小苍兰芳樟醇合成酶基因的表达分析
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- 英文论文题名：Analysis of Linalool Synthase in Freesia
- 论文作者：樊荣辉 ; 黄敏玲 ; 钟淮钦 ; 罗远华
- 英文论文作者：Rong-Hui Fan ; Min-Ling Huang ; Huai-Qin Zhong ; Yuan-Hua Luo ; Institute of Crop Sciences ; Fujian Academy of Agricultural Science ; Flowers Research Center ; Fujian Academy of Agricultural Science ; Fujian Engineering Research Center for Characteristic Floriculture
- 年：2016
- 作者机构：福建省农业科学院作物研究所;福建省农业科学院花卉研究中心;福建省特色花卉工程技术研究中心;
- 论文关键词：小苍兰 ; 芳樟醇合成酶 ; 基因克隆 ; 表达分析
- 英文论文关键词：Freesia ; Linalool synthase ; gene cloning ; expression analysis
- 会议召开时间：2016-08-05
- 会议录名称：第四届全国花卉资源、育种、栽培及应用技术交流会论文汇编
- 语种：中文
- 分类号：Q943.2;S682.29
- 学会代码：EGYP
- 会议名称：第四届全国花卉资源、育种、栽培及应用技术交流会
- 会议地点：中国内蒙古呼和浩特
- 主办单位：中国园艺学会、中国园艺学会观赏园艺专业委员会
- 学会名称：中国园艺学会
- 页数：6
- 文件大小：1432k
- 原文格式：O
- 会议级别：全国
摘要
采用RT-PCR方法从小苍兰花瓣中克隆到1个LIS基因,含有完整的cDNA开放阅读框(ORF),共1779个碱基。与其他物种的芳樟醇合成酶氨基酸序列都有一定的同源性,达到40%-95%。应用荧光定量PCR分析表明,L/S在小苍兰品种White Wing-2中表达量最高,在花瓣中高表达,且在花发育的始花期表达量最高。
In the study,the full-length cDNA sequence of LIS gene was cloned from petals of freesia hybrida using RT-PCR.The cDNA included a whole open reading frame of 1779 bp.The amino acid was highly conserved compared with other LIS homologues and shared up to 40%-95%homology with other LIS.The fluorescent quantitative PCR analysis indicated that LIS was highly expressed in early flowering period,the transcript level was highest in White Wing-2 and petals.
In the study,the full-length cDNA sequence of LIS gene was cloned from petals of freesia hybrida using RT-PCR.The cDNA included a whole open reading frame of 1779 bp.The amino acid was highly conserved compared with other LIS homologues and shared up to 40%-95%homology with other LIS.The fluorescent quantitative PCR analysis indicated that LIS was highly expressed in early flowering period,the transcript level was highest in White Wing-2 and petals.
引文
[1]任雪冬,程光荣,王永明.顶空萃取-气相色谱-质谱法分析香雪兰的挥发性成分.质谱学报(Ren Xuedong,Cheng Guangrong,Wang Yongmin.Analysis of volatile flavor components in Freesia refacta by headspace extraction GC/MS.Journal of Chinese mass spectrometry society)2007;28(2):83-86.
[2]刘宝峰高丰展房强王丽.间接顶空固相微萃取-气相色谱-质谱法对红花香雪兰天然花香成分的分析.分析化学(Liu Baofeng,Gao Fengzhan,Fang Qiang,Wang Li.Determination of red Freesia flower volatiles with indirect headspace solid phase microextraction coupled to gas chromatography and mass spectrometry.Chinese Journal of Analytical Chemistry)2016,44(3):444-450.
[3]Knudsen ET,Tollsten L.Trends in floral scent chemistry in pollination syndromes:floral scent composition in mothpollinated taxa.Bot J Linn Soc 1993;113:263-284.
[4]Pare PW,Tumlinson JH.De novo biosynthesis of volatiles induced by insect herbivory in cotton plants.Plant Physiol1997;114:1161-1167.
[5]Shulaev V,Silverman P,Raskin I.Airborne signaling by methyl salicylate in plant pathogen resistance.Nature 1997;385:718-721.
