Effects of Di-n-butyl Phthalate and Diethyl Phthalate on Acetylcholinesterase Activity and Neuro-related Gene Expression in Embryonic Zebrafish
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- 英文论文题名：Effects of Di-n-butyl Phthalate and Diethyl Phthalate on Acetylcholinesterase Activity and Neuro-related Gene Expression in Embryonic Zebrafish
- 论文作者：Hai Xu ; Xiaoling Shao ; Zhen Zhang ; Yanmin Zou ; Xiangyang Wu ; Liuqing Yang
- 英文论文作者：Hai Xu ; Xiaoling Shao ; Zhen Zhang ; Yanmin Zou ; Xiangyang Wu ; Liuqing Yang ; School of the Environment ; Jiangsu University ; School of Chemistry and Chemical Engineering ; Jiangsu University
- 年：2013
- 作者机构：School of the Environment, Jiangsu University;School of Chemistry and Chemical Engineering, Jiangsu University;
- 英文论文关键词：Di-n-butyl phthalate ; Diethyl phthalate ; Developmental neurotoxicity ; Nervous system ; Zebrafish
- 会议召开时间：2013-12-26
- 会议录名称：2013年水资源生态保护与水污染控制研讨会论文集
- 语种：英文
- 分类号：X52;X171.5
- 学会代码：HJKP
- 会议名称：2013年水资源生态保护与水污染控制研讨会
- 会议地点：中国黑龙江哈尔滨
- 主办单位：中国环境科学学会、环境保护部环境规划院、哈尔滨工业大学、哈尔滨师范大学、中国科学院南京地理与湖泊研究所
- 学会名称：中国环境科学学会
- 页数：7
- 文件大小：504k
- 原文格式：D
- 会议级别：全国
摘要
In the present study, zebrafish embryos were used to assess the neurotoxicity of di-n-butyl phthalate(DBP), diethyl phthalate(DEP) and their mixture. Four-hour post-fertilization(hpf) zebrafish embryos were exposed to various concentrations of DBP, DEP and their mixture(DBP-DEP) until 96 hpf. The transcriptions levels of selected neuron-related genes reported as neurotoxicity biomarkers were analyzed. The results showed that transcripts of growth associated protein 43(gap43), embryonic lethal abnormal vision –like 3(elavl3), glial fibrillary acidic protein(gfap), myelin basic protein(mbp), α1-tubulin and neurogenin1(ngn1)were significantly up-regulated after DBP, DEP and DBP-DEP mixture exposure. In addition,acetylcholinesterase(ACh E) activity was significantly inhibited in the embryos. These results indicate that DBP and DEP have the potential neurotoxicity in zebrafish embryos.
In the present study, zebrafish embryos were used to assess the neurotoxicity of di-n-butyl phthalate(DBP), diethyl phthalate(DEP) and their mixture. Four-hour post-fertilization(hpf) zebrafish embryos were exposed to various concentrations of DBP, DEP and their mixture(DBP-DEP) until 96 hpf. The transcriptions levels of selected neuron-related genes reported as neurotoxicity biomarkers were analyzed. The results showed that transcripts of growth associated protein 43(gap43), embryonic lethal abnormal vision –like 3(elavl3), glial fibrillary acidic protein(gfap), myelin basic protein(mbp), α1-tubulin and neurogenin1(ngn1)were significantly up-regulated after DBP, DEP and DBP-DEP mixture exposure. In addition,acetylcholinesterase(ACh E) activity was significantly inhibited in the embryos. These results indicate that DBP and DEP have the potential neurotoxicity in zebrafish embryos.
