灵芝的超临界萃取物让皮肤的遗传因子情报发现达到正常化
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- 英文论文题名：Reishi supercritical fluid extracts normalize genetic information expression of skin
- 论文作者：村上祐子 ; 足立浩章 ; 坂井田勉 ; 田中浩 ; 八代洋一 ; 中田悟
- 英文论文作者：Yuhko Murakami ; Hiroaki Adachi ; Tsutomu Sakaida ; Hiroshi Tanaka ; Youichi Yashiro ; Satoru Nakata ; Research Laboratories ; Nippon Menard Cosmetic Co.LTD.
- 年：2016
- 作者机构：日本美伊娜多化妆品株式会社综合研究所;
- 论文关键词：灵芝 ; 超临界萃取物 ; 转座子 ; long interspersed nucleotide element 1(LINE1) ; 转录因子 ; forkhead box O1(FOXO1) ; 胶原蛋白 ; 纤维芽细胞
- 英文论文关键词：Reishi ; supercritical fluid extract ; transposon ; LINE1 ; transcription factor ; FOXO1 ; collagen ; fibroblast
- 会议召开时间：2016-06-15
- 会议录名称：第十一届中国化妆品学术研讨会论文集
- 英文会议录名称：The Proceedings of the 11th Seminar on Cosmetic China
- 语种：中文
- 分类号：Q78;TQ464
- 学会代码：ZGXE
- 会议名称：第十一届中国化妆品学术研讨会
- 会议地点：中国上海
- 主办单位：中国香料香精化妆品工业协会
- 学会名称：中国香料香精化妆品工业协会
- 页数：11
- 文件大小：1849k
- 原文格式：O
- 会议级别：全国
摘要
皮肤的遗传因子情报发现,是通过记录于DNA的遗传因子情报向mRNA转录来进行的,对皮肤的机能维持起着重要的作用。在DNA上存在着遗传因子改变可能的转座子。另外,由DNA向mRNA的转录需要转录因子。因此,转座子和转录因子的变化,对于遗传因子情报发现,以及皮肤的机能都有影响。此次对于通过遗传因子情报发现过程的新的皮肤老化原理,以及自古就被认为是抗老化药材的灵芝超临界萃取物的效果,进行了研究讨论。如果对皮肤纤维芽细胞进行UVB照射,就会发现转座子的其中一种LINE1mRNA的增加,转录因子FOXO1mRNA的减少。这两种变化都会引起胶原蛋白的合成量减少。另一方面,灵芝超临界萃取物能够抑制LINE1mRNA和FOXO1mRNA的变化,恢复胶原蛋白的生成。由此可知,遗传因子情报发现的异常,是皮肤老化的原因之一,灵芝超临界萃取物,能够使皮肤的遗传因子情报发现过程达到正常化,并使皮肤保持年轻。
The genetic information expression of skin which progresses through the transcription of genetic information recorded on DNA to mRNA,plays an important role for the maintenance of skin function.There are fransposons on the DNA,which have a potential for genetic alteration.Also,transcription factors are necessary for transcription from DNA to mRNA.Therefore changes of fransposons and transcription factors have an influence on genetic information expression of skin and also the skin function.In this study,we investigated the new skin aging mechanism focused on the genetic information expression process,and effects of Reishi which was well-known as a traditional crude drug for anti-aging.In UVB-irradiated fibroblasts,mRNA level of LINE1,a kind of transposons,increased,and the level of FOXO1,a transcription factor,decreased.Both changes were followed by the reduction of collagen production.On the other hand,Reishi supercritical fluid extracts inhibited mRNA level change of LINE1 and FOXO1,and improved collagen production.These results demonstrate that Reishi supercritical fluid extracts might normalize the genetic information expression process and maintain youthful skin.
The genetic information expression of skin which progresses through the transcription of genetic information recorded on DNA to mRNA,plays an important role for the maintenance of skin function.There are fransposons on the DNA,which have a potential for genetic alteration.Also,transcription factors are necessary for transcription from DNA to mRNA.Therefore changes of fransposons and transcription factors have an influence on genetic information expression of skin and also the skin function.In this study,we investigated the new skin aging mechanism focused on the genetic information expression process,and effects of Reishi which was well-known as a traditional crude drug for anti-aging.In UVB-irradiated fibroblasts,mRNA level of LINE1,a kind of transposons,increased,and the level of FOXO1,a transcription factor,decreased.Both changes were followed by the reduction of collagen production.On the other hand,Reishi supercritical fluid extracts inhibited mRNA level change of LINE1 and FOXO1,and improved collagen production.These results demonstrate that Reishi supercritical fluid extracts might normalize the genetic information expression process and maintain youthful skin.
