. Biol. Chem. 1958, 231: 259-275
Chen J.F., Hu J.G., Vick B.A. et al.. Molecular mapping of a nuclear male-sterility gene in sunflower ( Helianthus annuus L.) using TRAP and SSR markers. Theor. Appl. Genet. 2006, 113: 122–127
Chen Y.H., Jia J.H., Li C.Y. et al. Rice seed identification by computerized AFLP-DNA fingerprinting. Chinese rice research newsletter, 2000, 8 (1): 4-5
Chomcznki P. and Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chlorofom extraction. Anal. Biochem. 1987. 162: 156-159
Colaco C. and Sen S.. Extraordinary stability of enzymes dried in trehalose: simplified molecular biology. Bio/Technology, 1992, 10: 1007-1011.
Crowe J. Preservation of membranes in anhydrobiotic organism: the role of trehalose. Science, 1984a, 223: 701-703
Crowe J. and Whittam M. Interactions of phospholipids monolayers with carbohydrates. Biochim. Biophys. Acta, 1984b, 769: 151-159
De Smet K. A., Weston A., Brown I. N., et al. Three pathways for trehalose biosynthesis in mycobacteria. Microbiology, 2002, 146: 199-208
Devirgilio C. The role of trehalose synthesis for the acquisition of thermotolerance in yeast. I. Genetic evidence that trehalose is a thermoprotectant. Eur. J. Biochem. 1994, 219: 179-186
Dirlewanger E., Cosson P., Tavaud M., et al. Development of microsatellite markers in peach [ Prunus persica (L.) Batsch] and their use in genetic diversity analysis in peach and sweet cherry ( Prunus avium L.) Theor. Appl. Genet. 2002, 105: 127–138
Donis-Keller H., Green P., Helms C., et al. A genetic linkage map of the human genome. Cell, 1987, 51 (2): 319-337
Drennan P.M., Smith M.T., Goldsworthy D., et a1. The occurrence of trehalose in the leaves of the desiccation to1errant angiosperm Myrothamnus flabellifolius Welw.Plant Physiol., 1993, 142: 493-496
Dutcher J.A. and Kapmun D.F. Random amplification polymorphic DNA (RAPD) identification of genetic variation in three species of Porphyra (Bangiales,Rhodophyta).J. Appl. Phycol. 1994, 6: 267-273
Eastmond P. and van Dijken A. Trehalose-6-phosphate synthase1, which catalyses the first step in trehalose synthesis, is essential for Arabidopsis embryo maturation. Plant J. 2002, 29: 225-235.
Eis C. and Nidetzky B. Characterization of trehalose phosphorylase from Schizophyllum commune. Biochem J.1999, 341 (2): 385-393
Elbein A. The metabolism ofα,α-trehalose. Adv. Carbohydr.Chem.Biochem. 1974, 30: 227-256
Ferriol M., Pico B., Nuez F. Genetic diversity of a germplasm collection of Cucurbita pepo using SRAP and AFLP markers. Theor. Appl. Genet. 2003a, 107: 271-282
Ferriol M., Pico B., Nuez F. Genetic diversity of some accessions of Oucurbita maxima from Spain using RAPD and SRAP markers. Genet. Res. Crop Evol. 2003b, 50: 227-238
Fleurence J. Seaweedproteins: biochemical, nutritional aspects and potential uses. Trends in Food Technol., 1999, 10: 25-28
Garcia A.and Engler J. Effects of osmoprotectants upon NaCl stress in rice. Plant Physiol.1997, 155: 159-169
Garg A.K., Kim J.K., Thomas G., et a1. Trehalose accumulation in rice plants confers high tolerance levels to difierent abiotie stresses.Proc. Nation. Acad. Sci., 2002, 99 (25): 15898-15903
Goddijn O. and Verwoerd T. Inhibition of trehalase activity enhances trehalose accumulation in transgenic plants. Plant Physiol. 1997, 113: 181-190
Goddijn O. and Smeekens S. Sensing trehalose biosynthesis in plants. Plant J. 1998, 14: 143-146
Goddijn O., and van Dun, K.Trehalose metabolism in plants. Trends in Plant Sci. 1999, 4: 315-319
Gotsche S. and Dahl M.K. Purification and characterization of the phospho-alpha (1,1) glucosidase (TreA) of Bacillus subtilis. J. Bacteriol. 1995, 177 (10): 2721-2726
Hemmat M., Weeden N.F., Manganaris A.G., et al. Molecular marker linkage map for apple. The Journal of heredity, 1994, 85 (1): 4-11
Hu J.G. & Vick B.A. Target Region Amplification Polymorphism:A novel marker technique for plant genotyping. Plant Molecular Biology Reporter 2003, 21: 289-294
Hu J.G., Ochoa O.E., Truco M.J. et al. Application of the TRAP technique to lettuce (Lactuca sativa L.) genotyping. Euphytica 2005,144: 225-235
Holmstrom K.O., Mantyla E., Welin B., et a1. Drought tolerance in tobacco. Nature, 1996, 379: 683-684
