不同LED光质处理对莲瓣兰组培苗生长的影响
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- 英文论文题名：Effect of LED in vitro Culture of Cymbidium Lianpan
- 论文作者：杨凤玺 ; 许庆全 ; 朱根发
- 英文论文作者：YANG Feng-xi ; XU Qing-quan ; ZHU Gen-fa ; Guangdong Key Laboratory of Ornamental Plant Germplasm Innovation and Utilization ; Environmental Horticulture Research Institute ; Guangdong Academy of Agricultural Sciences ; Guangdong Key Laboratory of Biotechnology for Plant Development ; College of Life Science ; South China Normal University
- 年：2016
- 作者机构：广东省农业科学院环境园艺研究所广东省园林花卉种质创新与利用重点实验室;华南师范大学生命科学学院广东省植物发育生物工程重点实验室;
- 论文关键词：LED光照 ; 光质 ; 莲瓣兰 ; 组织培养
- 英文论文关键词：Light-emitting diode(LED) ; Light quality ; Cymbidium Lianpan ; Tissue culture
- 会议召开时间：2016-07-19
- 会议录名称：中国观赏园艺研究进展2016
- 语种：中文
- 分类号：S682.31
- 学会代码：EGYP
- 会议名称：2016年中国观赏园艺学术研讨会
- 会议地点：中国湖南长沙
- 主办单位：中国园艺学会观赏园艺专业委员会、国家花卉工程技术研究中心
- 学会名称：中国园艺学会
- 页数：4
- 文件大小：722k
- 原文格式：O
- 会议级别：全国
摘要
本研究以播种的莲瓣兰‘大雪素'萌发后生长14个月的无菌苗为培养材料,探究了不同光质的发光二极管(LED)组合对‘大雪素'组培快繁过程中根状茎增殖,新芽分化及其生根率的影响。结果表明:相对于普通荧光作为光源,450~480nm蓝光照射可显著提高莲瓣兰增殖培养过程中根状茎增殖效率;630nm红光作为光源可显著提高继代培养过程中莲瓣兰新生芽生长速率;而在壮苗生根培养过程中,使用红光和蓝光混合搭配作为光源可促进根长生长,并以红光和蓝光1:3比例处理效果最佳,生根数目较对照组增加近2倍,叶片数目增加30%以上。可见,合理搭配使用LED光源作为光源,对莲瓣兰‘大雪素'的组织培养具有重要意义。
This study examined the effect of the red and blue light-emitting diode(LED) light on the proliferation of the rhizomes,the differentiation of cespitose buds and the rooting rate of the tissue culture seedlings of Cymbidium Lianpan.14-month-old seedlings of Cymbidium Lianpan ' Da xue su ' were used as the explants.The results showed that illuminating of the blue light of 450 — 480 nm significantly improved the rhizome proliferation efficiency during the proliferation culture,compared with common fluorescence.The red light of 630 nm markedly increased the growth rate of the cespitose buds in the process of subculture.However,the mixture of the red and blue light used as the light source facilitated the expansion of the root length,especially when treated with the red and blue light by the proportion of 1:3,with almost 2 times-increasing of the striking roots number compared to the control group.All of our results indicated that the LED illumination system used in proper proportion of red and blue light is very important for the tissue culture of Cymbidium Lianpan.
This study examined the effect of the red and blue light-emitting diode(LED) light on the proliferation of the rhizomes,the differentiation of cespitose buds and the rooting rate of the tissue culture seedlings of Cymbidium Lianpan.14-month-old seedlings of Cymbidium Lianpan ' Da xue su ' were used as the explants.The results showed that illuminating of the blue light of 450 — 480 nm significantly improved the rhizome proliferation efficiency during the proliferation culture,compared with common fluorescence.The red light of 630 nm markedly increased the growth rate of the cespitose buds in the process of subculture.However,the mixture of the red and blue light used as the light source facilitated the expansion of the root length,especially when treated with the red and blue light by the proportion of 1:3,with almost 2 times-increasing of the striking roots number compared to the control group.All of our results indicated that the LED illumination system used in proper proportion of red and blue light is very important for the tissue culture of Cymbidium Lianpan.
引文
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2.Hwang J D,Ko D S.Evaluation of Plant Growth according to the Wavelength Characteristics of the LED Light Source[J].Journal of the Korean Society of Manufacturing Process Engineers,2014,13(5):98-106.
3.Hwang J D,Ko D S.Development of a High Efficient LED System for the Plant Growth[J].Journal of the Korean Society of Manufacturing Process Engineers,2014,13(4):121-129.
4.唐永康,郭双生,艾为党,等.不同比例红蓝LED光照对油麦菜生长发育的影响[J].航天医学与医学工程,2010,23(3):206-212.
5.曹刚,张国斌,郁继华,等.不同光质LED光源对黄瓜苗期生长及叶绿素荧光参数的影响[J].中国农业科学,2013,46(6):1297-1304.
6.邸秀茹,焦学磊,崔瑾,等.新型光源LED辐射的不同光质配比光对菊花组培苗生长的影响[J].植物生理学通讯,2008,44(4):661-664.
7.Tanaka Y,Komine T,Haruyama S,et al.Indoor visible light data transmission system utilizing white LED lights[J].IEICE transactions on communications,2003,86(8):2440-2454.
8.Levan A,Fruchter A,Rhoads J,et al.Infrared and Optical Observations of GRB 030115 and its Extremely Red Host Galaxy.[J].The Astrophysical Journal,2006,647(1):471.
9.Anzelika,Renata,Silva,Pavelas.In vitro cultivation of grape culture under solid-state lighting[J].scientific works of the lithuanian institute of horticulture and Lithuanian university of agriculture,2007,26(3):235-245.
10.任桂萍,王小菁,朱根发.不同光质的LED对蝴蝶兰组织培养增殖及生根的影响[J].植物学报,2016,51(1):81-88.