牛奶中黄曲霉毒素M1残留固相萃取-液相色谱质谱检测方法研究
详细信息 查看官网全文
- 英文论文题名：Determination of Aflatoxin Ml Residues in Milk by UPLC and UPLC-MS/MS
- 论文作者：宋志超 ; 陈蔷 ; 杜红鸽 ; 徐坤 ; 马浩轩 ; 张增波
- 英文论文作者：SONG Zhi-chao ; CHEN Qiang ; DU Hong-ge ; XU Kun ; MA Hao-xuan ; ZHANG Zeng-bo ; Henan Province Institute of Veterinary Drug and feed Control ; US International Class of Zhengzhou No.7 middle school
- 年：2016
- 作者机构：河南省兽药饲料监察所;郑州市第七中学中美国际部高二1班;
- 论文关键词：牛奶 ; 黄曲霉毒素M_1 ; 残留 ; 超高效液相色谱 ; 超高效液相色谱-串联质谱
- 英文论文关键词：milk ; aflatoxin M_1 ; residue ; UPLC ; UPLC-MS/MS
- 会议召开时间：2016-09-18
- 会议录名称：中国畜牧兽医学会动物药品学分会第五届全国会员代表大会暨2016年学术年会论文集
- 语种：中文
- 分类号：S859.84;O657.63
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物药品学分会第五届全国会员代表大会暨2016年学术年会
- 会议地点：中国四川成都
- 主办单位：中国畜牧兽医学会动物药品学分会
- 学会名称：中国畜牧兽医学会
- 页数：5
- 文件大小：314k
- 原文格式：O
- 会议级别：全国
摘要
建立了牛奶中黄曲霉毒素M_1(AFM;)残留的固相萃取-超高效液相色谱(UPLC)和超高效液相色谱-串联质谱(UPLC-MS/MS)检测方法。样品经乙腈提取,Plexa固相萃取柱净化,UPLC荧光检测器或UPLC-MS/MS电喷雾正离子多反应监测(MRM)模式检测。AFM_1在0.5~50μg/L的系列浓度范围呈线性相关,牛奶中AFM_1的检测限为0.1μg/kg,定量限为0.2μg/kg。在0.1~0.5μg/kg的添加浓度范围内平均回收均为90%~110%,变异系数均小于10%。结果表明该方法简单快速、灵敏度高、重复性好,适用于牛奶中AFM_1残留检测。
An ultra performance liquid chromatography(UPLC) and ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) method was established for the determination of aflatoxin M_1(AFM_1) residues in milk.The samples were extracted by acetonitrile,and was cleaned up by the Plexa SPE,AFM_1 was detected by HPLC Fluorescence detector or HPLC-MS/MS electrospray ionization in the positive ion multiple reaction mode.The calibration curves of the AFM_1 was linear over the concentration ranges of 0.5~50 μg/L.The limit of detection of AFM_1 was 0.1 μg/kg,and the limit of quantification was 0.2 μg/kg.The control samples were spiked in 0.1~0.5 μg/kg,and the average recoveries ranged from 90%to 110%.The coefficient of variation was less than 10%.This method can be applied for the determination of AFM_1 residues in milk.
An ultra performance liquid chromatography(UPLC) and ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) method was established for the determination of aflatoxin M_1(AFM_1) residues in milk.The samples were extracted by acetonitrile,and was cleaned up by the Plexa SPE,AFM_1 was detected by HPLC Fluorescence detector or HPLC-MS/MS electrospray ionization in the positive ion multiple reaction mode.The calibration curves of the AFM_1 was linear over the concentration ranges of 0.5~50 μg/L.The limit of detection of AFM_1 was 0.1 μg/kg,and the limit of quantification was 0.2 μg/kg.The control samples were spiked in 0.1~0.5 μg/kg,and the average recoveries ranged from 90%to 110%.The coefficient of variation was less than 10%.This method can be applied for the determination of AFM_1 residues in milk.
