Delineating topographic and circuital organization of primary visual cortex thalamus projecting neurons by rabies virus tracing system
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- 英文论文题名：Delineating topographic and circuital organization of primary visual cortex thalamus projecting neurons by rabies virus tracing system
- 论文作者：Liang Li ; Yajie Tang ; Jinsong Yu ; Jinxia Dai ; Leqiang Sun ; Zhenfang Fu ; Gang Cao
- 英文论文作者：Liang Li ; Yajie Tang ; Jinsong Yu ; Jinxia Dai ; Leqiang Sun ; Zhenfang Fu ; Gang Cao ; State Key Laboratory of Agricultural Microbiology ; Huazhong Agricultural University ; College of Veterinary Medicine ; Huazhong Agricultural University ; Key Laboratory of Development of Veterinary Diagnostic Products ; Ministry of Agriculture ; College of Veterinary Medicine ; Huazhong Agricultural University
- 年：2016
- 作者机构：State Key Laboratory of Agricultural Microbiology,Huazhong Agricultural University;College of Veterinary Medicine,Huazhong Agricultural University;Key Laboratory of Development of Veterinary Diagnostic Products,Ministry of Agriculture,College of Veterinary Medicine,Huazhong Agricultural University;
- 会议召开时间：2016-08-20
- 会议录名称：中国畜牧兽医学会动物解剖及组织胚胎学分会第十九次学术研讨会论文集
- 语种：英文
- 分类号：S852.65;Q42
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物解剖及组织胚胎学分会第十九次学术研讨会
- 会议地点：中国河南郑州
- 主办单位：中国畜牧兽医学会动物解剖及组织胚胎学分会
- 学会名称：中国畜牧兽医学会
- 页数：2
- 文件大小：119k
- 原文格式：O
- 会议级别：全国
摘要
Visual system integrates light from the colorful world and precisely decodes them into information in our brain to process vision perception and trigger subsequent vision-evoked effects including escape from danger,tendency to interest or generation of memory.The whole process is vitally relied on a sophisticated and elegant visual circuit,among which the primary visual(V1)cortex plays a central role.Visual circuit from retina to V1 cortex is highly organized into a well-designed topographic structure.In most higher animal,V1 cortex is designed as cortical column including ocular dominance column and orientation column to perform precise visual information processing in human,primates and cats.Even for mice,of which the visual system is less developed,V1 cortex is divided into binocular and monocular area.V1 cortex mainly receives thalamic inputs from lateral geniculate nucleus(LGN),a key relay center for visual information.Reciprocally,it sends a strong feedback output to dorsal LGN(dLGN).Output projections from V1 cortex were also observed to directly target on cortical structure including lateral part of secondary visual cortex(V2L),contralateral visual cortex,and subcortical structure including superior colliculus(SCs),lateral posterior nucleus of thalamus(LP) as well as lateral dorsal nucleus of thalamus(LD).V1 cortex was architecturally divided into six layers from layer I to layer V1.Layer V and layer V1 neurons are the main output part to other cortical and subcortical brain regions.It is more noteworthy that neurons originated from same cell type in same layer of V1 will show different morphology and express distinct protein In mouse V1,pyramidal neurons of layer V are subdivided into five types based on different morphology,of which all type I neurons project to superior colliculus,suggesting a micro-level module in visual circuit.Due to the lack of subnucleus level fine mapping system,it still remains unclear that how exactly the V1 cortex neurons are topographically distributed and elegantly organized to target different brain regions to participate specific and precise vision related function.The fine mapping of V1 cortex circuit architecture will be essential for addressing the exact function of visual circuit in vision evoked behavior.Here we constructed a set of recombinant rabies virus expressing different florescent proteins or GCaMP,and applied them to systematically delineate the architecture V1 cortex.By simultaneously retrograde labeling different thalamic nucleus projecting V1 neurons with different florescent rabies virus,we reconstructed topographic pattern of V1 subnucleus.Their corresponding upstream circuits were further explored by mono-trans-synaptic tracing.Notably,significant alterations of V1 projection to thalamic nucleus were observed in monocular deprivation model.Furthermore,we analyzed spontaneous dendritic activity of different subset of V1 neurons projecting to different thalamic nuclei by in vivo calcium imaging and demonstrated the topographic and functional organization of V1 cortex subnucleus.Taken together,our work systematically investigated the topographic pattern,functional organization and plasticity of Vl-thalamus projection and their different upstream circuitry.These results could pave the way to explore the detailed function and computation of Vl-thalamus circuit and understand visual dependent behavior.Meanwhile,our multi-floresences rabies virus retrograde and trans-synaptic tracing strategy might be extensively applied to delineate the detailed architecture of any regime in nervous system and shed a light on the fine map of our brain in sub-nucleus level.