In the present study, zebrafish embryos were used to assess the neurotoxicity of di-n-butyl phthalate(DBP), diethyl phthalate(DEP) and their mixture. Four-hour post-fertilization(hpf) zebrafish embryos were exposed to various concentrations of DBP, DEP and their mixture(DBP-DEP) until 96 hpf. The transcriptions levels of selected neuron-related genes reported as neurotoxicity biomarkers were analyzed. The results showed that transcripts of growth associated protein 43(gap43), embryonic lethal abnormal vision –like 3(elavl3), glial fibrillary acidic protein(gfap), myelin basic protein(mbp), α1-tubulin and neurogenin1(ngn1)were significantly up-regulated after DBP, DEP and DBP-DEP mixture exposure. In addition,acetylcholinesterase(ACh E) activity was significantly inhibited in the embryos. These results indicate that DBP and DEP have the potential neurotoxicity in zebrafish embryos.
引文
[1]Akamatsu W,Okano HJ,Osumi N,Inoue T,Nakamura S,Sakakibara SI,Miura M,Matsuo N,Darnell RB,Okano H(1999)Mammalian ELAV-like neuronal RNA-binding proteins HuB and HuC promote neuronal development in both the central and the peripheral nervous systems.Proc Natl Acad Sci U.S.A.96:9885-9890
[2]Alm H,Kultima K,Scholz B,Nilsson A,Andren PE,Fex-Svenningsen A,Dencker L,Stigson M(2008)Exposure to brominated flame retardant PBDE-99 affects cytoskeletal protein expression in the neonatal mouse cerebral cortex.Neurotoxicology 29:628-637
[3]Behra M,Cousin X,Bertrand C,Vonesch JL,Biellmann D,Chatonnet A,Str?hle U(2002)Acetylcholinesterase is required for neuronal and muscular development in the zebrafish embryo.Nat Neurosci 5:111-118
[4]Chi J(2009)Phthalate acid esters in Potamogeton crispus L.frome Haihe River,China.Chemosphere 77:48-52
[5]Chung SM,Eckrich M,PerroneB izzozero N,Kohn DT,Furneaux H(1997)The Elav-like proteins bind to a conserved regulatory element in the 3'-untranslated region of GAP-43 mR NA.J Biol Chem 272:6593-6598
[6]Cornell RA,Eisen JS(2002)Delta/Notch signaling promotes formation of zebrafish neural crest by repressing neurogenin 1 function.Development 129:2639-2648
[7]Dijk F,Bergen AAB,Kamphuis W(2007)GAP-43 expression is upregulated in retinal ganglion cells after ischemia/reperfusion-induced damage.Exp Eye Res 84:858-867
[8]El-Fawal HAN,O'Callaghan JP(2008)Autoantibodies to neurotypic and gliotypic proteins as biomarkers of neurotoxicity:Assessment of trimethyltin(TMT).Neurotoxicology 29:109-115
[9]Eng LF,Ghirnikar RS,Lee YL(2000)Glial fibrillary acidic protein:GFAP-thirty-one years(1969-2000).Neurochem Res 25:1439-1451
[10]Engel SM,Miodovnik A,Canfield RL,Zhu C,Silva MJ,Calafat AM,Wolff MS(2010)Prenatal phthalate exposure is associated with childhood behavior and executive functioning.Environ Health Perspct 118:565-571
[11]Fan CY,Cowden J,Simmons SO,Padilla S,Ramabhadran R(2010)Gene expression changes in developing zebrafish as potential markers for rapid developmental neurotoxicity screening.Neurotoxicol Teratol 32:91-98
[12]Fausett BV,Goldman D(2006)A role for alpha 1 tubulin-expressing Muller glia in regeneration of the injured zebrafish retina.J Neurosci 26:6303-6313
[13]Ghorpade N,Mehta V,Khare M,Sinkar P,Krishnan S,Rao CV(2002)Toxicity study of diethyl phthalate on freshwater fish Cirrhina mrigala.Ecotoxicol Envrion Saf 53:255-258
