南川百合种子萌发及试管鳞茎的培育初探
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- 英文论文题名：Study on Germination and Bulb Cultivation in Tube of Lilium rosthornii Diels
- 论文作者：丁瑞华 ; 李红梅 ; 赵玉倩 ; 贾桂霞
- 英文论文作者：DING Rui-hua ; LI Hong-mei ; ZHAO Yu-qian ; JIA Gui-xia ; Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding ; National Engineering Research Center for Floriculture ; Beijing Laboratory of Urban and Rural Ecological Environment and College of Landscape Architecture ; Beijing Forestry University ; Hunan Province Yuanling County Agricultural Technology Promotion Center
- 年：2016
- 作者机构：花卉种质创新与分子育种北京市重点实验室国家花卉工程技术研究中心城乡生态环境北京实验室园林学院北京林业大学;湖南省沅凌县农业技术推广中心;
- 论文关键词：南川百合 ; 试管鳞茎 ; 幼苗生长
- 英文论文关键词：L.rosthornii ; Bulblet in tube ; The growth of seedling
- 会议召开时间：2016-07-19
- 会议录名称：中国观赏园艺研究进展2016
- 语种：中文
- 分类号：S682.29
- 学会代码：EGYP
- 会议名称：2016年中国观赏园艺学术研讨会
- 会议地点：中国湖南长沙
- 主办单位：中国园艺学会观赏园艺专业委员会、国家花卉工程技术研究中心
- 学会名称：中国园艺学会
- 页数：6
- 文件大小：1392k
- 原文格式：O
- 会议级别：全国
摘要
南川百合为中国特有植物,本研究对组培条件下种子萌发和试管鳞茎增大的影响因素、以及光照和多效唑对移栽后幼苗生长的影响进行了探究,研究结果表明:①南川百合种子萌发与温度、剥种皮处理、基本培养基大量元素含量等因素相关,南川百合种子最适宜的萌发温度为15~20℃;剥种皮处理的种子具有最高的发芽率和发芽势,分别为90.48%、42.86%;使用1/2MS培养基的种子在第9天开始萌发,并且具有较高的发芽势和发芽率。②试管鳞茎的增大与蔗糖浓度及活性炭含量相关,蔗糖浓度为60g/L以及活性炭含量为1.00g/L的培养基能够显著加快试管鳞茎的增大。③施用0.5mg/L的多效唑能够促进南川百合幼苗叶片数量增加,提高其移栽成活率;长日照对于南川百合的幼苗生长没有影响。该研究对南川百合的繁殖和引种驯化具有一定的实际应用价值。
Lilium rosthornii is an endemic plant to China.It aims to study the tissue culture conditions which influenced seed germination,bulblet increasing and the growth of tissue culture seedling,to establish tube bulb cultivation and breeding technology of preliminary system of L.rosthornii under tissue culture conditions.It indicated that L.rosthornii seeds germination was related to temperature,seed coat,culture medium and many factors;the optimum seeds germination temperature was 15-20℃;peeling the coat of the seeds had the highest germination rate,germination potential 90.48%,42.86%;seeds began to germinate in the 9th day under 1/2 MS culture medium and had a higher germination potential and germination rate.The increase of tube bulb was related to the concentration of sucrose and the concentration of activated carbon.The sucrose concentration of 60g/L and the concentration of activated carbon for 1.00g/L could significantly accelerated the growth of tube bulb.The long day for the growth of L rosthornii was not significant,and the application of 0.5 mg/L paclobutrazol can promote the increasing in the number of L.rosthornii seedling leaves and improvement of the survival rate.
Lilium rosthornii is an endemic plant to China.It aims to study the tissue culture conditions which influenced seed germination,bulblet increasing and the growth of tissue culture seedling,to establish tube bulb cultivation and breeding technology of preliminary system of L.rosthornii under tissue culture conditions.It indicated that L.rosthornii seeds germination was related to temperature,seed coat,culture medium and many factors;the optimum seeds germination temperature was 15-20℃;peeling the coat of the seeds had the highest germination rate,germination potential 90.48%,42.86%;seeds began to germinate in the 9th day under 1/2 MS culture medium and had a higher germination potential and germination rate.The increase of tube bulb was related to the concentration of sucrose and the concentration of activated carbon.The sucrose concentration of 60g/L and the concentration of activated carbon for 1.00g/L could significantly accelerated the growth of tube bulb.The long day for the growth of L rosthornii was not significant,and the application of 0.5 mg/L paclobutrazol can promote the increasing in the number of L.rosthornii seedling leaves and improvement of the survival rate.
引文
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2.何林.2006.贮藏温度对南川百合花粉萌发率的影响[J].热带农业科技,13-14.
3.赵静,帅明蓉,赵玉飞,等.2012.南川百合组织培养与快繁技术体系研究[J].西南师范大学学报(自然科学版),109-115.
4.刘华敏.2010.部分野生百合(Lilium)的核型和ISSR分析[D]:西南大学博士论文.
5.张腾旬,孙明.2015.烯萜合酶基因的生物信息学分析[J].中国福建厦门,9.
6.刘燕琴,胡开治,肖波,等.2006.南川百合组织培养研究[J].中国现代中药,32-33.
7.王瑞波.2009.中国特有植物南川百合(Lilium rosthornii Diels)保护生物学研究[D]:西南大学博士论文.
8.孙晓玉,杨利平,姜浩野,等.2003.条叶百合种子萌发的研究[J].植物研究,61-65.
9.王爱勤,周歧伟,何龙飞,等.1998.百合试管结鳞茎的研究[J].广西农业大学学报,71-75.