无乳链球菌phoB基因缺失菌的构建及其免疫效果评价
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- 英文论文题名：Construction of a Streptcoccus agalactiae phoB mutant and evaluation of its potential as an attenuated modified live vaccine in golden pompano,Trachinotusovatus
- 论文作者：蔡小辉 ; 王蓓 ; 彭银辉 ; 黎源 ; 鲁义善 ; 黄郁葱 ; 简纪常 ; 吴灶和
- 英文论文作者：CAI Xiaohui ; WANG Bei ; LI Yuan ; LUYishan ; HUANGYucong ; JIANJichang ; WUZaohe ; College of Fishery ; Guangdong Ocean University ; Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Insititutes
- 年：2016
- 作者机构：广东海洋大学水产学院,广东省水产经济动物病原生物学及流行病学重点实验室,广东省水产经济动物病害控制重点实验室;广西生物技术重点实验室,广西海洋研究所;
- 论文关键词：无乳链球菌 ; phoB ; 活疫苗 ; 金鲳
- 英文论文关键词：Streptococcus agalactiae ; phoB ; live attenuated vaccine ; golden pompano
- 会议召开时间：2016-11-02
- 会议录名称：2016年中国水产学会学术年会论文摘要集
- 语种：中文
- 分类号：S941.4
- 学会代码：OGSB
- 会议名称：2016年中国水产学会学术年会
- 会议地点：中国四川成都
- 主办单位：中国水产学会、四川省水产学会
- 学会名称：中国水产学会
- 页数：1
- 文件大小：577k
- 原文格式：D
- 会议级别：全国
摘要
无乳链球菌可在吞噬细胞和非吞噬细胞内存活从而引起鱼类败血症和脑膜炎。通过构建高度保守及毒力相关基因的活疫苗可有效控制链球菌病本研究克隆了phoB基因,氨基酸序列比对结果显示该基因与其它种链球菌具有较高相似性,包含两个高度保守的域:REC和trans_reg_C。利用同源重组技术构建了SA?phoB缺失株,与野生菌相比,其细胞黏附和侵入能力及毒力均降低,并且未引起金鲳临床症状和明显的病理改变,表明phoB基因的缺失株影响了无乳链球菌的毒力和侵染能力。通过腹腔注射方式以SA?phoB缺失株为抗原免疫金鲳,结果显示其免疫保护率达93.1%,由此可见该缺失菌可作为一种高效的候选减毒活疫苗株。
To search the highly conserved and virulent-related genes in metabolic pathways for constructing live attenuated vaccine will help to understand and control streptococcosis. In this study, the pho B gene was cloned from pathogenic S. agalactiae TOS01 strain and the mutant strain SA?pho Bwas constructed via allelic exchange mutagenesis. The results showed that the deduced amino acid of S. agalactiae TOS01 shares high similarities with other Streptococcus spp.and has high conserved REC and trans_reg_C domain. Cell adhesion and invasion assay,challenge experiments and histopathological changes post-vaccination were performed and observed, the results showed that the mutant strain SA?pho B has a lower adherence and invasion rate and less virulent than the wild type strain in golden pompano, and it did not induce clinical symptoms and obvious pathological changes in golden pompano, thereby indicating that the deletion of pho B affects the virulence and infectious capacity of S. agalactiae.
To search the highly conserved and virulent-related genes in metabolic pathways for constructing live attenuated vaccine will help to understand and control streptococcosis. In this study, the pho B gene was cloned from pathogenic S. agalactiae TOS01 strain and the mutant strain SA?pho Bwas constructed via allelic exchange mutagenesis. The results showed that the deduced amino acid of S. agalactiae TOS01 shares high similarities with other Streptococcus spp.and has high conserved REC and trans_reg_C domain. Cell adhesion and invasion assay,challenge experiments and histopathological changes post-vaccination were performed and observed, the results showed that the mutant strain SA?pho B has a lower adherence and invasion rate and less virulent than the wild type strain in golden pompano, and it did not induce clinical symptoms and obvious pathological changes in golden pompano, thereby indicating that the deletion of pho B affects the virulence and infectious capacity of S. agalactiae.
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