致急性肝胰腺坏死病副溶血弧菌(VP_(AHPND))感染凡纳滨对虾后的动态变化以及定量分析
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- 英文论文题名：The dynamic changes and quantitative analysis of Vibrio parahaemolyticus(VP_(AHPND)) in infected Peneaus vannamei
- 论文作者：陈蒙蒙 ; 董宣 ; 邱亮 ; 万晓媛 ; 黄倢
- 英文论文作者：CHEN Mengmeng ; DONG Xuan ; QIU Liang ; WANG Xiaoyuan ; HUANG Jie ; Yellow Sea Fisheries Research Institute ; Chinese Academy of Fishery Sciences
- 年：2016
- 作者机构：农业部海洋渔业可持续发展重点实验室黄海水产研究所中国水产科学研究院;上海海洋大学水产与生命技术学院;
- 论文关键词：致急性肝胰腺坏死病副溶血弧菌 ; 浸泡感染 ; 凡纳滨对虾 ; qPCR ; 组织分布
- 英文论文关键词：Vibrio parahaemolyticus ; immersing infection ; Peneaus vannamei ; qPCR ; tissue distribution
- 会议召开时间：2016-11-02
- 会议录名称：2016年中国水产学会学术年会论文摘要集
- 语种：中文
- 分类号：S945.49
- 学会代码：OGSB
- 会议名称：2016年中国水产学会学术年会
- 会议地点：中国四川成都
- 主办单位：中国水产学会、四川省水产学会
- 学会名称：中国水产学会
- 页数：1
- 文件大小：563k
- 原文格式：D
- 会议级别：全国
摘要
致急性肝胰腺坏死病副溶血弧菌(Vibrio parahaemolyticus,VP_(AHPND))是对虾急性肝胰腺坏死病(Acutehepatopancreatic necrosis disease,AHPND)主要病原菌。本研究利用VP_(AHPND)(V-1)对凡纳滨对虾进行浸泡感染试验,探究浸泡感染后VP_(AHPND)在各组织中数量的动态变化。实验设立实验组和对照组,用菌株V-1对实验组浸泡感染,浓度为10^5/mL水体,试验组不进行感染处理,感染后分别于2d-11d采集各组对虾肝胰腺、鳃、肠道、肌肉,实时荧光定量PCR方法检测各组织中VP_(AHPND)数量。实验结果显示,在各取样时间段实验组凡纳滨对虾各组织在均能检测到VP_(AHPND),对照组未检测到VP_(AHPND);实验组对虾肝胰腺组织病理切片显示出VP_(AHPND)的典型症状;最高菌量出现在肝胰腺中,其次是肠道、肌肉、鳃;各组织中VP_(AHPND)数量大致分布在10^1-10^4 copies/mg组织;组织内的菌量随时间大体呈现先上升后下降的趋势。
Vibrio parahaemolyticus(VPAHPND) is the major pathogen is acute hepatopancreatic necrosis disease(AHPND). In this study,Peneaus vannamei were exposed to VPAHPND V-1 by immersion, to explore the dynamic changes of V-1 number in the tissues after immersing infection.The experiment included the experimental group and control group, only to infect the experimental group with the VPAHPND V-1 by immersion at a concentration of 10^5/mL water,no treatment to control group.To collect shrimp hepatopancreas, gill, intestinal and muscle respectively at 2d-11 d after infection, detecting the quantity by qPCR. The experimental results show that the experimental group can detect V-1 in the tissues at all sampling point, not detected in control group; histopathological biopsy showed the shrimp hepatopancreas of experimental group had typical symptoms.
Vibrio parahaemolyticus(VPAHPND) is the major pathogen is acute hepatopancreatic necrosis disease(AHPND). In this study,Peneaus vannamei were exposed to VPAHPND V-1 by immersion, to explore the dynamic changes of V-1 number in the tissues after immersing infection.The experiment included the experimental group and control group, only to infect the experimental group with the VPAHPND V-1 by immersion at a concentration of 10^5/mL water,no treatment to control group.To collect shrimp hepatopancreas, gill, intestinal and muscle respectively at 2d-11 d after infection, detecting the quantity by qPCR. The experimental results show that the experimental group can detect V-1 in the tissues at all sampling point, not detected in control group; histopathological biopsy showed the shrimp hepatopancreas of experimental group had typical symptoms.
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