[14]Good PJ(1997)The role of elav-like genes,a conserved family encoding RNA binding proteins,in growth and development.Semin.Cell Dev Biol 8:577-584
[15]Hieber V,Dai XH,Foreman M,Goldman D(1998)Induction of alpha 1-tubulin gene expression during development and regeneration of the fish central nervous system.J Neurobiol 37:429-440
[16]Hoshi H,Ohtsuka T(2009)Adult rats exposed to low-doses of di-n-butyl phthalate during gestation exhibit decreased grooming behavior.Bull Environ Contam Toxicol 83:62-66
[17]Jee JH,Koo JG,Keum YH,Park KH,Choi SH,Kang JC(2009)Effects of dibutyl phthalate and di-ethylhexyl phthalate on acetylcholinesterase activity in bagrid catfish,Pseudobagrus fulvidraco(Richdson).J Appl Ichthyol25:771-775
[18]Ma Q,Chen Z,del Barco Barrantes I,de la Pompa JL,Anderson DJ(1998)Neurogenin1 is essential for the determination of neuronal precursors for proximal cranial sensory ganglia.Neuron 20:469-482
[19]Miller VM,Kahnke T,Neu N,Sanchez-Morrissey SR,Brosch K,Kelsey K,Seegel RF(2010)Developmental PCBexposure induces hypothyroxinemia and sex-specific effects on cerebellum glial protein levels in rats.Int J Dev Neurosci 28:553-560
[20]Oehlmann J,Schulte-Oehlmann U,Kloas W,Jagnytsch O,Lutz I,Kusk KO,Wollenberger L(2009)A critical analysis of the biological impacts of plasticizers on wildlife.Phil Trans R Soc B 364:2047-2062
[21]Pe?alver A,Pocurull E,Borrull F,MarcéRM(2000)Determination of phthalate esters in water samples by solid-phase microextraction and gas chromatography with mass spectrometric detection.J Chromatogr A872:191-201
[22]Staples CA,Peterson DR,Parkerton TF,Adams WJ(1997)The environmental fate of phthalate esters:A literature review.Chemosphere 35:667-749
[23]Zeng F,Cui K,Xie Z,Liu M,Li Y,Lin Y,Zeng Z,Li F(2008)Occurrence of phthalate esters in water and sediment of urban lakes in a subtropical city,Guangzhou,South China.Environ Int 34:372-380
[24]Zhang Z,Zhou K,Bu Y,Shan Z,Liu J,Wu X,Yang L,Chen Z(2012)Detemination of malachite green and crystal violet in envrionmental water using temperature-controlled ionic liquid dispersive liquid-liquid microextraction coupled with high performance liquid chromatography.Anal Me
[2]Alm H,Kultima K,Scholz B,Nilsson A,Andren PE,Fex-Svenningsen A,Dencker L,Stigson M(2008)Exposure to brominated flame retardant PBDE-99 affects cytoskeletal protein expression in the neonatal mouse cerebral cortex.Neurotoxicology 29:628-637
[3]Behra M,Cousin X,Bertrand C,Vonesch JL,Biellmann D,Chatonnet A,Str?hle U(2002)Acetylcholinesterase is required for neuronal and muscular development in the zebrafish embryo.Nat Neurosci 5:111-118
[4]Chi J(2009)Phthalate acid esters in Potamogeton crispus L.frome Haihe River,China.Chemosphere 77:48-52
[5]Chung SM,Eckrich M,PerroneB izzozero N,Kohn DT,Furneaux H(1997)The Elav-like proteins bind to a conserved regulatory element in the 3'-untranslated region of GAP-43 mR NA.J Biol Chem 272:6593-6598
[6]Cornell RA,Eisen JS(2002)Delta/Notch signaling promotes formation of zebrafish neural crest by repressing neurogenin 1 function.Development 129:2639-2648
[7]Dijk F,Bergen AAB,Kamphuis W(2007)GAP-43 expression is upregulated in retinal ganglion cells after ischemia/reperfusion-induced damage.Exp Eye Res 84:858-867
