长寿花花器官离体培养研究
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- 英文论文题名：Research on Flower Organ Tissue Culture of Kalanchoe blossfeldiana
- 论文作者：吕侃俐 ; 李青
- 英文论文作者：L Kan-li ; LI Qing ; Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding ; National Engineering Research Center for Floriculture ; Beijing Laboratory of Urban and Rural Ecological Environment and College of Landscape Architecture ; Beijing Forestry University
- 年：2016
- 作者机构：花卉种质创新与分子育种北京市重点实验室国家花卉工程技术研究中心城乡生态环境北京实验室园林学院北京林业大学;
- 论文关键词：长寿花 ; 花器官 ; 离体培养 ; 花芽诱导
- 英文论文关键词：Kalanchoe blossfeldiana ; Floral organ ; Tissue culture ; Floral bud induction
- 会议召开时间：2016-07-19
- 会议录名称：中国观赏园艺研究进展2016
- 语种：中文
- 分类号：S682.19
- 学会代码：EGYP
- 会议名称：2016年中国观赏园艺学术研讨会
- 会议地点：中国湖南长沙
- 主办单位：中国园艺学会观赏园艺专业委员会、国家花卉工程技术研究中心
- 学会名称：中国园艺学会
- 页数：8
- 文件大小：1182k
- 原文格式：O
- 会议级别：全国
摘要
本研究以长寿花品种‘Don Bombero'的花蕾为外植体,通过对外植体表面灭菌后,研究了暗处理时长、无机盐浓度、激素种类及浓度配比等因素对长寿花花器官离体培养的影响。主要结论为:花蕾外植体最适灭菌方法为75%酒精15s+2%次氯酸钠8min;诱导培养阶段随暗处理时长和激素水平不同同时出现丛生芽和花芽两种诱导结果,适宜丛生芽诱导的暗处理时长为3d,培养基配方为:MS+6-BA1.5mg·L~(-1)+NAA0.3mg·L~(-1),适宜花芽诱导的暗处理时长为14d,培养基配方为:MS+6-BA0.5mg·L~(-1)+NAA0.3mg·L~(-1);丛生芽适宜增殖培养基为:MS+6-BA2.0mg·L~(-1)+NAA0.3mg·L~(-1);适宜壮苗培养基为:1/2MS+NAA0.1mg·L~(-1)+IBA0.3mg·L~(-1)+CCC2~3mg·L~(-1)+AC3g·L~(-1),同时出现试管开花现象。
Using bud of Kalanchoe blossfeldiana ' Don Bombero' as explants,this study was mainly to explore the flower organ tissue culture technology of K.blossfeldiana.Influencing factors such as sterilization methods,length of dark treatment,diverse hormone concentration combinations and mineral salts concentration were investigated.The main results are as follows:The suitable sterilize methond of bud is to sterilize with 75%ethanol for 30 s and then followed by 8 min in 2%NaClO.The induction culture turned out to two results,which were multiple shoots and flower bud when cultured by different dark reatment and hormone combination.The suitable dark reatment for multiple shoots and flower bud was 3 and 14 days,and the suitable induction medium was MS +6-BA1.5mg · L~(-1) + NAA0.3mg · L~(-1) and MS +6-BA0.5mg · L~(-1) + NAAO.3mg · L~(-1),respectively.The suitable multiplication medium was MS + 6-BA2.Omg · L~(-1) + NAA0.3mg · L~(-1).The suitable medium for strengthening was 1/2MS + NAA0.lmg · L~(-1) + IBA0.3mg · L~(-1) + CCC2-3mg · L~(-1) +3g · L~(-1) AC,along with in vitro flowering.
Using bud of Kalanchoe blossfeldiana ' Don Bombero' as explants,this study was mainly to explore the flower organ tissue culture technology of K.blossfeldiana.Influencing factors such as sterilization methods,length of dark treatment,diverse hormone concentration combinations and mineral salts concentration were investigated.The main results are as follows:The suitable sterilize methond of bud is to sterilize with 75%ethanol for 30 s and then followed by 8 min in 2%NaClO.The induction culture turned out to two results,which were multiple shoots and flower bud when cultured by different dark reatment and hormone combination.The suitable dark reatment for multiple shoots and flower bud was 3 and 14 days,and the suitable induction medium was MS +6-BA1.5mg · L~(-1) + NAA0.3mg · L~(-1) and MS +6-BA0.5mg · L~(-1) + NAAO.3mg · L~(-1),respectively.The suitable multiplication medium was MS + 6-BA2.Omg · L~(-1) + NAA0.3mg · L~(-1).The suitable medium for strengthening was 1/2MS + NAA0.lmg · L~(-1) + IBA0.3mg · L~(-1) + CCC2-3mg · L~(-1) +3g · L~(-1) AC,along with in vitro flowering.
引文
1.曹国仪,唐锡华.烟草茎薄层培养直接形成花芽及其极性现象(简报)[J].植物生理学通讯,1988(03):47-49.
2.程军.重瓣长寿花花序离体快繁研究[J].北方园艺,2010(02):166-168.
3.李凤兰,胡国富,杜景红,等.长寿花(Kalanehoe blossfeldiana CV.Tom Thumb)花序芽培养及植株再生[J].东北农业大学学报,2003(03):314-317.
4.罗娅,汤浩茹,邓涛艳,等.长寿花快繁及试管苗开花研究[J].安徽农业科学,2009(06):2389-2390.
5.马国华,刘念.从长寿花嫩叶直接诱导体细胞胚和出芽[J].植物生N-"学通讯,2003(06):625.
6.孙利娜.长寿花嫩叶片的组织培养研究[J].西部林业科学,2011(02):48-51.
7.赵云鹏,郭维明,陆红梅.细胞薄层培养在植物激素调节形态发生研究中的应用[J].植物生理学通讯,2003(04):385-390.
8.郑新会,柳俊,袁继红,等.粉花长寿花试管花卉研究[J].上海农业学报,2013(04):72-74.
2.程军.重瓣长寿花花序离体快繁研究[J].北方园艺,2010(02):166-168.
3.李凤兰,胡国富,杜景红,等.长寿花(Kalanehoe blossfeldiana CV.Tom Thumb)花序芽培养及植株再生[J].东北农业大学学报,2003(03):314-317.
4.罗娅,汤浩茹,邓涛艳,等.长寿花快繁及试管苗开花研究[J].安徽农业科学,2009(06):2389-2390.
5.马国华,刘念.从长寿花嫩叶直接诱导体细胞胚和出芽[J].植物生N-"学通讯,2003(06):625.
6.孙利娜.长寿花嫩叶片的组织培养研究[J].西部林业科学,2011(02):48-51.
7.赵云鹏,郭维明,陆红梅.细胞薄层培养在植物激素调节形态发生研究中的应用[J].植物生理学通讯,2003(04):385-390.
8.郑新会,柳俊,袁继红,等.粉花长寿花试管花卉研究[J].上海农业学报,2013(04):72-74.