引文
[1]张东升,赵晓联,赵春城.等黄曲霉毒素M_1的危害、污染现状及检测方法进展[J]中国卫生检验杂志,2004,14(3):266-269
[2]International Agency for Research on Cancer.IARC monographs on the evaluation of cancinogenic risk to humans[M].Lyon:World Health Organization,2002.
[3]Jaimez J,Fente CA,Vazquez BI,et al.Application of the assay of aflatoxins by liquid chromatography with luorescence detection in food analysis[J].J Chromatogr A,2000,882(1):1-10.
[4]Prandini A,Tansini G,Sigolo S,et al.On the occurrence of aflatoxin M1 in milk and dairy products[J].Food Chem Toxicol,2009,47(5):984-991.
[5]马建利,奶牛场黄曲霉毒素的防控[J].中国奶牛,2013,31(10):41-42.
[6]GB 9676-2003乳及乳制品中黄曲霉毒素M_1限量[S].
[7]Pavel Rauch,Ladislav Fukal,Pavel Brezina et al.Interferencess in Radioimmunoassay of Aflatoxins in food and Fodder Samples of Plant Origin[J].J AOAC Int,,1988,71(3):491.
[8]闫磊,李卓,张燕.牛奶中黄曲霉毒素的放射免疫法检测[J]食品研究与开发,2010,31(1):135-137.
[9]廖妍俨,黄家岭.酶联免疫法检测牛奶中黄曲霉毒素M_1的应用[J]贵州化工,2012,37(4):33-35.
[10]王雄,王晶译.用免疫亲和层析法净化、高效液相色谱法测定牛奶和奶粉中黄曲霉毒素M1的含量[J].检验检疫科学,2002,12(2):58-61
[11]林维宣,李继业,田苗.单克隆免疫亲和柱—高效液相色谱法测定牛乳中黄曲霉毒素M1[J].中国乳品工业,2000,28(6):24-26.
[12]GB 5413.37-2010食品安全国家标准乳和乳制品中黄曲霉毒素M_1的测定[S].
[2]International Agency for Research on Cancer.IARC monographs on the evaluation of cancinogenic risk to humans[M].Lyon:World Health Organization,2002.
[3]Jaimez J,Fente CA,Vazquez BI,et al.Application of the assay of aflatoxins by liquid chromatography with luorescence detection in food analysis[J].J Chromatogr A,2000,882(1):1-10.
[4]Prandini A,Tansini G,Sigolo S,et al.On the occurrence of aflatoxin M1 in milk and dairy products[J].Food Chem Toxicol,2009,47(5):984-991.
[5]马建利,奶牛场黄曲霉毒素的防控[J].中国奶牛,2013,31(10):41-42.
[6]GB 9676-2003乳及乳制品中黄曲霉毒素M_1限量[S].
[7]Pavel Rauch,Ladislav Fukal,Pavel Brezina et al.Interferencess in Radioimmunoassay of Aflatoxins in food and Fodder Samples of Plant Origin[J].J AOAC Int,,1988,71(3):491.
[8]闫磊,李卓,张燕.牛奶中黄曲霉毒素的放射免疫法检测[J]食品研究与开发,2010,31(1):135-137.
[9]廖妍俨,黄家岭.酶联免疫法检测牛奶中黄曲霉毒素M_1的应用[J]贵州化工,2012,37(4):33-35.
[10]王雄,王晶译.用免疫亲和层析法净化、高效液相色谱法测定牛奶和奶粉中黄曲霉毒素M1的含量[J].检验检疫科学,2002,12(2):58-61
[11]林维宣,李继业,田苗.单克隆免疫亲和柱—高效液相色谱法测定牛乳中黄曲霉毒素M1[J].中国乳品工业,2000,28(6):24-26.
[12]GB 5413.37-2010食品安全国家标准乳和乳制品中黄曲霉毒素M_1的测定[S].