黑曲霉JMU-TS528模拟固态发酵茶梗产单宁酶
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- 英文论文题名：Tannase productionby modified solid-state fermentation with tea stalks using aspergillus niger JMU-TS528
- 论文作者：张枫 ; 吴昌正 ; 肖安风
- 英文论文作者：Zhang Feng ; Wu Changzhegn ; Xiao Anfeng ; College of Food and Biological Engineering ; Jimei University ; Key Laboratory of Food Microbiology and Enzyme Engineering of Fujian Province ; Fujian Provincial Engineering Technology Research Center of marine functional food ; Xiamen Key Laboratory of Marine Functional Food
- 年：2016
- 作者机构：集美大学食品与生物工程学院;福建省食品微生物与酶工程重点实验室;福建省海洋功能食品工程技术研究中心;厦门市海洋功能食品重点实验室;
- 论文关键词：单宁酶 ; 黑曲霉 ; 茶梗 ; 模拟固态发酵 ; 响应面
- 英文论文关键词：tannase ; aspergillus niger ; tea stalks ; modified solid-state fermentation ; response surface methodology
- 会议召开时间：2016-11-09
- 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
- 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
- 语种：中文
- 分类号：TQ925
- 学会代码：ZGSP
- 会议名称：中国食品科学技术学会第十三届年会
- 会议地点：中国北京
- 主办单位：中国食品科学技术学会
- 学会名称：中国食品科学技术学会
- 页数：2
- 文件大小：113k
- 原文格式：O
- 会议级别：全国
摘要
本试验对黑曲霉JMU-TS528模拟固态发酵茶叶梗生产单宁酶的工艺条件进行优化。首先,通过单因素试验,选择出对生物量积累和单宁酶合成有显著促进作用的碳源和氮源物质,再通过响应面法优化所选择的组合碳氮源的添加量。单因素试验确定了最适的碳源组合为葡萄糖和单宁酸,最适氮源组合为硫酸铵和酵母浸膏。本试验以单宁酶活力为指标,利用响应面法优化碳氮源浓度,建立了单宁酸添加量、葡萄糖添加量、硫酸铵添加量、酵母浸膏添加量与单宁酶活力之间的数学模型。试验结果表明最佳发酵培养基条件为:单宁酸、葡萄糖、硫酸铵以及酵母浸膏的添加量分别为7.49%、8.11%、9.26%和2.25%,在此条件下,发酵120 h可获得最大单宁酶活力19.22 U/gds,是优化前最大酶活力的6.36倍。
In this study,a modified solid-state fermentationconditions were optimized for tannase production with tea stalksusing Aspergillus nigerJMU-TS528.Firstly,the optimal carbon source and nitrogen source were selected to improve biomass accumulation and enzyme synthesis.On the base of single-factor experiment,tannic acid and glucose were determined as the carbon sources.Meanwhile,ammonium sulfate and yeast extract were affirmed as the nitrogen sources.Then,the addition amount of combined carbon and nitrogen sources were chosen by response surface methodology.A mathematical regression model was established for predicting the activity of tannase at different levels of tannic acid addition,glucose addition,(NH_4)_2SO_4 addition,and yeast extract addition.The optimal culture conditions were determined to be 7.49%,8.11%,9.26%,2.25%for tannic acid,glucose,(NH_4)_2SO_4 and yeast extract addition,respectively.Under the optimum conditions,the activity of tannase was up to 19.22 U/gds after 120 h incubation,which was 6.36 times over the activity of initial conditions.
In this study,a modified solid-state fermentationconditions were optimized for tannase production with tea stalksusing Aspergillus nigerJMU-TS528.Firstly,the optimal carbon source and nitrogen source were selected to improve biomass accumulation and enzyme synthesis.On the base of single-factor experiment,tannic acid and glucose were determined as the carbon sources.Meanwhile,ammonium sulfate and yeast extract were affirmed as the nitrogen sources.Then,the addition amount of combined carbon and nitrogen sources were chosen by response surface methodology.A mathematical regression model was established for predicting the activity of tannase at different levels of tannic acid addition,glucose addition,(NH_4)_2SO_4 addition,and yeast extract addition.The optimal culture conditions were determined to be 7.49%,8.11%,9.26%,2.25%for tannic acid,glucose,(NH_4)_2SO_4 and yeast extract addition,respectively.Under the optimum conditions,the activity of tannase was up to 19.22 U/gds after 120 h incubation,which was 6.36 times over the activity of initial conditions.
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