HDL对3T3-L1细胞的葡萄糖转运的影响及其机制的研究
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- 英文论文题名：The Effect and Mechanism of HDL on Glucose Transport in 3T3-L1 cells
- 论文作者：付虹 ; 戎有和
- 英文论文作者：Fu Hong ; Rong Youhe ; Affiliated Hospital of Nanjing University of Traditional Chinese Medicine ; Nanjing University of Traditional Chinese Medicine
- 年：2016
- 作者机构：南京中医药大学附属医院;南京中医药大学;
- 论文关键词：高密度脂蛋白 ; 3T3-L1细胞 ; 葡萄糖的转运 ; 胰岛素抵抗
- 英文论文关键词：HDL ; 3T3-L1 cell ; Glucose transport ; Insulin resistant
- 会议召开时间：2016-09-27
- 会议录名称：中国药学会第十三届青年药学科研成果交流会论文集
- 语种：中文
- 分类号：R96
- 学会代码：YYWS
- 会议名称：中国药学会第十三届青年药学科研成果交流会
- 会议地点：中国江西南昌
- 主办单位：中国药学会
- 学会名称：中国药学会
- 页数：12
- 文件大小：2390k
- 原文格式：O
- 会议级别：全国
摘要
目的:探讨HDL对3T3-L1细胞的葡萄糖转运的影响及其机制。方法:通过对3T3-L1成纤维细胞的分化,培养出符合实验要求的3T3-L1脂肪细胞。通过葡萄糖消耗实验和~3H标记的2-脱氧葡萄糖的摄取实验来研究HDL对葡萄糖转运的影响。通过RT-PCR和West-blotting实验来探讨这个过程的机制。结果:HDL可以促进3T3-L1脂肪细胞对葡萄糖的转运和摄取。3T3-L1脂肪细胞对葡萄糖的转运和摄取过程中,蛋白在RNA转录水平上没有增加,而是发生了AKT和AMPK蛋白的磷酸化。结论:HDL促进3T3-L1脂肪细胞对葡萄糖的摄取是通过AKT和AMPK两条途径来实现的。
Object:To investigate the effect and mechanism of HDL on glucose transport in 3T3-L1 cells.Method:3T3-L1 cells were cultured and induced to differentiation and maturation.The effect of HDL on glucose transport was studied by glucose consumption test and[~3H]-2-deoxyglucose glucose uptake experiment.Exploring the mechanism of this effect by West-blotting and RT-PCR experiment.Result:HDL can promote glucose transport and uptake in 3T3-L1 cells.In this process,the protein did not increase at the RNA transcriptional level,but the phosphorylation of AKT and AMPK proteins were appeared.Conclusion:HDL stimulates glucose uptake in 3T3-L1 cells involving PI3K/AKT and AMPK signaling pathways.
Object:To investigate the effect and mechanism of HDL on glucose transport in 3T3-L1 cells.Method:3T3-L1 cells were cultured and induced to differentiation and maturation.The effect of HDL on glucose transport was studied by glucose consumption test and[~3H]-2-deoxyglucose glucose uptake experiment.Exploring the mechanism of this effect by West-blotting and RT-PCR experiment.Result:HDL can promote glucose transport and uptake in 3T3-L1 cells.In this process,the protein did not increase at the RNA transcriptional level,but the phosphorylation of AKT and AMPK proteins were appeared.Conclusion:HDL stimulates glucose uptake in 3T3-L1 cells involving PI3K/AKT and AMPK signaling pathways.
引文
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[16]Ann J1,Lee H,Kim S,Park J,Ha T.The anti-obesity effect of quercetin is mediated by the AMPK and MAPK signaling pathways.Biochem Biophys Res Commun.2008,373(4):545-9
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[27]Jensen TE,Schjerling P,Viollet B,Wojtaszewski JF,Richter EA.AMPK alphal activation is required for stimulation of glucose uptake by twitch contraction,but not by H202,in mouse skeletal muscle.PLoS ONE.2008,3x2102
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[29]Kimura T,Tomura H,Sato K,Ito M,Matsuoka I,Im DS,Kuwabara A,Mogi C,Itoh H,Kurose H,Murakami M,Okajima F.Mechanism and role of high density lipoprotein-induced activation of AMP-activated protein kinase in endothelial cells.J Biol Chem.2010,285(7):4387-4397.
