银杏内生菌发酵产物对苹果中链格孢霉抑菌特性的研究
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- 英文论文题名：The research of ginkgo biloba endophyte fermentation product on antifungal properties of Alternaria sp.
- 论文作者：徐志超 ; 叶淑红
- 英文论文作者：Xu Zhichao ; Ye Shuhong ; College of Food Science ; Dalian Polytechnic University
- 年：2016
- 作者机构：大连工业大学食品学院;
- 论文关键词：银杏内生菌 ; 霉心病 ; 链格孢霉 ; 抑菌机理
- 英文论文关键词：ginkgo biloba endophyte ; mould core ; alternaria sp. ; antifungal mechanism
- 会议召开时间：2016-11-09
- 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
- 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
- 语种：中文
- 分类号：TS201.3
- 学会代码：ZGSP
- 会议名称：中国食品科学技术学会第十三届年会
- 会议地点：中国北京
- 主办单位：中国食品科学技术学会
- 学会名称：中国食品科学技术学会
- 页数：2
- 文件大小：101k
- 原文格式：O
- 会议级别：全国
摘要
链格孢霉是一种能够引起苹果腐烂型霉心病的真菌。感染霉心病的苹果果肉发苦,易腐烂,不能长期储存。本研究从腐烂的苹果中分离筛选出链格孢霉菌株,于28℃下用查氏培养基进行培养,并研究其培养特征、形态特征及生物学特性。从银杏果中分离纯化得到银杏内生菌,用肉汤培养基于28℃下进行培养。研究了银杏内生菌发酵产物对链格孢霉菌丝生长、孢子产生及孢子萌发的抑制作用。同时本研究对经银杏内生菌发酵产物处理的链格孢霉培养液的电导率及链格孢霉细胞膜的通透性进行研究。在实际应用方面,将银杏内生菌发酵产物均匀涂抹于新鲜苹果上,并人工接种链格孢霉,结果表明银杏内生菌发酵产物对链格孢霉有一定的抑制作用。最后,本研究测定了涂抹发酵产物的苹果的成分及细胞结构。
Alternaria sp.is a fungal which can cause mould core of apple.The infacted apple its flesh tasted bitter and can not storage long with totted easily.Alternaria sp.was screened from rotten apple,and Czapek's medium was used in the cultured which temperature was 26℃.In addition,the culture and morphological characters,as well as biological characters of alternaria were investigated.Endophytic strains was screened and purified from ginkgo nut by useing Czapek's medium at28℃.The inhibition of ginkgo biloba endophyte fermentation product on hypha growth,spore production and germination were studied in the paper.The electric conductivity of culture liquid of Alternaria sp.and the permeability of Alternaria sp.cell were studied as well.Fermentation product was spread over the fresh apple which were inoculated in practical applications,the inhibition of the fermentation on was reflect in the paper.Finally,the components and cell structure of apple with fermentation were researched in the study.
Alternaria sp.is a fungal which can cause mould core of apple.The infacted apple its flesh tasted bitter and can not storage long with totted easily.Alternaria sp.was screened from rotten apple,and Czapek's medium was used in the cultured which temperature was 26℃.In addition,the culture and morphological characters,as well as biological characters of alternaria were investigated.Endophytic strains was screened and purified from ginkgo nut by useing Czapek's medium at28℃.The inhibition of ginkgo biloba endophyte fermentation product on hypha growth,spore production and germination were studied in the paper.The electric conductivity of culture liquid of Alternaria sp.and the permeability of Alternaria sp.cell were studied as well.Fermentation product was spread over the fresh apple which were inoculated in practical applications,the inhibition of the fermentation on was reflect in the paper.Finally,the components and cell structure of apple with fermentation were researched in the study.
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