应用解淀粉芽孢杆菌发酵黄芪培养基的筛选与优化
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- 英文论文题名：Optimizing and Selection on the medium for ferment Radix Astragalus by Bacillus amyloliquefaciens
- 论文作者：史同瑞 ; 侯美如 ; 刘宇 ; 王岩 ; 尹珺伊 ; 周庆民 ; 秦平伟
- 英文论文作者：SHI Tong-rui ; HOU Mei-ru ; LIU Yu ; WANG Yan ; YIN Jun-yi ; ZHOU Qin-min ; Qin Ping-wei ; Heilongjiang Institute of Veterinary Medicine Science
- 年：2016
- 作者机构：黑龙江省兽医科学研究所;
- 论文关键词：黄芪 ; 解淀粉芽孢杆菌 ; 中药发酵 ; 固态发酵培养基
- 英文论文关键词：Radix Astragalus ; Bacillus amyloliquefaciens ; Fermentation of Chinese herbal medicine ; Solid fermentation medium
- 会议召开时间：2016-11-04
- 会议录名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会论文集
- 语种：中文
- 分类号：TQ929
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会
- 会议地点：中国山东青岛
- 主办单位：中国畜牧兽医学会动物微生态学分会
- 学会名称：中国畜牧兽医学会
- 页数：1
- 文件大小：73k
- 原文格式：O
- 会议级别：全国
摘要
为筛选优化解淀粉芽孢杆菌固态发酵黄芪的培养基,以菌生长量为指标,采用单因子试验对菌株适宜发酵培养基成分进行筛选;采用二次正交旋转组合试验设计及响应面法,优化了黄芪固态发酵培养基。结果表明,最适发酵培养基成分为黄芪、黄豆粉和CaCO_3。用其分别替代基础培养基中相应营养物质,然后接种解淀粉芽孢杆菌,置37℃以150 r/min振荡培养24h,细菌菌数分别为6.50×10~9 CFU/mL、5.17×10~9 CFU/mL和5.07×10~9 CFU/mL。优化的固态发酵培养基为:黄芪粉30%、黄豆粉10%、CaCO_3 0.2%、水59.8%,37℃发酵培养72 h,发酵物中活菌数达7.67×10~8 CFU/g。本研究优化的黄芪固态发酵培养基适宜解淀粉芽孢杆菌生长,筛选培养基原材料符合固态发酵产业化生产条件。
In order to screening medium for solid fermenting Radix Astragalus by B.amyloliquefaciens,the effective components of solid fermentation medium was selected by the single factor experiments utilising the viable count as an indicator.The components of solid fermentation medium was optimised by quadratic orthogonal rotation combination design and response surface methodology.The results that the optimum components of medium are Astragalus,Soybean flour and Calcium carbonate.The corresponding nutrients in basal medium was replaced by optimum components,then inoculated culture bacteria under the fermentation conditions at temperature 37℃,150 r/min for 24 h,the viable count of B.amyloliquefaciens were 6.50×10~9 CFU/mL,5.17×10~9 CFU/mL and 5.07×10~9 CFU/mL,respectively.The optimum medium consist of 30%Astragalus,10%Soybean flour,0.2%Calcium carbonate and 59.8%water.The viable count of B.amyloliquefaciens cultured in the optimised medium for 72 h at 37℃ was 7.67×10~8 CFU/g.The optimised solid fermentation medium was suitable for the growth of B.amyloliquefaciens,materials are ideal for industrial production conditions of solid fermentation.
In order to screening medium for solid fermenting Radix Astragalus by B.amyloliquefaciens,the effective components of solid fermentation medium was selected by the single factor experiments utilising the viable count as an indicator.The components of solid fermentation medium was optimised by quadratic orthogonal rotation combination design and response surface methodology.The results that the optimum components of medium are Astragalus,Soybean flour and Calcium carbonate.The corresponding nutrients in basal medium was replaced by optimum components,then inoculated culture bacteria under the fermentation conditions at temperature 37℃,150 r/min for 24 h,the viable count of B.amyloliquefaciens were 6.50×10~9 CFU/mL,5.17×10~9 CFU/mL and 5.07×10~9 CFU/mL,respectively.The optimum medium consist of 30%Astragalus,10%Soybean flour,0.2%Calcium carbonate and 59.8%water.The viable count of B.amyloliquefaciens cultured in the optimised medium for 72 h at 37℃ was 7.67×10~8 CFU/g.The optimised solid fermentation medium was suitable for the growth of B.amyloliquefaciens,materials are ideal for industrial production conditions of solid fermentation.
引文
[1]吕晓静,孟小宾,王小武,等.黄芪多糖的免疫作用机制研究进展[J].中国兽药杂志,2014,48(5):66-69.
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[4]张娟,杨彩梅,曹广添,等.解淀粉芽孢杆菌及其作为益生菌的应用[J].动物营养学报,2014,26(4):863-867.
[5]陈成,崔堂兵,于平儒.一株抗真菌的解淀粉芽孢杆菌的鉴定及其抗菌性研究[J].现代食品科技,2011,27(1):36-39.
[2]张丽霞,高文远,王海洋.微生物技术在中药炮制中的应用[J].中国中药杂志,2012,37(24):3695-3700.
[3]Yoshida S,Hiradate S,Tsukamoto T,et al.Antimicrobial activity of culture filtrate of Bbacillus amyloliquefaciens RC-2isolated from mulberry leaves[J].Biological Control,2001(91):181-187.
[4]张娟,杨彩梅,曹广添,等.解淀粉芽孢杆菌及其作为益生菌的应用[J].动物营养学报,2014,26(4):863-867.
[5]陈成,崔堂兵,于平儒.一株抗真菌的解淀粉芽孢杆菌的鉴定及其抗菌性研究[J].现代食品科技,2011,27(1):36-39.