[30]Han L,Yu Y,Sun X,Wang B.Exendin-4 directly improves endothelial dysfunction in isolated aortas from obese rats through the cAMP or AMPK-eNOS pathways.Diabetes Res Clin Pract.2012,97:453-60
[31]Jakobsen SN,Hardie DG,Morrice N,Tornqvist HE.:5'-AMP-activated protein kinase phosphorylates IRS-1 on Ser-789 in mouse C2C12myotubes in response to 5-anunoimidazole-4-carboxarnide riboside.Biol Chem.2001,276(50):46912-46916.
[32]Yamazaki S,Satoh H,Watanabe T.Liraglutide enhances insulin sensitivity by activating AMP-activated protein kinase in male Wistar rats.Endocrinology.2014,155(9):3288-301.
[2]Schmidt AM.Insulin resistance and metabolic syndrome:mechanisms and consequences.Arterioscler.Thromb Vase Biol.2012,32(8):1753.
[3]Rashid S,Genest J.Effect of obesity on high-density lipoprotein metabolism.Obesity.2007,15(12):2875-2888.
[4]Hardy OT,Czech MP,Corvera S.What causes the insulin resistance underlying obesity?Curr Opin Endocrinol Diabetes Obes.2012,19(2):81-87.
[5]Umemoto T1,Han CY,Mitra P Apolipoprotein AI and high-density lipoprotein have anti-inflammatory effects on adipocytes via cholesterol transporters:ATP-binding cassette A-1,ATP-binding cassette G-1,and scavenger receptor B-1.Circ Res.2013,112(10):1345-54.
[6]Zhang Q,Zhang Y,Feng H.High density lipoprotein(HDL)promotes glucose uptake in adipocytes and glycogen synthesis in muscle cells.PLoS One.2011,6(8):e23556
[7]Varma S,Khandelwal RL.Overexpression of Aktl Upregulates Glycogen Synthase Activity and Phosphorylation of mTOR in IRS-1Knockdown HepG2 cells J Cell Biochem.2008,103(5):1424-1437.
[8]Neilsen PM,Pishas KI,Callen DF,Thomas DM.Targeting the p53 Pathway in Ewing Sarcoma.Sarcoma 2011,2011:746939
[9]Pereira BC,da Rocha AL,Pinto AP,Pauli IR,de Moura LP,Mekary RA,de Freitas EC,da Silva AS.Excessive training impairs the insulin signal transduction in mice skeletal muscles.J Endocrinol.2016,230(1):93-104.
[10]Dave S,Kaur NJ,Nanduri R..Dkhar HK,.Kumar A,Gupta.P.Inhibition of adipogenesis and induction of apoptosis and lipolysis by stem bromelain in 3T3-L1 adipocytes,PLoS One.2012,7(1):e30831
[11]Yang JY,Della-Fera MA,Rayalam S,A bati S,Hartzell DL,Park HJ,Baile CA.Enhanced inhibition of adipogenesis and induction of apoptosis in 3T3-L1 adipocytes with combinations of resveratrol and quercetin.Life Sci.2008,82(19-20):1032-9.
[12]Brendan D.Manningl,Lewis C.Cantley2 AKT/PKB Signaling:Navigating Downstream Cell.2007,129(7):1261-1274.
[13]Calera MR,Martinez C,Liu H,Jack AK,Bimbaum MJ,Pilch PF.Insulin increases the association of Akt-2 with Glut4-containing vesicles.J Biol Chem.1998,273(13):7201-7204.
[14]Kadowaki T,Tamemoto H,Tobe K,Terauchi Y,Ueki K,Kaburagi Y,Yamauchi T,Satoh S,Sekihara H,Aizawa S,Yazaki Y.Insulin resistance and growth retardation in mice lacking insulin receptor substrate-1 and identification of insulin receptor substrate-2.Diabet Med.1996,13(9 Suppl 6):S103-108.
[15]Yun H,Lee JH,Park CE,Kim MJ,Min BI,Bae H,Choe W,Kang I,Kim SS,Ha J.Inulin increases glucose transport in C2C12myotubes and HepG2 cells via activation of AMP-activated protein kinase and phosphatidylinositol 3-kinase pathways.J Med Food.2009,12(5):1023-1028.