[6]Croteau R,Kutchan TM,Lewis NG Natural products:Secondary metabolites/Biochemistry and Molecular Biology of Plants.ASPP 2000:1250-1318
[7]Yonekura Sakakibara K,Saito K.Functional genomics for plant natural product biosynthesis[J]Natural Product Reports,2009.26(11):1466-1487.
[8]樊荣辉,黄敏玲,钟淮钦,吴建设,叶秀仙.2011.花香的生物合成、调控及基因工程研究进展.中国细胞生物学学报(Fan Ronghui,Huang Minting,Zhong Huaiqin,Wu Jianshe,Ye Xiuxian.Adavances in biosynthesis,regulation and genetic engineering of floral scent.Chinese journal of cell biology)33(9):1028-1036.
[9]Yu FN,Utsumi R.Diversity,regulation.and genetic manipulation of plant mono and sesquiterpenoid biosynthesis[J].Cell Mol Life Sci.2009,66:3043-3052
[10]Fhnrich A,Krause K,Piechulla B.Product variability of the Cineole Cassette'monoterpene synthases of related Nicotiana species.[J].Mol Plant,2011.4:965-984
[11]Aubourg S,Lecharny A,Bohlmann J.Genomic analysis of the terpenoid synthase(AtTPS)gene family of Arabidopsis thaliana[J].MolGenet Genomics,2002,267(6):730-745
[12]Sacchettini JC,PouherCD.Creating isoprenoid diversity[J].Science,1997,277:1788-1789.
[13]Tholl D,Kish CM,Orlova I,Sherman D,Gershenzon J,Pichersky E,et al.Formation of monoterpenes in Antirrhinum majus and Clarkia breweri flowers involves heterodimeric geranyl diphosphate synthases.Plant Cell 2004;16:977-992.
[14]Boatright J,Negre F,Chen X,Kish CM,Wood B,Peel G,et al.Understanding in vivo benzenoid metabolism in petunia petal tissue.Plant Physiol 2004;135:1993-2011.
[15]Dudareva N,Pichersky E.Metabolic engineering of floral scent of ornamentals.J Crop Improvement 2006;18:325-346.
[16]Facchini P J,Chappell J.Gene family for anelicitor-induced sesquiter pene cyclase in tobacco.[J].Proe Natl Acad Sci USA,1992,89(22):11088—11092
[17]Degenhardt J,Kollner T G,Gershenzon J.Monoterpene and sesquiter pene synthases and the origin of terpene skeletal diversity in plants[J].Phytochemistry,2009.70(15-16):1621-1637.
[18]DudarevaN,Csekel,,BlancVM,etal.Evolution of floral scent in Clarkia:novel patterns of S-linalool synthase gene expression in the C.breweri flower[JJ].Plant Cell,1996,8:1137-1148.
[19]Dudareva N,Martin D,Kish C M,et al.(E)-13-Ocimene and myrcene synthase genes of floral scent biosynthesis in snapdragon:function and expression of three terpene synthase genes of a new TPS-subfamlly[J].Plant Cell,2003,1 5:1 227—1241
[20]Dudareva N,Cseke L,Blanc VM,Pichersky E.Evolution of Floral Scent in Clarkia:Novel Patterns of S-Linalool Synthase Gene Expression in the C.breweri Flower.The Plant Cell,8:1137-1 148,
[21]唐丽,唐芳,段经华,刘友全,钟秋平.金桂芳樟醇合成酶基因的克隆与序列分析.林业科学(Tang Li,Tang Fang,Duan Jinghua,Liu Youquan,Zhong Qiuping.Cloning and sequence analysis of a homologous linalool synthase gene involved in floral scents in Osmanthus fragrans var.thunbergii.Scientia silvae sinicae)2009,45(5):11-19.
[2]刘宝峰高丰展房强王丽.间接顶空固相微萃取-气相色谱-质谱法对红花香雪兰天然花香成分的分析.分析化学(Liu Baofeng,Gao Fengzhan,Fang Qiang,Wang Li.Determination of red Freesia flower volatiles with indirect headspace solid phase microextraction coupled to gas chromatography and mass spectrometry.Chinese Journal of Analytical Chemistry)2016,44(3):444-450.
