UPLC-MS/MS法测定鸡肉组织中金刚烷胺残留
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- 英文论文题名：Determination of Amantadine Residues in Chicken Tissue by UPLC-MS/MS
- 论文作者：魏秀丽 ; 高迎春 ; 陈玲 ; 陆庆泉 ; 张呈军
- 英文论文作者：WEI Xiu-li ; GAO Yiny-chun ; CHEN Ling ; LU Qing-quan ; ZHANG Cheng-jun ; Institute of Veterinary Drug Quality Inspection of Shandong Province
- 年：2016
- 作者机构：山东省兽药质量检验所;
- 论文关键词：金刚烷胺 ; 鸡肉组织 ; 残留 ; 超高效液相-串联质谱法
- 英文论文关键词：amantadine ; chicken tissue ; residues ; UPLC-MS/MS
- 会议召开时间：2016-09-18
- 会议录名称：中国畜牧兽医学会动物药品学分会第五届全国会员代表大会暨2016年学术年会论文集
- 语种：中文
- 分类号：S859.84;O657.63
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物药品学分会第五届全国会员代表大会暨2016年学术年会
- 会议地点：中国四川成都
- 主办单位：中国畜牧兽医学会动物药品学分会
- 学会名称：中国畜牧兽医学会
- 页数：5
- 文件大小：422k
- 原文格式：O
- 会议级别：全国
摘要
建立了鸡肉组织中金刚烷胺残留检测的UPLC-MS/MS方法。采用三氯乙酸:乙腈(1:1,V/V)提取试样中残留的金刚烷胺,SPE净化浓缩,ACQUITY UPLCTMBEH C_(18)色谱柱为分离柱,正离子扫描上机测定。结果显示,金刚烷胺在1~20 ng/mL的范围内线性关系良好(R~2=0.997);样品检出限为1 ng/g,定量限为2 ng/g。在2、5、10 ng/g添加水平的回收率为85%~125%,批内批间变异系数均小于20%。本方法快速、灵敏、重现性好,适用于鸡肉中金刚烷胺的残留检测。
A mothod was established for determination of amantadine residues in chicken tissue by UPLC-MS/MS.Samples were extracted by acetocaustin- acetonitrile(1:1,V/V),Sample purification and concentration were performed by SPE with watersoasis mcx column.Then the reconstituted sample solution was analyzed by UPLC-MS/MS under positive ion mode,with ACQUITY UPLCTMBEH C_(18) as the analytical column.The results showed that the good linear range was 1.0~20 ng/mL(R~2=0.997).The detection limit was 1 ng/g,and the quantitation limit was 2 ng/g.The recoveries ranged from 85%~125%and the relative standard deviations were below 20%following analysis of spiked at concentrations 2,5,10 ng/g.The mothod was reliable,sensitive and reproducibility,adapts to the determination of amantadine residues in chiken tissue.
A mothod was established for determination of amantadine residues in chicken tissue by UPLC-MS/MS.Samples were extracted by acetocaustin- acetonitrile(1:1,V/V),Sample purification and concentration were performed by SPE with watersoasis mcx column.Then the reconstituted sample solution was analyzed by UPLC-MS/MS under positive ion mode,with ACQUITY UPLCTMBEH C_(18) as the analytical column.The results showed that the good linear range was 1.0~20 ng/mL(R~2=0.997).The detection limit was 1 ng/g,and the quantitation limit was 2 ng/g.The recoveries ranged from 85%~125%and the relative standard deviations were below 20%following analysis of spiked at concentrations 2,5,10 ng/g.The mothod was reliable,sensitive and reproducibility,adapts to the determination of amantadine residues in chiken tissue.
引文
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[3].云环,张朝晖,罗生亮,等.固相萃取/LC-MS-MS法检测动物源性食品中的金刚烷胺[J].现代仪器,2009,06:42-45.
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[5].农业部文件农医发[2005]33号关于清查金刚烷胺等抗病毒药物的紧急通知[Z].2005.
[2].李云巍,周小淳,秦焕杰.同时测定人血浆中金刚烷胺、马来酸氯苯那敏的分析方法[J].中国医药指南,2012,10(26):450-452.
[3].云环,张朝晖,罗生亮,等.固相萃取/LC-MS-MS法检测动物源性食品中的金刚烷胺[J].现代仪器,2009,06:42-45.
[4].中华人民共和国农业部公告第560号[Z].2005.
[5].农业部文件农医发[2005]33号关于清查金刚烷胺等抗病毒药物的紧急通知[Z].2005.
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