Holmstrom K.O. Engineering plant adaptation to waterstress.Acta universitatis Agriculture sueciae Agraia, 1998, 84: 49
Iitsuka O., Nakamura K., Ozaki A., et al. Genetic information of three pure lines of Porphyra yezoensis (Bangiales, Rhodophyta) obtained by AFLP analysis. Fisheries Sci. 2002, 68: 1113-1117
Ineichen K.and Wiemken V. Changes in the fungus-specific, soluble-carbohydrate pool during rapid and synchronous ectomycorrhiza formation of Picea abies with Pisolithus tinctorius. Mycorrhiza.1992, 2: 1-7
Jia J.H., Wang P, Jin D.M., et al. The application of RAPD markers in diversity detection and variety identification of Porphyra. Acta Bota. Sini., 2000, 42 (4): 403-407
Kaasen I., Falkenberg P., Styrvold O.B., et a1. Molecular cloning and physical mapping of the otsBA genes,which encode the osmaregulatory trehalose pathway of Escherichia coli:evidence that transcription is activated by KatF (AppR).J. Bacteriol.1992, 174 (3): 889-898
Kapraun D.F., Hinson T.K., Lemus A.J. Karyology and cytophotometric estimation of Inter- and Intra-specific nuclear DNA variation in four species of Porphyra (Rhodophyta). Phycologia, 1991, 30 (4): 458-466
Kato M, Miura Y, Kettoku M, et al. Purification and characterization of new trehalose-producing enzymes isolated from the hyperthermophilic archae, sulfolobus solfataricus KM1. Biosci Biotechnol. Biochem., 1996, 60 (3): 546-550
Kim Y.H., Kwon T.K., Park S., et al. Trehalose synthesis by sequential reactions of recombinant maltooligosyltrehalose synthase and maltooligosyltrehalose trehalohydrolase from Brevibacterium helvolum. Appl. Environ. Microbiol., 2000, 11: 4620-4624
Lee E.K., Seo S.B., Kim T.H., et al. Analysis of expressed sequence tags of Porphyra yezoensis. Mol. Cells, 2000, 10: 338-342
Leyman B. and Van Dijck, P. An unexpected plethora of trehalose synthesizing genes in Arabidopsis thaliana. Trends Plant Sci.2001,6: 5110-5113
Li G. and Quiros C.F. Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theor. Appl. Genet. 2001, 103: 455-461
Li G., Gao M., Yang B., et al. Gene for gene alignment between the Brassica and Arabidopsis genomes by direct transcriptome mapping. Theor. Appl. Genet. 2003, 107: 168-180
Liu Z.H., Anderson J.A., Hu J.G., et al. A wheat intervarietal genetic linkage map based on microsatellite and target region amplified polymorphism markers and its utility for detecting quantitative trait loci. Theor. Appl. Genet. 2005b, 111: 782–794
Ma J.H. and Cai S.Q. Cultivation and processing of Porphyra yezoensis[M]. Science Press. Beijing, 1996, 1-68
Mackenzie K. and Singh K. Water stress plating hypersensitivity of yeast: protective role of trehalose in Saccharomyces cerevisiae. J.Gen.Microbiol.1988, 134: 1661-1666
Maruta K., Hattori K., Nakada T., et al. Cloning and sequencing of trehalose biosynthesis genesfrom Arthrobacter sp. Q36. Biochim.Biophys.Acta., 1996a, 1289 (1): 10-13
Maruta K, Hattori K, Nakada T, et al. Cloning and sequencing of trehalose biosynthesis genes from Rhizobium sp.M-11. Biosci. Biotech. Biochem.,1996b, 60 (4): 717-720
McCouch S.R., Kochert G., Yu Z.H., et al. Molecular mapping of rice chromosomes. Theor. Appl. Genet. 1988, 76 (6): 815-829
Michelmore R.W., Paran I., Kesseli R.V., Identification of markers linked to disease-resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proc. Nation. Acad. Sci., 1991, 88 (21): 9828-9832
Mitman G.G., van der Meer J.P. Meiosis, blade development and sex determination in Porphyra purpurea (Phodophyta). J. Phycol. 1994, 30: 147-159
Mizukami Y, Okauchi M, Kito H, et a1. Discrimination of laver cultivars with RAPD markers. Fish Sci, 1996, 62 (4): 547-551
Muehlbauer G. J., Specht J. E., Thomas-Compton M. A., et al. Near-isogenic line- a potential resource in the integration of conventional and molecular marker linkage maps. Crop Science, 1988, 28: 729-735
Muller J. and Xie Z.. Trehalose and trehalase in root nodules of various legumes. Plant Physiol. 1994, 90: 86-92
Muller J. and Boller A. Effects of validamycin A, a potent trehalase inhibitor, and phytohormones on trehalose metabolism in roots and root nodules of soybean and cowpea. Planta, 1995, 197: 362-368
