两个蕹菜镉积累典型品种的根际土壤化学特征
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- 英文论文题名：Chemical characteristics in the rhizosphere soil of two typical water spinach cultivars differing in Cd accumulation
- 论文作者：龚玉莲 ; 杨中艺
- 英文论文作者：GONG Yu-lian ; YANG Zhong-yi ; Laboratory for Applied Ecology ; Department of Biology ; Guangdong University of Education ; Skate Key Laboratory of Biocontrol ; school of Life Sciences ; Sun Yat-sen University
- 年：2014
- 作者机构：广东第二师范学院生物系应用生态学实验室;中山大学生命科学学院有害生物控制与资源利用国家重点实验室;
- 论文关键词：蕹菜 ; 典型品种 ; 根际 ; 土壤化学特征 ; 低Cd积累
- 英文论文关键词：water spinach(Ipomoea aquatica Forsk.) ; typical cultivars ; rhizosphere ; soil chemical characteristics ; low Cd accumulation
- 会议召开时间：2014-12-13
- 会议录名称：“第四届重金属污染防治及风险评价研讨会”暨重金属污染防治专业委员会2014年学术年会论文集
- 语种：中文
- 分类号：X53
- 学会代码：HJKP
- 会议名称：“第四届重金属污染防治及风险评价研讨会”暨重金属污染防治专业委员会2014年学术年会
- 会议地点：中国江苏盐城
- 主办单位：中国环境科学学会、同济大学
- 学会名称：中国环境科学学会
- 页数：11
- 文件大小：2069k
- 原文格式：D
- 会议级别：全国
摘要
采用根箱试验研究蕹菜典型的低Cd品种(QLQ)和高Cd品种(T308)的根际土壤化学特征.QLQ根际DTPA-Cd含量显著高于T308.QLQ根际pH、氧化还原电位(Eh)显著高于T308,电导率(EC)的表现相反.QLQ根际有机质和水溶性有机质(DOM)含量均高于T308.在污染土壤上,根际低分子量有机酸的组成与含量均存在品种间差异.T308根际检测到乙酸、丙酸、柠檬酸和延胡索酸,而QLQ根际仅检测到柠檬酸和延胡索酸.QLQ根际低分子量有机酸总量(1.93 nmol·g-1DM)极显著低于T308(15.11 nmol·g-1DM).与T308比较,QLQ表现出明显不同的根际土壤化学特征.推测QLQ低Cd积累的根际化学机理可能在于:较高含量的有机质、较低含量的低分子量有机酸及其特异性组成,以及较低的酸化和还原土壤的能力,导致其对土壤Cd的活化能力较低,从而降低植株对Cd的吸收积累.
A rhizobox experiment was conducted to investigate the chemical characteristics in the rhizosphere soils between two typical water spinach cultivars differing in Cd accumulation, QLQ(a low-Cd cultivar) and T308(a high-Cd cultivar). Diethylenetriamine pentacetate acid extractable Cd(DTPA-Cd) concentrations in the rhizosphere soil of QLQ were significantly higher than those of T308(P<0.05). pH and Eh in the rhizosphere soil of QLQ were significantly higher than those of T308(P<0.05). The differences of EC between the test cultivars showed an opposite trend. Contents of organic matter and dissolved organic matter(DOM) in the rhizosphere soil of QLQ were both higher than those of T308. In contaminated soil, the composition and concentration of low molecular weight organic acids in the rhizosphere between the two cultivars were both different. Acetic, propionic, citric and fumaric acids were detectable in the rhizosphere soil of T308, and only citric and fumaric acids were detectable in that of QLQ. The total concentration of low molecular weight organic acids in the rhizosphere soil of QLQ(1.930 nmol·g-1DW) was lower than of T308(15.105 nmol·g-1DW)(P<0.01). Compared with the tested high-Cd cultivar(T308) of water spinach, the chemical characteristics in the rhizosphere soil of the tested low-Cd cultivar(QLQ) were obviously distinct: the relatively high contents of organic matter, the low content and specific composition of low molecular weight organic acids, the relatively moderate acidification of soil, and low ability in soil reduction, which might affect Cd mobilization and reduce Cd accumulation.
