Biological Control of Sclerotinia Stem Rot of Oilseed Rape Using Bacillus subtilis Strain RSS-1
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- 英文论文题名：Biological Control of Sclerotinia Stem Rot of Oilseed Rape Using Bacillus subtilis Strain RSS-1
- 论文作者：DAI Yu-li ; WU Ya ; ZHENG Ting ; ZHANG Qian-ru ; PAN Yue-min ; ZHANG Hua-jian ; GAO Zhi-mou
- 英文论文作者：DAI Yu-li ; WU Ya ; ZHENG Ting ; ZHANG Qian-ru ; PAN Yue-min ; ZHANG Hua-jian ; GAO Zhi-mou ; Department of Plant Pathology ; Anhui Agricultural University
- 年：2016
- 作者机构：Department of Plant Pathology,Anhui Agricultural University;
- 会议召开时间：2016-08-05
- 会议录名称：中国植物病理学会2016年学术年会论文集
- 英文会议录名称：Proceedings of the Annual Meeting of Chinese Society for Plant Pathology(2016)
- 语种：英文
- 分类号：S435.654
- 学会代码：ZGVS
- 会议名称：中国植物病理学会2016年学术年会
- 会议地点：中国江苏南京
- 主办单位：中国植物病理学会
- 学会名称：中国植物病理学会
- 页数：1
- 文件大小：51k
- 原文格式：O
- 会议级别：全国
摘要
Sclerotinia stem rot,caused by Sclerotinia sclerotiorum(Lib.) de Bary,is one of the most important diseases of oilseed rape(Brassica napus L.) in the world.The bacterial strain RSS-1which was isolated from the rhizospheric soil of oilseed rape,proved to be a useful biocontrol strain for application.Morphology,physiological and biochemical tests and 16 S rDNA analysis demonstrated that it was Bacillus subtilis.RSS-1 showed strong antifungal activity in dual-culture assay.RSS-1 significantly inhibited mycelial growth of S.sclerotiorum by 93.4%(P <0.01).Both RSS-1 cell suspension and cellfree filtrate suppressed sclerotial production by 97%~ 100%.In greenhouse experiments,all three tested concentrations,1×10~6,1×10~7 and 1×10~8 CFU/mL of cell suspension or cell-free culture filtrate significantly reduced sclerotinia stem rot severity of oilseed rape(P < 0.05).The most effective concentration of RSS-1 was 1×10~8 CFU/mL,which reduced the severity by 91.1%.Both pretreatment with RSS-1 and treatment with RSS-1 post inoculation at 24 h significantly reduced(P < 0.05) the sclerotinia stem rot severity of rapeseed,compared with the control.The B.subtilis RSS-1 was more effective in reducing disease severity when applied at 24 h before inoculation with S.sclerotiorum than at 24 h post inoculation with S.sclerotiorum.In greenhouse conditions,the population density of B.subtilis strain RSS-1 on rapeseed leaves significandy decreased(P < 0.05) by 5.55 to 2.10 log units over 6 days.However,the population density of RSS-1 in rhizospheric soil of rapeseed increased by 4.05 to 4.75 log units over 30 days.B.subtilis strain RSS-1 significantly inhibit the polygalacturonases,cellulose activities and oxalic acid accumulation(P < 0.01) during the S.sclerotiorum infection.These experimental results provided evidence that RSS-1 could be an excellent alternative biological resource for biocontrol of rapeseed Sclerotinia stem rot caused by S.sclerotiorum.
Sclerotinia stem rot,caused by Sclerotinia sclerotiorum(Lib.) de Bary,is one of the most important diseases of oilseed rape(Brassica napus L.) in the world.The bacterial strain RSS-1which was isolated from the rhizospheric soil of oilseed rape,proved to be a useful biocontrol strain for application.Morphology,physiological and biochemical tests and 16 S rDNA analysis demonstrated that it was Bacillus subtilis.RSS-1 showed strong antifungal activity in dual-culture assay.RSS-1 significantly inhibited mycelial growth of S.sclerotiorum by 93.4%(P <0.01).Both RSS-1 cell suspension and cellfree filtrate suppressed sclerotial production by 97%~ 100%.In greenhouse experiments,all three tested concentrations,1×10~6,1×10~7 and 1×10~8 CFU/mL of cell suspension or cell-free culture filtrate significantly reduced sclerotinia stem rot severity of oilseed rape(P < 0.05).The most effective concentration of RSS-1 was 1×10~8 CFU/mL,which reduced the severity by 91.1%.Both pretreatment with RSS-1 and treatment with RSS-1 post inoculation at 24 h significantly reduced(P < 0.05) the sclerotinia stem rot severity of rapeseed,compared with the control.The B.subtilis RSS-1 was more effective in reducing disease severity when applied at 24 h before inoculation with S.sclerotiorum than at 24 h post inoculation with S.sclerotiorum.In greenhouse conditions,the population density of B.subtilis strain RSS-1 on rapeseed leaves significandy decreased(P < 0.05) by 5.55 to 2.10 log units over 6 days.However,the population density of RSS-1 in rhizospheric soil of rapeseed increased by 4.05 to 4.75 log units over 30 days.B.subtilis strain RSS-1 significantly inhibit the polygalacturonases,cellulose activities and oxalic acid accumulation(P < 0.01) during the S.sclerotiorum infection.These experimental results provided evidence that RSS-1 could be an excellent alternative biological resource for biocontrol of rapeseed Sclerotinia stem rot caused by S.sclerotiorum.
Sclerotinia stem rot,caused by Sclerotinia sclerotiorum(Lib.) de Bary,is one of the most important diseases of oilseed rape(Brassica napus L.) in the world.The bacterial strain RSS-1which was isolated from the rhizospheric soil of oilseed rape,proved to be a useful biocontrol strain for application.Morphology,physiological and biochemical tests and 16 S rDNA analysis demonstrated that it was Bacillus subtilis.RSS-1 showed strong antifungal activity in dual-culture assay.RSS-1 significantly inhibited mycelial growth of S.sclerotiorum by 93.4%(P <0.01).Both RSS-1 cell suspension and cellfree filtrate suppressed sclerotial production by 97%~ 100%.In greenhouse experiments,all three tested concentrations,1×10~6,1×10~7 and 1×10~8 CFU/mL of cell suspension or cell-free culture filtrate significantly reduced sclerotinia stem rot severity of oilseed rape(P < 0.05).The most effective concentration of RSS-1 was 1×10~8 CFU/mL,which reduced the severity by 91.1%.Both pretreatment with RSS-1 and treatment with RSS-1 post inoculation at 24 h significantly reduced(P < 0.05) the sclerotinia stem rot severity of rapeseed,compared with the control.The B.subtilis RSS-1 was more effective in reducing disease severity when applied at 24 h before inoculation with S.sclerotiorum than at 24 h post inoculation with S.sclerotiorum.In greenhouse conditions,the population density of B.subtilis strain RSS-1 on rapeseed leaves significandy decreased(P < 0.05) by 5.55 to 2.10 log units over 6 days.However,the population density of RSS-1 in rhizospheric soil of rapeseed increased by 4.05 to 4.75 log units over 30 days.B.subtilis strain RSS-1 significantly inhibit the polygalacturonases,cellulose activities and oxalic acid accumulation(P < 0.01) during the S.sclerotiorum infection.These experimental results provided evidence that RSS-1 could be an excellent alternative biological resource for biocontrol of rapeseed Sclerotinia stem rot caused by S.sclerotiorum.
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