引文
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[2]Cordaux R,Batzer MA.:The impact of retrotransposons on human genome evolution.[J].Nat.Rev.Genet,2009,10(10):691-703.
[3]Brunet A,Bonni A,Zigmond MJ,Lin MZ,Juo P,Hu LS,Anderson MJ,Arden KC,Blenis J,Greenberg ME.:Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor.[J].Cell,1999,96(6):857-868.
[4]Liang ZE,Yi YJ,Guo YT,Wang RC,Hu QL,Xiong XY:Inhibition of migration and induction of apoptosis in LoVo human colon cancer cells by polysaccharides from Ganoderma lucidum.[J].Mol.Med.Rep.,2015,12(5):7629-7636.
[5]Tao J,Feng KY:Experimental and clinical studies on inhibitory effect of ganoderma lucidum on platelet aggregation.[J].J.Tongji Med.Univ.,1990,10(4):240-243.
[6]#12
[7]Okudaira N,Iijima K,Koyama T,Minemoto Y,Kano S,Mimori A,Ishizaka Y:Induction of long interspersed nucleotide element-1(L1)retrotransposition by6-formylindolo[3,2-b]carbazole(FICZ),a tryptophan photoproduct.[J].Proc.Natl.Acad.Sci.U.S.A.,2010,107(43):18487-18492.
[8]Banerjee G,Gupta N,Tiwari J,Raman G.:Ultraviolet-induced transformation of keratinocytes:possible involvement of long interspersed element-1 reverse transcriptase.[J].Photodermatol.Photoimmunol.Photomed.,2005,21(1):32-39.
[9]#12
[10]Kato Y,Kaneda M,Hata K,Kumaki K,Hisano M,Kohara Y,Okano M,Li E,Nozaki M,Sasaki H.:Role of the Dnmt3 family in de novo methylation of imprinted and repetitive sequences during male germ cell development in the mouse.[J].Hum.Mol.Genet,2007,16(19):2272-2280.
[11]Tanaka H,Yamaba H,Kosugi N,Mizutani H,Nakata S.:Fermentable metabolite of Zymomonas mobilis controls collagen reduction in photoaging skin by improving TGF-beta/Smad signaling suppression.[J].Arch.Dermatol.Res.,2008,300(Suppl 1):S57-S64.
[12]Rannug A,Rannug U,Rosenkranz HS,Winqvist L,Westerholm R,Agurell E,Grafstrom AK.:Certain photooxidized derivatives of tryptophan bind with very high affinity to the Ah receptor and are likely to be endogenous signal substances.[J].J.Biol.Chem.,1987,262(32):15422-15427.
[13]Oberg M,Bergander L,Hakansson H,Rannug U,Rannug A.:Identification of the tryptophan photoproduct 6-formylindolo[3,2-b]carbazole,in cell culture medium,as a factor that controls the background aryl hydrocarbon receptor activity.[J].Toxicol.Sci.,2005,85(2):935-943.
[14]Wei YD,Rannug U,Rannug A.:UV-induced CYP1 Al gene expression in human cells is mediated by tryptophan.[J].Chem.Biol.Interact.,1999,118(2):127-140.
[15]Fritsche E,Schafer C,Calles C,Bernsmann T,Bernshausen T,Wurm M,Hubenthal U,Cline JE,Hajimiragha H,Schroeder P,Klotz LO,Rannug A,F(u|¨)rst P,Hanenberg H,Abel J,Krutmann J.:Lightening up the UV response by identification of the arylhydrocarbon receptor as a cytoplasmatic target for ultraviolet B radiation.[J].Proc.Natl.Acad.Sci.U.S.A.,2007,104(21):8851-8856.
[16]Kaestner KH,Knochel W,Martinez DE.:Unified nomenclature for the winged helix/forkhead transcription factors.[J].Genes.Dev.,2000,14(2):142-146.
[17]Carlsson P,Mahlapuu M.:Forkhead transcription factors:key players in development and metabolism.[J].Dev.Biol.,2002,250(1):1-23.
[18]Accili D,Arden KC.FoxOs at the crossroads of cellular metabolism,differentiation,and transformation.[J].Cell,2004,117(4):421-426.
[19]Kurosu H,Yamamoto M,Clark JD,Pastor JV,Nandi A,Gumani P,McGuinness OP,Chikuda H,Yamaguchi M,Kawaguchi H,Shimomura I,Takayama Y,Herz J,Kahn CR,Rosenblatt KP,Kuro-o M.:Suppression of aging in mice by the hormone Klotho.[J].Science,2005,309(5742):1829-1833.
[20]福里隆一,後藤元信:実用超臨界流体技術.[M].日本:分離技術会,2012.