DNA适配体功能化三维纳米纤维及其对Hg~(2+)的吸附
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- 英文论文题名：DNA Aptamers Functionalized Three-Dimensional Nanofibers and the Adsorption of Hg~(2+)
- 论文作者：李延生 ; 张绍兴 ; 袁志鹏 ; 张淼 ; 焦翔宇 ; 温永强 ; 张学记
- 英文论文作者：Yansheng Li ; Shaoxing Zhang ; Zhipeng Yuan ; Miao Zhang ; Xiangyu Jiao ; Yongqiang Wen ; Xueji Zhang ; School of Chemistry and Bioengineering ; University of Science & Technology Beijing ; ENT ; Peking University Third Hospital
- 年：2016
- 作者机构：北京科技大学化学与生物工程学院;北京大学第三医院耳鼻喉科;
- 论文关键词：静电纺丝 ; 三维结构 ; 重金属 ; DNA适配体
- 英文论文关键词：electrospinning ; 3D structure ; heavy metal ; DNA aptamer
- 会议召开时间：2016-11-18
- 会议录名称：中国第四届静电纺丝大会（CICE 2016）摘要集
- 语种：中文
- 分类号：TQ340.64;TQ424
- 学会代码：ZGNM
- 会议名称：中国第四届静电纺丝大会(CICE 2016)
- 会议地点：中国北京
- 主办单位：中国复合材料学会超细纤维材料分会（筹）
- 学会名称：中关村科创纳米技术创新研究会
- 页数：1
- 文件大小：510k
- 原文格式：D
- 会议级别：全国
摘要
DNA适配体能与多种目标物质高特异性、高选择性地结合,因此被广泛应用于吸附净化领域。DNA适配体比蛋白质体积更小,经SELEX筛选富集后,可以拥有与抗原-抗体反应相匹敌的灵敏度,同时合成更容易,稳定性更好。本文制备了PAN-COOH与Si O_2纳米纤维膜,调整PAN与Si O_2的质量比,制得一种超轻且具有弹性的亲水性三维结构纳米纤维,然后对三维结构纳米纤维进行汞离子对应DNA适配体的修饰。然后将此具有亲水性的三维结构纳米纤维用于重金属离子的吸附实验,通过对比实验我们发现,修饰DNA适配体后的纤维对Hg~(2+)有着极强的吸附能力,其吸附率可达98.25%。
DNA aptamers can be combined with a variety of target substances with high specificity and high selectivity, so they are widely used in adsorption purification. DNA aptamers are smaller than proteins and can be amplified by SELEX screening. They are more sensitive to antigen-antibody reactions and are easier to synthesize and stabilize. In this paper, PAN-COOH and Si O2 nanofibrous membranes were prepared and a kind of ultra-light and elastic hydrophilic nanostructured nanofibers was prepared with the mass ratio of PAN to Si O2, and then the three-dimensional structure nanofibers were treated with DNA aptamers of mercury ions. The results show that three-dimensional structure nanofibers with modified DNA aptamers have strong adsorption capacity to Hg2 +, and the adsorption rate is up to 98.25%.
DNA aptamers can be combined with a variety of target substances with high specificity and high selectivity, so they are widely used in adsorption purification. DNA aptamers are smaller than proteins and can be amplified by SELEX screening. They are more sensitive to antigen-antibody reactions and are easier to synthesize and stabilize. In this paper, PAN-COOH and Si O2 nanofibrous membranes were prepared and a kind of ultra-light and elastic hydrophilic nanostructured nanofibers was prepared with the mass ratio of PAN to Si O2, and then the three-dimensional structure nanofibers were treated with DNA aptamers of mercury ions. The results show that three-dimensional structure nanofibers with modified DNA aptamers have strong adsorption capacity to Hg2 +, and the adsorption rate is up to 98.25%.
引文
[1]Li Y,Wen Y,et al.Nanomaterials 2016,6(24):2079-4991.
[2]Si Y,Yu J,Tang X,et al.Nature communications,2014,5:5802-5811.
[3]Li Y,Wen Y,et al.Sci Rep.2016,6:28411.
[4]Reneker D H,Yarin A L.Polymer,2008,49(10):2387-2425.
[5]Wang N,Si Y,Wang N,et al.Separation and Purification Technology,2014,126:44-51.
[2]Si Y,Yu J,Tang X,et al.Nature communications,2014,5:5802-5811.
[3]Li Y,Wen Y,et al.Sci Rep.2016,6:28411.
[4]Reneker D H,Yarin A L.Polymer,2008,49(10):2387-2425.
[5]Wang N,Si Y,Wang N,et al.Separation and Purification Technology,2014,126:44-51.