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盐度凡纳滨对虾饲料中适宜蛋白质水平的研究
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摘要
为研究低盐度凡纳滨对虾饲料中最适蛋白质水平,采用蛋白质水平分别为25%、30%、35%、40%和45%的5种等能不等氮饲料投喂盐度2的凡纳滨对虾仔稚虾(平均体重0.31±0.02g)56d。结果表明:(1)随着饲料蛋白质水平升高,凡纳滨对虾末重、增重率和特定生长率呈先升后降趋势,35%蛋白组显著高于其他各组(p<0.05)。通过对饲料蛋白质水平和增重率、特定生长率的二次回归分析表明,饲料蛋白质含量在33.51%~34.35%时,凡纳滨对虾有最大的增重率的生长效果。(2)随饲料蛋白质水平的升高,虾体水分含量呈基本下降趋势,虾体蛋白含量呈上升趋势,虾体脂肪和灰分含量无显著性变化。(3)随着蛋白质水平升高,蛋白酶的含量呈现先升高后降低的趋势,35%组活性最高,显著高于其他各组(p<0.05),而脂肪酶和淀粉酶的含量无显著性差异。
In order to find the optimal dietary protein level for Litopenaeus vannamei in low salinity water, isolipid and isocaloric diets with different protein levels(25%, 30%, 35%, 40% and45%) were used to feed L. vannamei juveniles(mean weight 0.31 ± 0.02 g) for 56 days in salinity2 practical salinity unit(psu) water. The results showed that:(1) As the dietary protein level increased, the final body weight, weight gain and specific growth rate increased at first and then decreased. In the 35% protein level group, significantly better results were obtained as compared to other groups(p < 0.05), Through quadratic regression analysis of dietary protein level and weight gain and specific growth rate, we found that shrimps had the highest weight gain when dietary protein level was between 33.51%–34.35%;(2) As the dietary protein level increased, the shrimp water content decreased and protein content increased while lipid and ash content did not significantly change;(3) As the dietary protein level increased, the activity of trypsin increased at first and then decreased. The 35% protein level group had the highest trypsin activity, significantly higher than other groups(p < 0.05), there were no significant differences in the activities of lipase or amylase.
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