变异黄芪自然中毒山羊病理学研究
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- 英文论文题名：Pathological Research on the Spontaneous Astrugalus variabilis Intoxication of Goats
- 论文作者：王姗姗 ; 路浩 ; 周宏超 ; 达能太 ; 王文龙 ; 赵宝玉
- 英文论文作者：WANG Shan-shan ; LU Hao ; ZHOU Hong-chao ; Da Neng-tai ; WANG Wen-long ; ZHAO Bao-yu ; College of Veterinary Medicine ; Northwest A&F University ; Alasan Left Banner Veterinary Station of Inner Mongolia
- 年：2014
- 作者机构：西北农林科技大学动物医学院;内蒙古阿拉善左旗兽医工作站;
- 论文关键词：内蒙古 ; 疯草 ; 变异黄芪 ; 苦马豆素 ; 山羊 ; 病理学
- 英文论文关键词：Inner Mongolia ; Locoweeds ; Astrugalus variabilis ; Swainsonine ; Goats ; Pathology
- 会议召开时间：2014-11-29
- 会议录名称：陕西省毒理学会2014年学术会议论文集
- 语种：中文
- 分类号：S858.27
- 学会代码：XBST
- 会议名称：陕西省毒理学会2014年学术会议
- 会议地点：中国陕西西安
- 主办单位：陕西省毒理学会
- 学会名称：西北大学生态毒理研究所
- 页数：8
- 文件大小：4744k
- 原文格式：O
- 会议级别：地方
摘要
变异黄芪是一种疯草类有毒植物,其毒性成分为苦马豆素,本实验以内蒙古阿拉善左旗吉兰泰镇的两只变异黄芪自然中毒山羊为研究对象,通过HE染色观察脑、心、肝、脾、肾等组织病理学变化,透射电镜观察脑、心、肝组织超微结构变化。结果发现,中毒羊脑、心、肝、肾等组织细胞发生广泛空泡变性,超微结构观察发现大小脑、心、肝细胞中有多量膜包裹的空泡样结构,血管中有炎性细胞浸润,线粒体嵴断裂,排列紊乱、变性。中毒羊的病理变化与超微结构变化与之前报道的疯草中毒动物的基本一致,但线粒体变性则报道的较少。
Astrugalus variabilis is a species of locoweeds,whose main toxic ingredient is swainsonine.We found two goats intoxicated by Astrugalus variabilis spontaneously in Jilantai,Alxa,Inner Mogolia,the tissues of the goats were collected to go through the histology process,histology lesions of cerebrum,cerebellum,heart,liver were studied through HE stain,and the ultrastructure changes were studied using transmission electron microscope.Results show that the most prominent lesion was cytoplasmic vacuolation in neurons,Purkinje cells,hepatocytes,renal tubular epithelial cells,etc.Ultrastructurally,there were many vacuoles surrounded by single layer membranes in the cytoplasm,and there were inflammatory cells in the blood vessels.Mitochondria swelled irregularly and their cristae fractured and become fuzzy.The pathological results are almost consistant with that described in the related articles,but there are few articles mention the mitochondria lesions.
Astrugalus variabilis is a species of locoweeds,whose main toxic ingredient is swainsonine.We found two goats intoxicated by Astrugalus variabilis spontaneously in Jilantai,Alxa,Inner Mogolia,the tissues of the goats were collected to go through the histology process,histology lesions of cerebrum,cerebellum,heart,liver were studied through HE stain,and the ultrastructure changes were studied using transmission electron microscope.Results show that the most prominent lesion was cytoplasmic vacuolation in neurons,Purkinje cells,hepatocytes,renal tubular epithelial cells,etc.Ultrastructurally,there were many vacuoles surrounded by single layer membranes in the cytoplasm,and there were inflammatory cells in the blood vessels.Mitochondria swelled irregularly and their cristae fractured and become fuzzy.The pathological results are almost consistant with that described in the related articles,but there are few articles mention the mitochondria lesions.
