关于木瓜酵素在中等浓度阴离子表面活性剂体系中稳定性的研究
详细信息 查看官网全文
- 英文论文题名：A Study on the Stabilization of the Papain Enzyme in the Moderately Concentrated Anionic Surfactant System
- 论文作者：金志映 ; 金容震 ; 李钟硕 ; 范霄锋 ; 李海光 ; 延在昊
- 英文论文作者：Ji-Yeong Kim ; Yong-Jin Kim ; Jong-Suk Lee ; Xiaofeng Fan ; Hae-Kwang Lee ; Jae-Ho Yeon ; Cosmetics & Personal Care Research Institute ; Amore Pacific Corporation R&D Center ; AMOREPACIFIC(SHANGHAI) R&I Center Co. ; Ltd
- 年：2016
- 作者机构：爱茉莉太平洋技术研究院化妆品研究所;爱茉莉太平洋(上海)研发有限公司护肤产品研究所;
- 论文关键词：木瓜酵素 ; 微胶囊 ; 离子复合物 ; 多层胶囊 ; 阴离子表面活性剂
- 英文论文关键词：papain enzyme ; microcapsule ; ion complex ; multi-layer capsule ; anionic surfactant
- 会议召开时间：2016-06-15
- 会议录名称：第十一届中国化妆品学术研讨会论文集
- 英文会议录名称：The Proceedings of the 11th Seminar on Cosmetic China
- 语种：中文
- 分类号：TQ658;TQ925
- 学会代码：ZGXE
- 会议名称：第十一届中国化妆品学术研讨会
- 会议地点：中国上海
- 主办单位：中国香料香精化妆品工业协会
- 学会名称：中国香料香精化妆品工业协会
- 页数：5
- 文件大小：599k
- 原文格式：O
- 会议级别：全国
摘要
在中等浓度的阴离子表面活性剂体系,一些特殊的包装方式能够庇护木瓜酵素免受蛋白水解的攻击。目前,酵素的稳定性已经成为研究的主要问题。首次利用多元醇,聚乙二醇(PEG),聚丙二醇(PPG)和PEG-PPG-PEG嵌段共聚物将木瓜酵素稳定于微胶囊中。介绍了包含离子复合物的多层包裹方法。在中等浓度阴离子表面活性剂体系作为水洗型清洁剂中,表面活性剂和水能使酵素胶囊的表面膨胀,进入核心以至于木瓜酵素的效果不能有效发挥。在这种情况下,由月桂酰肌氨酸钠和聚季铵盐-6组成的离子复合物多层胶囊能够有效地阻止水渗入酵素核心。进行了活体测试和角质层更新速度评估。
Even in the moderately concentrated anionic surfactant system,some special encapsulation method can shield the papain enzyme from proteolytic attacks.The stabilization of enzyme has been a major issue for successful therapies.It was the first time stabilized an enzyme,papain in microcapsules by using polyols,polyethyleneglycol(PEG),polypropyleneglycol(PPG),and PEG-PPG-PEG block copolymer.Furthermore,multi-layer capsulation methods containing ion complex had been introduced.Such a moderately concentrated anionic surfactant system as wash-off cleansers,surfactants and water in the final products swell the surface of enzyme capsules and penetrate into the core so easily that the effect of papain enzyme can't be achieved.In this case,the ion complex multi-layer capsule composed of sodium lauroyl sarcosinate and polyquaternium-6 could effectively prevent water from penetration into the core enzyme,followed by in-vivo test,and evaluate the stratum corneum(SC) turn-over speeds.
Even in the moderately concentrated anionic surfactant system,some special encapsulation method can shield the papain enzyme from proteolytic attacks.The stabilization of enzyme has been a major issue for successful therapies.It was the first time stabilized an enzyme,papain in microcapsules by using polyols,polyethyleneglycol(PEG),polypropyleneglycol(PPG),and PEG-PPG-PEG block copolymer.Furthermore,multi-layer capsulation methods containing ion complex had been introduced.Such a moderately concentrated anionic surfactant system as wash-off cleansers,surfactants and water in the final products swell the surface of enzyme capsules and penetrate into the core so easily that the effect of papain enzyme can't be achieved.In this case,the ion complex multi-layer capsule composed of sodium lauroyl sarcosinate and polyquaternium-6 could effectively prevent water from penetration into the core enzyme,followed by in-vivo test,and evaluate the stratum corneum(SC) turn-over speeds.
引文
[1]Wang J,Ibanez A,Chatrathi M P.On-chip integration of enzyme and immunoassays:simultaneous measurements of insulin and glucose[J].Journal of the American Chemical Society,2003,125(28):e424-e428.
[2]Besteman K,Lee J O,Wiertz F G M,etc.Enzyme-Coated Carbon Nanotubes as Single-Molecule Biosensors[J].Nano Letters,2003,3(6):727-730.
[3]Pei J,Tian F,Thundat T.Glucose biosensor based on the microcantilever[J].Analytical Chemistry,2004,76(2):292-296.
[4]Gomes F M,Pereira E B,Castro H F D.Immobilization of lipase on chitin and its use in nonconventional biocatalysis[J].Biomacromolecules,2004,5(1):17-23.
[5]Kim J W,Jung M O,Kim Y J,etc.Stabilization of Enzyme by Exclusive Volume Effect in Hydrophobically Controlled Polymer Microcapsules[J].Macromolecular Rapid Communications,2005,26(15):1258-1261.
[6]Ju H K.AMOPREPACIFIC CORPORATION.Method of stabilization of active ingredients using polyol/polymer microcapsules and cosmetic composition containing the microcapsules[p].US:20040108608,2004.
[7]Weichelman K,Braun R,Fitzpatrick J.Investigation of the bicinchoninic acid protein assay:identification of the groups responsible for color formation[J].Analytical Biochemistry,1988,175(1):231-237.
[2]Besteman K,Lee J O,Wiertz F G M,etc.Enzyme-Coated Carbon Nanotubes as Single-Molecule Biosensors[J].Nano Letters,2003,3(6):727-730.
[3]Pei J,Tian F,Thundat T.Glucose biosensor based on the microcantilever[J].Analytical Chemistry,2004,76(2):292-296.
[4]Gomes F M,Pereira E B,Castro H F D.Immobilization of lipase on chitin and its use in nonconventional biocatalysis[J].Biomacromolecules,2004,5(1):17-23.
[5]Kim J W,Jung M O,Kim Y J,etc.Stabilization of Enzyme by Exclusive Volume Effect in Hydrophobically Controlled Polymer Microcapsules[J].Macromolecular Rapid Communications,2005,26(15):1258-1261.
[6]Ju H K.AMOPREPACIFIC CORPORATION.Method of stabilization of active ingredients using polyol/polymer microcapsules and cosmetic composition containing the microcapsules[p].US:20040108608,2004.
[7]Weichelman K,Braun R,Fitzpatrick J.Investigation of the bicinchoninic acid protein assay:identification of the groups responsible for color formation[J].Analytical Biochemistry,1988,175(1):231-237.