The effect of DHEA on apoptosis and cohesin levels in oocytes in aged mice
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- 英文论文题名：The effect of DHEA on apoptosis and cohesin levels in oocytes in aged mice
- 论文作者：Wang Ling
- 英文论文作者：Wang Ling ; Laboratory for Reproductive Immunology ; Hospital & Institute of Obstetrics and Gynecology ; IBS ; Fudan University Shanghai Medical College
- 年：2016
- 作者机构：Laboratory for Reproductive Immunology,Hospital & Institute of Obstetrics and Gynecology,IBS,Fudan University Shanghai Medical College;
- 英文论文关键词：DHEA ; apoptosis ; cohesin ; oocyte ; age ; mice
- 会议召开时间：2016-11-04
- 会议录名称：第十一届全国免疫学学术大会摘要汇编
- 英文会议录名称：Abstracts of 2016 CSI Congress on Immunology
- 语种：英文
- 分类号：R711.75
- 学会代码：IGMD
- 会议名称：第十一届全国免疫学学术大会
- 会议地点：中国安徽合肥
- 主办单位：中国免疫学会
- 学会名称：中国免疫学会
- 页数：2
- 文件大小：383k
- 原文格式：D
- 会议级别：全国
摘要
The quality and quantity of oocytes experienced sharp changes in the ovarian aging process with a decreased of follicle numbers and an increase of aneuploidy. The exhaustion of cohesin complex might be responsible for age-related aneuploidy. The supplement of DHEA can improve the ovarian reserve and reduce the aneuploidy rates, but the relationship between DHEA and ovarian functions especially cohesin levels in oocytes is still unknown. The aim of this study is to evaluate the effect of DHEA supplement on ovarian functions including the follicle numbers and cohesion levels in oocytes. mice at different age were used to gain a systematic view into the apoptosis and cohesin levels in oocytes. 9 months of mice were treated with saline, E2, DHEA, respectively. After 4 w, the ovarian tissues were stained with HE. The amount of follicles in each specific stage were counted and confirmed by immunohistochemistry. TUNEL was used to detect the apoptosis of oocytes. The levels of cohesin subunits-REC8, SMC1β and SMC3 in oocytes were measured by immunofluorescent staining. The numbers of primordial follicles, primary follicles and second follicles declined with age in ovary sections while number of antral follicles rised with age in ovary sections. The supplement of 17β-estradiol and DHEA in aged mice only improved the primary follicle and had little effect on follicles of other stages. The apoptosis index of follicles increased with age, while the medication of DHEA and E2 could decrease the apoptosis index of follicles. The cohesin subunits REC8 and SMC1β experienced age-related loss while DHEA and 17β-estradiol could delay the loss. The supplement of DHEA in aged mice could improve the follicles in primary follicles, suppress the apoptosis of oocytes and delay the decrease of cohesin levels in oocytes in aged mice.
The quality and quantity of oocytes experienced sharp changes in the ovarian aging process with a decreased of follicle numbers and an increase of aneuploidy. The exhaustion of cohesin complex might be responsible for age-related aneuploidy. The supplement of DHEA can improve the ovarian reserve and reduce the aneuploidy rates, but the relationship between DHEA and ovarian functions especially cohesin levels in oocytes is still unknown. The aim of this study is to evaluate the effect of DHEA supplement on ovarian functions including the follicle numbers and cohesion levels in oocytes. mice at different age were used to gain a systematic view into the apoptosis and cohesin levels in oocytes. 9 months of mice were treated with saline, E2, DHEA, respectively. After 4 w, the ovarian tissues were stained with HE. The amount of follicles in each specific stage were counted and confirmed by immunohistochemistry. TUNEL was used to detect the apoptosis of oocytes. The levels of cohesin subunits-REC8, SMC1β and SMC3 in oocytes were measured by immunofluorescent staining. The numbers of primordial follicles, primary follicles and second follicles declined with age in ovary sections while number of antral follicles rised with age in ovary sections. The supplement of 17β-estradiol and DHEA in aged mice only improved the primary follicle and had little effect on follicles of other stages. The apoptosis index of follicles increased with age, while the medication of DHEA and E2 could decrease the apoptosis index of follicles. The cohesin subunits REC8 and SMC1β experienced age-related loss while DHEA and 17β-estradiol could delay the loss. The supplement of DHEA in aged mice could improve the follicles in primary follicles, suppress the apoptosis of oocytes and delay the decrease of cohesin levels in oocytes in aged mice.
The quality and quantity of oocytes experienced sharp changes in the ovarian aging process with a decreased of follicle numbers and an increase of aneuploidy. The exhaustion of cohesin complex might be responsible for age-related aneuploidy. The supplement of DHEA can improve the ovarian reserve and reduce the aneuploidy rates, but the relationship between DHEA and ovarian functions especially cohesin levels in oocytes is still unknown. The aim of this study is to evaluate the effect of DHEA supplement on ovarian functions including the follicle numbers and cohesion levels in oocytes. mice at different age were used to gain a systematic view into the apoptosis and cohesin levels in oocytes. 9 months of mice were treated with saline, E2, DHEA, respectively. After 4 w, the ovarian tissues were stained with HE. The amount of follicles in each specific stage were counted and confirmed by immunohistochemistry. TUNEL was used to detect the apoptosis of oocytes. The levels of cohesin subunits-REC8, SMC1β and SMC3 in oocytes were measured by immunofluorescent staining. The numbers of primordial follicles, primary follicles and second follicles declined with age in ovary sections while number of antral follicles rised with age in ovary sections. The supplement of 17β-estradiol and DHEA in aged mice only improved the primary follicle and had little effect on follicles of other stages. The apoptosis index of follicles increased with age, while the medication of DHEA and E2 could decrease the apoptosis index of follicles. The cohesin subunits REC8 and SMC1β experienced age-related loss while DHEA and 17β-estradiol could delay the loss. The supplement of DHEA in aged mice could improve the follicles in primary follicles, suppress the apoptosis of oocytes and delay the decrease of cohesin levels in oocytes in aged mice.
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