壳聚糖/累托石层层自组装丝素纳米纤维膜的抑菌及生物相容性研究
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- 英文论文题名：Antibacterial Activity and Biocompatibility of Chitosan/Rectorite Layer-By-Layer Self-Assembled Silk Fibroin Nanofibrous Mats
- 论文作者：陈佳佳 ; 刘荣 ; 邓红兵 ; 施晓文 ; 杜予民
- 英文论文作者：Jiajia Chen ; Rong Liu ; Hongbing Deng ; Xiaowen Shi ; Yumin Du ; School of Resource and Environmental Science ; Wuhan University
- 年：2016
- 作者机构：武汉大学资源与环境科学学院;
- 论文关键词：静电纺丝 ; 壳聚糖 ; 累托石 ; 抑菌 ; 生物相容性
- 英文论文关键词：Electrospin ; Chitosan ; Rectorite ; Antibacterial activity ; Biocompatibility
- 会议召开时间：2016-11-18
- 会议录名称：中国第四届静电纺丝大会（CICE 2016）摘要集
- 语种：中文
- 分类号：TQ340.64
- 学会代码：ZGNM
- 会议名称：中国第四届静电纺丝大会(CICE 2016)
- 会议地点：中国北京
- 主办单位：中国复合材料学会超细纤维材料分会（筹）
- 学会名称：中关村科创纳米技术创新研究会
- 页数：1
- 文件大小：2501k
- 原文格式：D
- 会议级别：全国
摘要
丝素蛋白(SF)具有良好的生物相容性以及可控的生物降解性。通过静电纺丝法制备得到的丝素蛋白纳米纤维膜具有较大的孔隙率和与细胞外基质类似的独特的三维结构,使其在组织工程领域有较为广泛的应用[1]。本研究以丝素蛋白纳米纤维膜为底板,以带正电荷的壳聚糖(CS)和带负电荷的累托石(REC)作为组装成分,以静电力为驱动力,对丝素底板进行了层层自组装(LBL),并探究了该复合膜的抑菌性能以及其生物相容性。扫描电镜(SEM)测试结果表明,该复合膜经层层自组装后,其直径增大。通过傅里叶红外光谱(FT-IR)、拉曼散射光谱、光电子能谱(XPS)、X射线衍射(XRD)测试,证实了壳聚糖和累托石被成功组装在了丝素膜底板上。同时,抑菌试验结果表明,该复合膜可以抑制大肠杆菌和金黄色葡萄球菌的生长,对大肠杆菌的抑制率最高可达84%,对金黄色葡萄球菌的抑制率最高可达92%。细胞实验结果证明,壳聚糖和累托石的组装后的丝素膜仍具有较好的生物相容性,细胞可以在复合膜表面贴附、生长和增殖。
This work focused on the electrospun silk fibroin(SF) nanofibers which were layer-by-layer assembled by chitosan(CS) and rectorite(REC).The composites were characterized by SEM,FT-IR,Raman spectroscopy,XPS and XRD,respectively.These results indicated that the diameter of composite nanofibers increased,and the assembly of CS and REC was successful.The antibacterial activity assay showed that the introduction of CS and REC enhanced the antibacterial activity of the SF fibroin nanofibrous mats.The antibacterial activity of LBL self-assembled SF nanofibrous mats against E.coli and S.aureus was 84%and 92%,respectively.The cell culture experiments demonstrated that the mats still had good biocompatibility after the introduction of CS and REC.
This work focused on the electrospun silk fibroin(SF) nanofibers which were layer-by-layer assembled by chitosan(CS) and rectorite(REC).The composites were characterized by SEM,FT-IR,Raman spectroscopy,XPS and XRD,respectively.These results indicated that the diameter of composite nanofibers increased,and the assembly of CS and REC was successful.The antibacterial activity assay showed that the introduction of CS and REC enhanced the antibacterial activity of the SF fibroin nanofibrous mats.The antibacterial activity of LBL self-assembled SF nanofibrous mats against E.coli and S.aureus was 84%and 92%,respectively.The cell culture experiments demonstrated that the mats still had good biocompatibility after the introduction of CS and REC.
引文
[1]Melke,J.;Midha,S.;Ghosh,S.;et al.Acta.Biomater.2016,31:1.