A rhizobox experiment was conducted to investigate the chemical characteristics in the rhizosphere soils between two typical water spinach cultivars differing in Cd accumulation, QLQ(a low-Cd cultivar) and T308(a high-Cd cultivar). Diethylenetriamine pentacetate acid extractable Cd(DTPA-Cd) concentrations in the rhizosphere soil of QLQ were significantly higher than those of T308(P<0.05). pH and Eh in the rhizosphere soil of QLQ were significantly higher than those of T308(P<0.05). The differences of EC between the test cultivars showed an opposite trend. Contents of organic matter and dissolved organic matter(DOM) in the rhizosphere soil of QLQ were both higher than those of T308. In contaminated soil, the composition and concentration of low molecular weight organic acids in the rhizosphere between the two cultivars were both different. Acetic, propionic, citric and fumaric acids were detectable in the rhizosphere soil of T308, and only citric and fumaric acids were detectable in that of QLQ. The total concentration of low molecular weight organic acids in the rhizosphere soil of QLQ(1.930 nmol·g-1DW) was lower than of T308(15.105 nmol·g-1DW)(P<0.01). Compared with the tested high-Cd cultivar(T308) of water spinach, the chemical characteristics in the rhizosphere soil of the tested low-Cd cultivar(QLQ) were obviously distinct: the relatively high contents of organic matter, the low content and specific composition of low molecular weight organic acids, the relatively moderate acidification of soil, and low ability in soil reduction, which might affect Cd mobilization and reduce Cd accumulation.
引文
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[23]Wang ZW,Shan XQ,Zhang SZ.Comparison between fractionation and bioavailability of trace elements in rhizosphere and bulk soils.Chemosphere,2002,46:1163-1171
[24]Institute of Soil Science,Chinese Academy of Sciences.Soil physical and chemical analyses.Shanghai:Shanghai Science and Technology Press,1978(in Chinese)
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[27]Ni J-Z(倪进治),Xu J-M(徐建民),Xie Z-M(谢正苗),et al.Contents of WSOC and characteristics of its composition under different fertilization systems.Acta Pedologica Sinica(土壤学报),2003,40:724-730(in Chinese)
[28]Szmigielska AM,Van Rees KCJ,Cie?liński G,et al.Low molecular weight dicarboxylic acids in rhizosphere soil of durum wheat.Journal of Agricultural and Food Chemistry,1996,44:1036-1040
[29]Mc Grath SP,Shen ZG,Zhao FJ.Heavy metal uptake and chemical changes in the rhizosphere of Thlaspi caerulescens and Thlaspi ochroleucum grown in contaminated soils.Plant and Soil,1997,188:153-159
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[33]Zhang F-S(张福锁).The change of rhizosphere p H induced by root and its effects.Chinese Journal of Soil Science(土壤通报),1993,24(1):43-45(in Chinese)
[34]Chen T-B(陈同斌),Huang Z-C(黄泽春),Chen H(陈煌).Effect of DOMs extracted from five solid organic wastes on cadmium adsorption in soils.Acta Scientiae Circumstantiae(环境科学学报),2002,22(2):150-155(in Chinese)
[35]Huang Z-C(黄泽春),Chen T-B(陈同斌),Lei M(雷梅).Environmental effects of dissolved organic matters in terrestrial ecosystems:a review.Acta Ecologica Sinica(生态学报),2002,22(2):259-269(in Chinese)
[36]Wang G(王果),Gu X-G(谷勋刚),Gao S-F(高树芳),et al.Adsorption of copper and cadmium on two soils as affected by water-soluble products of three organic materials.Acta Pedologica Sinica(土壤学报),1999,36(2):179-188(in Chinese)
[37]Li T-Q(李廷强),Zhu E(朱恩),Yang X-E(杨肖娥),et al.Effects of dissolved organic matter derived from hyperaccumulator Sedum alfredii Hance rhizosphere on Zn adsorption and desorption in soil.Chinese Journal of Applied Ecology(应用生态学报),2008,19(4):838-844(in Chinese)
[38]Han F,Shan XQ,Zhang J,et al.Organic acids promote the uptake of lanthanum by barley roots.New Phytologist,2005,165:481-492
[39]Greger M,Landberg T.Role of rhizosphere mechanisms in Cd uptake by various wheat cultivars.Plant and Soil,2008,312:195-205
[40]Jones DL,Darrah PR,Kochian VL.Critical evaluation of organic acid mediated iron dissolution in the rhizosphere and its potential role in root iron uptake.Plant and Soil,1996,180:57-66