[3]Knudsen ET,Tollsten L.Trends in floral scent chemistry in pollination syndromes:floral scent composition in mothpollinated taxa.Bot J Linn Soc 1993;113:263-284.
[4]Pare PW,Tumlinson JH.De novo biosynthesis of volatiles induced by insect herbivory in cotton plants.Plant Physiol1997;114:1161-1167.
[5]Shulaev V,Silverman P,Raskin I.Airborne signaling by methyl salicylate in plant pathogen resistance.Nature 1997;385:718-721.
[6]Croteau R,Kutchan TM,Lewis NG Natural products:Secondary metabolites/Biochemistry and Molecular Biology of Plants.ASPP 2000:1250-1318
[7]Yonekura Sakakibara K,Saito K.Functional genomics for plant natural product biosynthesis[J]Natural Product Reports,2009.26(11):1466-1487.
[8]樊荣辉,黄敏玲,钟淮钦,吴建设,叶秀仙.2011.花香的生物合成、调控及基因工程研究进展.中国细胞生物学学报(Fan Ronghui,Huang Minting,Zhong Huaiqin,Wu Jianshe,Ye Xiuxian.Adavances in biosynthesis,regulation and genetic engineering of floral scent.Chinese journal of cell biology)33(9):1028-1036.
[9]Yu FN,Utsumi R.Diversity,regulation.and genetic manipulation of plant mono and sesquiterpenoid biosynthesis[J].Cell Mol Life Sci.2009,66:3043-3052
[10]Fhnrich A,Krause K,Piechulla B.Product variability of the Cineole Cassette'monoterpene synthases of related Nicotiana species.[J].Mol Plant,2011.4:965-984
[11]Aubourg S,Lecharny A,Bohlmann J.Genomic analysis of the terpenoid synthase(AtTPS)gene family of Arabidopsis thaliana[J].MolGenet Genomics,2002,267(6):730-745
[12]Sacchettini JC,PouherCD.Creating isoprenoid diversity[J].Science,1997,277:1788-1789.
[13]Tholl D,Kish CM,Orlova I,Sherman D,Gershenzon J,Pichersky E,et al.Formation of monoterpenes in Antirrhinum majus and Clarkia breweri flowers involves heterodimeric geranyl diphosphate synthases.Plant Cell 2004;16:977-992.
[14]Boatright J,Negre F,Chen X,Kish CM,Wood B,Peel G,et al.Understanding in vivo benzenoid metabolism in petunia petal tissue.Plant Physiol 2004;135:1993-2011.
[15]Dudareva N,Pichersky E.Metabolic engineering of floral scent of ornamentals.J Crop Improvement 2006;18:325-346.
[16]Facchini P J,Chappell J.Gene family for anelicitor-induced sesquiter pene cyclase in tobacco.[J].Proe Natl Acad Sci USA,1992,89(22):11088—11092
[17]Degenhardt J,Kollner T G,Gershenzon J.Monoterpene and sesquiter pene synthases and the origin of terpene skeletal diversity in plants[J].Phytochemistry,2009.70(15-16):1621-1637.
[18]DudarevaN,Csekel,,BlancVM,etal.Evolution of floral scent in Clarkia:novel patterns of S-linalool synthase gene expression in the C.breweri flower[JJ].Plant Cell,1996,8:1137-1148.
[19]Dudareva N,Martin D,Kish C M,et al.(E)-13-Ocimene and myrcene synthase genes of floral scent biosynthesis in snapdragon:function and expression of three terpene synthase genes of a new TPS-subfamlly[J].Plant Cell,2003,1 5:1 227—1241
[20]Dudareva N,Cseke L,Blanc VM,Pichersky E.Evolution of Floral Scent in Clarkia:Novel Patterns of S-Linalool Synthase Gene Expression in the C.breweri Flower.The Plant Cell,8:1137-1 148,
[21]唐丽,唐芳,段经华,刘友全,钟秋平.金桂芳樟醇合成酶基因的克隆与序列分析.林业科学(Tang Li,Tang Fang,Duan Jinghua,Liu Youquan,Zhong Qiuping.Cloning and sequence analysis of a homologous linalool synthase gene involved in floral scents in Osmanthus fragrans var.thunbergii.Scientia silvae sinicae)2009,45(5):11-19.
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