Prevalence and RT/RNAse H Genealogy of Isolates of Sugarcane bacilliform viruses from China
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- 英文论文题名：Prevalence and RT/RNAse H Genealogy of Isolates of Sugarcane bacilliform viruses from China
- 论文作者：WU Xiao-bin ; ALABI Olufemi J ; DAMAJ Mona B ; SUN Sheng-ren ; MIRKOV T.Erik ; FU Hua-ying ; CHEN Ru-kain ; GAO San-ji
- 英文论文作者：WU Xiao-bin ; ALABI Olufemi J ; DAMAJ Mona B ; SUN Sheng-ren ; MIRKOV T.Erik ; FU Hua-ying ; CHEN Ru-kain ; GAO San-ji ; Key Laboratory of Sugarcane Biology and Genetic Breeding ; Ministry of Agriculture ; Fujian Agriculture and Forestry University ; Department of Plant Pathology and Microbiology ; Texas A&M AgriLife Research and Extension Center ; Guangxi Collaborative Innovation Center of Sugarcane Industry ; Guangxi University
- 年：2016
- 作者机构：Key Laboratory of Sugarcane Biology and Genetic Breeding,Ministry of Agriculture,Fujian Agriculture and Forestry University;Department of Plant Pathology and Microbiology,Texas A&M AgriLife Research and Extension Center;Guangxi Collaborative Innovation Center of Sugarcane Industry,Guangxi University;
- 会议召开时间：2016-08-05
- 会议录名称：中国植物病理学会2016年学术年会论文集
- 英文会议录名称：Proceedings of the Annual Meeting of Chinese Society for Plant Pathology(2016)
- 语种：英文
- 分类号：S435.661
- 学会代码：ZGVS
- 会议名称：中国植物病理学会2016年学术年会
- 会议地点：中国江苏南京
- 主办单位：中国植物病理学会
- 学会名称：中国植物病理学会
- 页数：1
- 文件大小：54k
- 原文格式：O
- 会议级别：全国
摘要
Sugarcane bacilliform viruses(SCBV;genus Badnavirus;family Caulimoviridae) are a threat to the global exchange of sugarcane germplasm.A total of 280 sugarcane- leaf tissue samples collected from six provinces in China,25 from three states in the USA and five from Queensland,Australia were tested for the presence of SCBV by polymerase chain reaction(PCR) using newly designed degenerate primers targeting a 720 base pair(bp) fragment of the reverse transcriptase/ribonuclease H(RT/RNAse H) genomic region.PCR amplified fragments from 94 SCBV-positive samples were then cloned,sequenced and analyzed for their genetic diversity.The results revealed considerable haplotype diversity within individual SCBV isolates.Recombination analyses showed weak signatures of recombination among some of the SCBV sequences.Phylogenetic analysis revealed the segregation of global SCBV isolates into three major monophyletic clades encompassing 18 subgroups,including five previously undescribed subgroups named as SCBV-N to-R.Population genetic analysis data indicated that relatively low levels of genetic exchange have occurred between SCBV populations from different sugarcane-producing regions of the world.Together with the new set of degenerate SCBV-specific primers designed in this study,our results will advance the understanding of SCBV population structure in a semi-perennial host plant and aid the screening of global sugarcane germplasm to minimize the spread of genetic variants of the virus via contaminated plant materials.
Sugarcane bacilliform viruses(SCBV;genus Badnavirus;family Caulimoviridae) are a threat to the global exchange of sugarcane germplasm.A total of 280 sugarcane- leaf tissue samples collected from six provinces in China,25 from three states in the USA and five from Queensland,Australia were tested for the presence of SCBV by polymerase chain reaction(PCR) using newly designed degenerate primers targeting a 720 base pair(bp) fragment of the reverse transcriptase/ribonuclease H(RT/RNAse H) genomic region.PCR amplified fragments from 94 SCBV-positive samples were then cloned,sequenced and analyzed for their genetic diversity.The results revealed considerable haplotype diversity within individual SCBV isolates.Recombination analyses showed weak signatures of recombination among some of the SCBV sequences.Phylogenetic analysis revealed the segregation of global SCBV isolates into three major monophyletic clades encompassing 18 subgroups,including five previously undescribed subgroups named as SCBV-N to-R.Population genetic analysis data indicated that relatively low levels of genetic exchange have occurred between SCBV populations from different sugarcane-producing regions of the world.Together with the new set of degenerate SCBV-specific primers designed in this study,our results will advance the understanding of SCBV population structure in a semi-perennial host plant and aid the screening of global sugarcane germplasm to minimize the spread of genetic variants of the virus via contaminated plant materials.
Sugarcane bacilliform viruses(SCBV;genus Badnavirus;family Caulimoviridae) are a threat to the global exchange of sugarcane germplasm.A total of 280 sugarcane- leaf tissue samples collected from six provinces in China,25 from three states in the USA and five from Queensland,Australia were tested for the presence of SCBV by polymerase chain reaction(PCR) using newly designed degenerate primers targeting a 720 base pair(bp) fragment of the reverse transcriptase/ribonuclease H(RT/RNAse H) genomic region.PCR amplified fragments from 94 SCBV-positive samples were then cloned,sequenced and analyzed for their genetic diversity.The results revealed considerable haplotype diversity within individual SCBV isolates.Recombination analyses showed weak signatures of recombination among some of the SCBV sequences.Phylogenetic analysis revealed the segregation of global SCBV isolates into three major monophyletic clades encompassing 18 subgroups,including five previously undescribed subgroups named as SCBV-N to-R.Population genetic analysis data indicated that relatively low levels of genetic exchange have occurred between SCBV populations from different sugarcane-producing regions of the world.Together with the new set of degenerate SCBV-specific primers designed in this study,our results will advance the understanding of SCBV population structure in a semi-perennial host plant and aid the screening of global sugarcane germplasm to minimize the spread of genetic variants of the virus via contaminated plant materials.
引文