[41]de la Rosa G,Peralta-Videa JR,Montes M,et al.Cadmium uptake and translocation in tumbleweed(Salsola kali),a potential Cd-hyperaccumulator desert plant species:ICP/OES and XAS studies.Chemosphere,2004,55:1159-1168
[42]Nigam R,Srivastava S,Prakash S,et al.Cadmium mobilisation and plant availability-the impact of organic acids commonly exuded from roots.Plant and Soil,2001,230:107-113
[43]Hinsinger P,Plassard C,Jaillard B.Rhizosphere:A new frontier for soil biogeochemistry.Journal of Geochemical Exploration,2006,88:210-213
[2]Wei BG,Yang LS.A review of heavy metal contaminations in urban soils,urban road dusts and agricultural soils from china.Microchemical Journal,2010,94:99-107
[3]Guo Z-H(郭朝晖),Song J(宋杰),Chen C(陈彩),et al.Heavy metals contamination for cultivated soils,vegetables and rice in the vicinity of non-ferrous metals industry.Ecology and Environment(生态环境),2007,16(4):1144-1148(in Chinese)
[4]Grant CA,Clarke JM,Duguid S,et al.Selection and breeding of plant cultivars to minimize cadmium accumulation.Science of the Total Environment,2008,390:301-310
[5]Yu H,Wang JL,Fang W,et al.Cadmium accumulation in different rice cultivars and screening for pollution safe cultivars of rice.Science of the Total Environment,2006,370:302-309
[6]Yu H(于辉),Yang Z-Y(杨中艺),Yang Z-J(杨知建),et al.Chemical forms and subcellular and molecular distribution of Cd in two Cd-accumulation rice genotypes.Chinese Journal of Applied Ecology(应用生态学报),2008,19(10):2221-2226(in Chinese)
[7]Hart JJ,Welch RM,Norvell WA,et al.Characterization of cadmium uptake,translocation and storage in near-isogenic lines of durum wheat that differ in grain cadmium concentration.New Phytologist,2006,172:261-271
[8]Kidd P,BarcelóJ,Bernal MP,et al.Trace element behaviour at the root-soil interface:Implications in phytoremediation.Evironmental and Experimental Botany,2009,67:243-259
[9]Wu Q-T(吴启堂),Chen L(陈卢),Wang G-S(王广寿).Differences on Cd uptake and accumulation among rice cultivars and its mechanism.Acta Ecologica Sinica(生态学报),1999,19(1):104-107(in Chinese)
[10]Lu ZW,Zhang Z,Su Y,et al.Cultivar variation in morphological response of peanut roots to cadmium stress and its relation to cadmium accumulation.Ecotoxicology and Environmental Safety,2013,91:147-155
[11]Li TQ,Di ZZ,Islam E,et al.Rhizosphere characteristics of zinc hyperaccumulator Sedum alfredii involved in zinc accumulation.Journal of Hazardous Materials,2011,185:818-823
[12]Wenzel WW,Bunkowski M,Puschenreiter M,et al.Rhizosphere characteristics of indigenously growing nickel hyperaccumulator and excluder plants on serpentine soil.Environmental Pollution,2003,123(1):131-138
[13]Li TQ,Liang CF,Han X,et al.Mobilization of cadmium by dissolved organic matter in the rhizosphere of hyperaccumulator Sedum alfredii.Chemosphere,2013,91:970-976
[14]Liu JG,Qian M,Cai GL,et al.Variations between rice cultivars in root secretion of organic acids and the relationship with plant cadmium uptake.Environmental Geochemistry and Health,2007,29(3):189-195
[15]Cie?liński G,Van Rees KCJ,Szmigielska AM,et al.Low-molecular-weight organic acids in rhizosphere soils of durum wheat and their effect on cadmium bioaccumulation.Plant and Soil,1998,203:109-117
[16]Wan M(万敏),Zhou W(周卫),Lin B(林葆).Low-molecular-weight organic acids in rhizosphere soil and their effect on cadmium accumulation in two cultivars of wheat.Plant Nutrition and Fertilizer Science(植物营养与肥料学报),2003,9(3):331-336(in Chinese)
[17]Gong YL,Yuan JG,Yang ZY,et al.Cadmium and lead accumulation by typical cultivars of water spinach as responding to different soil conditions.Fresenius Environmental Bulletin,2010,19(2):190-197
[18]Wang JL,Yuan JG,Yang ZY,et al.Variation in cadmium accumulation among 30 cultivars and cadmium subcellular distribution in 2 selected cultivars of water spinach(Ipomoea aquatica Forsk.).Journal of Agricultural and Food Chemistry,2009,57:8942-8949
[19]Huang BF,Xin JL,Yang ZY,et al.Suppression subtractive hybridization(SSH)-based method for estimating Cd-induced differences in gene expression at cultivar level and identification of genes induced by Cd in two water spinach cultivars.Journal of Agricultural and Food Chemistry,2009,57:8950-8962
[20]Xin JL,Huang BF,Yang JZ,et al.Role of roots in cadmium accumulation of two water spinach cultivars:reciprocal grafting and histochemical experiments.Plant and Soil,2013,366:425-432
