An R2R3 MYB transcription factor is critical for cold stress tolerance in apple
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- 英文论文题名：An R2R3 MYB transcription factor is critical for cold stress tolerance in apple
- 论文作者：Yinpeng Xie ; Pengxiang Chen ; Liping Wang ; Yan Yan ; Haiyan Li ; Xuewei Li ; Jidi Xu ; Fengwang Ma ; Qingmei Guan
- 英文论文作者：Yinpeng Xie ; Pengxiang Chen ; Liping Wang ; Yan Yan ; Haiyan Li ; Xuewei Li ; Jidi Xu ; Fengwang Ma ; Qingmei Guan ; College of Horticulture ; Northwest A&F University ; State Key Laboratory of Crop Stress Biology in Arid Areas ; Northwest A&F University
- 年：2016
- 作者机构：College of Horticulture,Northwest A&F University;State Key Laboratory of Crop Stress Biology in Arid Areas,Northwest A&F University;
- 英文论文关键词：Malus × domestica ; Md MYB5523 and Md MYB0970 ; cold stress
- 会议召开时间：2016-10-09
- 会议录名称：2016年全国植物生物学大会摘要集
- 语种：英文
- 分类号：S661.1
- 学会代码：ZGZO
- 会议名称：2016年全国植物生物学大会
- 会议地点：中国湖北武汉
- 主办单位：中国遗传学会、中国细胞生物学学会、中国植物学会、中国植物生理与植物分子生物学学会、中国作物学会
- 学会名称：中国植物学会
- 页数：1
- 文件大小：851k
- 原文格式：D
- 会议级别：全国
摘要
Cold stress is one of the adverse effects on apple distribution and production. However,the molecular mechanisms underlying apple cold stress tolerance are not clear. In this study,we cloned a novel R2R3 MYB transcription factor,Md MYB5523,and its close homolog Md MYB0970 from Golden Delicious(Malus × domestica). Expression of Md MYB5523 and Md MYB0970 was slightly induced by cold stress. Sub-cellular localization revealed that both proteins are localized in the nucleus. With Affinity Purification-Mass Spectrometry(AP-MASS) and yeast two-hybrid screening,we obtained two interacting partners of Md MYB5523 and Md MYB0970,Md HYL1(HYPONASTIC LEAVES 1) and Md SERRATE. Chromatin Immunoprecipitation-PCR showed that Md MYB5523 and Md MYB0970 target genes involved in cold stress,including Md SIZ1 and Md CSP3(COLD SHOCK DOMAIN PROTEIN 3). RNAi lines of Md MYB5523 and Md MYB0970 in Gala-3(Malus × domestica) showed increased sensitivity to cold stress,while over-expression lines of Md MYB5523 or Md MYB0970 showed increased tolerance to cold stress,indicating that Md MYB5523 and Md MYB0970 are two positive regulators of cold stress in apple. Taken together,our results indicate that Md MYB5523 and Md MYB0970 played important roles in cold stress tolerance of apple.
Cold stress is one of the adverse effects on apple distribution and production. However,the molecular mechanisms underlying apple cold stress tolerance are not clear. In this study,we cloned a novel R2R3 MYB transcription factor,Md MYB5523,and its close homolog Md MYB0970 from Golden Delicious(Malus × domestica). Expression of Md MYB5523 and Md MYB0970 was slightly induced by cold stress. Sub-cellular localization revealed that both proteins are localized in the nucleus. With Affinity Purification-Mass Spectrometry(AP-MASS) and yeast two-hybrid screening,we obtained two interacting partners of Md MYB5523 and Md MYB0970,Md HYL1(HYPONASTIC LEAVES 1) and Md SERRATE. Chromatin Immunoprecipitation-PCR showed that Md MYB5523 and Md MYB0970 target genes involved in cold stress,including Md SIZ1 and Md CSP3(COLD SHOCK DOMAIN PROTEIN 3). RNAi lines of Md MYB5523 and Md MYB0970 in Gala-3(Malus × domestica) showed increased sensitivity to cold stress,while over-expression lines of Md MYB5523 or Md MYB0970 showed increased tolerance to cold stress,indicating that Md MYB5523 and Md MYB0970 are two positive regulators of cold stress in apple. Taken together,our results indicate that Md MYB5523 and Md MYB0970 played important roles in cold stress tolerance of apple.
Cold stress is one of the adverse effects on apple distribution and production. However,the molecular mechanisms underlying apple cold stress tolerance are not clear. In this study,we cloned a novel R2R3 MYB transcription factor,Md MYB5523,and its close homolog Md MYB0970 from Golden Delicious(Malus × domestica). Expression of Md MYB5523 and Md MYB0970 was slightly induced by cold stress. Sub-cellular localization revealed that both proteins are localized in the nucleus. With Affinity Purification-Mass Spectrometry(AP-MASS) and yeast two-hybrid screening,we obtained two interacting partners of Md MYB5523 and Md MYB0970,Md HYL1(HYPONASTIC LEAVES 1) and Md SERRATE. Chromatin Immunoprecipitation-PCR showed that Md MYB5523 and Md MYB0970 target genes involved in cold stress,including Md SIZ1 and Md CSP3(COLD SHOCK DOMAIN PROTEIN 3). RNAi lines of Md MYB5523 and Md MYB0970 in Gala-3(Malus × domestica) showed increased sensitivity to cold stress,while over-expression lines of Md MYB5523 or Md MYB0970 showed increased tolerance to cold stress,indicating that Md MYB5523 and Md MYB0970 are two positive regulators of cold stress in apple. Taken together,our results indicate that Md MYB5523 and Md MYB0970 played important roles in cold stress tolerance of apple.
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