微阵列电极的制备及其在毒性检测中的应用
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- 英文论文题名：Preparation of microelectrode array and its application in toxicity detection
- 论文作者：余登斌 ; 翟俊峰 ; 刘玲 ; 董绍俊
- 英文论文作者：Dengbin Yu ; Junfeng Zhai ; Ling Liu ; Shaojun Dong ; State Key Laboratory of Electroanalytical Chemistry ; Changchun Institute of Applied Chemistry ; Chinese Academy of Science ; University of Chinese Academy of Sciences
- 年：2016
- 作者机构：中国科学院长春应用化学研究所电分析化学国家重点实验室;中国科学院大学;
- 论文关键词：微阵列电极(MEA) ; 毒性检测 ; 电子媒介体 ; 抑制率
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第二十六分会：环境化学
- 语种：中文
- 分类号：X835;O657.1
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第二十六分会 ; 环境化学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：138k
- 原文格式：D
- 会议级别：全国
摘要
基于微生物在毒性环境中呼吸作用受抑制的原理[1],生物毒性快速检测对工作电极提出了很高的要求,而微阵列电极(microelectrode array,MEA)由于保持了单一微电极的特性,又可以获得较大的电流,常规的电化学仪器可以检测其信号,能够满足快速毒性检测的需求[2]。本文制备出了一种全玻璃基的铂MEA,工艺简单,稳定性好,抗干扰性强,耐腐蚀,可以像大电极一样抛光重复使用,寿命长。进一步,我们验证了MEA在生物毒性快速检测中应用的可行性,以2.25 m M的铁氰化钾为电子媒介体,OD600=6的混合微生物为受试体,10 mg/L的Cu~(2+)作为目标毒性物质,样品在37 oC摇床中220 rpm培养1 h,从Fig.1 A:(d和e)可以明显看出,无毒和有毒样品能被所制备的MEA有效区分,抑制率为38.56%,灵敏度高,而且每个样品的检测时间仅仅需要10 s,检测快速,结果与Fig.1(B和C)中对应的样品颜色达到了很好的一致性。
Rapid biological toxicity detection has higher demands for the working electrode based on the principle of that microorganisms respiration is inhibited by toxicant[1],while the MEA meets the needs[2].The as-prepared MEA with glass is a simple,stability,strong anti-interference,corrosion resistance and long life electrode.Further,we verify the feasibility of the application in rapid detection of biological toxicity,among,potassium ferricyanide was used as the electron mediator,mixed microbials were used as the target,and Cu~(2+) was used as a target toxicant.it is clear that non-toxic and toxic samples can be distinguished sensitively and rapidly in the Fig.1 A:(d and e),the inhibition rate was 38.56%,and the detection time of each sample was only 10 s,which results was consistent with the corresponding sample in color from Fig.1(B and C).
Rapid biological toxicity detection has higher demands for the working electrode based on the principle of that microorganisms respiration is inhibited by toxicant[1],while the MEA meets the needs[2].The as-prepared MEA with glass is a simple,stability,strong anti-interference,corrosion resistance and long life electrode.Further,we verify the feasibility of the application in rapid detection of biological toxicity,among,potassium ferricyanide was used as the electron mediator,mixed microbials were used as the target,and Cu~(2+) was used as a target toxicant.it is clear that non-toxic and toxic samples can be distinguished sensitively and rapidly in the Fig.1 A:(d and e),the inhibition rate was 38.56%,and the detection time of each sample was only 10 s,which results was consistent with the corresponding sample in color from Fig.1(B and C).
引文
[1]Pasco,N.F.;Goonerate,R.;Daniel,R.M.;Scott,A.J.Int.J.Environ.An.Ch.2008,88(15):1063.
[2]Yong,D.;Liu,L.;Yu,D.;Dong,S.Anal.Chim.Acta.2011,701(2):164.
[2]Yong,D.;Liu,L.;Yu,D.;Dong,S.Anal.Chim.Acta.2011,701(2):164.