摘要
Dendrobium officinale Kimura et Migo(Tie-Pi-Shi-Hu), a precious rare folk medicine has been used for centuries for its multiple bioactivities, including nourishing the stomach, promoting the production of body fluid and enhancing the body immune system. Recently, polysaccharide as the major bioactive substances was focused on. Nevertheless, a few homogeneous polysaccharides had been purified while the fine structures were elucidated. In this study, a homogeneous heteroxylan was newly obtained from alkali-extracted crude polysaccharide. Monosaccharide analysis and NMR data revealed that it composed of arabinose, xylose, glucose and4-O-methylglucuronic acid(4-MGA) as well as trace amount of rhamnose and galactose in a ratio of 9.0: 63.4: 7.0: 15.0: 2.6: 3.0. Combined with the methylation analysis and the 1D and 2D NMR spectra, we found that the backbone of this polysaccharide is 1,4-linked xylan with branches of terminal linked arabinose, terminal-linked galactose, terminal-linked 4-Me O-glucuronic acid and1,4-linked glucose directly or indirectly attached to C-2 position of backbone. The sulfated derivative with DS of about 0.9 was prepared according to the chlorosulfonic acid(CSA)–pyridine method. High performance gel permeation chromatograph(HPGPC) showed a symmetric single peak indicated that the sulfated derivative was a homogeneous polysaccharide. The results of biological activity tests suggested that it could significant disrupt tube formation and inhibit the migration of human microvascular endothelial cells(HMEC-1) in a low concentration dose-dependently without cytotoxity.
Dendrobium officinale Kimura et Migo(Tie-Pi-Shi-Hu), a precious rare folk medicine has been used for centuries for its multiple bioactivities, including nourishing the stomach, promoting the production of body fluid and enhancing the body immune system. Recently, polysaccharide as the major bioactive substances was focused on. Nevertheless, a few homogeneous polysaccharides had been purified while the fine structures were elucidated. In this study, a homogeneous heteroxylan was newly obtained from alkali-extracted crude polysaccharide. Monosaccharide analysis and NMR data revealed that it composed of arabinose, xylose, glucose and4-O-methylglucuronic acid(4-MGA) as well as trace amount of rhamnose and galactose in a ratio of 9.0: 63.4: 7.0: 15.0: 2.6: 3.0. Combined with the methylation analysis and the 1D and 2D NMR spectra, we found that the backbone of this polysaccharide is 1,4-linked xylan with branches of terminal linked arabinose, terminal-linked galactose, terminal-linked 4-Me O-glucuronic acid and1,4-linked glucose directly or indirectly attached to C-2 position of backbone. The sulfated derivative with DS of about 0.9 was prepared according to the chlorosulfonic acid(CSA)–pyridine method. High performance gel permeation chromatograph(HPGPC) showed a symmetric single peak indicated that the sulfated derivative was a homogeneous polysaccharide. The results of biological activity tests suggested that it could significant disrupt tube formation and inhibit the migration of human microvascular endothelial cells(HMEC-1) in a low concentration dose-dependently without cytotoxity.
引文