Visual system integrates light from the colorful world and precisely decodes them into information in our brain to process vision perception and trigger subsequent vision-evoked effects including escape from danger,tendency to interest or generation of memory.The whole process is vitally relied on a sophisticated and elegant visual circuit,among which the primary visual(V1)cortex plays a central role.Visual circuit from retina to V1 cortex is highly organized into a well-designed topographic structure.In most higher animal,V1 cortex is designed as cortical column including ocular dominance column and orientation column to perform precise visual information processing in human,primates and cats.Even for mice,of which the visual system is less developed,V1 cortex is divided into binocular and monocular area.V1 cortex mainly receives thalamic inputs from lateral geniculate nucleus(LGN),a key relay center for visual information.Reciprocally,it sends a strong feedback output to dorsal LGN(dLGN).Output projections from V1 cortex were also observed to directly target on cortical structure including lateral part of secondary visual cortex(V2L),contralateral visual cortex,and subcortical structure including superior colliculus(SCs),lateral posterior nucleus of thalamus(LP) as well as lateral dorsal nucleus of thalamus(LD).V1 cortex was architecturally divided into six layers from layer I to layer V1.Layer V and layer V1 neurons are the main output part to other cortical and subcortical brain regions.It is more noteworthy that neurons originated from same cell type in same layer of V1 will show different morphology and express distinct protein In mouse V1,pyramidal neurons of layer V are subdivided into five types based on different morphology,of which all type I neurons project to superior colliculus,suggesting a micro-level module in visual circuit.Due to the lack of subnucleus level fine mapping system,it still remains unclear that how exactly the V1 cortex neurons are topographically distributed and elegantly organized to target different brain regions to participate specific and precise vision related function.The fine mapping of V1 cortex circuit architecture will be essential for addressing the exact function of visual circuit in vision evoked behavior.Here we constructed a set of recombinant rabies virus expressing different florescent proteins or GCaMP,and applied them to systematically delineate the architecture V1 cortex.By simultaneously retrograde labeling different thalamic nucleus projecting V1 neurons with different florescent rabies virus,we reconstructed topographic pattern of V1 subnucleus.Their corresponding upstream circuits were further explored by mono-trans-synaptic tracing.Notably,significant alterations of V1 projection to thalamic nucleus were observed in monocular deprivation model.Furthermore,we analyzed spontaneous dendritic activity of different subset of V1 neurons projecting to different thalamic nuclei by in vivo calcium imaging and demonstrated the topographic and functional organization of V1 cortex subnucleus.Taken together,our work systematically investigated the topographic pattern,functional organization and plasticity of Vl-thalamus projection and their different upstream circuitry.These results could pave the way to explore the detailed function and computation of Vl-thalamus circuit and understand visual dependent behavior.Meanwhile,our multi-floresences rabies virus retrograde and trans-synaptic tracing strategy might be extensively applied to delineate the detailed architecture of any regime in nervous system and shed a light on the fine map of our brain in sub-nucleus level.
Visual system integrates light from the colorful world and precisely decodes them into information in our brain to process vision perception and trigger subsequent vision-evoked effects including escape from danger,tendency to interest or generation of memory.The whole process is vitally relied on a sophisticated and elegant visual circuit,among which the primary visual(V1)cortex plays a central role.Visual circuit from retina to V1 cortex is highly organized into a well-designed topographic structure.In most higher animal,V1 cortex is designed as cortical column including ocular dominance column and orientation column to perform precise visual information processing in human,primates and cats.Even for mice,of which the visual system is less developed,V1 cortex is divided into binocular and monocular area.V1 cortex mainly receives thalamic inputs from lateral geniculate nucleus(LGN),a key relay center for visual information.Reciprocally,it sends a strong feedback output to dorsal LGN(dLGN).Output projections from V1 cortex were also observed to directly target on cortical structure including lateral part of secondary visual cortex(V2L),contralateral visual cortex,and subcortical structure including superior colliculus(SCs),lateral posterior nucleus of thalamus(LP) as well as lateral dorsal nucleus of thalamus(LD).V1 cortex was architecturally divided into six layers from layer I to layer V1.Layer V and layer V1 neurons are the main output part to other cortical and subcortical brain regions.It is more noteworthy that neurons originated from same cell type in same layer of V1 will show different morphology and express distinct protein In mouse V1,pyramidal neurons of layer V are subdivided into five types based on different morphology,of which all type I neurons project to superior colliculus,suggesting a micro-level module in visual circuit.Due to the lack of subnucleus level fine mapping system,it still remains unclear that how exactly the V1 cortex neurons are topographically distributed and elegantly organized to target different brain regions to participate specific and precise vision related function.The fine mapping of V1 cortex circuit architecture will be essential for addressing the exact function of visual circuit in vision evoked behavior.Here we constructed a set of recombinant rabies virus expressing different florescent proteins or GCaMP,and applied them to systematically delineate the architecture V1 cortex.By simultaneously retrograde labeling different thalamic nucleus projecting V1 neurons with different florescent rabies virus,we reconstructed topographic pattern of V1 subnucleus.Their corresponding upstream circuits were further explored by mono-trans-synaptic tracing.Notably,significant alterations of V1 projection to thalamic nucleus were observed in monocular deprivation model.Furthermore,we analyzed spontaneous dendritic activity of different subset of V1 neurons projecting to different thalamic nuclei by in vivo calcium imaging and demonstrated the topographic and functional organization of V1 cortex subnucleus.Taken together,our work systematically investigated the topographic pattern,functional organization and plasticity of Vl-thalamus projection and their different upstream circuitry.These results could pave the way to explore the detailed function and computation of Vl-thalamus circuit and understand visual dependent behavior.Meanwhile,our multi-floresences rabies virus retrograde and trans-synaptic tracing strategy might be extensively applied to delineate the detailed architecture of any regime in nervous system and shed a light on the fine map of our brain in sub-nucleus level.
引文