缩骨鲫MSTN基因的克隆与组织表达分析
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- 英文论文题名：Cloning and tissues expression analysis of MSTN gene in Carassius auratus sogu var.
- 论文作者：杨扬 ; 许炎坤 ; 舒琥 ; 李强 ; 蓝召军 ; 刘丽
- 英文论文作者：YANG Yang ; XU Yan-kun ; SHU Hu ; LI Qiang ; LAN Zhao-jun ; LIU Li ; School of Life Science ; Guangzhou University ; Shaoguan Fisheries Research Institute ; School of Marine Science ; Agricultural University of South China
- 年：2016
- 作者机构：广州大学生命科学学院;韶关市水产研究所;华南农业大学海洋学院;
- 论文关键词：缩骨鲫(Carassius auratus sogu var.) ; 克隆 ; 肌肉生长抑制素基因 ; 组织表达分布
- 英文论文关键词：Carassius auratus sogu var ; clone ; myostatin(MSTN) ; tissues expression
- 会议召开时间：2016-11-02
- 会议录名称：2016年中国水产学会学术年会论文摘要集
- 语种：中文
- 分类号：S917.4
- 学会代码：OGSB
- 会议名称：2016年中国水产学会学术年会
- 会议地点：中国四川成都
- 主办单位：中国水产学会、四川省水产学会
- 学会名称：中国水产学会
- 页数：1
- 文件大小：577k
- 原文格式：D
- 会议级别：全国
摘要
采用同源克隆,生物信息学方法及荧光定量PCR(Real-time PCR)对缩骨鲫(Carassius auratus sogu var.)肌肉生长抑制素(myostatin,MSTN)基因进行克隆,分析和组织表达状况研究。结果表明:缩骨鲫基因组MSTN基因全长4 737 bp,含有两个内含子和三个外显子;编码区长度为1 128 bp,编码375个氨基酸,包括信号肽(1aa~23aa),TGF-β前肽保守区域(34aa~256aa),TGF-β功能区(281aa~375aa),保守的RXXR蛋白酶酶切位点以及C-端活性区域9个保守的半胱氨酸残基,这与其他物种MSTN相似。此外,缩骨鲫与鲫鱼(Carassius auratus)的MSTN氨基酸序列同源性最高,达到96.1%。以β-actin为内参基因,荧光定量PCR分析表明,MSTN在肌肉中表达量最高;脑、眼、心脏中次之;在肝胰脏、卵巢、肠和肾脏中微量表达。结果为缩骨鲫肌肉生长和发育的分子机制研究提供基础资料。
The myostatin(MSTN) gene in Carassius auratus sogu var. was cloned and analyzed through homology-based cloning, bioinformatics technology and Real-time PCR. The result revealed that the length of MSTN of C. auratus sogu var. is 4737 bp. It includes 3 exons and 2introns. The length of Coding sequence(CDS) is 1128 bp, the gene code 375 amino acids which contain a signal peptide(1aa~23aa), a conserved propeptide domain(34aa~256aa), a functional domain(281aa~375aa), a RXXR proteolysis site, and nine conserved cysteine residues of C-terminal functional domain; As other species, the protein of MSTN have a TGF-β superfamily domain in C. auratus sogu var.; Compared with other species, the homology of MSTN amino acid sequences between C. auratus sogu var. and C. auratus is closer. Using Real-Time PCR detected MSTN mRNA expression of 9 tissues, the result suggested that the MSTN gene was highly expressed in muscle, second highly expressed in brain, eyes, heart and weakly expressed in ovary,hepatopancreas and kidneys in C. auratus sogu var. These results provide useful information for muscle growth and development of C. auratus sogu var.
The myostatin(MSTN) gene in Carassius auratus sogu var. was cloned and analyzed through homology-based cloning, bioinformatics technology and Real-time PCR. The result revealed that the length of MSTN of C. auratus sogu var. is 4737 bp. It includes 3 exons and 2introns. The length of Coding sequence(CDS) is 1128 bp, the gene code 375 amino acids which contain a signal peptide(1aa~23aa), a conserved propeptide domain(34aa~256aa), a functional domain(281aa~375aa), a RXXR proteolysis site, and nine conserved cysteine residues of C-terminal functional domain; As other species, the protein of MSTN have a TGF-β superfamily domain in C. auratus sogu var.; Compared with other species, the homology of MSTN amino acid sequences between C. auratus sogu var. and C. auratus is closer. Using Real-Time PCR detected MSTN mRNA expression of 9 tissues, the result suggested that the MSTN gene was highly expressed in muscle, second highly expressed in brain, eyes, heart and weakly expressed in ovary,hepatopancreas and kidneys in C. auratus sogu var. These results provide useful information for muscle growth and development of C. auratus sogu var.
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