Knock-down of apoptosis inducing factor gene protects endoplasmic reticulum stress-mediated goat granulosa cell apoptosis
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- 英文论文题名：Knock-down of apoptosis inducing factor gene protects endoplasmic reticulum stress-mediated goat granulosa cell apoptosis
- 论文作者：杨迪琦 ; Tingting Jiang ; Pengfei Lin ; Huatao Chen ; Lei Wang ; Nan Wang ; Fan Zhao ; Aihua Wang ; 靳亚平
- 英文论文作者：Diqi Yang ; Tingting Jiang ; Pengfei Lin ; Huatao Chen ; Lei Wang ; Nan Wang ; Fan Zhao ; Aihua Wang ; Yaping Jin ; Key Laboratory of Animal Biotechnology of the Ministry of Agriculture ; Northwest A&F University ; College of Veterinary Medicine ; Northwest A&F University
- 年：2016
- 作者机构：Key Laboratory of Animal Biotechnology of the Ministry of Agriculture,Northwest A&F University;College of Veterinary Medicine,Northwest A&F University;
- 会议召开时间：2016-10-22
- 会议录名称：中国畜牧兽医学会兽医产科学分会第十三次学术研讨会论文集
- 语种：英文
- 分类号：S827
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会兽医产科学分会第十三次学术研讨会
- 会议地点：中国浙江宁波
- 主办单位：中国畜牧兽医学会兽医产科学分会
- 学会名称：中国畜牧兽医学会
- 页数：1
- 文件大小：49k
- 原文格式：O
- 会议级别：全国
摘要
The apoptosis of granulosa cells is the main cause of follicular atresia,and endoplasmic reticulum(ER)stress is involved in the apoptosis of granulosa cells.Apoptosis inducing factor(AIF) mediates caspase-independent apoptosis and causes chromatin condensation and DNA fragmentation,but its role in ER stress-mediated granulosa cell apoptosis during goat follicular atresia remains largely unknown.The aim of this study was to investigate the function of AIF in the apoptosis of goat granulosa cells media~(13) ted by ER stress.The results of immunohistochemical and Western blot analyses demonstrated that AIF was mainly located in granulosa cells,and the expression of AIF significantly increased during follicular atresia.Then,AIF-shRNA recombinant lentiviral vectors were constructed successfully and transfected into hTERT-goat granulosa cells(hTERT-GGCs).Real-time quantitative PCR and Western blot analysis confirmed that AIF was effectively knocked down in hTERT-GGCs.Flow cytometry results showed that the knock-down of AIF in hTERT-GGCs reduced apoptosis due to serum starvation or thapsigargin(Tg)treatment.Additionally,AIF depletion changed the expression of related molecular markers molecules of ER stress under Tg treatment.In conclusion,AIF may serve as a key factor during follicular atresia and AIF depletion protects ER stress-mediated goat granulosa cell apoptosis.
The apoptosis of granulosa cells is the main cause of follicular atresia,and endoplasmic reticulum(ER)stress is involved in the apoptosis of granulosa cells.Apoptosis inducing factor(AIF) mediates caspase-independent apoptosis and causes chromatin condensation and DNA fragmentation,but its role in ER stress-mediated granulosa cell apoptosis during goat follicular atresia remains largely unknown.The aim of this study was to investigate the function of AIF in the apoptosis of goat granulosa cells media~(13) ted by ER stress.The results of immunohistochemical and Western blot analyses demonstrated that AIF was mainly located in granulosa cells,and the expression of AIF significantly increased during follicular atresia.Then,AIF-shRNA recombinant lentiviral vectors were constructed successfully and transfected into hTERT-goat granulosa cells(hTERT-GGCs).Real-time quantitative PCR and Western blot analysis confirmed that AIF was effectively knocked down in hTERT-GGCs.Flow cytometry results showed that the knock-down of AIF in hTERT-GGCs reduced apoptosis due to serum starvation or thapsigargin(Tg)treatment.Additionally,AIF depletion changed the expression of related molecular markers molecules of ER stress under Tg treatment.In conclusion,AIF may serve as a key factor during follicular atresia and AIF depletion protects ER stress-mediated goat granulosa cell apoptosis.
The apoptosis of granulosa cells is the main cause of follicular atresia,and endoplasmic reticulum(ER)stress is involved in the apoptosis of granulosa cells.Apoptosis inducing factor(AIF) mediates caspase-independent apoptosis and causes chromatin condensation and DNA fragmentation,but its role in ER stress-mediated granulosa cell apoptosis during goat follicular atresia remains largely unknown.The aim of this study was to investigate the function of AIF in the apoptosis of goat granulosa cells media~(13) ted by ER stress.The results of immunohistochemical and Western blot analyses demonstrated that AIF was mainly located in granulosa cells,and the expression of AIF significantly increased during follicular atresia.Then,AIF-shRNA recombinant lentiviral vectors were constructed successfully and transfected into hTERT-goat granulosa cells(hTERT-GGCs).Real-time quantitative PCR and Western blot analysis confirmed that AIF was effectively knocked down in hTERT-GGCs.Flow cytometry results showed that the knock-down of AIF in hTERT-GGCs reduced apoptosis due to serum starvation or thapsigargin(Tg)treatment.Additionally,AIF depletion changed the expression of related molecular markers molecules of ER stress under Tg treatment.In conclusion,AIF may serve as a key factor during follicular atresia and AIF depletion protects ER stress-mediated goat granulosa cell apoptosis.
引文