Muller J. and Wiemken A. Trehalose metabolism in sugar sensing and plant development. Plant Sci.1999, 147: 37-47
Muller J. and Aeschbacher R. Trehalose and trehalase in Arabidopsis. Plant Physiol. 2001, 125: 1086-1093
Murry H.G., and Thompson W. F. Rapid isolation of high molecular weight plant DNA. Nucl. Acids Res. 1980, 8: 4321-4325
Nakada T. and Maruta, K. Purification and properties of a novel enzyme, maltooligosyl trehalose synthase, from Arthrobacter sp. Q36. Biosci. Biotech. Biochem. 1995, 59: 2210-2214
Nikaido I., Asamizu E., Nakajima M., et al. Generation of 10,154 Expressed Sequence Tags from a leafy gametophyte of a marine red alga, Porphyra yezoensis. DNA Research, 2000, 7: 223-227
Niwa K, Kikuchi N, Iwabuchi M, et al.Morphological and AFLP variation of Porphyra yezoensisUeda form narawaensis miura (Bangiales, Rhodophyta).Phycological Research, 2004, 52 (2): 180-186
Noda H. Health benefits and nutritional properties of nori. J. Appl. Phycol.1993, 5:255-258
Oscar J.M., Goddijn Kees Van Dun. Trehalose Metabolism in Plants. Trends Plant Sci., 1999, 4 (8): 315-319
Otting G. Protein hydrtion in apueomns solution.Science.1991, 254: 974
Paul M. and Pellny T. Enhancing photosynthesis with sugar signals. Trends Plant Sci. 2001, 6: 197-200
Peakall R., Gilmore S., Keys W., et al. Cross-Species Amplification of Soybean (Glycine max) Simple Sequence Repeats (SSRs) Within the Genus and Other Legume Genera: Implications for the Transferability of SSRs in Plants. Mol. Biol. Evol.1998,15 (10): 1275-1287
Philips D. and Wilson D. Soluble carbohydrates in legumes and nodulates nonlegumes. J. Agric. Food Chem.1984, 32: 1289-1291
Reith M. and Munholland J. A high-resolution gene map of the chloroplast genome of the red alga Porphyra purpurea. Plant Cell, 1993, 5: 465-475
Romero C. and Belles J. Expression of the yeast trehalose-6-phosphate synthase gene in transgenic tobacco plants: pleiotropic phenotypes include drought tolerance. Planta, 1997, 201:293-297
Rungis D., Berube Y., Zhang J., et al. Robust simple sequence repeat markers for spruce (Picea spp) from expressed sequence tags. Theor. Appl. Genet. 2004, 109 (6): 1283-1294
Saito K., Yamazaki H., Ohnishi Y., et al. Production of trehalose synthase from abasidkycete. Grifola frondosa,in Eschherichia coil. App1. Microbiol. Biotechnol.1998, 50 (2): 193-198
Sheppard L.J., Franssen I. and Cape J.N. Frost hardiness of Norwayspruce treated with acid mist. Evaluation of the electrolyte leakage rate technique. Environ. Exp. Bot. 1995, 35: 139-149
Song S.H. and Zheng X.Y. Purity determination of Chinese cabbage F1 cultivars with RAPD markers. Acta Agriculturae Boreali-Sinica, 2000, 15 (4): 35-39
Stiller J.W. and Waaland J.R. Molecular analysis reveals cryptic diversity in Porphyra (Rhodophyta). J. Phycol, 1993, 29: 506-517
Sun J,W., Jin D.M., Zhou C.J., et al.Identification of Porphyra lines (Rhodophyta) by AFLP-DNA fingerprinting and molecular markers. Plant Molecular Biology Reporter 2005, 23 (3): 1-12
Sun J.W., Jin D. M., Weng M. L., et al. Development of SSR Primers from EST Sequences and Their Application in Germplasm Identification of Porphyra Lines. J. Europ. Phycol, 2006, 41: 329–336
Thaller M. and Schippa S. Conserved sequence motifs among bacterial, eukaryotic and archaeal phosphatases that define a new phosphohydrolase superfamily. Protein Sci. 1998, 7: 1647-1652
Thevelein J. and Hohmann S. Trehalose synthase: guard to the gate of glycolysis in yeast? Trends Biochem.Sci.1995, 20: 3-10
Tsusaki K., Nishimoto T., Nakada T., et al. Cloning and sequencing of trehalose synthase gene from Pimelobacter sp. R48. Biochim. Biophys. Acta, 1996, 1290 (1): 1-3
Tsusaki K., Nishimoto T., Nakada T, et al. Cloning and sequencing of trehalose synthase gene from Thermus aquaticus ATCC33923. Biochim.Biophys.Acta, 1997, 1334 (1): 28-32.
Van Vaeck C., Wera S., van Dijck P., et al. Analysis and modification of trehalose-6-phosphate levels in the yeast Saccharomyces cerevisiae with the use of Bacillus subtilis phosphotrehalase. Biochem J. 2001, 353: 157-162