[21]Gong Y-L(龚玉莲),Yang Z-Y(杨中艺).Root morphology of typical cultivars of water spinach involved in Cd absorption and accumulation.Journal of South China Normal University(Natural Science Edition)(华南师范大学学报(自然科学版)),2012,44(3):100-106(in Chinese)
[22]State Environmental Protection Administration.National Standards of the People’s Republic of China(HJ/T 332-2006):Environmental quality evaluation standards for farmland of edible agricultural products.Beijing:China Environmental Science Press,2007(in Chinese)
[23]Wang ZW,Shan XQ,Zhang SZ.Comparison between fractionation and bioavailability of trace elements in rhizosphere and bulk soils.Chemosphere,2002,46:1163-1171
[24]Institute of Soil Science,Chinese Academy of Sciences.Soil physical and chemical analyses.Shanghai:Shanghai Science and Technology Press,1978(in Chinese)
[25]Amacher MC.Methods of Soil Analysis.Part3.Chemical Method:Nickel,cadmium,and lead.Wisconsin:American Society of Agronomy,2001
[26]Lindsay WL,Norvell WA.Development of a DTPA soil test for zinc,iron,manganese,and copper.Soil Science Society of America Journal,1978,42:421-428
[27]Ni J-Z(倪进治),Xu J-M(徐建民),Xie Z-M(谢正苗),et al.Contents of WSOC and characteristics of its composition under different fertilization systems.Acta Pedologica Sinica(土壤学报),2003,40:724-730(in Chinese)
[28]Szmigielska AM,Van Rees KCJ,Cie?liński G,et al.Low molecular weight dicarboxylic acids in rhizosphere soil of durum wheat.Journal of Agricultural and Food Chemistry,1996,44:1036-1040
[29]Mc Grath SP,Shen ZG,Zhao FJ.Heavy metal uptake and chemical changes in the rhizosphere of Thlaspi caerulescens and Thlaspi ochroleucum grown in contaminated soils.Plant and Soil,1997,188:153-159
[30]Kirkham MB.Cadmium in plants on polluted soils:effects of soil factors,hyperaccumulation,and amendments.Geoderma,2006,137:19-32
[31]Zeng FR,Ali S,Zhang HT,et al.The influence of p H and organic matter content in paddy soil on heavy metal availability and their uptake by rice plants.Environmental Pollution,2011,159(1):84-91
[32]Sun Q(孙琴),Wang X-R(王晓蓉),Ding S-M(丁士明).Rhizosphere effects in metal absorption by hyperaccumulators and its research advances.Chinese Journal of Ecology(生态学杂志),2005,24(1):30-36(in Chinese)
[33]Zhang F-S(张福锁).The change of rhizosphere p H induced by root and its effects.Chinese Journal of Soil Science(土壤通报),1993,24(1):43-45(in Chinese)
[34]Chen T-B(陈同斌),Huang Z-C(黄泽春),Chen H(陈煌).Effect of DOMs extracted from five solid organic wastes on cadmium adsorption in soils.Acta Scientiae Circumstantiae(环境科学学报),2002,22(2):150-155(in Chinese)
[35]Huang Z-C(黄泽春),Chen T-B(陈同斌),Lei M(雷梅).Environmental effects of dissolved organic matters in terrestrial ecosystems:a review.Acta Ecologica Sinica(生态学报),2002,22(2):259-269(in Chinese)
[36]Wang G(王果),Gu X-G(谷勋刚),Gao S-F(高树芳),et al.Adsorption of copper and cadmium on two soils as affected by water-soluble products of three organic materials.Acta Pedologica Sinica(土壤学报),1999,36(2):179-188(in Chinese)
[37]Li T-Q(李廷强),Zhu E(朱恩),Yang X-E(杨肖娥),et al.Effects of dissolved organic matter derived from hyperaccumulator Sedum alfredii Hance rhizosphere on Zn adsorption and desorption in soil.Chinese Journal of Applied Ecology(应用生态学报),2008,19(4):838-844(in Chinese)
[38]Han F,Shan XQ,Zhang J,et al.Organic acids promote the uptake of lanthanum by barley roots.New Phytologist,2005,165:481-492
[39]Greger M,Landberg T.Role of rhizosphere mechanisms in Cd uptake by various wheat cultivars.Plant and Soil,2008,312:195-205
[40]Jones DL,Darrah PR,Kochian VL.Critical evaluation of organic acid mediated iron dissolution in the rhizosphere and its potential role in root iron uptake.Plant and Soil,1996,180:57-66
[41]de la Rosa G,Peralta-Videa JR,Montes M,et al.Cadmium uptake and translocation in tumbleweed(Salsola kali),a potential Cd-hyperaccumulator desert plant species:ICP/OES and XAS studies.Chemosphere,2004,55:1159-1168
[42]Nigam R,Srivastava S,Prakash S,et al.Cadmium mobilisation and plant availability-the impact of organic acids commonly exuded from roots.Plant and Soil,2001,230:107-113
[43]Hinsinger P,Plassard C,Jaillard B.Rhizosphere:A new frontier for soil biogeochemistry.Journal of Geochemical Exploration,2006,88:210-213