月季杂交幼胚培养技术研究

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• 英文论文题名：Protocols of Immature Embryo Culture for R.chinensis ‘Narrow-leaflet Climber’ × R.chinensis ‘Old Blush’
• 论文作者：徐庭亮 ; 廖雪芹 ; 张逸璇 ; 杜明杰 ; 谭炯锐 ; 潘会堂 ; 张启翔
• 英文论文作者：XU Ting-liang ; LIAO Xue-qin ; ZHANG Yi-xuan ; DU Ming-jie ; TAN Jiong-rui ; PAN Hui-tang ; ZHANG Qi-xiang ; Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding ; National Engineering Research Center for Floriculture ; Beijing Laboratory of Urban and Rural Ecological Environment and College of Landscape Architecture ; Beijing Forestry University
• 年：2016
• 作者机构：花卉种质创新与分子育种北京市重点实验室国家花卉工程技术研究中心城乡生态环境北京实验室园林学院北京林业大学;
• 论文关键词：月季 ; 杂交 ; 胚培养 ; 胚龄
• 英文论文关键词：R.chinensis Hybridization ; Immature embryo culture ; Embryo age
• 会议召开时间：2016-07-19
• 会议录名称：中国观赏园艺研究进展2016
• 语种：中文
• 分类号：S685.12
• 学会代码：EGYP
• 会议名称：2016年中国观赏园艺学术研讨会
• 会议地点：中国湖南长沙
• 主办单位：中国园艺学会观赏园艺专业委员会、国家花卉工程技术研究中心
• 学会名称：中国园艺学会
• 页数：6
• 文件大小：1130k
• 原文格式：O
• 会议级别：全国

摘要

为了有效缩短蔷薇属植物的杂交育种周期,本试验以窄叶藤本月月红(R.chinensis‘Narrow-leaflet Climber')为母本,月月粉(R.chinensis‘Old Blush')为父本进行人工杂交,研究了早期杂交胚培养的技术程序,分析了胚龄对胚培养效果的影响,比较了幼胚培养和传统的杂交种子育苗方式获得杂交后代苗的效率。结果表明,该杂交组合适宜幼胚培养的最早胚龄为授粉后90天左右,采用幼胚培养方式,从杂交授粉到获得杂种后代的时间较常规方式缩短了109天(31.48%),SSR分子标记分析证明胚培养获得的后代为真杂种。在此基础上,建立了月季花品种间杂交幼胚培养的技术程序。
To shorten the period of cross breeding in genus Rosa,in our experiment,immature embryos of R.chinensis' Narrow-leaflet Climber' × R.chinensis' Old Blush' were in vitro cultivated,and the effects of embryo age on seed germination was analyzed.The results showed that the seeds of more than 90 days old germinated successfully with gemination rate 16.67—23.53%.The seedlings were identified to be true hybrids with SSR mrkers analysis.Compared to traditional hybridization procedure,the breeding period decreased by 31.4%(109 d) with 90-day-old embryos culture method.Comprehensive analysis of different factors,immature embryo culture was considered an ideal way to improve hybridization breeding efficency.

引文

1.AbdolmohammdiM.,KermaniM.J.,ZakizadehH.,Y.Hamidoghli.In vitro embyro germination and interploidy hybridization of rose(Rosa sp.)[J].Euphytica,2014,198:255-264.
    2.Aleza P.&Juarez J.&Hernandez M.&OUitrault P.&NavarroL.Implementation of extensivecitrus triploid breeding programs based on 4x x 2x sexual hybridisations[J].Tree Genetics&Genomes,2012,8:1293-1306.
    3.Canli F.A.,Kazaz S.Biotechnology of roses;progress and future prospects[J].2009,ISSN 1302-7085:167-187.
    4.Krishna H.,Singh S.K..Biotechnological advances in mango(Mangifera indica L)and their future implication in crop improvement[J].Biotechnol Adv.,2007,25(3);2237,25.
    5.LloydD.,RobertsA.V.&ShortK.C..The induction In Vitro of adventitions shoots in Rosa[J].Euphytica,1988,37:31-36.
    6.PommerC.V.,RammingD.W.,EmershadR.L.Influence of grape genotype,ripening season,seed trace size,and culture date on in ovule embryo development and plant formation[J].Bragantia,1995,54(2):237-249.
    7.Shen X.,Gmitter F.G.,Grosser J.W.,Immature embryo rescue and culture[J].Methods Mol Biol.,2011,710:75-92.
    8.Gudin S.,&AreneL..Influence of endocarp thickness on rose achene germination;genetic and environmental factors[J].HortScience.1980,25(7):786-788.
    9.Yu C.,Luo L,Pan H.T.,Guo X.L,WanH.H.and Zhang Q.X..Filling gaps with construction of a genetic linkage map in tetraploid roses[J].Frontiers in Plant Science.2015,5:796.
    10.鲍平秋,丁艳丽,张雷.月季杂交育种研究[J].中国园林,2009(8):97-99.
    11.陈俊愉.中国十大名花[M].上海:上海文化出版社,1989.
    12.祁业凤,刘孟军,王玖瑞,等.果树胚培养研究进展[J].生物技术,2002,12(3):46-48.
    13.韩进,丰震,赵兰勇,等.月季杂交育种的初步研究[J].山东农业大学学报(自然科学版),2004,35(4).
    14.刘艳梅.潘会堂,张启翔.小报春与欧报春杂交育种初步研究[J].中国观赏园艺研究进展2014:110-120.
    15.梁春莉,赵锦,刘孟军.冬枣极早期幼胚培养成苗技术研究[J].园艺学报,2014,41(10):21154.
    16.马燕,陈俊愉.培育刺玫月季新品种的初步研究(Ⅳ)[J].北京林业大学学报,1992,13(2):103-109.
    17.石文芳,郝瑞杰,王史琴,等.4个梅花品种的胚培养及愈伤组织诱导研究[J].中国农学通报,2012,28(10):198-201.
    18.孙宪芝.北林月季杂交育种技术体系初探[D].北京林业大学,2004.
    19.谢小波,求盈盈,郑锡良,等.杨梅种间杂交及杂种F,的胚培养[J].果树学报,2009,26(4):507-510.
    20.赵红霞.蔷薇属植物与现代月季品种杂交及主要性状与SSR标记的连锁分析[D].北京林业大学,2015.
    21.张佐双,朱秀珍.中国月季[M].北京:中国林业出版社,2006.517-520.