中子管中子对小鼠宫颈癌肺转移治疗作用的初步研究
详细信息 查看官网全文
- 英文论文题名：The therapeutic effect of fast neutron from neutron tube on cervical cancer pulmonary metastasis mice:an in vivo study
- 论文作者：李环宇 ; 景士伟
- 英文论文作者：LI Huang-yu ; JING Shi-wei
- 年：2015
- 作者机构：东北师范大学物理学院;中国原子能研究院核技术应用研究所;
- 论文关键词：快中子 ; 肺转移模型 ; 生存分析
- 英文论文关键词：fast neutron ; pulmonary metastasis model ; survival analysis
- 会议召开时间：2015-09-21
- 会议录名称：中国核科学技术进展报告（第四卷）——中国核学会2015年学术年会论文集第8册（同位素分卷、辐射研究与应用分卷、核技术工业应用分卷、核农学分卷、核医学分卷、核情报分卷）
- 语种：中文
- 分类号：R730.55;R737.33
- 学会代码：EGVD
- 会议名称：中国核学会2015年学术年会
- 会议地点：中国四川绵阳
- 主办单位：中国核学会
- 学会名称：中国核学会
- 页数：6
- 文件大小：528k
- 原文格式：O
- 会议级别：全国
摘要
目的:探讨不同剂量快中子对肺转移模型小鼠的作用及其对荷瘤鼠生存期的影响,为中子治疗肿瘤的临床应用提供实验依据。方法:应用小鼠宫颈癌U14细胞株尾静脉注射法构建小鼠宫颈癌体内肺转移模型;采用不同剂量的快中子对荷瘤鼠全身照射,于照射后第10 d各组随机处死5只实验鼠,HE染色检测肺转移结节大小与数量;其余荷瘤鼠继续常规饲养,直至荷瘤鼠全部死亡,Kaplan-Meier统计方法进行生存期分析。结果:解剖学检测与HE染色均证实U14肺转移模型制作成功。与对照组比较,快中子照射30 min和60 min实验组小鼠肺转移癌结节数减少,荷瘤鼠的生存期延长,90 min实验组小鼠的肺转移癌结节数更少但荷瘤鼠的生存期缩短。结论:适当选择快中子照射剂量可有效抑制癌细胞肺转移,提高荷瘤鼠生存期。
Objective To study the biological effective and life-span of fast neutron on tumor-bearing mice to provide the theoretical basis for further clinical practice.Methods The mice models of pulmonary metastasis by murine cervical cancer were established by U14 cells injection viatail vein,and then the models were divided into four groups at random.On 10 th day after fast neutron radiotherapy,5 mice in each group were sacrificed for the evaluation of tumor inhibition efficacy and the other pulmonary metastasis models were raised and the death time of each model was recorded for Kaplan-Meier survival analysis.Results The mice models were successfully established which were confirmed by anatomy and HE stain assay.The results indicated that the fast neutron inhibited the growth and pulmonary metastasis of U14 in vivo.The mice models treated by fast neutron for 30 min and 60 min gained longer life-span and less metastatic nodules in lung tissues,whereas the mice in 90 min group gained shorter life-span and the least metastatic nodules,compared with the control group,respectively.Conclusions The pulmonary metastasis of U14 were inhibited by fast neutron in a dose-dependent manner,and a proper dosage should be selected for the radiotherapy of tumorbearing mice.
Objective To study the biological effective and life-span of fast neutron on tumor-bearing mice to provide the theoretical basis for further clinical practice.Methods The mice models of pulmonary metastasis by murine cervical cancer were established by U14 cells injection viatail vein,and then the models were divided into four groups at random.On 10 th day after fast neutron radiotherapy,5 mice in each group were sacrificed for the evaluation of tumor inhibition efficacy and the other pulmonary metastasis models were raised and the death time of each model was recorded for Kaplan-Meier survival analysis.Results The mice models were successfully established which were confirmed by anatomy and HE stain assay.The results indicated that the fast neutron inhibited the growth and pulmonary metastasis of U14 in vivo.The mice models treated by fast neutron for 30 min and 60 min gained longer life-span and less metastatic nodules in lung tissues,whereas the mice in 90 min group gained shorter life-span and the least metastatic nodules,compared with the control group,respectively.Conclusions The pulmonary metastasis of U14 were inhibited by fast neutron in a dose-dependent manner,and a proper dosage should be selected for the radiotherapy of tumorbearing mice.