Varshneya R.K., Sigmunda R., Bornera A. et al. Interspecific transferability and comparative mapping of barley EST-SSR markers in wheat, rye and rice. Plant Sci. 2005, 168: 195-202.
Vogel G. and Aeschbacher R. Trehalose-6-phosphate phosphatases from Arabidopsis thaliana: identification by functional complementation of the yeast tps2 mutant. Plant J. 1998, 13: 673-683
Vos P., Hogers R., Bleeker M, et a1. AFLP: a new technique for DNA fingerprinting. Nucl. Acids Res.1995, 23 (21): 4407-4414
Waaland J.R., Stiller J.W. and Cheney D.P. Macroalgal candidates for genomics. J. Phycol, 2004, 40 (1): 26-33
Wagner W. and Wiemken, A. Regulation of fructan metabolism in leaves of barley (Hordeum vulgare L. cv Gerbel). Plant Physiol.1986, 81: 444-447
Welsh J and McClelland M. Fingerprinting genomes using PCR with arbitrary primers. Nucl. Acids Res. 1990, 18 (24): 7213-7218
Wang B., Jia J.H., Shi J.F. et al. Identification of Porphyra lines using computerized DNA fingerprinting. Acta Oceanologica Sinica, 2001, 20 (3): 401-407
Wang X.L., Yang Y.X., Cong Y.Z. et al. DNA fingerprinting of selected Laminaria (Phaeophyta) gametophytes by RAPD markers. Aquaculture, 2004, 238: 143~153
Weng M.L., Liu B., Jin D.M. et al. Identification of 27 porphyra lines (Rhodophyta) by DNA fingerprinting and molecular markers. J. Appl. Phycol., 2005, 17 (1): 91-97
Wiemken A. Trehalose in yeast, stress protectant rather than reserve carbohydrate. J. Gen. Microbiol. 1990, 58: 209-217
Williams J.G., Kubelik A.R., Livak K.J., et a1. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.Nucl. Acids Res.1990, 18 (24): 6531-6535
Wingler A. and Fritzius T. Trehalose induced the ADP-glucose pyrophosphorylase gene, ApL3, and starch synthesis in Arabidopsis. Plant Physiol. 2000, 124: 105-114
Yarish C., Wilkes R., Chopin T., et al. Domestication of indigenous Porphyra species for commercial cultivation in Northeast America. World Aquaculture 1998, 29 (4): 26-55
Yeo E.T., Kwon H.B., Han S. E., et a1. Genetic engineering of drought resistant potato plants by introduction of the trehalose-6-phosphonte synthase (TPS1) gene from Saccharomyces cerevisiae. Mol.Cells,2000, 10 : 263-268
Zabeau M. and Vos P. Selective restriction fragment amplification:a general method for DNA fingerprinting [P]. European patient office publication 1993, 0535 858A1
Zhang C.L., Sun Z.L., Jin D.M., et al. Identification of maize inbred lines and validation of genetic relationship among maize inbred lines using RAPD marker. Maize Genetics Cooperation Newsletter, 1998, 72: 9-10
Zhang D., Zhang Z., Yang K., et al. Genetic mapping in (Populus tomentosa x Populus bolleana) and P. tomentosa Carr. using AFLP markers. Theor. Appl. Genet. 2004, 108 (4): 657-662
Zietkiewicz E., Rafalski A. and Labuda D. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics, 1994, 20: 176-183
陈一华,张超良,王泽立等。玉米种子的DNA指纹计算机化鉴定。应用与环境生物学报,2000b, 6 (3): 223-226
崔灵英,许璞,朱建一等。4种紫菜叶状体的ISSR分子标记分析。中国水产科学,2006, 13 (3): 371-377
戴继勋。用细胞工程技术发展我国的紫菜养殖业。生物工程进展,2000, 20 (6): 3-4, 8
戴继勋。用海藻细胞工程技术发展我国的海水养殖业。世界科技研究与发展,2002a, 24 (3): 28-31
戴继勋。大型海藻细胞工程研究与应用.中国科学技术前沿[M]。北京:高等教育出版社, 2002b, 5: 695-722
戴秀玉,吴大鹏,周坚。大肠杆菌海藻糖合成酶基因的克隆和表达。遗传学报,2000, 27: 158-164.
杜晓华,王得元,巩振辉。一种新型DNA标记技术—限制性位点扩增多态性(RSAP)的建立与优化。西北农林科技大学学报, 2006, 34 (9): 45-50
范晓,张士璀,秦松等。海洋生物技术新进展[M]。北京海洋出版社,2000
贾建航,李传友,金德敏等。AFLP技术在玉米自交系类群分析研究中的应用。高技术通讯,1999, 9 (4): 43-47
贾建航,陈一华,石金峰等。用于紫菜无性与种质鉴定的计算机DNA指纹的建立。海洋学报,2001, 3 (1): 79-83
孔凡真。保健佳品:紫菜。中国保健食品,2006, 9: 23
林忠旭,张献龙,聂以春等。棉花SRAP遗传连锁图构建。科学通报, 2003, 15: 90-93
林忠旭,张献龙,聂以春。新型标记SRAP在棉花F2分离群体及遗传多样性评价中的适应性分析。遗传学报,2004, 31 (6): 622-626
刘必谦,曾庆国,骆其君等。条斑紫菜(Porphyra yezoensis) dbEST中筛选微卫星位点及引物种间转移扩增。海洋与湖沼,2005a, 6 (3): 248-254
刘必谦,曾国庆,骆其军等。微卫星标记在坛紫菜丝状体品系DNA指纹构建中的应用。水产学报,2005b, 29 (3): 323-326
刘杨,陈火英,魏毓棠等。番茄SSR遗传连锁图谱的构建及几个产量相关性状QTLs的定位。自然科学进展,2005, 15 (6): 748-752
刘宇峰,徐力敏,张成武等。红藻藻蓝蛋白对HL-60细胞生长的抑制作用。中国海洋药物,2000, 19: 20-24.
柳波,孙彬,金德敏等。紫菜自由丝状体SCAR标记的获得.高技术通讯,2004, 12: 88-92
罗钰如,曾呈奎。当代中国的海洋事业[M]。中国社会科学出版社,1985
梅俊学,金德敏,贾建航等。条斑紫菜不同栽培品系的RAPD研究。山东大学学报(自然科学版),2000, 35 (2): 230-234
沈利爽,何平,徐云碧等。水稻DH群体的分子连锁图谱及基因组分析。植物学报, 1998, 40 (12): 1115-1122
石金锋,贾建航,王萍等。紫菜无性系特异分子标记的获得。高技术通讯,2000, 10: 1-3
石金锋,贾建航,金德敏等。紫菜无性系特异SCAR标记的获得。海洋学报,2003, 25 (1): 28-32
