Adaptive evolution of Trichinella spiralis genes by comparative genomic analysis with related nematodes
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- 英文论文题名：Adaptive evolution of Trichinella spiralis genes by comparative genomic analysis with related nematodes
- 论文作者：Zigang Qu ; Wenhui Li ; Nianzhang Zhang ; Li Li ; Hongbin Yan ; Tingting Li ; Jianmin Cui ; Yang Yang ; Wanzhong Jia ; Baoquan Fu
- 英文论文作者：Zigang Qu ; Wenhui Li ; Nianzhang Zhang ; Li Li ; Hongbin Yan ; Tingting Li ; Jianmin Cui ; Yang Yang ; Wanzhong Jia ; Baoquan Fu ; State Key Laboratory of Veterinary Etiological Biology ; Key Laboratory of Veterinary Public Health of the Ministry of Agriculture ; Key Laboratory of Veterinary Parasitology of Gansu Province ; Lanzhou Veterinary Research Institute ; Chinese Academy of Agricultural Sciences ; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease
- 年：2016
- 作者机构：State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Public Health of the Ministry of Agriculture, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences;Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease;
- 英文论文关键词：T.spiralis ; positive selected genes(PSGs) ; anaerobic environment ; adaptive evolution
- 会议召开时间：2016-08-08
- 会议录名称：中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会论文摘要集
- 英文会议录名称：The Abstracts of the Tenth Symposium for Yong Scientists of China Society of Parasitology
- 语种：英文
- 分类号：R383.15
- 学会代码：JISC
- 会议名称：中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会
- 会议地点：中国四川成都
- 主办单位：中国动物学会寄生虫学专业青年委员会
- 学会名称：中国动物学会寄生虫学专业委员会
- 页数：2
- 文件大小：159k
- 原文格式：D
- 会议级别：全国
摘要
Background: Trichinellosis is a parasitic disease of humans caused by nematodes of the genus Trichinella, and cause morbidity and mortality of human globally. Deciphering processes that drive species diversity and adaptation are key to understanding parasitism and developing control strategies. The aim of the present study was to analyse adaptation evolution of Trichinella spiralis genes by comparative genomic analysis with Brugia malayi, Trichuris suis, Ancylostoma aceylanicum and Caenorhabditis elegans and may serve as a foundation for future investigations to understand parasite anaerobic environment adaptation at molecular levels. Method: CODEML program from the PAML package was used to infer the most likely d N/d S ratio for each pair of sequences, the ones with d N/d S ratio > 1 were considered as positive selected genes. Results: A total of 986 genes were positive selected genes(p-value < 0.01). Enrichment analyses showed that the positive selected genes were significantly enriched into categories including metabolic pathways(biosynthesis of secondary metabolites,microbial metabolism in diverse environments, purine metabolism, pyrimidine metabolism, starch and sucrose metabolism, amino sugar and nucleotide sugar metabolism and carbon metabolism); signaling pathway(c AMP signaling pathway, calcium signaling pathway and c GMP-PKG signaling pathway); cytosolic DNA-sensing pathway; RNA polymerase and neuroactive ligand-receptor interaction etc. Positive selected genes in T. spiralis consisted of SPARC(secreted protein acidic and rich in cysteine) gene that correlated with angiogenesis in nurse-cell complex, chitin synthase domain protein and cuticle collagen family genes, lysosomal aspartic protease and several cysteine protease genes as well as epigenetic-related genes that participated in DNA methylation, immune-modulation genes, energy metabolisms-related genes, differentiation or genomic reprogramming genes, DNA repair protein, cyclin-related genes, respiratory chains related genes genes as well as detoxifation genes. And these genes are of importance for a more general understanding of genome evolution and the acquisition of novel characters. It could be proposed that the process at the beginning be a response of host cells to larval invasion, while the process at a later stage it is reforming or restructuring of host cell processes by larva as well as protein repair anddamage clearance systems are likely to be heavily engaged in re-establishing protein homeostasis. Furthermore, one of the most important questions about the ecology of this nematode is how T. spiralis deals with low oxygen concentrations and the toxicity of host enzymes in nurse-cell complex. And several detoxifation gene products may resist the toxicity of host enzyme. Conclusions: Positive selected genes within T. spiralis were screened and analysis of genes that under positive selection partially explain biological adaptation to anaerobic environment within host. The identified genes may have the potential use in drug and vaccine development.
