优化微柱离心法提高脂质体包封率测定的准确性
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- 英文论文题名：Improve the Accuracy to Determine the Encapsulation Efficiency of Drug in Liposomes by Optimizing the Micro-Column Centrifugation Method
- 论文作者：任翔 ; 俞昶乐 ; 吴阳 ; 陈莉 ; 张莉
- 英文论文作者：REN Xiang ; YU Chang-le ; WU Yang ; CHEN Li ; ZHANG Li ; Logistics University of Chinese People's Armed Police Force
- 年：2016
- 作者机构：武警后勤学院救援医学系生药与药剂学教研室;
- 论文关键词：微柱离心法 ; 脂质体 ; 包封率 ; 积雪草苷 ; 过柱率 ; 权重系数
- 英文论文关键词：micro-column ; liposomes ; entrapped efficiency ; asiaticoside ; the recovery rate of liposomes ; weight coefficient
- 会议召开时间：2016-09-27
- 会议录名称：中国药学会第十三届青年药学科研成果交流会论文集
- 语种：中文
- 分类号：R943
- 学会代码：YYWS
- 会议名称：中国药学会第十三届青年药学科研成果交流会
- 会议地点：中国江西南昌
- 主办单位：中国药学会
- 学会名称：中国药学会
- 页数：6
- 文件大小：358k
- 原文格式：O
- 会议级别：全国
摘要
目的:优化脂质体包封率测定的条件,提高脂质体包封率测定结果的准确性。方法:以积雪草苷为模型药,建立HPLC法测定积雪草苷含量;采用薄膜分散法分别制备空白脂质体与载药脂质体,利用微柱离心法分离脂质体与游离药物。分别将空白脂质体与纯化后的载药脂质体以及两者的稀释混悬液过微柱,比较脂质体的过柱率,最终采用权重系数对脂质体的包封率进行修正。结果:在所选定的分离条件下,空白脂质体的过柱率为95%,纯化的载药脂质体的过柱率只有90%,二者存在明显差异(P<0.05);脂质体稀释后,空白脂质体的过柱率无明显变化,而载药脂质体的过柱率则从89.49%降至74.25%。结论:两种测定方法脂质体的过柱率差异较大,采用权重系数修正包封率,测得结果更为准确。
Objective To optimize the conditions for determination of the encapsulation efficiency of liposomes and improve the determination accuracy.Methods:To establish HPLC method for determine the contents of asiaticoside,which was used as the active pharmaceutical ingredient.The liposomes were prepared by the thin film dispersion method and separated from free drug by micro-column centrifugation method.Then,the study compared the recovery rate through micro-column between blank liposomes,and purified liposome swas proceeded,and both of them diluted suspensions were so on,respectively.At last,the weight coefficients was used to correct the encapsulation efficiency.Results:Under the separation conditions,the recovery rate of blank liposomes was 95%while the recovery rate of purified liposomes was only 90%,there was a significant difference(P <0.05);when the two kinds of liposomes were diluted,the recovery rate of blank liposomes has no changes,but the recovery rate of purified liposomes has decreased from 89.49%to 74.25%.Conclusion:It is showed that the recovery rate of liposomes has great differences in the two methods.The encapsulation efficiency would be more accuracy with weight coefficient corrected.
Objective To optimize the conditions for determination of the encapsulation efficiency of liposomes and improve the determination accuracy.Methods:To establish HPLC method for determine the contents of asiaticoside,which was used as the active pharmaceutical ingredient.The liposomes were prepared by the thin film dispersion method and separated from free drug by micro-column centrifugation method.Then,the study compared the recovery rate through micro-column between blank liposomes,and purified liposome swas proceeded,and both of them diluted suspensions were so on,respectively.At last,the weight coefficients was used to correct the encapsulation efficiency.Results:Under the separation conditions,the recovery rate of blank liposomes was 95%while the recovery rate of purified liposomes was only 90%,there was a significant difference(P <0.05);when the two kinds of liposomes were diluted,the recovery rate of blank liposomes has no changes,but the recovery rate of purified liposomes has decreased from 89.49%to 74.25%.Conclusion:It is showed that the recovery rate of liposomes has great differences in the two methods.The encapsulation efficiency would be more accuracy with weight coefficient corrected.