石运庆,牟秋换,李鹏等。DNA分子标记及其在作物育种中的应用。山东科学,2005, 18 (2): 22-29
汤晓荣,费修绠。紫菜叶状体发育研究进展。青岛海洋大学学报,2000, 30 (2): 183-190
唐启升。海洋生物技术研究发展与展望。海洋科学,1999, 1: 33-35
王斌,翁曼丽。AFLP的原理及其应用。杂交水稻,1996, 5: 27~30
王斌。分子标记及其应用[A]。植物细胞工程与分子育种技术研究[C],陆维忠,郑企成主编。北京,中国农业科学技术出版社, 2003, 167-181
王娟,戴继勋,张义听等。紫菜的生殖与生活史研究进展。中国水产科学,2006, 13 (2): 322-327
王娟,戴继勋,张义听等。紫菜减数分裂的研究现状及展望.中国海洋大学学报,2006, 36 (3): 377-380
王青山,李葱葱,王晶等。AFLP分子标记技术及应用研究进展。吉林农业科学, 2005, 30 (6): 29-33
王忆琴,戴秀玉,王韫询等。大肠杆菌OtsA基因的克隆和表达。微生物学报,2000, (5): 270-274
王自章,张树珍,杨本鹏等。甘蔗根癌农杆菌介导转化海藻糖合酶基因获得抗渗透胁迫能力增强植株。中国农业科学,2003, 36 (2): 140-146
翁曼丽,谢纬武,伏健民等。新一代的分子标记技术—AFLP。应用与环境生物学报,1996, 2(4): 424-429
吴亮其,范战民,郭蕾等。通过转基因δ-OAT基因获得抗盐抗旱水稻。科学通报,2003, 48(19): 2050-2056
伍华菊,张建平,夏安东等。条斑紫菜中R-藻红蛋白的生化特性。生物化学与生物物理学报,1994, 26: 491-497
徐涤,宋林生,秦松等。五个紫菜品系间遗传差异的RAPD分析。高技术通讯,2001, 12: 1-3
许绍斌,陶玉芬,杨昭庆等。简单快速的DNA银染和胶保存方法。遗传,2002, 24 (3): 335-336
严小军,秦松,曾呈奎。藻类分子生物技术两年评——与藻类活性物质研究有关的生物技术. 海洋与湖沼,1997, 28 (4): 440-446
闫华超,高岚,李桂兰。分子标记技术的发展及应用。生物学通报,2006, 41(2): 17-19
杨锐,刘必谦,骆其君等。利用扩增片段长度多态性(AFLP)研究坛紫菜的遗传变异。高技术通讯,2002, 12 (1): 83-86
杨锐,刘必谦,骆其君等。利用AFLP技术研究条斑紫菜的遗传变异。海洋学报,2005, 27 (3): 159-162
杨宇峰,费修绠。大型海藻对富营养化海水养殖区生物修复的研究与展望。青岛海洋大学学报,2003, 33 (1): 53-57
曾呈奎等。中国经济海藻志[M].北京:科学出版社, 1962, 10
曾繁杰,林启山,蒋丽金等。红藻坛紫菜中R-藻蓝蛋白的分离和特性。生物化学与生物物理学报,1992, 24: 545-552
张树珍,郑学勤。基因枪介导甘蔗遗传转化研究初报。热带作物学报,2001, 22 (1): 35-41
张学成,秦松,马家海等。海藻遗传学[M]。中国农业出版社,2004 赵恢武,陈杨坚,胡莺雷等。干旱诱导性启动子驱动的海藻糖-6-磷酸合酶基因载体的构建及转基因烟草的耐早性。植物学报,2000, 42 (6): 616-619
周慧萍,陈琼华。紫菜多糖抗衰老作用的实验研究。中国药科大学学报,1989, 20: 231-234
周慧萍,陈琼华。紫菜多糖的抗凝血和降血脂作用。中国药科大学学报,1990, 21: 358-360 Aisaka K., Masuda T., Chikamune T., et al. Purification and characterization of trehalose phosphorylase from Catellatospora ferruginea. Biosci. Biotechnol. Biochem., 1998, 62 (4): 782-787
Andersson U., Levander F. and Radstrom P. Trehalose-6-phosphate phosphorylase is part of a novel metabolic pathway for trehalose utilization in Lactococcus lactis. J.Biol.Chem. 2001, 276 (46):42707-41713
Arguelles J.C. Physiological roles of trehalose in bacteria and yeasts: a comparative analysis. Arch Microbiol., 2000, 174 (4): 217-224
Asamizu E, Nakajima M, Kitade Y. et al. Comparison of RNA expression profiles between the two generations of Porphyra yezoensis (Rhodophyta), based on expressed sequence tag frequency analysis. J. Phycol., 2003, 39 (5): 923-930
Bell W., Sun W., and Hohmann S. Composition and functional analysis of the Saccharomyces cerevisiae trehalose synthase complex. J. Biol. Chem.1998, 273 (50): 33311-33319
Blazquez M. and Santos E. Isolation and molecular characterization of the Arabidopsis TPS1 gene encoding trehalose-6-phosphate synthase. Plant J. 1998, 13: 685-690
Boos W, Ehmann U, Bremer E, et al. Trehalase of Escherichia coli. Mapping and cloning of its structural gene and identification of the enzyme as a periplasmic protein induced under high osmolarity growth conditions. J. Biol. Chem., 1987, 262 (27): 13212-13218.
Botstein D., White R.L., Skolnick M. et al. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American journal of human genetics, 1980, 32 (3): 314–331
Budak H., Shearman R.C., Parmaksiz I., et al. Molecular characterization of buffalograss germplasm using sequence-related amplified polymorphism markers. Theor. Appl. Genet. 2004, 108: 328–334
Burger G, Saint-Louis D, Gray M.W. Complete sequence of the mitochondrial DNA of the red alga Porphyra purpurea. Cyanobacterial introns and shared ancestry of red and green alga. Plant Cell 1999, 11: 1675-1694
Cabib E. and Leloir L. The biosynthesis of trehalose phosphate. J. Biol. Chem. 1958, 231: 259-275
Chen J.F., Hu J.G., Vick B.A. et al.. Molecular mapping of a nuclear male-sterility gene in sunflower ( Helianthus annuus L.) using TRAP and SSR markers. Theor. Appl. Genet. 2006, 113: 122–127
Chen Y.H., Jia J.H., Li C.Y. et al. Rice seed identification by computerized AFLP-DNA fingerprinting. Chinese rice research newsletter, 2000, 8 (1): 4-5
Chomcznki P. and Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chlorofom extraction. Anal. Biochem. 1987. 162: 156-159
Colaco C. and Sen S.. Extraordinary stability of enzymes dried in trehalose: simplified molecular biology. Bio/Technology, 1992, 10: 1007-1011.
