Differential Expression of miRNA-125b and Validation of Predicted Target Genes in the Skin Tissue of Fine-wool Sheep and Cashmere Goats

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• 英文论文题名：Differential Expression of miRNA-125b and Validation of Predicted Target Genes in the Skin Tissue of Fine-wool Sheep and Cashmere Goats
• 论文作者：Jianping Li ; Haie Qu ; Yumei Li ; Zhihui Zhao ; Huaizhi Jiang ; Qiaoling Zhang
• 英文论文作者：Jianping Li ; Haie Qu ; Yumei Li ; Zhihui Zhao ; Huaizhi Jiang ; Qiaoling Zhang ; Henan University of Animal Husbandry and Economy ; College of Veterinary Medicine ; Jilin University ; College of Animal Science and Technology ; Jilin University ; College of Animal Science and Technology ; Jilin Agricultural University
• 年：2016
• 作者机构：Henan University of Animal Husbandry and Economy;College of Veterinary Medicine,Jilin University;College of Animal Science and Technology,Jilin University;College of Animal Science and Technology,Jilin Agricultural University;
• 英文论文关键词：Fine-wool sheep ; cashmere goats ; miRNA-125b ; MXD4 ; FGFR2
• 会议召开时间：2016-08-20
• 会议录名称：中国畜牧兽医学会动物解剖及组织胚胎学分会第十九次学术研讨会论文集
• 语种：英文
• 分类号：S827
• 学会代码：ZGXJ
• 会议名称：中国畜牧兽医学会动物解剖及组织胚胎学分会第十九次学术研讨会
• 会议地点：中国河南郑州
• 主办单位：中国畜牧兽医学会动物解剖及组织胚胎学分会
• 学会名称：中国畜牧兽医学会
• 页数：1
• 文件大小：60k
• 原文格式：O
• 会议级别：全国

摘要

MicroRNAs(miRNAs) is a new class of endogenous small non-coding RNA which can negatively regulate gene expression via pairing to 3'-untranslated region(UTR) of targeted mRNAs to degrading targeted mRNA or repressing mRNA translation.This study predicted and validated the target gene of miRNA-125 b in the skin tissue of fine-wool sheep and cashmere goats.The target genes of miR-125 b were predicted by miRGen and DAV1 D software.The MAX Dimerization Protein 4(MXD4) and Fibroblast growth factor receptor 2(FGFR2) were chosen as target genes as they correlated with hair follicle development.The expressions of miR-125 b,MXD4 and FGFR2 were examined by qPCR(Real-time Quantitative PCR) and western blot in skin tissue of fine-wool sheep and cashmere goats and Human Embryonic Kidney(HEK) 293 T cells which overexpressed miR-125 b.The luciferase reporter gene system was used to verify the correlation between miR-125 b and MXD4,FGFR2 gene.The expressions of MXD4 and FGFR2 in the skin of cashmere goats was significantly higher than that in fine-wool sheep(p<0.01) and miR-125 b expression level was on the contrary(p<0.01).The levels of MXD4 and FGFR2 could be down-regulated by miR-125 b in HEK 293 T cells.Furthermore,the target sequences of miR-125 b were existed in 3'UTR of FGFR2 and MXD4 gene.MXD4 and FGFR2 are target genes of miR-125 b and miR-125 b may play a regulatory role in the development of sheep hair follicle through these two target genes.
MicroRNAs(miRNAs) is a new class of endogenous small non-coding RNA which can negatively regulate gene expression via pairing to 3'-untranslated region(UTR) of targeted mRNAs to degrading targeted mRNA or repressing mRNA translation.This study predicted and validated the target gene of miRNA-125 b in the skin tissue of fine-wool sheep and cashmere goats.The target genes of miR-125 b were predicted by miRGen and DAV1 D software.The MAX Dimerization Protein 4(MXD4) and Fibroblast growth factor receptor 2(FGFR2) were chosen as target genes as they correlated with hair follicle development.The expressions of miR-125 b,MXD4 and FGFR2 were examined by qPCR(Real-time Quantitative PCR) and western blot in skin tissue of fine-wool sheep and cashmere goats and Human Embryonic Kidney(HEK) 293 T cells which overexpressed miR-125 b.The luciferase reporter gene system was used to verify the correlation between miR-125 b and MXD4,FGFR2 gene.The expressions of MXD4 and FGFR2 in the skin of cashmere goats was significantly higher than that in fine-wool sheep(p<0.01) and miR-125 b expression level was on the contrary(p<0.01).The levels of MXD4 and FGFR2 could be down-regulated by miR-125 b in HEK 293 T cells.Furthermore,the target sequences of miR-125 b were existed in 3'UTR of FGFR2 and MXD4 gene.MXD4 and FGFR2 are target genes of miR-125 b and miR-125 b may play a regulatory role in the development of sheep hair follicle through these two target genes.

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