引文
[1]周启武,赵宝玉,路浩,王姗姗,张樑,温伟利,杨晓雯.中国西部天然草地疯草生态及动物疯草中毒研究与防控现状[J].中国农业科学,2013,46(6):1280-1296.
[2]樊泽峰,王建军,赵宝玉,董强,达能太.内蒙古阿拉善盟草原疯草危害调查[J].中国草地学报,2006,28(2):56-59.
[3]董强,赵宝玉,童德文,葛鹏斌,哈斯巴图,达能太,邓珊丹,梁洁莉.阿拉善左旗天然草地毒草调查[J]_草业科学,2003,20(6):50-53.
[4]包根晓,杨建平,张永革,德力格尔,阿拉腾陶布其.阿拉善左旗北部天然草原遭有毒植物变异黄芪危害的成因及其防治对策[J].内蒙古草业,2005,17(1):39-49.
[5]黄有德,肖志国,孟聚诚,孔繁矼.变异黄芪有毒成分的分离与分析[J].兽医医药杂志,1992,4:3-6.
[6]王生奎,安清聪,王旭东.绵羊实验性变异黄芪和黄花棘豆中毒的比较[J].云南农业大学学报,1998,13(2):219-223.
[7]姚静雯,冯欢,袁玉川,尉亚辉,黄萱.草原毒草变异黄芪的研究进展[J].中国草地学报,2013,35(3):110-115.
[8]Armien AG,Tokarnia CH,Peixoto PV.Spontaneous and experimental glycoprotein storage disease of goats induced by Ipomoea carnea subsp.fistulosa(Convolvulaceae)[J].Vet Pathol,2007,44:170-184.
[9]Colegate SM,Dorling PR,Huxtable CR.A spectroscopic investigation of swainsonine:An alfa-mannosidase inhibitor isolated from Swainsona canescens[J].Aust J Chem,1979,32:2257-2264.
[10]Molyneux RJ,James LF.Loco intoxication:Indolizidine alkaloids of spotted locoweed(Astragalus lentiginosus)[J].SCIENCE,1982,216:190-191.
[11]Hueza IM,Dagli ML,Gorniak SL,Paulino CA.Toxic effect of prenatal Ipomoea carnea administration to rats[J].Vet.Hum.Toxicol.2003,45:298-302.
[12]刘宗平.动物中毒病学[M].北京:中国农业出版社,2006.
[13]Driemeier D,Colodel EM,Gimeno EJ,Barros SS.Lysosomal Storage Disease Caused by Sida carpinifolia Poisoning in Goats[J].Vet Pathol,2000,37:153-159.
[14]Armie'N AG,Tokarnia CH,Peixoto PV,Frese K.Spontaneous and Experimental Glycoprotein Storage Disease of Goats Induced by Ipomoea carnea subsp fistulosa(Convolvulaceae)[J].Vet Pathol,2007,44:170-184.
[15]Barbosa RC,Riet-Correa F,Medeirosb RMT,Lima EF,Barros SS,Gimeno EJ,Molyneux RJ,Gardner DR.Intoxication by Ipomoea sericophylla and Ipomoea riedelii in goats in the state of Parai'ba,Northeastern Brazil[J].TOXICON,2006,47:371-379.
[16]朱长庚.神经解剖学(第二版)[M].北京:人民卫生出版社,2009:473-556.
[17]Elvio ER,Luciana AC,Eduardo JG,Maria GG,Ofelia CAP.Experimental poisoning of goats by Ipomoea carnea subsp.fistulosa in Argentina:A clinic and pathological correlation with special consideration on the central nervous system[J].Pesq Vet Bras,2012,32(1):37-42.
[18]肖志国,王生奎,黄有德,陈怀涛,黄莉,王秋弹,朱秀琴,叶得河.绵羊实验性变异黄芪中毒[J].草与畜杂志,1994,1:7-9.
[19]Li Z,Huang Y,Dong F,Li W,Ding L,Yu G,Xu D,Yang Y,Xu X,Tong D.Swainsonine promotes apoptosis in human oesophageal squamous cell carcinoma cells in vitro and in vivo through activation of mitochondrial pathway.J Biosci,2012,37(6):1005-1016.