Crowe J. Preservation of membranes in anhydrobiotic organism: the role of trehalose. Science, 1984a, 223: 701-703
Crowe J. and Whittam M. Interactions of phospholipids monolayers with carbohydrates. Biochim. Biophys. Acta, 1984b, 769: 151-159
De Smet K. A., Weston A., Brown I. N., et al. Three pathways for trehalose biosynthesis in mycobacteria. Microbiology, 2002, 146: 199-208
Devirgilio C. The role of trehalose synthesis for the acquisition of thermotolerance in yeast. I. Genetic evidence that trehalose is a thermoprotectant. Eur. J. Biochem. 1994, 219: 179-186
Dirlewanger E., Cosson P., Tavaud M., et al. Development of microsatellite markers in peach [ Prunus persica (L.) Batsch] and their use in genetic diversity analysis in peach and sweet cherry ( Prunus avium L.) Theor. Appl. Genet. 2002, 105: 127–138
Donis-Keller H., Green P., Helms C., et al. A genetic linkage map of the human genome. Cell, 1987, 51 (2): 319-337
Drennan P.M., Smith M.T., Goldsworthy D., et a1. The occurrence of trehalose in the leaves of the desiccation to1errant angiosperm Myrothamnus flabellifolius Welw.Plant Physiol., 1993, 142: 493-496
Dutcher J.A. and Kapmun D.F. Random amplification polymorphic DNA (RAPD) identification of genetic variation in three species of Porphyra (Bangiales,Rhodophyta).J. Appl. Phycol. 1994, 6: 267-273
Eastmond P. and van Dijken A. Trehalose-6-phosphate synthase1, which catalyses the first step in trehalose synthesis, is essential for Arabidopsis embryo maturation. Plant J. 2002, 29: 225-235.
Eis C. and Nidetzky B. Characterization of trehalose phosphorylase from Schizophyllum commune. Biochem J.1999, 341 (2): 385-393
Elbein A. The metabolism ofα,α-trehalose. Adv. Carbohydr.Chem.Biochem. 1974, 30: 227-256
Ferriol M., Pico B., Nuez F. Genetic diversity of a germplasm collection of Cucurbita pepo using SRAP and AFLP markers. Theor. Appl. Genet. 2003a, 107: 271-282
Ferriol M., Pico B., Nuez F. Genetic diversity of some accessions of Oucurbita maxima from Spain using RAPD and SRAP markers. Genet. Res. Crop Evol. 2003b, 50: 227-238
Fleurence J. Seaweedproteins: biochemical, nutritional aspects and potential uses. Trends in Food Technol., 1999, 10: 25-28
Garcia A.and Engler J. Effects of osmoprotectants upon NaCl stress in rice. Plant Physiol.1997, 155: 159-169
Garg A.K., Kim J.K., Thomas G., et a1. Trehalose accumulation in rice plants confers high tolerance levels to difierent abiotie stresses.Proc. Nation. Acad. Sci., 2002, 99 (25): 15898-15903
Goddijn O. and Verwoerd T. Inhibition of trehalase activity enhances trehalose accumulation in transgenic plants. Plant Physiol. 1997, 113: 181-190
Goddijn O. and Smeekens S. Sensing trehalose biosynthesis in plants. Plant J. 1998, 14: 143-146
Goddijn O., and van Dun, K.Trehalose metabolism in plants. Trends in Plant Sci. 1999, 4: 315-319
Gotsche S. and Dahl M.K. Purification and characterization of the phospho-alpha (1,1) glucosidase (TreA) of Bacillus subtilis. J. Bacteriol. 1995, 177 (10): 2721-2726
Hemmat M., Weeden N.F., Manganaris A.G., et al. Molecular marker linkage map for apple. The Journal of heredity, 1994, 85 (1): 4-11
Hu J.G. & Vick B.A. Target Region Amplification Polymorphism:A novel marker technique for plant genotyping. Plant Molecular Biology Reporter 2003, 21: 289-294
Hu J.G., Ochoa O.E., Truco M.J. et al. Application of the TRAP technique to lettuce (Lactuca sativa L.) genotyping. Euphytica 2005,144: 225-235
Holmstrom K.O., Mantyla E., Welin B., et a1. Drought tolerance in tobacco. Nature, 1996, 379: 683-684
Holmstrom K.O. Engineering plant adaptation to waterstress.Acta universitatis Agriculture sueciae Agraia, 1998, 84: 49
Iitsuka O., Nakamura K., Ozaki A., et al. Genetic information of three pure lines of Porphyra yezoensis (Bangiales, Rhodophyta) obtained by AFLP analysis. Fisheries Sci. 2002, 68: 1113-1117
Ineichen K.and Wiemken V. Changes in the fungus-specific, soluble-carbohydrate pool during rapid and synchronous ectomycorrhiza formation of Picea abies with Pisolithus tinctorius. Mycorrhiza.1992, 2: 1-7
Jia J.H., Wang P, Jin D.M., et al. The application of RAPD markers in diversity detection and variety identification of Porphyra. Acta Bota. Sini., 2000, 42 (4): 403-407
Kaasen I., Falkenberg P., Styrvold O.B., et a1. Molecular cloning and physical mapping of the otsBA genes,which encode the osmaregulatory trehalose pathway of Escherichia coli:evidence that transcription is activated by KatF (AppR).J. Bacteriol.1992, 174 (3): 889-898
Kapraun D.F., Hinson T.K., Lemus A.J. Karyology and cytophotometric estimation of Inter- and Intra-specific nuclear DNA variation in four species of Porphyra (Rhodophyta). Phycologia, 1991, 30 (4): 458-466
Kato M, Miura Y, Kettoku M, et al. Purification and characterization of new trehalose-producing enzymes isolated from the hyperthermophilic archae, sulfolobus solfataricus KM1. Biosci Biotechnol. Biochem., 1996, 60 (3): 546-550