[8]El-Fawal HAN,O'Callaghan JP(2008)Autoantibodies to neurotypic and gliotypic proteins as biomarkers of neurotoxicity:Assessment of trimethyltin(TMT).Neurotoxicology 29:109-115
[9]Eng LF,Ghirnikar RS,Lee YL(2000)Glial fibrillary acidic protein:GFAP-thirty-one years(1969-2000).Neurochem Res 25:1439-1451
[10]Engel SM,Miodovnik A,Canfield RL,Zhu C,Silva MJ,Calafat AM,Wolff MS(2010)Prenatal phthalate exposure is associated with childhood behavior and executive functioning.Environ Health Perspct 118:565-571
[11]Fan CY,Cowden J,Simmons SO,Padilla S,Ramabhadran R(2010)Gene expression changes in developing zebrafish as potential markers for rapid developmental neurotoxicity screening.Neurotoxicol Teratol 32:91-98
[12]Fausett BV,Goldman D(2006)A role for alpha 1 tubulin-expressing Muller glia in regeneration of the injured zebrafish retina.J Neurosci 26:6303-6313
[13]Ghorpade N,Mehta V,Khare M,Sinkar P,Krishnan S,Rao CV(2002)Toxicity study of diethyl phthalate on freshwater fish Cirrhina mrigala.Ecotoxicol Envrion Saf 53:255-258
[14]Good PJ(1997)The role of elav-like genes,a conserved family encoding RNA binding proteins,in growth and development.Semin.Cell Dev Biol 8:577-584
[15]Hieber V,Dai XH,Foreman M,Goldman D(1998)Induction of alpha 1-tubulin gene expression during development and regeneration of the fish central nervous system.J Neurobiol 37:429-440
[16]Hoshi H,Ohtsuka T(2009)Adult rats exposed to low-doses of di-n-butyl phthalate during gestation exhibit decreased grooming behavior.Bull Environ Contam Toxicol 83:62-66
[17]Jee JH,Koo JG,Keum YH,Park KH,Choi SH,Kang JC(2009)Effects of dibutyl phthalate and di-ethylhexyl phthalate on acetylcholinesterase activity in bagrid catfish,Pseudobagrus fulvidraco(Richdson).J Appl Ichthyol25:771-775
[18]Ma Q,Chen Z,del Barco Barrantes I,de la Pompa JL,Anderson DJ(1998)Neurogenin1 is essential for the determination of neuronal precursors for proximal cranial sensory ganglia.Neuron 20:469-482
[19]Miller VM,Kahnke T,Neu N,Sanchez-Morrissey SR,Brosch K,Kelsey K,Seegel RF(2010)Developmental PCBexposure induces hypothyroxinemia and sex-specific effects on cerebellum glial protein levels in rats.Int J Dev Neurosci 28:553-560
[20]Oehlmann J,Schulte-Oehlmann U,Kloas W,Jagnytsch O,Lutz I,Kusk KO,Wollenberger L(2009)A critical analysis of the biological impacts of plasticizers on wildlife.Phil Trans R Soc B 364:2047-2062
[21]Pe?alver A,Pocurull E,Borrull F,MarcéRM(2000)Determination of phthalate esters in water samples by solid-phase microextraction and gas chromatography with mass spectrometric detection.J Chromatogr A872:191-201
[22]Staples CA,Peterson DR,Parkerton TF,Adams WJ(1997)The environmental fate of phthalate esters:A literature review.Chemosphere 35:667-749
[23]Zeng F,Cui K,Xie Z,Liu M,Li Y,Lin Y,Zeng Z,Li F(2008)Occurrence of phthalate esters in water and sediment of urban lakes in a subtropical city,Guangzhou,South China.Environ Int 34:372-380
[24]Zhang Z,Zhou K,Bu Y,Shan Z,Liu J,Wu X,Yang L,Chen Z(2012)Detemination of malachite green and crystal violet in envrionmental water using temperature-controlled ionic liquid dispersive liquid-liquid microextraction coupled with high performance liquid chromatography.Anal Me