Genome-wide identification of circular RNAs in Arabidopsis thaliana across the lifespan

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• 英文论文题名：Genome-wide identification of circular RNAs in Arabidopsis thaliana across the lifespan
• 论文作者：Gang Chen ; Jiawen Cui ; Li Wang ; Zhaogen Lu ; Biao Jin
• 英文论文作者：Gang Chen ; Jiawen Cui ; Li Wang ; Zhaogen Lu ; Biao Jin ; College of Bioscience and Biotechnology ; Yangzhou University ; College of Horticulture and Plant Protection ; Yangzhou University
• 年：2016
• 作者机构：College of Bioscience and Biotechnology,Yangzhou University;College of Horticulture and Plant Protection,Yangzhou University;
• 英文论文关键词：Circular RNA ; Arabidopsis ; deep sequence ; back-splice
• 会议召开时间：2016-10-09
• 会议录名称：2016年全国植物生物学大会摘要集
• 语种：英文
• 分类号：Q943.2
• 学会代码：ZGZO
• 会议名称：2016年全国植物生物学大会
• 会议地点：中国湖北武汉
• 主办单位：中国遗传学会、中国细胞生物学学会、中国植物学会、中国植物生理与植物分子生物学学会、中国作物学会
• 学会名称：中国植物学会
• 页数：1
• 文件大小：906k
• 原文格式：D
• 会议级别：全国

摘要

Circular RNAs(circ RNAs) are a newly identified class of non-coding RNA with the presence of a covalent bond linking the 3′ and 5′ ends generated by backsplicing. Large amount of circ RNAs has been identified in humans,mammals,fish,insects,worms,fungi,and protists,whereas little is known about plant circ RNAs especially in tissue/developmental-stage-specific expression. Here,we conducted a genome-wide identification of circ RNAs in Arabidopsis thaliana across lifespan and presented the profile of complete circ RNAs via deep sequencing of RNA samples with r RNA and other linear RNA removed. In total,1217 circ RNAs were identified from the mixed samples which covered seven developmental stages in A. thaliana,using the strict threshold of at least two unique backspliced supporting reads. The identified circ RNAs showed a non-random distribution in all of the chromosomes,and most were exonic circ RNAs or located within a transcript. Functional annotation analysis indicated that many circ RNAs were related to photosynthesis. In addition,large proportions of circ RNAs were potential miRNA targets,and almost half of the identified circ RNAs had more than two different miRNA-binding sites,which possibly affecting the post-transcriptional regulation of genes by acting as miRNA sponges. Overall,our data provide an important resource for the emerging circ RNA research in A. thaliana and should contribute to enhanced understanding of circ RNAs in plants.
Circular RNAs(circ RNAs) are a newly identified class of non-coding RNA with the presence of a covalent bond linking the 3′ and 5′ ends generated by backsplicing. Large amount of circ RNAs has been identified in humans,mammals,fish,insects,worms,fungi,and protists,whereas little is known about plant circ RNAs especially in tissue/developmental-stage-specific expression. Here,we conducted a genome-wide identification of circ RNAs in Arabidopsis thaliana across lifespan and presented the profile of complete circ RNAs via deep sequencing of RNA samples with r RNA and other linear RNA removed. In total,1217 circ RNAs were identified from the mixed samples which covered seven developmental stages in A. thaliana,using the strict threshold of at least two unique backspliced supporting reads. The identified circ RNAs showed a non-random distribution in all of the chromosomes,and most were exonic circ RNAs or located within a transcript. Functional annotation analysis indicated that many circ RNAs were related to photosynthesis. In addition,large proportions of circ RNAs were potential miRNA targets,and almost half of the identified circ RNAs had more than two different miRNA-binding sites,which possibly affecting the post-transcriptional regulation of genes by acting as miRNA sponges. Overall,our data provide an important resource for the emerging circ RNA research in A. thaliana and should contribute to enhanced understanding of circ RNAs in plants.

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