引文
[1]段志凯,郭万龙,刘建功.中子辐射生物效应研究进展[J].辐射防护通讯,2008,28(5):19-22.
[2]肖雪夫,容超凡.关于中子在放射医学领域应用的思考[J].中华放射医学与防护杂志,2010,30(2):109-110.
[3]Douglas JG,Koh WJ,Austin-Seymour M,et al.Treatment of salivary gland neoplasms with fast neutron radiotherapy.Arch Otolaryngol Head Neck Surg.2003,129(9):944-8.
[4]Douglas JG,Goodkin R,Laramore GE.Gamma knife stereotactic radiosurgery for salivary gland neoplasms with base of skull invasion following neutron radiotherapy[J].Head Neck,2008,30(4):492-6.
[5]Bittner N,Koh WJ,Laramore GE,et al.Treatment of locally advanced adenoid cystic carcinoma of the trachea with neutron radiotherapy[J].Int J Radiat Oncol Biol Phys.2008,72(2):410-4.
[6]孙婷,周幽心.多形性胶质母细胞瘤的硼中子俘获治疗研究进展[J].中国神经肿瘤杂志,2010,8(3):208-211.
[7]杨杰,波拉提,阿依古丽,等.外照射结合~(252)Cf(~(252)Cf)中子近距离治疗恶性肿瘤近期疗效[J].实用肿瘤学杂志,2006,20(85):428-429.
[8]郑秀惠,陈竹钦,李力,等.宫颈鳞癌中子放疗前后细胞凋亡与PCNA(增殖细胞核抗原)的表达变化[J].中国妇幼保健,2009,21(6):826-828.
[9]张玉晶,刘新帆,冯勤付,等.快中子放射治疗软组织肉瘤的疗效分析[J].中华放射肿瘤学杂志,2003,12(3):165-169
[10]Kawabata S,Miyatake S,Nonoguchi N,et al.Survival benefit from boron neutron capture therapy for the newly diagnosed glioblastoma patients[J].Appl Radiat Isot,2009,67(7-8):15-18.
[2]肖雪夫,容超凡.关于中子在放射医学领域应用的思考[J].中华放射医学与防护杂志,2010,30(2):109-110.
[3]Douglas JG,Koh WJ,Austin-Seymour M,et al.Treatment of salivary gland neoplasms with fast neutron radiotherapy.Arch Otolaryngol Head Neck Surg.2003,129(9):944-8.
[4]Douglas JG,Goodkin R,Laramore GE.Gamma knife stereotactic radiosurgery for salivary gland neoplasms with base of skull invasion following neutron radiotherapy[J].Head Neck,2008,30(4):492-6.
[5]Bittner N,Koh WJ,Laramore GE,et al.Treatment of locally advanced adenoid cystic carcinoma of the trachea with neutron radiotherapy[J].Int J Radiat Oncol Biol Phys.2008,72(2):410-4.
[6]孙婷,周幽心.多形性胶质母细胞瘤的硼中子俘获治疗研究进展[J].中国神经肿瘤杂志,2010,8(3):208-211.
[7]杨杰,波拉提,阿依古丽,等.外照射结合~(252)Cf(~(252)Cf)中子近距离治疗恶性肿瘤近期疗效[J].实用肿瘤学杂志,2006,20(85):428-429.
[8]郑秀惠,陈竹钦,李力,等.宫颈鳞癌中子放疗前后细胞凋亡与PCNA(增殖细胞核抗原)的表达变化[J].中国妇幼保健,2009,21(6):826-828.
[9]张玉晶,刘新帆,冯勤付,等.快中子放射治疗软组织肉瘤的疗效分析[J].中华放射肿瘤学杂志,2003,12(3):165-169
[10]Kawabata S,Miyatake S,Nonoguchi N,et al.Survival benefit from boron neutron capture therapy for the newly diagnosed glioblastoma patients[J].Appl Radiat Isot,2009,67(7-8):15-18.