Kim Y.H., Kwon T.K., Park S., et al. Trehalose synthesis by sequential reactions of recombinant maltooligosyltrehalose synthase and maltooligosyltrehalose trehalohydrolase from Brevibacterium helvolum. Appl. Environ. Microbiol., 2000, 11: 4620-4624
Lee E.K., Seo S.B., Kim T.H., et al. Analysis of expressed sequence tags of Porphyra yezoensis. Mol. Cells, 2000, 10: 338-342
Leyman B. and Van Dijck, P. An unexpected plethora of trehalose synthesizing genes in Arabidopsis thaliana. Trends Plant Sci.2001,6: 5110-5113
Li G. and Quiros C.F. Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theor. Appl. Genet. 2001, 103: 455-461
Li G., Gao M., Yang B., et al. Gene for gene alignment between the Brassica and Arabidopsis genomes by direct transcriptome mapping. Theor. Appl. Genet. 2003, 107: 168-180
Liu Z.H., Anderson J.A., Hu J.G., et al. A wheat intervarietal genetic linkage map based on microsatellite and target region amplified polymorphism markers and its utility for detecting quantitative trait loci. Theor. Appl. Genet. 2005b, 111: 782–794
Ma J.H. and Cai S.Q. Cultivation and processing of Porphyra yezoensis[M]. Science Press. Beijing, 1996, 1-68
Mackenzie K. and Singh K. Water stress plating hypersensitivity of yeast: protective role of trehalose in Saccharomyces cerevisiae. J.Gen.Microbiol.1988, 134: 1661-1666
Maruta K., Hattori K., Nakada T., et al. Cloning and sequencing of trehalose biosynthesis genesfrom Arthrobacter sp. Q36. Biochim.Biophys.Acta., 1996a, 1289 (1): 10-13
Maruta K, Hattori K, Nakada T, et al. Cloning and sequencing of trehalose biosynthesis genes from Rhizobium sp.M-11. Biosci. Biotech. Biochem.,1996b, 60 (4): 717-720
McCouch S.R., Kochert G., Yu Z.H., et al. Molecular mapping of rice chromosomes. Theor. Appl. Genet. 1988, 76 (6): 815-829
Michelmore R.W., Paran I., Kesseli R.V., Identification of markers linked to disease-resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proc. Nation. Acad. Sci., 1991, 88 (21): 9828-9832
Mitman G.G., van der Meer J.P. Meiosis, blade development and sex determination in Porphyra purpurea (Phodophyta). J. Phycol. 1994, 30: 147-159
Mizukami Y, Okauchi M, Kito H, et a1. Discrimination of laver cultivars with RAPD markers. Fish Sci, 1996, 62 (4): 547-551
Muehlbauer G. J., Specht J. E., Thomas-Compton M. A., et al. Near-isogenic line- a potential resource in the integration of conventional and molecular marker linkage maps. Crop Science, 1988, 28: 729-735
Muller J. and Xie Z.. Trehalose and trehalase in root nodules of various legumes. Plant Physiol. 1994, 90: 86-92
Muller J. and Boller A. Effects of validamycin A, a potent trehalase inhibitor, and phytohormones on trehalose metabolism in roots and root nodules of soybean and cowpea. Planta, 1995, 197: 362-368
Muller J. and Wiemken A. Trehalose metabolism in sugar sensing and plant development. Plant Sci.1999, 147: 37-47
Muller J. and Aeschbacher R. Trehalose and trehalase in Arabidopsis. Plant Physiol. 2001, 125: 1086-1093
Murry H.G., and Thompson W. F. Rapid isolation of high molecular weight plant DNA. Nucl. Acids Res. 1980, 8: 4321-4325
Nakada T. and Maruta, K. Purification and properties of a novel enzyme, maltooligosyl trehalose synthase, from Arthrobacter sp. Q36. Biosci. Biotech. Biochem. 1995, 59: 2210-2214
Nikaido I., Asamizu E., Nakajima M., et al. Generation of 10,154 Expressed Sequence Tags from a leafy gametophyte of a marine red alga, Porphyra yezoensis. DNA Research, 2000, 7: 223-227
Niwa K, Kikuchi N, Iwabuchi M, et al.Morphological and AFLP variation of Porphyra yezoensisUeda form narawaensis miura (Bangiales, Rhodophyta).Phycological Research, 2004, 52 (2): 180-186
Noda H. Health benefits and nutritional properties of nori. J. Appl. Phycol.1993, 5:255-258
Oscar J.M., Goddijn Kees Van Dun. Trehalose Metabolism in Plants. Trends Plant Sci., 1999, 4 (8): 315-319
Otting G. Protein hydrtion in apueomns solution.Science.1991, 254: 974
Paul M. and Pellny T. Enhancing photosynthesis with sugar signals. Trends Plant Sci. 2001, 6: 197-200
Peakall R., Gilmore S., Keys W., et al. Cross-Species Amplification of Soybean (Glycine max) Simple Sequence Repeats (SSRs) Within the Genus and Other Legume Genera: Implications for the Transferability of SSRs in Plants. Mol. Biol. Evol.1998,15 (10): 1275-1287
Philips D. and Wilson D. Soluble carbohydrates in legumes and nodulates nonlegumes. J. Agric. Food Chem.1984, 32: 1289-1291
Reith M. and Munholland J. A high-resolution gene map of the chloroplast genome of the red alga Porphyra purpurea. Plant Cell, 1993, 5: 465-475
Romero C. and Belles J. Expression of the yeast trehalose-6-phosphate synthase gene in transgenic tobacco plants: pleiotropic phenotypes include drought tolerance. Planta, 1997, 201:293-297
Rungis D., Berube Y., Zhang J., et al. Robust simple sequence repeat markers for spruce (Picea spp) from expressed sequence tags. Theor. Appl. Genet. 2004, 109 (6): 1283-1294
