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高效液相色谱测定小黄鱼及酶解液呈味核苷酸含量
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摘要
建立了高效液相色谱法测定三种呈味核苷酸含量的方法,确立了含量测定的标准曲线。三种呈味核苷酸含量在2-225μg/ml范围内呈良好的线性关系;实验验证了方法的重复性、稳定性及回收率,证明方法准确、快速、重现性好。同时测定小黄鱼原液及酶解液中呈味核苷酸的含量的变化,明确了酶解反应对增强小黄鱼中鲜味成分的影响,从而为食品增鲜技术提供更多参考依据。
To assay the content and establish the standard curve of three kinds of flavor nucleotide by high performance liquid chromatography.Three kinds of flavor nucleotide content had good linear relationship within 2-225 μg/ml-The method was showed to be fast,efficient and sensitive by the investigation of the precision,repeatability,stability.And the difference of flavor nucleotides in Small yellow croaker fish and enzymatic hydrolysate showed that fresh flavor was enhanced by the enzymolysis reaction.This paper provides more reference in flavor-enhancer for food.
引文
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