DNA错配修复蛋白缺失与结直肠癌临床病理相关性及预后研究
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- 英文论文题名：The study of defective DNA mismatch repair protein and colorectal cancer clinical pathological correlation and prognosis
- 论文作者：韩军平 ; 王丽曾 ; 罗文潇 ; 曾俊文 ; 马文霞
- 英文论文作者：Jun-ping HAN ; Li-zeng WANG ; Wen-xiao LUO ; Jun-wen Zeng ; Wen-xia MA ; The Department of Pathology ; Baiyin the First People's Hospital
- 年：2016
- 作者机构：甘肃省白银市第一人民医院病理科;
- 论文关键词：DNA错配修复蛋白 ; 结直肠癌 ; 免疫组化 ; 预后
- 英文论文关键词：defective DNA mismatch repair protein ; immunohistochemical ; colorectal cancer ; prognosis
- 会议召开时间：2016-12-01
- 会议录名称：中国医师进修论文选编
- 语种：中文
- 分类号：R735.34
- 学会代码：BCLK
- 会议名称：2016年12月中国医师进修学术交流会
- 会议地点：中国北京
- 主办单位：中国医师进修编委会
- 学会名称：百川利康（北京）国际医学研究院
- 页数：3
- 文件大小：495k
- 原文格式：D
- 会议级别：全国
摘要
目的:研究DNA错配修复蛋白缺失与结直肠癌临床病理相关性及预后。方法:利用免疫组织化学及HE染色,观察结直肠增生性息肉,腺瘤,伴高级别上皮内瘤变腺瘤,结直肠癌病变组织中MLHl、MSH2、MSH6、PMS2、P53、Ki-67蛋白的表达情况。结果:在结直肠癌中,MSI(MSI-H)占13.9%,MSS肿瘤占86.1%。MSI(MSI-H)与结直肠癌所在部位,肿瘤类型,肿瘤分化程度,肿瘤ki-67增殖指数相关,具有统计学意义;与p53蛋白表达无统计学意义。结论:MSI(MSI-H)主要分布在右半结肠,低分化腺癌,粘液性腺癌,具有高增殖指数的癌组织中。它能够评估结直肠癌的预后,成为林奇综合征的基因诊断标志物。
Purpose The study of defective DNA mismatch repair protein and colorectal cancer clinical pathological correlation and prognosis. Methods Using immunohistochemistry and HE staining to observe MLHl, MSH2 and MSH6 and PMS2, p53, Ki-67 protein expression in colorectal hyperplastic polyps, adenoma, with high grade intraepithelial neoplasia adenoma and colorectal cancer lesions. Results In colorectal cancer, MSI(MSI-H) accounted for 13.9%, MSS accounted for 86.1%. MSI(MSI-H) have statistical significance with colorectal cancer location, tumor type, tumor differentiation, tumor Ki-67 proliferation index,and have statistical significance and no statistical significance with e p53 protein. Conclusion MSI(MSI-H) is mainly distributed in the right colon, poorly differentiated adenocarcinoma, mucinous adenocarcinoma and high proliferation index of carcinoma. It can evaluate the prognosis of colorectal cancer, be a markers of genetic diagnosis in Lynch syndrome.
Purpose The study of defective DNA mismatch repair protein and colorectal cancer clinical pathological correlation and prognosis. Methods Using immunohistochemistry and HE staining to observe MLHl, MSH2 and MSH6 and PMS2, p53, Ki-67 protein expression in colorectal hyperplastic polyps, adenoma, with high grade intraepithelial neoplasia adenoma and colorectal cancer lesions. Results In colorectal cancer, MSI(MSI-H) accounted for 13.9%, MSS accounted for 86.1%. MSI(MSI-H) have statistical significance with colorectal cancer location, tumor type, tumor differentiation, tumor Ki-67 proliferation index,and have statistical significance and no statistical significance with e p53 protein. Conclusion MSI(MSI-H) is mainly distributed in the right colon, poorly differentiated adenocarcinoma, mucinous adenocarcinoma and high proliferation index of carcinoma. It can evaluate the prognosis of colorectal cancer, be a markers of genetic diagnosis in Lynch syndrome.
引文
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[7]Pino MS,Chung DC.Micmsatellite instability in the management of colorectal cancer.Expert Rev Gastroenteml.Hepatol,201 1,5(3):385-399.
