柱前衍生化-高效液相色谱分离食用油中的甘油三酯
详细信息 查看官网全文
- 英文论文题名：Separation of triacylglycerols in edible oil by high performance liquid chromatography using pre-column derivatization of bromine
- 论文作者：许旭 ; 山晓琳 ; 刘晓婷 ; 龚灿 ; 雷小瑞 ; 赵海燕
- 英文论文作者：Xu Xu ; Xiao-Lin Shan ; Xiao-Ting Liu ; Can Gong ; Xiao-Rui Lei ; Hai-Yan Zhao ; School of Chemical and Environmental Engineering ; Shanghai Institute of Technology
- 年：2016
- 作者机构：上海应用技术大学化学与环境工程学院;
- 论文关键词：食用油 ; 甘油三酯 ; 衍生化 ; 高效液相色谱
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第二十三分会：复杂样品分离分析
- 语种：中文
- 分类号：O652.63;TS227
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第二十三分会 ; 复杂样品分离分析
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：140k
- 原文格式：D
- 会议级别：全国
摘要
甘油三酯(TAG)是食用油的主要成分。可以根据甘油三酯的组成来识别不同的食用油及食用油掺假。目前用于识别和分析食用油的方法以HPLC与LC-MS应用较多。TG的衍生化通常用于研究双键位置,臭氧化反应是较早用于脂肪酸和甘油三酯分析的衍生化反应。用于脂肪酸和甘油三酯分析的衍生化反应还有复分解反应、光环化反应、和Diels-Alder反应等。我们以溴作为烯键加成衍生化试剂,可以改善HPLC分离甘油三酯的选择性。采用溴的四氯化碳溶液作为衍生化试剂,产物用非水反相高效液相色谱法进行分离分析。在简单的避光恒温衍生化反应条件下,全部双键被溴加成,加成反应产物可以通过非水反相HPLC分离出更多的色谱峰,显示该法可显著改善TAG的HPLC分离选择性。为食用油中甘油三酯的分离分析提供了新思路。
The complexity of triacylglycerols(TAGs) in edible oils is largely due to the many similar unsaturated TAGs composition,which made the TAGs profiling quite difficult.In this study,pre-column derivatization with bromine(Br_2) was used to improve the separation of TAGs in edible oil by HPLC.Oil sample dissolved in n-hexane was derived by reaction with Br_2-CCl_4 solution at room temperature.The derivate sample solution can be separated better by HPLC using a C18 column and mobile phase as methanol-hexane.The method was applied to profile 20 kinds of edible oil and the result presents difference in TAGs in various edible oils,which offers an alternative method for identification of edible oils.
The complexity of triacylglycerols(TAGs) in edible oils is largely due to the many similar unsaturated TAGs composition,which made the TAGs profiling quite difficult.In this study,pre-column derivatization with bromine(Br_2) was used to improve the separation of TAGs in edible oil by HPLC.Oil sample dissolved in n-hexane was derived by reaction with Br_2-CCl_4 solution at room temperature.The derivate sample solution can be separated better by HPLC using a C18 column and mobile phase as methanol-hexane.The method was applied to profile 20 kinds of edible oil and the result presents difference in TAGs in various edible oils,which offers an alternative method for identification of edible oils.
引文