大鼠灌胃蒙药蓝盆花提取物后血浆中5种黄酮类成分的UHPLC-MS/MS测定及药动学研究

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• 英文论文题名：UHPLC-MS/MS determination and pharmacokinetic study of five flavonoids in rat plasma after oral administration of Scabiosae Comosae Flos extract
• 论文作者：冯育林 ; 欧阳辉 ; 黎田儿 ; 何明珍 ; 李军茂 ; 杨世林
• 英文论文作者：FENG Yu-lin ; OUYANG Hui ; LI Tian-er ; HE Ming-zhen ; LI Jun-mao ; YANG Shi-lin ; Jiangxi University of Traditional Chinese Medicine ; State key laboratory of Innovative Drug and Energy-saving Pharmaceutical Equipments ; Nanchang Institute For Food and Drug Control
• 年：2016
• 作者机构：江西中医药大学;创新药物与高效节能降耗制药设备国家重点实验室;Nanchang Institute For Food and Drug Control;
• 论文关键词：UHPLC-MS/MS ; 蓝盆花 ; 黄酮类成分 ; 药动学研究
• 英文论文关键词：UHPLC-MS/MS ; Scabiosae Comosae Flos extract ; flavonoids ; pharmacokinetic
• 会议召开时间：2016-09-27
• 会议录名称：中国药学会第十三届青年药学科研成果交流会论文集
• 语种：中文
• 分类号：R285.5
• 学会代码：YYWS
• 会议名称：中国药学会第十三届青年药学科研成果交流会
• 会议地点：中国江西南昌
• 主办单位：中国药学会
• 学会名称：中国药学会
• 页数：12
• 文件大小：879k
• 原文格式：O
• 会议级别：全国

摘要

目的:建立一种快速、选择性好、灵敏度高的UHPLC-MS/MS方法用于大鼠灌胃蒙药蓝盆花提取物后血浆中5种黄酮类成分的测定。方法:血浆样品的制备采用一步蛋白沉淀法,样品分析采用月旭C18柱(1.7 mn×100 mn,1.7μm。流动相采用乙腈-0.1%甲酸水梯度洗脱。所有的分析物及内标采取负离子模式多反应监测模式:芹菜素(m/z269.0→117.2),大波斯菊苷(m/z 431.0→268.0),芦丁(m/z 285.0→133.2),木犀草甙(m/z 447.0→285.0),异荭草素(m/z 447.1→296.8),人参皂苷-Rg1(m/z 931.4→637.2)。结果:在线性范围内,所有的分析物表现出良好的线性关系(>0.99)。日内和日间精密度<13.4%,方法准确度在2.3~14.1%之间,所有的数据都符合生物分析的最低要求。样品的平均回收率在75.1%-104.1%之间,基质对样品分析无影响。结论:该方法被成功地应用于大鼠灌胃蒙药蓝盆花提取物后血浆中5种黄酮类成分的UHPLC-MS/MS测定及药动学研究。
Objective A rapid,selective and sensitive method using ultra high performance liquid chromatography tandem mass spectrometry was developed and validated for the determination and pharmacokinetic investigation of 5 flavonoids.Methods Plasma sample preparation was achieved through a one-step liquid-liquid extraction method.Plasma samples were analyzed using a Welch C_(18) column(1.7 mm× 100 mm,1.7 urn) with a line gradient elution using acetonitrile and 0.1%acetic acid aqueous solution as the mobile phase.All analytes and ginsenosides-Rg1 were tested in negative ionization mode using multiple reaction monitoring(MRM) of the transitions at m/z 269.0→117.2(apigenin),m/z 431.0→268.0(cosmosiin),m/z 285.0→133.2(luteolin),m/z 447.0→285.0(luteoloside),m/z 447.1→296.8(isoorientin) and m/z 931.4→637.2(IS),respectively.Results The method was linear for all analytes over the investigated ranges,with all correlation coefficients >0.99.The intra- and inter-day precisions(RSD,%)were <13.4%,and accuracy(RE,%) ranged from 2.3 to 14.1%,whose data were within the acceptable limits.The mean extraction recoveries of the analytes from rat plasma were within 75.1%-104.1%,and no notable matrix effect was observed.Conciution This validated method was successfully applied in pharmacokinetic of five chemical ingredients study after oral administration of Scabiosae Comosae Flos extract.

引文

[1]Ministry of health of PRC,(A separayely published part of Mongolia medicine)(中华人民共和国卫生部部颁标准,蒙药分册),(1998),52.
    [2]Inner Mongolia medicinal materials standard(内蒙古药材标准),Inner Mongolia Science and Tehnollog Press,Chifeng,25(1987)203-206.
    [3]J.L.Wang,K.Liu,D.T.Xu,Rapid micropropagation system in vitro and antioxidant activity of Scabiosa tschiliensis Grunning,Plant Growth Regul,69(2013)305-310.
    [4]J.N.Maa,S.Bolraaa,M.Jia,Q.Q.Hea,CM.Maa,Quantification and Antioxidant and Anti-HCV Activities of the Constituents from the Inflorescences of Scabiosa Comosa and S.tschilliensis,Nat Pro Res,30(2016)590-594.
    [5]Clio C,Konstantia G,Ioanna C.Chemosystematic valueof chemical constituents from Scabiosa hymettia(Dipsacaceae),Chem Biodiv,2(2008)318-323.
    [6]Q.Zheng,K.Koike,L.K.Han,New biologically active triterpenoid saponins from Scabiosa tschiliensis,J Nat Prod,67(2004)604-613.
    [7]E.A.Garaev,L.S.Movsumov,M.L.Isaev,Flavonoids and Oleanolic Acid from Scabiosa Caucasica,Chem Nat Compd,4(2008)520-521.
    [8]Yang C,Xiao Y,Wang Z,et al.UHPLC-ESI-MS/MS determination and pharmacokinetic study of two alkaloid components in rat plasma after oral administration of the extract of Corydalis bungeana Turcz.J Chromatogra B.(2014);960:59-66.
    [9]Sun W,Jiang ZL,Zhou L,et al.Determination and pharmacokinetic study of pirfenidone in rat plasma by UPLC-MS/MS.J Chromatogra B.(2015);981:14-8.
    [10]Parker SL,Lipman J,Roberts JA,et al.A simple LC-MS/MS method using HILIC chromatography for the determination of fosfomycin in plasma and urine:application to a pilot pharmacokinetic study in humans.J Pharmaceut Biomed.(2015);105:39-45.