不同C/N比反硝化脱氮及微生物群落结构对比分析
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摘要
本研究旨在分析Fe-C内电解载体处理不同C/N(COD/TN)比硝氮污染水体反硝化效率及微生物群落结构差异.结果显示:当C/N=0时,反应体系硝氮去除率达到100%,总氮去除率为92.0%.当C/N=1时,反应体系硝氮去除率为100%,总氮去除率为93.0%.体系中COD的存在会使反硝化作用在较短时间内达到稳定.C/N=0时系统中主要的反硝化细菌为自养反硝化细菌Thiobacillus,相对比例为30.0%,同时内电解反应产生的氨氮会导致Nitrosospira存在较高比例.C/N=1时反硝化体系中主要以兼性营养反硝化细菌Thauera为主,相对比例为61.6%.结果表明,不同C/N条件下反硝化效能无显著差异,但不同体系中反硝化功能细菌差异明显.
引文
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[5]Zhang X,Qu Y,Ma Q,et al.Illumina MiSeq sequencing reveals diverse microbial communities of activated sludge systems stimulated by different aromatics for indigo biosynthesis from indole[J].PloS one,2015,10(4):e0125732.
[6]Ren G,Zhang H,Lin X,et al.Response of phyllosphere bacterial communities to elevated C02 during rice growing season[J].Applied microbiology and biotechnology,2014,98(22):9459-9471.
[7]Saarenheimo J,Tiirola M A,Rissanen A J.Functional gene pyrosequencing reveals core proteobacterial denitrifiers in boreal lakes[J].Frontiers in microbiology,2015,6.
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[9]Mao Y,Xia Y,Zhang T.Characterization of Thauera-dominated hydrogen-oxidizing autotrophic denitrifying microbial communities by using high-throughput sequencing[J].Bioresource technology,2013,128:703-710.