[16]Ann J1,Lee H,Kim S,Park J,Ha T.The anti-obesity effect of quercetin is mediated by the AMPK and MAPK signaling pathways.Biochem Biophys Res Commun.2008,373(4):545-9
[17]Drew BG,Fidge NH,Gallon-Beaumier G,Kemp BE,Kingwell BA.High-density lipoprotein and apolipoprotein AI increase endothelial NO synthase activity by protein association and multisite phosphorylation.Proc Natl Acad Sci USA.2004,101(18):6999-7004.
[18]Hayashi T,Hirshman MF.Kurth EJ.Winder WW.Goodyear LJ.Evidence for 5'AMP-activated protein kinase mediation of the effect of muscle contraction on glucose transport.Diabetes.1998,47(8):1369-1373.
[19]Vawas D.,Apazidis A.,Saha AK.,Gamble J.,Patel A.,Kemp BE.,Witters,LA.Ruderman NB.Contractioninduced changes in acetyl-CoA carboxylase and 5'-AMP-activated kinase in skeletal muscle.J Biol Chem.1997,272(20):13255-13261.
[20]Andreozzi F,Raciti GA,Nigro C,Mannino GC,Procopio T,Davalli AM,Beguinot F,Sesti G,Miele C,Folli F.The GLP-1 receptor agonists exenatide and liraglutide activate Glucose transport by an AMPK-dependent mechanism.J Transl Med.2016,14(1):229.
[21]Mu J,Brozinick JT,Jr Valladares O,Bucan M,Birnbaum MJ.A role for AMP-activated protein kinase in contraction-and hypoxia-regulated glucose transport in skeletal muscle.Mol Cell.2001,7(5):1085-1094.
[22]Hardie DG,Ross FA,Hawley SA.AMPK:a nutrient and energy sensor that maintains energy homeostasis.Nat Rev Mol Cell Biol.2012,13:251-62.
[23]Bijland S,Mancini SJ,Salt IP.Role of AMP-activated protein kinase in adipose tissue metabolism and inflammation.Clin Sci(Lond).2013,124:491-507.
[24]Musi N,Goodyear LJ.AMP-activated protein kinase and muscle glucose uptake.Acta Physiol Scand.2003,178(4):337-345.
[25]Andrews KL,Moore XL,Chin-Dusting JP.Anti-atherogenic effects of high density lipoprotein on nitric oxide synthesis in the endothelium.Clin Exp Pharmacol Physiol.2010,37(7):736-42
[26]Zachariah Tom R,Garcia-Roves PM,SjSgren RJ,Jiang LQ,Holmstr8m MH,Deshmukh AS,et al.effects of AMPK activation on insulin sensitivity and metabolism in leptin-deficient ob/ob mice.Diabetes.2014,63:1560-7.
[27]Jensen TE,Schjerling P,Viollet B,Wojtaszewski JF,Richter EA.AMPK alphal activation is required for stimulation of glucose uptake by twitch contraction,but not by H202,in mouse skeletal muscle.PLoS ONE.2008,3x2102
[28]Yamaguchi S,Katahira H,Ozawa S,Nakamichi Y,Tanaka T,Shimoyama T,Takahashi K,Yoshimoto K,Imaizumi MO,Nagamatsu S,Ishida H.Activators of AMP-activated protein kinase enhance GLUT4 translocation and its glucose transport activity in 3T3-L1 adipocytes.Am J Physiol Endocrinol Metab.2005,289(4):E643-649.
[29]Kimura T,Tomura H,Sato K,Ito M,Matsuoka I,Im DS,Kuwabara A,Mogi C,Itoh H,Kurose H,Murakami M,Okajima F.Mechanism and role of high density lipoprotein-induced activation of AMP-activated protein kinase in endothelial cells.J Biol Chem.2010,285(7):4387-4397.
[30]Han L,Yu Y,Sun X,Wang B.Exendin-4 directly improves endothelial dysfunction in isolated aortas from obese rats through the cAMP or AMPK-eNOS pathways.Diabetes Res Clin Pract.2012,97:453-60
[31]Jakobsen SN,Hardie DG,Morrice N,Tornqvist HE.:5'-AMP-activated protein kinase phosphorylates IRS-1 on Ser-789 in mouse C2C12myotubes in response to 5-anunoimidazole-4-carboxarnide riboside.Biol Chem.2001,276(50):46912-46916.
[32]Yamazaki S,Satoh H,Watanabe T.Liraglutide enhances insulin sensitivity by activating AMP-activated protein kinase in male Wistar rats.Endocrinology.2014,155(9):3288-301.