An alternative strategy for robust succinic acid production from food and industrial waste via fermentation of engineered Yarrowia lipolytica
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- 英文论文题名：An alternative strategy for robust succinic acid production from food and industrial waste via fermentation of engineered Yarrowia lipolytica
- 论文作者：杨晓锋 ; Chong Li ; Huaimin Wang ; Shi Gao ; 连思琪 ; Zhanglin Lin
- 英文论文作者：Xiaofeng Yang ; Chong Li ; Huaimin Wang ; Shi Gao ; Carol Sze Ki Lin ; Zhanglin Lin ; School of Bioscience and Bioengineering ; South China University of Technology ; School of Energy and Environment ; City University of Hong Kong
- 年：2017
- 作者机构：华南理工大学;School of Energy and Environment, City University of Hong Kong;香港城市大学;School of Bioscience and Bioengineering, South China University of Technology;
- 会议召开时间：2017-04-08
- 会议录名称：中国生物工程学会第二届青年科技论坛暨首届青年工作委员会学术年会论文集
- 语种：英文
- 分类号：TQ920.6
- 学会代码：IGSV
- 会议名称：中国生物工程学会第二届青年科技论坛暨首届青年工作委员会学术年会
- 会议地点：中国广东广州
- 主办单位：中国生物工程学会青年工作委员会
- 学会名称：中国生物工程学会
- 页数：1
- 文件大小：303k
- 原文格式：D
- 会议级别：全国
摘要
Succinic acid(SA) is an important platform chemical which has extensive application. Currently, SA production is mostly petrochemical-based. However, the bio-based SA market is predicted to reach at 710 kilotons by 2020[1]. To meet the increasing demand for bio-based SA production, the development of efficient and cost-effective process is urgently needed. Herein, we propose an alternative strategy for bio-based SA production from food and industrial waste streams by an engineered Yarrowia lipolytica. By fed-batch fermentation, up to 160 g/L SA was produced using crude glycerol as by-product from biodiesel industry by the engineered Y. lipolytica PGC01003. Subsequently, a novel in-situ fibrous bed bioreactor(isF BB) was developed to enhance the SA production, by which the productivity increased to 1.46 g/L/h. This SA production system has high operational stability in isF BB even after 460 hours fermentation with 12 repeated batches. By fed batch strategy, the resultant SA titer of 198.2 g/L was achieved. Future more, PGC01003 was adaptive evolved to restore the glucose metabolism. Then it was subsequently used in SA production using glucose-rich food waste hydrolysate. Our study promises yeast fermentation strategy for the highly efficient SA production from renewable source to promote the industrial upscaling. Additionally, it achieves the anticipated target of turning waste into value-added products.
Succinic acid(SA) is an important platform chemical which has extensive application. Currently, SA production is mostly petrochemical-based. However, the bio-based SA market is predicted to reach at 710 kilotons by 2020[1]. To meet the increasing demand for bio-based SA production, the development of efficient and cost-effective process is urgently needed. Herein, we propose an alternative strategy for bio-based SA production from food and industrial waste streams by an engineered Yarrowia lipolytica. By fed-batch fermentation, up to 160 g/L SA was produced using crude glycerol as by-product from biodiesel industry by the engineered Y. lipolytica PGC01003. Subsequently, a novel in-situ fibrous bed bioreactor(isF BB) was developed to enhance the SA production, by which the productivity increased to 1.46 g/L/h. This SA production system has high operational stability in isF BB even after 460 hours fermentation with 12 repeated batches. By fed batch strategy, the resultant SA titer of 198.2 g/L was achieved. Future more, PGC01003 was adaptive evolved to restore the glucose metabolism. Then it was subsequently used in SA production using glucose-rich food waste hydrolysate. Our study promises yeast fermentation strategy for the highly efficient SA production from renewable source to promote the industrial upscaling. Additionally, it achieves the anticipated target of turning waste into value-added products.
Succinic acid(SA) is an important platform chemical which has extensive application. Currently, SA production is mostly petrochemical-based. However, the bio-based SA market is predicted to reach at 710 kilotons by 2020[1]. To meet the increasing demand for bio-based SA production, the development of efficient and cost-effective process is urgently needed. Herein, we propose an alternative strategy for bio-based SA production from food and industrial waste streams by an engineered Yarrowia lipolytica. By fed-batch fermentation, up to 160 g/L SA was produced using crude glycerol as by-product from biodiesel industry by the engineered Y. lipolytica PGC01003. Subsequently, a novel in-situ fibrous bed bioreactor(isF BB) was developed to enhance the SA production, by which the productivity increased to 1.46 g/L/h. This SA production system has high operational stability in isF BB even after 460 hours fermentation with 12 repeated batches. By fed batch strategy, the resultant SA titer of 198.2 g/L was achieved. Future more, PGC01003 was adaptive evolved to restore the glucose metabolism. Then it was subsequently used in SA production using glucose-rich food waste hydrolysate. Our study promises yeast fermentation strategy for the highly efficient SA production from renewable source to promote the industrial upscaling. Additionally, it achieves the anticipated target of turning waste into value-added products.
引文
[1]Clark,S.2014.Global Bio Succinic Acid Market(Applications and Geography)-Size,Share,Trends,Analysis,Research,Future Demand,Scope and Forecast,2013-2020.