[8]Boland CR,Thibodeau SN,Hamilton SR,et a1.A National Cancer Institute Workshop on Micmsatellite Instability for cancer detection and familial predisposition:development of international criteria for the determination of microsatellite instability in colorectal cancer[J].Cancer Res,1998,58(22):5248-5257.
[9]Umar A,Boland CR,Terdiman JP,et a1.Revised Bethesda Guidelines for hereditary nonpolyposis colorectal cancer(Lynch syndrome)and microsatellite instability[J].J Natl Cancer Inst,2004,96(4):261-268.
[10]Poulogiannis G,Frayling IM,Arends MJ.DNA mismatch repair deficiency in sporadic colorectal cancer and Lynch syndrome.Histopathology,2010,56(2):167-179
[11]Lanza G,Gafa R,Santini A,et al.Immunohistochemical test for MLH1 and MLH2 expression predicts clinical outcome in stage II and III colorectal cancer patients[J].J Clinical,2006,24(15):2359-2367.
[12]石雪,迎郑杰.系统筛查微卫星不稳定性结直肠癌的意义和策略[J].中华病理学杂志2015.44(1):9-13.
[13]Fearon ER,Vogelstein B.A genetic model for colorectal tumor genesis.Cell 1990;61:759-767
[14]Park IJ,Kim HC,Kim JS,Yu ES,Yu CS,Kim JC.Correlation between h MLH1/h MSH2 and p53 protein expression in sporadic colorectal cancer.Hepatogastroenterology 2005;52:450-454
[15]Sibel Erdamar,Esra Ucaryilmaz,Gokhan Demir.etl.Importance of Mut L homologue MLH1 and Mut S homologue MSH2 expression in Turkish patients with sporadic colorectal cancer World J Gastroenterology 2007 September 7;13(33):4437-4444.
[2]Cunningham D,Atkin W,Lenz HJ et al.Colorectal cancer[J].Lancet,2010,375(9719):1030-1047.
[3]Boland CR Goel A.Microsatellite instability in colorectal cancer[J].Gastroenterology,2010,36(6):2073-2087.
[4]Jass JR.Classification of colorectal cancer based on correlation of clinical,morphological and molecular features[J].Histopathology,2001,50:113-130.
[5]Hemminki K,Eng C.Clinical genetic counseling for familial cancers requires reliable date on familial cancer risks and gene reaction plants[J].J med genet,2004,41(11):8701-807.
[6]支文雪,石素胜.微卫星不稳定性在结直肠癌中的意义[J].国际肿瘤学杂志2012(39)12:935-938.
[7]Pino MS,Chung DC.Micmsatellite instability in the management of colorectal cancer.Expert Rev Gastroenteml.Hepatol,201 1,5(3):385-399.
[8]Boland CR,Thibodeau SN,Hamilton SR,et a1.A National Cancer Institute Workshop on Micmsatellite Instability for cancer detection and familial predisposition:development of international criteria for the determination of microsatellite instability in colorectal cancer[J].Cancer Res,1998,58(22):5248-5257.
[9]Umar A,Boland CR,Terdiman JP,et a1.Revised Bethesda Guidelines for hereditary nonpolyposis colorectal cancer(Lynch syndrome)and microsatellite instability[J].J Natl Cancer Inst,2004,96(4):261-268.
[10]Poulogiannis G,Frayling IM,Arends MJ.DNA mismatch repair deficiency in sporadic colorectal cancer and Lynch syndrome.Histopathology,2010,56(2):167-179
[11]Lanza G,Gafa R,Santini A,et al.Immunohistochemical test for MLH1 and MLH2 expression predicts clinical outcome in stage II and III colorectal cancer patients[J].J Clinical,2006,24(15):2359-2367.
[12]石雪,迎郑杰.系统筛查微卫星不稳定性结直肠癌的意义和策略[J].中华病理学杂志2015.44(1):9-13.
[13]Fearon ER,Vogelstein B.A genetic model for colorectal tumor genesis.Cell 1990;61:759-767
[14]Park IJ,Kim HC,Kim JS,Yu ES,Yu CS,Kim JC.Correlation between h MLH1/h MSH2 and p53 protein expression in sporadic colorectal cancer.Hepatogastroenterology 2005;52:450-454
[15]Sibel Erdamar,Esra Ucaryilmaz,Gokhan Demir.etl.Importance of Mut L homologue MLH1 and Mut S homologue MSH2 expression in Turkish patients with sporadic colorectal cancer World J Gastroenterology 2007 September 7;13(33):4437-4444.