[2]樊泽峰,王建军,赵宝玉,董强,达能太.内蒙古阿拉善盟草原疯草危害调查[J].中国草地学报,2006,28(2):56-59.
[3]董强,赵宝玉,童德文,葛鹏斌,哈斯巴图,达能太,邓珊丹,梁洁莉.阿拉善左旗天然草地毒草调查[J]_草业科学,2003,20(6):50-53.
[4]包根晓,杨建平,张永革,德力格尔,阿拉腾陶布其.阿拉善左旗北部天然草原遭有毒植物变异黄芪危害的成因及其防治对策[J].内蒙古草业,2005,17(1):39-49.
[5]黄有德,肖志国,孟聚诚,孔繁矼.变异黄芪有毒成分的分离与分析[J].兽医医药杂志,1992,4:3-6.
[6]王生奎,安清聪,王旭东.绵羊实验性变异黄芪和黄花棘豆中毒的比较[J].云南农业大学学报,1998,13(2):219-223.
[7]姚静雯,冯欢,袁玉川,尉亚辉,黄萱.草原毒草变异黄芪的研究进展[J].中国草地学报,2013,35(3):110-115.
[8]Armien AG,Tokarnia CH,Peixoto PV.Spontaneous and experimental glycoprotein storage disease of goats induced by Ipomoea carnea subsp.fistulosa(Convolvulaceae)[J].Vet Pathol,2007,44:170-184.
[9]Colegate SM,Dorling PR,Huxtable CR.A spectroscopic investigation of swainsonine:An alfa-mannosidase inhibitor isolated from Swainsona canescens[J].Aust J Chem,1979,32:2257-2264.
[10]Molyneux RJ,James LF.Loco intoxication:Indolizidine alkaloids of spotted locoweed(Astragalus lentiginosus)[J].SCIENCE,1982,216:190-191.
[11]Hueza IM,Dagli ML,Gorniak SL,Paulino CA.Toxic effect of prenatal Ipomoea carnea administration to rats[J].Vet.Hum.Toxicol.2003,45:298-302.
[12]刘宗平.动物中毒病学[M].北京:中国农业出版社,2006.
[13]Driemeier D,Colodel EM,Gimeno EJ,Barros SS.Lysosomal Storage Disease Caused by Sida carpinifolia Poisoning in Goats[J].Vet Pathol,2000,37:153-159.
[14]Armie'N AG,Tokarnia CH,Peixoto PV,Frese K.Spontaneous and Experimental Glycoprotein Storage Disease of Goats Induced by Ipomoea carnea subsp fistulosa(Convolvulaceae)[J].Vet Pathol,2007,44:170-184.
[15]Barbosa RC,Riet-Correa F,Medeirosb RMT,Lima EF,Barros SS,Gimeno EJ,Molyneux RJ,Gardner DR.Intoxication by Ipomoea sericophylla and Ipomoea riedelii in goats in the state of Parai'ba,Northeastern Brazil[J].TOXICON,2006,47:371-379.
[16]朱长庚.神经解剖学(第二版)[M].北京:人民卫生出版社,2009:473-556.
[17]Elvio ER,Luciana AC,Eduardo JG,Maria GG,Ofelia CAP.Experimental poisoning of goats by Ipomoea carnea subsp.fistulosa in Argentina:A clinic and pathological correlation with special consideration on the central nervous system[J].Pesq Vet Bras,2012,32(1):37-42.
[18]肖志国,王生奎,黄有德,陈怀涛,黄莉,王秋弹,朱秀琴,叶得河.绵羊实验性变异黄芪中毒[J].草与畜杂志,1994,1:7-9.
[19]Li Z,Huang Y,Dong F,Li W,Ding L,Yu G,Xu D,Yang Y,Xu X,Tong D.Swainsonine promotes apoptosis in human oesophageal squamous cell carcinoma cells in vitro and in vivo through activation of mitochondrial pathway.J Biosci,2012,37(6):1005-1016.