引文
1 David W,Fry T.Courtland White,ef a/.Rapid Separation of Low Molecular Weight Solutes from Liposomes without Dilution[J].Anal Biochem,1978,90(2):809-815.
2王汀,李文秀,邓英杰.微柱离心-药脂比测定脂质体药物包封率[J].沈阳药科大学学报,2008,5(I):10-14.
3于燕燕,赵继会,冯年平,等.微柱离心-HPLC法测定鬼臼毒素醇质体的包封率研究[J].中草药,2010,41(10):1634-1637.
4陈孝储,郭一沙,宋菲,等.微柱离心结合胆固醇浓度变化测定水溶性药物脂质体包封率[J].沈阳药科大学学报,2014,31(11):851-855.
5 Donatella Paolino,Donato Cosco,Felisa Cilurzo,et al.Improved in vitro and in vivo collagen biosynthesis by asiaticoside-loaded ultradeformable vesicles[J].J Control Release,2012,162(1):143-151.
6程晓波,王春玲,苏钰清,等.浊度法与膜材测定法在评价脂质体回收率上的对比研究[J].沈阳药科大学学报,2015,32(2):92-99.
7张灵芝.脂质体制备及其在生物医学中的应用[M].北京医科大学,中国协和医科大学联合出版社,1998.51-53.
8 V.P.Torchilin,V.Weissig.Liposome:APractical Approach[M].北京:化学工业出版社,2007.19.
9殷凡,李三鸣,代奕,等.氟比洛芬脂质体的制备和体外透皮实验[J].沈阳药科大学学报,2006,23(9):553-556.
10 Zhang JA,Anyarambhatla G,Ma L,et al.Development and characterization of a novel Cremophor EL free liposome-based paclitaxel(LEP-ETU)formulation[J].Eur J Pharm Biopharm,2005,59(1):177-187.
11王敏,邓英杰,王娜.微柱离心.HPLC法测定盐酸丁卡因脂质体包封率[J].中国药房,2009,20(28):2226-2228.
2王汀,李文秀,邓英杰.微柱离心-药脂比测定脂质体药物包封率[J].沈阳药科大学学报,2008,5(I):10-14.
3于燕燕,赵继会,冯年平,等.微柱离心-HPLC法测定鬼臼毒素醇质体的包封率研究[J].中草药,2010,41(10):1634-1637.
4陈孝储,郭一沙,宋菲,等.微柱离心结合胆固醇浓度变化测定水溶性药物脂质体包封率[J].沈阳药科大学学报,2014,31(11):851-855.
5 Donatella Paolino,Donato Cosco,Felisa Cilurzo,et al.Improved in vitro and in vivo collagen biosynthesis by asiaticoside-loaded ultradeformable vesicles[J].J Control Release,2012,162(1):143-151.
6程晓波,王春玲,苏钰清,等.浊度法与膜材测定法在评价脂质体回收率上的对比研究[J].沈阳药科大学学报,2015,32(2):92-99.
7张灵芝.脂质体制备及其在生物医学中的应用[M].北京医科大学,中国协和医科大学联合出版社,1998.51-53.
8 V.P.Torchilin,V.Weissig.Liposome:APractical Approach[M].北京:化学工业出版社,2007.19.
9殷凡,李三鸣,代奕,等.氟比洛芬脂质体的制备和体外透皮实验[J].沈阳药科大学学报,2006,23(9):553-556.
10 Zhang JA,Anyarambhatla G,Ma L,et al.Development and characterization of a novel Cremophor EL free liposome-based paclitaxel(LEP-ETU)formulation[J].Eur J Pharm Biopharm,2005,59(1):177-187.
11王敏,邓英杰,王娜.微柱离心.HPLC法测定盐酸丁卡因脂质体包封率[J].中国药房,2009,20(28):2226-2228.