养殖水体磷素转化菌种筛选与应用效果初步探讨
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- 英文论文题名：Preliminary discussion on isolation and application effects of phosphorus transformation bacteria in aquaculture system
- 论文作者：吕鹤 ; 兰时乐 ; 肖调义
- 英文论文作者：LV He ; LAN Shile ; XIAO Tiaoyi ; Hunan Engineering Research Center for Utilization of Characteristics of Aquatic resources ; Hunan agricultural university ; College of Bioscience and Biotechnology ; Hunan agricultural university ; Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province
- 年：2016
- 作者机构：湖南农业大学湖南省特色水产资源利用工程技术研究中心;湖南农业大学生物科学技术学院;水产高效健康生产湖南省协同创新中心;
- 论文关键词：解磷细菌 ; 筛选 ; 活性磷
- 英文论文关键词：phosphate-solubilizing bacteria ; isolation ; active phosphorus
- 会议召开时间：2016-11-02
- 会议录名称：2016年中国水产学会学术年会论文摘要集
- 语种：中文
- 分类号：S917.1
- 学会代码：OGSB
- 会议名称：2016年中国水产学会学术年会
- 会议地点：中国四川成都
- 主办单位：中国水产学会、四川省水产学会
- 学会名称：中国水产学会
- 页数：1
- 文件大小：508k
- 原文格式：D
- 会议级别：全国
摘要
利用选择性培养基,以Ca_3(PO_4)_2和卵磷脂分别作为无机磷培养基和有机磷培养基的唯一磷源,从土壤中分离筛选出2株菌株。形体特征为,淡黄色,短杆,产芽孢和青黑色,短杆。溶磷结果测试表明:经24小时的液体摇床培养,以Ca_3(PO_4)_2作为唯一磷源的培养液中,可溶性磷的含量达到14.87mg/l和13.71mg/l。将两种菌发酵培养48小时,按芽孢杆菌:杆菌=1:2的比例混合后,应用于养殖水体。结果水体的亚硝酸盐含量由0.016mg/l下降为0.0038mg/l;氨氮含量由0.43下降为0.048mg/l;而活性磷的含量变化呈现先升高后下降再升高的趋势,总磷的含量变化呈现先升高后下降的趋势。结果表明解磷细菌能促进水体中难溶性磷酸盐转变为植物可吸收利用的活性磷。
Using Ca_3(PO_4)_2 and lecithin as the only source of phosphorus on selective inorganic phosphorus media and organic phosphorus media respectively, isolated and screened 2phosphate-solubilizing bacterial strains from the soil. The characteristics are pale yellow, short rod,spore production and young black, short rod. The test results of Soluble phosphorus show that:after 24 hours incubation with the inoculum, using Ca_3(PO_4)_2 as the only source of phosphorus,available phosphorus reached 14.87 mg/l and 13.71 mg/l respectively. After 48 hours fermentation cultivation of phosphate-solubilizing bacterial strains, with mixture of the bacillales: bacillus=1:2,applied to aquaculture water. The results show that the nitrite contention fell from 0.016mg/l to0.0038 mg/l; Ammonia nitrogen contention fell from 0.43mg/l to 0.048 mg/l; And trend of active phosphorus contention from rise to decline and to rise again, the total phosphorus contention showed a trend from rise to decline.The results showed that the phosphate-solubilizing bacteria can transform unsoluble phosphate into available forms which plants can absorb in aquaculture water
Using Ca_3(PO_4)_2 and lecithin as the only source of phosphorus on selective inorganic phosphorus media and organic phosphorus media respectively, isolated and screened 2phosphate-solubilizing bacterial strains from the soil. The characteristics are pale yellow, short rod,spore production and young black, short rod. The test results of Soluble phosphorus show that:after 24 hours incubation with the inoculum, using Ca_3(PO_4)_2 as the only source of phosphorus,available phosphorus reached 14.87 mg/l and 13.71 mg/l respectively. After 48 hours fermentation cultivation of phosphate-solubilizing bacterial strains, with mixture of the bacillales: bacillus=1:2,applied to aquaculture water. The results show that the nitrite contention fell from 0.016mg/l to0.0038 mg/l; Ammonia nitrogen contention fell from 0.43mg/l to 0.048 mg/l; And trend of active phosphorus contention from rise to decline and to rise again, the total phosphorus contention showed a trend from rise to decline.The results showed that the phosphate-solubilizing bacteria can transform unsoluble phosphate into available forms which plants can absorb in aquaculture water
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