Background: Trichinellosis is a parasitic disease of humans caused by nematodes of the genus Trichinella, and cause morbidity and mortality of human globally. Deciphering processes that drive species diversity and adaptation are key to understanding parasitism and developing control strategies. The aim of the present study was to analyse adaptation evolution of Trichinella spiralis genes by comparative genomic analysis with Brugia malayi, Trichuris suis, Ancylostoma aceylanicum and Caenorhabditis elegans and may serve as a foundation for future investigations to understand parasite anaerobic environment adaptation at molecular levels. Method: CODEML program from the PAML package was used to infer the most likely d N/d S ratio for each pair of sequences, the ones with d N/d S ratio > 1 were considered as positive selected genes. Results: A total of 986 genes were positive selected genes(p-value < 0.01). Enrichment analyses showed that the positive selected genes were significantly enriched into categories including metabolic pathways(biosynthesis of secondary metabolites,microbial metabolism in diverse environments, purine metabolism, pyrimidine metabolism, starch and sucrose metabolism, amino sugar and nucleotide sugar metabolism and carbon metabolism); signaling pathway(c AMP signaling pathway, calcium signaling pathway and c GMP-PKG signaling pathway); cytosolic DNA-sensing pathway; RNA polymerase and neuroactive ligand-receptor interaction etc. Positive selected genes in T. spiralis consisted of SPARC(secreted protein acidic and rich in cysteine) gene that correlated with angiogenesis in nurse-cell complex, chitin synthase domain protein and cuticle collagen family genes, lysosomal aspartic protease and several cysteine protease genes as well as epigenetic-related genes that participated in DNA methylation, immune-modulation genes, energy metabolisms-related genes, differentiation or genomic reprogramming genes, DNA repair protein, cyclin-related genes, respiratory chains related genes genes as well as detoxifation genes. And these genes are of importance for a more general understanding of genome evolution and the acquisition of novel characters. It could be proposed that the process at the beginning be a response of host cells to larval invasion, while the process at a later stage it is reforming or restructuring of host cell processes by larva as well as protein repair anddamage clearance systems are likely to be heavily engaged in re-establishing protein homeostasis. Furthermore, one of the most important questions about the ecology of this nematode is how T. spiralis deals with low oxygen concentrations and the toxicity of host enzymes in nurse-cell complex. And several detoxifation gene products may resist the toxicity of host enzyme. Conclusions: Positive selected genes within T. spiralis were screened and analysis of genes that under positive selection partially explain biological adaptation to anaerobic environment within host. The identified genes may have the potential use in drug and vaccine development.
Background: Trichinellosis is a parasitic disease of humans caused by nematodes of the genus Trichinella, and cause morbidity and mortality of human globally. Deciphering processes that drive species diversity and adaptation are key to understanding parasitism and developing control strategies. The aim of the present study was to analyse adaptation evolution of Trichinella spiralis genes by comparative genomic analysis with Brugia malayi, Trichuris suis, Ancylostoma aceylanicum and Caenorhabditis elegans and may serve as a foundation for future investigations to understand parasite anaerobic environment adaptation at molecular levels. Method: CODEML program from the PAML package was used to infer the most likely d N/d S ratio for each pair of sequences, the ones with d N/d S ratio > 1 were considered as positive selected genes. Results: A total of 986 genes were positive selected genes(p-value < 0.01). Enrichment analyses showed that the positive selected genes were significantly enriched into categories including metabolic pathways(biosynthesis of secondary metabolites,microbial metabolism in diverse environments, purine metabolism, pyrimidine metabolism, starch and sucrose metabolism, amino sugar and nucleotide sugar metabolism and carbon metabolism); signaling pathway(c AMP signaling pathway, calcium signaling pathway and c GMP-PKG signaling pathway); cytosolic DNA-sensing pathway; RNA polymerase and neuroactive ligand-receptor interaction etc. Positive selected genes in T. spiralis consisted of SPARC(secreted protein acidic and rich in cysteine) gene that correlated with angiogenesis in nurse-cell complex, chitin synthase domain protein and cuticle collagen family genes, lysosomal aspartic protease and several cysteine protease genes as well as epigenetic-related genes that participated in DNA methylation, immune-modulation genes, energy metabolisms-related genes, differentiation or genomic reprogramming genes, DNA repair protein, cyclin-related genes, respiratory chains related genes genes as well as detoxifation genes. And these genes are of importance for a more general understanding of genome evolution and the acquisition of novel characters. It could be proposed that the process at the beginning be a response of host cells to larval invasion, while the process at a later stage it is reforming or restructuring of host cell processes by larva as well as protein repair anddamage clearance systems are likely to be heavily engaged in re-establishing protein homeostasis. Furthermore, one of the most important questions about the ecology of this nematode is how T. spiralis deals with low oxygen concentrations and the toxicity of host enzymes in nurse-cell complex. And several detoxifation gene products may resist the toxicity of host enzyme. Conclusions: Positive selected genes within T. spiralis were screened and analysis of genes that under positive selection partially explain biological adaptation to anaerobic environment within host. The identified genes may have the potential use in drug and vaccine development.
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