Saito K., Yamazaki H., Ohnishi Y., et al. Production of trehalose synthase from abasidkycete. Grifola frondosa,in Eschherichia coil. App1. Microbiol. Biotechnol.1998, 50 (2): 193-198
Sheppard L.J., Franssen I. and Cape J.N. Frost hardiness of Norwayspruce treated with acid mist. Evaluation of the electrolyte leakage rate technique. Environ. Exp. Bot. 1995, 35: 139-149
Song S.H. and Zheng X.Y. Purity determination of Chinese cabbage F1 cultivars with RAPD markers. Acta Agriculturae Boreali-Sinica, 2000, 15 (4): 35-39
Stiller J.W. and Waaland J.R. Molecular analysis reveals cryptic diversity in Porphyra (Rhodophyta). J. Phycol, 1993, 29: 506-517
Sun J,W., Jin D.M., Zhou C.J., et al.Identification of Porphyra lines (Rhodophyta) by AFLP-DNA fingerprinting and molecular markers. Plant Molecular Biology Reporter 2005, 23 (3): 1-12
Sun J.W., Jin D. M., Weng M. L., et al. Development of SSR Primers from EST Sequences and Their Application in Germplasm Identification of Porphyra Lines. J. Europ. Phycol, 2006, 41: 329–336
Thaller M. and Schippa S. Conserved sequence motifs among bacterial, eukaryotic and archaeal phosphatases that define a new phosphohydrolase superfamily. Protein Sci. 1998, 7: 1647-1652
Thevelein J. and Hohmann S. Trehalose synthase: guard to the gate of glycolysis in yeast? Trends Biochem.Sci.1995, 20: 3-10
Tsusaki K., Nishimoto T., Nakada T., et al. Cloning and sequencing of trehalose synthase gene from Pimelobacter sp. R48. Biochim. Biophys. Acta, 1996, 1290 (1): 1-3
Tsusaki K., Nishimoto T., Nakada T, et al. Cloning and sequencing of trehalose synthase gene from Thermus aquaticus ATCC33923. Biochim.Biophys.Acta, 1997, 1334 (1): 28-32.
Van Vaeck C., Wera S., van Dijck P., et al. Analysis and modification of trehalose-6-phosphate levels in the yeast Saccharomyces cerevisiae with the use of Bacillus subtilis phosphotrehalase. Biochem J. 2001, 353: 157-162
Varshneya R.K., Sigmunda R., Bornera A. et al. Interspecific transferability and comparative mapping of barley EST-SSR markers in wheat, rye and rice. Plant Sci. 2005, 168: 195-202.
Vogel G. and Aeschbacher R. Trehalose-6-phosphate phosphatases from Arabidopsis thaliana: identification by functional complementation of the yeast tps2 mutant. Plant J. 1998, 13: 673-683
Vos P., Hogers R., Bleeker M, et a1. AFLP: a new technique for DNA fingerprinting. Nucl. Acids Res.1995, 23 (21): 4407-4414
Waaland J.R., Stiller J.W. and Cheney D.P. Macroalgal candidates for genomics. J. Phycol, 2004, 40 (1): 26-33
Wagner W. and Wiemken, A. Regulation of fructan metabolism in leaves of barley (Hordeum vulgare L. cv Gerbel). Plant Physiol.1986, 81: 444-447
Welsh J and McClelland M. Fingerprinting genomes using PCR with arbitrary primers. Nucl. Acids Res. 1990, 18 (24): 7213-7218
Wang B., Jia J.H., Shi J.F. et al. Identification of Porphyra lines using computerized DNA fingerprinting. Acta Oceanologica Sinica, 2001, 20 (3): 401-407
Wang X.L., Yang Y.X., Cong Y.Z. et al. DNA fingerprinting of selected Laminaria (Phaeophyta) gametophytes by RAPD markers. Aquaculture, 2004, 238: 143~153
Weng M.L., Liu B., Jin D.M. et al. Identification of 27 porphyra lines (Rhodophyta) by DNA fingerprinting and molecular markers. J. Appl. Phycol., 2005, 17 (1): 91-97
Wiemken A. Trehalose in yeast, stress protectant rather than reserve carbohydrate. J. Gen. Microbiol. 1990, 58: 209-217
Williams J.G., Kubelik A.R., Livak K.J., et a1. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.Nucl. Acids Res.1990, 18 (24): 6531-6535
Wingler A. and Fritzius T. Trehalose induced the ADP-glucose pyrophosphorylase gene, ApL3, and starch synthesis in Arabidopsis. Plant Physiol. 2000, 124: 105-114
Yarish C., Wilkes R., Chopin T., et al. Domestication of indigenous Porphyra species for commercial cultivation in Northeast America. World Aquaculture 1998, 29 (4): 26-55
Yeo E.T., Kwon H.B., Han S. E., et a1. Genetic engineering of drought resistant potato plants by introduction of the trehalose-6-phosphonte synthase (TPS1) gene from Saccharomyces cerevisiae. Mol.Cells,2000, 10 : 263-268
Zabeau M. and Vos P. Selective restriction fragment amplification:a general method for DNA fingerprinting [P]. European patient office publication 1993, 0535 858A1
Zhang C.L., Sun Z.L., Jin D.M., et al. Identification of maize inbred lines and validation of genetic relationship among maize inbred lines using RAPD marker. Maize Genetics Cooperation Newsletter, 1998, 72: 9-10
Zhang D., Zhang Z., Yang K., et al. Genetic mapping in (Populus tomentosa x Populus bolleana) and P. tomentosa Carr. using AFLP markers. Theor. Appl. Genet. 2004, 108 (4): 657-662
Zietkiewicz E., Rafalski A. and Labuda D. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics, 1994, 20: 176-183
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |